CN106525728B - Liquid crystal droplet and preparation method, biosensor and the method for detecting biological sample - Google Patents
Liquid crystal droplet and preparation method, biosensor and the method for detecting biological sample Download PDFInfo
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- CN106525728B CN106525728B CN201610890466.2A CN201610890466A CN106525728B CN 106525728 B CN106525728 B CN 106525728B CN 201610890466 A CN201610890466 A CN 201610890466A CN 106525728 B CN106525728 B CN 106525728B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/21—Polarisation-affecting properties
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
Abstract
The invention proposes liquid crystal droplet and preparation methods, biosensor and the method for detecting biological sample.This method comprises: by liquid crystal molecule and parents' molecular mixing to form mixture；And ultrasonic wave emission source is placed in the mixture, direct ultrasonic emulsification handles the mixture, to obtain the liquid crystal droplet.Thus, it is possible to make liquid crystal molecule and parents' molecule that effective assembling occur, the liquid crystal droplet quantity of acquisition is more, homogeneity is good, can carry out quick, sensitive, effective detection with the substance in conjunction with parents' molecule to liquid crystal droplet institute in the environment.
Description
Technical field
The present invention relates to Material Fields, in particular it relates to liquid crystal droplet and preparation method, biosensor and inspection
The method of sample.
Background technique
Liquid crystal state is a kind of between solid crystals and unordered liquid " soft polymorphic material ".Liquid crystal molecule has with long-range
Sequence and optical anisotropy have been widely used in plane display field.Univ Wisconsin-Madison USA Abbott is ground within 1998
Study carefully group and construct biosensor using liquid crystal molecule for the first time, opens liquid crystal applications frontier.Liquid crystal biosensor is easy to real
Now micromation and array have the advantages that structure is simple, detection is easy to be quick, pacify in life science, clinical medicine and food
Full field presents wide application prospect.The basic principle of Liquid Crystal Sensor is: before and after detecting target compound, liquid crystal point
Son is due to occurring change in orientation, to change liquid crystal molecule to the refracting power of light, using polarisation in conjunction with target compound
The change in refraction of microscope detection liquid crystal molecule can realize the detection to determinand molecule.
However, preparing the method for liquid crystal droplet at present and liquid crystal biosensor still has much room for improvement.
Summary of the invention
The application is to be made based on inventor to the discovery of following facts and problem and understanding:
Currently with the liquid crystal droplet with sensing function prepared by conventional method, it is usually present following problems: acquisition
Liquid crystal droplet size is uneven, quantity is inadequate, leads to not observe enough liquid crystal droplets in the visual field of polariscope, into
And influence sensing detection effect；When there is determinand in the environment, the change in orientation of liquid crystal droplet is unobvious, i.e., can not be in polarisation
Microscopically observation to liquid crystal droplet significant change, to influence to detect.Inventor passes through many experiments and further investigation
It was found that this is because current liquid crystal droplet preparation method can not effectively be such that liquid crystal molecule is formed, size is uniform, quantity is sufficient
Drop usually passed using the liquid crystal droplet for preparing with having also, when needing to carry out bio-sensing using liquid crystal droplet
The parents' molecular mixing for feeling function makes the liquid crystal molecule self assembly in above-mentioned parents' molecule and liquid crystal droplet, and being formed has parents
The liquid crystal droplet of molecule.However effective group can not be formed in current liquid crystal droplet between liquid crystal molecule and parents' molecule
Dress, or it is capable of forming the liquid crystal droplet number deficiency effectively assembled, and then resulting in liquid crystal droplet can not be in its environment
Determinand carry out it is sensitive, quickly and effectively detect.
The present invention is directed to solve at least some of the technical problems in related technologies.
In the first aspect of the present invention, the invention proposes a kind of methods for preparing liquid crystal droplet.Reality according to the present invention
Example is applied, this method comprises: by liquid crystal molecule and parents' molecular mixing to form mixture；And ultrasonic wave emission source is set
In the mixture, direct ultrasonic emulsification handles the mixture, to obtain the liquid crystal droplet.Thus, it is possible to make
Effective assembling occurs for liquid crystal molecule and parents' molecule, and the liquid crystal droplet quantity of acquisition is more, homogeneity is good, can be to the liquid crystal
Drop institute in the environment, can carry out quick, sensitive, effective detection with the substance in conjunction with parents' molecule.
According to an embodiment of the invention, the liquid crystal molecule has liquid crystalline phase at room temperature.The liquid crystal droplet can be with as a result,
Do not heat or the environment of cooling treatment in complete detection to determinand, consequently facilitating the application of the liquid crystal droplet.
According to an embodiment of the invention, parents' molecule include selected from bio-sensing parents molecule, SDS, CTAB and
At least one of DTAB.Above-mentioned parents' molecule can be assembled effectively with liquid crystal molecule, and assemble the liquid crystal droplet formed
Refraction of the liquid crystal droplet to light can be enable with when the substance in conjunction with above-mentioned parents' molecule by existing in the environment
Significant changes occur for power, to be conducive to be applied to sensing detection field using the liquid crystal droplet that this method obtains.
According to an embodiment of the invention, the bio-sensing parents molecule includes being selected from the single-stranded parents' molecule of DNA and phosphorus
At least one of rouge.Thus, it is possible to carry out the sensing detection of biological sample using the liquid crystal droplet.
According to an embodiment of the invention, in the mixture, the volume ratio of the liquid crystal molecule and parents' molecule is
(1:3)~(1:8).Thus, it is possible to further increase the homogeneity and quality for the liquid crystal droplet to be formed.
According to an embodiment of the invention, the frequency of the ultrasonic emulsification processing is 2KH~20KH.Thus, it is possible to further
Improve efficiency and effect that liquid crystal molecule and parents' molecule are assembled.
According to an embodiment of the invention, the time of the ultrasonic emulsification processing is 15~45s.Thus, it is possible to make liquid crystal point
Effective assembling occurs for son and the single-stranded parents' molecule of DNA, so as to further increase acquisition liquid crystal droplet quality.
According to an embodiment of the invention, the ultrasonic emulsification processing is realized using ultrasonic cell disruption instrument.As a result,
It can easily make liquid crystal molecule and parents' molecule that effective assembling occur, the liquid crystal droplet quantity of acquisition is more, homogeneity is good.
In another aspect of this invention, the invention proposes a kind of liquid crystal droplets.The liquid crystal droplet is using noted earlier
Method preparation.There is in the liquid crystal droplet liquid crystal molecule and parents' molecule effectively assembled, the liquid crystal droplet as a result,
Homogeneity it is good, can be to quick, sensitive, effective detection can be carried out with the determinand in conjunction with parents' molecule.
In still another aspect of the invention, the invention proposes a kind of biosensors.The biosensor includes liquid crystal liquid
Drop, which prepared by mentioned-above method.Thus, it is possible to that can divide with the parents contained in liquid crystal droplet
The determinand that son combines carries out quick, sensitive, effective detection.
In still another aspect of the invention, the invention proposes a kind of methods of test sample.According to an embodiment of the invention,
This method comprises: (1) provides liquid crystal droplet, the liquid crystal droplet is prepared using mentioned-above method；(2) aobvious in polarisation
The optical signalling of micro- microscopic observation liquid crystal droplet；(3) sample is mixed with the liquid crystal droplet；And (4) inclined
The optical signalling of the liquid crystal droplet of the viewed under light microscopy Jing Guo the mixing, and with without the mixed liquid crystal liquid
The picture of drop is compared, to detect the sample.Since this method is complete using the liquid crystal droplet of previously described method preparation
At detection, therefore can be easily by being observed under polariscope, quick, sensitive, effective completion is to sample to be tested
Detection.
Detailed description of the invention
Fig. 1 shows the liquid crystal droplet in the present invention to the variation schematic illustration of light refraction situation；
Fig. 2 shows the microscope photo of according to embodiments of the present invention 1 liquid crystal droplet；
The liquid crystal droplet and the microscope photo before and after the single-stranded chain combination of DNA that Fig. 3 shows according to embodiments of the present invention 1；
Fig. 4 shows the microscope photo of the liquid crystal droplet of comparative example 1 according to the present invention；
Fig. 5 shows the microscope photo of the liquid crystal droplet of comparative example 2 according to the present invention；And
Fig. 6 shows the schematic illustration of the liquid crystal droplet of the embodiment of the present invention 1 Yu the single-stranded chain combination of DNA.
Specific embodiment
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings.Below with reference to
The embodiment of attached drawing description is exemplary, it is intended to is used to explain the present invention, and is not considered as limiting the invention.
In the first aspect of the present invention, the invention proposes a kind of methods for preparing liquid crystal droplet.The liquid of this method preparation
Brilliant drop has size uniformity, the liquid crystal droplet quantity of acquisition more, yield is big, can effectively make parents' molecule and liquid crystal molecule into
At least one of the advantages that row assembles, and can be used for sensing detection.Specifically, according to an embodiment of the invention, this method comprises:
By liquid crystal molecule and parents' molecular mixing, ultrasonic wave emission source is placed in said mixture, which is carried out direct
Ultrasonic emulsification processing, to obtain liquid crystal droplet said mixture.Thus, it is possible to which liquid crystal molecule and parents' molecule occurs
Effective assembling, the liquid crystal droplet quantity of acquisition is more, homogeneity is good, can to the liquid crystal droplet in the environment can be with parents
What molecule combined carries out quick, sensitive, effective detection to determinand, wherein determinand be can in liquid crystal droplet
The substance that combines of parents' molecule.
It should be noted that in the method, the liquid crystal molecule of selection and the concrete type of parents' molecule be not by special
Limitation, as long as the two effective self assembly and can form liquid crystal droplet under the above conditions.For example, reality according to the present invention
Example is applied, liquid crystal molecule can be at room temperature with the liquid crystal molecule of liquid crystalline phase.Contain the liquid crystal liquid of above-mentioned liquid crystal molecule as a result,
Dripping at normal temperature can be with (the parents' molecule and can be special with parents' molecule of surfactant in environment to the refraction situation of light
It is anisotropic in conjunction with substance) content change and change, so that the liquid crystal droplet can not heat or the environment of cooling treatment
Detection of the middle completion to determinand, convenient for the application of the liquid crystal droplet.It should be noted that in the present invention, term " room temperature ",
" room temperature " refers in particular to not need the environment temperature for additionally carrying out heating or cooling treatment, and its actual temp range is not limited especially
System.For example, " room temperature ", " room temperature " to the environment temperature carried out when preparation and analyte detection to be measured etc. operate under normal conditions, tool
Body, it can be 10~30 degrees Celsius.
In order to facilitate understanding, there is enough surfactants (parents' molecule in the environment to liquid crystal droplet first below
And the determinand that can be combined with it) when the situation of change of the refracting power to light that can occur be illustrated.Tool
Body, with reference to Fig. 1, when enough surfactants are not present in the environment where liquid crystal droplet, regarded in microscopical white light
In wild (referring to Figure 1A) and the polarisation visual field (referring to Figure 1B), the Liquid Crystal Molecules Alignment in liquid crystal droplet is the (reference of bipolar state
Fig. 1 C), there is the distinctive characteristics defect of bipolar state (in Figure 1A and Figure 1B at arrow meaning, defect).And when in environment
There are when enough surfactants, the oriented of liquid crystal molecule changes in liquid crystal droplet, so as to cause liquid crystal droplet
It changes to the refracting power of light, at this time in the microscopical white light visual field (referring to Fig. 1 D) and the polarisation visual field (with reference to figure
In 1E), the Liquid Crystal Molecules Alignment in liquid crystal droplet is radiation regimes (referring to Fig. 1 F).Therefore, the method preparation that the present invention uses
Liquid crystal droplet, suitable parents' molecule and liquid crystal molecule carried out by assembling by one-step method and form liquid crystal droplet, and work as environment
In have can be specifically bound with above-mentioned parents' molecule determinand when, determinand is tied and in conjunction with parents' molecule
It closes in the liquid crystal droplet, equivalent to increase the content of the surfactant in liquid crystal droplet environment, and then leads to the liquid crystal
The arrangement mode of liquid crystal molecule changes in drop, changes to the refracting power of light, and this change is led in which can be convenient
It crosses polarized light microscope observing to come out, and then realizes the detection to determinand.It should be noted that in the method, term " system
It is standby " it shall be understood in a broad sense, as long as containing liquid crystal droplet in the substance being prepared using this method.In other words, originally
Invention proposes a kind of method for obtaining liquid crystal material, and can be obtained using method proposed by the invention can be with surface-active
Agent (parents' molecule and can be with the substance in conjunction with parents' molecule) is combined, and combines front and back that the refractive index of light occurs
The liquid crystal droplet of change.The liquid crystal droplet can disperse in the solution, in actual use, without to the liquid in the solution
Solvent in brilliant drop and solution is separated, and the above-mentioned of the liquid crystal droplet can be realized and use function.In the present invention, sharp
" liquid crystal droplet " prepared in aforementioned manners refers in particular to liquid crystal molecule and the substance obtained after assembling, the object occur for parents' molecule
Matter can be containing multiple liquid crystal molecules and parents' molecule assembled therewith, and parents divide the liquid crystal droplet in the environment
Son can change to the refracting power of light with when the substance in conjunction with parents' molecular specificity.And liquid crystal droplet
Contained in liquid crystal molecule, parents' molecule amount be not particularly limited.
According to an embodiment of the invention, parents' molecule may include selected from bio-sensing parents molecule, SDS (dodecyl
Sodium sulfonate), at least one of CTAB (cetyl trimethylammonium bromide) and DTAB (dodecyl trimethyl ammonium bromide).On
Stating parents' molecule can effectively assemble with liquid crystal molecule, and assembling that the liquid crystal droplet formed exists in the environment can be with
When the substance that above-mentioned parents' molecule combines, it can make the liquid crystal droplet that significant changes occur to the refracting power of light, from
And be conducive to be applied to sensing detection field using the liquid crystal droplet that this method obtains.
According to an embodiment of the invention, bio-sensing parents' molecule includes selected from the single-stranded parents' molecule of DNA and phosphatide
At least one.Thus, it is possible to carry out the sensing detection of biological sample using the liquid crystal droplet.For example, can use mono- with DNA
Chain parents' molecule carries out the detection of DNA that can be paired.
According to an embodiment of the invention, in the mixture of above-mentioned liquid crystal molecule and parents' molecule, liquid crystal molecule and double
The volume ratio of affinity molecule is 1:(3~8).For example, according to a particular embodiment of the invention, the body of liquid crystal molecule and parents' molecule
Product ratio can be 1:5.Thus, it is possible to so that suitable parents' molecule and liquid crystal molecule is carried out assembling forms liquid crystal droplet, so as to
To further increase the quality for the liquid crystal droplet to be formed.
For inventor by further investigation, the method for preparing liquid crystal droplet at present can not obtain that size is uniform, quantity is sufficient
Liquid crystal droplet, mainly since current method is usually to pass through ultrasonic wave emission source to act on intermediate medium, for example, water, so
The intermediate mediums such as water are recycled to act on containing liquid crystal molecule to obtain liquid crystal droplet afterwards.And the above process is often not able to obtain
Liquid crystal droplet of uniform size, and obtain solution in liquid crystal droplet quantity it is also extremely limited.Inventor is by further investigation
And many experiments discovery, when use ultrasonic wave emission source directly acts on the mixture containing liquid crystal molecule and parents' molecule
When (such as in the present invention, directly ultrasonic wave emission source is placed in mixture), liquid crystal molecule and parents' molecule can be sent out
Raw effective self assembly, the liquid crystal droplet quantity of acquisition is more, and size is more uniform.Inventor has found by many experiments, sharp
With traditional method (intermediate medium acts on mixture) for preparing liquid crystal droplet, even by the frequency tune of ultrasonic wave emission source
Section can not also obtain the effect that method proposed by the invention obtains to ultrasonic wave emission source of the present invention is much larger than.
According to an embodiment of the invention, the frequency of ultrasonic wave emission source can be 2KH~20KH in ultrasonic emulsification treatment process.Root
According to other embodiments of the invention, frequency can also be used for the ultrasonic wave emission source of 3KH~10KH.For example, can use
The frequency of 4KH carries out ultrasonic emulsification processing.Inventor has found by many experiments, when direct ultrasonic emulsification processing power is insufficient
When, can not be formed number enough, the liquid crystal droplet of size uniformity, and in the liquid crystal droplet formed, parents' molecule and liquid crystal molecule
Also it can not effectively fit together, to seriously affect the reliability of detection in subsequent determinand detection process.And work as
When ultrasonic emulsification processing power is excessive, then the shape that parents' molecule and liquid crystal molecule also can not be fitted together effectively can not be formed
At liquid crystal droplet.It will be appreciated to those of skill in the art that time and the ultrasonic wave emission source of above-mentioned ultrasonic emulsification processing
Frequency is related.Specifically, according to an embodiment of the invention, can be 15~45s in the time of above-mentioned ultrasonic emulsification processing.In order to
Facilitate operation, said mixture can be handled using ultrasonic cell disruption instrument.Thus, it is possible to which it is preferable that quality is easily obtained
Liquid crystal droplet.Specifically, the probe of ultrasonic cell disintegration instrument can be directly protruding into progress ultrasonic emulsification processing in mixture.
Thus, it is possible to make liquid crystal molecule and the single-stranded parents' molecule of DNA that effective assembling occur, so as to further increase acquisition
The quality of liquid crystal droplet.
It should be noted that in the present invention, the specific type of the single-stranded parents' molecule of DNA is not particularly limited.This field
Technical staff can be according to the specific type of liquid crystal molecule, and the case where needing the determinand detected selects DNA appropriate mono-
Chain parents' molecule.For example, according to a particular embodiment of the invention, can choose linear liquid crystal molecule 5CB as liquid crystal molecule, choosing
DNA-C18, DNA-G2CL, DNA-PS or DNA-PPO are selected as the single-stranded parents' molecule of DNA.Selected DNA sequence dna can be
(TGG TGA AGT AGATGT GTA)。
In another aspect of this invention, the invention proposes a kind of liquid crystal droplets.The liquid crystal droplet is using noted earlier
Method preparation.As a result, the liquid crystal droplet have using previously described method preparation liquid crystal droplet whole features with
And advantage, details are not described herein.Generally speaking, there are the liquid crystal molecule effectively assembled and parents point in the liquid crystal droplet
The homogeneity of son, the liquid crystal droplet is good, can be quick, sensitive, effective to that can carry out with the determinand in conjunction with parents' molecule
Detection.
In still another aspect of the invention, the invention proposes a kind of biosensors.The biosensor includes liquid crystal liquid
Drop, which prepared by mentioned-above method.The biosensor, which has, as a result, utilizes previously described method
The whole features and advantage of the liquid crystal droplet of preparation, details are not described herein.Generally speaking, can to can in liquid crystal droplet
The determinand that the parents' molecule contained combines carries out quick, sensitive, effective detection.Skilled artisans appreciate that
Be, in above-mentioned biosensor can also containing can be realized sensing function remaining must component, those skilled in the art can
With the specific substance detected as needed, above-mentioned necessary component is selected.
In still another aspect of the invention, the invention proposes a kind of methods of test sample.According to an embodiment of the invention,
This method comprises:
(1) liquid crystal droplet is provided
According to an embodiment of the invention, the liquid crystal droplet is prepared using previously described method.Specifically, the liquid crystal
Contain parents' molecule in drop, thus, it is possible to realize the detection to the sample that can be specifically bound with parents' molecule.
About the specific features and advantage of the liquid crystal droplet, before detailed description has been carried out, details are not described herein.
(2) liquid crystal droplet is observed
According to an embodiment of the invention, in this step, the liquid using petrographic microscope, when observing not in conjunction with determinand
The picture of brilliant drop simultaneously records, so as to subsequent contrast's use.
(3) it mixes；
According to an embodiment of the invention, in this step, sample is mixed with liquid crystal droplet.Make in sample and liquid crystal droplet
Parents' molecular specificity combine, change liquid crystal droplet in liquid crystal molecule arragement direction, to change liquid crystal droplet micro-
Picture in mirror.
(4) mixed liquid crystal droplet is passed through in observation
According to an embodiment of the invention, in this step, observe the optical signalling of above-mentioned liquid crystal droplet, and with not in contact with to
The picture of the liquid crystal droplet of sample is compared, to complete the detection of sample.When there are target lifes to be measured in determinand sample
When object sample object, the picture of liquid crystal droplet will change.
Generally speaking, detection is completed using the liquid crystal droplet of previously described method preparation due to this method, it can be with
Easily by being observed under polariscope, detection of quick, sensitive, the effective completion to sample to be tested.
The present invention will be described below by way of specific embodiments, it should be noted that specific embodiment below is only
It is to be for the purpose of illustration, without limiting the scope of the invention in any way, in addition, unless otherwise specified, then it is not specific to remember
The method of carrier strip part or step is conventional method, and used reagent and material commercially obtain.Wherein, carefully
Born of the same parents are crushed instrument and use QSonica Sonicators, NO:63736T-07-11.
Embodiment 1: the preparation of liquid crystal droplet
10 μ L 5CB are added in the single-stranded parents' molecular dna-C18 of 50 μ L, 20 μM of DNA, with 20% function of cell crushing instrument
Rate emulsifies 30s to prepare liquid crystal droplet.With reference to Fig. 2, the liquid crystal droplet Numerous being prepared, size is relatively uniform.
It is successfully assembled with liquid crystal molecule 5CB to verify parents' molecular dna-C18, in above-mentioned liquid crystal droplet
The DNA chain that can be matched with DNA-C18 is added in environment.If DNA-C18 is successfully assembled with liquid crystal molecule 5CB, then
When there is the DNA chain that can be matched with DNA-C18 in the environment, above-mentioned DNA chain can make multiple liquid crystal molecules by DNA-C18
Between occur reunite combine.Basic principle is as shown in Figure 6: the liquid crystal droplet prepared in embodiment 1 contain liquid crystal molecule (LC) with
And DNA-C18 (there are 3 ' ends and 5 ' ends, wherein 5 ' ends are connected with LC).The DNA chain structure used is with R1+R2 structure
Double-stranded DNA, wherein the end R1 and the end R2 respectively can be with a DNA-C18 complementary pairings.As a result, as prepared in embodiment 1
In liquid crystal droplet, effective assembling is had occurred in liquid crystal molecule and DNA-C18, then existing for the double-stranded DNA with R1+R2 structure
Under adjusting, above-mentioned liquid crystal droplet will reunite.
Concrete operations are as follows:
1, it takes the liquid crystal droplet prepared in 10 μ L embodiments 1 to be placed in low suction PC pipe, adds the DNA (R1 of 20 μM of 10 μ L
+ R2) solution, it stands 5min or so and it is allowed independently to fill.
2, liquid crystal droplet and the above-mentioned solution containing DNA (R1+R2) solution that respectively prepared by Example 1, lattice dilution
50 times, by polarized light microscope observing experimental phenomena, experimental result is as shown in Figure 3.Wherein, Fig. 3 a and Fig. 3 b is embodiment 1
The white light microscope picture and polarized light microscopy mirror image of the liquid crystal droplet of middle preparation；Fig. 3 c and Fig. 3 d are that DNA (R1+R2) is added
The white light microscope picture and polarized light microscopy mirror image of liquid crystal droplet.It is not difficult to find out that there is apparent agglomeration in liquid crystal droplet.
Illustrate that single stranded DNA-parents' molecular group has been mounted on liquid crystal droplet, makes complementary between single stranded DNA-parents' molecule on liquid crystal droplet
Pairing, so making liquid crystal droplet a large amount of agglomerations occur, to demonstrate single stranded DNA-parents' molecular self-assembling in liquid
On brilliant drop.
Comparative example 1
10 μ L 5CB are added to 1mL PBS to delay in filling liquid, 30 seconds ultrasounds, 30 seconds vortex concussion instruments as a cycle,
By 10-12 periods, to prepare liquid crystal droplet.Single stranded DNA parents molecular dna-is added in the liquid crystal droplet prepared
C18 assembles it with liquid crystal droplet.(Fig. 4 a is white light visual field to the MIcrosope image of the liquid crystal droplet of acquisition as shown in Figure 4
Under picture, Fig. 4 b be polarisation visual field under picture).Wherein, the number of liquid crystal droplet is obviously less, and the size of liquid crystal droplet also ten
Divide inhomogenous.
Comparative example 2
First 20 μM of single stranded DNA parents molecular dna-C18 of 10 μ L 5CB and 50 μ L are mixed, then 30 seconds ultrasounds, 30 seconds whirlpools
Concussion instrument is flowed as a cycle, by 10-12 periods, to prepare liquid crystal droplet.The microscope figure of the liquid crystal droplet of acquisition
As shown in Figure 5 (Fig. 5 a is the picture under white light visual field, and Fig. 5 b is the picture under polarisation visual field).Wherein, the number of liquid crystal droplet compared with
More than comparative example 1, but considerably less than embodiment 1, and the size of liquid crystal droplet is also very inhomogenous.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or spy described in conjunction with this embodiment or example
Point is included at least one embodiment or example of the invention.In the present specification, schematic expression of the above terms are not
It must be directed to identical embodiment or example.Moreover, particular features, structures, materials, or characteristics described can be in office
It can be combined in any suitable manner in one or more embodiment or examples.In addition, without conflicting with each other, the skill of this field
Art personnel can tie the feature of different embodiments or examples described in this specification and different embodiments or examples
It closes and combines.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, modifies, replacement and variant.
Claims (11)
1. a kind of method for preparing liquid crystal droplet characterized by comprising
By liquid crystal molecule and parents' molecular mixing to form mixture；And
Ultrasonic wave emission source is placed in the mixture, direct ultrasonic emulsification handles the mixture, described to obtain
Liquid crystal droplet.
2. the method according to claim 1, wherein the liquid crystal molecule has liquid crystalline phase at room temperature.
3. the method according to claim 1, wherein parents' molecule include bio-sensing parents molecule,
At least one of SDS, CTAB and DTAB.
4. according to the method described in claim 3, it is characterized in that, the bio-sensing parents molecule includes single-stranded selected from DNA
At least one of parents' molecule and phosphatide.
5. the method according to claim 1, wherein in the mixture, the liquid crystal molecule and the parents
The volume ratio of molecule is (1:3)~(1:8).
6. the method according to claim 1, wherein the frequency of ultrasonic emulsification processing is 2KH~20KH.
7. according to the method described in claim 6, it is characterized in that, the time of ultrasonic emulsification processing is 15~45s.
8. the method according to claim 1, wherein ultrasonic emulsification processing is using ultrasonic cell-break
What instrument was realized.
9. a kind of liquid crystal droplet, the liquid crystal droplet is prepared using according to any one of claims 1 to 88 described in any item methods.
10. a kind of biosensor, which is characterized in that including liquid crystal droplet, the liquid crystal droplet is by claim 1~8 times
The preparation of method described in one.
11. a kind of method of test sample characterized by comprising
(1) liquid crystal droplet is provided, the liquid crystal droplet is prepared using according to any one of claims 1 to 88 described in any item methods；
(2) in the optical signalling of the polarized light microscopy microscopic observation liquid crystal droplet；
(3) biological sample is mixed with the liquid crystal droplet；And
(4) in the optical signalling of the polarized light microscopy liquid crystal droplet Jing Guo the mixing under the microscope, and with without described
The picture of mixed liquid crystal droplet is compared, to detect the sample.
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