CN106525272B - Thermo-sensitive biosensor - Google Patents
Thermo-sensitive biosensor Download PDFInfo
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- CN106525272B CN106525272B CN201610891995.4A CN201610891995A CN106525272B CN 106525272 B CN106525272 B CN 106525272B CN 201610891995 A CN201610891995 A CN 201610891995A CN 106525272 B CN106525272 B CN 106525272B
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- liquid crystal
- dna
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- molecule
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01K—MEASURING TEMPERATURE; MEASURING QUANTITY OF HEAT; THERMALLY-SENSITIVE ELEMENTS NOT OTHERWISE PROVIDED FOR
- G01K11/00—Measuring temperature based upon physical or chemical changes not covered by groups G01K3/00, G01K5/00, G01K7/00 or G01K9/00
Abstract
The invention discloses Thermo-sensitive biosensors.The Thermo-sensitive biosensor includes：Liquid crystal molecule；Parent dna molecule, the parent dna molecule have hydrophobic side and water-wet side, and the hydrophobic side is connected with the liquid crystal molecule；And connection DNA, the both ends of the connection DNA are connected with the water-wet side of the parent dna molecule respectively.The Thermo-sensitive biosensor can sensitively reflect the variation of environment temperature by the polymerization and depolymerization that connect DNA, to by the state for observing liquid crystal droplet, understand the variation of temperature.
Description
Technical field
The present invention relates to bioengineering fields, and in particular, to Thermo-sensitive biosensor.
Background technology
Liquid crystal state is a kind of between solid crystals and unordered liquid " soft polymorphic material ".Liquid crystal molecule has with long-range
Sequence and optical anisotropy have been widely used in plane display field.Univ Wisconsin-Madison USA Abbott is ground within 1998
Study carefully group utilizes liquid crystal molecule to build biosensor for the first time, opens liquid crystal applications frontier.Liquid crystal biosensor is easy to real
Now micromation and array have the advantages that simple in structure, detection is easy to be quick, pacify in life science, clinical medicine and food
Full field presents wide application prospect.Liquid crystal droplet temperature sensor at present, there are the change in orientation of liquid crystal droplet is unknown
It is aobvious, the problem of temperature sensitivity difference.
The Thermo-sensitive biosensor that liquid crystal droplet is constituted as a result, has much room for improvement.
Invention content
The present invention is directed at least solve one of the technical problems existing in the prior art.For this purpose, one object of the present invention
It is to propose that a kind of Thermo-sensitive biosensor, the sensor connect the change of the polymerization and depolymerization reflection temperature of DNA by control
Change, when temperature increases to over predetermined temperature, that is, connect DNA fusing points, connection DNA will appear fracture so as to cause liquid crystal droplet from group
Poly- state becomes dispersity, and when temperature is less than predetermined temperature, liquid crystal droplet can be changed into aggregating state from dispersity again,
To by the state for observing liquid crystal droplet, understand the variation of temperature.
Thus, according to an aspect of the present invention, the present invention provides a kind of Thermo-sensitive biosensors.According to the present invention
Embodiment, which includes：Liquid crystal molecule；Parent dna molecule, the parent dna molecule have hydrophobic
End and water-wet side, the hydrophobic side are connected with the liquid crystal molecule；And connection DNA, it is described connection DNA both ends respectively with institute
The water-wet side for stating parent dna molecule is connected.
Thermo-sensitive biosensor according to the ... of the embodiment of the present invention, connection DNA are high to the susceptibility of its fusing point, pass through connection
The polymerization and depolymerization of DNA can sensitively reflect the variation of environment temperature, and in turn, inventor is interacted real by Van der Waals force
The hydrophobic side of parent dna molecule and the combination of liquid crystal molecule are showed, have recycled connection DNA connection parent dna molecules, pass through control
The variation for connecting the polymerization and depolymerization reflection environment temperature of DNA connects DNA fusing points, even when temperature increases to over predetermined temperature
Meeting DNA and will appear fracture so as to cause liquid crystal droplet becomes dispersity from aggregating state, when temperature is less than predetermined temperature, liquid
Brilliant drop can be changed into aggregating state from dispersity again, to by the state for observing liquid crystal droplet, understand the variation of temperature.
The sensitivity and accuracy of the Thermo-sensitive biosensor are high as a result,.Used in the present invention simultaneously is the liquid for having liquid crystalline phase
Brilliant molecule, since liquid crystal has birefringent phenomenon so that polarisation can be used to carry out sensing outcome for the Thermo-sensitive biosensor
Observation has the advantage for facilitating observation.
In addition, Thermo-sensitive biosensor according to the above embodiment of the present invention, can also have following additional technology
Feature：
According to an embodiment of the invention, the liquid crystal molecule has liquid crystalline phase at room temperature.The liquid crystal droplet can be with as a result,
The detection that can carry out the change in orientation of liquid crystal droplet at ambient temperature, ensures that the property states of liquid crystal droplet are intact.
According to an embodiment of the invention, the liquid crystal molecule is selected from 5CB liquid crystal molecules, TL205 liquid crystal molecules and E7 liquid
At least one of brilliant molecule.Liquid crystal molecule has liquid crystalline phase at room temperature as a result, and liquid crystal droplet quantity is more, homogeneity is good.
According to an embodiment of the invention, the hydrophobic side is fat chain.It is easily connected to liquid crystal droplet as a result,.
According to an embodiment of the invention, the hydrophobic side is alkyl chain.It is easily connected to liquid crystal droplet as a result,.
According to an embodiment of the invention, the water-wet side is the single strand dna with same end.As a result, be convenient for
DNA is connected to combine.
According to an embodiment of the invention, the both ends of the connection DNA and the water-wet side are complementary.Be conducive to connect as a result,
DNA is combined with parent dna complementary element.
According to an embodiment of the invention, the Thermo-sensitive biosensor is controlled by the complementary series of the connection DNA
Respond temperature.The complementary series of connection DNA is by influencing fusing point control response temperature as a result,.
According to an embodiment of the invention, the response temperature of the Thermo-sensitive biosensor is 20-85 DEG C.It is temperature sensitive as a result,
Property biosensor sensitivity and accuracy it is high.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
Obviously, or practice through the invention is recognized.
Description of the drawings
The above-mentioned and/or additional aspect and advantage of the present invention will become in the description from combination following accompanying drawings to embodiment
Obviously and it is readily appreciated that, wherein：
Fig. 1 shows the structural schematic diagram of Thermo-sensitive biosensor according to an embodiment of the invention；
Fig. 2 shows the optical change schematic diagram of liquid crystal droplet；
Fig. 3 shows the schematic diagram of depolymerization and the polymerization of Thermo-sensitive biosensor according to an embodiment of the invention；
Fig. 4 shows the microstructure schematic diagram of liquid crystal droplet according to an embodiment of the invention；
Fig. 5 shows the micro-image of depolymerization and the polymerization of Thermo-sensitive biosensor according to an embodiment of the invention
Schematic diagram；
Fig. 6 shows the fusing point T of connection DNA according to an embodiment of the inventionmThe schematic diagram of curve；
Fig. 7 shows the picture schematic diagram of each stage Thermo-sensitive biosensor according to an embodiment of the invention.
Specific implementation mode
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning to end
Same or similar label indicates same or similar element or element with the same or similar functions.Below with reference to attached
The embodiment of figure description is exemplary, and is only used for explaining the present invention, and is not considered as limiting the invention.
According to an aspect of the present invention, the present invention provides a kind of Thermo-sensitive biosensors.With reference to figure 1, according to this
The Thermo-sensitive biosensor is explained, the biography in the embodiment of invention (herein also referred to as " sensor ")
Sensor includes：Liquid crystal molecule 100, parent dna molecule 200 and connection DNA 300.Wherein, parent dna molecule 200 has hydrophobic
End and water-wet side, hydrophobic side are connected with liquid crystal molecule 100；Connect the both ends parent with parent dna molecule 200 respectively of DNA 300
Water end (W.E.) is connected.
In order to facilitate understanding, there is enough parent dna molecules and connection DNA in the environment to liquid crystal droplet first below
When the situation of change of the refracting power to light that can occur illustrate.Specifically, with reference to figure 2, where liquid crystal droplet
Environment present in parent dna molecule and when connection DNA lazy weights, in the microscopical white light visual field (with reference to figure 2A) and
In the polarisation visual field (with reference to figure 2B), the Liquid Crystal Molecules Alignment in liquid crystal droplet is bipolar state (with reference to figure 2C), wherein Tu2AHe
What arrow indicated is the position of liquid crystal droplet defect in 2B, i.e. small circle position in 2E and 2F figures.And it is sufficient when existing in environment
When the parent dna molecule and connection DNA of amount, the oriented of liquid crystal molecule changes in liquid crystal droplet, so as to cause liquid crystal
Drop changes to the refracting power of light, at this time in the microscopical white light visual field (with reference to figure 2D) and the polarisation visual field (ginseng
Examine Fig. 2 E) in, the Liquid Crystal Molecules Alignment in liquid crystal droplet is radiation regimes (with reference to figure 2F).Therefore, 2 and there is in the environment energy
When enough connection DNA specifically bound with above-mentioned parent dna molecule, connection DNA by with parent dna molecule in conjunction with by tie
It closes in the liquid crystal droplet, and then the arrangement mode of liquid crystal molecule in the liquid crystal droplet is caused to change, the refraction to light
Ability changes, and this change can come out conveniently by polarized light microscope observing, and then the polymerization of DNA is connected by control
The response to temperature is realized with depolymerization.
Further, the Thermo-sensitive biosensor illustrates the principle of temperature-responsive in conjunction with Fig. 3, due to connection
DNA is high to the susceptibility of its fusing point, and the variation of environment temperature can be sensitively reflected by the polymerization and depolymerization that connect DNA, into
And parent dna molecule is connected on liquid crystal molecule by inventor, recycles connection DNA connection parent dna molecules, passes through control
Connect the variation of the polymerization and depolymerization reflection environment temperature of DNA.When temperature increases to over predetermined temperature, that is, connect DNA fusing points, example
It is 80-85 DEG C such as to connect DNA fusing points, and when being heated to the temperature, connection DNA will appear fracture so as to cause liquid crystal droplet from reunion
State becomes dispersity, and when temperature drops below predetermined temperature, liquid crystal droplet can be changed into reunion from dispersity again
State, to by the state for observing liquid crystal droplet, understand the variation of temperature.
In turn, the Thermo-sensitive biosensor is high using connection DNA temperature sensitivities, and by adjusting connection DNA's
The characteristics of sequence and length can adjust fusing point, can be as needed, adjusts the reaction temperature of Thermo-sensitive biosensor, and
Sensitivity and accuracy are high.
It should be noted that the concrete type of liquid crystal molecule and parent dna molecule is not particularly limited, as long as the two energy
Enough self assemblies effective under these conditions simultaneously form liquid crystal droplet.According to an embodiment of the invention, liquid crystal molecule can be
Liquid crystal molecule with liquid crystalline phase at room temperature.Contain refraction situation of the liquid crystal droplet to light of above-mentioned liquid crystal molecule as a result,
It at normal temperatures can be with surfactant in environment (parents' molecule and the determinand that can be combined with parents' molecular specificity
Matter) content change and change, to the liquid crystal droplet can not heat or the environment of cooling treatment in complete to be measured
The application of the liquid crystal droplet is convenient in the detection of object.According to a preferred embodiment of the invention, liquid crystal molecule is selected from 5CB liquid crystal point
At least one of son, TL205 liquid crystal molecules and E7 liquid crystal molecules.Wherein, 5CB is a kind of common nematic liquid crystal, E7 and
TL205 is two kinds of mixed liquid crystals being commercially available, and E7 is by 4- normal heptanes -4 '-cyanobiphenyl (7CB), 4- pentanes -
Just pungent cyano-the 4 '-cyanobiphenyl (80CB) of 4 '-cyanobiphenyls (5CB), 4- and 4- n-pentyls -4 '-cyano terphenyl (5CT) are pressed
The nematic liquid crystal of certain proportion composition.TL205 is a kind of mixing thermotropic liquid crystal containing F.Liquid crystal molecule has at room temperature as a result,
There is liquid crystalline phase, and liquid crystal droplet quantity is more, homogeneity is good.
It should be noted that in the present invention, term " room temperature ", " room temperature " refer in particular to additionally carry out heating or cold
But the environment temperature handled, and its actual temp range is not particularly limited.For example, " room temperature ", " room temperature " are under normal conditions
The environment temperature when operations such as preparation and analyte detection to be measured is carried out, can be 10~30 DEG C specifically.
According to an embodiment of the invention, the structure of the hydrophobic side of parent dna molecule is not particularly limited, as long as energy and liquid
Brilliant drop combines.According to some embodiments of the present invention, hydrophobic side is fat chain.The hydrophobicity of fat chain is strong, and steric hindrance is small,
It is inserted easily into the gap of liquid crystal droplet, parent dna molecule is connected to liquid crystal droplet.Preferred implementation according to the present invention
Example, hydrophobic side is alkyl chain.The structure of alkyl chain is simple as a result, easily prepared, and the hydrophobicity of its alkyl chain is good, and space
Steric hindrance smaller, it is easier to be inserted into the gap of liquid crystal droplet, parent dna molecule is connected to liquid crystal droplet.
It should be noted that in the present invention, the specific type of DNA double affinity molecule is not particularly limited.Art technology
Personnel can be according to the specific type of liquid crystal molecule, and the case where needing the determinand detected selects DNA double parent appropriate point
Son.For example, according to a particular embodiment of the invention, linear liquid crystal molecule 5CB can be selected as liquid crystal molecule, select DNA-
C18, DNA-G2CL, DNA-PS or DNA-PPO are as the single-stranded parents' molecules of DNA.Selected DNA sequence dna can be (TGG
TGA AGT AGA TGT GTA, SEQ ID NO：9).
According to an embodiment of the invention, water-wet side is the single strand dna with same end.Single strand dna as a result,
It is easy to be combined by base pair complementarity with connection DNA, in turn, the reunion and dispersion of liquid crystal molecule is controlled by connecting DNA.
According to an embodiment of the invention, both ends and the water-wet side for connecting DNA are complementary.Single strand dna is easy to and connects as a result,
DNA is met to combine by base pair complementarity, and combine effect it is good, in turn, by connect DNA control liquid crystal molecule reunion and
Dispersion.
According to an embodiment of the invention, the complementary series control response temperature that Thermo-sensitive biosensor passes through connection DNA.
The sequence of length and base that the complementary series of DNA is connected by adjusting is the fusing point of changeable connection DNA, for example, sequence
Length is longer, and fusing point is higher, and the content of G and C is higher in sequence, then the fusing point of DNA is higher.
According to some embodiments of the present invention, the response temperature of Thermo-sensitive biosensor is 20-85 DEG C.In the temperature model
In enclosing, the fusing point for connecting DNA is easy to be accurately controlled, to be sensitivity and the accuracy higher of Thermo-sensitive biosensor.
According to some embodiments of the present invention, when connection DNA has nucleotide sequence as described below, the fusing point of DNA is connected
It it is 65 DEG C, then the response temperature of Thermo-sensitive biosensor is 80-85 DEG C.
R1：TCT ATT CGC ATG AGA ATT CCA TTC ACC GTA AG TAC ACA TCT ACTTCA CCA
(SEQ ID NO：1)
R2：CTT ACG GTG AAT GGA ATT GTC ATG CGA ATA GA TAC ACA TCT ACT TCA CCA
(SEQ ID NO：2)
According to some embodiments of the present invention, when connection DNA has nucleotide sequence as described below, the fusing point of DNA is connected
It it is 50 DEG C~55 DEG C, then the response temperature of Thermo-sensitive biosensor is 50 DEG C~55 DEG C.
R3：TCT ATT CGC ATG AGA ATT CCA TTC ACC GTA AG TAC ACA TCT A(SEQ ID
NO：3)
R4：CTT ACG GTG AAT GGA ATT GTC ATG CGA ATA GA TAC ACA TCT A(SEQ ID
NO：4)
According to some embodiments of the present invention, when connection DNA has nucleotide sequence as described below, the fusing point of DNA is connected
It it is 40 DEG C, then the response temperature of Thermo-sensitive biosensor is 40 DEG C.
R5：TCT ATT CGC ATG AGA ATT CCA TTC ACC GTA AG TAC ACA TC(SEQ ID NO：5)
R6：CTT ACG GTG AAT GGA ATT GTC ATG CGA ATA GA TAC ACA TC(SEQ ID NO：6)
According to some embodiments of the present invention, when connection DNA has nucleotide sequence as described below, the fusing point of DNA is connected
It it is 17-23 DEG C, then the response temperature of Thermo-sensitive biosensor is 17-23 DEG C.
R7：TCT ATT CGC ATG AGA ATT CCA TTC ACC GTA AG TACGCA TC(SEQ ID NO：7)
R8：CTT ACG GTG AAT GGA ATT GTC ATG CGA ATA GA TAC GCA TC(SEQ ID NO：8)
Below with reference to specific embodiment, the present invention will be described, it should be noted that these embodiments are only explanation
Property, and be not considered as limiting the invention.
The solution of the present invention is explained below in conjunction with embodiment.It will be understood to those of skill in the art that following
Embodiment is merely to illustrate the present invention, and should not be taken as limiting the scope of the invention.Particular technique or item are not specified in embodiment
Part, it is carried out according to technology or condition described in document in the art or according to product description.Agents useful for same or instrument
Production firm person is not specified in device, and being can be with conventional products that are commercially available, such as can purchase from Sigma companies.
Embodiment 1
Liquid crystal droplet is prepared using cell crushing instrument, and assembles and forms Thermo-sensitive biosensor, and observes the sensor
At a certain temperature, the optical change of polarized light microscope observing is utilized.
(1) 10 μ L 5CB liquid crystal droplets are added in 50 μ L, 20 μM of DNA double affinity molecule DNA-C18, use clasmatosis
20% power of instrument emulsifies 30s to prepare liquid crystal droplet.Using the form of micro- sem observation liquid crystal droplet, as shown in figure 4, being prepared into
The liquid crystal droplet Numerous arrived, size are relatively uniform.
(2) it takes 10 μ L liquid crystal droplets prepared by step (1) to be placed in low suction PC pipes, adds 10 μ L, 20 μM of DNA
linker(R1+R2) solution, it stands 5min or so and it is allowed independently to fill, form Thermo-sensitive biosensor, wherein DNA linker
(R1+R2) there is SEQ ID NO：Nucleotide sequence shown in 1 and 2, the fusing point T of the DNA linkermCurve is as shown in fig. 6, molten
Point is 65 DEG C or so.
(3) solution in step (1) and (2) is taken respectively, dilutes 50 times, by polarized light microscope observing experimental phenomena, such as
Shown in Fig. 5, wherein Fig. 5 A are the liquid crystal droplet light field figure of step (1), and Fig. 5 B are the polarisation figure of the liquid crystal droplet of step (1), figure
5C is the liquid crystal droplet photo of step (1), and Fig. 5 D are the light field figure of the Thermo-sensitive biosensor of the coherent condition of step (2),
Fig. 5 E are the polarisation figure of the Thermo-sensitive biosensor of the coherent condition of step (2), and Fig. 5 F are the temperature of the coherent condition of step (2)
Quick property biosensor photo.
(4) the Thermo-sensitive biosensor for the state of aggregation for obtaining step (2) is passed through using heating water bath to 80-85 DEG C
Polarized light microscope observing experimental phenomena, as shown in Fig. 5 G-I, wherein Fig. 5 G are the Thermo-sensitive biosensor recovery room after heating
Light field figure after temperature, Fig. 5 H are that the Thermo-sensitive biosensor after heating restores the polarisation figure after room temperature, and Fig. 5 I are after heating
Thermo-sensitive biosensor photo.
(5) it after the temperature of the Thermo-sensitive biosensor of step (4) restores room temperature, is tested by polarized light microscope observing
Phenomenon.As shown in Fig. 5 G, 5H, when experienced heating be intended to coherent condition liquid crystal molecule separated process after, wait for that solution is restored to
After room temperature, liquid crystal droplet can be assembled again.
In addition, Fig. 7 shows the photo of each stage Thermo-sensitive biosensor, in conjunction with Fig. 5 and 7 pair of each stage Thermo-sensitive life
The state of object sensor further illustrates that the liquid crystal droplet (Fig. 5 A, 5B and 7A) prepared is uniformly dispersed, after connection DNA is added,
Aggregation (Fig. 7 B, 5F, 5D and 5E) occurs for liquid crystal droplet, and precipitates；Liquid crystal droplet is heated, when being heated to 85 DEG C
When, dispersity (Fig. 7 C) is presented in liquid crystal droplet.After temperature is restored to room temperature, the liquid crystal droplet partly disperseed is had again
Clustering phenomena occurs, so can find the liquid crystal droplet (Fig. 7 D, 5G and 5H) for still there are many clustering phenomenas under white light and polarisation.
The above results show to increase to over connection DNA fusing points when temperature, and connection DNA fusing points are 65 DEG C of left sides in the present embodiment
The temperature on the right side, connection DNA depolymerization is 80-85 DEG C, and when being heated to 85 DEG C, connection DNA will appear fracture so as to cause liquid crystal droplet
Become dispersity from aggregating state, when temperature drops to room temperature, liquid crystal droplet can be changed into reunion shape from dispersity again
State, to by the state for observing liquid crystal droplet, understand the variation of temperature.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or spy described in conjunction with this embodiment or example
Point is included at least one embodiment or example of the invention.In the present specification, schematic expression of the above terms are not
Centainly refer to identical embodiment or example.Moreover, particular features, structures, materials, or characteristics described can be any
One or more embodiments or example in can be combined in any suitable manner.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that：Not
In the case of being detached from the principle of the present invention and objective a variety of change, modification, replacement and modification can be carried out to these embodiments, this
The range of invention is limited by claim and its equivalent.
Claims (9)
1. a kind of Thermo-sensitive biosensor, which is characterized in that including：
Liquid crystal molecule；
Parent dna molecule, the parent dna molecule have hydrophobic side and water-wet side, the hydrophobic side and the liquid crystal molecule phase
Even；And
DNA is connected, the both ends of the connection DNA are connected with the water-wet side of the parent dna molecule respectively.
2. Thermo-sensitive biosensor according to claim 1, which is characterized in that the liquid crystal molecule has at room temperature
Liquid crystalline phase.
3. Thermo-sensitive biosensor according to claim 2, which is characterized in that the liquid crystal molecule is selected from 5CB liquid
At least one of brilliant molecule, TL205 liquid crystal molecules and E7 liquid crystal molecules.
4. Thermo-sensitive biosensor according to claim 1, which is characterized in that the hydrophobic side is fat chain.
5. Thermo-sensitive biosensor according to claim 4, which is characterized in that the hydrophobic side is alkyl chain.
6. Thermo-sensitive biosensor according to claim 1, which is characterized in that the water-wet side is with same end
Single strand dna.
7. Thermo-sensitive biosensor according to claim 6, which is characterized in that it is described connection DNA both ends with it is described
Water-wet side is complementary.
8. Thermo-sensitive biosensor according to claim 1, which is characterized in that the Thermo-sensitive biosensor passes through
The complementary series control response temperature of the connection DNA.
9. Thermo-sensitive biosensor according to claim 1, which is characterized in that the sound of the Thermo-sensitive biosensor
It is 20-85 DEG C to answer temperature.
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| CN108020258A (en) * | 2017-11-09 | 2018-05-11 | 清华大学 | The method for preparing pattern visual under polarisation |
| CN108318061B (en) * | 2018-01-05 | 2021-03-02 | 京东方科技集团股份有限公司 | Liquid crystal sensor and detection method thereof |
| CN113560595B (en) * | 2021-08-06 | 2022-07-01 | 山东大学 | Preparation method and application of metal-based DNA thermotropic liquid crystal |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2145224A (en) * | 1983-08-16 | 1985-03-20 | Christopher John Dennis | Thermochromic liquid crystal devices |
| GB2235532A (en) * | 1989-07-28 | 1991-03-06 | Liquid Crystal Devices Ltd | A liquid crystal visual display device |
| CN1314994A (en) * | 1998-06-26 | 2001-09-26 | 医疗指示器公司 | Liquid crystal thermometer |
| CN102334018A (en) * | 2009-02-27 | 2012-01-25 | 辛根塔有限公司 | Sensor |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090263908A9 (en) * | 2007-03-07 | 2009-10-22 | Michi Nakata | Liquid crystal condensation of nucleic acid (na) complexes |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2145224A (en) * | 1983-08-16 | 1985-03-20 | Christopher John Dennis | Thermochromic liquid crystal devices |
| GB2235532A (en) * | 1989-07-28 | 1991-03-06 | Liquid Crystal Devices Ltd | A liquid crystal visual display device |
| CN1314994A (en) * | 1998-06-26 | 2001-09-26 | 医疗指示器公司 | Liquid crystal thermometer |
| CN102334018A (en) * | 2009-02-27 | 2012-01-25 | 辛根塔有限公司 | Sensor |
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