CN106526177A - Biomarker detection system and method based on colloidal gold test strip - Google Patents
Biomarker detection system and method based on colloidal gold test strip Download PDFInfo
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- CN106526177A CN106526177A CN201610982406.3A CN201610982406A CN106526177A CN 106526177 A CN106526177 A CN 106526177A CN 201610982406 A CN201610982406 A CN 201610982406A CN 106526177 A CN106526177 A CN 106526177A
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- gold strip
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
- G01N33/559—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody through a gel, e.g. Ouchterlony technique
Abstract
The invention discloses a biomarker detection system and method based on a colloidal gold test strip. The method comprises the following steps: scanning a two-dimensional code of the colloidal gold test strip by virtue of a camera to obtain two-dimensional code information of the colloidal gold test strip, and taking a detection result image of a biomarker from a detection band of the colloidal gold test strip; identifying a type of the biomarker according to the two-dimensional code information; locating a reaction color band position of the biomarker from the detection result image; segmenting a reaction color band region of the biomarker from the detection result image according to the reaction color band position; extracting a characteristic value of the reaction color band region, and calculating a gray value of the reaction color band region according to the extracted characteristic value; and comparing the gray value of the reaction color band region with the standard substance gray value to obtain a content detection result of the biomarker. The system and method which are disclosed by the invention have the advantages that the content of the biomarker on the colloidal gold test strip can be analyzed at any time and anywhere, and the deviation of the detection result caused by subjective factors or an error is avoided.
Description
Technical field
The present invention relates to the technical field of remote emergency, more particularly to a kind of biomarker based on colloidal gold strip
Detecting system and method.
Background technology
Colloidal gold immunochromatographitest test possesses plurality of advantages, such as quick diagnosis, reliable results, without the need for special installation and complexity
Operation skills etc., are widely used in many fields of biology, medical science, and chemicals, food and medicine inspection and quarantine etc.
Aspect.As colloidal gold strip testing result is mainly by estimating judgement, there are some defects in actual applications：(1) only
Qualitative detection can be carried out, it is impossible to realize quantitative measurement；(2) detectable substance concentration between marginal value when, show result be difficult with meat
Eye interpretation, the possibility that there is subjective erroneous judgement；(3) measurement process is it is possible that the situation of missing inspection or detection failure, such as false positive
And false negative；(4) traditional measurement result needs hand-kept, is unfavorable for that gold colloidal quick detection is promoted the use of, especially AIDS
The special detection article such as disease, drugs, should carry out result real time record according to the rules and retain evidence.
The content of the invention
To solve above-mentioned technical problem, present invention is primarily targeted at providing a kind of biology based on colloidal gold strip
Marker detection system and method, can whenever and wherever possible using the content detection for analyzing biomarker on colloidal gold strip
As a result, it is to avoid testing result deviation is caused due to subjective or error, and testing result is preserved, it is convenient to inquire about at any time.
For achieving the above object, the invention provides a kind of biomarker detecting system based on colloidal gold strip,
It is applied in cloud platform server, the cloud platform server sets up communication connection with mobile communication equipment by communication network,
The biomarker detecting system based on colloidal gold strip includes：
Image collection module, for the two dimension on the photographic head scanning colloidal gold strip by the mobile communication equipment
Code obtains the 2 D code information of colloidal gold strip, and the detection band intake by the photographic head from colloidal gold strip
The testing result image of biomarker；
Quick Response Code identification module, for identifying the biomarker according to the 2 D code information of colloidal gold strip
Type；
Image processing module, for the reaction color of biomarker is oriented from the testing result image of biomarker
Band position, and biomarker is partitioned into according to the reaction ribbon position of biomarker from the testing result image
Reaction color band region；
Quantitative analyses module, for extracting the eigenvalue of the reaction color band region, should according to the eigenvalue calculation extracted
The gray value of reaction color band region, the gray value of reaction color band region is compared with standard substance gray value, and according to pre-
The relatively regular compared result of definite proportion carries out the content detection result that numerical quantization obtains biomarker.
Preferably, described image acquisition module is additionally operable to the pollution impure point in the testing result image by biomarker
It is purged process.
Preferably, the described biomarker detecting system based on colloidal gold strip also includes data storage module,
The data storage module is for the storage list that is stored in the content detection result of the biomarker in cloud platform server
In unit.
Preferably, the colloidal gold strip includes Quick Response Code and detection band, and the Quick Response Code is solidified with Quick Response Code letter
Breath, the 2 D code information include the type of biomarker, date of manufacture, effect duration information, are provided with institute's detection band
The nitrocellulose membrane of white, for detecting the content concn of biomarker.
Preferably, the predetermined comparison rule is defined as follows：If the color of the reaction color band region of the biomarker
For dark color, then the content concn of the biomarker is high；If the color of the reaction color band region of the biomarker is light color
Or color does not change, then the content concn of the biomarker is low.
Present invention also offers a kind of biological marker object detecting method based on colloidal gold strip, is applied to cloud platform clothes
In business device, the cloud platform server sets up communication connection by communication network and mobile communication equipment, described based on gold colloidal
The biological marker object detecting method of test strips includes step：
The Quick Response Code scanned by the photographic head of the mobile communication equipment on colloidal gold strip obtains colloid gold test paper
The 2 D code information of bar, and the detection knot of the detection band intake biomarker by the photographic head from colloidal gold strip
Fruit image；
The type of the biomarker is identified according to the 2 D code information of colloidal gold strip；
The reaction ribbon position of biomarker is oriented from the testing result image of biomarker；
The anti-of biomarker is partitioned into according to the reaction ribbon position of biomarker from the testing result image
Answer color band region；
The eigenvalue of the reaction color band region is extracted, and according to the ash of the eigenvalue calculation reaction color band region for extracting
Angle value；
The gray value of reaction color band region is compared with standard substance gray value, and according to predetermined comparison rule to comparing
As a result carry out the content detection result that numerical quantization obtains biomarker.
Preferably, the biological marker object detecting method based on colloidal gold strip also includes step：By biological marker
Pollution impure point in the testing result image of thing is purged process.
Preferably, the biological marker object detecting method based on colloidal gold strip also includes step：By the biology
The content detection result of mark is stored in the memory element in cloud platform server.
Preferably, the colloidal gold strip includes Quick Response Code and detection band, and the Quick Response Code is solidified with Quick Response Code letter
Breath, the 2 D code information include the type of biomarker, date of manufacture, effect duration information, are provided with institute's detection band
The nitrocellulose membrane of white, for detecting the content concn of biomarker.
Preferably, the predetermined comparison rule is defined as follows：If the color of the reaction color band region of the biomarker
For dark color, then the content concn of the biomarker is high；If the biomarker reaction color band region color for light color or
Color does not change, then the content concn of the biomarker is low.
Compared to prior art, the biomarker detecting system and method based on colloidal gold strip of the present invention is led to
The testing result image crossed on intake colloidal gold colloidal gold detection test paper strip, you can the content results of biomarker are quantitatively examined
Survey, the interpretation skill or training that the detection colour band on colloidal gold colloidal gold detection test paper strip is read without professional can provide objective knot
Really, it is to avoid personal error, prevent missing inspection；Testing result is directly stored in health control cloud platform, facilitates user's real-time query；
Using mobile communication terminal (such as smart mobile phone) and wireless network, facilitate portable, be capable of achieving to be detected whenever and wherever possible.This
Outward, Quick Response Code is added on colloidal gold colloidal gold detection test paper strip, facilitate system to be identified matching, therefore using different types of glue
Body gold test paper strip is detecting the content concn of different biomarkers.
Description of the drawings
Fig. 1 is that the present invention is shown based on the application architecture of the biomarker detecting system preferred embodiment of colloidal gold strip
It is intended to；
Fig. 2 is flow chart of the present invention based on the biological marker object detecting method preferred embodiment of colloidal gold strip.
The realization of the object of the invention, functional characteristics and advantage will be described further in conjunction with the embodiments referring to the drawings.
Specific embodiment
Further to illustrate the present invention to reach technological means and effect that above-mentioned purpose is taken, below in conjunction with accompanying drawing
And preferred embodiment, specific embodiment of the invention, structure, feature and its effect are described in detail.It should be appreciated that this
The described specific embodiment in place is not intended to limit the present invention only to explain the present invention.
With reference to shown in Fig. 1, Fig. 1 is biomarker detecting system preferred embodiment of the present invention based on colloidal gold strip
Application architecture schematic diagram.In the present embodiment, the biomarker detecting system 10 based on colloidal gold strip is run
In cloud platform server 1, the cloud platform server 1 also includes communication unit 11, memory element 12 and processing unit 13.Institute
State cloud platform server 1 and communication connection is set up with mobile communication equipment 2 by communication network 3.The cloud platform server 1 is one
A server in cloud platform is planted, by the data transmission capabilities and data-handling capacity of cloud platform server 1, can be more preferable
Ground processes the testing result image with transmission biomarker.
The mobile communication equipment 2 includes photographic head 20, and the photographic head 20 is used for scanning and had detected biomarker
Quick Response Code (QR Code) 41 and detection band (Test area) 42 on colloidal gold strip 4 absorbs the detection of biomarker
Result images.The mobile communication equipment 2 sends the testing result image of the biomarker of colloidal gold strip 4 to cloud and puts down
Platform server 1.In the present embodiment, the mobile communication equipment 2 can be the smart mobile phone with photographing module, flat board electricity
Brain, personal digital assistant (Personal Digital Assistant, PDA), PC, mobile communication terminal etc. other appoint
Anticipate suitable portable electronic address equipment.The communication network 3 can be a kind of wireline communication network or cordless communication network.
In the present embodiment, the communication network 3 be preferably cordless communication network, including but not limited to, GSM network, GPRS network,
The wireless-transmission networks such as cdma network, TD-SCDMA network, WiMAX network, TD-LTE networks, FDD-LTE networks.
The colloidal gold strip 4 includes Quick Response Code (QR Code) 41 and detection band (Test area) 42, described two
Dimension code 41 is solidified with 2 D code information, and the 2 D code information includes type (the i.e. colloidal gold strip for detecting biomarker
For detecting the type of biomarker, such as HIV (human immunodeficiency virus), hepatitis B virus), the date of manufacture, the information such as effect duration.The inspection
The quantity of measuring tape 42 can be one, or multiple, different detection bands 42 is used to detect different biomarkers
(Biomarker) a kind of, i.e., biomarker of one detection band, 42 detections, the biomarker can be HIV (human immunodeficiency virus)
HIV antigens, HBV antigens of hepatitis B virus etc..White or colourless nitrocellulose membrane is provided with the detection band 42, for examining
Survey the content concn of the biomarker.
The communication unit 11 is a kind of wireless communication interface with long-distance radio communication function or near-field communication function,
For example, support that the communication of the mechanicss of communication such as GSM, GPRS, WCDMA, CDMA, TD-SCDMA, WiMAX, TD-LTE, FDD-LTE connects
Mouthful, the testing result image of biomarker of the communication unit 11 for sending from mobile communication equipment 2.The storage is single
Unit 12 can be a kind of read-only memory unit ROM, electrically-erasable memory element EEPROM, flash memory cell FLASH or solid
Hard disk etc..The processing unit 13 can be a kind of central processing unit (Central Processing Unit, CPU), microcontroller
Device (MCU), data processing chip or the information process unit with data processing function.
In the present embodiment, the biomarker detecting system 10 based on colloidal gold strip includes, but do not limit to
In image collection module 101, Quick Response Code identification module 102, image processing module 103, quantitative analyses module 104 and data
Preserving module 105.Module alleged by the present invention is referred to and a kind of can be performed simultaneously by the processing unit 13 of the cloud platform server 1
And the series of computation machine programmed instruction section of fixing function can be completed, which is stored in the storage list of the cloud platform server 1
In unit 12.
Described image acquisition module 101 is used to scan colloidal gold strip 40 by the photographic head 20 of mobile communication equipment 2
On Quick Response Code 41 obtain the 2 D code information of colloidal gold strip 40, and the detection band 42 from colloidal gold strip 40 takes the photograph
Take the testing result image of biomarker.In the present embodiment, the mobile communication equipment 2 scans colloid by photographic head 20
Quick Response Code 41 on gold test paper strip 4 obtains the 2 D code information of colloidal gold strip 40 and the inspection from colloidal gold strip 40
Measuring tape 42 absorbs the testing result image of biomarker, and believes the Quick Response Code of colloidal gold strip 40 by communication network 3
The testing result image of breath and biomarker is sent to cloud platform server 1.
The Quick Response Code identification module 102 is for identifying biological marker according to the 2 D code information of colloidal gold strip 4
The type of thing.As each colloidal gold strip 4 includes the Quick Response Code 41 for being solidified with 2 D code information, the 2 D code information
The type of detection biomarker, therefore Quick Response Code identification module 102 are included by the Quick Response Code of identification colloidal gold strip 4
Information is achieved with the type of biomarker, for example, the type such as the HIV antigens of HIV (human immunodeficiency virus), HBV antigens of hepatitis B virus.
Described image processing module 103 is carried out clearly for the pollution impure point in the testing result image by biomarker
Except process.Specifically, image processing module 103 is clicked through to the pollution impurity in testing result image using a kind of median filtering method
Row removing is processed, and makes testing result image be close to original target image as far as possible, so as to obtain clean testing result image.In described
Value filtering is based on a kind of theoretical nonlinear properties image processing techniquess that can effectively suppress noise of sequencing statistical, medium filtering
Ultimate principle be each point pixel value in a neighborhood the pixel value of any in digital picture or Serial No. with the point
Mesophyticum is replaced, and allows the pixel value of surrounding to be close to actual value, makes image be close to original as far as possible so as to eliminate the noise spot isolated in image
Target image.
Described image processing module 103 is additionally operable to from the testing result image reaction color for orienting biomarker
Band position, and the reaction of biomarker is partitioned into according to the reaction ribbon position of biomarker from testing result image
Color band region.In the present embodiment, image processing module 103 orients the inspection of colloidal gold strip 4 in testing result image
Measuring tape image can orient the reaction ribbon position of biomarker.Testing result image is divided into by image processing module 103
The reaction colour band of biomarker and unreacted band is made to be separated for multiple regions, due to the detection band 42 of colloidal gold strip 4
The nitrocellulose membrane of detection biomarker is provided with, the nitrocellulose membrane is not coated with reaction white is presented during biomarker
, there is dark red colour response colour band when scribbling biomarker and reacting.The present embodiment carries out reaction color to testing result image
Zone regional partition, purpose are exactly that responseless white background is separated with the developed band for color change occur, are conducive to
Subsequent extracted reacts the eigenvalue of colour band.
The quantitative analyses module 104 is used for the eigenvalue for extracting reaction color band region, and according to the eigenvalue meter for extracting
Calculate the gray value of the reaction color band region.In the present embodiment, the eigenvalue of the reaction color band region refers to reaction colour band area
The pixel value (pixel) in domain, quantitative analyses module 10 is according to the calculated for pixel values reaction color band region of reaction color band region
Gray value.The quantitative analyses module 104 is additionally operable to the gray value of reaction color band region is compared with standard substance gray value,
And the content detection result that numerical quantization obtains biomarker is carried out according to predetermined comparison rule compared result.In this reality
Apply in example, when the standard substance gray value refers to the biomarker in detection biology or medical domain, do not produce pathological changes
Biomarker and colloidal gold strip 40 on produce the image intensity value of reaction colour band, which define image intensity value and life
The numerical relation of thing marker concentration.The predetermined comparison rule is defined as follows：If the reaction colour band area of the biomarker
The color in domain is dark (being for example changed into peony from white), then the content concn of the biomarker is high；If the biology
The color of the reaction color band region of mark is that light (being for example changed into light red from white) or color do not change, then described
The content concn of biomarker is low.Quantitative analyses module 10 can be to biology according to the predetermined comparison rule and comparative result
The content of mark carries out numerical quantization (quantifying for example with value class) and the concentration of biomarker is obtained.
The data storage module 105 is for being stored in cloud platform server 1 by the content detection result of biomarker
In memory element 12 in carry out inquiry reference in the future for doctor, be diagnosis certain disease (such as hepatitis B virus) conduct
Auxiliary foundation.In the present embodiment, the content detection result of biomarker is stored in health by the data storage module 105
In management cloud platform server, convenient inquiry reference in the future, meanwhile, user can also log in health control cloud platform real-time query
Testing result.
To realize the object of the invention, present invention also offers a kind of biological marker analyte detection side based on colloidal gold strip
Method, is applied in cloud platform server 1 as shown in Figure 1.As shown in Fig. 2 Fig. 2 is life of the present invention based on colloidal gold strip
The flow chart of thing marker detection method preferred embodiment.In the present embodiment, the biology mark based on colloidal gold strip
Will object detecting method comprises the steps S21 to step S28：
Step S21, the Quick Response Code for scanning colloidal gold strip by the photographic head of mobile communication equipment obtain Quick Response Code letter
Breath, absorbs the testing result image of biomarker from the detection zone of colloidal gold strip；Specifically, image collection module 101
The Quick Response Code 41 scanned by the photographic head 20 of mobile communication equipment 2 on colloidal gold strip 40 obtains colloidal gold strip 40
2 D code information, and the testing result image of the intake biomarker of detection band 42 from colloidal gold strip 40.
Step S22, identifies the type of biomarker according to the 2 D code information of colloidal gold strip；Specifically, two
Dimension code identification module 102 identifies the type of biomarker according to the 2 D code information of colloidal gold strip 4.Due to each
Colloidal gold strip 4 includes the Quick Response Code 41 for being solidified with 2 D code information, and the 2 D code information includes detection biological marker
The type of thing, therefore Quick Response Code identification module 102 is achieved with biological mark by recognizing the 2 D code information of colloidal gold strip 4
The type of will thing, for example, the type such as the HIV antigens of HIV (human immunodeficiency virus), HBV antigens of hepatitis B virus.
Pollution impure point in the testing result image of biomarker is purged process by step S23；Specifically,
Image processing module 103 is purged process to the pollution impure point in testing result image using a kind of median filtering method, makes
Testing result image is close to original target image as far as possible, so as to obtain clean testing result image.The medium filtering is to be based on
A kind of theoretical nonlinear properties image processing techniquess that can effectively suppress noise of sequencing statistical, the ultimate principle of medium filtering is
In a neighborhood the pixel value of any in digital picture or Serial No. with the point, the Mesophyticum of each point pixel value is replaced, and allows week
The pixel value for enclosing is close to actual value, makes image be close to original target image as far as possible so as to eliminate the noise spot isolated in image.
Step S24, orients the reaction ribbon position of biomarker from testing result image；Specifically, at image
Reason module 103 orients the reaction ribbon position of biomarker from the testing result image.In the present embodiment, image
Processing module 103 orients biological marker by the detection band image of colloidal gold strip 4 is identified in testing result image
The reaction ribbon position of thing.
Step S25, is partitioned into biomarker according to the reaction ribbon position of biomarker from testing result image
Reaction color band region；Specifically, image processing module 103 according to the reaction ribbon position of biomarker from testing result figure
The reaction color band region of biomarker is partitioned into as in.In the present embodiment, image processing module 103 is by testing result image
Being divided into multiple regions makes the reaction colour band of biomarker and unreacted band be separated, due to the detection of colloidal gold strip 4
With 42 nitrocellulose membranes for being provided with detection biomarker, the nitrocellulose membrane is not coated with reaction is presented in vain during biomarker
Color, there is dark red colour response colour band when scribbling biomarker and reacting.The present embodiment is reacted to testing result image
Color band region is split, and purpose is exactly that responseless white background is separated with the developed band for color change occur, favorably
The eigenvalue of colour band is reacted in subsequent extracted.
Step S26, extracts the eigenvalue of reaction color band region, and according to the eigenvalue calculation reaction color band region for extracting
Gray value；Specifically, quantitative analyses module 104 extracts the eigenvalue of reaction color band region, and according to the eigenvalue meter for extracting
Calculate the gray value of the reaction color band region.In the present embodiment, the eigenvalue of the reaction color band region refers to reaction colour band area
The pixel value (pixel) in domain, quantitative analyses module 10 is according to the calculated for pixel values reaction color band region of reaction color band region
Gray value.
Step S27, the gray value of reaction color band region is compared with standard substance gray value, and compares rule according to predetermined
Then compared result carries out the content detection result that numerical quantization obtains biomarker；Specifically, quantitative analyses module 104 will
The gray value of reaction color band region is compared with standard substance gray value, and enters line number according to predetermined comparison rule compared result
Value quantifies the content detection result for obtaining biomarker.In the present embodiment, the standard substance gray value is referred in detection life
During biomarker in thing or medical domain, do not produce produce on the biomarker of pathological changes and colloidal gold strip 40 it is anti-
The image intensity value of colour band is answered, the numerical relation of image intensity value and biomarker concentration is which defined.It is described to make a reservation for compare
Rule is as follows：If the color of the reaction color band region of the biomarker is dark (such as being changed into peony from white),
The content concn of the biomarker is high；If the biomarker reaction color band region color for light color (for example by
White is changed into light red) or color do not change, then the content concn of the biomarker is low.Quantitative analyses module 10
Numerical quantization can be carried out (for example with numerical value etc. to the content of biomarker according to the predetermined comparison rule and comparative result
Level quantifies) concentration of biomarker is obtained.
Step S28, the content detection result of biomarker is stored in cloud platform server；Specifically, data are protected
For doctor in the memory element 12 that storing module 105 is stored in the content detection knot of biomarker in cloud platform server 1
Inquiry reference is carried out in the future, is certain disease of diagnosis (such as hepatitis B virus) conduct auxiliary foundation.In the present embodiment, institute
State data storage module 105 the content detection result of biomarker is stored in health control cloud platform server, it is convenient
Inquiry reference in the future, meanwhile, user can also log in health control cloud platform real-time query testing result.
Biomarker detecting system and method based on colloidal gold strip of the present invention is by absorbing gold colloidal inspection
Testing result image on test paper slip, you can detection by quantitative is carried out to the content results of biomarker, without professional
The interpretation skill or training for reading the detection colour band on colloidal gold colloidal gold detection test paper strip can provide objective results, it is to avoid artificially by mistake
Difference, prevents missing inspection；Testing result is directly stored in health control cloud platform, facilitates user's real-time query；Using mobile communication
Terminal (such as smart mobile phone) and wireless network, facilitate portable, are capable of achieving to be detected whenever and wherever possible.Additionally, in gold colloidal
Quick Response Code is added in test strip, facilitates system to be identified matching, therefore using different types of colloidal gold strip
To detect the content concn of different biomarkers.
The preferred embodiments of the present invention are these are only, the scope of the claims of the present invention is not thereby limited, it is every using this
Equivalent structure or equivalent function conversion that bright description and accompanying drawing content are made, or directly or indirectly it is used in other related skills
Art field, is included within the scope of the present invention.
Claims (10)
1. a kind of biomarker detecting system based on colloidal gold strip, is applied in cloud platform server, and the cloud is put down
Platform server sets up communication connection with mobile communication equipment by communication network, it is characterised in that described based on colloid gold test paper
The biomarker detecting system of bar includes：
Image collection module, obtains for the Quick Response Code on the photographic head scanning colloidal gold strip by the mobile communication equipment
Obtain the 2 D code information of colloidal gold strip, and the detection band intake biology by the photographic head from colloidal gold strip
The testing result image of mark；
Quick Response Code identification module, for the class of the biomarker is identified according to the 2 D code information of colloidal gold strip
Type；
Image processing module, for the reaction colour band position of biomarker is oriented from the testing result image of biomarker
Put, and be partitioned into the reaction of biomarker according to the reaction ribbon position of biomarker from the testing result image
Color band region；
Quantitative analyses module, for extracting the eigenvalue of the reaction color band region, according to the eigenvalue calculation reaction extracted
The gray value of color band region, the gray value of reaction color band region is compared with standard substance gray value, and according to pre- definite proportion
Relatively regular compared result carries out the content detection result that numerical quantization obtains biomarker.
2. the biomarker detecting system based on colloidal gold strip as claimed in claim 1, it is characterised in that the figure
The pollution impure point being additionally operable to as processing module in the testing result image by biomarker is purged process.
3. the biomarker detecting system based on colloidal gold strip as claimed in claim 1, it is characterised in that the system
Also include data storage module, the data storage module is put down for the content detection result of the biomarker is stored in cloud
In memory element in platform server.
4. the biomarker detecting system based on colloidal gold strip as claimed in claim 1, it is characterised in that the glue
Body gold test paper strip includes Quick Response Code and detection band, and the Quick Response Code is solidified with 2 D code information, and the 2 D code information includes inspection
The type of survey biomarker, date of manufacture, effect duration information, are provided with the nitrocellulose membrane of white in the detection band, use
In the content concn of detection biomarker.
5. the biomarker detecting system based on colloidal gold strip as claimed in claim 4, it is characterised in that described pre-
Definite proportion is relatively regular to be defined as follows：
If the color of the reaction color band region of the biomarker is dark color, the content concn of the biomarker is high；
If the color of the reaction color band region of the biomarker is that light color or color do not change, the biological marker
The content concn of thing is low.
6. a kind of biological marker object detecting method based on colloidal gold strip, is applied in cloud platform server, and the cloud is put down
Platform server sets up communication connection with mobile communication equipment by communication network, it is characterised in that described based on colloid gold test paper
The biological marker object detecting method of bar includes step：
The Quick Response Code scanned by the photographic head of the mobile communication equipment on colloidal gold strip obtains colloidal gold strip
2 D code information, and the detection band by the photographic head from colloidal gold strip absorbs the testing result figure of biomarker
Picture；
The type of the biomarker is identified according to the 2 D code information of colloidal gold strip；
The reaction ribbon position of biomarker is oriented from the testing result image of biomarker；
The reaction color of biomarker is partitioned into from the testing result image according to the reaction ribbon position of biomarker
Region；
The eigenvalue of the reaction color band region is extracted, and according to the gray scale of the eigenvalue calculation reaction color band region for extracting
Value；
The gray value of reaction color band region is compared with standard substance gray value, and according to predetermined comparison rule compared result
Carry out the content detection result that numerical quantization obtains biomarker.
7. the biological marker object detecting method based on colloidal gold strip as claimed in claim 6, it is characterised in that the method
Also include step：Pollution impure point in the testing result image of biomarker is purged into process.
8. the biological marker object detecting method based on colloidal gold strip as claimed in claim 6, it is characterised in that the method
Also include step：The content detection result of the biomarker is stored in the memory element in cloud platform server.
9. the biological marker object detecting method based on colloidal gold strip as claimed in claim 6, it is characterised in that the glue
Body gold test paper strip includes Quick Response Code and detection band, and the Quick Response Code is solidified with 2 D code information, and the 2 D code information includes inspection
The type of survey biomarker, date of manufacture, effect duration information, are provided with the nitrocellulose membrane of white in the detection band, use
In the content concn of detection biomarker.
10. the biological marker object detecting method based on colloidal gold strip as claimed in claim 9, it is characterised in that described
Predetermined comparison rule is defined as follows：
If the color of the reaction color band region of the biomarker is dark color, the content concn of the biomarker is high；
If the color of the reaction color band region of the biomarker is that light color or color do not change, the biological marker
The content concn of thing is low.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610982406.3A CN106526177A (en) | 2016-11-05 | 2016-11-05 | Biomarker detection system and method based on colloidal gold test strip |
| PCT/CN2017/080703 WO2018082257A1 (en) | 2016-11-05 | 2017-04-17 | Biomarker detecting system and method based on colloidal gold test strip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610982406.3A CN106526177A (en) | 2016-11-05 | 2016-11-05 | Biomarker detection system and method based on colloidal gold test strip |
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| Publication Number | Publication Date |
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| CN106526177A true CN106526177A (en) | 2017-03-22 |
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| CN201610982406.3A Pending CN106526177A (en) | 2016-11-05 | 2016-11-05 | Biomarker detection system and method based on colloidal gold test strip |
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| CN107356737A (en) * | 2017-07-03 | 2017-11-17 | 华南理工大学 | A kind of preparation method of Activity determination Quick Response Code |
| CN107449907A (en) * | 2017-07-28 | 2017-12-08 | 华中科技大学 | The lateral flow chromatography detection card and detection method of a kind of encryption |
| CN107561072A (en) * | 2017-09-14 | 2018-01-09 | 深圳泰首智能技术有限公司 | The accurate interpretation method and its interpretation device for realizing PUC T test results |
| WO2018082257A1 (en) * | 2016-11-05 | 2018-05-11 | 深圳市前海安测信息技术有限公司 | Biomarker detecting system and method based on colloidal gold test strip |
| CN110133261A (en) * | 2019-06-12 | 2019-08-16 | 湖南伊鸿健康科技有限公司 | A kind of detection device of colloid gold test paper colour developing result, system and method |
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| US10991185B1 (en) | 2020-07-20 | 2021-04-27 | Abbott Laboratories | Digital pass verification systems and methods |
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| WO2013120191A1 (en) * | 2012-02-17 | 2013-08-22 | Vitrum Diagnostics Inc. | Informative diagnostic kit and device for immunochromatographic detection |
| CN103248681B (en) * | 2013-04-27 | 2016-04-27 | 北京福乐云检测科技有限公司 | Based on the colloidal gold method detection cloud platform that mobile terminal reads |
| CN104964973A (en) * | 2015-07-08 | 2015-10-07 | 邓双胜 | Test paper reading and analyzing method and system based on mobile terminal camera |
| CN105137058A (en) * | 2015-08-13 | 2015-12-09 | 张树华 | Immunoassay and biochemical detection system |
| CN106526177A (en) * | 2016-11-05 | 2017-03-22 | 深圳市前海安测信息技术有限公司 | Biomarker detection system and method based on colloidal gold test strip |
-
2016
- 2016-11-05 CN CN201610982406.3A patent/CN106526177A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2018082257A1 (en) * | 2016-11-05 | 2018-05-11 | 深圳市前海安测信息技术有限公司 | Biomarker detecting system and method based on colloidal gold test strip |
| CN107356737A (en) * | 2017-07-03 | 2017-11-17 | 华南理工大学 | A kind of preparation method of Activity determination Quick Response Code |
| CN107449907A (en) * | 2017-07-28 | 2017-12-08 | 华中科技大学 | The lateral flow chromatography detection card and detection method of a kind of encryption |
| CN107449907B (en) * | 2017-07-28 | 2019-03-05 | 华中科技大学 | A kind of the lateral flow chromatography detection card and detection method of encryption |
| CN107561072A (en) * | 2017-09-14 | 2018-01-09 | 深圳泰首智能技术有限公司 | The accurate interpretation method and its interpretation device for realizing PUC T test results |
| CN107561072B (en) * | 2017-09-14 | 2020-02-11 | 深圳泰首智能技术有限公司 | Interpretation method and interpretation device for accurately realizing PUC-T test result |
| CN110133261A (en) * | 2019-06-12 | 2019-08-16 | 湖南伊鸿健康科技有限公司 | A kind of detection device of colloid gold test paper colour developing result, system and method |
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| WO2018082257A1 (en) | 2018-05-11 |
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