CN106577507A - Early-period gender judging method for soft-shell turtles - Google Patents
Early-period gender judging method for soft-shell turtles Download PDFInfo
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- CN106577507A CN106577507A CN201611097380.0A CN201611097380A CN106577507A CN 106577507 A CN106577507 A CN 106577507A CN 201611097380 A CN201611097380 A CN 201611097380A CN 106577507 A CN106577507 A CN 106577507A
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- shelled turtle
- soft
- trionyx sinensis
- gender
- turtle trionyx
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 241001482311 Trionychidae Species 0.000 title abstract description 32
- 210000004907 gland Anatomy 0.000 claims abstract description 10
- 238000005259 measurement Methods 0.000 claims abstract description 6
- 238000007619 statistical method Methods 0.000 claims abstract description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 3
- 241000736919 Pelodiscus sinensis Species 0.000 claims description 38
- 241000270708 Testudinidae Species 0.000 claims description 10
- 210000003041 ligament Anatomy 0.000 claims description 4
- 210000000436 anus Anatomy 0.000 claims description 2
- 230000002596 correlated effect Effects 0.000 claims description 2
- 238000007654 immersion Methods 0.000 claims description 2
- 210000000867 larynx Anatomy 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 abstract description 13
- 230000001568 sexual effect Effects 0.000 abstract description 9
- 230000012447 hatching Effects 0.000 abstract description 3
- 230000004069 differentiation Effects 0.000 abstract description 2
- 241000270666 Testudines Species 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 238000005303 weighing Methods 0.000 abstract 1
- 238000003307 slaughter Methods 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 239000010977 jade Substances 0.000 description 3
- 210000001672 ovary Anatomy 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 230000032678 sex differentiation Effects 0.000 description 3
- 241000270609 Caretta caretta Species 0.000 description 2
- 241000270711 Malaclemys terrapin Species 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- VOXZDWNPVJITMN-ZBRFXRBCSA-N 17β-estradiol Chemical compound OC1=CC=C2[C@H]3CC[C@](C)([C@H](CC4)O)[C@@H]4[C@@H]3CCC2=C1 VOXZDWNPVJITMN-ZBRFXRBCSA-N 0.000 description 1
- 241001125669 Lepidochelys olivacea Species 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229960005309 estradiol Drugs 0.000 description 1
- 229930182833 estradiol Natural products 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 230000002710 gonadal effect Effects 0.000 description 1
- 230000023508 male gonad development Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Information Retrieval, Db Structures And Fs Structures Therefor (AREA)
Abstract
An early-period gender judging method for soft-shell turtles relates to the field of gender differentiation and gender regulation of temperature-determining animals. The method comprises the following steps of weighing the soft-shell turtle which was hatched two weeks ago, soaking the soft-shell turtle in alcohol, acquiring and measuring external shape parameters, observing sexual glands of female and male turtles, determining gender of the soft-shell turtle through tissue slice observation, acquiring soft-shell turtle external characteristic parameters which are significantly related with the gender through statistical analysis, obtaining a regression equation and judging the gender of the newly born soft-shell turtle through the regression equation. Quantitative indexes for early-period gender judgment of the soft-shell turtle are presented. Gender judgment can be quickly and accurately performed through external data measurement without animal killing two weeks after hatching of the soft-shell turtle. The early-period gender judging method has advantages of high speed, high accuracy, no requirement for animal killing, and high operability.
Description
Technical field
The present invention relates to the Sex Differentiation of temperature decision type animal and sex phenotype field, more particularly, to a kind of Shelled Turtle Trionyx Sinensis
Early sex authentication method.
Background technology
The Sex Differentiation and study on regulation of testudinate animal is one of current international research focus.The sex of many testudinate animals
Decision is environment decision type, due to greenhouse effects so that global warming is raised thereby results in some species female-male proportion imbalances, very
There is unisexuality to its offspring, cause the extinction of species, therefore it is very urgent to carry out terrapin Sex Differentiation research.Terrapin early stage
Sex identification is the basis of the studies above work.Additionally, early sex is identified for testudinate manufacturing enterprise, it may have very
Important meaning.
The patent for being related to the relevant Shelled Turtle Trionyx Sinensis of this area relates generally to the microsection manufacture of its embryo's sexual gland[1], hatching and sex
Regulation and control[2-6], about the patent of Shelled Turtle Trionyx Sinensis early sex identification report is had no.Primary track is beautiful[7-9]Sex identification to Soft-shelled Turtle
Primary Study is carried out, 6 the Shelled Turtle Trionyx Sinensis more than monthly age have been primarily adapted for use in, has been about the final criterion of young tortoise sex identification both at home and abroad
Carry out histotomy observation to sexual gland, accurately and reliably, but workload is very big for the method, and to slaughter substantial amounts of animal, unfavorable
In animal protection, be not particularly suitable in the research of endangered animal, external some researchers propose by young tortoise spermary with
The method that the surface difference of ovary carries out sex identification[10-11]。
It is existing by Shelled Turtle Trionyx Sinensis outward appearance identify its property method for distinguishing generally require hatch after cultivate the soft-shelled turtle of or so half a year, its
Accuracy rate is generally 80% or so.
Bibliography
[1] Wang Wei etc..A kind of continuous paraffin section preparation method of 2014. Shelled Turtle Trionyx Sinensis embryonic period, embryonic phase sexual glands and its sentence in sex
Application in fixed.Patent application number：201410132534.X, application publication number：CN 103940647, date of publication：
2014.07.23。
[2] Chen Pengfei, Chen Xinchu.The cultural method of a kind of 2014. raising Hanshou soft-shelled turtle egg laying amounts and laying quality.Invention
Application number：20140176843.7, application publication number：CN 103918605, date of publication：2014.07.16.
[3] Huang Hongke, Chen Xinchu, Chen Pengfei etc..A kind of 2014. soft-shelled turtle pedigree breeding methods.Patent application number：
201310340156.X, application publication number：CN 103843722A, date of publication：2014.06.11
[4] Qian Guoying etc..A kind of 2013. Shelled Turtle Trionyx Sinensis embryonic period, embryonic phase male sex derivants and abductive approach.Patent application number：
201210432096.X, application publication number：CN 102988986A, date of publication：2013.03.27.
[5] thank to middle richness etc..2012. a kind of sex control method for Chinese soft-shelled turtles.Patent application number：2011110367548.6, Shen
Please publication No.：CN 102487883A, date of publication：2012.06.13.
[6] the .2013. Shelled Turtle Trionyx Sinensis of Wang three or three hatching sex-control method. patent application number：201210332425.3, application
Publication No.：CN 102934623A, date of publication：2013.02.20.
[7] sex identification [J] of the primary track jade young soft-shelled turtles of .2007.. University Of Hebei's journal, 27 (5):518-520.
[8] development [J] of primary track jade .2008. Shelled Turtle Trionyx Sinensis ovaries. Southwestern University's journal, 30 (4):169-173
[9] histological observation [J] of primary track jade .2009. Shelled Turtle Trionyx Sinensis testis developments. Agriculture of Anhui science, 37 (22):
10522-10524.
[10] Gross T S, Crain DA, Bjorndal KA, et al 1995..Identification of sex
in hatchling loggerhead turtles(Caretta caretta)by analysis of steroid
concentrations in chorioallantoic/amniotic fliuid.General and Comparative
Endocrinology, 99:204-210.
[11]Horacio M L,Salvador R R,Norma M M,et al.1997.Correlation among
thermosensitive period,estradiol response,and gonad differentiation in the
Sea Turtle Lepidochelys olivacea.General and Comparative Endocrinology,107:
373-385.
The content of the invention
Present invention aims to traditional gonadal tissue section or sexual gland visual inspection method, need to slaughter in a large number
Animal, the problems such as workload is huge, there is provided without the need for dissect animal, can by the collection and data analysis of Shelled Turtle Trionyx Sinensis apparent parameter
It is simple, efficient, workable, high with quantitative target, accuracy rate to carry out Shelled Turtle Trionyx Sinensis early stage (going out shell 2 weeks) sex identification
A kind of Shelled Turtle Trionyx Sinensis early sex authentication method.
The present invention's comprises the following steps that：
To go out after the shell Shelled Turtle Trionyx Sinensis of two weeks are weighed with alcohol-pickled, gather and measure formalness parameter, observation male and female are young
Tortoise sexual gland, is observed by histotomy, determines its sex, then by statistical analysis acquisition and the significantly correlated Shelled Turtle Trionyx Sinensis of sex outside
Portion's characteristic parameter, obtains regression equation, and by regression equation the sex of nascent tortoise is judged.
The Shelled Turtle Trionyx Sinensis can adopt 242；The alcohol-pickled immersion that can adopt mass percentage concentration for 75% alcohol
30min；The formalness parameter includes but is not limited to carapace length, carapace width, plastron length, plastron width, body height, tail length etc.；Institute
State collection method can be found in testudinate classification illustrated handbook (week it is graceful. testudinate classify illustrated handbook【M】. Beijing：Chinese agriculture publishing house, 2004：
77-79)；The measurement can adopt slide measure；Measurement includes carapace length, carapace width, plastron length, plastron width, the body of Shelled Turtle Trionyx Sinensis
High, tail length, carapace length is the air line distance of carapace (center line) leading edge to carapace trailing edge；Carapace width is left side ligament lateral border
To the air line distance of the right ligament lateral border；The a length of larynx shield ditch of plastron is to the air line distance between anus shield ditch；A width of two first of plastron
Most long air line distance between bridge；The high vertical height being in the middle part of tortoise body of body；Tail length is the length of plastron base portion to tail end
Degree.
The present invention is by collection, the young tortoise of male and female of the shape parameter to 242 male and female Soft-shelled Turtles (going out shell 2 weeks or so)
The observation of sexual gland and its its sex is finally determined by the observation of its histotomy, obtained and the notable phase of sex by statistical analysis
The Shelled Turtle Trionyx Sinensis surface gain of parameter regression equation of pass, by regression equation the sex of nascent tortoise, accuracy rate are may determine that
Higher than 99%.
Advantages of the present invention and have technical effect that：
The present invention gives first the quantitative target of Shelled Turtle Trionyx Sinensis early sex identification, after soft-shelled turtle goes out shell 2 weeks by be not required to
Slaughter animal, by the measurement of external data, rapidly and accurately (accuracy rate more than 99%) carries out sex identification.Sum up this
Patent of invention is quick, accurate, need not slaughter animal, and the workable Shelled Turtle Trionyx Sinensis early sex with quantitative target is identified
Method.
Description of the drawings
Fig. 1 is male Soft-shelled Turtle dorsal view
Fig. 2 is male Soft-shelled Turtle norma ventralis.
Fig. 3 is female Soft-shelled Turtle dorsal view.
Fig. 4 is female Soft-shelled Turtle norma ventralis.
Fig. 5 is male Shelled Turtle Trionyx Sinensis sexual gland collection of illustrative plates.In Figure 5, white pipe is that (in fusiformis, two ends become spermary gradually
Carefully, come to a point)
Fig. 6 is female Shelled Turtle Trionyx Sinensis sexual gland collection of illustrative plates.In figure 6, white pipe is that (left figure is two ends targe to ovary, right
Figure is one end targe).
Specific embodiment
Embodiment 1：
The collection of external parameter, the female Soft-shelled Turtle back of the body are carried out according to described above to going out shell 242 Shelled Turtle Trionyx Sinensis of 2 weeks
First width (Y) can be expressed as with the relation of carapace length：Y=1.0423+0.6525x (n=122, r=0.7532, F=157.3989
(P<0.01), the relational expression of male both Soft-shelled Turtles is：Y=1.0965+0.6401x (n=120, r=0.7067, F
=117.6961 (P<0.01).
The accuracy rate for differentiating Soft-shelled Turtle sex according to the method described above is higher than 99%.
Embodiment 2：
The collection of external parameter is carried out to going out shell 242 Shelled Turtle Trionyx Sinensis of 2 weeks according to described above, variance analysis shows, female
The wide ratio of male Soft-shelled Turtle carapace width/front carapace is present and significant difference (P<0.01), the ratio of female Soft-shelled Turtle
For 1.34, male for 1.24.Differentiate young soft-shelled turtle sex using the wide ratio of young soft-shelled turtle carapace width/front carapace, accuracy rate is higher than 99%.
Embodiment 3：
The collection of external parameter is carried out to going out shell 242 Shelled Turtle Trionyx Sinensis of 2 weeks according to described above, variance analysis shows, female
There is significant difference (P in the tail length of male Soft-shelled Turtle<0.01), the tail length mean value of female soft-shelled turtle is 0.84cm, and the tail length of male soft-shelled turtle is put down
Average is 0.91cm.Differentiate young soft-shelled turtle sex using the wide ratio of young soft-shelled turtle carapace width/front carapace, accuracy rate is higher than 99%.
Claims (7)
1. a kind of Shelled Turtle Trionyx Sinensis early sex authentication method, it is characterised in that it is comprised the following steps that：
To go out after the shell Shelled Turtle Trionyx Sinensis of two weeks are weighed with alcohol-pickled, gather and measure formalness parameter, the young tortoise property of observation male and female
Gland, is observed by histotomy, determines its sex, then special by the significantly correlated Shelled Turtle Trionyx Sinensis outside of statistical analysis acquisition and sex
Parameter is levied, regression equation is obtained, the sex of nascent tortoise is judged by regression equation.
2. as claimed in claim 1 a kind of Shelled Turtle Trionyx Sinensis early sex authentication method, it is characterised in that the Shelled Turtle Trionyx Sinensis adopt 242
Only.
3. a kind of as claimed in claim 1 Shelled Turtle Trionyx Sinensis early sex authentication method, it is characterised in that the alcohol-pickled employing matter
Amount percentage concentration is the immersion 30min of 75% alcohol.
4. a kind of as claimed in claim 1 Shelled Turtle Trionyx Sinensis early sex authentication method, it is characterised in that the formalness parameter bag
Include but be not limited to carapace length, carapace width, plastron length, plastron width, body height, tail length.
5. as claimed in claim 1 a kind of Shelled Turtle Trionyx Sinensis early sex authentication method, it is characterised in that the method for the collection referring to
Testudinate classification illustrated handbook.
6. as claimed in claim 1 a kind of Shelled Turtle Trionyx Sinensis early sex authentication method, it is characterised in that the measurement adopts vernier calliper
Chi.
7. as claimed in claim 1 a kind of Shelled Turtle Trionyx Sinensis early sex authentication method, it is characterised in that the measurement includes Shelled Turtle Trionyx Sinensis
Carapace length, carapace width, plastron length, plastron width, body be high, tail length, carapace length be the straight line of carapace leading edge to carapace trailing edge away from
From；Carapace width is left side ligament lateral border to the air line distance of the right ligament lateral border；The a length of larynx shield ditch of plastron is to anus shield ditch
Between air line distance；Most long air line distance between a width of two first bridge of plastron；The high vertical height being in the middle part of tortoise body of body；Tail
The long length for being plastron base portion to tail end.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611097380.0A CN106577507A (en) | 2016-12-02 | 2016-12-02 | Early-period gender judging method for soft-shell turtles |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611097380.0A CN106577507A (en) | 2016-12-02 | 2016-12-02 | Early-period gender judging method for soft-shell turtles |
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| CN106577507A true CN106577507A (en) | 2017-04-26 |
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| CN201611097380.0A Pending CN106577507A (en) | 2016-12-02 | 2016-12-02 | Early-period gender judging method for soft-shell turtles |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108048579A (en) * | 2018-01-25 | 2018-05-18 | 湖南生物机电职业技术学院 | PCR amplification primer, method and the kit of Rapid identification Shelled Turtle Trionyx Sinensis genetic sex |
| CN108588237A (en) * | 2018-05-31 | 2018-09-28 | 浙江大学 | Shelled Turtle Trionyx Sinensis sex identification primer sets, site and method |
| CN108633828A (en) * | 2018-03-10 | 2018-10-12 | 凉山彝族自治州畜牧兽医科学研究所 | One stud ram morning age seed choosing method |
| CN109077015A (en) * | 2017-06-14 | 2018-12-25 | 北京市水产科学研究所 | The method for separating of Cuora flavomarginate adult tortoise figure |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5201280A (en) * | 1989-06-06 | 1993-04-13 | Reproductive Sciences, Inc. | Method for preferential production of female turtles, lizards, and crocodiles |
| CN102487883A (en) * | 2011-11-18 | 2012-06-13 | 绍兴中亚工贸园有限公司 | Sex control method for Chinese soft-shelled turtles |
| CN102669048A (en) * | 2012-05-10 | 2012-09-19 | 章成铭 | Core technology for controlling gender in cuora trifasciata incubation |
| CN102763625A (en) * | 2012-07-28 | 2012-11-07 | 湖南农业大学 | Culture method of pseudo-female Chinese soft-shelled turtle and all-male Chinese soft-shelled turtle |
| CN102934623A (en) * | 2012-09-09 | 2013-02-20 | 安徽省旌德县国友龟鳖良种繁育场 | Method for controlling incubation genders of Chinese soft-shelled turtle (CSST) |
| CN102988986A (en) * | 2012-11-02 | 2013-03-27 | 浙江万里学院 | Male trait inducer and induction method for pelodiscus sinensis in embryonic periods |
| CN103940647A (en) * | 2014-04-02 | 2014-07-23 | 浙江万里学院 | Manufacture method of continuous paraffin sections for gonad of pelodiscus sinensis in embryonic period and application of manufacture method in sex determination |
| CN104604779A (en) * | 2015-03-03 | 2015-05-13 | 梧州市景泰养鳖专业合作社 | Gender proportioning method of parent trionyx sinensis pool |
| CN104705251A (en) * | 2013-12-16 | 2015-06-17 | 嵊州市黄缘盒龟有限公司 | Chinese-box-turtle incubation sex temperature control method and system |
| CN105724310A (en) * | 2016-02-16 | 2016-07-06 | 深圳职业技术学院 | Method for judging time phase of embryonic development of Chinese softshell turtles without anatomy |
-
2016
- 2016-12-02 CN CN201611097380.0A patent/CN106577507A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5201280A (en) * | 1989-06-06 | 1993-04-13 | Reproductive Sciences, Inc. | Method for preferential production of female turtles, lizards, and crocodiles |
| CN102487883A (en) * | 2011-11-18 | 2012-06-13 | 绍兴中亚工贸园有限公司 | Sex control method for Chinese soft-shelled turtles |
| CN102669048A (en) * | 2012-05-10 | 2012-09-19 | 章成铭 | Core technology for controlling gender in cuora trifasciata incubation |
| CN102763625A (en) * | 2012-07-28 | 2012-11-07 | 湖南农业大学 | Culture method of pseudo-female Chinese soft-shelled turtle and all-male Chinese soft-shelled turtle |
| CN102934623A (en) * | 2012-09-09 | 2013-02-20 | 安徽省旌德县国友龟鳖良种繁育场 | Method for controlling incubation genders of Chinese soft-shelled turtle (CSST) |
| CN102988986A (en) * | 2012-11-02 | 2013-03-27 | 浙江万里学院 | Male trait inducer and induction method for pelodiscus sinensis in embryonic periods |
| CN104705251A (en) * | 2013-12-16 | 2015-06-17 | 嵊州市黄缘盒龟有限公司 | Chinese-box-turtle incubation sex temperature control method and system |
| CN103940647A (en) * | 2014-04-02 | 2014-07-23 | 浙江万里学院 | Manufacture method of continuous paraffin sections for gonad of pelodiscus sinensis in embryonic period and application of manufacture method in sex determination |
| CN104604779A (en) * | 2015-03-03 | 2015-05-13 | 梧州市景泰养鳖专业合作社 | Gender proportioning method of parent trionyx sinensis pool |
| CN105724310A (en) * | 2016-02-16 | 2016-07-06 | 深圳职业技术学院 | Method for judging time phase of embryonic development of Chinese softshell turtles without anatomy |
Non-Patent Citations (2)
| Title |
|---|
| 李慧君: "中华鳖性腺分化及温度对其影响", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 郑光明 等: "平胸龟年龄与生长及雌雄差异的比较", 《华南农业大学学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109077015A (en) * | 2017-06-14 | 2018-12-25 | 北京市水产科学研究所 | The method for separating of Cuora flavomarginate adult tortoise figure |
| CN108048579A (en) * | 2018-01-25 | 2018-05-18 | 湖南生物机电职业技术学院 | PCR amplification primer, method and the kit of Rapid identification Shelled Turtle Trionyx Sinensis genetic sex |
| CN108633828A (en) * | 2018-03-10 | 2018-10-12 | 凉山彝族自治州畜牧兽医科学研究所 | One stud ram morning age seed choosing method |
| CN108588237A (en) * | 2018-05-31 | 2018-09-28 | 浙江大学 | Shelled Turtle Trionyx Sinensis sex identification primer sets, site and method |
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Application publication date: 20170426 |