CN106544387A - Intestines slag is the method that raw material produces feed oligopeptide - Google Patents
Intestines slag is the method that raw material produces feed oligopeptide Download PDFInfo
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- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
Intestines slag is the method that raw material produces feed oligopeptide, and it is related to feed production technology field；Its preparation method is：Intestines slag uses colloid mill defibrination, slurry Jing compound proteases and enzymolysis accelerator enzymolysis, Jing filter, concentrate, being spray-dried and to obtain feed oligopeptide after adding suitable quantity of water.The features such as with process is simple, few equipment investment, high income (＞ 90%), product quality excellent (more than oligomeric peptide content/total protein >=90%), three-waste free discharge, it is adapted to small intestine deep processing enterprise and promotes the use of.
Description
Technical field
The present invention relates to feed production technology field, and in particular to a kind of intestines slag is the side that raw material produces feed oligopeptide
Method.
Background technology
Contain many utilities in chitterlings, it can be common that casing, plum blossom intestines, liquaemin, dermatan sulfate, sulfuric acid list
(Jiang Yan etc., 2001), antibacterial peptide is extracted in enzymolysis product, and (2012) Wu Hongyun etc., is extracting above-mentioned work for esterase, arklemin etc.
Property composition after remaining solid content be referred to as intestines slag.In intestines slag, protein content is different and different from the technique of extraction of substance, typically exists
20%-40% (Jiang Yan, 2001).
Chitterlings processing enterprise of China is a lot, mainly processes casing and extracts heparin sodium crude, the annual intestines slag for producing
There are nearly million tons, this is good feed protein resource, if comprehensive utilization so as to which resource is fully used, and can subtract again
Few pollution to environment.
As bioactive ingredients are extracted from chitterlings, need to add in a certain amount of NaCl and NaOH, therefore intestines slag
Also contain a certain amount of NaCl and NaOH, therefore in the application of feed be subject to a definite limitation (Jiang Yan, 2001).
In intestines residue protein, main component is mucoprotein, and as molecular weight is larger, fowl poultry kind animal is inhaled to mucinous digestion
Receipts have certain difficulty, particularly oviparity and aquatic animal, as alimentary canal is relatively short, the mucoprotein digestion in intestines slag are inhaled
Yield is lower.
To overcome disadvantages mentioned above, need intestines residue protein is extracted and degraded, reduce NaCl and NaOH content in product,
The mucoprotein of macromolecular is made to become the relatively small peptides of molecular weight simultaneously.At present, extracting method mainly has two kinds of alkali carries and enzymolysis
Method.
The condition of alkaline extraction is：Solid-liquid ratio (g/ml) is 1: 15, and alkali concn is 0.5%, and Extracting temperature is 60 DEG C, during extraction
Between be 2h, the recovery rate of protein be 34.1% (to intestines slag) and 87.26% (to protein) (Kong Fanwei, 2011).
The enzymolysis trypsase of intestines slag, solid-liquid ratio is 1: 20, enzyme concentration 1.5%, and hydrolysis temperature is 50 DEG C, enzymolysis time
For 5h, and protein maximum extracted rate 26.32% (to intestines slag) and 67.34% (to protein) (Kong Fanwei, 2011).
The research to intestines slag is taken a broad view of, still has following deficiency：
1st, research intestines slag is drying, the intestines ground-slag of crushing, rather than with the wet intestines slag got off on production line, is so increased
Plus the energy resource consumption being dried, increase production cost；
2nd, intestines slag has no pretreatment, affects enzymolysis efficiency；
3rd, intestines slag enzymolysis enzyme has no and carries out screening system；
4th, only had no and used complex enzyme zymohydrolysis with single enzyme enzymolysis；
5th, digest yield low, only 67.34% (to protein).
6th, molecular weight determination is not carried out to enzymolysis product, therefore the digestibility of the product cannot be evaluated.
The content of the invention
It is an object of the invention to provide a kind of have process is simple, few equipment investment, high income (＞ 90%), product quality
The features such as excellent (more than oligomeric peptide content/total protein >=90%), three-waste free discharge, it is adapted to what small intestine deep processing enterprise promoted the use of
Intestines slag is the method that raw material produces feed oligopeptide.
In order to solve the problems of background technology, the present invention is to employ the following technical solutions：A kind of intestines slag is raw material
The method of production feed oligopeptide, its preparation method is：Intestines slag uses colloid mill defibrination, slurry Jing to be combined egg after adding suitable quantity of water
White enzyme and enzymolysis accelerator enzymolysis, Jing filter, concentrate, being spray-dried and to obtain feed oligopeptide.
The present invention concrete production technology be：
(1) wet intestines slag is added water allotment by 1: 5-15；
(2) allotment feed liquid is passed through into colloid mill defibrination；
(3) slurry water-bath is heated to 40 DEG C -60 DEG C；
(4) pH7.5-9.0 is adjusted with NaOH solution；
(5) add compound protease 1.0%-5.0% (total protein)；Compound protease is the base in the screening of single enzyme system
Compounded on plinth, its proportioning be 1.398 neutral proteinase 30%-60% of As, 2709 alkali protease 30%-60%, pancreatin
10%-40%；
(6) enzyme-added solution accelerator L-cysteine hydrochloride 0.01%-0.1%；Stirring enzymolysis 3h-6h；Flavouring liquid
pH5.0-7.0；70 DEG C -90 DEG C are warming up to, 10min-20min goes out enzyme；
(7) plate-frame filtering；
(8) clear liquid Jing vacuum decompressions are concentrated by evaporation to solid content 30%-60%；
(9) concentrate is spray-dried obtains feed intestines slag oligopeptide.
After using above-mentioned technical proposal, the invention has the advantages that：
1st, directly fed intake with wet intestines slag on production line, save the energy used by drying intestines slag, reduction producing cost increases product
Competitiveness；
2nd, after the allotment of intestines slag water, Jing colloid mills wear into slurry, make intestines solid impurity particle diminish, increase the contact surface with protease,
Make enzyme be easily accessible the specific site of amino acid combination in protein structure, improve enzymolysis yield；
3rd, enzymolysis compound protease, makes enzymolysis yield up to more than 80%, and product middle-molecular-weihydroxyethyl ＜ 2000Da parts account for total
Protein ratio is more than 80%；
4th, used enzymolysis accelerator L-cysteine hydrochloride, make enzymolysis yield improve more than 10%, reach 90% with
On；In product, the content of oligopeptide also improves more than 10%, reaches more than 90%；
5th, product yield is increased, improves product quality, reduce product cost, increase substantially product commercially
Competitiveness.
Description of the drawings
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
Accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 is the process chart of embodiment provided by the present invention.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, below in conjunction with accompanying drawing and it is embodied as
Mode, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only to explain this
Invention, is not intended to limit the present invention.
Specific embodiment one：Fig. 1 is referred to, this specific embodiment is employed the following technical solutions：A kind of intestines slag is original
The method of material production feed oligopeptide, its preparation method is：
A, intestines slag 50g is taken, add water 400ml, dispersed with stirring；
B, spice is put in wall-breaking machine breaks into slurry；
C, slurry is put in beaker, heating water bath is put into 40 DEG C ± 1 DEG C；
D, with 10%NaOH flavouring liquids pH7.5 ± 0.1；
E, plus compound protease 0.263g (2.5% Tot Prot), L-cysteine hydrochloride 0.08g (0.08%, w/
V), stirring enzymolysis 3.5h；
F, material liquid pH are adjusted to 5.5；
G, intensification are heated to 70 DEG C, and 20min goes out enzyme；
H, feed liquid 4000rpm centrifugation 10min；
The rotated evaporimeter of i, clear liquid is concentrated into solid content 35% or so；
J, concentrate Jing test-type spray drying tower spray dryings obtain feed grade oligopeptide.
This specific embodiment has few process is simple, equipment investment, high income (＞ 90%), product quality excellent (oligomeric
It is more than peptide content/total protein >=90%), three-waste free discharge the features such as, be adapted to small intestine deep processing enterprise promote the use of.
Specific embodiment two：The preparation method of this specific embodiment is：
I, intestines slag 10kg is taken, add water 100kg, dispersed with stirring；
II, spice is worn into into slurries by colloid mill；
III, slurries are squeezed in enzymatic vessel, be heated to 45 DEG C ± 1 DEG C；
IV, feed liquid adjust pH8.0 ± 0.1 with 10%NaOH；
V, plus compound protease 63.12g (3.0% Tot Prot), L-cysteine hydrochloride 55.0g (0.05%, w/
V), stirring enzymolysis 4h；
VI, material liquid pH are adjusted to 6.0；
VII, intensification are heated to 75 DEG C, and 15min goes out enzyme；
VIII, feed liquid Jing plate-frame filtering obtain the stillness of night；
IX, clear liquid Jing are evaporated to solid content 40% or so；
X, concentrate is spray-dried into obtain feed grade oligopeptide.
Specific embodiment three：The preparation method of this specific embodiment is：
A, intestines slag 100kg is taken, add water 1500L, dispersed with stirring；
B, spice is worn into into slurries by colloid mill；
C, slurries are squeezed in enzymatic vessel, be heated to 50 DEG C ± 1 DEG C；
D, feed liquid adjust pH8.5 ± 0.1 with 10%NaOH；
E, plus compound protease 1.052kg (5.0% Tot Prot), L-cysteine hydrochloride 1.6g (0.1%, w/v),
Stirring enzymolysis 5h；
F, material liquid pH are adjusted to 6.5；
G, feed liquid heat up and are heated to 80 DEG C, and 10min goes out enzyme；
H, feed liquid Jing plate-frame filtering obtain the stillness of night；
I, clear liquid Jing are evaporated to solid content 50% or so；
J, concentrate is spray-dried into obtain feed grade oligopeptide.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of spirit or essential attributes without departing substantially from the present invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit is required rather than described above is limited, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.Any reference in claim should not be considered as and limit involved claim.
Moreover, it will be appreciated that although this specification is been described by according to embodiment, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity
Using specification as an entirety, the technical scheme in each embodiment can also Jing it is appropriately combined, form those skilled in the art
Understandable other embodiment.
Claims (5)
1. intestines slag is the method that raw material produces feed oligopeptide, it is characterised in that its preparation method is：Intestines slag adds suitable quantity of water
Colloid mill defibrination, slurry Jing compound proteases and enzymolysis accelerator enzymolysis, Jing is used to filter, concentrate, being spray-dried and to obtain feed use afterwards
Oligopeptide.
2. intestines slag is the method that raw material produces feed oligopeptide, it is characterised in that its concrete production technology is：
(1) wet intestines slag is added water allotment by 1: 5-15；
(2) allotment feed liquid is passed through into colloid mill defibrination；
(3) slurry water-bath is heated to 40 DEG C -50 DEG C；
(4) pH7.5-9.0 is adjusted with NaOH solution；
(5) compound protease 1.0%-5.0%, total protein are added；Compound protease is on the basis of the screening of single enzyme system
Compounded, its proportioning be 1.398 neutral proteinase 30%-60% of As, 2709 alkali protease 30%-60%, pancreatin
10%-40%；
(6) enzyme-added solution accelerator L-cysteine hydrochloride 0.01%-0.1%；Stirring enzymolysis 3h-6h；Flavouring liquid pH5.0-
7.0；70 DEG C -90 DEG C are warming up to, 10min-20min goes out enzyme；
(7) plate-frame filtering；
(8) clear liquid Jing vacuum decompressions are concentrated by evaporation to solid content 30%-60%；
(9) concentrate is spray-dried obtains feed intestines slag oligopeptide.
3. intestines slag according to claim 2 is the method that raw material produces feed oligopeptide, it is characterised in that its preparation
Method is：
(a), intestines slag 50g is taken, add water 400ml, dispersed with stirring；
(b), spice is put in wall-breaking machine breaks into slurry；
(c), slurry is put in beaker, heating water bath is put into 40 DEG C ± 1 DEG C；
(d), with 10%NaOH flavouring liquids pH7.5 ± 0.1；
(e), add compound protease 0.263g, 2.5% Tot Prot；L-cysteine hydrochloride 0.08g, 0.08%, w/v, stir
Mix enzymolysis 3.5h；
F (), material liquid pH are adjusted to 5.5；
G (), intensification are heated to 70 DEG C, 20min goes out enzyme；
H (), feed liquid 4000rpm are centrifuged 10min；
I the rotated evaporimeter of (), clear liquid is concentrated into solid content 35% or so；
J (), concentrate Jing test-type spray drying tower spray dryings obtain feed grade oligopeptide.
4. intestines slag according to claim 2 is the method that raw material produces feed oligopeptide, it is characterised in that its preparation
Method is：
(I) intestines slag 10kg, is taken, add water 100kg, dispersed with stirring；
(II) spice is worn into into slurries by colloid mill,；
(III), slurries are squeezed in enzymatic vessel, 45 DEG C ± 1 DEG C is heated to；
(IV), feed liquid adjusts pH8.0 ± 0.1 with 10%NaOH；
(V), compound protease 63.12g, 3.0% Tot Prot are added；L-cysteine hydrochloride 55.0g, 0.05%, w/v, stir
Mix enzymolysis 4h；
(VI), material liquid pH is adjusted to 6.0；
(VII), heat up and be heated to 75 DEG C, 15min goes out enzyme；
(VIII), feed liquid Jing plate-frame filtering obtains the stillness of night；
(IX), clear liquid Jing is evaporated to solid content 40% or so；
(X) concentrate is spray-dried into obtain feed grade oligopeptide,.
5. intestines slag according to claim 2 is the method that raw material produces feed oligopeptide, it is characterised in that its preparation
Method is：
(A) intestines slag 100kg, is taken, add water 1500L, dispersed with stirring；
(B) spice is worn into into slurries by colloid mill,；
(C), slurries are squeezed in enzymatic vessel, 50 DEG C ± 1 DEG C is heated to；
(D), feed liquid adjusts pH8.5 ± 0.1 with 10%NaOH；
(E), compound protease 1.052kg, 5.0% Tot Prot are added；L-cysteine hydrochloride 1.6g, 0.1%, w/v, stirring
Enzymolysis 5h；
(F), material liquid pH is adjusted to 6.5；
(G), feed liquid heats up and is heated to 80 DEG C, and 10min goes out enzyme；
(H), feed liquid Jing plate-frame filtering obtains the stillness of night；
(I), clear liquid Jing is evaporated to solid content 50% or so；
(J) concentrate is spray-dried into obtain feed grade oligopeptide,.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321716A (en) * | 2011-08-18 | 2012-01-18 | 江中药业股份有限公司 | Preparation method of donkey-hide gelatin oligopeptide |
| CN104351457A (en) * | 2014-11-19 | 2015-02-18 | 乳山市华隆生物科技有限公司 | Fish scale oligopeptide powder and preparation method thereof |
| CN105154505A (en) * | 2015-09-21 | 2015-12-16 | 常熟理工学院 | Preparation method of feed grade ocean fish oligopeptide meal |
-
2016
- 2016-10-26 CN CN201610922533.4A patent/CN106544387A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321716A (en) * | 2011-08-18 | 2012-01-18 | 江中药业股份有限公司 | Preparation method of donkey-hide gelatin oligopeptide |
| CN104351457A (en) * | 2014-11-19 | 2015-02-18 | 乳山市华隆生物科技有限公司 | Fish scale oligopeptide powder and preparation method thereof |
| CN105154505A (en) * | 2015-09-21 | 2015-12-16 | 常熟理工学院 | Preparation method of feed grade ocean fish oligopeptide meal |
Non-Patent Citations (3)
| Title |
|---|
| 叶元土: "《水产集约化健康养殖技术》", 31 January 2007, 中国农业出版社 * |
| 孔凡伟等: "酶法提取肠渣饲用蛋白质工艺的研究", 《粮食与饲料工业》 * |
| 窦屾: "酶解大豆粉蛋白条件优化研究", 《北京工商大学学报 (自然科学版 )》 * |
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Application publication date: 20170329 |