CN106520874A - Swimming bladder protein peptide having functions of reducing blood sugar and resisting oxidation and preparation method of swimming bladder protein peptide - Google Patents
Swimming bladder protein peptide having functions of reducing blood sugar and resisting oxidation and preparation method of swimming bladder protein peptide Download PDFInfo
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Abstract
The invention provides swimming bladder protein peptide having functions of reducing blood sugar and resisting oxidation and a preparation method of the swimming bladder protein peptide, and belongs to the technical field of processing of aquatic product foods. The preparation method of the swimming bladder protein peptide comprises the following steps: (1) ultrasonically processing fat-free swimming bladder slurry; (2) conducting two-step enzymatic hydrolysis with the addition of compound protease; (3) preserving enzymatic hydrolysate at high temperature and cooling the enzymatic hydrolysate to room temperature, centrifuging the enzymatic hydrolysate, reserving supernatant liquid and processing the supernatant liquid by virtue of two-step ultrafiltration; and (4) separating a filtrate by virtue of a column and collecting an elution peak so as to obtain the swimming bladder protein peptide. The method provided by the invention is simple to operate, applicable to industrial production, free from the addition of any additives, completely free from pollution, high in product yield and good in flavor; the prepared swimming bladder protein peptide has the functions of reducing blood sugar and resisting oxidation, and peptide with molecular weight less than 2000D occupies more than 95%, so that the peptide is easy for digestion and absorption; and the swimming bladder protein peptide can be widely applied to the manufacturing of special foods and nutritious foods.
Description
Technical field
The present invention relates to aquatic food processing technique field, more particularly to a kind of with hypoglycemic and anti-oxidation function
Fish maw protein peptide and preparation method thereof.
Background technology
The function of blood sugar reduction factor refer to can reduce blood sugar in diabetic patients concentration and improve the biologically active of its symptom into
Point.More natural products class sugar deductive factor, mineral matter class sugar deductive factor, vitamins sugar deductive factor are studied at present, its effect
Mechanism is different.Natural products class sugar deductive factor can be divided into flavonoids, active polysaccharide class, alkaloids, soap by chemical constitution
Glucoside, terpene, CLA, polypeptide etc..Since synthetic insulin, sulfonylurea drugs, biguanides, insulin increase
Quick dose, Glyco inhabiting agent etc. is various oral or medicine of injection is come out one after another, but chemicals often produces certain toxic and side effect,
Natural hypoglycemic composition have action temperature and and it is lasting, it is stable in properties, almost without toxic reaction, can also various sugar-lowering components simultaneously
Deposit, the advantages of comprehensively working, enjoy patient and the favor with medical circle, also become mainly grinding for the function of blood sugar reduction factor
Study carefully direction.
Have no in patent both at home and abroad at present DPP IV (dipeptidyl peptidase are prepared using air bladder resource
IV：DPP-IV) the patent literature of peptide for inhibiting.Fast development of the present invention for current fish secondary industry, fish maw protein
The quick increase of resource quantity, according to the characteristic of fish maw protein, with air bladder as raw material, by air bladder HTHP and ultrasonic wave
Deng pretreatment, using multiple protein enzyme stepwise discretization technology, separated and high performance liquid chromatography separation skill by UF membrane, gel
Art, develops while the fish maw protein peptide with specific hypoglycemic and anti-oxidation function, sets up a set of efficient multi-functional air bladder
The preparation method of protein peptides.
The content of the invention
It is to provide a kind of with hypoglycemic and anti-oxidation function fish maw protein peptide that the purpose of the present invention is.
Another object of the present invention is to provide the preparation method of above-mentioned fish maw protein peptide.
It is yet a further object of the present invention to provide the application of above-mentioned fish maw protein peptide.
In order to realize above-mentioned technical purpose, the invention provides a kind of with hypoglycemic and anti-oxidation function fish maw protein
The preparation method of peptide, comprises the following steps:
(1) the air bladder slurries of ultrasonically treated degreasing；
(2) compound protease is added to carry out two step method enzymolysis；
(3) to room temperature being cooled to after insulation under enzymolysis liquid high temperature, be centrifuged and take supernatant by two step Treatment with Ultrafiltration；
(4) filtrate crosses column chromatography for separation, collects eluting peak, obtains final product.
In above-mentioned preparation method, elder generation's Jing high-temperature process after the air bladder cleaning of step (1), then prepare air bladder slurries.The height
Temperature is processed as processing mode well known in the art, for example can be using 20-40min at 110-121 DEG C.
Step (1) ultrasonic processing method is that the air bladder slurry temperature of degreasing is adjusted to 65-75 DEG C, the supersonic frequency of 30-40kH
Rate processes 15-25min.
In embodiments of the invention, concrete operations are, from freezing air bladder, to carry out defrosting cleaning, take under the conditions of 2-8 DEG C
Air bladder, is cleaned with the clean water for meeting sanitary standard for drinking water, is incubated 20-40 after air bladder cleaning under conditions of 110-121 DEG C
Minute, then 2.0～4.0 times of water is added in air bladder, air bladder is broken into into slurry with beater, by 6000～8000g centrifugations
10～15min, goes upper-layer fat, obtains the air bladder slurries of degreasing.
In the preparation method of the fish maw protein peptide with hypoglycemic and anti-oxidation function that the present invention is provided, step (2) two
In footwork enzymolysis, the compound protease that first step enzymolysis is added is alkali protease and animal protease, and the two mass ratio is 1-
2:1, and the compound protease quality for adding is the 0.2%-0.3% of air bladder quality；
First step enzymatic hydrolysis condition is 50-55 DEG C, and pH value 7.0-8.0 digests 0.5-1.5h.
Wherein, in step (2) two step method enzymolysis, the compound protease that second step enzymolysis is added is neutral proteinase and local flavor
Protease, the two mass ratio are 1-2:1, and the compound protease quality for adding is the 0.1%-0.3% of air bladder quality；
Second step enzymatic hydrolysis condition is 50-60 DEG C, and pH value 7.0-8.0 digests 1.0-2.0h.
In above-mentioned preparation method, insulation at a high temperature of step (3) is referred to and is incubated 3-5min at 90-95 DEG C；
The centrifugation of step (3) is centrifuged 10-15min under the conditions of referring to 3000-4000g.
Two step ultrafiltrations of step (3) are first, with the film ultrafiltration supernatant liquid that aperture is 6000D, to obtain molecular weight and be less than
The filtrate of 6000D, then to the film ultrafiltration that the filtrate aperture is 3000D, obtain protein peptides of the molecular weight less than 3000D.
The concrete operations of step (4) are：It is the protein peptides liquid less than 3000 to take molecular weight, then through Sephadex G-15
Gel is separated, and eluent is deionized water, and eluting peak is detected under 280nm, is collected the 1st eluting peak, then is used RP-HPLC
RPLC carries out 1 separation, the separation condition of reversed-phase HPLC be using 5～90% acetonitrile solutions as eluent,
Take the 8-11 point of peptide solution collected.
In the preparation method that the present invention is provided, the DPP-IV for also obtaining step (4) including step (5) suppresses peptide solution to lead to
Concentration, freeze-drying are crossed, the anti-DPP-IV of air bladder is obtained and is suppressed Gly-His-Lys.By LC-MS/MS to the main of obtained fish maw protein peptide
Composition is measured, its amino acid sequence be Trp-Gly-Asp-Glu-His-Ile-Pro-Gly-Ser-Pro-Tyr-His,
Ile-Ala-Gln-Pro-Gln-Glu-Lys-Ala-Pro-Asp-Pro-Phe-Arg-His and Val-Ala-Pro-Glu-Glu-
The content of the peptide of His-Pro-Thr-Leu is 40%～60%.
The fish maw protein peptide that above-mentioned preparation method is prepared belongs to protection scope of the present invention.The present invention is sent out by experiment
Existing, the fish maw protein peptide that said method of the present invention is prepared has excellent hypoglycemic and anti-oxidation function.
Further, the invention provides the application of the fish maw protein peptide in hypoglycemic or anti-oxidation medicine is prepared.Containing this
Obtained in inventive method, the food of fish maw protein peptide, health products or medicine fall within protection scope of the present invention.
It is an advantage of the current invention that：
(1) the inventive method is raw materials used is easy to get, low cost, and controllability is strong, is suitable to industrialization production.
(2) present invention first carries out (110-121 DEG C) process of high temperature first to air bladder, at CF ultrasonic technology
The fish maw protein slurry of reason, can be obviously improved the structure of fish maw protein, improve the yield of the enzyme sensitiveness and product of fish maw protein.
(3) fish maw protein peptide obtained in the present invention has the function that preferable DPP-IV suppresses, and its IC50 value is less than
0.6mg/mL, while having preferable anti-oxidation function, under conditions of 1mg/mL, its scavenging ability of DPPH free radical reaches
More than 88%, reducing power reaches more than 0.88.
(4) fish maw protein peptide safety non-pollution prepared by the present invention, using numerous food level compound protease (basic protein
Enzyme, animal protease, neutral proteinase and flavor protease), Jing in a mild condition, obtains specific molecular through appropriateness enzymolysis
The fish maw protein peptide of amount, is not added with any additive, is 100% fish maw protein peptide.
(5) there are in present invention exploitation fish maw protein peptide three kinds of peptide (Trp-Gly-Asp-Glu-His- of specific function
Ile-Pro-Gly-Ser-Pro-Tyr-His、Ile-Ala-Gln-Pro-Gln-Glu-Lys-Ala-Pro-Asp-Pro-Phe-
Arg-His and Val-Ala-Pro-Glu-Glu-His-Pro-Thr-Leu) ratio be more than 40%.Present invention exploitation air bladder
Protein peptides middle-molecular-weihydroxyethyl is easy to body digestion for more than 95% less than the ratio of 2000 peptide and absorbs.
(6) fish maw protein peptide obtained in the inventive method, can be widely used in special procuring the manufacture of food and nutraceutical.
Specific embodiment
Unless otherwise defined, the implication that all technical terms used hereinafter are generally understood that with those skilled in the art
It is identical.Technical term used herein is intended merely to the purpose for describing specific embodiment, is not intended to limit the present invention
Protection domain.
Except there is a special instruction, the various reagents used in the present invention, raw material be commodity that can be commercially or
Person can be by product obtained in known method.
Embodiment 1 has the preparation method of the fish maw protein peptide of hypoglycemic and anti-oxidation function
(1) from 100 grams of air bladder of freezing, thawed under the conditions of 4 DEG C, with the clean water for meeting sanitary standard for drinking water
Cleaning, is incubated 20 minutes after air bladder cleaning under conditions of 121 DEG C, then adds 2.0 times of water in air bladder, will with beater
Air bladder breaks into slurry, 10～15min is centrifuged by 6000g, goes upper-layer fat, obtains the air bladder slurries of degreasing.
(2) temperature of degreasing air bladder slurries is adjusted to into 65 DEG C, (frequency is Jing ultrasonic waves in supersonic generator
40kH) process 15 minutes.
(3) add in the air bladder slurries body being ultrasonically treated according to the percentage by weight ratio of air bladder weight 0.4%
Entering compound protease carries out stepwise discretization, and the first step utilizes 0.2% compound protease (alkali protease and animal protease group
Into the mass ratio between them is 2：1), at 50 DEG C, pH be 7.5 (being adjusted with the NaOH or HCl of 1mol/L) under conditions of enter
Row enzyme digestion reaction 1.5h；Then using 0.2% compound protease (neutral proteinase and flavor protease composition, between them
Mass ratio is 2：1) enzyme digestion reaction 2.0h is carried out under the conditions of being, 7.0 (being adjusted with the NaOH or HCl of 1mol/L) in 55 DEG C, pH；
5 minutes are incubated at 95 DEG C, room temperature is cooled to, 10min clocks are centrifuged under the conditions of 4000g, supernatant is collected；
(4) molecular weight is less than into 6000 using the ceramic membrane that aperture is 6000 dalton by the supernatant obtained by step (3)
Albumen and peptide separation out, then with the film that aperture is 3000 dalton by molecular weight less than 3000 dalton protein peptides
Separate.
(5) it is the protein peptides liquid less than 3000 to take molecular weight, is separated through Sephadex G-15 gels, and eluent is to go
Ionized water, eluting peak are detected under 280nm, collect the 1st eluting peak, carried out with RP-HPLC RPLCs
Separate, the separation condition of reversed-phase HPLC is, using 5～90% acetonitrile solutions as eluent, to take the 8-11 point of peptide solution collected；
(6) DPP-IV for obtaining step (5) suppresses peptide solution by concentration, freeze-drying, obtains the anti-DPP-IV of air bladder
Suppress Gly-His-Lys.The main component of fish maw protein peptide is measured by LC-MS/MS, fish maw protein peptide obtained in the present embodiment
Middle amino acid sequence is Trp-Gly-Asp-Glu-His-Ile-Pro-Gly-Ser-Pro-Tyr-His, Ile-Ala-Gln-
Pro-Gln-Glu-Lys-Ala-Pro-Asp-Pro-Phe-Arg-His and Val-Ala-Pro-Glu-Glu-His-Pro-Thr-
The content of the peptide of Leu is 42%.
Embodiment 2 has the preparation method of the fish maw protein peptide of hypoglycemic and anti-oxidation function
(1) from 10 grams of air bladder of freezing, thawed under the conditions of 6 DEG C, with the clean water for meeting sanitary standard for drinking water
Cleaning, is incubated 40 minutes after air bladder cleaning under conditions of 110 DEG C, then adds 3.0 times of water in air bladder, will with beater
Air bladder breaks into slurry, 15min is centrifuged by 6000g, goes upper-layer fat, obtains the air bladder slurries of degreasing.
(2) temperature of degreasing air bladder slurries is adjusted to into 70 DEG C, (frequency is Jing ultrasonic waves in supersonic generator
40kH) process 25 minutes.
(3) add in the air bladder slurries body being ultrasonically treated according to the percentage by weight ratio of air bladder weight 0.5%
Entering compound protease carries out stepwise discretization, and the first step utilizes 0.2% compound protease (alkali protease and animal protease group
Into the mass ratio between them is 2：1), at 55 DEG C, pH be 7.5 (being adjusted with the NaOH or HCl of 1mol/L) under conditions of enter
Row enzyme digestion reaction 1.0h；Then using 0.3% compound protease (neutral proteinase and flavor protease composition, between them
Mass ratio is 2：1) enzyme digestion reaction 2.0h is carried out under the conditions of being, 7.5 (being adjusted with the NaOH or HCl of 1mol/L) in 55 DEG C, pH；
5 minutes are incubated at 95 DEG C, room temperature is cooled to, from 15min under the conditions of 3500g, supernatant is collected；
(4) it is by the supernatant obtained by step (3) using the ceramic membrane ultrafitration that aperture is 6000 dalton, first that molecular weight is little
In 6000 albumen and peptide separation out, then with the film that aperture is 3000 dalton by molecular weight less than 3000 dalton egg
White peptide is separated.
(5) it is the protein peptides liquid less than 3000 to take molecular weight, then is separated through Sephadex G-15 gels, and eluent is
Deionized water, eluting peak are detected under 280nm, collect the 1st eluting peak, then use RP-HPLC RPLCs
1 separation is carried out, the separation condition of reversed-phase HPLC is, using 5～90% acetonitrile solutions as eluent, to take the 8-11 point of peptide collected
Solution；
(6) DPP-IV for obtaining step (5) suppresses peptide solution by concentration, freeze-drying, obtains the anti-DPP-IV of air bladder
Suppress Gly-His-Lys.The main component of fish maw protein peptide is measured by LC-MS/MS, fish maw protein peptide obtained in the present embodiment
In its amino acid sequence be Trp-Gly-Asp-Glu-His-Ile-Pro-Gly-Ser-Pro-Tyr-His, Ile-Ala-Gln-
Pro-Gln-Glu-Lys-Ala-Pro-Asp-Pro-Phe-Arg-His and Val-Ala-Pro-Glu-Glu-His-Pro-Thr-
The content of the peptide of Leu is 43%.
Embodiment 3 has the preparation method of the fish maw protein peptide of hypoglycemic and anti-oxidation function
(1) from 1000 grams of air bladder of freezing, defrosting cleaning is carried out under the conditions of 8 DEG C, air bladder is taken, with meets water hygiene
The clean water cleaning of standard, is incubated 25 minutes after air bladder cleaning under conditions of 121 DEG C, then adds 3.0 times in air bladder
Air bladder is broken into slurry with beater by water, 15min is centrifuged by 6000g, goes upper-layer fat, obtains the air bladder slurries of degreasing.
(2) temperature of degreasing air bladder slurries is adjusted to into 70 DEG C, (frequency is Jing ultrasonic waves in supersonic generator
40kH) process 15 minutes.
(3) add in the air bladder slurries body being ultrasonically treated according to the percentage by weight ratio of air bladder weight 0.4%
Entering compound protease carries out stepwise discretization, and the first step utilizes 0.2% compound protease (alkali protease and animal protease group
Into the mass ratio between them is 1：1), at 55 DEG C, pH be 8 (being adjusted with the NaOH or HCl of 1mol/L) under conditions of carry out
Enzyme digestion reaction 1.5h；Then using 0.2% compound protease (neutral proteinase and flavor protease composition, the matter between them
Amount is than being 1：1) enzyme digestion reaction 2.0h is carried out under the conditions of being, 7.0 (being adjusted with the NaOH or HCl of 1mol/L) in 60 DEG C, pH；
5 minutes are incubated at 95 DEG C, room temperature is cooled to, 12min is centrifuged under the conditions of 4000g, collect supernatant；
(4) supernatant obtained by step (3) is processed by two step hyperfiltration process, is 6000 dalton using aperture
Ceramic membrane ultrafitration, first the albumen and peptide separation by molecular weight less than 6000 out, then with the film that aperture is 3000 dalton
Protein peptides of the molecular weight less than 3000 dalton are separated.
(5) it is the protein peptides liquid less than 3000 to take molecular weight, then is separated through Sephadex G-15 gels, and eluent is
Deionized water, collect the 1st eluting peak, then carried out with RP-HPLC RPLCs 1 time separation, reversed-phase HPLC point
It is, using 5～90% acetonitrile solutions as eluent, to take the 8-11 point of peptide solution collected from condition；
(6) DPP-IV for obtaining step (5) suppresses peptide solution by concentration, freeze-drying, obtains the anti-DPP-IV of air bladder
Suppress Gly-His-Lys.The main component of fish maw protein peptide is measured by LC-MS/MS, fish maw protein peptide obtained in the present embodiment
Middle amino acid sequence is Trp-Gly-Asp-Glu-His-Ile-Pro-Gly-Ser-Pro-Tyr-His, Ile-Ala-Gln-
Pro-Gln-Glu-Lys-Ala-Pro-Asp-Pro-Phe-Arg-His and Val-Ala-Pro-Glu-Glu-His-Pro-Thr-
The content of the peptide of Leu is 43%.
The determination test of 4 fish maw protein PEPD PP- of embodiment, IV rejection abilities
Test specimen：Fish maw protein peptide and the PEPD PP- IV of synthesis prepared by embodiment 1, embodiment 2, embodiment 3 suppresses
Ability is carried out as follows：
Sample is diluted to into suitable concentration with Tris-HCL (pH8.0) buffer solution of 100mmol/L, 25 μ L samples are drawn
Dilution is mixed with 25 μ L substrates (concentration is 1.6mmol/L), is added in 96 hole elisa Plates.After 10min is incubated at 37 DEG C,
50 μ L DPP-IV enzyme liquids (enzyme activity is 8U/L) are added, it is accurate at 37 DEG C after mixing to be incubated 60min, 100 μ L are added immediately
Acetic acid-sodium acetate (pH 4.0) the buffer solution terminating reaction of 1mol/L, surveys light absorption value A under 405nm, and according to following equation meter
Calculate the DPP-IV inhibiting rates of sample.
DPP-IV inhibiting rate %={ 1- (control of A sample-A sample blanks)/(A negative controls-A feminine gender blanks) } ×
100
A samples：The light absorption value A for being example reaction liquid at 405nm；
A sample blanks are compareed：Using the light absorption value that Tris-HCL buffer solutions replacement DPP-IV enzyme liquids are compareed as sample blank
A；
A negative controls：Sample is replaced as the light absorption value of negative control using Tris-HCL buffer solutions；
A feminine gender blanks：DPP-IV enzyme liquids and sample are replaced as negative blank using Tris-HCL buffer solutions
Light absorption value
The IC50 values that DPP-IV suppresses are determined：
DPP-IV inhibiting rate of the determination sample under variable concentrations, does recurrence with the logarithm value of peptide concentration and inhibiting rate bent
Line, obtains regression equation, is the concentration of peptide by the DPP-IV inhibition of enzyme activity of regression equation calculation IC50, i.e., 50%.As a result see
Table 1.
The determination test of 5 fish maw protein antioxidation activity of the present invention of embodiment
Test specimen：Fish maw protein peptide prepared by embodiment 1, embodiment 2, embodiment 3.
Carry out as follows：
(1) scavenging ability of DPPH free radical：Take the fish maw protein peptide 1.5mL of 1mg/mL, add 99.5% ethanol 1.5mL and
0.02%DPPH ethanol solutions 0.675mL mixes, and vibration is mixed, lucifuge water-bath 30min under room temperature, then detects under 517nm
System light absorption value.Light absorption value is lower, and the scavenging ability of DPPH free radical of system is stronger.Blank is by sample solution
1.5mL changes deionized water 1.5mL into.
DPPH radical scavenging activity %=((blank absorbency-sample light absorption value)/blank absorbency) × 100
(2) reducing power is determined：The fish maw protein peptide 1mL of 1mg/mL is taken, 0.2M phosphate buffers (pH 6.6) 2.5mL is added
With the potassium ferricyanide solution 2.5mL of 1% (mass fraction), mix, then in 50 DEG C of heating water bath 20min.Rapid cooling is taken out,
10% (mass fraction) trichloroacetic acid (TCA) solution 2.5mL is added, is well mixed, 10min is centrifuged under 3000g then.Take
Clear liquid 2.5mL, adds deionized water 2.5mL and 1% (mass fraction) liquor ferri trichloridi 0.5mL, fully mixes, at room temperature
Reaction 10min, with 700nm wavelength mensuration absorbances.Reducing power can use light absorption value at 700nm wavelength to represent.
As a result fish maw protein peptide of the present invention has good DPP-IV rejection abilities, DPP-IV inhibitory activity (to be shown in Table 1)
(IC50Value) less than 0.60mg/mL, the IC of substantially less than similar compound protein peptide50Value, with good DPP-IV rejection abilities.
The fish maw protein peptide of the present invention has preferable oxidation resistance simultaneously, and under conditions of 1mg/mL, which removes DPPH free radicals
Ability reaches more than 88%, and reducing power reaches more than 0.88, is also a kind of preferable anti-oxidation peptide.
The DPP-IV of 1 fish maw protein peptide of the present invention of table suppresses and antioxidant activity tests result
Note：The amino acid sequence Trp-Gly-Asp-Glu-His-Ile-Pro-Gly-Ser-Pro-Tyr-His of synthetic peptide 1
The amino acid sequence Ile-Ala-Gln-Pro-Gln-Glu-Lys-Ala-Pro-Asp-Pro-Phe- of synthetic peptide 2
Arg-His
The amino acid sequence Val-Ala-Pro-Glu-Glu-His-Pro-Thr-Leu of synthetic peptide 3
Embodiment above is only that the preferred embodiment of the present invention is described, and not the scope of the present invention is entered
Row is limited, on the premise of without departing from design spirit of the present invention, technical side of this area ordinary skill technical staff to the present invention
Various modifications and improvement that case is made, all should fall in the protection domain of claims of the present invention determination.
Sequence table
<110>China Agricultural University
<120>A kind of fish maw protein peptide with hypoglycemic and anti-oxidation function and preparation method thereof
<130> KHP161116973.3
<160> 3
<170> PatentIn version 3.5
<210> 1
<211> 12
<212> PRT
<213>Fish
<400> 1
Trp Gly Asp Glu His Ile Pro Gly Ser Pro Tyr His
1 5 10
<210> 2
<211> 14
<212> PRT
<213>Fish
<400> 2
Ile Ala Gln Pro Gln Glu Lys Ala Pro Asp Pro Phe Arg His
1 5 10
<210> 3
<211> 9
<212> PRT
<213>Fish
<400> 3
Val Ala Pro Glu Glu His Pro Thr Leu
1 5
Claims (10)
1. a kind of preparation method of the fish maw protein peptide with hypoglycemic and anti-oxidation function, it is characterised in that including following step
Suddenly：
(1) the air bladder slurries of ultrasonically treated degreasing；
(2) compound protease is added to carry out two step method enzymolysis；
(3) to room temperature being cooled to after insulation under enzymolysis liquid high temperature, be centrifuged and take supernatant by two step Treatment with Ultrafiltration；
(4) filtrate crosses column chromatography for separation, collects eluting peak, obtains final product.
2. preparation method according to claim 1, it is characterised in that：Elder generation's Jing high-temperature process after the air bladder cleaning of step (1),
Air bladder slurries are prepared again.
3. preparation method according to claim 1, it is characterised in that：Step (1) ultrasonic processing method is by the fish of degreasing
Fish glue slurry temperature is adjusted to 65-75 DEG C, and the supersonic frequency of 30-40kH processes 15-25min.
4. preparation method according to claim 1, it is characterised in that：In step (2) two step method enzymolysis, first step enzymolysis adds
The compound protease for entering is alkali protease and animal protease, and the two mass ratio is 1-2:1, and the compound protease matter for adding
Measure the 0.2%-0.3% for air bladder quality；
First step enzymatic hydrolysis condition is 50-55 DEG C, and pH value 7.0-8.0 digests 0.5-1.5h.
5. preparation method according to claim 1, it is characterised in that：In step (2) two step method enzymolysis, second step enzymolysis adds
The compound protease for entering is neutral proteinase and flavor protease, and the two mass ratio is 1-2:1, and the compound protease matter for adding
Measure the 0.1%-0.3% for air bladder quality；
Second step enzymatic hydrolysis condition is 50-60 DEG C, and pH value 7.0-8.0 digests 1.0-2.0h.
6. preparation method according to claim 1, it is characterised in that：Insulation at a high temperature of step (3) is referred in 90-95 DEG C
Lower insulation 3-5min；
The centrifugation of step (3) is centrifuged 10-15min under the conditions of referring to 3000-4000g.
7. according to the arbitrary described preparation method of claim 1-6, it is characterised in that：Two step ultrafiltrations of step (3) are first to use
Film ultrafiltration supernatant liquid of the aperture for 6000D, obtains filtrate of the molecular weight less than 6000D, then is 3000D's to the filtrate aperture
Film ultrafiltration, obtains protein peptides of the molecular weight less than 3000D.
8. the fish maw protein peptide that the preparation method according to any one of claim 1-7 is prepared.
9. application of the fish maw protein peptide described in claim 8 in hypoglycemic or anti-oxidation medicine is prepared.
10. containing the food of fish maw protein peptide, health products or medicine described in claim 8.
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