CN106551054A - Folium Mali Hupehensis black tea and its preparation technology and purposes - Google Patents
Folium Mali Hupehensis black tea and its preparation technology and purposes Download PDFInfo
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- CN106551054A CN106551054A CN201610980118.4A CN201610980118A CN106551054A CN 106551054 A CN106551054 A CN 106551054A CN 201610980118 A CN201610980118 A CN 201610980118A CN 106551054 A CN106551054 A CN 106551054A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/34—Tea substitutes, e.g. matè; Extracts or infusions thereof
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Abstract
The invention discloses a kind of Folium Mali Hupehensis black tea preparation technology, comprises the steps：Step one, Folium Mali Hupehensis are taken as Folium Camelliae sinensis, and withered；Step 2, will wither after Folium Mali Hupehensis tea leaf fermentation, in fermentation, individually or collectively fermented using Monascus ruber bacterium, three kinds of strains of yeast and mucormycosiss, wherein, bacterium solution inoculum concentration in fermentation or co-fermentation is the 1%～6% of Folium Mali Hupehensis quality respectively, fermentation liquid water content 40%～60%, initial pH5～6 of fermentation liquid, when fermenting respectively, the Folium Camelliae sinensis after three kinds of strain fermentations are mixed；Step 3, the Folium Camelliae sinensis after fermentation are kneaded；Step 4, the Folium Camelliae sinensis after kneading are dried, obtain Folium Mali Hupehensis black tea.The present invention changes black tea processing natural fermentation to mix bacterium control fermentation, defines the sweet and pure certain quality of soup red autumnal leaves fragrance, and has loosening bowel to relieve constipation, diuresis New function.Lipid-loweringing, fat-reducing, eliminating toxin and beautifying the skin effect can reach by loosening bowel to relieve constipation and diuresis.
Description
Technical field
The invention belongs to Folium Camelliae sinensis preparing technical field, is related to a kind of Folium Mali Hupehensis black tea and its preparation technology and purposes.
Background technology
Black tea is one of six big teas, and China is the cradle of black tea manufacture, has just invented black tea early in 16 end of the centurys.
Black Tea red autumnal leaves fragrance is sweet and pure, in addition to possessing the quality flavour of uniqueness, also with many functions such as nutrition and pharmacology,
Have outstanding performance in six big teas, therefore black tea is elected one of teas for drinking for optimum.
China is one of most abundant country of tea tree planting resource, and conventional black resource is including Folium Mali Hupehensis.Folium Mali Hupehensis
(Malus hupehensis (Pamp.) Rehd.) is Rosaceae Malus, scientific name Hubei Chinese flowering crabapple, calls premium tea, is me
The endemic plant of state.Folium Mali Hupehensis tender leaf drinks oneself as Folium Camelliae sinensis history in thousand, is Chongqing east, west place in Hubei, Wuhan, Changsha, Chongqing etc.
The refreshment drink that big and medium-sized cities summer widely drinks, being commonly called as black tea.The main component phlorhizin of Folium Mali Hupehensis belongs to dihydrochalcone
Glycosides, compared with other flavonoids, is referred to as " minority flavonoid ".With a long history due to drinking, Ministry of Public Health 2014 the 20th is public
It is new raw-food material to accuse approval Hubei Chinese flowering crabapple (Folium Mali Hupehensis) leaf.Folium Mali Hupehensis conventional machining process is dried for fresh tea picking.
The content of the invention
It is an object of the invention to solve at least the above and/or defect, and provide at least will be described later excellent
Point.
It is a still further object of the present invention to provide a kind of preparation technology of Folium Mali Hupehensis black tea.
A further object of the present invention is to provide a kind of Folium Mali Hupehensis black tea.
The present invention separately has a purpose to be to provide described Folium Mali Hupehensis black tea is used for the use of organism diuresis and loosening bowel to relieve constipation
On the way.
For this purpose, the technical scheme that the present invention is provided is：
A kind of Folium Mali Hupehensis black tea preparation technology, comprises the steps：
Step one, Folium Mali Hupehensis are taken as Folium Camelliae sinensis, and withered；
Step 2, will wither after Folium Mali Hupehensis tea leaf fermentation, in fermentation, using Monascus ruber bacterium, yeast and mucormycosiss three
Kind of strain is individually or collectively fermented, wherein, the bacterium solution inoculum concentration in fermentation or co-fermentation is Folium Mali Hupehensis quality respectively
1%～6%, fermentation liquid water content 40%～60%, initial pH5～6 of fermentation liquid, when fermenting respectively, by three kinds of strain fermentations
Folium Camelliae sinensis mixing afterwards；
Step 3, the Folium Camelliae sinensis after fermentation are kneaded；
Step 4, the Folium Camelliae sinensis after kneading are dried, obtain Folium Mali Hupehensis black tea.
Preferably, in described Folium Mali Hupehensis black tea preparation technology, in the step 2, the fermentation condition is temperature 20
～35 DEG C of 3～12d of bottom fermentation.
Preferably, in described Folium Mali Hupehensis black tea preparation technology, in the step 2, the fermentation condition is sent out for alternating temperature
Ferment, fermentation temperature are to gradually rise to 30 DEG C from 20, then are naturally cooling to 20 DEG C, change thermoperiod into 12h, fermentation time be 3～
12d。
Preferably, in described Folium Mali Hupehensis black tea preparation technology, in the step 3, the Folium Camelliae sinensis after fermentation are rubbed
The process of sth. made by twisting includes：
According to light-heavy-light pressurization principle, the pressurized treatments of 10min-30min-10min are carried out, 30-60min is kneaded.
Preferably, in described Folium Mali Hupehensis black tea preparation technology, in the step 4, the Folium Camelliae sinensis after kneading are carried out
Drying adopts stage drying, including：It is dried 40-60min first at temperature 40-50 DEG C, is then dried at 100 DEG C of temperature
30-40min, is dried to moisture in Folium Camelliae sinensis at 80 DEG C of end temperature and is less than 10%.
Preferably, in described Folium Mali Hupehensis black tea preparation technology, in the step one, the side that Folium Mali Hupehensis blade is withered
Method includes：In 20 DEG C -25 DEG C of temperature, humidity 50%-85% and the 10～18h that withers under the conditions of stacking 5～20cm of thickness.
Preferably, in described Folium Mali Hupehensis black tea preparation technology, in the step 2, in fermentation, the Monascus ruber bacterium,
The mass ratio of yeast and mucoraceous bacterium solution inoculum concentration is 1:1：1.
A kind of Folium Mali Hupehensis black tea, the Folium Mali Hupehensis black tea are prepared by such as arbitrary described technique.
Described Folium Mali Hupehensis black tea is used for the purposes of organism diuresis and loosening bowel to relieve constipation.
The present invention at least includes following beneficial effect：
The present invention changes black tea processing natural fermentation to mix bacterium control fermentation, and red koji fermentation can strengthen the intestine moistening of Caulis et folium euphorbiae milii black tea and lead to
Just act on, Mucor fermentation can strengthen Caulis et folium euphorbiae milii black tea diuresis, yeast fermentation can eliminate astringent taste, increase fragrance, improving quality, shape
Into the sweet and pure certain quality of soup red autumnal leaves fragrance, and there is loosening bowel to relieve constipation, diuresis New function.It is reachable by loosening bowel to relieve constipation and diuresis
To lipid-loweringing, fat-reducing, eliminating toxin and beautifying the skin effect.Also, the processing technique of conventional black is made adjustment, is allowed to be suitable to process tea sea
Chinese bush cherry.
Part is embodied by the further advantage of the present invention, target and feature by description below, and part will also be by originally
The research of invention and practice and be understood by the person skilled in the art.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to description
Word can be implemented according to this.
It should be appreciated that it is used herein such as " have ", "comprising" and " including " term do not allot one or many
The presence or addition of individual other elements or its combination.
The present invention provides a kind of Folium Mali Hupehensis black tea preparation technology, comprises the steps：
Step one, Folium Mali Hupehensis are taken as Folium Camelliae sinensis, and withered；
Step 2, will wither after Folium Mali Hupehensis tea leaf fermentation, in fermentation, using Monascus ruber bacterium, yeast and mucormycosiss three
Kind of strain is individually or collectively fermented, wherein, the bacterium solution inoculum concentration in fermentation or co-fermentation is Folium Mali Hupehensis quality respectively
1%～6%, fermentation liquid water content 40%～60%, initial pH5～6 of fermentation liquid, when fermenting respectively, by three kinds of strain fermentations
Folium Camelliae sinensis mixing afterwards；Stop after the visible fermented hypha of whole Folium Camelliae sinensis.
Step 3, the Folium Camelliae sinensis after fermentation are kneaded；
Step 4, the Folium Camelliae sinensis after kneading are dried, obtain Folium Mali Hupehensis black tea.
Traditionally black tea processing technique mainly includes four basic working procedures of withering-knead-ferment-be dried, root of the present invention
According to material ins and outs, Folium Mali Hupehensis are processed, by process modifications to wither-fermenting-knead-be dried, wherein fermentation is most important
Innovation operation, change black tea processing natural fermentation for mixed bacterium control fermentation, define the sweet and pure certain quality of soup red autumnal leaves fragrance,
And there is loosening bowel to relieve constipation, diuresis New function.Lipid-loweringing, fat-reducing, eliminating toxin and beautifying the skin effect can reach by loosening bowel to relieve constipation and diuresis.
In one of embodiment of the present invention, preferably, in described Folium Mali Hupehensis black tea preparation technology, the step
In rapid two, the fermentation condition is 20～35 DEG C of 3～12d of bottom fermentation of temperature.
In one of embodiment of the present invention, preferably, in the step 2, the fermentation condition is sent out for alternating temperature
Ferment, fermentation temperature are to gradually rise to 30 DEG C from 20, then are naturally cooling to 20 DEG C, change thermoperiod into 12h, fermentation time be 3～
12d。
In one of embodiment of the present invention, preferably, in the step 3, the Folium Camelliae sinensis after fermentation are rubbed
The process of sth. made by twisting includes：Kneaded using kneading machines such as 6CR-25 types, according to light-heavy-light pressurization principle, carry out 10min-30min-
The pressurized treatments of 10min, knead 30-60min.Knead tea bar rope is tight volume, it is hand-tight hold to knead leaf and occur tea juice between webs be
Preferably.
In one of embodiment of the present invention, preferably, in the step 4, the Folium Camelliae sinensis after kneading are carried out
Drying adopts stage drying, including：It is dried 40-60min first at temperature 40-50 DEG C, is then dried at 100 DEG C of temperature
30-40min, is dried to moisture in Folium Camelliae sinensis at 80 DEG C of end temperature and is less than 10%.Be dried to Folium Camelliae sinensis handle light sth. made by twisting it is unqualified i.e.
Can.
In one of embodiment of the present invention, preferably, in the step one, the side that Folium Mali Hupehensis blade is withered
Method includes：In 20 DEG C -25 DEG C of temperature, humidity 50%-85% and the 10～18h that withers under the conditions of stacking 5～20cm of thickness.Withering leaf
State reaches profile shrinkage, softness, and loosing one's grip can be slowly loose, and leaf color is dark green, sends out thoroughly delicate fragrance.
In one of embodiment of the present invention, preferably, in the step 2, in fermentation, the Monascus ruber bacterium,
The mass ratio of yeast and mucoraceous bacterium solution inoculum concentration is 1:1：1.
The present invention also provides a kind of Folium Mali Hupehensis black tea, and the Folium Mali Hupehensis black tea is prepared by arbitrary described technique.
The present invention also provides the purposes that described Folium Mali Hupehensis black tea is used for organism diuresis and loosening bowel to relieve constipation.
Embodiment 1
A kind of Folium Mali Hupehensis black tea preparation technology, comprises the steps：
Step one, Folium Mali Hupehensis are taken as Folium Camelliae sinensis, and withered；Withered using Indoor Natural, temperature is put into after harvesting and is existed
20 DEG C, humidity 50% is clean indoor, stacks thickness 5cm and is withered, and wither 10h, and leaf state of withering reaches profile shrinkage, soft
Soft, loosing one's grip can be slowly loose, and leaf color is dark green, sends out thoroughly delicate fragrance.
Step 2, will wither after Folium Mali Hupehensis tea leaf fermentation, in fermentation, using Monascus ruber bacterium, yeast and mucormycosiss three
Plant strain to be fermented respectively, after fermentation, the Folium Camelliae sinensis after three kinds of strain fermentations are mixed；Wherein, the bacterium solution in fermenting respectively connects
The amount of kind is the 1% of Folium Mali Hupehensis quality, and fermentation liquid water content 40%, the initial pH5 of fermentation liquid, fermentation condition are issued for 20 DEG C of temperature
Ferment 12d.
Step 3, the Folium Camelliae sinensis after fermentation are kneaded, the kneading machine such as 6CR-25 types is kneaded, according to the pressurization of light-weight-light
Principle, carries out, under 10min-30min-10min pressurized treatments, kneading 30min, kneads tea bar rope is tight volume, and hand-tight holding kneads leaf
Occur tea juice between webs to be advisable.
Step 4, the Folium Camelliae sinensis after kneading are dried, 30min is dried at temperature 40-50 DEG C first, then in temperature
30min is dried at 100 DEG C of degree, is dried at 80 DEG C of end temperature to moisture in Folium Camelliae sinensis and is less than 10%, Folium Camelliae sinensis handle light sth. made by twisting it is broken
End.Obtain Folium Mali Hupehensis black tea.
Obtained Folium Mali Hupehensis black tea is used for the purposes of organism diuresis and loosening bowel to relieve constipation.
Embodiment 2
A kind of Folium Mali Hupehensis black tea preparation technology, comprises the steps：
Step one, Folium Mali Hupehensis are taken as Folium Camelliae sinensis, and withered；Withered using Indoor Natural, temperature is put into after harvesting and is existed
25 DEG C, humidity 85% is clean indoor, stacks thickness 20cm and is withered, and wither 18h, and leaf state of withering reaches profile shrinkage, soft
Soft, loosing one's grip can be slowly loose, and leaf color is dark green, sends out thoroughly delicate fragrance.
Step 2, will wither after Folium Mali Hupehensis tea leaf fermentation, in fermentation, using Monascus ruber bacterium, yeast and mucormycosiss three
Plant strain to be fermented respectively, after fermentation, the Folium Camelliae sinensis after three kinds of strain fermentations are mixed；Wherein, the bacterium solution in fermenting respectively connects
The amount of kind is the 6% of Folium Mali Hupehensis quality, and fermentation liquid water content 60%, the initial pH6 of fermentation liquid, fermentation condition are issued for 35 DEG C of temperature
Ferment 3d.
Step 3, the Folium Camelliae sinensis after fermentation are kneaded, the kneading machine such as 6CR-25 types is kneaded, according to the pressurization of light-weight-light
Principle, carries out, under 10min-30min-10min pressurized treatments, kneading 60min, kneads tea bar rope is tight volume, and hand-tight holding kneads leaf
Occur tea juice between webs to be advisable.
Step 4, the Folium Camelliae sinensis after kneading are dried, 40min is dried under temperature 50 C first, then in temperature
40min is dried at 100 DEG C, is dried at 80 DEG C of end temperature to moisture in Folium Camelliae sinensis and is less than 10%, Folium Camelliae sinensis handle light sth. made by twisting it is unqualified
.Obtain Folium Mali Hupehensis black tea.
Obtained Folium Mali Hupehensis black tea is used for the purposes of organism diuresis and loosening bowel to relieve constipation.
Embodiment 3
A kind of Folium Mali Hupehensis black tea preparation technology, comprises the steps：
Step one, Folium Mali Hupehensis are taken as Folium Camelliae sinensis, and withered；Withered using Indoor Natural, temperature is put into after harvesting and is existed
23 DEG C, humidity 68% is clean indoor, stacks thickness 13cm and is withered, and wither 14h, and leaf state of withering reaches profile shrinkage, soft
Soft, loosing one's grip can be slowly loose, and leaf color is dark green, sends out thoroughly delicate fragrance.
Step 2, will wither after Folium Mali Hupehensis tea leaf fermentation, in fermentation, using Monascus ruber bacterium, yeast and mucormycosiss three
Plant strain to be fermented jointly, wherein, bacterium solution inoculum concentration is the 3% of Folium Mali Hupehensis quality, Monascus ruber bacterium, yeast and mucormycosiss
Bacterium solution inoculum concentration in mass ratio be 1:1：1, fermentation liquid water content 50%, the initial pH5.5 of fermentation liquid, when fermenting respectively,
Folium Camelliae sinensis after three kinds of strain fermentations are mixed；Fermentation condition is temperature-variable fermentation, and fermentation temperature is to gradually rise to 30 DEG C from 20, then
20 DEG C are naturally cooling to, into 12h, fermentation time is 3～12d to change thermoperiod.
Step 3, the Folium Camelliae sinensis after fermentation are kneaded, the kneading machine such as 6CR-25 types is kneaded, according to the pressurization of light-weight-light
Principle, carries out, under 10min-30min-10min pressurized treatments, kneading 45min, kneads tea bar rope is tight volume, and hand-tight holding kneads leaf
Occur tea juice between webs to be advisable.
Step 4, the Folium Camelliae sinensis after kneading are dried, 50min is dried under temperature 45 C first, then in temperature
35min is dried at 100 DEG C, is dried at 80 DEG C of end temperature to moisture in Folium Camelliae sinensis and is less than 10%, Folium Camelliae sinensis handle light sth. made by twisting it is unqualified
.Obtain Folium Mali Hupehensis black tea.
Obtained Folium Mali Hupehensis black tea is used for the purposes of organism diuresis and loosening bowel to relieve constipation.
Embodiment 4
First, Folium Mali Hupehensis black tea preparation technology
1st, experiment material and reagent
1.1 experimental strains and Folium Camelliae sinensis
Monas cuspurpureus Went Monascus purpureus, yeast Saccharomyces cerevisiae Mucor (Actinomucor
elegans).Above strain is available to belong to strain not of the same race together.Folium Mali Hupehensis leaf：(Malus hupehensis (Pamp.) Rehd.'s
Tender leaf.There is provided by Natural products research and using key lab of Hubei Province (SanXia University).
1.2 experiment reagent
Dehydrated alcohol, glucose, peptone, KH2PO4MgS04.7H20th, Calcium Carbonate, PDA culture medium, all reagents are
Analysis is pure.
1.3 experimental apparatus
6CR-45 type tea twisting machines, Zhejiang Green Peak Machinery Co., Ltd., hot constant temperature blast drying oven, Shanghai one are permanent, vertical
Formula pressure steam sterilization boiler, Japanese HIRAYAMA companies, ultra-pure water instrument, Ai Kepu scientific ＆ technical corporation, superclean bench, Suzhou purification
Instrument factory, electronic balance, Shanghai Min Qiao precision instruments company limited, electro-heating standing-temperature cultivator, Wuhan elite Science ＆ Teaching Instrument are limited
Company, digital thermostat water-bath, Changzhou Zhi Borui instrument manufacturings company limited, ultrasonic cleaner, city of Kunshan's ultrasonic instrument have
Limit company etc..
1.4 experimental technique and result
1.4.1 wither：Withered using Indoor Natural, temperature is put into after harvesting at 20 DEG C～25 DEG C, humidity 50%～85%
It is clean indoor, stack 5～20cm of thickness and withered, wither 10～18h, and leaf state of withering reaches profile shrinkage, softness, looses one's grip
Can be slowly loose, leaf color is dark green, sends out thoroughly delicate fragrance.
1.4.2 fermentation：Fermented using different bacterium respectively, then mixed, or directly mixed fungus fermentation mode.
1.4.2.1 culture medium is prepared and spawn culture
Strain inclined plane culture medium：Appropriate PDA agar culture mediums are weighed, adds appropriate aquae destillata heated and stirred dissolving to be distributed into
In test tube, 120 DEG C, 20min sterilizings are placed inclined-plane (bevel altitude is about test tube height 1/3rd) and are cooled to solidification.
Seed liquid culture medium：Glucose 30g/L, MgSO4·7H2O 1g/L, KH2PO41g/L, peptone 30g/L, add
Appropriate aquae destillata, heated and stirred dissolving, is sub-packed in triangular flask, and 100-500ml is accumulated per bottling liquid, and 120 DEG C, 20min sterilizes,
Cooling is standby.
Actication of culture：The decentralization of slant preservation strain room temperature is postponed, and is accessed in the new slant medium prepared, in constant temperature culture
In case 20～30 DEG C stand activation 3～7 days it is stand-by.
Seed liquor culture：Liquid culture medium is planted by what activated spawn access was newly prepared, loaded in triangular flask, 20-30 DEG C quiet
Put or 50～200rpm under the conditions of culture 3～9 days it is stand-by.
Fermentation culture：Seed liquor culture is mixed with the Folium Camelliae sinensis for withering, by 1～6% addition single bacterium or same of tea amount
When add various bacterium.Control water content 40～60%, best 50%, 5～6,20～35 DEG C of 3～12d of fermentation of initial pH.With modeling
Pocket is filled, or Polypropylence Sheet compacting, natural fermentation, or fermentation tank temperature-variable fermentation after pile, 20～30 DEG C of temperature, becomes thermoperiod 12h.
Stop after the visible fermented hypha of whole Folium Camelliae sinensis.
1.4.3 knead：The kneading machines such as 6CR-25 types are kneaded, and according to light-heavy-light pressurization principle, carry out 10min-
Under 30min-10min pressurized treatments, 30～60min is kneaded, knead tea bar rope is tight volume, hand-tight holding is kneaded leaf and occurred between webs
Tea juice is advisable.
It 1.4.4 is dried：40-50 DEG C of 40～60min of drying of stage drying, 100 DEG C of 30～40min of drying, then do for 80 DEG C
It is dry to Folium Camelliae sinensis handle light sth. made by twisting it is unqualified.
2nd, Folium Mali Hupehensis black tea functional experiment
1 material
1.1 medicine and reagent
1.1.1 the preparation of test sample
Folium Mali Hupehensis black tea is provided by Natural products research and using key lab of Hubei Province (SanXia University).It is respectively former
Tea, Monas cuspurpureus Went, yeast, Mucor ferment and three bacterium mixed fermentation tea samples respectively.
Title tea sample is appropriate respectively, plus 10 times of water, and 80 DEG C of warm water are extracted 2 times, each 1h, merges lixiviating solution and filters, concentrates
It is to the suitable 0.1g Folium Camelliae sinensis of 1ml, standby.
1.1.2 reagent
Fructus Cannabis Bolus (specification：30g/ bottles, Hubei promise are won victory pharmaceutical Co. Ltd, lot number：Z42020417, is made into before use
The suspension of 0.3g/ml).Hydrochlorothiazide (Changzhou Pharmaceutical Factory Co., Ltd, Chinese medicines quasi-word H32021683).Compound diphenoxylate
Piece (Shandong Kernel and Hall Pharmaceutical Industry Co., Ltd., Chinese medicines quasi-word H37021525 make suspension by 1mg/10ml).
1.2 animal
Kunming mouse, male, body weight 18-22g are provided by SanXia University's animal experimental center, animal productiong licensing
Number it is：SYXK (Hubei Province) 2011-0012.In constant temperature (24 ± 2 DEG C), periodicity of illumination 12h：Test after raising in 12h environment 1 week, it is real
Test animal facility and use credit number：SYXK (Hubei Province) 2011-0061.
1.3 instrument
HG-100A flame photometers, Beijing profit and Science ＆ Technology Center's production, SP-50lc type semi-automatic biochemical analyzers, China
Taiwan Yuan Sheng companies, electronic balance, Shanghai Min Qiao precision instruments company limited
2. method and result
The impact of 2.1 pairs of mice voided volume and urine intermediate ion concentration
2.1.1 diuresis experiment：70 mices are randomly divided into into 7 groups, respectively Normal group (gavage normal saline), sun
Property medicine matched group (Hydrochioro group), Folium Mali Hupehensis original tea group, red koji fermentation group, yeast fermentation group, Mucor fermentation group, mixed fermentation
Group.Before experiment, fasting feedwater 24h, fasting for solids and liquids during experiment, light to press mice hypogastric region to drain remaining urine, every mouse peritoneal injection
Normal saline 1ml is used as Water l oad.Above each group presses 0.1ml/10g gavages, records administration time, after administration is put into mice
In metabolic cage, 1 urine volume is collected per 2h, 4h urine volume is collected.(2) potassium, sodium, the measure of chlorine ion concentration are urinated：Essence takes 5 μ l of urine sample,
Detection potassium, Na ion concentration are carried out with flame photometer；Essence takes 1 μ l of urine sample, detects chloride ion content with biochemistry analyzer.
2.1.2 statistical method
So data are compared between group and checked using t using the process of SPSS13.0 statistical softwares, measurement data is usedTable
Show.
2.1.3 experimental result
Hydrochioro group, Caulis et folium euphorbiae milii leaf group and mixed fermentation group can increase the excretion of mice urine volume, with matched group than difference pole
Significantly (P<0.01), in urine, sodium, potassium, the discharge of chloride ion also roll up (P<0.05 or P<0.01)；Folium Mali Hupehensis original tea group, hair
Mould fermentation group can increase the excretion of urine volume, with matched group than significant difference (P<0.05), sodium, chloride ion in Folium Mali Hupehensis original tea group urine
Discharge increase (P<0.05 or P<0.01).
As a result show that Folium Mali Hupehensis have diuresis, fermentation can strengthen Folium Mali Hupehensis diuresis, and Mucor Fermentation is obvious.
1 each group mice voided volume of table and urine intermediate ion concentration compare
Note：Compare * P with Normal group<0.05, * * P<0.01
The impact of 2.2 pairs of ink progradations and feces
2.2.1 the defecation experiment of mice
Normal group and model control group give normal saline, and positive drug control group gives Fructus Cannabis Bolus suspension, remaining
Group gives correspondence reagent solution, and each group presses 0.1ml/10g gavages, and ad lib water inlet is fed 7 days laggard row indexs and determined.
Determine the one before group mice fasting and can't help water 24h, in addition to Normal group, remaining each group gavage compound diphenoxylate, normal control
Group gavage normal saline.After administration 30min, each group mice gives prepared Chinese ink respectively (containing 5% activated carbon powder, 10% Arabic
Natural gum) gavage.From the beginning of prepared Chinese ink is filled, record mice and arrange time of melena first, melena grain number and character in 4h after administration.
2.2.2 the intestinal motility experiment of mice
The same 2.2.1 of mice group, administering mode.After administration 30min, each group mice gives prepared Chinese ink respectively (containing 5% activated carbon
Powder, 10% Radix Acaciae senegalis) gavage, the mice of cervical dislocation execution immediately after 25min, whole section little of taking-up pylorus to ileocecus
Intestinal, calculates ink progradation, ink progradation (%)=ink propulsion length/total small intestinal length × 100%.
2.2.3 statistical method
So data are compared between group and checked using t using the process of SPSS13.0 statistical softwares, measurement data is usedTable
Show.
2.2.4 experimental result
Compared with Normal group, model control group mice row's melena time is obviously prolonged (P<0.05), defecation grain number is bright
It is aobvious to reduce (P<0.05), black surround is stiff, illustrates modeling success.Compared with model group, Fructus Cannabis Bolus group, Caulis et folium euphorbiae milii leaf group, mixed fermentation
Group, red koji fermentation group can promote ink progradation, shorten defecation time (P<0.05 or P<0.01),
As a result show that Folium Mali Hupehensis have the effect of loosening bowel to relieve constipation, fermentation can strengthen Folium Mali Hupehensis functions of loosening bowel relieving constipation, and Monas cuspurpureus Went is sent out
Ferment effect is obvious.
The ink progradation and defecation of 2 each group mice of table compare (N=10)
Note：Compare * P with model control group<0.05, * * P<0.01；Compare #P with Normal group<0.05
Module number described herein and treatment scale are the explanations for simplifying the present invention.Folium Mali Hupehensis to the present invention
The application of black tea preparation technology, modifications and variations will be readily apparent to persons skilled in the art.
Although embodiment of the present invention is disclosed as above, which is not restricted to listed by description and embodiment
With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily
Other modification is realized, therefore under the general concept limited without departing substantially from claim and equivalency range, the present invention is not limited
In specific details and shown here as the embodiment with description.
Claims (9)
1. a kind of Folium Mali Hupehensis black tea preparation technology, it is characterised in that comprise the steps：
Step one, Folium Mali Hupehensis are taken as Folium Camelliae sinensis, and withered；
Step 2, will wither after Folium Mali Hupehensis tea leaf fermentation, in fermentation, using Monascus ruber bacterium, three kinds of bacterium of yeast and mucormycosiss
Kind individually or collectively fermented, wherein, the bacterium solution inoculum concentration in fermentation or co-fermentation is the 1% of Folium Mali Hupehensis quality respectively
～6%, fermentation liquid water content 40%～60%, initial pH5～6 of fermentation liquid, when fermenting respectively, by the tea after three kinds of strain fermentations
Leaf mixes；
Step 3, the Folium Camelliae sinensis after fermentation are kneaded；
Step 4, the Folium Camelliae sinensis after kneading are dried, obtain Folium Mali Hupehensis black tea.
2. Folium Mali Hupehensis black tea preparation technology as claimed in claim 1, it is characterised in that in the step 2, the fermentation bar
Part is 20～35 DEG C of 3～12d of bottom fermentation of temperature.
3. Folium Mali Hupehensis black tea preparation technology as claimed in claim 1, it is characterised in that in the step 2, the fermentation bar
Part is temperature-variable fermentation, and fermentation temperature is to gradually rise to 30 DEG C from 20, then is naturally cooling to 20 DEG C, and change thermoperiod ferments into 12h
Time is 3～12d.
4. Folium Mali Hupehensis black tea preparation technology as claimed in claim 1, it is characterised in that in the step 3, after fermentation
The process kneaded by Folium Camelliae sinensis includes：
According to light-heavy-light pressurization principle, the pressurized treatments of 10min-30min-10min are carried out, 30-60min is kneaded.
5. Folium Mali Hupehensis black tea preparation technology as claimed in claim 1, it is characterised in that in the step 4, after kneading
Folium Camelliae sinensis be dried and adopt stage drying, including：40-60min is dried at temperature 40-50 DEG C first, then in temperature
30-40min is dried at 100 DEG C, is dried to moisture in Folium Camelliae sinensis at 80 DEG C of end temperature and is less than 10%.
6. Folium Mali Hupehensis black tea preparation technology as claimed in claim 1, it is characterised in that in the step one, by Folium Mali Hupehensis leaf
The method that piece withers includes：In 20 DEG C -25 DEG C of temperature, humidity 50%-85% and wither 10 under the conditions of stacking 5～20cm of thickness～
18h。
7. Folium Mali Hupehensis black tea preparation technology as claimed in claim 1, it is characterised in that in the step 2, in fermentation, described
The mass ratio of Monascus ruber bacterium, yeast and mucoraceous bacterium solution inoculum concentration is 1:1：1.
8. a kind of Folium Mali Hupehensis black tea, it is characterised in that technique of the Folium Mali Hupehensis black tea by as described in as arbitrary such as claim 1 to 7
Prepare.
9. the Folium Mali Hupehensis black tea described in claim 8 is used for the purposes of organism diuresis and loosening bowel to relieve constipation.
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| CN107197976A (en) * | 2017-07-18 | 2017-09-26 | 湖南农业大学 | Toringo compound bag-soak-tea and preparation method thereof |
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| CN108244283A (en) * | 2018-02-11 | 2018-07-06 | 平南县兴民龙藤茶业专业合作社 | A kind of preparation process of black tea |
| CN108272909A (en) * | 2018-01-31 | 2018-07-13 | 三峡大学 | A kind of preparation method and application of tea leaf fermentation product |
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| CN107197976A (en) * | 2017-07-18 | 2017-09-26 | 湖南农业大学 | Toringo compound bag-soak-tea and preparation method thereof |
| CN109691554A (en) * | 2017-10-23 | 2019-04-30 | 恩施硒海棠生物科技有限公司 | A kind of Hubei Chinese flowering crabapple black tea processing method and purposes |
| CN109769992A (en) * | 2017-11-10 | 2019-05-21 | 恩施硒海棠生物科技有限公司 | A kind of preparation method of Hubei Chinese flowering crabapple granulated red tea |
| CN107996945A (en) * | 2017-12-18 | 2018-05-08 | 三峡大学 | A kind of preparation method of Hubei Chinese flowering crabapple leaf raw material and its application on extraction phloridzin or phloretin |
| CN110089597A (en) * | 2018-01-30 | 2019-08-06 | 恩施硒海棠生物科技有限公司 | A kind of preparation method of mulberry leaf Chinese flowering crabapple tea |
| CN108272909A (en) * | 2018-01-31 | 2018-07-13 | 三峡大学 | A kind of preparation method and application of tea leaf fermentation product |
| CN108244283A (en) * | 2018-02-11 | 2018-07-06 | 平南县兴民龙藤茶业专业合作社 | A kind of preparation process of black tea |
| CN108936259A (en) * | 2018-07-27 | 2018-12-07 | 贵州省萌春茶业有限责任公司 | A kind of black tea wheat flour and preparation method thereof |
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Application publication date: 20170405 |