CN106565479A - Extracting method for chlorogenic acid in globe artichoke - Google Patents
Extracting method for chlorogenic acid in globe artichoke Download PDFInfo
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- CN106565479A CN106565479A CN201610933131.4A CN201610933131A CN106565479A CN 106565479 A CN106565479 A CN 106565479A CN 201610933131 A CN201610933131 A CN 201610933131A CN 106565479 A CN106565479 A CN 106565479A
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- C07—ORGANIC CHEMISTRY
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- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
- C07C67/52—Separation; Purification; Stabilisation; Use of additives by change in the physical state, e.g. crystallisation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
- C07C67/56—Separation; Purification; Stabilisation; Use of additives by solid-liquid treatment; by chemisorption
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C67/00—Preparation of carboxylic acid esters
- C07C67/48—Separation; Purification; Stabilisation; Use of additives
- C07C67/58—Separation; Purification; Stabilisation; Use of additives by liquid-liquid treatment
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Abstract
The invention relates to an extracting method for chlorogenic acid in globe artichoke. The extracting method comprises the following steps of: cleaning leaves of globe artichoke, drying the leaves of globe artichoke in air, smashing the leaves of the globe artichoke, extracting the smashed leaves of the globe artichoke with an alcohol solution at a temperature ranging from 60 DEG C to 90 DEG C, and removing alcohol by carrying out vacuum concentration at a temperature ranging from 40 DEG C to 80 DEG C; mixing an obtained product with water, volume of which is three times that of the obtained product, to obtain a mixture, extracting the mixture with petroleum ether, and regulating a pH value of a water phase to 5.5-6.5; extracting the mixture with ethyl ether, and regulating the pH value of the water phase to 1.5-2.5; extracting the mixture with ethyl ether, and getting an organic phase; removing ethyl ether by carrying out vacuum concentration on the organic phase; adding an alcohol aqueous solution into a product with ethyl ether removed, and crystallizing at a low temperature; filtering a crystallized product and getting filter residues; and drying the filter residues. According to the extracting method for chlorogenic acid in globe artichoke provided by the invention, an extracting process for chlorogenic acid is simplified while high-purity chlorogenic acid is obtained, so that production cost of the extracting process of chlorogenic acid is reduced.
Description
Technical field
The present invention relates to technical field of natural product extraction, and in particular to a kind of extracting method of globe artichoke Content of Chlorogenic Acid.
Background technology
Chlorogenic acid, chemical name CA；With antibacterial, anti-inflammatory, cholagogic, diuresis, lipid-loweringing, step-down, resist
Oxidation, it is antitumor to wait several functions.There are some researches show again, chlorogenic acid also has necessarily at aspects such as anti-AIDS, treatment diabetes
Curative effect.
Globe artichoke another name arithoke, cynara scolymus, French lily, originate in the area between north African and Mediterranean the east, there is thousand of
The plantation history in year.Globe artichoke has very high nutritive value, is a kind of high-grade health-care vegetable, and its leaf contains cynarin, green original
Acid etc. are with liver protection, protect liver, the active skull cap components for reducing cholesterol effect.There are some researches show, containing more in globe artichoke
Polyphenol compound, wherein with the content highest of chlorogenic acid.
For the chlorogenic acid in globe artichoke in prior art, extraction process is complicated and product yield is low；Therefore, explore efficient
The technique for extracting globe artichoke Content of Chlorogenic Acid is imperative.
The content of the invention
For defect of the prior art, the present invention is intended to provide a kind of extracting method of globe artichoke Content of Chlorogenic Acid, with
Obtain simplifying the extraction process of chlorogenic acid while high-purity chlorogenic acid, so as to reduce the production cost of the extraction process of chlorogenic acid.
For this purpose, the present invention provides a kind of extracting method of globe artichoke Content of Chlorogenic Acid, comprise the following steps：S101：By Korea
Ji leaf cleans, dry after crush, the leaf of Cynara scolymus L alcoholic solution after crushing is extracted into 30min-300min at 60 DEG C -90 DEG C,
Then the removing alcohol concentrated in vacuo at 40 DEG C -80 DEG C；S102：After the product that S101 is obtained mixes with the water of 3 times of volumes, stone is used
Oily ether extraction, regulation aqueous pH values are 5.5-6.5；S103：Solution ether of the pH value for 5.5-6.5 is extracted, water phase is adjusted
PH value is 1.5-2.5, and solution ether of the pH value for 1.5-2.5 is extracted, and takes organic phase；S104：Organic phase in S103 is true
Sky concentration removes ether；S105：Ethanol water is added in the product that S104 is obtained, is crystallized at subzero 10 DEG C -5 DEG C；
S106：Product after crystallization is filtered, filter residue is taken；By filter residue and drying.
Chlorogenic acid, chemical name CA；With antibacterial, anti-inflammatory, cholagogic, diuresis, lipid-loweringing, step-down, resist
Oxidation, it is antitumor to wait several functions.There are some researches show again, chlorogenic acid also has necessarily at aspects such as anti-AIDS, treatment diabetes
Curative effect.Leaf of Cynara scolymus L is cleaned, dry after crush, the leaf of Cynara scolymus L alcoholic solution after crushing is extracted at 60 DEG C -90 DEG C
30min-300min, the active ingredient in leaf of Cynara scolymus L is dissolved in alcoholic solution；It is concentrated in vacuo at 40 DEG C -80 DEG C to be carried with removing
Solvent alcohol during taking；After the product that S101 is obtained mixes with the water of 3 times of volumes, with petroleum ether extraction, to remove extraction
Nonpolar esters in thing etc.；The product that S101 is obtained mixes with the water of 3 times of volumes, i.e. the body of the product that S101 is obtained and water
Product is than being 1:3；Adjust aqueous pH values and be respectively 5.5-6.5, extracted with ether, regulation aqueous pH values are 1.5-2.5, i.e., by second
The water phase of the different pH value of ether extraction, removes the impurity outside chlorogenic acid；Solution of the aqueous pH values for 1.5-2.5 is extracted with ether
Take, take organic phase；Then it is organic phase is concentrated in vacuo removing solvent ether；Product and ethanol water after solvent will be removed
Mixing, crystallizes at subzero 10 DEG C -5 DEG C；Wherein, it can be 1 to remove the product after solvent with the weight ratio of ethanol water:1-
1:5；Under conditions of subzero 10 DEG C -5 DEG C, chlorogenic acid is insoluble in cold alcohol solution, and then can be very good to crystallize out；Will knot
Product after crystalline substance is filtered, you can obtain high-purity chlorogenic acid.
That is, the extracting method of the globe artichoke Content of Chlorogenic Acid of present invention offer, while high-purity chlorogenic acid is obtained
The extraction process of chlorogenic acid is simplified, so as to reduce the production cost of the extraction process of chlorogenic acid.
In the further embodiment of the present invention, in S103, after pH value is for the solution ether extraction of 5.5-6.5,
It is before 1.5-2.5, also including step to adjust aqueous pH values：Regulation aqueous pH values are 4.5-5.5；By pH value for 4.5-5.5's
Solution is extracted with ether, and regulation aqueous pH values are 3.5-4.5；Product with diethyl ether of the pH value for 3.5-4.5 is extracted, water phase is adjusted
PH value is 2.5-3.5；Product with diethyl ether of the pH value for 2.5-3.5 is extracted.
In the further embodiment of the present invention, in S103, after pH value is for the solution ether extraction of 5.5-6.5,
It is before 1.5-2.5, also including step to adjust aqueous pH values：It is 5.0 to adjust aqueous pH values；By the solution second that pH value is 5.0
Ether is extracted, and it is 4.0 to adjust aqueous pH values；By the product with diethyl ether extraction that pH value is 4.0, it is 3.0 to adjust aqueous pH values；By pH value
Product with diethyl ether for 3.0 is extracted.
In the further embodiment of the present invention, in S102：It is 6.0 to adjust aqueous pH values；In S103, it is by pH value
6.0 solution is extracted with ether, and it is 2.0 to adjust aqueous pH values, by the solution ether extraction that pH value is 2.0, takes organic phase.
In the further embodiment of the present invention, in S101, extracted by Soxhlet extraction device.
In the further embodiment of the present invention, alcoholic solution is methanol solution or ethanol solution.
The present invention further embodiment in, using acid solution adjust aqueous pH values, and acid solution be hydrochloric acid solution,
One or more in sulfuric acid solution, salpeter solution and phosphoric acid solution.
In the further embodiment of the present invention, in S102, with petroleum ether extraction specifically, using isopyknic petroleum ether
Extraction 3 times；In S134, it is specially with ether extraction：Extracted 3 times with isopyknic ether.
The present invention further embodiment in, in S104, by organic phase in S103 vacuum be 0.1MPa-
0.07MPa, temperature are 30 DEG C -60 DEG C, the time is concentrated in vacuo to carry out under conditions of 30min-100min.
Description of the drawings
Fig. 1 is the flow chart of the extracting method of the globe artichoke Content of Chlorogenic Acid in the embodiment of the present invention.
Specific embodiment
The embodiment of technical solution of the present invention is described in detail below in conjunction with accompanying drawing.Following examples are only used for
Technical scheme is more clearly illustrated, therefore is only used as example, and the protection model of the present invention can not be limited with this
Enclose.
Fig. 1 is the flow chart of the extracting method of the globe artichoke Content of Chlorogenic Acid in the embodiment of the present invention, as shown in figure 1, this
The extracting method of the globe artichoke Content of Chlorogenic Acid of bright offer, comprises the following steps：
S101：Leaf of Cynara scolymus L is cleaned, dry after crush, by the leaf of Cynara scolymus L alcoholic solution after crushing at 60 DEG C -90 DEG C
Lower extraction 30min-300min, then the removing alcohol concentrated in vacuo at 40 DEG C -80 DEG C.Wherein, carried out by Soxhlet extraction device
Extract；Alcoholic solution is methanol solution or ethanol solution.Chlorogenic acid, chemical name CA；With antibacterial, disappear
Inflammation, cholagogic, diuresis, lipid-loweringing, step-down is anti-oxidant, antitumor to wait several functions.There are some researches show again, chlorogenic acid in anti-AIDS,
The aspects such as treatment diabetes also have certain curative effect.Leaf of Cynara scolymus L is cleaned, dry after crush, by after crushing leaf of Cynara scolymus L use
Alcoholic solution extracts 30min-300min at 60 DEG C -90 DEG C, and the active ingredient in leaf of Cynara scolymus L is dissolved in alcoholic solution；40
At DEG C -80 DEG C it is concentrated in vacuo to remove extraction process in solvent alcohol.
S102：After the product that S101 is obtained mixes with the water of 3 times of volumes, with petroleum ether extraction, adjusting aqueous pH values is
5.5-6.5.Wherein, it is 6.0 to adjust aqueous pH values；With petroleum ether extraction specifically, with isopyknic petroleum ether extraction 3 times.
S103：Solution ether of the pH value for 5.5-6.5 is extracted, regulation aqueous pH values are 1.5-2.5, are by pH value
The solution of 1.5-2.5 is extracted with ether, takes organic phase.Wherein, aqueous pH values are adjusted using acid solution, and acid solution is that hydrochloric acid is molten
One or more in liquid, sulfuric acid solution, salpeter solution and phosphoric acid solution；It is specially with ether extraction：Use isopyknic ether
Extraction 3 times.
With petroleum ether extraction, to remove extract in nonpolar esters etc.；Adjust aqueous pH values and be respectively 5.5-6.5,
Extracted with ether, regulation aqueous pH values are 1.5-2.5, i.e., the water phase of different pH value is extracted by ether, removed outside chlorogenic acid
Impurity；Solution ether of the aqueous pH values for 1.5-2.5 is extracted, organic phase is taken, you can obtains organic rich in chlorogenic acid
Phase.
S104：By the removing ether concentrated in vacuo of organic phase in S103.Wherein, it is in vacuum by organic phase in S103
0.1MPa-0.07MPa, temperature are 30 DEG C -60 DEG C, the time is concentrated in vacuo to carry out under conditions of 30min-100min.
S105：Ethanol water is added in the product that S104 is obtained, is crystallized at subzero 10 DEG C -5 DEG C.
S106：Product after crystallization is filtered, filter residue is taken；By filter residue and drying.
The product removed after solvent ether is mixed with ethanol water, is crystallized at subzero 10 DEG C -5 DEG C；Subzero 10
Under conditions of DEG C -5 DEG C, chlorogenic acid is insoluble in cold alcohol solution, and then can be very good to crystallize out；By the product mistake after crystallization
Filter, you can obtain high-purity chlorogenic acid.
Preferably, in S103, after pH value is for the solution ether extraction of 5.5-6.5, regulation aqueous pH values are 1.5-2.5
Before, also including step：Regulation aqueous pH values are 4.5-5.5；Solution ether of the pH value for 4.5-5.5 is extracted, water is adjusted
Phase pH value is 3.5-4.5；Product with diethyl ether of the pH value for 3.5-4.5 is extracted, regulation aqueous pH values are 2.5-3.5；By pH value
Product with diethyl ether for 2.5-3.5 is extracted.Using substep gradually condition aqueous pH values, and the method extracted with ether, can be by
Secondary removing impurity component, obtains more high-purity chlorogenic acid.
Illustrate with reference to specific embodiment：
Embodiment one
The present invention provides the extracting method of globe artichoke Content of Chlorogenic Acid, comprises the following steps：After leaf of Cynara scolymus L is cleaned, drying
Crush, the leaf of Cynara scolymus L after crushing extracted after 100min by Soxhlet extraction device with 75% ethanol solution at 65 DEG C,
The removing ethanol concentrated in vacuo at 45 DEG C；After product after removing ethanol concentrated in vacuo is mixed with the water of 3 times of volumes, the body such as use
Long-pending petroleum ether extraction, adopts sulfuric acid to adjust aqueous pH values for 6.5；The solution that pH value is 6.5 is extracted with isopyknic ether,
It is 1.5 to adjust aqueous pH values；Then the water that pH value is 1.5 is mutually extracted with isopyknic ether, takes organic phase；Ether is organic
It is 0.1MPa in vacuum, temperature is lower heating 60min under conditions of 35 DEG C, to remove ether；Product after ether is removed
The mass concentration that 5 times of weight are added in thing is 90% ethanol water, is crystallized at -8 DEG C；Product after crystallization is filtered,
Take filter residue；By filter residue and drying.Jing Structural Identifications, products therefrom is really chlorogenic acid.
Embodiment two
The present invention provides the extracting method of globe artichoke Content of Chlorogenic Acid, comprises the following steps：After leaf of Cynara scolymus L is cleaned, drying
Crush, the leaf of Cynara scolymus L after crushing is extracted after 60min, at 55 DEG C by Soxhlet extraction device with methanol solution at 90 DEG C
Removing methyl alcohol concentrated in vacuo；After product after removing methyl alcohol concentrated in vacuo is mixed with the water of 3 times of volumes, isopyknic oil is used
Ether is extracted, and adopts hydrochloric acid to adjust aqueous pH values for 6.0；The solution that pH value is 6.0 is extracted with isopyknic ether, water phase is adjusted
PH value is 2.0；Then the water that pH value is 2.0 is mutually extracted with isopyknic ether, takes organic phase；By ether organic phase in vacuum
Spend for 0.09MPa, temperature is to heat 80min under conditions of 55 DEG C, to remove ether；3 are added in product after ether is removed
The mass concentration of times weight is 90% ethanol water, is crystallized at -10 DEG C；Product after crystallization is filtered, filter residue is taken；Will
Filter residue and drying.Jing Structural Identifications, products therefrom is really chlorogenic acid.
Embodiment three
The present invention provides the extracting method of globe artichoke Content of Chlorogenic Acid, comprises the following steps：After leaf of Cynara scolymus L is cleaned, drying
Crush, the leaf of Cynara scolymus L after crushing is extracted after 60min, at 55 DEG C by Soxhlet extraction device with methanol solution at 90 DEG C
Removing methyl alcohol concentrated in vacuo；After product after removing methyl alcohol concentrated in vacuo is mixed with the water of 3 times of volumes, isopyknic oil is used
Ether is extracted, and adopts hydrochloric acid to adjust aqueous pH values for 6.0；The solution that pH value is 6.0 is extracted with isopyknic ether, water phase is adjusted
PH value is 5.0；By the solution ether extraction that pH value is 5.0, it is 4.0 to adjust aqueous pH values；By the product second that pH value is 4.0
Ether is extracted, and it is 3.0 to adjust aqueous pH values, and the product with diethyl ether that pH value is 3.0 is extracted, and is adjusted aqueous pH values and is
2.0；Then the water that pH value is 2.0 is mutually extracted with isopyknic ether, takes organic phase；It is in vacuum by ether organic phase
0.09MPa, temperature be 55 DEG C under conditions of heat 80min, to remove ether；23 times of weights are added by removing in the product after ether
The mass concentration of amount is 90% ethanol water, is crystallized at -10 DEG C；Product after crystallization is filtered, filter residue is taken；By filter residue
It is dried.Jing Structural Identifications, products therefrom is really chlorogenic acid.
Example IV
The present invention provides the extracting method of globe artichoke Content of Chlorogenic Acid, comprises the following steps：After leaf of Cynara scolymus L is cleaned, drying
Crush, the leaf of Cynara scolymus L after crushing extracted after 200min by Soxhlet extraction device with 60% ethanol solution at 63 DEG C,
The removing ethanol concentrated in vacuo at 75 DEG C；After product after removing ethanol concentrated in vacuo is mixed with the water of 3 times of volumes, the body such as use
Long-pending petroleum ether extraction, adopts nitric acid to adjust aqueous pH values for 5.8；The solution that pH value is 5.8 is extracted with isopyknic ether,
It is 4.8 to adjust aqueous pH values；By the solution ether extraction that pH value is 4.8, it is 3.7 to adjust aqueous pH values；It is 3.7 by pH value
Product with diethyl ether is extracted, and it is 2.7 to adjust aqueous pH values；The product with diethyl ether that pH value is 2.7 is extracted, water phase is adjusted
PH value is 1.7；Then the water that pH value is 1.7 is mutually extracted with isopyknic ether, takes organic phase；By ether organic phase in vacuum
Spend for 0.07MPa, temperature is to heat 90min under conditions of 45 DEG C, to remove ether；Addition etc. in the product after ether will be removed
60% ethanol water of weight, crystallizes at -3 DEG C；Product after crystallization is filtered, filter residue is taken；By filter residue and drying.Jing structures
Identification, products therefrom is really chlorogenic acid.
In addition, by the purity of each product in high-efficient liquid phase chromatogram technique analysis embodiment one to example IV, it is concrete such as table 1
It is shown：Specifically, the experiment condition of high performance liquid chromatography is：
The high performance liquid chromatographs of Waters 2695, chromatographic column：C18 posts (250mm × 4.0mm, 5 μm), mobile phase methanol：
Water：36% acetic acid (45：50：5) (volume ratio), flow velocity 0.7mL/min, Detection wavelength 327nm, 30 DEG C of column temperature, the μ of sampling volume 5
L。
The purity of each embodiment product of table 1
| Embodiment one | Embodiment two | Embodiment three | Example IV | |
| Purity/% | 80 | 83 | 97 | 95 |
As can be seen here, in the extracting method of globe artichoke Content of Chlorogenic Acid of the present invention, the purity of product chlorogenic acid can reach
97%；And in extraction process of the present invention, raw material is simple and easy to get, operating process is simple, can significantly reduce chlorogenic acid extraction process
In cost.
The extracting method of the globe artichoke Content of Chlorogenic Acid that the present invention is provided, simplifies green original while high-purity chlorogenic acid is obtained
The extraction process of acid, so as to reduce the production cost of the extraction process of chlorogenic acid.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means to combine specific features, structure, material or spy that the embodiment or example are described
Point is contained at least one embodiment of the present invention or example.In this manual, to the schematic representation of above-mentioned term not
Identical embodiment or example must be directed to.And, the specific features of description, structure, material or feature can be with office
Combine in an appropriate manner in one or more embodiments or example.Additionally, in the case of not conflicting, the skill of this area
Art personnel can be tied the feature of the different embodiments or example described in this specification and different embodiments or example
Close and combine.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, changes, replacing and modification.
Claims (9)
1. a kind of extracting method of globe artichoke Content of Chlorogenic Acid, it is characterised in that comprise the following steps：
S101：Leaf of Cynara scolymus L is cleaned, dry after crush, by the leaf of Cynara scolymus L alcoholic solution after the crushing at 60 DEG C -90 DEG C
Lower extraction 30min-300min, then the removing alcohol concentrated in vacuo at 40 DEG C -80 DEG C；
S102：After the product that S101 is obtained mixes with the water of 3 times of volumes, with petroleum ether extraction, regulation aqueous pH values are 5.5-
6.5；
S103：Solution ether of the pH value for 5.5-6.5 is extracted, regulation aqueous pH values are 1.5-2.5, by the pH value
Solution ether for 1.5-2.5 is extracted, and takes organic phase；
S104：By the removing ether concentrated in vacuo of organic phase described in S103；
S105：Ethanol water is added in the product that S104 is obtained, is crystallized at subzero 10 DEG C -5 DEG C；
S106：Product after crystallization is filtered, filter residue is taken；By the filter residue and drying.
2. the extracting method of globe artichoke Content of Chlorogenic Acid according to claim 1, it is characterised in that：
In the S103, after the pH value is for the solution ether extraction of 5.5-6.5, the regulation aqueous pH values are 1.5-
Before 2.5, also including step：
Regulation aqueous pH values are 4.5-5.5；Solution ether of the pH value for 4.5-5.5 is extracted, adjusting aqueous pH values is
3.5-4.5；Product with diethyl ether of the pH value for 3.5-4.5 is extracted, regulation aqueous pH values are 2.5-3.5；By the pH value
Product with diethyl ether for 2.5-3.5 is extracted.
3. the extracting method of globe artichoke Content of Chlorogenic Acid according to claim 2, it is characterised in that：
In the S103, after the pH value is for the solution ether extraction of 5.5-6.5, the regulation aqueous pH values are 1.5-
Before 2.5, also including step：
It is 5.0 to adjust aqueous pH values；By the solution ether extraction that the pH value is 5.0, it is 4.0 to adjust aqueous pH values；By institute
The product with diethyl ether extraction that pH value is 4.0 is stated, it is 3.0 to adjust aqueous pH values；By the product with diethyl ether extraction that the pH value is 3.0
Take.
4. the extracting method of globe artichoke Content of Chlorogenic Acid according to claim 1, it is characterised in that：
In the S102：It is 6.0 to adjust aqueous pH values；
In the S103：By the solution ether extraction that the pH value is 6.0, it is 2.0 to adjust aqueous pH values, is by the pH value
2.0 solution is extracted with ether, takes organic phase.
5. the extracting method of globe artichoke Content of Chlorogenic Acid according to claim 1, it is characterised in that：
In the S101, extracted by Soxhlet extraction device.
6. the extracting method of globe artichoke Content of Chlorogenic Acid according to claim 1, it is characterised in that：
In the S101, the alcoholic solution is methanol solution or ethanol solution.
7. the extracting method of globe artichoke Content of Chlorogenic Acid according to claim 1, it is characterised in that：
Aqueous pH values are adjusted using acid solution, and the acid solution is hydrochloric acid solution, sulfuric acid solution, salpeter solution and phosphoric acid solution
In one or more.
8. the extracting method of globe artichoke Content of Chlorogenic Acid according to claim 1, it is characterised in that：
In the S102, with petroleum ether extraction specifically, with isopyknic petroleum ether extraction 3 times；
In the S103, it is specially with ether extraction：Extracted 3 times with isopyknic ether.
9. the extracting method of globe artichoke Content of Chlorogenic Acid according to claim 1, it is characterised in that：
In the S104, by organic phase described in S103 vacuum be 0.1MPa-0.07MPa, temperature be 30 DEG C -60 DEG C, when
Between it is concentrated in vacuo to carry out under conditions of 30min-100min.
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| CN112479881A (en) * | 2020-11-18 | 2021-03-12 | 浏阳朗林生物科技有限公司 | Preparation method of globe artichoke extract |
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Application publication date: 20170419 |