CN106490625B - A kind of application of rice active peptide in preparation protection endothelial progenitor cells anti-oxidation preparation - Google Patents
A kind of application of rice active peptide in preparation protection endothelial progenitor cells anti-oxidation preparation Download PDFInfo
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- CN106490625B CN106490625B CN201610932383.5A CN201610932383A CN106490625B CN 106490625 B CN106490625 B CN 106490625B CN 201610932383 A CN201610932383 A CN 201610932383A CN 106490625 B CN106490625 B CN 106490625B
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Abstract
The invention discloses a kind of application of rice active peptide in preparation protection endothelial progenitor cells anti-oxidation preparation or food.Rice active peptide of the present invention has the protection oxidation resistant effect of endothelial progenitor cells, to protect the related disease of endothelial progenitor cells to provide new food-borne application.
Description
Technical field
A kind of application of the rice active peptide of the present invention in preparation protection endothelial progenitor cells anti-oxidation preparation or food.
Background technique
Rice protein has excellent nutritive value, and absorption of human body utilization rate is high, and biological value is big, and rice protein is rich
Containing each amino acid, still, rice protein dissolubility itself is poor, has seriously affected its utilization.Therefore deep processing is carried out to rice,
It is of great significance with further promoting its value.And rice protein is hydrolyzed, it extracts therein biologically active
Peptide fragment can effectively realize the deep processing to rice protein.Rice active peptide is that the one kind extracted from rice has biology
Active peptide.Rice active peptide includes several various active peptides with different function, there is rice antioxidation active peptides, rice drop
Blood pressure active peptide, rice immune-active peptides, opioid peptide, opioids antagonistic peptide, rice flavor peptides etc..The anti-oxidant work of rice
Property peptide can effectively clear the active oxygen of internal surplus, protect the normal configuration and function of cell and mitochondria, prevent lipid peroxy
Change, has very big potentiality to be exploited in terms of the cardiovascular and cerebrovascular disease that prevention free radical induces.Rice active peptide Human Umbilical Vein Endothelial Cells
Oxidative damage has certain protective effect, can effectively remove some free radicals, reduce the apoptosis of endothelial cell.
Endothelial progenitor cells (endothelial progenitor cells, EPCs) are to be present in marrow, bleeding of the umbilicus and periphery
A kind of precursor with high proliferation potential of blood, it, which has, is proliferated, migrates, being divided into mature endothelial cell and aggregation formation
The effect of new vessels.Studies have shown that the reduction of endothelial progenitor cells quantity may be the marker of cardiovascular disease.Endothelial progenitor cells
Because damage caused by oxidative stress is the early reaction of a variety of diseases, with hypertension, coronary heart disease, diabetes, disorders of lipid metabolism
Occur closely related, that there are endothelial progenitor cells functions is impaired by the patients of these diseases.The damage and reparation that endothelial progenitor cells mediate
Reaction is the initiating agent and important link of atherosclerosis.Existing research discovery, some food source substances, integration of drinking and medicinal herbs substance
It can be used for treating disease caused by endothelial progenitor cells damage.Honey may be by promoting the mobilization of endothelial progenitor cells, and then participates in
The generation of new vessels can be used for treating Tissue of Diabetic Wound.Lycopene can by adjust endothelial progenitor cells cell cycle come
Increase cell viability.Endothelial progenitor cells can be enhanced to H in catechin2O2Resistance, reduce its apoptosis.Puerarin can significantly increase
Add the quantity of endothelial progenitor cells, and improves adhesive capacity, transfer ability, proliferative capacity and the external blood of endothelial progenitor cells of peripheral blood
Pipe generative capacity.
By investigation, has no that Chinese scholar has rice active peptide and endothelial progenitor cells is protected to make from oxidativestress damage
Pertinent literature report, therefore the research fields such as activity about rice active peptide anti-oxidative damage are still in blank stage.
Summary of the invention
The present invention establishes hydrogen peroxide induced injury model, finds after administration, rice active peptide (sequence be KHNRGDEF <
SEQ ID NO.1 >) the concentration in 200 μm of ol/L under to H2O2The cell viability improvement of induction is the most obvious.Therefore, institute
Stating rice active peptide can be used for preventing and/or alleviating atherosclerosis relevant to endothelial progenitor cells dysfunction, high blood
Pressure, diabetes etc. due to endogenous or exogeneous reactivity substance accumulation and a series of diseases for inducing.That is, of the present invention big
Rice active peptide has the protection oxidation resistant effect of endothelial progenitor cells, to protect the related disease of endothelial progenitor cells to provide new food
The application of source property.
Specific embodiment
Rice active peptide (SEQ ID NO.1) is to H2O2The anti-oxidation protection of the endothelial progenitor cells of induction acts on
1. selecting the cell of logarithmic growth phase, cell suspension is made after no EDTA pancreatin digestion, measures suspension with tally
In cell concentration, according to 5000, every hole cell inoculation in 96 orifice plates, every hole is inoculated with 200 μ L, and every group sets 6 multiple holes, 37
DEG C 5%CO2It is divided into normal group, hydrogen peroxide treatment group, hydrogen peroxide+rice active peptide protection after cultivating 4 hours in incubator
Original culture medium is sucked out group, all groups of culture mediums for being first added 160 μ L, normal group, hydrogen peroxide treatment group be added 20 μ L's
Serum free medium, hydrogen peroxide+rice active peptide protection group are separately added into the isometric rice active peptide of respective concentration, make drug
Final concentration is respectively 100,150,200 μm of ol/L, in 37 DEG C of 5%CO2After incubator protects 4h in advance, hydrogen peroxide treatment group and mistake
20 μ L H are added in the every hole of hydrogen oxide+rice active peptide protection group2O2, final concentration of 100 μm of l/L, 20 μ L of Normal group addition
Serum free medium, in 37 DEG C of 5%CO2After incubator is incubated for for 24 hours, mtt assay detects cell viability.It is shown in Table 1:
1 rice active peptide of table is to H2O2The influence of the cell viability of the endothelial progenitor cells of damage
Compared with normal group, hydrogen peroxide treatment damage group cell viability is remarkably decreased；And with hydrogen peroxide treatment group ratio
Compared with the high dose group (200 μm of ol/L) of rice active peptide is to H2O2The cell viability of induction has clear improvement.
2. selecting the cell of logarithmic growth phase, cell suspension is made after the pancreatin digestion of no EDTA, adjusts cell concentration, presses
Every hole 4 × 104A to be inoculated in 24 orifice plates, every hole is inoculated with 800 μ L, and every group sets 3 multiple holes, be divided into after 4h normal group, model group and
100 μ L serum free mediums are added in rice active peptide group, normal group and hydrogen peroxide treatment group, and three administration groups are separately added into phase
The isometric rice active peptide of concentration is answered, making drug final concentration is respectively 100,150,200 μm of ol/L, and pre- protection 4h is incubated in 37 DEG C
Afterwards, in addition to 100 μ L serum free mediums are added in normal group, 100 μ L H are added in model group and the every hole of rice active peptide group2O2, end
Concentration is 100 μm of ol/L, and 100 μ L serum free mediums are added in Normal group, and after 37 DEG C of incubation 4h, by specification will be sought
The burst size of cell supernatant measurement LDH and MDA；Lytic cell measures SOD vigor intracellular.Each group is calculated according to formula
The biochemical indicator of cell, is shown in Table 2:
2 rice active peptide of table is to H2O2The influence of the endothelial progenitor cells damage oxidation index of induction
Compared with normal group, hydrogen peroxide treatment group SOD activity is decreased obviously, and LDH and MDA are horizontal significantly raised；And with
Model group compares, and the middle and high dosage group LDH burst size of rice active peptide is remarkably decreased；And in MDA level, rice active peptide is given
Medicine group can be allowed to reduce；In SOD activity, rice active peptide high dose group can be allowed to significantly increase, and show rice active peptide
Have the function of protecting cellular anti-oxidant damage in cellular oxidation index.
3. measuring the cell cycle stage of different disposal group endothelial progenitor cells by flow cytometer, rice active peptide is found
Cell viability can be increased by dramatically increasing the S phase of cell.
Present invention combination most preferred embodiment is described, however after having read above content of the invention, ability
Field technique personnel can appreciate the fact that make in the embodiment disclosed it is many change also can get it is same or similar as a result, and without departing from this
The design of invention, spirit and scope.More specifically, it is clear that some chemistry and the alternative this paper institute of the related reagent of physiological are public
The reagent opened and obtain same or similar result.All similar substitutions and modification it is evident to the person skilled in the art that
It is regarded as in spirit of the invention, scope and spirit and scope of the claims, i.e., all above-mentioned such equivalent forms and institute
There are the improvement to technological parameter and variation all to also belong to claims of the present invention limited range.
Claims (1)
1. a kind of application of rice active peptide in preparation protection endothelial progenitor cells anti-oxidation preparation or food, the rice are living
The sequence of property peptide is as shown in SEQ ID NO.1.
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| CN110029139A (en) * | 2019-01-31 | 2019-07-19 | 山东大树达孚特膳食品有限公司 | A kind of production technology of rice biologically active peptide |
| CN113616773B (en) * | 2021-08-25 | 2023-11-14 | 中南林业科技大学 | Application of rice bran active peptide in intervention of caenorhabditis elegans aging or muscle injury |
| CN114558106B (en) * | 2022-03-23 | 2024-02-23 | 长沙飞创生物技术有限责任公司 | Application of rice bran active peptide in preventing or treating lipotoxicity related diseases |
| CN114478702B (en) * | 2022-04-06 | 2023-12-12 | 中南林业科技大学 | Rice bran-derived short peptide and application thereof in treating skin injury |
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Non-Patent Citations (3)
| Title |
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| 大米蛋白体外消化产物抗氧化活性的研究;佟立涛等;《现代食品科技》;20151231;第31卷(第9期);第92-97页 * |
| 大米蛋白抗氧化肽的制备、分离纯化和结构鉴定;张君慧;《中国博士学位论文全文数据库（电子期刊）工程科技I辑》;20101215(第12期);摘要、第3.3-3.5节、第4.3-4.5节及第5.3-5.5节 * |
| 载脂蛋白A-I模拟肽L- 4F减轻过氧化氢诱导的小鼠骨髓内皮祖细胞损伤;展恩欣等;《中国病理生理杂志》;20160517;第32卷(第1期);摘要，第2页左栏第1段至第6页右栏第3段 * |
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Effective date of registration: 20230423 Address after: Room 609-B, 6th Floor, Building 007, Phase II Plant, No. 8 Lutian Road, Lugu Base, Changsha High tech Development Zone, Changsha City, Hunan Province, 410221 Patentee after: Changsha feichuang Biotechnology Co.,Ltd. Address before: 410004 No. 498 South Shaoshan Road, Hunan, Changsha Patentee before: CENTRAL SOUTH University OF FORESTRY AND TECHNOLOGY |