CN104805037B - Achromobacter (Achromobacter sp.) MT H of one plant of degraded diisooctyl phthalate - Google Patents
Achromobacter (Achromobacter sp.) MT H of one plant of degraded diisooctyl phthalate Download PDFInfo
- Publication number
- CN104805037B CN104805037B CN201510066134.8A CN201510066134A CN104805037B CN 104805037 B CN104805037 B CN 104805037B CN 201510066134 A CN201510066134 A CN 201510066134A CN 104805037 B CN104805037 B CN 104805037B
- Authority
- CN
- China
- Prior art keywords
- dehp
- achromobacter
- bacterium
- degraded
- degradation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/025—Achromobacter
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D2101/00—Harmful chemical substances made harmless, or less harmful, by effecting chemical change
- A62D2101/20—Organic substances
- A62D2101/28—Organic substances containing oxygen, sulfur, selenium or tellurium, i.e. chalcogen
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Business, Economics & Management (AREA)
- Emergency Management (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to environment pollutant biological treatment technical field, specifically disclose one plant of degraded diisooctyl phthalate achromobacter (Achromobactersp.)MT‑H.The bacterial strain was deposited in China typical culture collection center on January 22nd, 2015（CCTCC）, deposit number is CCTCC NO：M 2015058.The bacterium has very high degradation efficiency to DEHP, and the DEHP that can preferably be degraded in wider pH and temperature range, it was demonstrated that in terms of MT H can be applied to the biological treatment of DEHP pollutions as excellent DEHP degradation bacterias.
Description
Technical field
The present invention relates to environment pollutant biological treatment technical field, in particular it relates to one plant of new phthalic acid two
Monooctyl ester degradation bacteria, more particularly, to one plant degraded diisooctyl phthalate achromobacter (Achromobacter
sp.)MT-H。
Background technology
Phthalic acid ester, also known as phthalate ester, abridge PAEs, is the general designation for the ester that phthalic acid is formed.Wherein, adjacent benzene
Dioctyl phthalate di-isooctyl（DEHP）It is a kind of typical phthalate material, purposes is very wide in people's daily life
It is general, especially as plastic additive, up to 20~50% in weight.Because DEHP is easily transferred into ring in plastic products
Border, so as to be widely present in air, water body, soil and organism.DEHP in phthalate Substance Intoxication most
By force, can enter by all means in organism, severe jamming its endocrine, reproduction and nervous system, and with extremely strong
Teratogenesis, mutagenicity, carcinogenicity.At present, DEHP has turned into one of most common pollutant in the whole world, and by American National ring
Guarantor office and China Environmental Monitoring General Station are classified as priority pollutants.
DEHP hydrolysis, photodissociation and rate of volatilization are very slow in natural environment, and biodegradation turns into it from environment
The main path of disappearance.But because DEHP side chain is longer, although many DEHP that can degrade strain in soil be present, it is certainly
Right degradation capability is also extremely weak, far can not meet the needs of DEHP environmental pollution improvements.Therefore, the processing to DEHP needs
In seeking efficient degrading bacteria.
At present for microbial degradation DEHP, numerous studies are carried out both at home and abroad.For example, the screening such as Kim is isolated
One plant of Fusarium oxysporum, and one plant of Rhodococcus sp isolating such as Chao and Cheng is respectively provided with good degraded effect to DEHP
Fruit.But the bacterial strain reported is not met by the requirement of actual contamination control, and relevant energy degraded to DEHP degradation rate
DEHP achromobacter germ plasm resource is rarely reported.
The content of the invention
The present invention is in order to overcome the above-mentioned deficiency of prior art, there is provided one plant of new diisooctyl phthalate degraded
Bacterium.
To achieve these goals, the present invention is achieved by following scheme：
One plant of achromobacter (AchromobacterSp.) bacterial strain MT-H, the bacterial strain were deposited on January 22nd, 2015
China typical culture collection center（CCTCC）, deposit number is CCTCC NO:M 2015058, preservation address are：It is Chinese military
Chinese Wuhan University, strain classification are named asAchromobactersp. MT-H。
Achromobacter of the present invention (AchromobacterSp.) bacterial strain MT-H is dirty from Guangzhou great Tan Shachen City city
The returned sludge of water treatment plant, through artificial enrichment culture, isolate and purify to obtain.The bacterial strain there is very high degraded to imitate DEHP
Rate, and the DEHP that can preferably be degraded in wider pH and temperature range, it was demonstrated that MT-H bacterial strains can be as excellent DEHP
In terms of degradation bacteria is applied to the biological treatment of DEHP pollutions.
Achromobacter (AchromobacterSp.) bacterium colony that bacterial strain MT-H is cultivated seven days on LB culture mediums is circle,
Microprotrusion, faint yellow, moistening, translucent, neat in edge are smooth.Under ESEM be mostly spherical or ellipsoid, a diameter of 0.5 ~
1μm。
Achromobacter (AchromobacterSp.) bacterial strain MT-H 16S rDNA gene orders such as SEQ ID NO:1 institute
Show, pass through NCBI official websites（http://www.ncbi.nlm.nih.gov/）Blast program with other logged in bacterium
Bacterial strain 16S rDNA sequences are compared, the results showed that the bacterial strain withAchromobacterSp. similitude highest, homology reach
99%。
Compared with prior art, the invention has the advantages that：
The invention provides new strains-achromobacter of a high-efficiency degradation DEHP (AchromobacterSp.) bacterial strain
MT-H, enrich the germplasm resource bank of phthalic acid ester degradation bacteria, and the bacterium equal energy in wider pH and temperature range
Preferably degrade DEHP, it was demonstrated that MT-H bacterial strains can be applied to the biological treatment side of DEHP pollutions as excellent DEHP degradation bacterias
Face.
Brief description of the drawings
Fig. 1 is the growthform feature that MT-H bacterium are cultivated 7 days on LB culture mediums.
Fig. 2 is the stereoscan photograph of MT-H bacterium.
Fig. 3 is the 16S rDNA of MT-H bacterium phylogenetic tree.
Fig. 4 is degradation effect of the MT-H bacterium to the DEHP of various concentrations.
Fig. 5 be MT-H bacterium at different conditions（PH, temperature and rotating speed）To DEHP degradation effect.
Embodiment
The present invention is further elaborated on reference to Figure of description and specific embodiment.Following examples of the present invention
For the preferable embodiment of the present invention, the present invention mainly illustrates the bacterial strain and the application thought based on the bacterial strain, implements
The replacement of simple parameter can not repeat in embodiment one by one in mode, but therefore not limit the scope of the invention, its
His any Spirit Essence without departing from the present invention with made under principle change, modification, replacement, combine, simplification, should be considered as
The substitute mode of effect, within protection scope of the present invention.
Culture medium prescription is as follows described in embodiment：
Inorganic salts nutrient solution（MSM, g/L）：K2HPO4：5.8, KH2PO4：4.5, (NH4)2SO4：2.0, MgCl2：0.16,
CaCl2：0.02, Na2MoO4·2H2O：0.0024, FeCl3：0.0018, MnCl2·2H2O：0.0015.Final pH is 7.5.
Beef-protein medium（LB）：The g of dusty yeast 5.0, the g of peptone 10.0, the g of sodium chloride 10.0, add ultra-pure water
To 1L, pH=7.0 are adjusted；121 DEG C of sterilizing 20min.Solid plate then adds 1.5%（w/v）Agar powder.
The separation and identification of the bacterial strain of embodiment 1
The returned sludge of Guangzhou great Tan Sha municipal sewage plants is gathered, weighs 5 g activated sludge samples in containing 50
In 150 mL triangular flasks of mL sterilized waters, at 30 DEG C, 140 rpm take 5 mL sludge suspensions to be added to 100 mL to contain after cultivating 3 days
There is DEHP（50 mg L-1）Above-mentioned MSM culture mediums in.Through 30 DEG C, after 140 rpm are cultivated 7 days, 1 mL inoculation is pressed every time
The continuous enrichment of amount, switching 10 times, and the content of DEHP in culture medium is accordingly improved to 1000 mg L-1.Then will transfer 10 times
Nutrient solution dilution 103~105It is coated on again on LB solid plates, 30 DEG C are inverted culture 1 ~ 3 day.After single bacterium colony is grown on flat board,
Picking single bacterium colony is repeatedly rule purifying, and separation obtains one plant of bacterium, numbering MT-H.Then by inoculation on LB solid plates
30 DEG C are inverted culture 7 days, observe its colonial morphology.For the bacterium colony of LB flat boards culture 7 days into circle, microprotrusion is faint yellow, moistening,
Translucent, neat in edge is smooth（See Fig. 1）.
Scanning electron microscope example prepares and observation：Bacterial strain MT-H after purification is accessed into the LB fluid nutrient mediums containing 10 mL
It is activated overnight.Draw 800 μ L bacterium solutions and centrifuge 3 ~ 5 min through 8000 rpm, remove supernatant, add 500 μ L PBS and wash bacterium 3 times.
2.5% glutaraldehyde that 1 mL is added in the bacterial sediment of harvest fully mixes, and 4 DEG C stand overnight.Then again through 8000 rpm
3 ~ 5 min are centrifuged, remove supernatant, 500 μ L PBS is added and washes bacterium 3 times.Then by thalline respectively 30%, 50%, 70%, 85%,
It is dehydrated 2 times in 90% and 100% graded ethanol, each gradient about soaks 15 min, and supernatant is removed in then 8000 rpm centrifugations, most
Ethanol is replaced with isoamyl acetate 2 times, every time 20 min, the same ethanol dehydration of method afterwards.By CO2Film-making is observed after drying.Sweep
Retouch under Electronic Speculum it is observed that the bacterium is in spherical or ellipsoid, diameter is about 0.5 ~ 1 μm（See Fig. 2）.
The 16S rDNA Molecular Identifications of bacterial strain：Bacteria total DNA is extracted, with bacterial 16 S rDNA universal primers to the bacterium gene
Group enters performing PCR amplification.PCR primer is through sequencing（Sequencing is completed by Shanghai life work）Afterwards, sequence such as SEQ ID NO：Shown in 1, it will survey
The 16S rDNA sequences reported in sequence result and GenBank carry out sequence analysis, and if choosing dry strain and doing chadogram point
Analysis.As shown in figure 3, the present invention isolates and purifies obtained bacterial strain MT-H 16S rDNA sequences and achromobacter
（Achromobactersp. D-12）For homology up to 99%, evolutionary distance is nearest.Its cultural characteristic and scanning electron microscopic observation feature
With achromobacter（Achromobactersp.）Also it is the most similar, therefore the degradation bacteria that present invention screening obtains is achromobacter
（Achromobactersp.）.
Therefore, the bacterial strain was deposited in China typical culture collection center by inventor on January 22nd, 2015
（CCTCC）, deposit number is CCTCC NO:M 2015058, preservation address are：Wuhan, China Wuhan University, strain classification life
It is entitledAchromobactersp. MT-H。
Embodiment 2AchromobacterSp.MT-H tests to DEHP degradation effect
S1. prepared by bacteria suspension：LB fluid nutrient medium of the strain MT-H accesses containing 10 mL after purification is activated overnight training
Support to logarithmic phase, centrifuging 10 min through 5000 rpm collects thalline, is resuspended after washing bacterium 3 times with PBS, adjusts OD600 nm = 1.0
As bacteria suspension.
S2. degradation property determines：Contain various concentrations DEHP to 100mL respectively（50、100、200、400、600 mg L-1）
MSM nutrient solutions in meet the mL of bacterium 1, not connect bacterium as control, and adjust pH as 8.0, every group of three repetitions.At 30 DEG C, 140
Rpm constant-temperature tables culture 9 days, periodically sampling, the degraded situation through GC/MS measure DEHP.
Chromatographic condition：Using Shimadzu Corporation's QP2010 Plus type GC/MS tandem mass spectrometers.Chromatographic column is Agilent HP-5
Pillar (0.25 μm × 0.25 mm × 30 m), injector temperature are 250 DEG C, ion gun（EI）Temperature is 220 DEG C, using not
The μ L of split sampling 1, carrier gas is high-purity helium.Heating schedule is：Initial temperature is 100 DEG C, keeps 2 min, 15 DEG C/min ladders
Degree rises to 129 DEG C, is then warming up to 280 DEG C with 40 DEG C/min, keeps 5min.
Quality control：Standard curve is made using external standard method and six point calibration standard substances.6 kinds of PAEs mix target matrix and added
It is 92.5 ~ 110.2% to mark average recovery rate, and relative deviation is less than 10.5%, and instrument detection is limited to 0.12-0.45 ug g-1.The party
Method meets trace organic substance quantitative analysis requirement.
As a result as shown in figure 4,AchromobacterSp. MT-H is very slow to DEHP degradation rate in Initial stage of culture
Slowly, as the extension of incubation time, its degradation also increase therewith；It is less than 200 mg L in DEHP concentration-1When, DEHP in 7d
It is almost degradable；When DEHP concentration is higher, though its degradation rate is substantially suppressed；When DEHP concentration up to 600
mg L-1When, cultivate to the 9th day, its degradation rate is also up to more than 50%.
This example demonstrates that resulting separationAchromobacterSp. MT-H can by the use of DEHP as sole carbon source and
The energy carries out growth and breeding, and when DEHP concentration is relatively low, the bacterium has efficient degradation DEHP ability.
The pH of embodiment 3, temperature and rotating speed pairAchromobacterSp. MT-H degradeds DEHP influence
PH pairsAchromobacterSp.MT-H degradeds DEHP influence：Respectively to 100mL differences pH (5,6,7,8,9,
10) contain DEHP（500mg/L）MSM nutrient solutions in meet bacterium 1mL, using do not connect bacterium as control, every group of three repetitions.30
DEG C, after 140 rpm constant-temperature table cultures 48h, DEHP degraded situation is determined through GC/MS.
Temperature pairAchromobacterSp.MT-H degradeds DEHP influence：Contain DEHP to 100mL（500mg/L）'s
Bacterium 2.5mL is met in MSM nutrient solutions, using do not connect bacterium as control, every group of three repetitions, respectively temperature be 20 DEG C, 25 DEG C, 30
DEG C, 35 DEG C, cultivate 48h through 140 rpm in 40 DEG C of constant-temperature table after, DEHP degraded situation is determined through GC/MS.
Rotating speed pairAchromobacterSp. MT-H degradeds DEHP influence：Contain DEHP to 100mL（500mg/L）'s
Bacterium 2.5mL is met in MSM nutrient solutions, using do not connect bacterium as control, every group of three repetitions, respectively rotating speed be 80,110,140,
170th, the constant-temperature table of 200 rpm rotating speeds determines DEHP degraded situation through GC/MS after 30 DEG C of culture 48h.
Experimental result as shown in figure 5, fromAchromobacterSp. degraded of the MT-H bacterium under condition of different pH to DEHP
Design sketch can be seen thatAchromobacterSp. MT-H bacterium are different to DEHP degradation capability under condition of different pH.In pH
In the range of 6 ~ 9, the bacterium reaches more than 80% to DEHP degradation rate；Especially pH is that degradation efficiency can in the range of 7-8
Up to more than 90%；When pH is 5, degradation rate is suppressed by larger.FromAchromobacterSp. MT-H bacterium are in different temperatures bar
DEHP degradation effect figure can be seen that when temperature is in the range of 30 ~ 40 DEG C under part, the bacterium is reachable to DEHP degradation rate
More than 80%；But at a lower temperature, its degradation rate is substantially inhibited.FromAchromobacterSp. MT-H bacterium are in difference
Can be seen that rotating speed under speed conditions on DEHP degradation effect figure influences not being very aobvious on the DEHP degradation efficiencies of the bacterium
Write, approximate trend is that rotating speed is more high more be advantageous to the bacterium and degrade DEHP, but when rotating speed reaches 170rpm, its degradation rate becomes substantially
In constant.
This example demonstrates that bacterial strainAchromobacterSp. MT-H is in neutral and meta-alkalescence wider pH and temperature model
The interior DEHP that can preferably degrade is enclosed, guarantee is provided for its application in different complex environments.
SEQUENCE LISTING
<110>Ji'nan University
<120>Achromobacter (Achromobacter sp.) MT-H of one plant of degraded diisooctyl phthalate
<130>
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 1419
<212> DNA
<213>The 16S rDNA sequences of MT-H bacterial strains
<400> 1
tgagacgatc taccgtggta tcgccctcct tgcggttagt gctaactact tctggtaaaa 60
cccactccca tggtgtgacg ggcggtgtgt acaagacccg ggaacgtatt caccgcgaca 120
tgctgatccg cgattactag cgattccgac ttcacgcagt cgagttgcag actgcgatcc 180
ggactacgat cgggtttctg ggattggctc cccctcgcgg gttggcgacc ctctgtcccg 240
accattgtat gacgtgtgaa gccctaccca taagggccat gaggacttga cgtcatcccc 300
accttcctcc ggtttgtcac cggcagtctc attagagtgc cctttcgtag caactaatga 360
caagggttgc gctcgttgcg ggacttaacc caacatctca cgacacgagc tgacgacagc 420
catgcagcac ctgtgttccg gttctcttgc gagcactccc aaatctcttc rggattccag 480
acatgtcaag ggtaggtaag gtttttcgcg ttgcatcgaa ttaatccaca tcatccaccg 540
cttgtgcggg tccccgtcaa ttcctttgag ttttaatctt gcgaccgtac tccccaggcg 600
gtcaacttca cgcgttagct gcgctaccaa ggtccgaaga ccccaacagc tagttgacat 660
cgtttagggc gtggactacc agggtatcta atcctgtttg ctccccacgc tttcgtgcat 720
gagcgtcagt gttatcccag gaggctgcct tcgccatcgg tgttcctccg catatctacg 780
catttcactg ctacacgcgg aattccacct ccctctgaca cactctagcc cggtagttaa 840
aaatgcagtt ccaaagttaa gctctgggat ttcacatctt tctttccgaa ccgcctgcgc 900
acgctttacg cccagtaatt ccgattaacg cttgcaccct acgtattacc gcggctgctg 960
gcacgtagtt agccggtgct tattctgcag gtaccgtcag ttccgcgggg tattaacccg 1020
cgacgtttct ttcctgccaa aagtgcttta caacccgaag gccttcatcg cacacgcggg 1080
atggctggat cagggtttcc cccattgtcc aaaattcccc actgctgcct cccgtaggag 1140
tctgggccgt gtctcagtcc cagtgtggct ggtcgtcctc tcaaaccagc tacggatcgt 1200
cgccttggtg agccgttacc ccaccaacta gctaatccga tatcggccgc tccaatagtg 1260
caaggtcttg cgatcccctg ctttcccccg tagggcgtat gcggtattag ctacgctttc 1320
gcgtagttat cccccgctac tgggcacgtt ccgatacatt actcacccgt tcgccactcg 1380
ccaccagacc gaagttgcgt gctgccgttc gactgcatg 1419
Claims (1)
1. one plant of achromobacter (AchromobacterSp.) bacterial strain MT-H, the bacterial strain is on January 22nd, 2015 is deposited in
State's Type Tissue Collection（CCTCC）, deposit number is CCTCC NO: M 2015058.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510066134.8A CN104805037B (en) | 2015-02-09 | 2015-02-09 | Achromobacter (Achromobacter sp.) MT H of one plant of degraded diisooctyl phthalate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510066134.8A CN104805037B (en) | 2015-02-09 | 2015-02-09 | Achromobacter (Achromobacter sp.) MT H of one plant of degraded diisooctyl phthalate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104805037A CN104805037A (en) | 2015-07-29 |
| CN104805037B true CN104805037B (en) | 2017-12-29 |
Family
ID=53690240
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510066134.8A Active CN104805037B (en) | 2015-02-09 | 2015-02-09 | Achromobacter (Achromobacter sp.) MT H of one plant of degraded diisooctyl phthalate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104805037B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107557315B (en) * | 2017-09-12 | 2020-08-04 | 中国农业科学院农业资源与农业区划研究所 | Tylosin degrading bacterium and application thereof |
| CN113717878B (en) * | 2021-07-09 | 2023-08-18 | 上海圣珑环境科技有限公司 | Ethylbenzene degrading bacterium and screening method and application thereof |
-
2015
- 2015-02-09 CN CN201510066134.8A patent/CN104805037B/en active Active
Non-Patent Citations (3)
| Title |
|---|
| Achromobacter denitrificans strain SP1efficiently remediates di(2-ethylhexyl)phthalate;Pradeep S等;《Ecotoxicol Environ Saf》;20141112;摘要 * |
| Occurrence fate and effects of Di(2-ethylhexyl)phthalate in wastewater treatment plants:a review;Zolfaghari M等;《Environmental pollution》;20141231(第194期);281-293 * |
| 环境内分泌干扰物：邻苯二甲酸酯的生物降解研究进展;络祝华等;《应用与环境生物学报》;20081231;第14卷(第6期);890-897 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104805037A (en) | 2015-07-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Talha et al. | Bioremediation of Congo red dye in immobilized batch and continuous packed bed bioreactor by Brevibacillus parabrevis using coconut shell bio-char | |
| Gupta et al. | Isolation and characterization of heavy metal tolerant Gram-positive bacteria with bioremedial properties from municipal waste rich soil of Kestopur canal (Kolkata), West Bengal, India | |
| CN107287134B (en) | The preparation method and application of one pseudomonas and its bifunctional enzyme preparation | |
| CN106434470B (en) | A kind of polycyclic aromatic hydrocarbon-degrading bacteria and its application | |
| CN106497810B (en) | A kind of method of germ oligotrophy unit cell, the microbial inoculum containing the bacterium and its application and diesel oil of degrading | |
| CN104805033B (en) | The microbacterium of one plant of degradable a variety of phthalic acid ester（Microbacterium sp.）J-1 | |
| CN106916765B (en) | A method of heavy metal in waste water zinc is adsorbed using penicillium janthinellum | |
| CN104403965B (en) | A kind of roost rose of Sharon pseudomonad of water body tetracycline pollutant of degrading and its application | |
| CN114107092A (en) | Plant endophyte Gordonia L191 for degrading phthalate and application thereof | |
| CN110283741A (en) | One plant of rose bacillus and its application with efficient degradation polycyclic aromatic hydrocarbon function | |
| CN104805036A (en) | Applications of Microbacterium sp. J-1 in degradation of plurality of phthalic acid esters | |
| Rajendran et al. | Degradation of heavy metal contaminated soil using plant growth promoting rhizobacteria (PGPR): Assess their remediation potential and growth influence of Vigna radiata. L. | |
| CN104845899A (en) | Application of Rhodococcus sp. 2G in degradation of phthalate | |
| CN104805035A (en) | Rhodococcus sp. 2G used for simultaneous degradation of plurality of phthalic acid esters | |
| CN107893047A (en) | One plant of oil fragrance surfactant hydrocarbon degradation bacteria and its application | |
| CN110846254A (en) | Compound microbial agent for denitrification and preparation method and application thereof | |
| CN111004736A (en) | Bacillus megaterium and application thereof in degrading pyrethroid insecticides | |
| CN104805037B (en) | Achromobacter (Achromobacter sp.) MT H of one plant of degraded diisooctyl phthalate | |
| CN104845902B (en) | Applications of achromobacter (Achromobacter sp.) the MT H in diisooctyl phthalate of degrading | |
| CN104805018B (en) | Earth mould (Agromyces sp.) MT E of one plant of a variety of phthalic acid ester that can degrade simultaneously | |
| CN104845898B (en) | Providence (Providencia sp.) 2D of one high-efficiency degradation dibutyl phthalate | |
| CN106434413B (en) | One kind is planted raw Raoul bacterium and the method using pyrene in bacterium degradation soil | |
| CN104845890B (en) | Applications of earth mould (Agromyces sp.) the MT E in a variety of phthalic acid esters of degrading | |
| CN105316267B (en) | A kind of total eclipse pair meningitidis strains and its application | |
| CN104745515B (en) | A kind of acinetobacter calcoaceticus of degrading polycyclic aromatic hydrocarbons and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |