CN106576824A - Method of cultivating improved varieties of cannabis through root tips - Google Patents
Method of cultivating improved varieties of cannabis through root tips Download PDFInfo
- Publication number
- CN106576824A CN106576824A CN201611141791.5A CN201611141791A CN106576824A CN 106576824 A CN106576824 A CN 106576824A CN 201611141791 A CN201611141791 A CN 201611141791A CN 106576824 A CN106576824 A CN 106576824A
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- Prior art keywords
- root
- tip
- weight portion
- cannabis
- seedling
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Cell Biology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method of cultivating improved varieties of cannabis through root tips, and relates to the technical field of cannabis plantation technique. The method of cultivating improved varieties of cannabis through root tips is characterized by utilizing cell totipotency of a root tip to cultivate plantlets with roots and leaves from cells of a meristematic zone so as to cultivate improved varieties of cannabis without virus to grow. The method of cultivating improved varieties of cannabis through root tips solves the problem that a method of using traditional seeds to breed cannabis has the disadvantages of being low in the germination capacity of seeds, being long in the accelerating germination time, being inconsistent in the accelerating germination time, being difficult in collecting resource of cannabis seeds, and increasing the difficulty in large scale production of cannabis as all the excellent properties of male parent and female parent are difficult to keep because of variation of the filial generation.
Description
Technical field
The present invention relates to fire fiber crops planting technology field, especially a kind of method of fiery fiber crops of being cultivated fine seed strains by the tip of a root.
Background technology
Fiery fiber crops sapling multiplication technology is mainly that based on generative propagation method, the advantage of seminal propagation is once to broadcast with seminal propagation
Planting can obtain a large amount of nursery stocks, seed collection, storage, convenient transportation, and seedling growth is vigorous, strong stress resistance, easily domestication.But pass
There is following defect in the fire fiber crops seminal propagation of system：(1) percentage of seedgermination is low, and highest only reaches 80% or so；(2) germination time is long
Up to 20 days or so, and germinating time was inconsistent；(3) fire fiber crops resource scarcity, batch capture is difficult；（4）Zoogamy produces variation
Possibility it is high, filial generation possibly cannot retain the maternal whole good characteristics of male parent.The technology of fire fiber crops seminal propagation at present is present
Problem seriously hinders the large-scale production of fiery fiber crops, thus cannot meet demand of the market to fiery fiber crops, it is necessary to which setting up can be a large amount of, fast
The method of speed breeding fire fiber crops seedling.
Plant cell has totipotency, i.e. each plant cell containing all gene that can produce whole plant.It is theoretical
On say, as long as condition is suitable, containing all genetic cells can Differentiation, produce whole plant.Mitogenetic group of the tip of a root
It is usually virion that is nontoxic or only containing extremely low concentration in knitting, and in older tissue, viral level is with a distance from the tip of a root
Increase and improve.Accordingly principle can obtain nontoxic plant using organization of root tips culture.Using this principle, fiery fiber crops can be carried out good
Plant and breed, cultivate virus-free seedling.
The content of the invention
The present invention provides a kind of method of fiery fiber crops of cultivating fine seed strains by the tip of a root, in the way of it can be to solve seminal propagation fire fiber crops
Cannot the numb problem of large-scale production breeding fire.
In order to solve the above problems, the technical solution adopted in the present invention is：It is this to be cultivated fine seed strains fiery fiber crops by the tip of a root
Method, comprises the steps：
A, plant screening：When fiery fiber crops are ripe or during rudiment, the plant of no disease and pests harm is selected；
B, pretreatment and sterilization：1～the 2cm of the tip of a root of step A gained plant is taken, it is clean with clean water, then floated with 70% alcohol
Wash 30s, then with 0.1% HgCl2Solution sterilization 3min, aseptic washing 3 is throughout the above；
C, the tip of a root obtained by step B is placed under microscope, after alcohol-pickled, then the tweezers that cooled down with calcination and scissors are by root
Sharp meristematic zone cell is cut into 3～7 tip of a root segments, then with sterile water wash 3 throughout the above；
D, cultivation：Step C gained tip of a root segment is put in inducing culture carries out culture 15 days～25 days, the induction training
Foster base is MS+6- benzyl purines 1.5mg/L+methyl α-naphthyl acetate 1.0mg/L, obtain it is pre- take root, by the pre- 1～2cm of the tip of a root taken root
It is cut into 3～7 tip of a root segments and accesses the inducing culture culture 15～25 days, repeat the above steps is an appropriate number of to obtaining
After taking root in advance, by it is described it is pre- take root 25～30 for a collection of, proliferated culture medium is accessed in batches, the proliferated culture medium is MS+6-
Benzyl purine 1.5mg/L+ methyl α-naphthyl acetate 1.5mg/L, cultivate to growing 3cm～4 cm Multiple Buds；
E, the seedling of long root that step D gained comes into leaves are put into 3～5 days under sunlight, take out seedling, are transplanted to seedling bed, OK
Away from 5～6cm, 5～6cm of spacing in the rows, deep 3～4cm is opened, one plant of seedling of often place's plantation keeps seedling bed moistening；The seedling bed be by
Walnut husk is mixed with cane mill's filter mud and/or fish pond mud, calcium magnesium phosphate ferments and obtain as matrix；As 60% children
When seedling plant length is to height of seedling 50cm～60cm, land for growing field crops is transplanted to, you can.
In above-mentioned technical proposal, more specifically technical scheme can also be：The condition of culture of the breeding phase is temperature
23～28 DEG C, 1500～1800LUX of intensity of illumination.
Further：Each composition proportion of the seedling bed is weight portion～100 weight portion of walnut husk 80, cane mill
Filter mud and/or weight portion～25 weight portion of fish pond mud 20, weight portion～10 weight portion of dipotassium hydrogen phosphate 5, the weight of magnesium sulfate 5
Part～10 weight portions, weight portion～10 weight portion of zinc sulfate 5.
As a result of above-mentioned technical proposal, the present invention has the advantages that compared with prior art：
1st, the present invention cultivates fire fiber crops by the asexual reproduction method of the tip of a root, and the mode for solving traditional seminal propagation fire fiber crops is brought
Percentage of seedgermination is low, germination time length and germinating time is inconsistent, collection fire fiber crops seed resource is difficult, filial generation is due to variation
The big problem of large-scale production fire fiber crops difficulty caused by maternal whole merit of male parent etc. cannot be retained.
2nd, the present invention can cultivate virus-free breeding fire fiber crops seedling using the cellular omnipotency of the tip of a root, be planted
Plant, plant strain growth is healthy and strong, growing way is consistent, and pest and disease damage incidence is low, and can keep numb all premium properties of wild fire and effective
Component content.
Specific embodiment
Below in conjunction with specific embodiment, the invention will be further described：
The method of this fiery fiber crops of being cultivated fine seed strains by the tip of a root, is comprised the steps：
A, plant screening：When fiery fiber crops are ripe or during rudiment, the plant of no disease and pests harm is selected；
B, pretreatment and sterilization：1～the 2cm of the tip of a root of step A gained plant is taken, it is clean with clean water, then floated with 70% alcohol
Wash 30s, then with 0.1% HgCl2Solution sterilization 3min, aseptic washing 3 is throughout the above；
C, the tip of a root obtained by step B is placed under microscope, after alcohol-pickled, then the tweezers that cooled down with calcination and scissors are by root
Sharp meristematic zone cell is cut into 3 tip of a root segments, then with sterile water wash 3 throughout the above；
D, cultivation：Step C gained tip of a root segment is put in inducing culture carries out culture 15 days, and the inducing culture is
MS+6- benzyl purines 1.5mg/L+methyl α-naphthyl acetate 1.0mg/L, obtain it is pre- take root, the pre- 1～2cm of the tip of a root taken root is cut into into 3
Individual tip of a root segment accesses the inducing culture culture 15, repeat the above steps to obtain it is an appropriate number of it is pre- take root after, by institute
State it is pre- take root 25 for a collection of, proliferated culture medium is accessed in batches, the proliferated culture medium is MS+6- benzyl purine 1.5mg/L+ naphthalenes
Acetic acid 1.5mg/L, cultivates to growing 3cm～4cm Multiple Buds；
Above condition of culture be 23～28 DEG C of temperature, 1500～1800LUX of intensity of illumination；
E, the seedling of long root that step D gained comes into leaves are put into 3～5 days under sunlight, take out seedling, are transplanted to seedling bed, OK
Away from 5～6cm, 5～6cm of spacing in the rows, deep 3～4cm is opened, one plant of seedling of often place's plantation keeps seedling bed moistening；The seedling bed be by
The weight portion of walnut husk 80 is mixed with the weight portion of cane mill's filter mud 10, the weight portion of fish pond mud 10, phosphoric acid hydrogen two as matrix
The weight portion of potassium 5, the weight portion of magnesium sulfate 5, the weight portion of zinc sulfate 5 ferment and obtain；When 60% seedling plants length to height of seedling 50cm～
During 60cm, land for growing field crops is transplanted to, you can.
Claims (3)
1. a kind of method of fiery fiber crops of being cultivated fine seed strains by the tip of a root, it is characterised in that comprise the steps：
A, plant screening：When fiery fiber crops are ripe or during rudiment, the plant of no disease and pests harm is selected；
B, pretreatment and sterilization：1～the 2cm of the tip of a root of step A gained plant is taken, it is clean with clean water, then floated with 70% alcohol
Wash 30s, then with 0.1% HgCl2Solution sterilization 3min, aseptic washing 3 is throughout the above；
C, the tip of a root obtained by step B is placed under microscope, after alcohol-pickled, then the tweezers that cooled down with calcination and scissors are by root
Sharp meristematic zone cell is cut into 3～7 tip of a root segments, then with sterile water wash 3 throughout the above；
D, cultivation：Step C gained tip of a root segment is put in inducing culture carries out culture 15 days～25 days, the induction training
Foster base is MS+6- benzyl purines 1.5mg/L+methyl α-naphthyl acetate 1.0mg/L, obtain it is pre- take root, by the pre- 1～2cm of the tip of a root taken root
It is cut into 3～7 tip of a root segments and accesses the inducing culture culture 15～25 days, repeat the above steps is an appropriate number of to obtaining
After taking root in advance, by it is described it is pre- take root 25～30 for a collection of, proliferated culture medium is accessed in batches, the proliferated culture medium is MS+6-
Benzyl purine 1.5mg/L+ methyl α-naphthyl acetate 1.5mg/L, cultivate to growing 3cm～4 cm Multiple Buds；
E, the seedling of long root that step D gained comes into leaves are put into 3～5 days under sunlight, take out seedling, are transplanted to seedling bed, OK
Away from 5～6cm, 5～6cm of spacing in the rows, deep 3～4cm is opened, one plant of seedling of often place's plantation keeps seedling bed moistening；The seedling bed be by
Walnut husk is mixed with cane mill's filter mud and/or fish pond mud, calcium magnesium phosphate ferments and obtain as matrix；As 60% children
When seedling plant length is to height of seedling 50cm～60cm, land for growing field crops is transplanted to, you can.
2. the method for fiery fiber crops of being cultivated fine seed strains by the tip of a root according to claim 1, it is characterised in that：The breeding phase
Condition of culture be 23～28 DEG C of temperature, 1500～1800LUX of intensity of illumination.
3. the method for fiery fiber crops of being cultivated fine seed strains by the tip of a root according to claim 1, it is characterised in that：The seedling bed it is each
Composition proportion is weight portion～100 weight portion of walnut husk 80, cane mill's filter mud and/or weight portion～25 of fish pond mud 20
Weight portion, weight portion～10 weight portion of dipotassium hydrogen phosphate 5, weight portion～10 weight portion of magnesium sulfate 5, weight portion～10 of zinc sulfate 5
Weight portion.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611141791.5A CN106576824A (en) | 2016-12-12 | 2016-12-12 | Method of cultivating improved varieties of cannabis through root tips |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611141791.5A CN106576824A (en) | 2016-12-12 | 2016-12-12 | Method of cultivating improved varieties of cannabis through root tips |
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| Publication Number | Publication Date |
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| CN106576824A true CN106576824A (en) | 2017-04-26 |
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| CN201611141791.5A Withdrawn CN106576824A (en) | 2016-12-12 | 2016-12-12 | Method of cultivating improved varieties of cannabis through root tips |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108934803A (en) * | 2018-04-12 | 2018-12-07 | 中国农业科学院麻类研究所 | A method of cultivating hemp seedling |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102503610A (en) * | 2011-10-11 | 2012-06-20 | 赵平平 | Method for treating hickory epicarp to produce fertilizer |
| CN106069763A (en) * | 2016-06-25 | 2016-11-09 | 杨文平 | The pierre method of Radix Scrophulariae |
| CN106106150A (en) * | 2016-06-25 | 2016-11-16 | 杨文平 | Cultivated fine seed strains by the tip of a root method of Radix Scrophulariae |
| CN106106151A (en) * | 2016-06-25 | 2016-11-16 | 杨文平 | The prevalent variety cultivation method of Radix Sophorae Flavescentis |
-
2016
- 2016-12-12 CN CN201611141791.5A patent/CN106576824A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102503610A (en) * | 2011-10-11 | 2012-06-20 | 赵平平 | Method for treating hickory epicarp to produce fertilizer |
| CN106069763A (en) * | 2016-06-25 | 2016-11-09 | 杨文平 | The pierre method of Radix Scrophulariae |
| CN106106150A (en) * | 2016-06-25 | 2016-11-16 | 杨文平 | Cultivated fine seed strains by the tip of a root method of Radix Scrophulariae |
| CN106106151A (en) * | 2016-06-25 | 2016-11-16 | 杨文平 | The prevalent variety cultivation method of Radix Sophorae Flavescentis |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108934803A (en) * | 2018-04-12 | 2018-12-07 | 中国农业科学院麻类研究所 | A method of cultivating hemp seedling |
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Application publication date: 20170426 |