CN1065488A - A kind of quick and continuous brew does not contain the brewing method of carcinogenic beer - Google Patents

Abstract

A kind of quick and continuous brew does not contain the brewing method of carcinogenic beer, known beer with the Fructus Hordei Germinatus for the main raw material brew forms, it not only the production cycle long, the raw materials cost height, and contain carcinogenic substance N-nitrosodimethylamine, aflatoxin B1 in the beer, often drink unfavorable HUMAN HEALTH.The invention provides a kind of is raw material with a kind of in the corn fine powder of removing the embryo navel, the rice of removing cot, long-grained nonglutinous rice, glutinous rice, millet, sorghum rice, the seed of jog's tears, barley, wheat, highland barley, the oat or their mixture fine powder, and additional enzyme is produced the method for beer.Adopt the inventive method to brewage and do not contain carcinogens beer, technology is simple, and is with short production cycle, and the beer cost is low, has eradicated the carcinogenic substance in the beer fully.

Description

A kind of quick and continuous brew does not contain the brewing method of carcinogenic beer

A kind of quick and continuous brew does not contain the brewing method of carcinogenic beer, belongs to the application method class of the brewing method and the enzyme of beer.

Beer mainly is to be main raw material with the Fructus Hordei Germinatus now, forms through saccharification and fermentation.Used enzyme in the saccharifying is provided by Fructus Hordei Germinatus, as α-Dian Fenmei, and β-saccharifying enzyme, proteolytic enzyme etc.Along with the development of chemistry, medical science, the appearance of rigorous analysis instrument and application, will adopt Fructus Hordei Germinatus is all inherent shortcoming of main raw material beer brewing to disclose out.The one, the Hordeum low-yielding crop is adopted barley beer brewing waste arable land beyond doubt, and underproduction grain reduces economic benefit, and especially China's barley production is few, and wherein major part is a feed barley, and the special-purpose barley of beer just still less; The 2nd, run counter to biochemical basis: need during malt to wash, must discharge a large amount of moisture content again during oven dry, and add big water gaging during saccharification again again with the volume water logging.Require Fructus Hordei Germinatus to have enough enzyme activities during saccharification so that increasing supplementary product consumption reduces cost, and make a large amount of enzyme deactivations in malt drying, this obviously is again the waste of a kind of energy and enzyme; The 3rd, contain carcinogenic substance-N-nitrosodimethylamine in the Fructus Hordei Germinatus, and contain another kind of carcinogenic poisonous substance-aflatoxin B1 again in the beer of brewageing with this method, these two kinds of materials all belong to strong carcinogens, generally acknowledged by 6000 each carninomatosis expert of 65 countries already, therefore, drink Fructus Hordei Germinatus beer in a large number and will bring harm to HUMAN HEALTH.Disclose among the US-A-3081172 and a kind ofly added the method that animal ferment is produced syrup for beer with unmalted grain, the weak point of this method is: 1, the source of enzyme is an animal body, and consumption is big, can not reach mass industrialized production.2, with grain be raw material and do not remove cot, the embryo navel will be introduced a large amount of fat and influence the local flavor of beer, foam and add dark shade: 3, long reaction time, and complex process, plant factor is low, beer cost height.The object of the present invention is to provide a kind of Fructus Hordei Germinatus that do not adopt is raw material, and the quick and continuous brew that can eradicate the carcinogenic substance in the beer does not contain the brewing method of carcinogenic beer.

The object of the present invention is achieved like this: it is to be raw material with cereal, as being raw material with corn, rice, Zhou rice, millet, the seed of jog's tears, sorghum rice, barley, wheat, highland barley or their mixture, additional enzyme is made syrup for beer, brewages through the device fermentative processing of continuously fermenting then to become not contain carcinogens beer.

The raw material that the present invention selects for use is a corn fine powder of removing the embryo navel, remove rice (rice) fine powder of cot, remove the bright rice fine powder of cot, remove the glutinous rice fine powder of cot, remove cot millet fine powder, remove the seed of jog's tears fine powder of cot, remove the sorghum rice fine powder of cot, remove the barley fine powder of cot, remove the wheat fine powder of cot, remove a kind of in the highland barley fine powder of cot or their mixture.The used enzyme of the present invention is two groups of mixed enzyme, and it comprises microbial amylase, microorganism isoamylase, microbial glucoamylase, microbial protease, maltogenic amylase of plant and plant protease.The addition of enzyme is that per 100 parts of raw materials add first group of mixed enzyme 0,05-4 weight part, second group of mixed enzyme 0,03-3 weight part.

Comprise microbial amylase in first group of mixed enzyme, microbial protease and plant protease, comprise microbiology class amylase in second group of mixed enzyme, microbial glucoamylase or maltogenic amylase of plant, plant protease or microbial protease, wherein said enzyme comprises: microbial amylase, as BF7658 amylase (commercial goods each claim) etc., microbial protease, as 1,398 proteolytic enzyme, 166 proteolytic enzyme, 3942 proteolytic enzyme (each claims to be the commercial goods) etc., microbial glucoamylase, as β-saccharifying enzyme (commercial goods each claim) etc., maltogenic amylase of plant, as β-saccharifying enzyme (commercial goods each claim) etc., plant protease, as papain, Traumanase (each claims the commercial goods) etc.The microorganism isoamylase is as 10016 bacterial strain enzymes etc.Above-mentioned said two groups of mixed enzyme are respectively: in first group of mixed enzyme, microbial amylase accounts for 40-80%(weight), plant protease or microbial protease or their mixture account for 60-20%(weight).In second group of mixed enzyme, the microorganism isoamylase, microbial glucoamylase or maltogenic amylase of plant or it mixture account for 20-60%(weight), microbial protease or plant protease or their mixture account for 40-80%(weight).

Implementation step of the present invention is: drop into neutral brewing water 720-840 kilogram in brew kettle, under agitation add chemical pure calcium chloride 450-540 gram, add the corn fine powder 175-185 kilogram of removing the embryo navel, add first group of mixed enzyme 800-2000 gram under agitation makes enzyme thoroughly dissolve thereupon, corn fine powder is soaked into fully, transfer pH value 6,1-7,8, be incubated 120～140 minutes down at 40～60 ℃, and then accent pH value 6.1～7.3, solution is heated to 75-95 ℃ of insulation 20-40 minute, inspect with iodine liquid around here, when treating that solution is yellowish brown, stop to heat and cooling the temperature to below 60 ℃, add second group of mixed enzyme 1200-1500 gram again, be incubated 120～140 minutes down at 40～60 ℃, heat up then and boiled 3～15 minutes, reduce to 80～90 ℃ and remove corn groove slag with flame filter press, again syrup is squeezed in the boiling pot, add 1～1.2 kilogram in fresh hops, transfer pH value 5.2～5.4, boiled 40-60 minute, divide and remove the hops slag, the specialized syrup of beer of making is squeezed in high-order sealing or the sealing pressurized precipitation groove precipitation cooling to be used in order to fermentation procedure, continuous ferment process of the present invention, ferment wine (or claiming Primary Fermentation wine) entered back ferment narrow-necked earthen jar afterwards and made beer by traditional method before both can having continuously fermented by fermentation container, specialized syrup of beer is continuously once brewageed by the device that continuously ferments become not contain the carcinogens beer beer of (not needing secondary fermentation), through the thin plate water cooler reduce to-1-1.5 ℃ under, flow into by the road in the storage wine narrow-necked earthen jar, cut down door in this carbonic acid gas vent pipe of opening simultaneously on the device that continuously ferments, make the interior carbon dioxide gas of fermentation container through after the first water Washers and the purification of the second alcohol Washers, along with beer enters in the storage wine narrow-necked earthen jar, said Washers is the narrow-necked earthen jar body that water or alcohol are housed, this moment, a part of carbonic acid gas was absorbed by beer, part carbonic acid gas is full of storage wine narrow-necked earthen jar and discharges the interior air of narrow-necked earthen jar, surplus carbon dioxide reclaims or emptying, and can filter wine, can during through check analysis quality standard up to specification at 1-2 ℃ of following constant temperature 5-7 days.

The structured material of the device that continuously ferments of the present invention adopts carbon sheet to make, the liner acid-proof ceramics, glass, woodboard is fast, nontoxic acidproof paint or stainless steel materials are made or the like, the device that continuously ferments is a vertical tubular body arranged inside, 13000 liters of contents, (can increase and decrease capacity according to different output) two ends up and down respectively seals with the flange end socket, the wall outside is with the logical frozen water insulation of chuck, sieve plate is housed up and down in the fermentation container, adorn tensimeter on the fermentation container upper cover, emptying is cut down, carbonic acid gas vent pipe and temperature tube, the beer spillway that about 10 centimeters places establish under end socket, be connected the thin plate water cooler with pipeline, carbonic acid gas vent pipe (forward to insert) and storage wine narrow-necked earthen jar, the beer that continuous overflow comes out, reduce to-1-1.5 ℃ through the thin plate water cooler, the carbon dioxide gas of discharging, together flow in the storage wine narrow-necked earthen jar through Washers, beer absorption that carbonic acid gas is cooled around here, part carbonic acid gas then is full of the space of storage wine narrow-necked earthen jar and discharges the interior air of narrow-necked earthen jar, reach and purify the narrow-necked earthen jar body, prevent that beer from polluting, the lower cover of fermentation container is provided with sewage draining exit, temperature tube, sterile air adds pipe and syrup charging opening, on the charging opening in the pipeline connection traffic, the thin plate water cooler, high-order settling baths etc. are all to cut down gate control, newly formed fixed yeast sieve plate polylith is put into (more than two in the continuously ferment middle part of device, what determine with output and specific reactor the sieve plate number), or the suspension yeast body sieve plate polylith of putting into new processing is (more than two, what determine with output and specific reactor the sieve plate number), use the 5-40%(weight of yeast amount) as the syrup amount, the production method of fixed yeast sieve plate is identical with well-established law, it is through being processed into porous sieve plate with a kind of nonpoisonous and tasteless wooden boards, after operations such as boiling sterilization drying are handled, immerse in the zero yeast thin liquid again and adsorb yeast cell, and suspension yeast body sieve plate is with the 2-4 yeast slurry in generation, under aseptic technique, be packed into the thin cave on the wooden sieve plate of nonpoisonous and tasteless porous, micropore, channel and circumference duct, form integral body with equidistant from distance then and put into the device that continuously ferments, add syrup and continuously ferment, fermentation container of the present invention is that two (or group) parallel connections are used alternatingly to guarantee serialization in production system.Fermentation process of the present invention, both can disposablely continuously ferment and make beer, the ferment narrow-necked earthen jar continued fermentation after ferment wine (generally being called Primary Fermentation) flowed into before (no longer through ferment fermentation later) can continuously ferment again, its disposable continuous ferment process is, before beginning to continuously ferment, should be earlier with the device that continuously ferments (hereinafter to be referred as fermentation container) washing and sterilizing, putting into fixed yeast sieve plate or suspension yeast body sieve plate (the yeast consumption is syrupy 5-40% weight) then opens high-order settling bath pipeline thereupon and cuts down door, through water cooler syrup being reduced to about 9 ℃ of overflow port places that flow into fermentation containers closes and cuts down, seal immediately with anti-pollution, all water coolers on inspection, under meter, tensimeter, thermo detector, emptying is cut down, after carbonic acid gas vent pipe or the like road pipe cuts down a nothing leakage, opening emptying cuts down in fermentation container to be interrupted and sends into sterile air and impel yeast to quicken propagation, maintain the temperature at 9 ± 1 ℃, through about 275-80 hour, when treating that beer quality in the fermentation container reaches required standard, can open high-order precipitation barrel and cut down door, the syrup that flows down through water cooler reduce to about 10 ℃ with the about 2.8 liters flow velocity of per minute under meter enters fermentation container, the upflow tube of opening fermentation container simultaneously cuts down door, make beer in the fermentation container enter water cooler to reduce to-1-1.5 ℃ with the flow velocity that is equal to, open the carbonic acid gas vent pipe simultaneously in this and cut down door, make in the fermentation container carbon dioxide gas through the first water Washers and the second alcohol Washers purify the back along with reduce to-beer of 1-1.5 ℃ flows in the storage wine narrow-necked earthen jar, a part of around here carbonic acid gas is absorbed by beer, part carbonic acid gas enters storage wine narrow-necked earthen jar and gets rid of air in the narrow-necked earthen jar, purified the narrow-necked earthen jar body and prevented that beer from polluting, surplus carbon dioxide recovery or emptying were also left standstill under 1-2 ℃ constant temperature 5-7 days, can filter wine, can during quality standard up to specification by analysis.The method of ferment wine is before continuously fermenting, when the syrupy concentration in the fermentation container is reduced to 4.5-5.5BX, can open high-order precipitation barrel and cut down door, the syrup that flows down is reduced to 9 ℃ through water cooler, with the flow velocity of 3.3 liters of per minutes in flowing to fermentation container, the upflow tube of opening fermentation container simultaneously cuts down door, with the flow that is equal to through water cooler reduce to 2-3 ℃ enter after other working method of ferment narrow-necked earthen jar carbonic acid gas emptying identical with traditional fermentation beer method.

For example method of the present invention is elaborated below

Example 1, in saccharification tin, drop into 720 kilograms of neutral brewing waters, under agitation add chemical pure calcium chloride 500 grams, add 185 kilograms of corn fine powders removing the embryo navel, add first group of mixed enzyme, 2000 grams thereupon, enzyme is thoroughly dissolved, corn fine powder is soaked into fully, transfer pH value 7.0, be incubated 140 minutes down at 43 ± 1 ℃, transfer pH value 6.4 afterwards, solution is heated to 89 ℃ then, be incubated 38 minutes, inspect with iodine liquid around here, treat to be cooled to when solution is yellowish brown 49 ℃, drop into second group of mixed enzyme, 1500 grams again, be incubated 140 minutes down at 51 ℃, heat up again and boiled 3 minutes, reduce to 80～90 ℃ of elimination corn groove slags, again syrup is squeezed in the boiling pot, add 1.2 kilograms in fresh hops, transfer pH value 5.3, boil 40 minutes branches and remove the hops slag, the specialized syrup of beer of making is pumped into high-order sealing or seals precipitation cooling in the settling bath that pressurizes, here illustrate at first that one-time continuous fermentation brews the fermentation process of beer into, before beginning to continuously ferment, should wash away with the device that continuously ferments (hereinafter to be referred as fermentation container) washing and sterilizing or with high pressure steam earlier, then fixed yeast sieve plate or the suspension yeast sieve plate of making dropped in the fermentation container, the yeast consumption is generally the 5-40%(weight of syrup consumption) open high-order settling bath pipeline thereupon and cut down door, make the interior syrup of groove reduce to 9 ℃ of overflow port places that flow to fermentation container, seal immediately, check high-order settling bath simultaneously with anti-pollution through the thin plate water cooler, the thin plate water cooler, the pipeline between under meter and the fermentation container, cut down door.Check and connect fermentation container, the thin plate water cooler, pipeline between carbonic acid gas escape route and storage wine narrow-necked earthen jar or the back ferment narrow-necked earthen jar, whether cut down door reveals, check tensimeter, insert thermometer, close stopple coupon, opening emptying cuts down, when comprehensive review thinks errorless, again the sterile air pipe is cut down and open, interruption is sent into sterile air and is impelled Yeast proliferation in fermentation container, then make the syrup fermentation, approximately through 75-80 hour, when treating that beer quality in the fermentation container reaches specified standards, can open high-order settling bath pipeline and cut down door, the syrup that flows down is reduced to about 10 ℃ through the thin plate water cooler, with the about 2.8 liters flow velocity of per minute under meter enters fermentation container, the upflow tube of opening fermentation container simultaneously cuts down door, make the beer in the fermentation container enter the thin plate water cooler and reduce to-1-1.5 ℃ with flow velocity with amount, open the carbonic acid gas vent pipe simultaneously in this and cut down door, after second Washers that makes first Washers of carbonic acid gas through cold water is housed and alcohol is housed purifies along with reduce to-the cold beer line of 1-1.5 ℃ flows into and stores in the wine narrow-necked earthen jar, carbon dioxide gas around here, a part is absorbed by beer, a part be full of storage wine narrow-necked earthen jar get rid of purifying air in the narrow-necked earthen jar narrow-necked earthen jar body prevent that beer from polluting, surplus carbon dioxide is through reclaiming or emptying, when reaching required standard, the check analysis beer quality can filter wine in 5-7 days leaving standstill under 1-2 ℃, can, next is the method for ferment wine before continuously fermenting, working method is the same, when just the syrup concentration in being full of fermentation container is reduced to 4.5-5.5BX, can open high-order settling bath pipeline and cut down door, the syrup that flows down is reduced to 9 ℃ through the thin plate water cooler, flow into fermentation container for 3.3 liters with per minute, the upflow tube of opening fermentation container simultaneously cuts down door, reduce to 2-3 ℃ with the flow that is equal to through the thin plate water cooler, enter back ferment narrow-necked earthen jar carbonic acid gas emptying, other secondary fermentation operational administratives are all identical with traditional fermentation beer method.

Example 2, composition of raw materials: remove 100 kilograms of the wheat fine powders of cot, remove 85 kilograms in the rice of cot, add first group of mixed enzyme, 1800 grams, second group of mixed enzyme 1200 gram, other method for saccharifying, the fermented soy method is all same with example 1.

Example 3, composition of raw materials: remove 150 kilograms of the barley fine powders of cot, 55 kilograms of sorghum rice fine powders add first group of mixed enzyme, 2000 grams, second group of mixed enzyme 1500 gram, and other method for saccharifying, the fermented soy method is all same with example 1.

Example 4, composition of raw materials: remove 150 kilograms of the wheat fine powders of cot, remove 40 kilograms of the millet fine powders of cot, add first group of mixed enzyme, 1800 grams, second group of mixed enzyme 1200 gram, other method for saccharifying, the fermented soy method is all same with example 1.

Example 5, composition of raw materials: remove 160 kilograms of the wheat fine powders of cot, remove 50 kilograms of the barley fine powders of cot, add first group of mixed enzyme, 1700 grams, second group of mixed enzyme 1100 gram, other method for saccharifying, the fermented soy method is all same with example 1.

The invention has the advantages that:

1, raw material is domestic does not need import, and the wide material sources price is inexpensive, and generations are inexhaustible. To remove the corn fine powder of embryo navel, remove a kind of in the rice of cot, bright rice, glutinous rice, millet, the seed of jog's tears, sorghum rice, barley, wheat, highland barley, the oat or their mixture is raw material, external enzyme is made syrup for beer, beer through fermented soy, carcinogen N-nitrosodimethylamine and AFB1 have been eradicated fully, favourable health. The predecessor carrotene that particularly contains anticancer specific factor glutathione and cancer-resisting substance vitamin A in the corn therefore take the beer of corn as the raw material brew, does not only contain carcinogenic substance but also can also play protective effect on cancer risk.

2, the novel technical method of the beer brewing that continuously ferments fast of the present invention has changed existing discontinuous Da Chi fermentation process, reduces to take up an area, and energy savings improves output, reduces cost. To produce one ton of beer as example: adopt well-established law brewage process primary fermentation to take 7-9 days, adopt brewing method of the present invention then primary fermentation only need 36-48 hour.

3, the present invention selects domestic commercial microbiology class amylase, microbial amylase, microbial glucoamylase, microbial protease, maltogenic amylase of plant and plant rennet not only are convenient to not contain the large scale industry of carcinogenic substance beer and are produced, and have more promoted the Enzymes Industry tremendous development.

4, adopt brewing method of the present invention, the beer of brewageing is not except containing the carcinogenic substance, and its physical and chemical index, sense index all meet national GB4729-85. Health refers to meet fully national GB2758-81 standard.

Claims (6)

1; a kind of brewing method that does not contain carcinogenic beer of brewageing continuously; it comprises produces syrup and fermenting process; it is characterized in that: be raw material with cereal; add first group of mixed enzyme 0.05-4 weight part in per 100 weight part raw materials; the chloride enzyme protecting agent of second group of mixed enzyme 0.03-3 weight part and 0.12-0.3 weight part; wherein two groups of mixed enzyme are: first group of mixed enzyme comprises microbial amylase; plant protease or microbial protease; second group of mixed enzyme comprises; the microorganism isoamylase; microbial glucoamylase or maltogenic amylase of plant; plant protease or microbial protease; its brewing method is: drop into neutral brewing water and under agitation add chemical pure calcium chloride in brew kettle; add cereal materials and first group of mixed enzyme; enzyme is thoroughly dissolved; cereal materials is soaked into fully; transfer pH value 6.1-7.8; be incubated 120～140 minutes down at 40～60 ℃; again pH value is transferred to 6.1～7.8 afterwards; then solution is heated to 75-95 ℃ of insulation 20-40 minute; inspect with iodine liquid around here and cool the temperature to below 60 ℃ when treating that solution is yellowish brown; add second group of mixed enzyme again; be incubated 120-140 minute down at 40-60 ℃; intensification was boiled 3-15 minute; reduce to 80-90 ℃ and cross the poor slag of elimination; again this syrup is squeezed in the boiling pot; add fresh hops; transfer pH value 5; 2-5; 4; boiled 40-60 minute; divide and remove the hops slag; the specialized syrup of beer of making is pumped in the high-order settling bath precipitation cooling, but this syrup through continuously fermenting device not only the one-time continuous fermentation make beer but also can continuously ferment before ferment wine flow into then in the ferment narrow-necked earthen jar of back and proceed fermentation until reaching beer definite quality standard.

2; brewing method according to claim 1; the wherein said device that continuously ferments is vertical cast; each seals the two ends up and down of this fermentation container with the flange end socket; wall outside weldings chuck feeds the frozen water insulation; each attached sieve plate up and down in the device; on the upper cover of fermentation container tensimeter is housed; emptying is cut down; carbonic acid gas vent pipe and temperature tube; about 10cm place is provided with the beer overflow port under end socket; connect the thin plate water cooler with pipeline; carbonic acid gas vent pipe and storage wine narrow-necked earthen jar; the beer that overflows is reduced to-1～-1.5 ℃ of carbon dioxide gas with discharge through water cooler and is flowed in the lump in the storage wine narrow-necked earthen jar; a part of around here carbonic acid gas is absorbed by beer; a part is full of storage wine narrow-necked earthen jar, and to discharge in the narrow-necked earthen jar air conservation wine body not contaminated; surplus carbon dioxide reclaims or emptying; the lower cover of fermentation container is provided with blowdown and cuts down; temperature tube; sterile air adds pipe and syrup charging opening; charging opening is in the pipeline connection traffic; water cooler; high-order settling bath is all to cut down gate control; fixed yeast sieve plate or suspension yeast body sieve plate are put in the centre of fermentation container; the fixed yeast sieve plate is to make with nonpoisonous and tasteless porous wooden boards; behind sterilizing-drying, immerse zero for adsorbing yeast cell in the zymic thin liquid; suspension yeast body sieve plate then is for yeast slurry with 2-4; under aseptic technique, be filled in the duct on the nonpoisonous and tasteless wooden porous sieve plate; the whole adding in the fermentation container on the crisscross channel, formed with equidistant from distance in thin cave.

3, according to the brewing method of claim 1, in wherein said first group of mixed enzyme, microbial amylase accounts for 40-80%(weight) plant protease or microbial protease or their mixture account for 60-20%(weight).

4, according to the brewing method of claim 1, in wherein said second group of mixed enzyme, the microorganism isoamylase, microbial glucoamylase or maltogenic amylase of plant or their mixture account for 20-60%(weight), plant protease or microbial protease or their mixture account for 80-40%(weight).

5, brewing method according to claim 1, wherein said enzyme comprises: microbial amylase, as 7658 amylase (commercial goods each claim) etc., the microorganism isoamylase, as the different enzyme of 10016 bacterium (commercial goods each claim) etc., microbial glucoamylase, as β-saccharifying enzyme (commercial goods each claim) etc., microbial protease, as 1398 proteolytic enzyme, 3942 proteolytic enzyme, 166 proteolytic enzyme (each claims to be the commercial goods) etc., plant protease, as papoid, bromelines (be commercial goods each claim) etc., maltogenic amylase of plant is as B-saccharifying enzyme (each claims the commercial goods) etc.

6, according to the brewing method of claim 1, wherein said material grain is: remove the corn fine powder of embryo navel, remove the rice fine powder of cot, remove the Zhou rice fine powder of cot, remove the glutinous rice fine powder of cot, remove the millet fine powder of cot, remove the sorghum rice fine powder of cot, remove the seed of jog's tears fine powder of cot, remove the wheat fine powder of cot, remove the barley fine powder of cot, remove the highland barley fine powder of cot, remove a kind of in the oat fine powder of cot or their mixture.