CN106497810B - A kind of method of germ oligotrophy unit cell, the microbial inoculum containing the bacterium and its application and diesel oil of degrading - Google Patents
A kind of method of germ oligotrophy unit cell, the microbial inoculum containing the bacterium and its application and diesel oil of degrading Download PDFInfo
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Abstract
The present invention relates to the technical field of biological remediation of petroleum hydrocarbon pollution, disclose a kind of germ oligotrophy unit cell, the microbial inoculum containing the bacterium and its application in degradation diesel oil and a kind of method for diesel oil of degrading, wherein, the deposit number of the germ oligotrophy unit cell is CGMCC NO:10846.The method of degradation diesel oil of the invention includes: to contact germ oligotrophy unit cell that deposit number is CGMCC NO:10846 and/or microbial inoculum containing the germ oligotrophy unit cell that deposit number is CGMCC NO:10846 with contaminated by diesel oil sample, with the diesel oil in contaminated by diesel oil sample of degrading.Germ oligotrophy unit cell of the invention can be effectively resistant in liquid phase and soil phase and the diesel component for high concentration of degrading, and it is not necessarily to additional carbon, it is also lower to the other elements demand such as nitrogen, phosphorus, it is applicable to the high concentration diesel bio repair process of dead soil and dystrophic industrial wastewater.
Description
Technical field
The present invention relates to the technical field of biological remediation of petroleum hydrocarbon pollution, and in particular, to a kind of thermophilic malt oligotrophy list
Born of the same parents bacterium, the microbial inoculum containing the bacterium and its application and a kind of method for diesel oil of degrading.
Background technique
Diesel oil is a kind of light petroleum hydrocarbon product that main component is C10-C24, is widely applied as vehicle, ship, power generation
The fuel of machine etc..Its complicated component, including alkane, alkene, aromatic hydrocarbon and polycyclic aromatic hydrocarbon etc., and may containing a small amount of sulphur,
Nitrogen compound.These components much have carcinogenic, teratogenesis, mutagenic property.
Diesel oil enters in soil, will lead to soil physico-chemical property and changes, destroys soil texture；Meanwhile diesel component
Root system of plant can be damaged, the respiration of plant root is hindered, to inhibit the growth of plant or lead to the death of plant；Soil
In microbes killed by the constituent part in diesel oil, cause soil microenvironment to change, the final ecology for influencing soil
Circulation ability.Human internal environment may be entered by underground water, food chain and directly contact into the diesel oil in environment, may be led
Cause respiratory system, nervous system, the circulatory system, endocrine system of human body etc. that lesion occurs.There are researcher's discovery, bavin in soil
When oil concentration is more than 0.1g/kg soil, i.e., it can cause damages to ecological environment.
And the low volatility and high-adhesiveness of diesel oil make the soil harm by contaminated by diesel oil larger, it is difficult to repair.Object
Reason, chemical method is high for the rehabilitation expense of soil, heavy workload, may cause secondary pollution, thus have safety, it is economical,
The bioremediation technology of environmentally friendly property becomes the preferable selection of diesel fuel contaminated soil.And carry out the screening of contaminated by diesel oil degradation bacteria strains
It is the biological prosthetic essential step of diesel fuel contaminated soil.
The dominant mechanism of microbial degradation diesel oil is to pass through P450The enzymes such as enzyme and enzyme system degrading polycyclic aromatic hydrocarbons, it is de- by alkane
The enzymes such as hydrogen enzyme, monooxygenase, dioxygenase and enzyme system degradation chain hydrocarbon, final product CO2And water, and energy is provided using this process
Amount carries out growing multiplication.
Currently, people have screened the microorganism, including bacterium, fungi etc. for obtaining some diesel oil that can degrade.It is false single
Some microorganisms of the fungies Pseudomonas such as the bacteria genus such as born of the same parents Pseudomonas, bacillus, Flavobacterium, acinetobacter and saccharomyces
It is all reported with Diesel degradation ability.However, both at home and abroad practical biological prosthetic in the process with higher diesel oil tolerance
And high degradation capability, and can be reported with the bacterial strain that diesel oil is single carbon source less.
Summary of the invention
The purpose of the invention is to overcome contaminated by diesel oil it is biological prosthetic present in drawbacks described above, a kind of thermophilic malt is provided
Stenotrophomonas, the microbial inoculum containing the bacterium and its application and a kind of method for diesel oil of degrading.Thermophilic malt oligotrophy unit cell of the invention
Bacterium can grow under higher diesel oil concentration, be single carbon source for growth and efficient degradation diesel oil with diesel oil.
To achieve the goals above, the present inventor has carried out a large amount of screening experiment, as a result screens a kind of tool
There are preferable diesel oil tolerance and high degradation capability, and can be the germ oligotrophy unit cell of single carbon source with diesel oil.
Therefore, in a first aspect, the present invention provides a kind of germ oligotrophy unit cell (also known as germ oligotrophy unit cell,
Stenotrophomonas maltophilia), the deposit number of the germ oligotrophy unit cell is CGMCC NO:10846.
Second aspect, the present invention provides one kind to contain above-mentioned germ oligotrophy unit cell (Stenotrophomonas
Maltophilia microbial inoculum).
The third aspect, the present invention provides the application of above-mentioned germ oligotrophy unit cell and/or microbial inoculum in degradation diesel oil.
Fourth aspect, the present invention provides a kind of methods of diesel oil of degrading, which comprises by above-mentioned thermophilic malt oligotrophy
Monad and/or above-mentioned microbial inoculum are contacted with contaminated by diesel oil sample, with the diesel oil in contaminated by diesel oil sample of degrading.
Germ oligotrophy unit cell of the invention, can effectively be resistant in liquid phase and soil phase and high concentration bavin of degrading
Oil ingredient, and the bacterium can be not necessarily to additional carbon with diesel oil for single carbon source, it is also lower to the other elements demand such as nitrogen, phosphorus,
It is applicable to the high concentration diesel bio repair process of dead soil and dystrophic industrial wastewater.
Germ oligotrophy unit cell of the invention when being single carbon source with diesel oil can normal growth breeding, and containing
Can up to degrade in 12d in the basic inorganic salt culture medium of 2.5 weight % diesel oil 51.8% diesel component, containing up to
In the soil of 2.5 weight % diesel oil can normal growth and in 16d degradation 60.8% diesel component.
Other features and advantages of the present invention will the following detailed description will be given in the detailed implementation section.
Biological deposits
Germ oligotrophy unit cell (Stenotrophomonas maltophilia) of the invention, May 22 in 2015
It is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center (address: the Chaoyang District, Beijing City North Star west day
The institute 3 of road 1, Institute of Microorganism, Academia Sinica, postcode: 100101) (depositary institution is abbreviated as CGMCC) protects
Hiding number is CGMCC:10846.
Specific embodiment
Detailed description of the preferred embodiments below.It should be understood that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to restrict the invention.
In a first aspect, the present invention provides a kind of germ oligotrophy unit cell (Stenotrophomonas
Maltophilia), the deposit number of the germ oligotrophy unit cell is CGMCC NO:10846.
Deposit number of the invention is the germ oligotrophy unit cell of CGMCC NO:10846, is Gram-negative bacteria, belongs to
γ-Proteobacteria Stenotrophomonas category germ oligotrophy unit cell.The bacterial strain causes from oil pipeline rupture at Guizhou Province one
The polluted soil of contaminated by diesel oil isolates and purifies acquisition via liquid phase concentration method.Biological characteristics are as follows: Gram-negative bacteria, it is short
It is rod-shaped, have flagellum, bacterium colony is in faint yellow needle-shaped on LB agar medium, has gelatin hydrolysis enzymatic activity, there is strong solution lipid, can
It is grown using glucose, sucrose, maltose, mannitol.
Wherein, the separation process of germ oligotrophy unit cell of the invention may include: oil transportation at the acquisition Guizhou Province 10g one
Pipeline breaking leads to the polluted soil sample of contaminated by diesel oil, and 100mL basic inorganic salt fluid nutrient medium is added, by diesel oil dirt
Pedotheque vibrating dispersion is contaminated, centrifuging and taking 1mL supernatant is simultaneously added to the basic inorganic salt that 200mL contains 0.5 weight % diesel oil
Fluid nutrient medium (the formula of basic inorganic salt fluid nutrient medium are as follows: 1.0g/L KH2PO4；5.0g/L K2HPO4；1.0g/L
(NH4)2SO4；20mg/L MgSO4·7H2O；10mg/L CaCl2·2H2O；1mg/L FeCl3；PH=6.0-7.5 in), 37
DEG C, 3d is cultivated in 170rpm shaking table.It draws 1mL culture solution and is added to the basic inorganic salt that 200mL contains 1.0 weight % diesel oil
In fluid nutrient medium, 3d is cultivated in 37 DEG C, 170rpm shaking table.This process is repeated, it is every in basic inorganic salt fluid nutrient medium
The secondary Determination of Diesel Oil for improving 0.5 weight %, until Determination of Diesel Oil is 2.5 weight % in basic inorganic salt fluid nutrient medium.It draws
200 μ L culture solutions are coated on the basic inorganic salt agar medium containing 2.5 weight % diesel oil, 37 DEG C of culture 3d.It draws and takes bacterium
It falls, and scribing line separation obtains single colonie on LB agar medium, obtains more plants of pure bacterial strains, selects wherein six plants of bacterium, orders respectively
The bacterium colony feature of entitled GZ-1, W-5, GZ-3, GZ-4, GZ-5 and GZ-6, six plants of bacterium are as shown in table 1.
Table 1
| Number | GZ-1 | W-5 | GZ-3 | GZ-4 | GZ-5 | GZ-6 |
| Size | It is medium | It is small | It is medium | It is small | It is medium | It is medium |
| Color | White | It is faint yellow | White | Yellow | White | It is faint yellow |
From the foregoing, it will be observed that six plants of separating obtained bacterium are resistant to high concentration (2.5 weight %) diesel oil, and can be with it
Single carbon source carries out growth and breeding.
Aforementioned six plants of bacterium are accessed into 37 DEG C of culture 16h in 200mL LB liquid medium respectively, centrifugation takes acclimatization,
It is washed respectively with basic inorganic salt fluid nutrient medium and precipitates and precipitating is diluted to OD600=2.5, then it is added into dilution
Diesel oil, the content for controlling diesel oil is 2.5 weight %, while blank control is arranged and (is added in basic inorganic salt fluid nutrient medium
Diesel oil, and the content for controlling diesel oil is 2.5 weight %), 12d then is cultivated in 37 DEG C, 170rpm shaking table, uses gas-chromatography
(gas chromatograph model GC-2010, be purchased from SHIMADZU company, chromatographic column be Agilent ZORBAX SB-Aq (4.6mm ×
150mm × 5 μm), mobile phase is 0.005mol/L H2SO4, flow velocity 1.0ml/min, column oven be 65 DEG C) detection diesel oil contain
Measure, calculating diesel oil removal rate, wherein diesel oil removal rate=(amount of diesel oil after amount-degradation of diesel oil before degrading)/preceding diesel oil of degrading
Amount, the results are shown in Table 2 for the diesel oil removal rate of aforementioned six plants of bacterium.
Table 2
| Number | GZ-1 | W-5 | GZ-3 | GZ-4 | GZ-5 | GZ-6 | Blank control |
| Diesel oil removal rate | 45.5% | 51.8% | 32.0% | 8.3% | 14.8% | 30.0% | 1.1% |
As shown in Table 2, bacterial strain W-5 has obvious preferable diesel oil tolerance and degradation capability in the liquid phase.
Physiology and biochemistry identification and analysis, biological characteristics are carried out to bacterial strain W-5 are as follows: Gram-negative bacteria, rod-short have whip
Hair, bacterium colony is in faint yellow needle-shaped on LB agar medium, has gelatin hydrolysis enzymatic activity, has strong solution lipid, using grape
Sugar, sucrose, maltose, mannitol growth.
The total DNA of bacterial strain W-5 is extracted simultaneously, carries out 16S rDNA gene sequencing, 16s rDNA gene order is such as
Shown in SEQ ID NO.1, the results showed that the bacterium is germ oligotrophy unit cell (Stenotrophomonas maltophilia).
The germ oligotrophy unit cell is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number is
CGMCC NO:10846.
Germ oligotrophy unit cell provided by the invention can generate the work of a large amount of germ oligotrophy unit cells by culture
Thallus, the method for the culture do not require particularly, as long as the germ oligotrophy unit cell can be made to be proliferated, example
It such as, can be according to 107The viable bacteria body of germ oligotrophy unit cell is inoculated in LB culture medium by the inoculum concentration of CFU/mL, in 30-
After cultivating 12-48 hours at a temperature of 38 DEG C, culture solution is obtained.
In the present invention, for the condition of culture of germ oligotrophy unit cell, there is no particular limitation, can be normal for this field
Various condition of culture, for example, the composition of LB liquid medium used can be with when culture are as follows: 0.8-1 weight % peptone,
0.5-0.8 weight % yeast powder, 1-1.5 weight % sodium chloride, pH=6.8-7.0.Also contain 2.5-3.0 in solid LB media
The agar of weight %.The composition of minimal medium used can be with when culture are as follows: 1.0g/L KH2PO4；5.0g/L K2HPO4；
1.0g/L(NH4)2SO4；20mg/L MgSO4·7H2O；10mg/L CaCl2·2H2O；1mg/L FeCl3；PH=6.0-7.5.
The present invention can further separate the viable bacteria body of the germ oligotrophy unit cell in above-mentioned culture solution, the separation
Method is not particularly limited, as long as thallus can be enriched with from culture solution, such as can be by being centrifuged and/or filtering
Method realizes that the condition of the centrifugation and the filtering can be well known condition, and details are not described herein by the present invention.
Second aspect, the present invention provides the germ oligotrophy unit cells for being CGMCC NO:10846 containing deposit number
The microbial inoculum of (Stenotrophomonas maltophilia).
Under preferable case, which contains the viable bacteria body of the germ oligotrophy unit cell.
In the present invention, to the concentration of germ oligotrophy unit cell described in microbial inoculum, there is no particular limitation, can be according to tool
The case where body, is specifically selected, and in this not go into detail.
In addition, different according to scheduled purposes, microbial inoculum provided by the invention can be prepared as different dosage forms, and add phase
The ingredients such as the excipient answered.Wherein, it is known to those skilled in the art which kind of excipient is added in the microbial inoculum of which kind of dosage form,
In this not go into detail.
The third aspect, the present invention provides the application of above-mentioned germ oligotrophy unit cell and/or microbial inoculum in degradation diesel oil.
Under preferable case, diesel oil source is the soil of contaminated by diesel oil or the waste water containing diesel oil.
Fourth aspect, the present invention provides a kind of methods of diesel oil of degrading, this method comprises: being CGMCC by deposit number
The germ oligotrophy unit cell of NO:10846 and/or contain the germ oligotrophy unit cell that deposit number is CGMCC NO:10846
Microbial inoculum is contacted with contaminated by diesel oil sample, with the diesel oil in contaminated by diesel oil sample of degrading.
In the method for the present invention, it can be various sides commonly used in the art that for the method for contact, there is no particular limitation
Method, for example, can be added in contaminated by diesel oil sample deposit number be CGMCC NO:10846 germ oligotrophy unit cell and/or
Microbial inoculum containing the germ oligotrophy unit cell that deposit number is CGMCC NO:10846, is uniformly mixed.Under preferable case, the sample
Product are soil or the waste water containing diesel oil.
It is not special for the form for the germ oligotrophy unit cell being added into contaminated by diesel oil sample in the method for the present invention
Other restriction, if guarantee be added after the germ oligotrophy unit cell can work in the contaminated by diesel oil sample and
It effectively degrades to diesel oil, the form of the germ oligotrophy unit cell of addition, for example, can be culture to logarithmic phase
Thallus or bacterium solution, or the thalli dry powder after freeze-drying.
The present invention is also not particularly limited the quantity of the germ oligotrophy unit cell of addition and the amount of microbial inoculum, this can be with
It is determined according to the content of the diesel oil in the contaminated by diesel oil sample and degradation complexity, for example, when in the sample
When Determination of Diesel Oil is higher or more difficult to degrade or less favorable for the existence of the germ oligotrophy unit cell, it can be improved described thermophilic
The inoculum concentration of malt Stenotrophomonas and the additional amount of microbial inoculum；When the Determination of Diesel Oil in the sample is lower or more degradable or right
When the influence of the existence of the germ oligotrophy unit cell is smaller, it is possible to reduce the inoculum concentration of the germ oligotrophy unit cell and
The additional amount of microbial inoculum.
According to the present invention, when contaminated by diesel oil sample is soil, in order to further promote thermophilic malt provided by the invention few
Monad is supported to the degradation efficiency of diesel oil, it is preferred that control the water content in soil at least 15 weight %, more preferably
18-30 weight %.
Embodiment
Below will by embodiment and comparative example, the present invention will be described in detail, but be not intended to limit the present invention.
It is conventional method in that art unless otherwise specified in experimental method in following embodiment and comparative example.It is following
Experimental material used in embodiment and comparative example is unless otherwise specified to be commercially available from routine biochemistry reagent shop.
Basic inorganic salt Liquid Culture based formulas are as follows: 1.0g/L KH2PO4；5.0g/L K2HPO4；1.0g/L(NH4)2SO4；
20mg/L MgSO4·7H2O；10mg/L CaCl2·2H2O；1mg/L FeCl3；PH=7.0.
The method that gas chromatography measures Determination of Diesel Oil includes: gas chromatograph model GC-2010, is purchased from SHIMADZU
Company, chromatographic column are Agilent ZORBAX SB-Aq (4.6mm × 150mm × 5 μm), and mobile phase is 0.005mol/L H2SO4, stream
Speed is 1.0ml/min, and column oven is 65 DEG C.
It is adopted to extract the diesel oil in pedotheque with measuring its content (g/g) according to 3546 method of U.S. EPA [123]
Diesel component is separated from pedotheque with microwave extraction method, concrete operations are as follows:
1) 2.0g (weight in wet base) pedotheque is taken, is added in microwave abstracting tank；
2) be added into microwave abstracting tank 25.0mL acetone-n-hexane mixed solution (acetone and n-hexane volume ratio are 1:
1)；
3) it will be symmetrically put into Microwave Extraction Apparatus after microwave abstracting tank proper seal, hygrosensor is connected to microwave abstracting
Tank；
4) microwave abstracting power is set as 1200W, 120 DEG C are at the uniform velocity warming up in 10min, keeps 120 DEG C of constant temperature extractings
30min；
5) after extracting, 40 DEG C are down to temperature, break-off signal detector takes out microwave abstracting tank and closes microwave abstracting
Instrument；
6) using filter paper extracted by filtration liquid of having weighed, extract liquor is collected in test tube；
7) using acetone-n-hexane mixed solution (acetone and n-hexane volume ratio be 1:1) cleaning microwave abstracting tank, with same
One filter paper filters cleaning solution and collects filtrate in test tube to measure Determination of Diesel Oil；
8) filter paper and whole soil samples are transferred to and have been weighed in triangular flask, be placed in draught cupboard, volatilize completely to solvent, weighed
To calculate pedotheque weight (dry weight), and calculate the Determination of Diesel Oil in pedotheque (g/g).
Diesel oil removal rate=(amount of diesel oil after amount-degradation of diesel oil before degrading)/amount of diesel oil before degrading
Preparation example
The germ oligotrophy unit cell that deposit number of the invention is CGMCC NO:10846 is lived in LB liquid medium
Change 2 times, activation carries out 12 hours at 170rpm, 37 ± 1 DEG C every time, bacterium solution is obtained, by obtained bacterium solution with 3 volume %'s
Inoculum concentration is inoculated in 300ml LB liquid medium, is cultivated 12 hours, is obtained under 170rpm, 37 ± 1 DEG C of condition of culture
Bacterium solution.
Embodiment 1
The liquid for the germ oligotrophy unit cell that the present embodiment is used to illustrate that deposit number of the invention is CGMCC NO:10846
Phase Diesel degradation ability.
The bacterium solution that preparation example is obtained is centrifuged, and obtains thallus, is then made with brine thallus and resuspension
OD600It is respectively the thermophilic of CGMCC NO:10846 by the deposit number of the invention of aforementioned obtained 1mL for 1 physiological saline bacterium solution
The physiological saline bacterium solution of malt Stenotrophomonas is seeded to the basic inorganic salt fluid nutrient medium that 30mL contains 2.5 weight % diesel oil
In the LB liquid medium for containing 2.5 weight % diesel oil with 30mL, 12d is cultivated in 37 DEG C, 170rpm shaking table.Use gas phase color
Spectrum detection Determination of Diesel Oil, calculates diesel oil removal rate.
The experimental results showed that the bacterium is in 12d in the basic inorganic salt fluid nutrient medium containing 2.5 weight % diesel oil
Can degrade 51.8% diesel component, therefore show deposit number of the invention be CGMCC NO:10846 thermophilic malt oligotrophy list
Born of the same parents bacterium can not only be that single carbon source is grown, and has preferable tolerance and high concentration diesel component of degrading with diesel oil
Ability.In the LB liquid medium containing 2.5 weight % diesel oil, the bacterium can degrade in 12d 32.3% diesel component,
The bacterium also has the ability of preferable tolerance and degradation high concentration diesel component i.e. in there are the liquid phase of two or more carbon sources.
Embodiment 2
The soil for the germ oligotrophy unit cell that the present embodiment is used to illustrate that deposit number of the invention is CGMCC NO:10846
Earth phase Diesel degradation ability.
500g uncontaminated soil sample is done into following processing respectively:
CK group: it is 20 weight % that deionized water to soil moisture content, which is added,；
NTC group: pedotheque is sterilized 20min at 121 DEG C, is sterilized 3 times altogether, and 1 weight is added into soil after cooling
Measure the NaN of %3Antibacterial, it is 20 weight % that deionized water to soil moisture content, which is then added, is uniformly mixed；
B1 group: the bacterium solution of aforementioned isolated GZ-1 bacterial strain is obtained according to the method for preparation example, is centrifuged, obtains bacterium
Then OD is made with brine thallus and resuspension in body600For 2.5 physiological saline bacterium solution, it is added in pedotheque
The physiological saline bacterium solution of the aforementioned obtained GZ-1 bacterial strain of 50mL, it is 20 weights that deionized water to soil moisture content, which is then added,
% is measured, is uniformly mixed；
B2 group: the bacterium solution that preparation example is obtained is centrifuged, and obtains thallus, then with brine thallus and is resuspended
OD is made600For 2.5 physiological saline bacterium solution, it is CGMCC NO that the aforementioned deposit number of the invention of 50mL, which is added, in pedotheque:
The physiological saline bacterium solution of 10846 germ oligotrophy unit cell, it is 20 weight % that deionized water to soil moisture content, which is then added,
It is uniformly mixed；
B1+B2 group: the thermophilic wheat that the aforementioned deposit number of the invention of 25mL is CGMCC NO:10846 is added in pedotheque
The physiological saline bacterium solution of the aforementioned obtained GZ-1 bacterial strain of the physiological saline bacterium solution and 25mL of bud Stenotrophomonas, then be added go from
Sub- water to soil moisture content is 20 weight %, is uniformly mixed.
Diesel oil (- 20#) to the Determination of Diesel Oil that filtration sterilization is added in the above each group is 2.5 weight %.
It is cultivated at 25 DEG C, adding deionized water to soil moisture content daily is 20 weight %, the culture of 16d is carried out, and
Diesel component is separated using microwave abstracting, gas chromatography measures final Determination of Diesel Oil, calculates diesel oil removal rate, as a result 3 institute of table
Show.
Table 3
| Number | NTC group | CK group | B1 group | B2 group | B1+B2 group |
| Diesel oil removal rate | 15.8% | 28.4% | 52.0% | 60.8% | 50.2% |
As can be seen from Table 3, deposit number of the invention is that the germ oligotrophy unit cell of CGMCC NO:10846 is containing
Up in the soil of 2.5 weight % diesel oil can normal growth and can degrade in 16d 60.8% diesel fuel composition, i.e. this hair
The germ oligotrophy unit cell that bright deposit number is CGMCC NO:10846 has the tolerance of preferable diesel oil and is dropped in soil phase
Solution ability.
The preferred embodiment of the present invention has been described above in detail, still, during present invention is not limited to the embodiments described above
Detail within the scope of the technical concept of the present invention can be with various simple variants of the technical solution of the present invention are made, this
A little simple variants all belong to the scope of protection of the present invention.
It is further to note that specific technical features described in the above specific embodiments, in not lance
In the case where shield, can be combined in any appropriate way, in order to avoid unnecessary repetition, the present invention to it is various can
No further explanation will be given for the combination of energy.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should also be regarded as the disclosure of the present invention.
Claims (6)
1. a kind of germ oligotrophy unit cell (Stenotrophomonas maltophilia), which is characterized in that the thermophilic malt
The deposit number of Stenotrophomonas is CGMCC NO:10846.
2. a kind of microbial inoculum, which is characterized in that the microbial inoculum contains germ oligotrophy unit cell described in claim 1
The viable bacteria body of (Stenotrophomonas maltophilia).
3. germ oligotrophy unit cell described in claim 1 and/or microbial inoculum as claimed in claim 2 answering in degradation diesel oil
With.
4. application according to claim 3, wherein diesel oil source is the soil of contaminated by diesel oil or the waste water containing diesel oil.
5. a kind of method for diesel oil of degrading, which is characterized in that the described method includes: by thermophilic malt oligotrophy described in claim 1
Monad and/or microbial inoculum as claimed in claim 2 are contacted with contaminated by diesel oil sample, with the diesel oil in contaminated by diesel oil sample of degrading.
6. according to the method described in claim 5, wherein, the sample is soil or the waste water containing diesel oil.
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| CN106591166B (en) * | 2015-10-19 | 2019-09-03 | 粮华生物科技（北京）有限公司 | The biology in situ preparation for repairing and restorative procedure of Bravo contaminated soil |
| CN111690559B (en) * | 2020-06-04 | 2021-09-24 | 江南大学 | Stenotrophomonas maltophilia capable of degrading polyethylene glycol terephthalate |
| CN112029689A (en) * | 2020-09-24 | 2020-12-04 | 苏州道源华智环保科技有限公司 | Acrylic resin emulsion wastewater degradation strain, culture method and application |
| CN113151124B (en) * | 2021-01-04 | 2024-01-19 | 南京工业大学 | Recombinant stenotrophomonas maltophilia and application thereof in degradation of polycyclic aromatic hydrocarbon wastewater |
| CN112646724B (en) * | 2021-01-11 | 2023-04-21 | 南京工业大学 | Preparation method of composite bacterial liquid preparation |
| CN113773998B (en) * | 2021-10-09 | 2023-03-21 | 广东海洋大学 | PCBs composite degrading microbial inoculum and application thereof |
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