CN106543023B - 茶老叶中茶多酚和茶氨酸的提取方法及茶叶渣的处理方法 - Google Patents
茶老叶中茶多酚和茶氨酸的提取方法及茶叶渣的处理方法 Download PDFInfo
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- CN106543023B CN106543023B CN201610962795.3A CN201610962795A CN106543023B CN 106543023 B CN106543023 B CN 106543023B CN 201610962795 A CN201610962795 A CN 201610962795A CN 106543023 B CN106543023 B CN 106543023B
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- Prior art keywords
- tea
- old leaf
- theanine
- tea old
- polyphenols
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Links
- 241001122767 Theaceae Species 0.000 title claims abstract description 141
- DATAGRPVKZEWHA-YFKPBYRVSA-N N(5)-ethyl-L-glutamine Chemical compound CCNC(=O)CC[C@H]([NH3+])C([O-])=O DATAGRPVKZEWHA-YFKPBYRVSA-N 0.000 title claims abstract description 88
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 45
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 45
- 229940026510 theanine Drugs 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000003672 processing method Methods 0.000 title description 2
- 239000002893 slag Substances 0.000 claims abstract description 27
- 238000002386 leaching Methods 0.000 claims abstract description 20
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000011347 resin Substances 0.000 claims abstract description 7
- 229920005989 resin Polymers 0.000 claims abstract description 7
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 239000003480 eluent Substances 0.000 claims description 18
- 235000019441 ethanol Nutrition 0.000 claims description 14
- 239000000243 solution Substances 0.000 claims description 14
- 239000007921 spray Substances 0.000 claims description 14
- 238000001179 sorption measurement Methods 0.000 claims description 12
- 239000012153 distilled water Substances 0.000 claims description 10
- 150000001768 cations Chemical class 0.000 claims description 6
- 239000007979 citrate buffer Substances 0.000 claims description 6
- 238000002425 crystallisation Methods 0.000 claims description 6
- 230000008025 crystallization Effects 0.000 claims description 6
- 239000003456 ion exchange resin Substances 0.000 claims description 6
- 229920003303 ion-exchange polymer Polymers 0.000 claims description 6
- 238000011084 recovery Methods 0.000 claims description 6
- 108010059892 Cellulase Proteins 0.000 claims description 5
- 229940106157 cellulase Drugs 0.000 claims description 5
- 238000007654 immersion Methods 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 4
- 238000010828 elution Methods 0.000 claims description 2
- 235000009024 Ceanothus sanguineus Nutrition 0.000 abstract description 20
- 240000003553 Leptospermum scoparium Species 0.000 abstract description 20
- 235000015459 Lycium barbarum Nutrition 0.000 abstract description 20
- 238000000855 fermentation Methods 0.000 abstract description 14
- 230000004151 fermentation Effects 0.000 abstract description 14
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 abstract description 12
- 239000005696 Diammonium phosphate Substances 0.000 abstract description 10
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 abstract description 10
- 229910000388 diammonium phosphate Inorganic materials 0.000 abstract description 10
- 235000019838 diammonium phosphate Nutrition 0.000 abstract description 10
- 241000193375 Bacillus alcalophilus Species 0.000 abstract description 6
- 241000499912 Trichoderma reesei Species 0.000 abstract description 6
- 238000000605 extraction Methods 0.000 abstract description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract description 6
- 235000011164 potassium chloride Nutrition 0.000 abstract description 6
- 239000001103 potassium chloride Substances 0.000 abstract description 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 abstract description 5
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 abstract description 5
- 229910000389 calcium phosphate Inorganic materials 0.000 abstract description 5
- 239000004202 carbamide Substances 0.000 abstract description 5
- 235000003891 ferrous sulphate Nutrition 0.000 abstract description 5
- 239000011790 ferrous sulphate Substances 0.000 abstract description 5
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 abstract description 5
- 229910000359 iron(II) sulfate Inorganic materials 0.000 abstract description 5
- 235000007079 manganese sulphate Nutrition 0.000 abstract description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 abstract description 5
- 235000019691 monocalcium phosphate Nutrition 0.000 abstract description 5
- 239000002994 raw material Substances 0.000 abstract description 5
- 239000000284 extract Substances 0.000 abstract description 4
- 239000003337 fertilizer Substances 0.000 abstract description 4
- 238000002360 preparation method Methods 0.000 abstract description 3
- 235000018660 ammonium molybdate Nutrition 0.000 abstract description 2
- 229940099596 manganese sulfate Drugs 0.000 abstract description 2
- 239000011702 manganese sulphate Substances 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 abstract 1
- 239000011609 ammonium molybdate Substances 0.000 abstract 1
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 abstract 1
- 229940010552 ammonium molybdate Drugs 0.000 abstract 1
- 239000003729 cation exchange resin Substances 0.000 abstract 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 abstract 1
- 238000005469 granulation Methods 0.000 abstract 1
- 230000003179 granulation Effects 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 239000002699 waste material Substances 0.000 abstract 1
- 238000009434 installation Methods 0.000 description 20
- 210000003608 fece Anatomy 0.000 description 16
- 239000010871 livestock manure Substances 0.000 description 16
- 241000233866 Fungi Species 0.000 description 9
- 238000003756 stirring Methods 0.000 description 8
- OHZCFWMJMWFNFP-ZVGUSBNCSA-L (2r,3r)-2,3-dihydroxybutanedioate;iron(2+) Chemical compound [Fe+2].[O-]C(=O)[C@H](O)[C@@H](O)C([O-])=O OHZCFWMJMWFNFP-ZVGUSBNCSA-L 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 4
- 229940057006 ferrous tartrate Drugs 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 238000004811 liquid chromatography Methods 0.000 description 4
- 238000009630 liquid culture Methods 0.000 description 4
- 230000007774 longterm Effects 0.000 description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical class [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 4
- 238000009966 trimming Methods 0.000 description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 235000016768 molybdenum Nutrition 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000013138 pruning Methods 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C237/00—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups
- C07C237/02—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups having the carbon atoms of the carboxamide groups bound to acyclic carbon atoms of the carbon skeleton
- C07C237/04—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups having the carbon atoms of the carboxamide groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being acyclic and saturated
- C07C237/06—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups having the carbon atoms of the carboxamide groups bound to acyclic carbon atoms of the carbon skeleton the carbon skeleton being acyclic and saturated having the nitrogen atoms of the carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C231/00—Preparation of carboxylic acid amides
- C07C231/22—Separation; Purification; Stabilisation; Use of additives
- C07C231/24—Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07G—COMPOUNDS OF UNKNOWN CONSTITUTION
- C07G99/00—Subject matter not provided for in other groups of this subclass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Fertilizers (AREA)
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CN201610962795.3A CN106543023B (zh) | 2016-11-04 | 2016-11-04 | 茶老叶中茶多酚和茶氨酸的提取方法及茶叶渣的处理方法 |
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CN201610962795.3A CN106543023B (zh) | 2016-11-04 | 2016-11-04 | 茶老叶中茶多酚和茶氨酸的提取方法及茶叶渣的处理方法 |
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CN108517292B (zh) * | 2018-04-27 | 2019-05-10 | 金威宝(广东)健康产业有限公司 | 一种自动从茶叶中提取茶氨酸提取设备及使用方法 |
CN108634311A (zh) * | 2018-05-08 | 2018-10-12 | 紫阳县硒资源开发研究所 | 一种提取茶叶活性物质的工艺方法 |
CN114507623B (zh) * | 2022-03-04 | 2023-03-10 | 安徽农业大学 | 一种芽孢杆菌及其应用 |
CN115918902A (zh) * | 2022-12-19 | 2023-04-07 | 新疆农业科学院农业质量标准与检测技术研究所 | 一种通过微水波扩散重力法预处理葡萄脱水并获取高酚葡萄提取物的方法 |
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JP5435585B2 (ja) * | 2009-09-24 | 2014-03-05 | 万壽美 竹元 | 穀粉食品及び穀粉食品の製造方法 |
CN102675317B (zh) * | 2012-05-29 | 2014-08-27 | 福建省安溪县南洋茶厂 | 一种从茶叶中同时提取多种有效成分的方法 |
KR102024571B1 (ko) * | 2012-11-15 | 2019-09-24 | (주)아모레퍼시픽 | 서방성 녹차 세포 전달체 및 이를 함유하는 화장료 조성물 |
CN106064967A (zh) * | 2016-05-30 | 2016-11-02 | 大连三科生物工程有限公司 | 一种根茎类蔬菜专用的有机复合生物肥的制备方法 |
CN106045677A (zh) * | 2016-05-30 | 2016-10-26 | 大连三科生物工程有限公司 | 一种果树专用的有机复合生物肥的制备方法 |
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Address after: 443200 Hubei temple town city in Yichang province Zhijiang City Anfu word Creek Road Patentee after: Hubei Yulong Bioengineering Co.,Ltd. Address before: 443200 Hubei temple town city in Yichang province Zhijiang City Anfu word Creek Road Patentee before: HUBEI YU LONG TEA INDUSTRY Co.,Ltd. |
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Denomination of invention: Extraction method of tea polyphenols and theanine from old tea leaves and treatment method of tea residue Effective date of registration: 20230516 Granted publication date: 20190412 Pledgee: Zhijiang Jinqiao Financing Guarantee Co.,Ltd. Pledgor: Hubei Yulong Bioengineering Co.,Ltd. Registration number: Y2023980040738 |
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Denomination of invention: Extraction methods of tea polyphenols and theanine from old tea leaves and treatment methods of tea residue Granted publication date: 20190412 Pledgee: Zhijiang Jinqiao Financing Guarantee Co.,Ltd. Pledgor: Hubei Yulong Bioengineering Co.,Ltd. Registration number: Y2024980017274 |
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