CN106532426A - Enhancing device for multiphoton imaging signal - Google Patents
Enhancing device for multiphoton imaging signal Download PDFInfo
- Publication number
- CN106532426A CN106532426A CN201710013169.4A CN201710013169A CN106532426A CN 106532426 A CN106532426 A CN 106532426A CN 201710013169 A CN201710013169 A CN 201710013169A CN 106532426 A CN106532426 A CN 106532426A
- Authority
- CN
- China
- Prior art keywords
- orphan
- incident
- pumping pulse
- pumping
- pulse
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01S—DEVICES USING THE PROCESS OF LIGHT AMPLIFICATION BY STIMULATED EMISSION OF RADIATION [LASER] TO AMPLIFY OR GENERATE LIGHT; DEVICES USING STIMULATED EMISSION OF ELECTROMAGNETIC RADIATION IN WAVE RANGES OTHER THAN OPTICAL
- H01S3/00—Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range
- H01S3/30—Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range using scattering effects, e.g. stimulated Brillouin or Raman effects
- H01S3/302—Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range using scattering effects, e.g. stimulated Brillouin or Raman effects in an optical fibre
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
Landscapes
- Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Electromagnetism (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Plasma & Fusion (AREA)
- Optical Modulation, Optical Deflection, Nonlinear Optics, Optical Demodulation, Optical Logic Elements (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention is applicable to the technical field of optical imaging, and provides an enhancing device for a multiphoton imaging signal. The enhancing device comprises a pulse generating unit, an orphan generating device and an orphan filtering unit, wherein the pulse generating unit is used for generating a pumping pulse with preset repetition frequency and pulse width to be incident to the orphan generating unit; the orphan generating unit is used for adjusting the incident pumping pulse to be at a preset polarization state and carrying out polarization multiplexing treatment, generating an orphan self-frequency shift phenomenon to obtain two high-energy orphan beams with same wavelength and cross polarization, and the enabling the two high-energy orphan beams to be incident to the orphan filtering unit; the orphan filtering unit is used for carrying out filtering treatment on the two incident high-energy orphan beams and obtaining an orphan with a preset wavelength as an excited signal to be sent to a laser scanning microscope system so that the laser scanning microscope system carries out multiphoton imaging according to the excited signal. Through the enhancing device provided by the invention, the repetition frequency of the orphan can be improved by using a polarization multiplexing technology, so that the signal level of the multiphoton imaging signal is improved.
Description
Technical field
The invention belongs to optical image technology field, more particularly to a kind of intensifier of multi-photon imaging signal.
Background technology
Soliton self-frequency sh is the nonlinear optical effect based on stimulated Raman scattering in pulse, is that one kind can produce wavelength
The effective ways of tunable femtosecond pulse.Many fiber waveguides (mainly optical fiber) with anomalous dispersion may serve to produce orphan
Sub- self-frequency shift, such as standard single-mode fiber, refractive-index-guiding photonic crystal fiber, hollow core photonic bandgap fiber, large mode field optical fiber
With the photonic crystal rod of large mode field.It is based primarily upon standard single-mode fiber (such as fused quartz light of optical material natural dispersion characteristic
Fine and fluoride fiber) can produce and receive the orphan of burnt magnitude energy.Refractive-index-guiding photonic crystal fiber can be customized to appoint
The zero-dispersion wavelength of meaning, therefore the orphan for producing can cover broader wave band, but orphan's energy is only capable of reaching Ya Najiao magnitudes.
Hollow core photonic bandgap fiber can also be customized to arbitrary zero-dispersion wavelength, and orphan's energy of generation can be up to micro- burnt magnitude,
But it is close to passband edge decay to significantly increase, therefore orphan's wavelength tuning range only has tens nanometers.And large mode field optical fiber and
The characteristics of photonic crystal rod has broad band wavelength tuning range (hundreds of nanometer) and high soliton pulse energy (receive more than 100 Jiao) concurrently,
But the wavelength for being limited its orphan by the material dispersion of fused quartz can only be more than 1.3 microns.
Multi-photon imaging needs the femto-second laser pulse of tunable wave length as excitation source, and soliton self-frequency sh technology becomes
One of ideal chose of multi-photon imaging.1700 nano waveband high energy can be obtained using the soliton self-frequency sh in photonic crystal rod
The orphan of amount, using the soliton lamp -house as excitation source, and the three-photon micro-imaging technique that background suppresses, can live
Body mouse brain obtains maximum imaging depth, so as to clearly represent subcortical structure.Before the poster presentation, accomplish
The technology that the multi-photon imaging of this depth needs using very invasive, such as removes tissue grey matter above or past brain
The technology of insertion endoscope.
The applicant has found that so far, the imaging depth of 1700 nano wavebands is (about in the practice of the invention
At 1.4 millimeters) mainly by deep tissues in the signal level that produces limited, therefore how more effectively to strengthen signal level
It is the key for improving imaging depth.From the angle of excitation source, following two technologies are mainly adopted at present：(1) using more
The photonic crystal rod of big core size carries out soliton self-frequency sh.Because orphan's energy is directly proportional with effective core area, fibre core chi
Very little bigger, orphan's energy is higher.Two-photon and three-photon signal can be by formula Ss2∝E2F/ τ and S3∝E3f/τ2Draw, its
In, E represents pulse energy, and f represents pulse recurrence frequency, and τ represents pulsewidth.It can be seen that can be strengthened by core size
Signal.(2) dispersion compensation, that is, Pulse Width of Soliton on sample is compressed to minimum of a value.Signal level is understood according to above-mentioned formula
Also therefore can improve.
Improve core size and eventually sustained damage the restriction of threshold value with obtaining bigger this technology of orphan's energy.Peak value
Bulk damage effect and the self-focusing effect for exceeding fused quartz optical fiber self-focusing threshold limit value (about 4 megawatts) and producing that light intensity is caused
Should be likely to cause damage photonic crystal rod or optical fiber.
The content of the invention
The technical problem to be solved is to provide a kind of intensifier of multi-photon imaging signal, it is desirable to provide
Improve the signal level of multi-photon imaging signal.
The present invention is achieved in that a kind of intensifier of multi-photon imaging signal, including impulse generating unit, orphan
Generation unit and orphan's filter unit：
The impulse generating unit, for producing the pumping pulse of preset repetition rate and pulsewidth, and by the pumping arteries and veins
Pour and be incident upon orphan's generation unit；
Orphan's generation unit, for the incident pumping pulse is adjusted to after the pumping pulse of preset polarization state
Carry out palarization multiplexing process, and according to the pumping pulse after process produce soliton self-frequency sh phenomenon obtain two beam phase co-wavelengths and
The high-energy orphan of cross-polarization, and high-energy orphan described in two beams is incident to into orphan's filter unit；
Orphan's filter unit, for being filtered process to two incident beam high-energy orphans, obtains preset wavelength
Orphan, and the orphan after process is sent to into laser scanning microscope system as excitation signal, so that the laser scanning is aobvious
Micro-system carries out multi-photon imaging according to the excitation signal.
Further, the impulse generating unit includes 1550 nano optical fibers laser instruments and the first silver mirror；
The 1550 nano optical fibers laser instrument, for producing the pumping arteries and veins of 1 MHz repetition and 500 femtosecond pulsewidths
Punching, and the pumping pulse is incident to into first silver mirror；
First silver mirror reflects incident pumping pulse into orphan's generation unit.
Further, orphan's generation unit includes pulse adjusting module and orphan's generation module；
The pulse adjusting module, for the incident pumping pulse is adjusted to into preset polarization state pumping pulse simultaneously
Palarization multiplexing process is carried out, the pumping pulse after process is incident to into orphan's generation module then；
Orphan's generation module, it is existing for producing soliton self-frequency sh according to the pumping pulse after the incident process
As obtaining the high-energy orphan of two beam phase co-wavelengths and cross-polarization, and high-energy orphan described in two beams being incident to the orphan
Sub- filter unit.
Further, the pulse adjusting module include the first half-wave plate, the second silver mirror, the 3rd silver mirror, the 4th silver mirror,
One polarizing beam splitter cube, the second polarizing beam splitter cube and the first lens；
First half-wave plate is used for the polarization state of the pumping pulse for adjusting incidence, and by the pumping pulse after adjustment
It is incident to second silver mirror；
Second silver mirror is reflected into first polarization beam splitting for the pumping pulse after incident polarization state is adjusted
Cube；
Incident pumping pulse is divided into the first pumping subpulse and the second pumping by first polarizing beam splitter cube
Pulse, and the second pumping subpulse is incident to into second polarizing beam splitter cube；
The first pumping subpulse is after described in, the 3rd silver mirror and the 4th silver mirror carry out light path adjustment respectively, with described
Two pumping subpulse vertical incidence are to second polarizing beam splitter cube；
Second polarizing beam splitter cube is for the first incident pumping subpulse and the second pumping subpulse are carried out
Beam is closed, the pumping pulse of 1550 nanometers of cross-polarizations of conllinear transmission is produced, and it is orthogonal by 1550 nanometers of the conllinear transmission
The pumping pulse of polarization is incident to first lens；
First lens are for carrying out to the pumping pulse of 1550 nanometers of cross-polarizations of incident described conllinear transmission
Focus on, and the pumping pulse after focusing is incident to into orphan's generation module.
Further, orphan's generation module is the sub- crystal bar of non-guarantor's polarisation that core diameter is 100 microns.
Further, orphan's generation unit also includes that light path adjusting module and orphan occur module；
The light path adjusting module, for the incident pumping pulse to be adjusted to the pumping pulse of preset polarization state,
And the pumping pulse after polarization state is adjusted is incident to the orphan and module occurs；
There is module in the orphan, for carrying out palarization multiplexing process to the pumping pulse after incident polarization state adjustment,
Then soliton self-frequency sh phenomenon is produced according to the pumping pulse after process, obtains the high energy of two beam phase co-wavelengths and cross-polarization
Amount orphan, and high-energy orphan described in two beams is incident to into orphan's filter unit.
Further, the light path adjusting module includes the second half-wave plate, the 5th silver mirror and the second lens；
Second half-wave plate is used for polarization state of the adjustment by the incident pumping pulse of the impulse generating unit, and
Pumping pulse after adjustment is incident to into the 5th silver mirror；
5th silver mirror is reflected into second lens for the pumping pulse after incident polarization state is adjusted；
Second lens are for being focused to incident pumping pulse, and the pumping pulse after focusing is incident to institute
State orphan and module occurs.
Further, there is module for guarantor's mould field optical fiber bigger than normal that core diameter is 40 microns in the orphan.
Further, orphan's filter unit includes the 3rd lens and optical filter；
3rd lens, for two beam soliton pulses of incident diverging are collimated so that the two beams orphan
It is parallel to be incident to the optical filter；
The optical filter, for being filtered process to the parallel orphan, obtains the orphan of preset wavelength, and will filter
The orphan obtained after ripple process is sent to laser scanning microscope system as excitation signal.
Further, the optical filter is long wave pass filter.
Compared with prior art, beneficial effect is the present invention：The embodiment of the present invention by produce preset repetition rate and
The pumping pulse of pulsewidth, and polarization state adjustment is carried out to the pumping pulse, will carry out the pumping pulse after polarization state adjustment is carried out
Palarization multiplexing process, and because soliton self-frequency sh phenomenon obtains two beam orphans of phase co-wavelength and cross-polarization, to obtain two
Beam orphan is filtered process, and the orphan after process is sent to laser scanning microscope system as excitation signal, sweeps laser
Retouching microscopic system carries out multi-photon imaging according to the excitation signal.By intensifier provided in an embodiment of the present invention, can
Increase the repetition rate of orphan so as to improve the signal level of multi-photon imaging signal using polarization multiplexing.
Description of the drawings
Fig. 1 is a kind of structural representation of the intensifier of multi-photon imaging signal that first embodiment of the invention is provided；
Fig. 2 is that a kind of detailed construction of the intensifier of multi-photon imaging signal that second embodiment of the invention is provided is illustrated
Figure；
In the sub- crystal bar of non-guarantor's polarisation that Fig. 3 is provided for second embodiment of the invention, horizontal state of polarization and polarization and vertical polarization are lonely
The measure spectrum and interference auto-correlation mark of son；
Fig. 4 is the three-photon fluorescent image in the sub- crystal bar of non-guarantor's polarisation that second embodiment of the invention is provided；
Fig. 5 is that a kind of detailed construction of the intensifier of multi-photon imaging signal that third embodiment of the invention is provided is illustrated
Figure；
Fig. 6 is horizontal state of polarization and polarization and vertical polarization orphan in guarantor's mould field optical fiber bigger than normal that third embodiment of the invention is provided
Measure spectrum and interfere auto-correlation mark；
Fig. 7 is the two-photon fluorescence imaging figure in mould field optical fiber bigger than normal is protected that third embodiment of the invention is provided.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, it is below in conjunction with drawings and Examples, right
The present invention is further elaborated.It should be appreciated that specific embodiment described herein is only to explain the present invention, and
It is not used in the restriction present invention.
Applicant of the present invention has found during the embodiment present invention, improves core size to obtain bigger orphan's energy
Measure this technology eventually to sustain damage the restriction of threshold value, once orphan's ceiling capacity is damaged threshold value limited, Pulse Width of Soliton
Also due to dispersion compensation and reached minimum of a value, it is desirable to strengthen multi-photon imaging signal, unique controllable laser parameter is exactly to weigh
Complex frequency, because multi-photon imaging signal is proportional to repetition rate, the increase of repetition rate can also effectively improve light more
The signal level of subsignal, based on this, the invention provides a kind of intensifier of multi-photon imaging signal as shown in Figure 1,
Including impulse generating unit 1, orphan's generation unit 2, and orphan's filter unit 3：
Impulse generating unit 1, for producing the pumping pulse of preset repetition rate and pulsewidth, and the pumping pulse is entered
It is incident upon orphan's generation unit 2；
Orphan's generation unit 2, the pumping pulse for the incident pumping pulse to be adjusted to preset polarization state are gone forward side by side
The process of row palarization multiplexing, and soliton self-frequency sh phenomenon is produced according to the pumping pulse after process obtain two beam phase co-wavelengths and just
The high-energy orphan for handing over polarization, and high-energy orphan described in two beams is incident to into orphan's filter unit 3.
Orphan's filter unit 3, for being filtered process to two incident beam high-energy orphans, obtains the orphan of preset wavelength
Son, and the orphan after process is sent to into laser scanning microscope system as excitation signal, so that the scan laser microphotograph system
System carries out multi-photon imaging according to the excitation signal.
By intensifier provided in an embodiment of the present invention, can using polarization multiplexing increase orphan's repetition rate from
And improve the signal level of multi-photon imaging signal.In the present embodiment, intensifier utilizes polarization multiplexing, two beams just
The pulse of polarization is handed over spatially to be combined (but due to postponing so being separate on time), so relative to a branch of arteries and veins
Bring repetition rate and become twice, and the two pulses of cross-polarization produce the orphan of two beam cross-polarizations because of soliton self-frequency sh
Son, is spatially combined.
On the basis of first embodiment, the present invention provides the second embodiment shown in Fig. 2, wherein：Impulse generating unit 1
Including 1550 nano optical fibers laser instrument Fiber Laser and the first silver mirror M1；1550 nano optical fibers laser instrument Fiber Laser,
For producing the pumping pulse of 1 MHz repetition and 500 femtosecond pulsewidths, and the pumping pulse is incident to into the first silver mirror
M1, the pumping pulse are 1550 nano wire polarized pump pulses；After incident pumping pulse is reflected by the first silver mirror M1,
It is incident to orphan's generation unit 2.
Orphan's generation unit 2 includes pulse adjusting module 21 and orphan's generation module 22；
Pulse adjusting module 21, the pumping pulse for the incident pumping pulse to be adjusted to preset polarization state are gone forward side by side
Then pumping pulse after process is incident to orphan's generation module 22 by the process of row palarization multiplexing；
Orphan's generation module 22, for producing soliton self-frequency sh phenomenon according to the pumping pulse after the incident process,
The high-energy orphan of two beam phase co-wavelengths and cross-polarization is obtained, and high-energy orphan described in two beams is incident to into orphan's filtering
Unit 3.
Pulse adjusting module 21 include the first half-wave plate HWP1, the second silver mirror M2, the 3rd silver mirror M3, the 4th silver mirror M4,
One polarizing beam splitter cube PBS1, the second polarizing beam splitter cube PBS2 and the first lens L1；
First half-wave plate HWP1 is used for the polarization state of the pumping pulse for adjusting incidence, and by the pumping pulse after adjustment
It is incident to the second silver mirror M2；Pumping pulse after polarization state adjustment is incident to the first polarization beam splitting Jing after the second silver mirror M2 reflections
Cube PBS1；Incident pumping pulse is divided into the first pumping subpulse and the second pumping by the first polarization beam splitting solid side PBS1
Subpulse, and the second pumping subpulse is incident to into the second polarizing beam splitter cube PBS2；The first pumping subpulse is distinguished
Jing after the 3rd silver mirror M3 and the 4th silver mirror M4 carry out light path adjustment, with the second pumping subpulse vertical incidence to the second polarization point
Beam cube PBS2；Second polarizing beam splitter cube PBS2 is for by the first incident pumping subpulse and the second pumping subpulse
Conjunction beam is carried out, the pumping pulse of 1550 nanometers of cross-polarizations of conllinear transmission is produced, and by 1550 nanometers of the conllinear transmission
The pumping pulse of cross-polarization is incident to the first lens L1；First lens L1 is for receiving to the 1550 of incident described conllinear transmission
The pumping pulse of rice cross-polarization is focused, and the pumping pulse after focusing is incident to orphan's generation module 22.
In a particular application, orphan's generation module 22 is the sub- crystal bar PC of non-guarantor's polarisation that core diameter is 100 microns
Rod, the non-guarantor sub- crystal bar PC rod of polarisation, for there is soliton self-frequency sh according to the pumping pulse after the incident process
Phenomenon, produces the high-energy orphan of two beam phase co-wavelengths and cross-polarization, and high-energy orphan described in two beams is incident to described
Orphan's filter unit 3.
Orphan's filter unit 3 includes the 3rd lens L3 and optical filter LPF；3rd lens L3, for by incident diverging
Two beam orphans are collimated so that the two beams orphan is parallel to be incident to optical filter LPF；Optical filter LPF, for described parallel
Orphan be filtered process, obtain the orphan of preset wavelength, and send out the orphan obtained after filtering process as excitation signal
Give laser scanning microscope system LSM.Specifically, optical filter LPF is long wave pass filter.
In order to improve the signal level of multi-photon imaging, provide one kind in the present embodiment using polarization multiplexing to increase
The intensifier of soliton pulse repetition rate.Polarization multiplexing be usually for double-colored soliton is produced in polarization maintaining optical fibre,
Its physical principle is：The linear polarization pumping pulse light of input can be divided into the pumping pulse light of two beam cross-polarizations, and with difference
Speed propagate respectively along two main shafts of optical fiber.This two beams pumping pulse light all can be produced due to soliton self-frequency sh phenomenon
Orphan.The polarization state of adjustment input line polarized pump pulsed light constantly can be adjusted between the wavelength of two beam cross-polarization orphans
Every.In the present embodiment, polarization is applied again in the intensifier of the sub- crystal bar of non-guarantor's polarisation of no polarization structure is included
With technology, phase co-wavelength, the high-energy orphan of cross-polarization can be equally generated.On this basis, the present embodiment will be polarized
Multiplex technique has been applied to three-photon and two photon imaging.Through test result indicate that, excited by polarization coupling light and obtained most
Whole signal level excites the signal sum for obtaining respectively equal to two beam cross-polarization orphans.It is therefore seen that, in multi-photon imaging,
Polarization multiplexing can improve signal level.
In the present embodiment, 1550 nano optical fibers laser instruments of 1 MHz repetition and 500 femtosecond pulsewidths are employed
(FLCPA-02CSZU, Calmar), used as pump light source, which produces the pumping arteries and veins of 1 MHz repetition and 500 femtosecond pulsewidths
Punching, the pumping pulse are 1550 nano wire polarized pump pulses.First half-wave plate HWP is for changing 1550 nano wire polarized pumps
The polarization state of Pu pulse, and with this adjusting the energy proportioning along two orthogonal polarization orientations.Non- guarantor employed in which is inclined
The core diameter of photonic crystal rod is 100 microns, and length is 44 centimetres (SC-1500/100-Si-ROD, NKT Photonics).
This non-sub- crystal bar of polarisation of protecting is not protect inclined structure, even if but the polarization state through 1 meter of conveying length laser can also
Remain unchanged.Two polarizing beam splitter cubes are used for producing 1550 nanometers of cross-polarization pumping pulses of conllinear transmission.This 1550
Nanometer cross-polarization pumping pulse through producing soliton self-frequency sh in non-guarantor's sub- crystal bar of polarisation, then through long wave pass filter
The orphan required for the present embodiment has been obtained afterwards.The present embodiment additionally uses spectrometer (OSA203B) and takes for the present embodiment
The interference autocorrelation function analyzer built comes measure spectrum and pulsewidth.Orphan after eventually passing spectrometer and interfering autocorrelation function analyzer is swept in laser
Multi-photon imaging is carried out in retouching microscopic system (LSM).
In the present embodiment, polarization multiplexing is applied in the sub- crystal bar of non-guarantor's polarisation first.Fig. 3 shows filter
The spectrum of horizontal state of polarization orphan (a in figure three) and polarization and vertical polarization orphan (b in Fig. 3) and interference auto-correlation mark after ripple.
Under the conditions of maximum pump energy, two orphans are moved to 1620 nanometer wave strong points, orphan's energy be respectively 73 receive it is burnt and
75 receive Jiao.Horizontal state of polarization and the interference auto-correlation mark of polarization and vertical polarization orphan that c and d in Fig. 3 shows respectively, it is possible thereby to
Obtain pulsewidth.It is assumed that orphan is hyperbolic secant model, after deconvolution is calculated, measure the pulsewidth for obtaining be respectively 73 femtoseconds and
70 femtoseconds.
It is conllinear propagation that one major advantage of polarization multiplexing is two orphans, and this is for multi-photon imaging
With very big benefit, because the space coincidence of two beam exciting lights is most important to multi-photon imaging.In this embodiment, to quilt
The intravital mouse blood vessel of Texas-red dye markers carries out three-photon fluorescent imaging, and the letter of polarization multiplexing is proved with this
Number humidification.In order to quantify this signal enhancing, the average three-photon fluorescent signal that compared in same angiosomes is strong
Degree, as shown in the marked region of Fig. 4, in Fig. 4, colourity bar indicates signal level, and scale is 50 microns.Horizontal state of polarization and perpendicular
Straight polarization state orphan is due to similar energy and pulsewidth, so the signal value for producing also approximately equal, respectively 28390 Hes
27970.When two orphans are incided and carry out three-photon fluorescent on sample and excite simultaneously, signal value is 56980, substantially equal to two
Signal value sum when individual orphan excites respectively.Therefore, the non-polarization multiplexing protected in the sub- crystal bar of polarisation can be effectively
Improve multi-photon imaging signal.It is further to note that before the sub- crystal bar of non-guarantor's polarisation, being polarized by two 1550 nanometers
The transmitance of the polarisation multiplex system of beam-dividing cube and two silver mirror compositions is up to 94%.
In practical application, also exist and protect the sub- crystal bar of polarisation.Polarization multiplexing in this large mode field fiber waveguide is more
Easily go to realize.In order to imitate the sub- crystal bar of this guarantor's polarisation, present invention also offers another as shown in Figure 5 is implemented
The concrete structure of example, impulse generating unit 1 and orphan's filter unit 3 is identical with the second embodiment of above-mentioned offer, simply in orphan
The concrete structure of sub- generation unit 2 is different, wherein：
Orphan's generation unit 2 also includes that light path adjusting module 23 and orphan occur module 24；
Light path adjusting module 23, for the incident pumping pulse to be adjusted to the pumping pulse of preset polarization state, and
Pumping pulse after polarization state is adjusted is incident to orphan and module 24 occurs；
There is module 24 in orphan, for carrying out palarization multiplexing process to the pumping pulse after incident polarization state adjustment, so
Soliton self-frequency sh phenomenon is produced according to the pumping pulse after process afterwards, the high-energy of two beam phase co-wavelengths and cross-polarization is obtained
Orphan, and high-energy orphan described in two beams is incident to into orphan's filter unit 3.
Light path adjusting module 23 includes the second half-wave plate HWP2, the 5th silver mirror M5 and the second lens L2；Second half-wave plate
HWP2 is used for the polarization state of the pumping pulse for adjusting incidence, and the pumping pulse after adjustment is incident to the 5th silver mirror M5；
Pumping pulse after polarization state adjustment is incident to the second lens L2 Jing after the 5th silver mirror M5 reflections；Second lens L2 is for entering
The pumping pulse penetrated is focused, and the pumping pulse after focusing is incident to orphan's generation module 24.
There is module 24 to protect mould field optical fiber LMA Fiber bigger than normal in orphan；Mould field optical fiber LMA Fiber bigger than normal are protected, is used for
Palarization multiplexing process is carried out to the pumping pulse after incident polarization state adjustment, is then produced according to the pumping pulse after process lonely
Sub- phenomenon of self-frequency shift, produces the high-energy orphan of two beam phase co-wavelengths and cross-polarization, and high-energy orphan described in two beams is entered
It is incident upon orphan's filter unit 3.
In Fig. 5, the guarantor mould field optical fiber LMA Fiber bigger than normal that core diameter is 40 microns are employed.There is provided in the present embodiment
Intensifier in, it is only necessary to before mould field optical fiber LMA Fiber bigger than normal are protected plus a half-wave plate HWP2, it is produced just
Hand over polarization solitons to propagate respectively along two main shafts for protecting mould field optical fiber LMA Fiber bigger than normal.With the sub- crystal bar of non-guarantor's polarisation
Compare, produced by mould field optical fiber bigger than normal is protected, after filtering after orphan can have more obvious modulated spectrum (a and b in Fig. 6),
And interfere auto-correlation mark (c and d in Fig. 6).In the present embodiment, two pumping pulses or orphan are there occurs in transmitting procedure
Cross-phase Modulation, and this follows different in non-situation about protecting in the sub- crystal bar of polarisation, because partially in the sub- crystal bar of non-guarantor's polarisation
The beam-dividing cube that shakes introduces optical delay, so two beam cross-polarization pumping pulses are kept completely separate in time.It is filtered
The energy of horizontal polarization and vertical polarization solitons is respectively 17.1 and receives burnt and 19.6 receive Jiao.After deconvolution is calculated, level is inclined
Measurement pulsewidth respectively 71 femtoseconds and 74 femtoseconds of orphan and the vertical polarization solitons of shaking.
Likewise, the present embodiment has used polarization multiplexing in mould field optical fiber bigger than normal is protected, carry out multi-photon imaging right
Than proving effect of the technology to signal enhancing.The present embodiment is contrasted to the two-photon fluorescence signal of mouse tail tendon, and
Applicability of the technology to different image modes is proved with this.Horizontal polarization SOLITON EXCITATION light (Fig. 7 a) and vertical polarization solitons swash
The signal value that luminous (Fig. 7 b) is produced is respectively in 22340 and 27070, Fig. 7, and colourity bar indicates signal level, and scale is 50
Micron.And excite the signal value for obtaining then to strengthen to 51600 simultaneously by two orphans.This shows that polarization multiplexing is same
The signal level for employing the multi-photon imaging signal for protecting mould field optical fiber bigger than normal can be strengthened.
In order to improve the signal level of multi-photon imaging signal, the invention provides above-described embodiment, wherein imaging signal
It is to be excited by the soliton produced due to soliton self-frequency sh in big mode field area fiber waveguide.With single polarization soliton
Excite and compare, polarization multiplexing actually doubles the repetition rate of orphan, and then proportionally improves signal.It is this
Technology can be applied in protecting inclined photonic crystal except applying in the sub- crystal bar of non-guarantor's polarisation and protect in mould field optical fiber bigger than normal
In rod.Three-photon fluorescent be imaged and two-photon fluorescence imaging both patterns phase of the multi-photon imaging results all with the present embodiment
Meet, i.e., palarization multiplexing can improve the signal level of multi-photon imaging signal.In the sub- crystal bar of non-guarantor's polarisation is applied, can
To increase more polarizing beam splitter cubes pair, the repetition rate of orphan proportional will be improved.Such as, a pair of polarizations point are increased
Beam cube, repetition rate will adds additional twice, and multi-photon imaging signal also increases twice, and total transmitance also can be compared with
Height, because each pair polarizing beam splitter cube merely add 6% loss on power.In addition, from the sub- crystal bar of non-guarantor's polarisation
The orphan of output is conllinear, is easy to follow-up multi-photon to be imaged.
Presently preferred embodiments of the present invention is the foregoing is only, not to limit the present invention, all essences in the present invention
Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.
Claims (10)
1. a kind of intensifier of multi-photon imaging signal, it is characterised in that including impulse generating unit, orphan's generation unit and
Orphan's filter unit：
The impulse generating unit, for producing the pumping pulse of preset repetition rate and pulsewidth, and the pumping pulse is entered
It is incident upon orphan's generation unit；
Orphan's generation unit, is carried out for the incident pumping pulse is adjusted to after the pumping pulse of preset polarization state
Palarization multiplexing process, and soliton self-frequency sh phenomenon is produced according to the pumping pulse after process obtain two beam phase co-wavelengths and orthogonal
The high-energy orphan of polarization, and high-energy orphan described in two beams is incident to into orphan's filter unit；
Orphan's filter unit, for being filtered process to two incident beam high-energy orphans, obtains the orphan of preset wavelength
Son, and the orphan after process is sent to into laser scanning microscope system as excitation signal, so that the scan laser microphotograph system
System carries out multi-photon imaging according to the excitation signal.
2. intensifier as claimed in claim 1, it is characterised in that the impulse generating unit includes that 1550 nano optical fibers swash
Light device and the first silver mirror；
The 1550 nano optical fibers laser instrument, for producing the pumping pulse of 1 MHz repetition and 500 femtosecond pulsewidths, and
The pumping pulse is incident to into first silver mirror；
First silver mirror reflects incident pumping pulse into orphan's generation unit.
3. intensifier as claimed in claim 1 or 2, it is characterised in that orphan's generation unit includes pulse adjustment mould
Block and orphan's generation module；
The pulse adjusting module, for the incident pumping pulse to be adjusted to the pumping pulse of preset polarization state and carry out
Then pumping pulse after process is incident to orphan's generation module by palarization multiplexing process；
Orphan's generation module, for producing soliton self-frequency sh phenomenon according to the pumping pulse after the incident process, obtains
To two beam phase co-wavelengths and cross-polarization high-energy orphan, and high-energy orphan described in two beams is incident to into orphan filter
Ripple unit.
4. intensifier as claimed in claim 3, it is characterised in that the pulse adjusting module include the first half-wave plate,
Two silver mirrors, the 3rd silver mirror, the 4th silver mirror, the first polarizing beam splitter cube, the second polarizing beam splitter cube and the first lens；
First half-wave plate is used for the polarization state of the pumping pulse for adjusting incidence, and will be the pumping pulse after adjustment incident
To second silver mirror；
Second silver mirror is reflected into first polarization beam splitting cube for the pumping pulse after incident polarization state is adjusted
Body；
Incident pumping pulse is divided into the first pumping subpulse and the second pumping subpulse by first polarizing beam splitter cube,
And the second pumping subpulse is incident to into second polarizing beam splitter cube；
The first pumping subpulse is after described in, the 3rd silver mirror and the 4th silver mirror carry out light path adjustment respectively, with second pump
Pu subpulse vertical incidence is to second polarizing beam splitter cube；
Second polarizing beam splitter cube for the first incident pumping subpulse and the second pumping subpulse are carried out conjunction beam,
Produce the pumping pulse of 1550 nanometers of cross-polarizations of conllinear transmission, and by 1550 nanometers of cross-polarizations of the conllinear transmission
Pumping pulse is incident to first lens；
First lens for being focused to the pumping pulse of 1550 nanometers of cross-polarizations of incident described conllinear transmission,
And the pumping pulse after focusing is incident to into orphan's generation module.
5. intensifier as claimed in claim 4, it is characterised in that it is 100 micro- that orphan's generation module is core diameter
The sub- crystal bar of non-guarantor's polarisation of rice.
6. intensifier as claimed in claim 1 or 2, it is characterised in that orphan's generation unit is also adjusted including light path
There is module in module and orphan；
The light path adjusting module, for the incident pumping pulse to be adjusted to the pumping pulse of preset polarization state, and will
Pumping pulse after polarization state adjustment is incident to the orphan and module occurs；
There is module in the orphan, for carrying out palarization multiplexing process to the pumping pulse after incident polarization state adjustment, then
Soliton self-frequency sh phenomenon is produced according to the pumping pulse after process, the high-energy for obtaining two beam phase co-wavelengths and cross-polarization is lonely
Son, and high-energy orphan described in two beams is incident to into orphan's filter unit.
7. intensifier as claimed in claim 6, it is characterised in that the light path adjusting module include the second half-wave plate,
Five silver mirrors and the second lens；
Second half-wave plate is used for polarization state of the adjustment by the incident pumping pulse of the impulse generating unit, and will adjust
Pumping pulse after whole is incident to the 5th silver mirror；
5th silver mirror is reflected into second lens for the pumping pulse after incident polarization state is adjusted；
Second lens are for being focused to incident pumping pulse, and the pumping pulse after focusing is incident to the orphan
There is module in son.
8. intensifier as claimed in claim 7, it is characterised in that the orphan occurs module to protect mould field optical fiber bigger than normal.
9. intensifier as claimed in claim 1, it is characterised in that orphan's filter unit includes the 3rd lens and optical filtering
Piece；
3rd lens, for two beam soliton pulses of incident diverging are collimated so that the two beams orphan is parallel
It is incident to the optical filter；
The optical filter, for being filtered process to the parallel orphan, obtains the orphan of preset wavelength, and by filtering
The orphan obtained after reason is sent to laser scanning microscope system as excitation signal.
10. intensifier as claimed in claim 9, it is characterised in that the optical filter is long wave pass filter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710013169.4A CN106532426B (en) | 2017-01-09 | 2017-01-09 | A kind of enhancement device of multi-photon imaging signal |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710013169.4A CN106532426B (en) | 2017-01-09 | 2017-01-09 | A kind of enhancement device of multi-photon imaging signal |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106532426A true CN106532426A (en) | 2017-03-22 |
| CN106532426B CN106532426B (en) | 2019-11-01 |
Family
ID=58335295
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710013169.4A Active CN106532426B (en) | 2017-01-09 | 2017-01-09 | A kind of enhancement device of multi-photon imaging signal |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106532426B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107144451A (en) * | 2017-05-10 | 2017-09-08 | 深圳大学 | The method for sealing multi-photon signal intensity in heavy water method and detection multi-photon imaging |
| CN108469412A (en) * | 2018-03-26 | 2018-08-31 | 深圳大学 | The self-reference measuring device of axial chromatic aberration in a kind of multiphoton microscope |
| CN109638616A (en) * | 2018-12-11 | 2019-04-16 | 杭州奕力科技有限公司 | Ultrashort pulse energy stability measuring device based on fibre-optical dispersion glistening light of waves orphan |
| CN109814282A (en) * | 2019-03-26 | 2019-05-28 | 深圳大学 | Rod-shaped photonic crystal fiber-based soliton synthesis method and device |
| CN110221445A (en) * | 2019-06-25 | 2019-09-10 | 深圳大学 | Circular polarization orphan generation device and multi-photon micro imaging system |
| WO2023050635A1 (en) * | 2021-09-29 | 2023-04-06 | 深圳大学 | Microscopic imaging apparatus |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0507508A2 (en) * | 1991-04-04 | 1992-10-07 | AT&T Corp. | Polarization multiplexing with solitons |
| US6339602B1 (en) * | 1999-01-26 | 2002-01-15 | Aisin Seiki Kabushiki Kaisha | Method of generating plural pulse lights and apparatus therefor |
| CN2585203Y (en) * | 2002-07-08 | 2003-11-05 | 中国科学院物理研究所 | Absolute self-calibrate device for quantum efficiency of single photon detector |
| CN102246448A (en) * | 2008-12-12 | 2011-11-16 | 阿尔卡特朗讯美国公司 | Optical communication using polarized transmit signal |
-
2017
- 2017-01-09 CN CN201710013169.4A patent/CN106532426B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0507508A2 (en) * | 1991-04-04 | 1992-10-07 | AT&T Corp. | Polarization multiplexing with solitons |
| US6339602B1 (en) * | 1999-01-26 | 2002-01-15 | Aisin Seiki Kabushiki Kaisha | Method of generating plural pulse lights and apparatus therefor |
| CN2585203Y (en) * | 2002-07-08 | 2003-11-05 | 中国科学院物理研究所 | Absolute self-calibrate device for quantum efficiency of single photon detector |
| CN102246448A (en) * | 2008-12-12 | 2011-11-16 | 阿尔卡特朗讯美国公司 | Optical communication using polarized transmit signal |
Non-Patent Citations (4)
| Title |
|---|
| CHRIS XU ET AL.: "Multiphoton Excitation of Molecular Fluorophores and Nonlinear Laser Microscopy", 《TOPICS IN FLUORESCENCE SPECTROSCOPY》 * |
| NORIHIKO NISHIZAWA ET AL.: "Simultaneous Generation of Wavelength Tunable Two-Colored Femtosecond Soliton Pulses Using Optical Fibers", 《IEEE PHOTONICS TECHNOLOGY LETTERS》 * |
| YUXIN WANG ET AL.: "Comparison of Signal Detection of GaAsP and GaAs PMTs for Multiphoton Microscopy at the 1700-nm window", 《IEEE PHOTONICS JOURNAL》 * |
| 陈永华: "多路分/合束光模块的工程实现及其应用研究", 《中国优秀硕士学位论文全文数据库 信息科技辑》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107144451A (en) * | 2017-05-10 | 2017-09-08 | 深圳大学 | The method for sealing multi-photon signal intensity in heavy water method and detection multi-photon imaging |
| CN107144451B (en) * | 2017-05-10 | 2020-02-11 | 深圳大学 | Heavy water sealing method and method for detecting multi-photon signal intensity in multi-photon imaging |
| CN108469412A (en) * | 2018-03-26 | 2018-08-31 | 深圳大学 | The self-reference measuring device of axial chromatic aberration in a kind of multiphoton microscope |
| CN109638616A (en) * | 2018-12-11 | 2019-04-16 | 杭州奕力科技有限公司 | Ultrashort pulse energy stability measuring device based on fibre-optical dispersion glistening light of waves orphan |
| CN109814282A (en) * | 2019-03-26 | 2019-05-28 | 深圳大学 | Rod-shaped photonic crystal fiber-based soliton synthesis method and device |
| CN109814282B (en) * | 2019-03-26 | 2022-05-13 | 深圳大学 | Rod-shaped photonic crystal fiber-based soliton synthesis method and device |
| CN110221445A (en) * | 2019-06-25 | 2019-09-10 | 深圳大学 | Circular polarization orphan generation device and multi-photon micro imaging system |
| WO2020258734A1 (en) * | 2019-06-25 | 2020-12-30 | 深圳大学 | Circularly polarized soliton generating device and multiphoton microscopic imaging system |
| WO2023050635A1 (en) * | 2021-09-29 | 2023-04-06 | 深圳大学 | Microscopic imaging apparatus |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106532426B (en) | 2019-11-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106532426B (en) | A kind of enhancement device of multi-photon imaging signal | |
| US11154189B2 (en) | Supercontinuum light source | |
| US10962751B2 (en) | Supercontinuum microscope for resonance and non-resonance enhanced linear and nonlinear images and time resolved microscope for tissues and materials | |
| EP2087400B1 (en) | Production of optical pulses at a desired wavelength using soliton self-frequency shift in higher-order-mode fiber | |
| US11128096B2 (en) | Femtosecond laser multimodality molecular imaging system | |
| Cadroas et al. | All-fiber femtosecond laser providing 9 nJ, 50 MHz pulses at 1650 nm for three-photon microscopy | |
| US7525724B2 (en) | Laser system for photonic excitation investigation | |
| DE112017001881B4 (en) | Supercontinuum source, method for generating and emitting a supercontinuum, multiphoton excitation fluorescence microscope and multiphoton excitation method | |
| CN105467572B (en) | Single wavelength realizes multi-photon pulses STED-SPIM microscopic systems | |
| Tong et al. | Manipulating soliton polarization in soliton self-frequency shift and its application to 3-photon microscopy in vivo | |
| JP2011257589A (en) | Laser microscope, laser microscope system and laser beam transmitting means | |
| Deguil et al. | Novel diode‐pumped infrared tunable laser system for multi‐photon microscopy | |
| CN112113940A (en) | Fluorescence imaging device and fluorescence imaging method | |
| WO2017175747A1 (en) | Supercontinuum-light-generating light source, supercontinuum-light generation method, multiphoton-excited fluorescence microscope, and multiphoton excitation method | |
| Saint-Jalm et al. | Fiber-based ultrashort pulse delivery for nonlinear imaging using high-energy solitons | |
| WO2023243052A1 (en) | Light source | |
| Tada et al. | Broadband coherent anti-Stokes Raman scattering spectroscopy using pulse-shaper-controlled variable-wavelength soliton pulses from a photonic crystal fiber | |
| Lialys | Near-Infrared Coherent Raman Spectroscopy and Microscopy | |
| Wang et al. | Coherent anti-Stokes Raman scattering imaging with photonic crytal fiber delivered laser source | |
| Harvey | An investigation of spectral broadening by nonlinear optical effects in photonic crystal fibers | |
| CN116345284A (en) | High-energy laser generation system, method and imaging system | |
| JP2022540869A (en) | All-fiber broadband tunable ultrafast laser source | |
| Rodríguez Suñé | Graphene based laser | |
| Yavaş | Ultrashort and short pulsed fiber laser development for transparent material processing, imaging and spectroscopy applications |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |