CN106554919A - Microorganism gene detection analyzer - Google Patents
Microorganism gene detection analyzer Download PDFInfo
- Publication number
- CN106554919A CN106554919A CN201611087661.8A CN201611087661A CN106554919A CN 106554919 A CN106554919 A CN 106554919A CN 201611087661 A CN201611087661 A CN 201611087661A CN 106554919 A CN106554919 A CN 106554919A
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- CN
- China
- Prior art keywords
- sample operation
- room
- storehouses
- gene detection
- operation room
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
Abstract
The invention relates to a microbial gene detection analyzer, which comprises a cabin A and a cabin B; the A-bin terminal control chamber controls the vortex mixing and rotating device, the constant-temperature water bath, the high-speed freezing centrifuge and the first sample operation chamber; the input end of the second sample operation chamber of the chamber B is connected with the output end of the first sample operation chamber; the master control room controls the second sample operation room, the molecular hybridization instrument and the electron fluorescence microscope; and the terminal software analysis system analyzes the output information of the electron fluorescence microscope. The invention can realize full automation and intellectualization of sampling, processing, hybridizing, observing and outputting detection analysis reports, can quantitatively and visually monitor microbial community structure and spatial distribution, carries out multi-level, multi-level and multi-aspect comprehensive detection on target microorganisms according to detection parameters and multivariate knowledge data, and obtains the detection analysis reports within 8-20 hours. The invention has good market prospect and application prospect.
Description
Technical field
The present invention relates to a kind of microorganism gene detection analysis instrument, specifically a kind of to examine for environmental microorganism gene
Survey analyzer.
Background technology
Worldwide, the crude oil of subsurface deposit 30%～40% can only be produced with conventional oil recovery technique.How to improve
Recovery ratio, produces more remaining oils from underground, the problem that always world many countries are constantly studied for many years.
It is the utilization after traditional methods such as heating power drive, chemical flooding, polymer flooding that microorganism improves oil recovery factor
The beneficial activity of microorganism and metabolite are improving a comprehensive oil recovery technique of oil recovery factor.
Environmental problem has become the subject matter that China faces at this stage, and a little fields of here are required at microorganism participation
Reason, then the separation identification to microorganism has the problem for becoming primary, and it is art technology to filter out high-quality and efficient microorganism
The key that personnel explore.
Traditional microbiological oil recovery bacterial strain screening relies on reagent bottle detection, culture, by empirically determined solution, it is impossible to examine
Actual microbe species are surveyed, Parallel testing result difference is big；And, objective microbe can only be carried out single-stage not, single level, list
The analysis of aspect, time-consuming.
It is how quick, accurately, science and intuitively monitor and analysis environments microorganism becomes the focus of this area research.
The content of the invention
Present invention aim to address problem of the prior art, and propose one kind accurately can recognize, quick detection, science
The microorganism gene detection analysis instrument of analysis microorganism situation.
For achieving the above object, the present invention takes technical scheme below：
A kind of microorganism gene detection analysis instrument, it is characterised in that the analyzer includes A storehouses and B storehouses；
Terminal control room control vortex DL device, thermostat water bath, high speed freezing centrifuge and the first sample in the A storehouses
Operating room；The vortex DL device, thermostat water bath, high speed freezing centrifuge and the first sample operation room are respective defeated by which
Enter end and output end is connected in turn；
The input of the second sample operation room in the B storehouses is connected with the output end of the first sample operation room；Master control
Room controls the second sample operation room, Hybridization Oven and electronic fluorescent microscope；The second sample operation room, Hybridization Oven
It is connected in turn by its respective input and output end with electronic fluorescent microscope；
Also include terminal software analysis system to analyze electronic fluorescent microscope output information.
A kind of above-mentioned microorganism gene detection analysis instrument, wherein the A storehouses also include the first observation ward, for monitoring
State the processing procedure of vortex DL device, thermostat water bath, high speed freezing centrifuge and the first sample operation room.
A kind of above-mentioned microorganism gene detection analysis instrument, wherein between the vortex DL device and thermostat water bath, it is permanent
The first sampling is equipped between warm water bath and high speed freezing centrifuge, between high speed freezing centrifuge and the first sample operation room
System.
A kind of above-mentioned microorganism gene detection analysis instrument, wherein, the B storehouses also include the second observation ward, for monitoring
Second sample operation room, Hybridization Oven and the microscopical processing procedure of electronic fluorescent.
A kind of above-mentioned microorganism gene detection analysis instrument, wherein, the second sample operation room and Hybridization Oven it
Between, the second sampler is equipped between Hybridization Oven and electronic fluorescent microscope.
A kind of above-mentioned microorganism gene detection analysis instrument, wherein, the terminal software analysis system includes central control
System, intelligent checking system, analysis system, data management system, networking management system and expert's prescription system.
A kind of microorganism gene detection analysis instrument purposes of the present invention is recommended as follows：
1st, return water is sterilized
2nd, polymer displacement of reservoir oil
3rd, microbe oil production
4th, water-oil separating
5th, microorganism wax removal
6th, microorganism de-plugging
Advantages of the present invention and beneficial effect are：
The present invention is applied to accurate identification, quick according to genetic fingerprints recognition principle and FISH principle
Detection, scientific analysis microorganism situation.The present invention can accurately, quantitatively, intuitively monitor biological community structure and spatial distribution,
According to detection parameter and polynary knowledge data, multi-level, multi-level, many-sided comprehensive analysis is carried out to objective microbe, detect,
Quickly go out detection and analysis report.
The present invention can be detected for microorganism, especially for the microbial ecosystem of various environment, in the present invention
Particular use of the present invention, but the not restriction to range of application of the present invention are introduced in content by taking petroleum microorganism as an example.
Description of the drawings
Fig. 1 is the principle schematic diagram. of the present invention.
Specific embodiment
As shown in figure 1, a kind of microorganism gene detection analysis instrument, the analyzer includes A storehouses and B storehouses；
The control of terminal control room 7 vortex DL device 2, thermostat water bath 3, high speed freezing centrifuge 4 and first in the A storehouses
Sample operation room 5；Vortex DL device 2, thermostat water bath 3, high speed freezing centrifuge 4 and the first sample operation room 5 are each by which
From input and output end be connected in turn；
The input of the second sample operation room 8 in the B storehouses is connected with the output end of the first sample operation room 5；Master Control Room
13 the second sample operation rooms 8 of control, Hybridization Oven 10 and electronic fluorescent microscope 11；Second sample operation room 8, molecule hybridizes
Instrument 10 and electronic fluorescent microscope 11 are connected in turn by its respective input and output end；
Also include terminal software analysis system 14 to analyze 11 output information of electronic fluorescent microscope.
A storehouses also include the first observation ward 1, for monitoring the vortex DL device 2, thermostat water bath 3, high speed refrigerated centrifuge
Machine 4 and the processing procedure of the first sample operation room 5.
Between the vortex DL device 2 and thermostat water bath 3, between thermostat water bath 3 and high speed freezing centrifuge 4, it is high
The first sampler 6 is equipped between fast refrigerated centrifuge 4 and the first sample operation room 5.
B storehouses also include the second observation ward 9, aobvious for monitoring the second sample operation room 8, Hybridization Oven 10 and electronic fluorescent
The processing procedure of micro mirror 11.Between second sample operation room 8 and Hybridization Oven 10, Hybridization Oven 10 it is micro- with electronic fluorescent
The second sampler 12 is equipped between mirror 11.
The terminal software analysis system 14 includes central control system, intelligent checking system, analysis analysis system, data
Management system, networking management system and expert's prescription system.
The specifically used example of the present invention is as follows：
As shown in figure 1, weighing 1.5g oil reservoir sediment samples, 30mL is added to remove humic acid buffer solution (100mmol/
LTris-HClpH10.0,100mmol/lNa4P2O7100mmol/LNa2EDTA, 1.0%PVP, 100mmol/LNaCl,
0.05%TritonX-100), be vortexed on vortex DL device 2 3min, and be vortexed after 60 DEG C of water-bath 5min on thermostat water bath 3 1min,
Centrifugal rotational speed, temperature and centrifugation time required for arranging on high speed freezing centrifuge 4, centrifugation discard supernatant after terminating.Weight
Multiple said process 5-7 time, clarifying to sample treatment buffer (removing humic acid buffer solution), effectively removing affects the miscellaneous of detection
Matter.
Oil reservoir water sample (0.5L) collects microbial cell after concentration, and removing with said method process affects detection
Impurity.Sample after process completes sample and fixes and strengthen penetrating process through the first sample operation room 5, thin by microorganism
Cell wall, membrane passage intensive treatment fixed microbial cell in sample later.First observation ward 1 and terminal control room 7
Joint real-time monitoring, the whole flow process of control sample pretreatment.
Sample after above-mentioned A storehouses are processed is continued with gene probe kit through B storehouses the second sample operation room 8,
Hybridized with objective microbe small subunit ribosome RNA (SSUrRNA) specific fragment by probe in Hybridization Oven 10,
Probe and fluorescence labeling is made to stay in microbial cell.Subsequently fluorescence signal is excited under electronic fluorescent microscope 11, by signal
Transmit to Master Control Room 13, (include central control system, intelligent checking system, analysis system, number in terminal software analysis system 14
According to management system, networking management system and expert's prescription system) under automatically micro- scanning, carry out microorganism detection and fluorescence signal
Collect.The information such as the population structure and spatial distribution of objective microbe are obtained so as to directly perceived, accurate, through information fusion, terminal
Software analysis system provides " making a definite diagnosis " or " doubtful " analysis report, can various shapes such as real time print, imprinting, broadcasting, data transfer
Formula is exported.
The work of the present invention is further illustrated in this specific embodiment so that petroleum deposits extract identification microorganism as an example
Process and principle, microorganism gene detection analysis instrument of the present invention may not only be applied to petroleum microorganism field, can also be wide
The necks such as the general analysis for being applied to environmental microorganism, such as wastewater and sewage process, industrial tail gas Solid state fermentation, rivers and lakes pollution process
Domain, accurately can recognize, quick detection, scientific analysis microorganism situation, instruct environmental protection or relevant departments correctly select Environment control
Pollution microorganism used therefor and method.
Claims (6)
1. a kind of microorganism gene detection analysis instrument, it is characterised in that the analyzer includes A storehouses and B storehouses；
Terminal control room control vortex DL device, thermostat water bath, high speed freezing centrifuge and the first sample operation in the A storehouses
Room；The vortex DL device, thermostat water bath, high speed freezing centrifuge and the first sample operation room pass through its respective input
It is connected in turn with output end；
The input of the second sample operation room in the B storehouses is connected with the output end of the first sample operation room；Master Control Room control
Make the second sample operation room, Hybridization Oven and electronic fluorescent microscope；The second sample operation room, Hybridization Oven and electricity
Sub- fluorescence microscope is connected in turn by its respective input and output end；
Also include terminal software analysis system to analyze electronic fluorescent microscope output information.
2. a kind of microorganism gene detection analysis instrument according to claim 1, it is characterised in that the A storehouses also include the
One observation ward, for monitoring the place of the vortex DL device, thermostat water bath, high speed freezing centrifuge and the first sample operation room
Reason process.
3. a kind of microorganism gene detection analysis instrument according to claim 1 and 2, it is characterised in that the vortex DL
Between device and thermostat water bath, between thermostat water bath and high speed freezing centrifuge, high speed freezing centrifuge and the first sample behaviour
Make between room, to be equipped with the first sampler.
4. a kind of microorganism gene detection analysis instrument according to claim 3, it is characterised in that the B storehouses also include the
Two observation ward, for monitoring the second sample operation room, Hybridization Oven and the microscopical processing procedure of electronic fluorescent.
5. a kind of microorganism gene detection analysis instrument according to claim 1 or 4, it is characterised in that second sample
The second sampler is equipped between operating room and Hybridization Oven, between Hybridization Oven and electronic fluorescent microscope.
6. a kind of microorganism gene detection analysis instrument according to claim 5, it is characterised in that the terminal software analysis
System includes central control system, intelligent checking system, analysis system, data management system, networking management system and expert
Method, system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611087661.8A CN106554919A (en) | 2016-12-01 | 2016-12-01 | Microorganism gene detection analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611087661.8A CN106554919A (en) | 2016-12-01 | 2016-12-01 | Microorganism gene detection analyzer |
Publications (1)
| Publication Number | Publication Date |
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| CN106554919A true CN106554919A (en) | 2017-04-05 |
Family
ID=58445477
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201611087661.8A Pending CN106554919A (en) | 2016-12-01 | 2016-12-01 | Microorganism gene detection analyzer |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101403724A (en) * | 2008-10-08 | 2009-04-08 | 湖南大学 | Instrument and reagent for fast detection of microbe in blood specimen, and preparation method thereof |
| CN201587942U (en) * | 2009-12-21 | 2010-09-22 | 路域生态工程有限公司 | Microorganism gene detection analysis diagnostic apparatus |
| CN103091261A (en) * | 2011-11-04 | 2013-05-08 | 北京倍肯恒业科技发展有限责任公司 | Digitalized food safety rapid detecting system |
-
2016
- 2016-12-01 CN CN201611087661.8A patent/CN106554919A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101403724A (en) * | 2008-10-08 | 2009-04-08 | 湖南大学 | Instrument and reagent for fast detection of microbe in blood specimen, and preparation method thereof |
| CN201587942U (en) * | 2009-12-21 | 2010-09-22 | 路域生态工程有限公司 | Microorganism gene detection analysis diagnostic apparatus |
| CN103091261A (en) * | 2011-11-04 | 2013-05-08 | 北京倍肯恒业科技发展有限责任公司 | Digitalized food safety rapid detecting system |
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Application publication date: 20170405 |
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