CN106496345A - A kind of extracting method of algal polysaccharides - Google Patents
A kind of extracting method of algal polysaccharides Download PDFInfo
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- CN106496345A CN106496345A CN201611037239.1A CN201611037239A CN106496345A CN 106496345 A CN106496345 A CN 106496345A CN 201611037239 A CN201611037239 A CN 201611037239A CN 106496345 A CN106496345 A CN 106496345A
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- China
- Prior art keywords
- algal polysaccharides
- extracting method
- irradiation
- seawood meal
- algal
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention discloses a kind of extracting method of algal polysaccharides, the extracting method includes：Seawood meal is positioned in radiation field carries out irradiation；During the present invention extracts algal polysaccharides, seawood meal is extracted after irradiation, and the recovery rate for making algal polysaccharides is original 24 times, significantly improves the recovery rate of algal polysaccharides, and the algal polysaccharides activity that extracts is not affected；Extract through high-pressure pulse electric in preferred scheme, the DNA purity of algal polysaccharides can be improved, soaked in absolute ethyl alcohol seawood meal is adopted before extraction, using algal polysaccharides not ethanol, soaked by ethanol and remove the following impurity that can be dissolved in ethanol, play a part of algal polysaccharides purification；Irradiation is carried out again after freezing, the efficiency of irradiation can be improved；Totality of the invention has the beneficial effect that in sum, improves recovery rate, the purity of algal polysaccharides of algal polysaccharides, keeps algal polysaccharides activity.
Description
Technical field
The present invention relates to technical field of polysaccharide extraction, and in particular to a kind of extracting method of algal polysaccharides.
Background technology
Algal polysaccharides are essentially from marine algas such as sea-tangle, deer's tail dish (sargassum fusifome), bulk kelp, yellow tang, bladder-wracks.Algal polysaccharides
Mainly include algin, fucoidin and laminaran.Isolated algin, fucoidin and laminaran from sea-tangle
Crude product is the powder of white yellowish.The purified sodium alginate for obtaining is white filiform；Fucoidin is milky
Powder；The two is dissolved in water, does not dissolve in the organic solvents such as ethanol, acetone, chloroform.The method that current algal polysaccharides are extracted is main
There is water extract-alcohol precipitation to extract, ultrasonic wave extraction, Microwave Extraction, enzyme extraction etc., wherein water extract-alcohol precipitation extraction are also divided into hot water alcohol precipitation and carry
Take, cold water alcohol precipitation is extracted, acidleach formulation and alkali method, hot water water alcohol precipitation extracts the recovery rate extracted relative to cold water alcohol precipitation
Height, but the research such as Wang Dongfeng confirms that the heat endurance of algal polysaccharides is poor；Acidleach formulation and alkali method are easily destroyed polysaccharide
Structure, causes algal polysaccharides to lose；Ultrasonic wave extraction can cause the activity of algal polysaccharides to reduce or inactivate；Microwave Extraction high energy consumption
And temperature is high；Enzyme extraction method causes extraction cost too high due to enzyme price height；The recovery rate of existing algal polysaccharides a maximum of about of 5%.
Content of the invention
A kind of simple and convenient, recovery rate of one object of the present invention offer is high, the active marine alga not reduced of algal polysaccharides is more
The extracting method of sugar.
The present invention provide technical scheme be：
A kind of extracting method of algal polysaccharides, the extracting method include：Seawood meal is positioned in radiation field carries out spoke
According to.
Preferably, the extracting method specifically includes following steps：1) marine alga is carried out after liquid nitrogen gas crushing, mistake
Seawood meal is obtained after 60 mesh sieve, seawood meal is adopted soaked in absolute ethyl alcohol 0.5-1 hour；2) decompression recycling ethanol, by marine alga
After subzero 20-30 DEG C freezing 1 hour, normally rise again to be positioned in radiation field to 30 DEG C carries out irradiation to powder；3) will be through irradiation
Seawood meal and water carried out mixing and be placed in high-pressure pulse electric extraction in 1g: 10-20ml ratio, filter be evaporated to original
Volume 25%, adds ethanol precipitation to extract sediment, and freeze-drying sediment obtains the algal polysaccharides.
Preferably, seawood meal is positioned in radiation field carries out 2-20 kilocurie dose deliveries, this irradiation dose energy
Significantly reduce in extraction process to algal polysaccharides activity influence.
Preferably, the time of the irradiation be 10-20 minutes, this time algal polysaccharides recovery rate highest, and when
Between short can reduce irradiation cost.
Preferably, the high-pressure pulse electric, wherein electric-field intensity are 30-50kv, and umber of pulse is 20.
Preferably, the time that the high-pressure pulse electric is extracted is 20-30 minutes.
Beneficial effects of the present invention are as follows：
During the present invention extracts algal polysaccharides, seawood meal is extracted after irradiation, and the recovery rate for making algal polysaccharides is
2-4 times originally, significantly improves the recovery rate of algal polysaccharides, and the algal polysaccharides activity that extracts is not affected；Preferred scheme
Middle through high-pressure pulse electric extract, the DNA purity of algal polysaccharides can be improved, before extraction adopt soaked in absolute ethyl alcohol marine alga
Powder, using algal polysaccharides not ethanol, is soaked by ethanol and removes the following impurity that can be dissolved in ethanol, play algal polysaccharides purification
Effect；Irradiation is carried out again after freezing, the efficiency of irradiation can be improved；Totality of the invention has the beneficial effect that in sum, carries
The recovery rate of high algal polysaccharides, the purity of algal polysaccharides, keep algal polysaccharides activity.
Specific embodiment
With reference to specific embodiment, the present invention is described in further detail, to make those skilled in the art's reference say
Bright book word can be implemented according to this.
Using Fujian Anxi oolong marine alga as embodiment extraction sample.
Embodiment 1
A kind of extracting method of algal polysaccharides, the extracting method specifically include following steps：1) marine alga is carried out liquid
After nitrogen is crushed, after crossing 60 mesh sieve, seawood meal is obtained, seawood meal is adopted soaked in absolute ethyl alcohol 0.5 hour, absolute ethyl alcohol
Weight ratio preferably 5: 1 with seawood meal；2) decompression recycling ethanol, by seawood meal after subzero 30 DEG C freezings 1 hour, normally rises again
Being positioned in radiation field to 30 DEG C carries out 20 kilocurie dose deliveries, and the time of irradiation is 20 minutes；3) by through the sea of irradiation
Algae powder and water are carried out mixing and are placed in high-pressure pulse electric extraction in 1g: 20ml ratio, and wherein electric-field intensity is 50kv, umber of pulse
For 20, the time that high-pressure pulse electric is extracted is 30 minutes, is filtrated to get liquid, liquid is put in Rotary Evaporators and is subtracted
Pressure is concentrated into original liquid volume 25%, adds 5 times of weight absolute ethyl alcohol precipitations, extracts sediment after centrifugation, and freeze-drying is precipitated
Thing obtains the algal polysaccharides.
Embodiment 2
A kind of extracting method of algal polysaccharides, the extracting method specifically include following steps：1) marine alga is carried out liquid
After nitrogen is crushed, after crossing 60 mesh sieve, obtain seawood meal, by seawood meal using soaked in absolute ethyl alcohol 1 hour, absolute ethyl alcohol with
The weight ratio preferably 5: 1 of seawood meal；2) decompression recycling ethanol, by seawood meal subzero 20 DEG C freezing 1 hour after, normally rise again to
30 DEG C are positioned in radiation field and carry out 2 kilocurie dose deliveries, and the time of irradiation is 10 minutes；3) by the seawood meal through irradiation
Carried out mixing and be placed in high-pressure pulse electric extraction in 1g: 10ml ratio with water, wherein electric-field intensity is 30ky, and umber of pulse is
20, the time that high-pressure pulse electric is extracted is 20 minutes, is filtrated to get liquid, liquid is put in Rotary Evaporators and is reduced pressure
Original liquid volume 25% is concentrated into, 10 times of weight absolute ethyl alcohol precipitations is added, after centrifugation, is extracted sediment, freeze-drying sediment
Obtain the algal polysaccharides.
Embodiment 3
A kind of extracting method of algal polysaccharides, the extracting method specifically include following steps：1) marine alga is carried out liquid
After nitrogen is crushed, after crossing 60 mesh sieve, obtain seawood meal, by seawood meal using soaked in absolute ethyl alcohol 1 hour, absolute ethyl alcohol with
The weight ratio preferably 5: 1 of seawood meal；2) decompression recycling ethanol, by seawood meal subzero 20 DEG C freezing 1 hour after, normally rise again to
30 DEG C are positioned in radiation field and carry out 25 kilocurie dose deliveries, and the time of irradiation is 30 minutes；3) by the marine alga through irradiation
Powder and water are carried out mixing and are placed in high-pressure pulse electric extraction in 1g: 10ml ratio, and wherein electric-field intensity is 30ky, and umber of pulse is
20, the time that high-pressure pulse electric is extracted is 20 minutes, is filtrated to get liquid, liquid is put in Rotary Evaporators and is reduced pressure
Original liquid volume 25% is concentrated into, 10 times of weight absolute ethyl alcohol precipitations is added, after centrifugation, is extracted sediment, freeze-drying sediment
Obtain the algal polysaccharides.
Comparative example 1
Same as Example 1, lack irradiation process.
Comparative example 2
Same as Example 1, lack the process that irradiation process and seawood meal are soaked through ethanol.
Comparative example 3
Same as Example 1, lack irradiation process, high-pressure pulse electric is extracted and is substituted for ultrasonic wave extraction.
Polyoses content is tested using spectrophotometer, algal polysaccharides recovery rate and algal polysaccharides purity is calculated, as a result as follows
Table：
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 | |
| Recovery rate (%) | 17.6 | 16.5 | 11.2 | 8.3 | 6.4 | 7.3 |
| Purity (%) | 94.2 | 93.3 | 91.6 | 82.1 | 80.7 | 79.6 |
Although embodiment of the present invention is disclosed as above, which is not restricted in specification and embodiment listed
With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily
Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited
In specific details and shown here as the embodiment with description.
Claims (6)
1. a kind of extracting method of algal polysaccharides, it is characterised in that the extracting method includes：Seawood meal is positioned over radiation field
In carry out irradiation.
2. the extracting method of algal polysaccharides as claimed in claim 1, it is characterised in that the extracting method specifically include with
Lower step：1) marine alga is carried out after liquid nitrogen gas crushing, seawood meal is obtained after crossing 60 mesh sieve, seawood meal is adopted absolute ethyl alcohol
Immersion 0.5-1 hours；2) decompression recycling ethanol, by seawood meal after subzero 20-30 DEG C freezing 1 hour, normally rises again to 30 DEG C
Being positioned in radiation field carries out irradiation；3) seawood meal through irradiation and water are carried out mixing and are placed in 1g: 10-20ml ratio
High-pressure pulse electric is extracted, and is filtered and is evaporated to original volume 25%, adds ethanol precipitation to extract sediment, and freeze-drying is sunk
Starch obtains the algal polysaccharides.
3. the extracting method of algal polysaccharides as claimed in claim 1 or 2, it is characterised in that seawood meal is positioned over radiation field
In carry out 2-20 kilocurie dose deliveries.
4. the extracting method of algal polysaccharides as claimed in claim 1 or 2, it is characterised in that the time of the irradiation is 10-20
Minute.
5. the extracting method of algal polysaccharides as claimed in claim 2, it is characterised in that the high-pressure pulse electric, wherein electricity
Field intensity is 30-50kv, and umber of pulse is 20.
6. the extracting method of algal polysaccharides as claimed in claim 2, it is characterised in that the high-pressure pulse electric extract when
Between be 20-30 minutes.
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| CN201611037239.1A CN106496345A (en) | 2016-11-22 | 2016-11-22 | A kind of extracting method of algal polysaccharides |
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| CN201611037239.1A CN106496345A (en) | 2016-11-22 | 2016-11-22 | A kind of extracting method of algal polysaccharides |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110693772A (en) * | 2019-11-12 | 2020-01-17 | 烟台新时代健康产业日化有限公司 | Preparation method of seaweed anti-inflammatory extract applied to cosmetics |
| CN112375154A (en) * | 2020-11-02 | 2021-02-19 | 江苏泰德医药有限公司 | Method for extracting fucoidan |
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Application publication date: 20170315 |