CN106576500A - Determination method for viability of calligonum mongolicum seeds - Google Patents
Determination method for viability of calligonum mongolicum seeds Download PDFInfo
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- CN106576500A CN106576500A CN201611219985.2A CN201611219985A CN106576500A CN 106576500 A CN106576500 A CN 106576500A CN 201611219985 A CN201611219985 A CN 201611219985A CN 106576500 A CN106576500 A CN 106576500A
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- seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
Abstract
The invention provides a determination method for the viability of calligonum mongolicum seeds. The determination method comprises the step of calligonum mongolicum seed sampling and further comprises the following follow-up treatment steps of a clarity treatment, b seed presoaking treatment, c skin incising and embryo exposing, d seed soaking treatment, e first-time counting of the seeds with the viability, f seed soaking continuing treatment and g last-time counting of the viability and result calculating which are conducted on the calligonum mongolicum seeds. According to the method, whether the calligonum mongolicum seeds have the viability or not is judged according to the fact of whether radicle is obviously elongated or not and whether the elongating length of the radicle is larger than or equal to 2 mm. Compared with an existing germination test method, the determination method has the advantages that the determination time is shortened by 35 days, and the determination cost is reduced; compared with an existing viability tetrazole dyeing determination method, the determination method has the advantages that the defect that embryos are prone to be damaged in the embryo exposing step is overcome, the identification standard is intuitive, the result is reliable, operation is easy and convenient, and adopted chemical reagents are non-toxic, environmentally friendly, economical and practical.
Description
Technical field
The invention belongs to seed quality identification and cultivation field, more particularly to the measure side of a planting sand honey raisin tree seed vigor
Method.
Background technology
Calligonum (Calligonum) is polygonaceae (Polygonaceae) plant, is distributed in Asia, south of europe and non-
Continent is northern.The whole world has 35 kinds, and China possesses 23 kinds, wherein 8 kinds is endemic species.Husky honey raisin tree is mainly widely distributed in China,
Xinjiang, the Inner Mongol, Gansu, Ningxia and Qinghai are distributed.
The platymiscium is often born in desert or the desert edges such as flowing, semi-mobile dune, Desert Regions sand area, drought resistance
By force, anti-blown sand, is resistant to light, moderate saline-alkali environment.Arid-desert area ecological recovery with rebuild in be widely used.
The platymiscium is both excellent sand-fixation plant, forage plant, and with economic uses such as medicinal, nectar source, yule logs.Ring is adapted to long-term
During border, the platymiscium defines special external structure and hereditary capacity, be possess the raw gene of drought germ plasm resource it
One, it is listed in national key protected wild plants.
Because the platymiscium has the good ecological value, feeding value and potential economic worth, extensively cultivated.
The seed fruit-setting rate of Calligonum plant is higher, but due to its living environment it is severe, seed in growth course often because of water shortage or
Other reason maturity not enough, or develop imperfection, cause the viability extremely unstable of seed, and germination percentage is low.So far, have
Close the detection of husky honey raisin tree seed quality and the rarely seen Inner Mongolia Autonomous Region provincial standard of method evaluated《Husky honey raisin tree seed grading and inspection
Test》(DB15/366-2001).Wherein define the germination test method of Calligonum alaschanicum (C.alaschanicum) seed.
Whole germination test time needs 42 days in the method, including advance freezing processing 21 days, germination test 21 days.Test period is too
It is long, and in advance freezing processing does not ensure that viable seed is all sprouted, so as to underestimate the viability of seed.The method
Preprocess method is that the shortcoming of the method is not at rip cutting embryo 3/4 before the dyeing that middle viability tetrazolium assay method is recommended
Embryo can be fully exposed, Color is uneven, cause result of determination accuracy poor.Therefore, a kind of equipment or method are developed,
Can quickly, accurate evaluation sand honey raisin tree seed vigor it is very necessary.
The content of the invention
In view of this, the technical problem to be solved in the present invention is the defect for overcoming prior art, there is provided a planting sand honey raisin tree
The assay method of seed vigor.Skewer being carried out first and taking husky honey raisin tree seed step, the assay method also includes turning the sand
Jujube seed carries out following subsequent processing steps：
A) cleanliness process step：Clean and obtain the net seed of husky honey raisin tree；
B) soak seed in advance process step：The net seed that random skewer takes is placed in the vessel for filling hydrogenperoxide steam generator, constant temperature,
Soak seed under dark condition；
C) Qie Pilu embryos step：The seed of immersion is taken out, is rinsed well, laterally cut at the tip of seed and account for kind of a subpopulation
The achene of product 1/6～2/6；The achene tip is the one end containing radicle；
D) seed soaking step：The seed of dew embryo is again placed in the vessel for filling hydrogenperoxide steam generator, constant temperature, dark
Under the conditions of soak；
E) the first statistic procedure of viable seed：The seed of immersion is taken out, is rinsed well, counted radicle and stretch out length
The quantity of the seed of degree >=2mm, as the first statistics for having viability seed；
F) seed soaking step is continued：During the seed of taking-up to be all placed in again the vessel for filling hydrogenperoxide steam generator,
Continue to soak seed under constant temperature, dark condition；
G) viability last statistics and result calculation procedure：The seed of immersion is taken out, is rinsed well, radicle is counted respectively and is stretched
Go out length >=2mm, the quantity of the seed that radicle extension elongation ＜ 2mm and radicle do not stretch out, as the last statistics of test；Embryo
The seed of root extension elongation >=2mm is used as viable seed；Calculating has viability seed to account for the percentage of seed of participating in the experiment.
In some embodiments, the step c), it in mass fraction is 0.5～1.5% that the pre- seed soaking process is
In hydrogenperoxide steam generator, in 20 DEG C of constant temperature, soak seed 22～26 hours under dark condition.
In some embodiments, in the step c), it is to use scalpel at achene tip that the Qie Pilu embryos are processed
Transversely cut and account for the 1/6～2/6 of achene total length.
In some embodiments, the step d), the seed soaking process is in the mistake that mass fraction is 0.5～1.5%
In hydrogen peroxide solution, in 20 DEG C of constant temperature, soak seed 34～38 hours under dark condition.
In some embodiments, the step e), the seed soaking process is in the mistake that mass fraction is 0.5～1.5%
In hydrogen peroxide solution, in 20 DEG C of constant temperature, soak seed 46～50 hours under dark condition.
In some embodiments, the step b), the pre- seed soaking process is in the hydrogen peroxide that mass fraction is 1%
In solution, in 20 DEG C of constant temperature, soak seed 24 hours under dark condition.
In some embodiments, the step d), the immersion process is molten in the hydrogen peroxide that mass fraction is 1%
In liquid, in 20 DEG C of constant temperature, soak 36 hours under dark condition.
The assay method of husky honey raisin tree seed vigor that the present invention is provided is relative to the beneficial effect of prior art：This
It is bright be in order to solve the problems, such as prior art in assay method overlong time and poor reliability problem, there is provided it is a kind of more simple
Just more structurally sound seed vigor assay method.
Radicle is one of chief component of seed plant embryo.The close germination hole in the tip of radicle, when seed is sprouted,
Usually radicle is dashed forward break in the seed coat first, develops into the main root of seedling.
Embryo is the initial body of plant.It is made up of plumule, cotyledon, plumular axis and the part of radicle four.Plumular axis is connection plumule, cotyledon
With the part of radicle；Plumule is rudimentary sprout, in the cotyledon of the tip of embryo；Cotyledon is located at the upper end of embryo.Naked sub- plant
Thing has multiple cotyledons；Then it is divided into dicotyledon and the big class of monocotyledon two in angiosperm.In a word, embryo is contained in seed
It is interior, it is the plant young in a dormant state.
Whether the present invention appears according to radicle, i.e., whether the extended length of radicle is more than 2mm judging the seed of husky honey raisin tree
Whether viability is had.The viability test time is shortened compared with existing germination test method, efficiency is improve, survey is reduced
Determine expense；Standard of perfection is more intuitive and reliable compared with tetrazolium dyeing assay method, and easy to operate, chemical reagent used is nontoxic
Property, it is more environmentally-friendly and economical and practical.Additive method, such as grenz ray method are compared, simple and easy to do, principle reliability is repeatable
It is good.
In sum, the assay method of the husky honey raisin tree seed vigor that the present invention is provided has the advantages that above-mentioned many and valency
It is worth, and similar method is there are no in like product and publish or use and really belong to innovation, generates handy and practical
Effect, more existing technology has the multinomial effect promoted, so as to more be suitable to practicality, and with extensive industrial value.
Specific embodiment
For the ease of understanding the present invention, technical scheme is elaborated with reference to the mode of embodiment,
Elaborate many details in order to fully understand the present invention in the following description.
But the present invention can be to be much different from alternate manner described here implementing, and those skilled in the art can be with
Similar improvement is done in the case of without prejudice to intension of the present invention, therefore the present invention is not embodied as being limited by following public.
As used herein, term：
" one ", " one kind " and " described " are used interchangeably and refer to one or more.
"and/or" is used to represent that one of illustrated situation or both may to occur, and for example, A and/or B includes (A
And B) and (A or B)；
In addition, the scope stated by end points herein includes all numerical value (for example, 1 to 10 bag included in the range of this
Include 1.4,1.9,2.33,5.75,9.98 etc.).
In addition, the statement of " at least one " herein include one and the above all numbers (for example, at least 2, at least
4, at least 6, at least 8, at least 10, at least 25, at least 50, at least 100 etc.).
There is provided the assay method of a planting sand honey raisin tree seed vigor, skewer is carried out first and takes husky honey raisin tree seed step, it is described
Assay method also includes carrying out following subsequent processing steps to the husky honey raisin tree seed：
A) cleanliness process step：Clean and obtain the net seed of husky honey raisin tree；
B) soak seed in advance process step：The net seed that random skewer takes is placed in the vessel for filling hydrogenperoxide steam generator, constant temperature,
Soak seed under dark condition；
C) Qie Pilu embryos step：After pre- seed soaking, rinse well, laterally cut at the tip of seed and account for seed cumulative volume
1/6～2/6 achene；The achene tip is the one end containing radicle；
D) seed soaking step：The seed of dew embryo is again placed in the vessel for filling hydrogenperoxide steam generator, constant temperature, dark
Under the conditions of soak；
E) the first statistic procedure of viable seed：The seed of immersion is taken out, is rinsed well, counted radicle and stretch out length
The quantity of the seed of degree >=2mm, as the first statistics for having viability seed；
F) seed soaking step is continued：During the seed of taking-up to be all placed in again the vessel for filling hydrogenperoxide steam generator,
Continue to soak seed under constant temperature, dark condition；
G) viability last statistics and result calculation procedure：The seed of immersion is taken out, is rinsed well, radicle is counted respectively and is stretched
Go out length >=2mm, the quantity of the seed that radicle extension elongation ＜ 2mm and radicle do not stretch out, as the last statistics of test；Embryo
The seed of root extension elongation >=2mm is used as viable seed；Calculating has viability seed to account for the percentage of seed of participating in the experiment.
Above-mentioned, the sprouting of the hydrogenperoxide steam generator of appropriate mass fraction to husky honey raisin tree seed has facilitation.To main radicle
Elongation have facilitation.
It is understood that the tip of seed (achene) laterally cuts and accounts for seed cumulative volume 1/6～2/6 after pre- seed soaking
Achene；The achene tip is the one end containing radicle；
It is understood that hydrogenperoxide steam generator can be played as the oxygen source of active oxygen in the vital movement of organism
Important function.Husky honey raisin tree seed is processed with exogenous hydrogen peroxide solution, result of the test shows, the hydrogenperoxide steam generator of suitable concentration
The sprouting and growth of husky honey raisin tree seed can be promoted, the germination rate of husky honey raisin tree seed can be effectively improved.
According to experimental data, it is possible to use criterion of the parameter in table 1 as husky honey raisin tree seed vigor.
The hydrogen peroxide determination method seed vigor criterion of table 1
At the end of experiment, count has the number of viability seed respectively in each repetition, has calculated viability seed and has accounted for
Participate in the experiment the percentage of seed.Then calculate the mean percentage of 4 repetitions, in round figures, then to corresponding viability in table 2
Maximum allowable gap corresponding to mean value is compared.
For example, 400 husky honey raisin tree kinds are selected, 4 groups, 100 per group are randomly divided into.By said method calculated 4
The viability test value of individual repetition is respectively 56%, 60%, 58% and 62%.Be calculated 4 repetitions mean percentage (with
Integer representation) it is 60%.By learning that maximum allowable gap must not when viability mean percentage is 60% in consult table 2
More than 19.The viability test value maximum of 4 repetitions is 62% in experimental result, and minimum of a value is 56%, and actual gap is 8,
Without departing from the numerical value 19 of maximum allowable gap.Illustrate measurement result reliability.Viability for planting experimentally subsample can use 4 repetitions
Percentage mean value representing.
The hydrogen peroxide determination of table 2 tests the maximum allowable gap (two position-findings of 2.5% level of signifiance) of 4 repetitions.
In some embodiments of the invention, the pre- seed soaking process is in the hydrogen peroxide that mass fraction is 0.5～1.5%
In solution, in 20 DEG C of constant temperature, soak seed 22～26 hours under dark condition.
In some embodiments of the invention, in the step c), it is to use scalpel in achene that the Qie Pilu embryos are processed
Tip transversely cuts and accounts for the 1/6～2/6 of achene total length.
In some embodiments of the invention, the step d), the seed soaking process be mass fraction be 0.5～1.5%
Hydrogenperoxide steam generator in, in 20 DEG C of constant temperature, under dark condition soak seed 34～38 hours.
In some embodiments of the invention, the step e), the seed soaking process be mass fraction be 0.5～1.5%
Hydrogenperoxide steam generator in, in 20 DEG C of constant temperature, under dark condition soak seed 46～50 hours.
In some embodiments of the invention, the step b), the pre- seed soaking process is in the peroxide that mass fraction is 1%
In changing hydrogen solution, in 20 DEG C of constant temperature, soak seed 24 hours under dark condition.
In some embodiments of the invention, the step d), the immersion process is in the peroxidating that mass fraction is 1%
In hydrogen solution, in 20 DEG C of constant temperature, soak 36 hours under dark condition.
For the ease of understanding the present invention, with reference to embodiment technical scheme is further illustrated.Applicant
Statement, the present invention illustrates the detailed process equipment of the present invention and technological process, but the present invention not office by above-described embodiment
It is limited to above-mentioned detailed process equipment and technological process, that is, does not mean that the present invention should rely on above-mentioned detailed process equipment and technique stream
Cheng Caineng is implemented.Person of ordinary skill in the field it will be clearly understood that any improvement in the present invention, to each original of product of the present invention
The equivalence replacement of material and the addition of auxiliary element, the selection of concrete mode etc., all fall within protection scope of the present invention and open model
Within enclosing.
Embodiment 1
The assay method of one planting sand honey raisin tree seed vigor, carries out first skewer and takes husky honey raisin tree seed step, the measure side
Method also includes carrying out following subsequent processing steps to the husky honey raisin tree seed：
A) cleanliness process step：Clean and obtain the net seed of husky honey raisin tree；
B) soak seed in advance process step：The net seed that random skewer takes is placed in the vessel for filling hydrogenperoxide steam generator, constant temperature,
Soak seed under dark condition；The pre-soak period be in the hydrogenperoxide steam generator that mass fraction is 0.5%, in 20 DEG C of constant temperature,
Soak 22 hours under dark condition.
C) Qie Pilu embryos step：The seed of immersion is taken out, is rinsed well, laterally cut at the tip of seed and account for kind of a subpopulation
The achene of product 1/6；The achene tip is the one end containing radicle；
D) seed soaking step：The seed of dew embryo is again placed in the vessel for filling hydrogenperoxide steam generator, constant temperature, dark
Under the conditions of soak；The immersion process is in the hydrogenperoxide steam generator that mass fraction is 0.5%, in 20 DEG C of constant temperature, dark bar
Soak 34 hours under part.
E) the first statistic procedure of viable seed：The seed of immersion is taken out, is rinsed well, counted radicle and stretch out length
The quantity of the seed of degree >=2mm, as the first statistics for having viability seed；
F) seed soaking step is continued：During the seed of taking-up to be all placed in again the vessel for filling hydrogenperoxide steam generator,
Continue to soak seed under dark condition；The immersion process is in the hydrogenperoxide steam generator that mass fraction is 0.5%, in 20 DEG C of perseverances
Soak 46 hours under temperature, dark condition.
G) viability last statistics and result calculation procedure：The seed of immersion is taken out, is rinsed well, counted radicle and stretch out length
The quantity of the seed of degree >=2mm, as viable seed；Calculating has viability seed to account for the percentage of seed of participating in the experiment.
Embodiment 2
The assay method of one planting sand honey raisin tree seed vigor.Skewer is carried out first takes husky honey raisin tree seed step, the measure side
Method also includes carrying out following subsequent processing steps to the husky honey raisin tree seed：
A) cleanliness process step：Clean and obtain the net seed of husky honey raisin tree；
B) soak seed in advance process step：The net seed that random skewer takes is placed in the vessel for filling hydrogenperoxide steam generator, constant temperature,
Soak seed under dark condition；The pre-soak period be in the hydrogenperoxide steam generator that mass fraction is 1.5%, in 20 DEG C of constant temperature,
Soak 26 hours under dark condition.
C) Qie Pilu embryos step：The seed of immersion is taken out, is rinsed well, laterally cut at the tip of seed and account for kind of a subpopulation
The achene of product 2/6；The achene tip is the one end containing radicle；
D) seed soaking step：The seed of dew embryo is again placed in the vessel for filling hydrogenperoxide steam generator, constant temperature, dark
Under the conditions of soak；The immersion process is in the hydrogenperoxide steam generator that mass fraction is 1.5%, in 20 DEG C of constant temperature, dark bar
Soak 38 hours under part.
E) the first statistic procedure of viable seed：The seed of immersion is taken out, is rinsed well, counted radicle and stretch out length
The quantity of the seed of degree >=2mm, as the first statistics for having viability seed；
F) seed soaking step is continued：During the seed of taking-up to be all placed in again the vessel for filling hydrogenperoxide steam generator,
Continue to soak seed under constant temperature, dark condition；The immersion process is in the hydrogenperoxide steam generator that mass fraction is 1.5%, in black
The lower 20 DEG C of constant temperature of dark condition soaks 50 hours.
G) viability last statistics and result calculation procedure：The seed of immersion is taken out, is rinsed well, counted radicle and stretch out length
The quantity of the seed of degree >=2mm, as viable seed；Calculating has viability seed to account for the percentage of seed of participating in the experiment.
Embodiment 3
The assay method of one planting sand honey raisin tree seed vigor.Skewer is carried out first takes husky honey raisin tree seed step, the measure side
Method also includes carrying out following subsequent processing steps to the husky honey raisin tree seed：
A) cleanliness process step：Clean and obtain the net seed of husky honey raisin tree；
B) soak seed in advance process step：The net seed that random skewer takes is placed in the vessel for filling hydrogenperoxide steam generator, constant temperature,
Soak seed under dark condition；The pre-soak period be in the hydrogenperoxide steam generator that mass fraction is 1.0%, in 20 DEG C of constant temperature,
Soak 24 hours under dark condition.
C) Qie Pilu embryos step：The seed of immersion is taken out, is rinsed well, laterally cut at the tip of seed and account for kind of a subpopulation
The achene of product 1/6～2/6；The achene tip is the one end containing radicle；
D) seed soaking step：The seed of dew embryo is again placed in the vessel for filling hydrogenperoxide steam generator, constant temperature, dark
Under the conditions of soak；The immersion process is in the hydrogenperoxide steam generator that mass fraction is 1.0%, in 20 DEG C of constant temperature, dark bar
Soak 36 hours under part.
E) the first statistic procedure of viable seed：The seed of immersion is taken out, is rinsed well, counted radicle and stretch out length
The quantity of the seed of degree >=2mm, as the first statistics for having viability seed；
F) seed soaking step is continued：During the seed of taking-up to be all placed in again the vessel for filling hydrogenperoxide steam generator,
Continue to soak seed under constant temperature, dark condition；The immersion process is in the hydrogenperoxide steam generator that mass fraction is 1.0%, in 20
Soak 48 hours under DEG C constant temperature, dark condition.
G) viability last statistics and result calculation procedure：The seed of immersion is taken out, is rinsed well, counted radicle and stretch out length
The quantity of the seed of degree >=2mm, as viable seed；Calculating has viability seed to account for the percentage of seed of participating in the experiment.
Claims (7)
1. the assay method of a planting sand honey raisin tree seed vigor, carries out first skewer and takes husky honey raisin tree seed step, it is characterised in that institute
Stating assay method also includes carrying out following subsequent processing steps to the husky honey raisin tree seed：
A) cleanliness process step：Clean and obtain the net seed of husky honey raisin tree；
B) soak seed in advance process step：The net seed that random skewer takes is placed in the vessel for filling hydrogenperoxide steam generator, constant temperature, dark
Under the conditions of soak seed；
C) Qie Pilu embryos step：The seed of immersion is taken out, is rinsed well, laterally cut at the tip of seed and account for seed cumulative volume 1/
6～2/6 achene；The achene tip is the one end containing radicle；
D) seed soaking step：The seed of dew embryo is again placed in the vessel for filling hydrogenperoxide steam generator, constant temperature, dark condition
Lower immersion；
E) there is the first statistic procedure of viability seed：The seed of immersion is taken out, is rinsed well, counting radicle extension elongation >=
The quantity of the seed of 2mm, as the first statistics for having viability seed；
F) seed soaking step is continued：During the seed of taking-up to be all placed in again the vessel for filling hydrogenperoxide steam generator, constant temperature,
Continue to soak seed under dark condition；
G) viability last statistics and result calculation procedure：The seed of immersion is taken out, is rinsed well, radicle is counted respectively and stretches out length
The quantity of the seed that degree >=2mm, radicle extension elongation ＜ 2mm and radicle do not stretch out, as the last statistics of test；Radicle is stretched
Go out the seed of length >=2mm as viable seed；Calculating has viability seed to account for the percentage of seed of participating in the experiment.
2. the assay method of husky honey raisin tree seed vigor according to claim 1, it is characterised in that the step b), institute
It is 20 DEG C of constant temperature, under dark condition in mass fraction is the hydrogenperoxide steam generator between 0.5～1.5% to state pre- seed soaking process
Seed soaking 22～26 hours.
3. the assay method of husky honey raisin tree seed vigor according to claim 1, it is characterised in that in the step c),
It is transversely to cut to account for seed cumulative volume 1/6～2/6 in achene tip by scalpel that the Qie Pilu embryos are processed.
4. the assay method of husky honey raisin tree seed vigor according to claim 1, it is characterised in that the step d), institute
It is 20 DEG C of constant temperature in the hydrogenperoxide steam generator that mass fraction is 0.5～1.5% to state seed soaking process, and under dark condition 34 are soaked
～38 hours.
5. the assay method of husky honey raisin tree seed vigor according to claim 1, it is characterised in that the step f), institute
It is 20 DEG C of constant temperature in the hydrogenperoxide steam generator that mass fraction is 0.5～1.5% to state immersion process, and under dark condition 46 are soaked
～50 hours.
6. the assay method of husky honey raisin tree seed vigor according to claim 4, it is characterised in that the step b), institute
It is that in the hydrogenperoxide steam generator that mass fraction is 1.0%, in 20 DEG C of constant temperature, immersion 24 is little under dark condition to state pre-soak period
When.
7. the assay method of husky honey raisin tree seed vigor according to claim 4, it is characterised in that the step d), institute
It is in the hydrogenperoxide steam generator that mass fraction is 1%, in 20 DEG C of constant temperature, to soak 36 hours under dark condition to state immersion process.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611219985.2A CN106576500A (en) | 2016-12-26 | 2016-12-26 | Determination method for viability of calligonum mongolicum seeds |
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| CN201611219985.2A CN106576500A (en) | 2016-12-26 | 2016-12-26 | Determination method for viability of calligonum mongolicum seeds |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110073760A (en) * | 2019-05-31 | 2019-08-02 | 兰州大学 | A kind of prewetting liquid quickly measured for Iris tenuifolia seed vigor, dyeing liquor and method |
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| CN101502227A (en) * | 2009-03-17 | 2009-08-12 | 中国科学院新疆生态与地理研究所 | Seedling cultivation method for Calligonum mongolicum in sand waste land |
| CN104904376A (en) * | 2015-06-05 | 2015-09-16 | 山东省农业可持续发展研究所 | Method for rapidly detecting viability of aeluropus littoralis seeds |
| CN105123300A (en) * | 2015-09-16 | 2015-12-09 | 广西壮族自治区农业科学院蔬菜研究所 | Identification and evaluation method of cold tolerance in germination period of momordica charantia |
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| CN101502227A (en) * | 2009-03-17 | 2009-08-12 | 中国科学院新疆生态与地理研究所 | Seedling cultivation method for Calligonum mongolicum in sand waste land |
| CN104904376A (en) * | 2015-06-05 | 2015-09-16 | 山东省农业可持续发展研究所 | Method for rapidly detecting viability of aeluropus littoralis seeds |
| CN105123300A (en) * | 2015-09-16 | 2015-12-09 | 广西壮族自治区农业科学院蔬菜研究所 | Identification and evaluation method of cold tolerance in germination period of momordica charantia |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110073760A (en) * | 2019-05-31 | 2019-08-02 | 兰州大学 | A kind of prewetting liquid quickly measured for Iris tenuifolia seed vigor, dyeing liquor and method |
| CN110073760B (en) * | 2019-05-31 | 2020-07-24 | 兰州大学 | Pre-wetting solution, staining solution and method for rapidly determining vitality of Iris tenuifolia seeds |
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