CN105123300A - Identification and evaluation method of cold tolerance in germination period of momordica charantia - Google Patents
Identification and evaluation method of cold tolerance in germination period of momordica charantia Download PDFInfo
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- CN105123300A CN105123300A CN201510586625.5A CN201510586625A CN105123300A CN 105123300 A CN105123300 A CN 105123300A CN 201510586625 A CN201510586625 A CN 201510586625A CN 105123300 A CN105123300 A CN 105123300A
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- germination
- balsam pear
- cold tolerance
- identification
- evaluation method
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The invention discloses an identification and evaluation method of cold tolerance in the germination period of momordica charantia. The germination force, germination rate, vigor index and hypocotyledonary axis length of a material at low temperature are determined, and then the cold tolerance in the germination period of momordica charantia is identified and evaluated through principal component analysis in combination with cluster analysis. Because multiple indexes closely related to the germination period are adopted at the same time, the essential tolerance of momordica charantia can be truly reflected when tolerance evaluation is performed on a large quantity of materials through the method; because the principal component analysis is adopted in combination with cluster analysis for scientific comprehensive evaluation, identification accuracy and reliability are improved, and the method is suitable for identification and evaluation of the cold tolerance of different momordica charantia resources in the germination period and beneficial for accelerating breeding of cold-tolerant momordica charantia varieties.
Description
Technical field
The invention belongs to balsam pear germplasm identification and assessment technique field, particularly relate to a kind of balsam pear germination period Identification of Cold Tolerance and evaluation method.
Background technology
Balsam pear (MomordicacharantiaL.) originates from the torrid areas in Southeast Asia, is distributed widely in the torrid zone, subtropics and Temperate Region in China, happiness high temperature, belong to cold sensitivity vegetables, germination thermophilic is about 30 DEG C, germinates slowly, less than 13 DEG C difficulties of germinateing below 20 DEG C.In spring ahead of time cultivation, manually can heat and carry out Pregermination and seedling breeding, but in poverty-stricken area, culture facility is simple and easy, grasp bad germination temperature, easily cause seed germination rate low, reduce seed availability, improve production cost.Therefore, the improved seeds that the incubation bud phase is also low temperature resistant are necessary.And carry out the Identification of Cold Tolerance that breeding material is primarily carried out in cold resistance breeding, at present for the single index of the many employings of balsam pear bud phase Identification of Cold Tolerance, and the index that the germinating method adopted is formed can not embody seed has and form field run plant ability, thus objectively can not reflect the bud phase cold resistance of resource, have some limitations.
Summary of the invention
The technical problem to be solved in the present invention is to provide good, the of good reliability balsam pear germination period Identification of Cold Tolerance of a kind of accuracy and evaluation method, this method can reflect the patience essence of balsam pear resource truly, be applicable to the Evaluation and identification of different balsam pear resource bud phase cold resistance, be beneficial to and accelerate the cold-resistant breeding of balsam pear.
For solving the problems of the technologies described above, the present invention is by the following technical solutions: balsam pear germination period Identification of Cold Tolerance and evaluation method, measure the germination vigor of material under cryogenic conditions, germination rate, vitality index and hypocotyl length, then by adopting principal component analysis in conjunction with cluster analysis, Identification of Cold Tolerance and evaluation are carried out to balsam pear germination period.
Under cryogenic conditions, the germination vigor of material, germination rate, vitality index and hypocotyl length are germination vigor, germination rate, vitality index and hypocotyl length under 20 DEG C of conditions respectively.
Above-mentioned balsam pear germination period Identification of Cold Tolerance and evaluation method, comprise the following steps:
(1) seed disinfection;
(2) germination box prepares;
(3) mensuration of germination vigor;
(4) mensuration of germination rate and vitality index;
(5) data processing;
(6) overall merit.
Step (1) is undertaken by following operation: seed of bitter gourd is soaked 10min with 3% bleaching water and carries out seed disinfection, then rinse well with clear water.
Step (2) is undertaken by following operation: by the moistening rear loading germination box of sand of sterilizing, under being placed in 20 DEG C of conditions in advance, making the temperature of sand reach 20 DEG C and just carry out dibbling.
Step (3) is undertaken by following operation: the Seed Levels after sterilization be placed in sand surface and cover the thin sand of one deck, 6d adds up germination rate, and using 6d germination rate data as each material germination vigor under cryogenic, calculate with the percentage that the seed number revealing more than radicle 2mm accounts for for planting experimentally subnumber.
Step (4) is undertaken by following operation: the seed that every day all will germinate in step (3), click and enter the germination box other end and be covered with the thick sand of 1cm, 10d adds up field run plant number, and germination rate is the percentage that the field run plant of 10d accounts for for planting experimentally son; Measure the hypocotyl length of normal seedling, calculate vitality index, vitality index is the germination rate of 10d and the product of hypocotyl length simultaneously.
Step (5) is undertaken by following operation: carry out principal component analysis to experimental data, the comprehensive cold-resistant score value computing formula of material bud phase under acquisition cryogenic conditions.
Step (6) is undertaken by following operation: initial data is carried out cluster analysis, and the sequence according to comprehensive cold-resistant score value is classified to cluster result, finally makes Evaluation and identification to balsam pear bud phase cold resistance.
For the limitation that unicity index qualification balsam pear material bud phase cold resistance exists, we have established a kind of balsam pear germination period Identification of Cold Tolerance and evaluation method, measure the germination vigor of material under cryogenic conditions, germination rate, vitality index and hypocotyl length, then by adopting principal component analysis in conjunction with cluster analysis, Identification of Cold Tolerance and evaluation are carried out to balsam pear germination period.Owing to have employed closely-related multiple index with the bud phase simultaneously, thus when applying the present invention and carrying out patience qualification to lot of materials, the patience essence of balsam pear crop can be reflected truly, principal component analysis is adopted to carry out science integration evaluation in conjunction with cluster analysis, improve accuracy and the reliability of qualification, be applicable to the Evaluation and identification of different balsam pear resource bud phase cold resistance, be beneficial to and accelerate the cold-resistant breeding of balsam pear.
Accompanying drawing explanation
Fig. 1 cluster analysis figure.
Embodiment
Embodiment
1. for examination material
Thered is provided by Guangxi Academy of Agricultural Sciences's Vegetable Research Institute for examination balsam pear material.
2. test method and result
(1) seed disinfection
Seed of bitter gourd is soaked 10min with 3% bleaching water (active ingredient is the aqueous sodium hypochlorite solution of 5%) and carries out seed disinfection, then rinse well with clear water.
(2) germination box prepares
By in the moistening rear loading germination box of sand of sterilizing, under being placed in 20 DEG C of conditions in advance, making the temperature of sand reach 20 DEG C and just carry out dibbling test.
(3) mensuration (under 20 DEG C of conditions) of germination vigor
Seed Levels after sterilization be placed in sand surface and cover the thin sand of one deck, 6d adds up germination rate, and using 6d germination rate data as each material germination vigor under cryogenic, (subnumber × 100% is planted experimentally in germination rate (%)=chitting piece number/confession, and the germination rate calculated can be regarded as the germination vigor of each material) is calculated with the percentage that the seed number revealing more than radicle 2mm accounts for for planting experimentally subnumber.
This assay method is undertaken by the sand method of burying in GB/T3543.4-1995 " crop seeds inspection procedure germination test ", but germination vigor statistical method is slightly changed, and to reveal the seed percent basis of more than radicle 2mm, instead of forms field run plant meter; And owing to being germinate under cryogenic, first a couple of days number extend to 6d by the 4d specified in code, and using the germination rate just counted as the germination vigor of seed.Now the germination vigor of seed does not represent the ability possessing and form field run plant.
(4) mensuration (under 20 DEG C of conditions) of germination rate and vitality index
The seed that every day all will germinate in step (3), click and enter the germination box other end and be covered with the thick sand of 1cm, 10d adds up field run plant number, germination rate is the percentage that the field run plant of 10d accounts for for planting experimentally son, and subnumber × 100% is planted experimentally in germination rate (%)=formation field run plant number/confession; Measure the hypocotyl length (unit is cm) of normal seedling, calculate vitality index, vitality index is the germination rate of 10d and the product (vitality index=germination rate × hypocotyl length) of hypocotyl length (cm) simultaneously.
This assay method is undertaken by the sand method of burying in GB/T3543.4-1995 " crop seeds inspection procedure germination test ", and germination rate now shows as the ability possessing and form field run plant.
(5) data processing (material measured value and cold resistance sort)
SPSS19.0 is utilized to carry out principal component analysis, according to principal component analysis method, the comprehensive cold-resistant score value computing formula of material bud phase under acquisition cryogenic conditions:
F＝0.662F
1+0.192F
2，
Wherein F
1=0.37X
1+ 0.532X
2+ 0.589X
3+ 0.482X
4;
F
2＝0.871X
1+0.038X
2-0.237X
3-0.433X
4，
F
1represent the first principal component value; F
2represent the second main component values; X
1, X
2, X
3, X
4represent respectively germination vigor, germination rate, vitality index and hypocotyl length measured value be described after standardized value.
The cold-resistant score value of each material and rank are in table 1.In table 1, the numerical value of first four is respectively the original measured value of the germination vigor of each material, germination rate, Seed Integrated vigor Index, hypocotyl length.From numerical analysis intuitively, under cryogenic, the germination vigor of 6d is the seed percent basis revealing more than radicle 2mm, and the seed of dew more than radicle 2mm might not possess the ability forming field run plant; The germination rate of 10d forms field run plant ratio statistics, illustrates that seed possesses to germinate and form field run plant ability; But under adverse environmental factor, the seed possessing high germination ability not necessarily possesses high seed vitality, and high vigor seed has obvious growth vigor and productive potential, Seed Integrated vigor Index is the product of germination rate and hypocotyl length, can show the height of seed vitality.Comprehensive main component values is directly proportional to resource cold resistance.
The original measured value of table 1 material and cold resistance sort
Therefore, the indexs relevant to the bud phase such as germination vigor, germination rate, Seed Integrated vigor Index, hypocotyl length are not only closely connected but also independent to some extent, balsam pear material bud phase cold resistance all has certain limitation to utilize any one unicity index to identify, reduce reliability and the accuracy of Evaluation and identification, the cold resistance essence of material can not be reflected.
(6) overall merit
Initial data is carried out cluster analysis, and the sequence according to comprehensive cold-resistant score value is classified to cluster result, finally makes Evaluation and identification to balsam pear bud phase cold resistance.
As shown in Figure 1, be 5 places at squared euclidean distance, can be divided into 4 types by for examination material, the first kind is height low-temperature sensitive type, and Equations of The Second Kind is low-temperature sensitive type, and the 3rd class is highly resistance to cold mould, and the 4th class is resistance to cold mould.
Claims (9)
1. a balsam pear germination period Identification of Cold Tolerance and evaluation method, it is characterized in that: measure the germination vigor of material under cryogenic conditions, germination rate, vitality index and hypocotyl length, then by adopting principal component analysis in conjunction with cluster analysis, Identification of Cold Tolerance and evaluation are carried out to balsam pear germination period.
2. balsam pear germination period Identification of Cold Tolerance according to claim 1 and evaluation method, is characterized in that: under described cryogenic conditions, the germination vigor of material, germination rate, vitality index and hypocotyl length are germination vigor, germination rate, vitality index and hypocotyl length under 20 DEG C of conditions respectively.
3. balsam pear germination period Identification of Cold Tolerance according to claim 1 and evaluation method, is characterized in that comprising the following steps:
(1) seed disinfection;
(2) germination box prepares;
(3) mensuration of germination vigor;
(4) mensuration of germination rate and vitality index;
(5) data processing;
(6) overall merit.
4. balsam pear germination period Identification of Cold Tolerance according to claim 3 and evaluation method, is characterized in that step (1) is undertaken by following operation: seed of bitter gourd is soaked 10min with 3% bleaching water and carries out seed disinfection, then rinse well with clear water.
5. balsam pear germination period Identification of Cold Tolerance according to claim 3 and evaluation method, it is characterized in that step (2) is undertaken by following operation: by the moistening rear loading germination box of sand of sterilizing, under being placed in 20 DEG C of conditions in advance, making the temperature of sand reach 20 DEG C and just carry out dibbling.
6. balsam pear germination period Identification of Cold Tolerance according to claim 3 and evaluation method, it is characterized in that step (3) is undertaken by following operation: the Seed Levels after sterilization be placed in sand surface and cover the thin sand of one deck, 6d adds up germination rate, and using 6d germination rate data as each material germination vigor under cryogenic, calculate with the percentage that the seed number revealing more than radicle 2mm accounts for for planting experimentally subnumber.
7. balsam pear germination period Identification of Cold Tolerance according to claim 3 and evaluation method, it is characterized in that step (4) is undertaken by following operation: the seed that every day all will germinate in step (3), click and enter the germination box other end and be covered with the thick sand of 1cm, 10d adds up field run plant number, and germination rate is the percentage that the field run plant of 10d accounts for for planting experimentally son; Measure the hypocotyl length of normal seedling, calculate vitality index, vitality index is the germination rate of 10d and the product of hypocotyl length simultaneously.
8. balsam pear germination period Identification of Cold Tolerance according to claim 3 and evaluation method, it is characterized in that step (5) is undertaken by following operation: principal component analysis is carried out to experimental data, the comprehensive cold-resistant score value computing formula of material bud phase under acquisition cryogenic conditions.
9. balsam pear germination period Identification of Cold Tolerance according to claim 3 and evaluation method, it is characterized in that step (6) is undertaken by following operation: initial data is carried out cluster analysis, sequence according to comprehensive cold-resistant score value is classified to cluster result, finally makes Evaluation and identification to balsam pear bud phase cold resistance.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106576500A (en) * | 2016-12-26 | 2017-04-26 | 兰州大学 | Determination method for viability of calligonum mongolicum seeds |
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2015
- 2015-09-16 CN CN201510586625.5A patent/CN105123300A/en active Pending
Non-Patent Citations (5)
| Title |
|---|
| 刘斌 等: "盐碱胁迫对不同苦瓜品种种子萌发的影响", 《安徽农业科学》 * |
| 国家技术监督局: "《GB/T 3543.4-1995 农作物种子检验规程发芽试验》", 1 November 1995, 中国标准出版社 * |
| 姜羽: "温度对苦瓜种子发芽的影响试验", 《甘肃科技》 * |
| 苗永美 等: "黄瓜萌芽期和苗期耐冷性评价", 《应用生态学报》 * |
| 黄玉辉 等: "不同苦瓜品种种子发芽和苗期耐冷性差异的研究", 《长江蔬菜（学术版）》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106576500A (en) * | 2016-12-26 | 2017-04-26 | 兰州大学 | Determination method for viability of calligonum mongolicum seeds |
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Application publication date: 20151209 |