CN106546537A - A kind of detection means and its detection method of protein characteristic identification - Google Patents
A kind of detection means and its detection method of protein characteristic identification Download PDFInfo
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- CN106546537A CN106546537A CN201610935699.XA CN201610935699A CN106546537A CN 106546537 A CN106546537 A CN 106546537A CN 201610935699 A CN201610935699 A CN 201610935699A CN 106546537 A CN106546537 A CN 106546537A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/1717—Systems in which incident light is modified in accordance with the properties of the material investigated with a modulation of one or more physical properties of the sample during the optical investigation, e.g. electro-reflectance
- G01N2021/1725—Modulation of properties by light, e.g. photoreflectance
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/1717—Systems in which incident light is modified in accordance with the properties of the material investigated with a modulation of one or more physical properties of the sample during the optical investigation, e.g. electro-reflectance
- G01N2021/1727—Magnetomodulation
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Abstract
The present invention provides a kind of detection means of protein characteristic identification, including：Light source, for providing the polarized light of different polarization state, different wave length；Substrate, is arranged on the side of the light source, and the substrate is mounted with liquid protein；Field mechanisms, for the liquid protein orientation adsorbed to the substrate surface and the control for arranging, the field mechanisms are arranged on the relative both sides of the substrate；And prototype test platform；Wherein, the substrate that will be loaded with liquid protein is fixed on the prototype test platform as print and is tested, and as axle, the plane of the print can pivot for the direction of propagation of the light sent with the light source.The present invention also provides a kind of detection method of protein characteristic identification.The technical scheme that the present invention is provided has extraordinary repeatability and accuracy.
Description
Technical field
The present invention relates to protein detection field, more particularly to a kind of detection means and its detection of protein characteristic identification
Method.
Background technology
At present, the main method of protein characteristic identification has X diffractive technologies, nuclear magnetic resonance technique, CD technologies etc..These sides
Method is used for the differential expression for studying protein model under the conditions of different measuring, there is provided the protein structural information of different aspects, enters
Row protein functional assays.
In practice, said method respectively has pluses and minuses, for example：X-ray diffraction method is virus protein feature identification
Important experimental technique, many existing virus protein AUTHORITATIVE DATAs both are from X-ray diffraction analysis, however, suitable dimension and height
The highly difficult property of quality protein crystal production, is a bottleneck in the method application, additionally, analytical cycle length, being not easy
The relation of 26S Proteasome Structure and Function under protein physiological statuss is illustrated, also makes the application of the method there is limitation；Nuclear magnetic resonance technique
It is currently the only especially to exist in the important experimental technique of atom definition level determination Proteins In Aqueous Solutions molecule stereo structure
In terms of virus protein feature identification, nuclear magnetic resonance, NMR has extremely had successfully formed a kind of complementary relationship with X diffraction methods, but nuclear-magnetism is altogether
Vibration Meter device is complicated, expensive, data analysiss amount big, causes the technical method to be difficult popularization and application；CD technologies can be used for biology
The steric configuration of macromolecule polyalcohol, the static research of conformation, it is also possible to which the dynamic to show these polymer space conformations becomes
Change process, but due to the limitation of Method And Principle, CD technologies cannot also directly provide the concrete of protein molecule and surface binding site
Information.
So far, the various means of testing related to protein molecule orientation are all due to protein immobilization techniques, orientation
Control and characterizing method aspects there is such or such defect and fail obtain it is intended that effect.On the other hand, people
Control the ability of microenvironment system and can not meet the requirements of great majority experiment, many has influence on the technical parameters of Detection results
Can only be obtained by indirect method, this also brings many difficulties to the data process＆analysis in experiment later stage.Although, using superpower
Magnetic field (such as H ＞ 10T) can reach the purpose of Proteins In Aqueous Solutions tropism control, and then realize protein orientation and aligned orderly
Change, partly obtain some effects that the micro- display technology of protein has, but, build the ultrastrong magnetic field of such rank
And maintaining its day-to-day operation pay high expense, this is that a research institution can not irrespective realistic problem.
The content of the invention
In view of this, it is an object of the invention to provide a kind of detection means of protein characteristic identification and its detection side
Method, it is intended to which the repeatability and accuracy for solving feature identification in prior art is all relatively low, and the higher problem of experimental cost.
The present invention proposes a kind of detection means of protein characteristic identification, mainly includes：
Light source, for providing the polarized light of different polarization state, different wave length；
Substrate, is arranged on the side of the light source, and the substrate is mounted with liquid protein；
Field mechanisms, for liquid protein orientation and the control for arranging, the magnetic field adsorbed to the substrate surface
Mechanism is arranged on the relative both sides of the substrate；And
Prototype test platform；
Wherein, the substrate that will be loaded with liquid protein is fixed on the prototype test platform as print and is surveyed
Examination, as axle, the plane of the print can pivot for the direction of propagation of the light sent with the light source.
On the other hand, the present invention also provides a kind of detection method of protein characteristic identification, mainly includes：
Prepare substrate；
Prepare print；
Build the detection means of the protein characteristic identification as described in above-mentioned any one；
The detection means recognized using the protein characteristic in three dimensions by adjust the intensity in magnetic field, direction with
And magnetic field and the substrate surface angle, carry out protein characteristic identification.
The technical scheme that the present invention is provided, by Interfacial Adsorption and the synergism in magnetic field, forms in substrate surface and has
The protein function interface of microarray property, then, incident polarized light is interacted with protein microarray, carries interface egg
The information of white matter 26S Proteasome Structure and Function, supplies protein characteristic identification used after data process＆analysis, and this device is eliminated
One ultrastrong magnetic field for needing expensive expense, greatly reduces construction and the maintenance cost of similar test device, with very big
Commercial value.Simultaneously with line polarized light to analyze light beam, using magnetic field and the synergism of Interfacial Adsorption, generate in substrate surface
One protein orientation and arrange the microarray in ordering feature, this method of testing not only have higher measurement sensitivity and
Resolution, and also confirmed that by the test result to a large amount of different proteins, the method is applied to into protein characteristic identification,
Show extraordinary repeatability and accuracy.
Description of the drawings
Fig. 1 is the internal structure schematic diagram of the detection means of protein characteristic identification in an embodiment of the present invention；
Fig. 2 be an embodiment of the present invention in uniform magnetic field (H=180mT), print reflected light signal intensity
IBSA-O-ISFor the function of print azimuth rotation angle α, wherein BSA percentage concentrations are 0.5%；
Fig. 3 is in an embodiment of the present invention, in uniform magnetic field H span is 60～360mT, print reflected light is believed
Number intensity IBSA-O-ISFor the function of magnetic field intensity H, wherein BSA percentage concentrations are 0.5%；
When Fig. 4 is to exist and there is no 180mT uniform magnetic fields in an embodiment of the present invention, reflected light signal intensity
IBSA-O-ISChange with print azimuth rotation angle α, wherein BSA percentage concentrations are 0.5%；
Fig. 5 be an embodiment of the present invention in uniform magnetic field, variable concentrations BSA sample characteristics parameter, Δ IBSA-O-ISWith
Magnetic field intensity H changes, wherein ▲ BSA percentage concentrations 0.5% are represented, ■ represents BSA percentage concentrations 2.0%；
Fig. 6 is there is (■) and when there is no (▲) 180mT uniform magnetic fields in an embodiment of the present invention, through quartz
The light signal strength I of BSA solution in pondBSAWith the change that quartz cell occurs around coordinate axess (Y) rotation angle α difference；
Fig. 7 is to be respectively 0 ° in uniform magnetic field (H=180mT) direction and print surface angle in an embodiment of the present invention
(■), corresponding I when 11.5 ° (●) and 23 ° (▼)BSA-O-IS(α), wherein BSA percentage concentrations 0.5%；
During Fig. 8 is 655nm light sources for λ in an embodiment of the present invention, INS-BSAAnd I (H)DS-O-BSA(H) comparison diagram；
During Fig. 9 is 405nm light sources for λ in an embodiment of the present invention, INS-BSAAnd I (H)DS-O-BSA(H) comparison diagram.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, it is below in conjunction with drawings and Examples, right
The present invention is further elaborated.It should be appreciated that specific embodiment described herein is only to explain the present invention, and
It is not used in the restriction present invention.
The technical scheme that the present invention is provided, with line polarized light to analyze light beam, is made using collaboration of the magnetic field with Interfacial Adsorption
With in one protein orientation of substrate surface generation and microarray of the arrangement in ordering feature, based on this experimental principle
Method of testing not only has higher measurement sensitivity and resolution, the test result of a large amount of different proteins is also confirmed that, at this
Method is applied to protein characteristic identification, it is shown that extraordinary repeatability and accuracy.
A kind of detection means of protein characteristic identification provided by the present invention will be described in detail below.
Fig. 1 is referred to, is that the internal structure of the detection means of protein characteristic identification in an embodiment of the present invention is illustrated
Figure.
In the present embodiment, the detection means of protein characteristic identification includes：
Light source, for providing the polarized light of different polarization state, different wave length；
Substrate, is arranged on the side of the light source, and the substrate is mounted with liquid protein；
Field mechanisms, for liquid protein orientation and the control for arranging, the magnetic field adsorbed to the substrate surface
Mechanism is arranged on the relative both sides of the substrate；And
Prototype test platform；
Wherein, the substrate that will be loaded with liquid protein is fixed on the prototype test platform as print and is surveyed
Examination, as axle, the plane of the print can pivot for the direction of propagation of the light sent with the light source.
In the present embodiment, the light source includes the LASER Light Source being linked in sequence successively, circularly polarized light generator, is polarized
Prism and diaphragm.The light source can also be the laser aid that other can provide different polarization states, different wave length.
In the present embodiment, the substrate includes the naked substrate made by homogenous material or by multilayer material making
Composite substrate.
In the present embodiment, the field mechanisms are specifically for the intensity by adjusting magnetic field in three dimensions, side
To and magnetic field and the substrate surface angle, realize to the substrate surface absorption liquid protein orientation with arrangement
Control.
In the present embodiment, the prototype test platform includes：First reflecting mirror, the second reflecting mirror, print fix dress
Put and vernier element；
Wherein, first reflecting mirror and second reflecting mirror are respectively positioned on the same side of the diaphragm, and swash from described
The light that radiant sends is mapped to described first after sequentially passing through the circularly polarized light generator, the polarizing prism, the diaphragm
Incident illumination is reflexed to the substrate for being mounted with liquid protein, the table of substrate described in Jing by reflecting mirror, first reflecting mirror
Second reflecting mirror is injected after the reflection of face, and emergent light is formed after the reflection of second reflecting mirror and projected.
In the present embodiment, the detection means also includes：Analyzing prism, photo-detector and control and data processing
System；
Wherein, it is injected into the direction of propagation of the incident illumination of first reflecting mirror and projects from second reflecting mirror
The emergent light the direction of propagation it is identical and be parallel to each other, the emergent light injects the analyzing prism and through the analyzing
Prism enters the photo-detector, and the photo-detector is detected to the emergent light that the analyzing prism is received, and
The result data of detection is sent into into the control process is analyzed with data handling system.
As shown in figure 1, being that measuring system coordinate y-axis is incident light propagation direction in the thick dashed line square frame of the upper right corner, xOz is to treat
Test sample piece place plane, system coordinates lower left be the present invention protein characteristic identification detection means, example platform RSP positions
In a uniform magnetic field, during measurement, print is rotated by the center of circle of O in xOz planes around y-axis.During measurement, room temperature is maintained at 20
～25 DEG C.
In FIG, PD represents photo-detector (such as CR114), and RSP (Rotatable sample platform) is expressed as
The example platform that can be rotated around Y-axis, Laser are LASER Light Source, and QMP is circularly polarized light generator, and H is magnetic field, and P1, P2 are respectively
Be polarized, analyzing prism, G is diaphragm, and Computer is control and data handling system.In measuring system coordinate, α is print orientation
The angle that (in figure shown in fine dotted line) is rotated.
On the other hand, the present invention also provides a kind of detection method of protein characteristic identification, mainly includes：
Prepare substrate；
Prepare print；
Build the detection means of the protein characteristic identification as described in above-mentioned any one；
The detection means recognized using the protein characteristic in three dimensions by adjust the intensity in magnetic field, direction with
And magnetic field and the substrate surface angle, carry out protein characteristic identification.
In the present embodiment, the substrate includes substrate of glass, iron based nano crystal substrate and silvered substrates, wherein institute
The step of stating preparation substrate includes：
The preparation of substrate of glass：Thick 1mm glass board materials are taken, and area are processed into for 10 × 18mm2Rectangle glass piece make
For substrate of glass (naked substrate)；
The preparation of iron based nano crystal substrate：Thick 20 μm iron based nano crystal band is pasted on the glass substrate, after solidification
Polish, be polished to minute surface, to form iron based nano crystal substrate (composite substrate)；
The preparation of silvered substrates：Vacuum plating silver, forms SERS substrate (composite substrate), thickness of coating on the glass substrate
For 100nm.
In the present embodiment, it is described to include the step of prepare print：
3 μ l of sample to be tested (i.e. protein solution) are taken with sample presentation device, drop covers thick in the substrate surface, on drop
130 μm of slides, stand 1～2min, load the prototype test platform and be fixed survey after liquid layer under coverslip is evenly distributed
Examination.Wherein, experiment reagent：Bovine serum albumin (BSA), human serum albumin (HAS), human serum hemoglobin (HGB), immunity
Lysozyme (IgG), Quality Control human serum standard specimen (507UEH, 844UN) and C reactive protein (CRP) respectively purchased from sigma companies,
Randox companies and Chinese Shanghai Flos Lonicerae bio tech ltd, other auxiliary reagents are public purchased from Chinese Shanghai chemical reagent
Department；Clinical human serum sample is provided by China Shenzhen city women and children hospital laboratory；The distilled water and deionized water of about 1 μ S electrical conductivity
It is used for all experiments.
In the present embodiment, the detection method also includes：
The print in light path is positioned before measurement, and set the default sampling time, with protein sample during ensureing measurement
All the time it is in static liquid.
In the present embodiment, characterize for data acquisition and protein characteristic, it is strong with protein print reflected light signal
The test that the test curve I (α) and reflected light signal intensity I that degree I changes with print azimuth rotation angle α changes with magnetic field intensity H
Curve I (H) characterizes protein sample feature.If without especially indicating, wherein all experimental optical source wavelength λ are 532nm.
The measurement result obtained by experiment shows：(1) when protein print is below 0.5T uniform magnetic fields in an intensity
During middle rotation, show periodically from the signal light intensity I of print surface reflection with the change (angle step pitch Δ α=2 °) of rotation angle α,
If initial azimuth α is 90 ° when print rotates, if intercepting the map sheet between 90 °～138 ° of test curve, the scope build-in test is found
Curve has shown a complete cycle (see Fig. 2), therefore the sectional drawing area is set to measure sampling interval, and definition signal light intensity I
Fluctuation amplitude, ao I=Imax-IminFor a characteristic parameter of test curve I (α).(2) in above-mentioned sampling interval, do not change
Protein print azimuth, is altered in steps uniform magnetic field intensity (step pitch Δ H=20mT) and samples successively, finds from print surface
The change of reflected light signal intensity I is presented a kind of monotone increasing trend, and its form is similar to straight line (see Fig. 3).It is linear
After fitting, another characteristic parameter that linear equation slope k is test curve I (H) is taken.In this measuring method, Δ I and k are used
In sign Interfacial Adsorption protein characteristic.
In the present embodiment, adsorb and magnetic field effect for solid liquid interface protein, as an example of magnetic field effect
Son, Fig. 4 are two test curves of protein sample when existing and there is no a 180mT uniform magnetic field, as seen from the figure,
In 180mT uniform magnetic fields, protein print shows regular anisotropy to incident ray polarized light, and test curve is presented week
Phase property, this illustrates that print median surface adsorbed proteins have and is orientated the consistent, feature of aligned orderly；When magnetic field does not have (H=0)
When, test curve tendency is irregular state, illustrates protein print median surface adsorbed proteins orientation in random distribution, arrangement
It is unordered.Further experiment also shows：1), test curve morphological characteristic is associated (refering to Fig. 5) with protein concentration in sample；
2), the result that magnetic field effect is produced not only is associated with magnetic field intensity, also the directional correlation (referring to Fig. 5, Fig. 7 and Biao 1) with magnetic field；
3), when this condition of Interfacial Adsorption is lacked, a low intensive uniform magnetic field is difficult to affect taking for Proteins In Aqueous Solutions molecule
To and sequence.As shown in fig. 6, no matter magnetic field whether there is, in quartz cell, protein solution is all shown as to incident ray polarized light
Isotropism, illustrates that Proteins In Aqueous Solutions molecule has the orientation of all directions all the time, and the presence in magnetic field does not change this
Situation.
I in 1 direction of table difference uniform magnetic field (H=180mT)BSA-O-IS(α) characteristic parameter contrast
1 experimental data of table shows that magnetic direction has a great impact to protein test curve morphological characteristic, changes even strong
During the direction in magnetic field, IBSA-O-IS(α) characteristic parameter Δ I there occurs significant change.
The impact that magnetic field is orientated to Interfacial Adsorption protein molecule, is to change the azimuthal direction of binding molecule by magnetic field
With molecule the inclination angle on surface realized.From Fig. 7 and Biao 1, magnetic direction plays highly important work to I (α) morphological characteristic
With, by it is determined that the azimuth direction of Interfacial Adsorption protein molecule be the principal element that affects interfacial protein matter orientation,
It is to build one of the essential condition at protein function interface.
In the present embodiment, take light sources with different wavelengths and make Experimental comparison.Fig. 8 and 9 show light source take respectively λ=
During 655nm and λ=405nm, native state BSA and change condition BSA prototype test curve IBSA(H).Wherein, the I of Fig. 8NS-BSA(H)=
1.902H+2948, IDS-O-BSA(H)=1.3612H+3139；The I of Fig. 9NS-BSA(H)=2.7772H+4179, IDS-O-BSA(H)=
1.1482H+5054。
Table 2INS-BSAAnd I (H)DS-BSA(H) contrast of characteristic parameter k
Table 3INS-BSAAnd I (H)DS-BSA(H) contrast of characteristic parameter k
Wherein, kNS-BSAAnd kDS-BSARespectively INS-BSAAnd I (H)DS-BSA(H) characteristic parameter, optical source wavelength λ=405mT；
k’NS-BSAAnd k 'DS-BSARespectively I 'NS-BSAAnd I ' (H)DS-BSA(H) characteristic parameter, optical source wavelength λ=655mT；p1=∣
kNS-BSA- kDS-BSA∣/kNS-BSAOr p1=∣ k 'NS-BSA- k 'DS-BSA∣/k’NS-BSA；p2=∣ k 'NS-BSA- kNS-BSA∣/kNS-BSAOr p2
=∣ k 'DS-BSA- kDS-BSA∣/k’DS-BSA。
2 experimental data of table shows, BSA from native state change to condition when, the slope k of I (H) can change, and because
Optical source wavelength λ is different and there are different amplitudes of variation：During λ=655nm, kNS-BSAAmplitude of variation is 28.43%, λ=405nm
When, kNS-BSAAmplitude of variation is 58.66%, and the latter is slightly larger than the former twice.3 experimental data of table shows：Work as incident ray polarized light
Wavelength X change when, native state and become condition IBSA(H) slope k all can change therewith, but they have not with the change of wavelength
Same response sensitivity (Sλ), optical source wavelength λ from 655nm be changed into 405nm when, INS-BSA(H) slope kNS-BSAChange amplitude is
46.01%, and IDS-BSASlope kDS-BSAChange amplitude only 15.65%, the former is almost three times of the latter.Above-mentioned experimental result
Illustrate, when protein optical activity is characterized with line polarized light, should take into full account that protein optical dispersion and circular dichroism absorb right
The contribution of light signal strength, selects the light source for being adapted to wavelength to be greatly improved sensitivity and the resolution of detection method.
The detection means and its detection method of a kind of protein characteristic identification that the present invention is provided, by Interfacial Adsorption and magnetic
Synergism, form the protein function interface with microarray property, then, incident polarized light and egg in substrate surface
White matter microarray interacts, and carries the information of interfacial protein matter 26S Proteasome Structure and Function, and egg is supplied after data process＆analysis
Used by white matter feature identification, and this device eliminates a ultrastrong magnetic field for needing expensive expense, greatly reduces same class testing
The construction of device and maintenance cost, with very big commercial value.Simultaneously with line polarized light to analyze light beam, using magnetic field and boundary
The synergism of face absorption, generates a protein orientation in substrate surface and arranges the microarray in ordering feature, this
Method of testing not only has higher measurement sensitivity and resolution, and is also demonstrate,proved by the test result to a large amount of different proteins
It is bright, the method is applied to into protein characteristic identification, it is shown that extraordinary repeatability and accuracy.
It should be noted that in above-described embodiment, what included unit was simply divided according to function logic,
But above-mentioned division is not limited to, as long as corresponding function can be realized；In addition, the specific name of each functional unit
Only to facilitate mutually distinguishing, protection scope of the present invention is not limited to.
In addition, one of ordinary skill in the art will appreciate that realizing all or part of step in the various embodiments described above method
Program be can be by instruct the hardware of correlation to complete, corresponding program can be stored in embodied on computer readable storage and be situated between
In matter, described storage medium, such as ROM/RAM, disk or CD etc..
Presently preferred embodiments of the present invention is the foregoing is only, not to limit the present invention, all essences in the present invention
Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.
Claims (10)
1. the detection means that a kind of protein characteristic is recognized, it is characterised in that the detection means includes：
Light source, for providing the polarized light of different polarization state, different wave length；
Substrate, is arranged on the side of the light source, and the substrate is mounted with liquid protein；
Field mechanisms, for the liquid protein orientation and the control for arranging, the field mechanisms that adsorb to the substrate surface
It is arranged on the relative both sides of the substrate；And
Prototype test platform；
Wherein, the substrate that will be loaded with liquid protein is fixed on the prototype test platform as print and is tested, with
The direction of propagation of the light that the light source sends is axle, and the plane of the print can pivot.
2. the detection means that protein characteristic as claimed in claim 1 is recognized, it is characterised in that the light source includes suitable successively
LASER Light Source, circularly polarized light generator, polarizing prism and diaphragm that sequence connects.
3. the detection means that protein characteristic as claimed in claim 1 is recognized, it is characterised in that the light source is can to provide not
The laser aid of same polarization state, different wave length, the substrate include the naked substrate made by homogenous material or by multilayer material
The composite substrate of making.
4. the detection means that protein characteristic as claimed in claim 1 is recognized, it is characterised in that the field mechanisms are specifically used
In the intensity by adjusting magnetic field in three dimensions, direction and magnetic field and the substrate surface angle, realize to described
The liquid protein orientation of substrate surface absorption and the control of arrangement.
5. the detection means that protein characteristic as claimed in claim 2 is recognized, it is characterised in that the prototype test platform bag
Include：First reflecting mirror, the second reflecting mirror, print fixing device and vernier element；
Wherein, first reflecting mirror and second reflecting mirror are respectively positioned on the same side of the diaphragm, and from the laser light
The light that source sends is mapped to first reflection after sequentially passing through the circularly polarized light generator, the polarizing prism, the diaphragm
Incident illumination is reflexed to the substrate for being mounted with liquid protein by mirror, first reflecting mirror, and described in Jing, the surface of substrate is anti-
Second reflecting mirror is injected after penetrating, and emergent light is formed after the reflection of second reflecting mirror and projected.
6. the detection means that protein characteristic as claimed in claim 5 is recognized, it is characterised in that the detection means is also wrapped
Include：Analyzing prism, photo-detector and control and data handling system；
Wherein, the direction of propagation of the incident illumination of first reflecting mirror and the institute projected from second reflecting mirror are injected into
The direction of propagation for stating emergent light is identical and be parallel to each other, and the emergent light injects the analyzing prism and through the analyzing prism
Into the photo-detector, the photo-detector is detected to the emergent light that the analyzing prism is received, and will inspection
The result data of survey is sent into the control and is analyzed process with data handling system.
7. the detection method that a kind of protein characteristic is recognized, it is characterised in that the detection method includes：
Prepare substrate；
Prepare print；
Build the detection means of the protein characteristic identification as any one of above-mentioned claim 1-6；
Pass through in three dimensions to adjust intensity, direction and the magnetic in magnetic field using the detection means that the protein characteristic is recognized
Field and the substrate surface angle, carry out protein characteristic identification.
8. the detection method that protein characteristic as claimed in claim 7 is recognized, it is characterised in that the species of the substrate includes
Substrate of glass, iron based nano crystal substrate and silvered substrates, wherein including the step of the preparation substrate：
The preparation of substrate of glass：Thick 1mm glass board materials are taken, and area are processed into for 10 × 18mm2Rectangle glass piece as glass
Substrate；
The preparation of iron based nano crystal substrate：Thick 20 μm iron based nano crystal band is pasted on the glass substrate, polish after solidification,
Minute surface is polished to, to form iron based nano crystal substrate；
The preparation of silvered substrates：Vacuum plating silver, forms SERS substrate on the glass substrate；Thickness of coating is 100nm.
9. the detection method of protein characteristic as claimed in claim 7 identification, it is characterised in that described the step of prepare print
Including：
3 μ l of sample to be tested are taken with sample presentation device, drop covers thick 130 μm of slides in the substrate surface, on drop, stand 1～
2min, loads the prototype test platform after liquid layer under coverslip is evenly distributed and is fixed test.
10. the detection method that protein characteristic as claimed in claim 7 is recognized, it is characterised in that the detection method is also wrapped
Include：
The print in light path is positioned before measurement, and set the default sampling time, with protein sample during ensureing measurement all the time
In static liquid.
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| CN202281774U (en) * | 2011-10-31 | 2012-06-20 | 上海裕隆生物科技有限公司 | Full-automatic reaction system |
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