CN106483212A - The urine estrogen metabolism thing mark related to idiopathic male infertility and its detection method and application - Google Patents
The urine estrogen metabolism thing mark related to idiopathic male infertility and its detection method and application Download PDFInfo
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- CN106483212A CN106483212A CN201610844462.0A CN201610844462A CN106483212A CN 106483212 A CN106483212 A CN 106483212A CN 201610844462 A CN201610844462 A CN 201610844462A CN 106483212 A CN106483212 A CN 106483212A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
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Abstract
The invention belongs to analytical chemistry and clinical medicine domain, disclose the urine estrogen metabolism thing mark related to idiopathic male infertility and its detection method and application.The mark is 2 methoxyestradiol of urine estrogen metabolism thing and/or androstenedione, detected using UPLC Q exactive MS method, the mark can be used for the auxiliary diagnosis of idiopathic male infertility and monitoring, with higher sensitivity and specificity, with clinical generalization value.
Description
Invention field
The invention belongs to analytical chemistry and clinical medicine domain, are related to the urine estrogen related to idiopathic male infertility
Metabolite markers and its detection method based on UPLC-Q exactive MS and application.
Background technology
At present, the whole world there are about the couple at child-bearing age of 10-15% with growing barrier.China due to large population base, newly-married husband
In woman, infertile patient numbers exceed well over million, wherein male factor cause sterile be higher than 50%.Mesh in male sterility
Front still have about 40%～75% to can not find reason, referred to as idiopathic male infertility disease.Idiopathic male infertility causes extremely tight
The Disease Spectrum of weight.
It is true that there is great difficulty in idiopathic male infertility diagnosis.The diagnostic criteria of the WHO of male sterility is man and wife's wedding
After live together more than 1 year, do not take contraceptives, as bridegroom's or husband's side reason causes the infertile person in the wife's side.However, as practical reasons are a lot
Man and wife does not ensure that strict living together 1 year so that last male sterility judges to become extremely difficult；Live together up to 1 year
Observing time, the time for early treatment being carried out to male sterility and being intervened has been delayed significantly；In order to exclude wife's side reason, the wife's side is also
Need detailed inspection is carried out, bring heavy economy and medical burden.What existing male sterility coherence check was relied on is
Traditional Sperm recovery, it only focus on the conventional parameters such as sperm quantity, degrees of motion, semen volume, pH and liquefying time.Because
Affected by factors such as abstinence time, Sperm parameters conventional analysis result shows as larger fluctuation.Thus, clinical
Diagnosis generally requires, with reference to multiple semen routine analysis, to bring burden to doctor and patient.What is more important, traditional essence
Whole situations of seminal fluid can not be comprehensively reacted in liquid parameter testing.Therefore, male sterility often shows as Sperm parameters nothing
Substantially abnormal, also so that traditional seminal parameters inspection can not efficient diagnosis male sterility.Thus, clinically need badly for spy
The new diagnostic method of the property sent out male sterility.
Metabolism group is an emerging omics technology after genomics, protein science, the transcription group, and it is to pass through
High flux investigates a section of living things system Changeement living things system of metabolite after hereditary change and/or environment change
Learn.Metabolism group research is with " unbiased ", " overall situation ", " nothing hypothesis ", " being assumed by data-driven ", thus the knot of research
By often with higher reliability and novelty.Metabolism group with systematic overview, analysis and can explore cell, tissue and device
Small molecule biomarkers thing and its situation of change that official produces, are more visually known the material base of function phenotypic alternation, its
Object is related to the metabolism network of the organisms such as lipid metaboli, glycometabolism, amino acid metabolism, nucleic acid metabolism, coenzyme metabolism, is to compare gene
Group, protein science, the group of transcription group closer " phenotype ".Sample that metabolism group is analyzed due to which can for urine this
Noninvasive sample, can greatly reduce the wound of patient's sampling a bit.Metabolism group shows high in the diagnosis of complex disease
Application potential and value are high and stable have the characteristics that sensitivity.Metabolism group is by carrying out to the overall metabolic components of body
Analysis and research, therefrom find out specific biomarkers, can carry out timely, accurate, high sensitivity to clinical disease and height is special
The diagnosis of property.At present metabolism group be mainly used in terms of the diagnosis of disease coronary heart disease, liver diseases, diabetes, hypertension,
Fat and tumour.It should be noted that urine is the biological specimen being clinically readily available, with noninvasive, bulky advantage.
It is particularly suitable for diagnosis and the examination of disease.However, using metabonomic analysis urine metabolism small molecule in idiopathic male infertility
Diagnostic monitoring in application also paid close attention to accordingly.
Metabolism group detection platform common at present mainly includes nuclear magnetic resonance (NMR), gas-chromatography tandem mass spectrum (GC-
MS), Liquid Chromatography-Tandem Mass Spectrometry (LC-MS) and Capillary Electrophoresis tandem mass spectrum (CE-MS).Although nuclear magnetic resonance is that chemicals are fixed
Property goldstandard, but have the shortcomings that sensitivity is low, it is difficult to obtain more complete metabolism spectrum.It is high that tandem mass spectrum has sensitivity
With qualitative accurate advantage.But, gas-chromatography tandem mass spectrum is only limited to detect volatile material that detection range can not be covered
The requirement of metabolism spectrum.In order to make up the defect, gas-chromatography tandem mass spectrum often detects chemicals using the method for derivatization, this
So that pre-treating method becomes complicated, more errors are introduced.Meanwhile, gas-chromatography tandem mass spectrum is detected due to isolation technics
Limit, general detection time is longer, experimental period is extended, instrument occupancy is increased, limit which and examine in large sample crowd
Application in survey.Capillary Electrophoresis tandem mass spectrum, has an advantage to the separation detection of polar compound, but the asking of stability of instrument
Topic is relatively difficult to resolve certainly.And Liquid Chromatography-Tandem Mass Spectrometry has sample process simply, sensitivity is high, and the strong feature of Clinical practicability can
To solve the above problems well.UPLC-Q exactive MS is high resolution mass spectrum of new generation and the combining of ultra high efficiency liquid phase,
Have and compare the higher sensitivity of traditional LC-MS, specificity and stability.So urinated using UPLC-Q exactive MS
The metabonomic analysis of liquid metabolism small molecule, if in the stable special urine generation related to idiopathic male infertility morbidity, can be found
Thank to small molecule as biomarker, and research and develop the UPLC-Q exactive MS detection side of corresponding metabolism small molecule mark
Method, is not only in first place in the world in the field, can also create the economic benefit for attracting people's attention, to improving China's male genetic
The general level of the health also will be once strong promotion.
Content of the invention
It is an object of the invention to provide a kind of urine estrogen metabolism thing mark related to idiopathic male infertility.
Another object of the present invention is the detection method for providing above-mentioned urine estrogen metabolism thing mark.
A further object of the present invention is the kit for providing the above-mentioned urine estrogen metabolism thing mark of detection.
The purpose of the present invention is realized by following technical measures：
The urine estrogen metabolism thing mark related to idiopathic male infertility, the mark are urine estrogen metabolism
Thing methoxyestradiol and/or androstenedione.
Described urine estrogen metabolism thing mark is in idiopathic male infertility diagnosis or monitoring reagent box is prepared
Application.
A kind of diagnosis or the kit of monitoring idiopathic male infertility, the kit contain detection urine estrogen metabolism thing
Methoxyestradiol and/or the reagent of androstenedione.
Described kit, the kit contain using UPLC-Q exactive MS method detection urine estrogen metabolism
Thing methoxyestradiol and/or the reagent of androstenedione.
Described kit, the kit contain following reagent：
Methoxyestradiol standard items；
Androstenedione standard items；
Internal standard A：Creatinine, valine, nicotinic acid, thymidine, glutaric acid, the acid of L- phenylpropylamine, N- acetaminophen, horse
The Isotopic Internal Standard (deuterium mark, the aqueous solution) of one or more material of uric acid；
Internal standard B：The Isotopic Internal Standard (deuterium mark, methanol solution) of pentadecanoic acid；
Internal standard C：The Isotopic Internal Standard (deuterium mark, methanol solution) of lignoceric acid；
Further, the kit also contains：
Hypersil GOLD C18 chromatographic column；
Reagent A：100% methyl alcohol (protein precipitation use)；
Reagent B：The water (mobile phase use) of the formic acid containing 0.1%；
Reagent C：The acetonitrile (mobile phase use) of the formic acid containing 0.1%；
Reagent D：100% ultra-pure water (redissolves and uses).
A kind of method of the above-mentioned urine estrogen metabolism thing mark related to idiopathic male infertility of detection, which is special
Levy and be the method using UPLC-Q exactive MS method, detection urine estrogen metabolism thing methoxyestradiol and/or
The content of androstenedione.
In the method：
First, liquid-phase condition：
Liquid-phase chromatographic column is Hypersil GOLD C18 chromatographic column (100mm × 2.1mm, 1.9 μm of Thermo of particle diameter
Scientific, Germany), column temperature is 40 DEG C.
Mobile phase A is the water containing 0.1% formic acid, and Mobile phase B is the acetonitrile containing 0.1% formic acid, and flow velocity is 400 μ L/min；
Instrument gradient is：0-3min 1%B, 3-10min 1% arrive 99%B, 10-13min 99%B, 13-13.1min
99% arrives 1%B, 13.1-17min 1%B；(B refers to Mobile phase B, the amount of the amount of mobile phase A and corresponding Mobile phase B in each gradient
Totally 100%, similarly hereinafter)
Input mode：5 μ l of volume；
2nd, Mass Spectrometry Conditions
Using heating electron spray ionisation mode (HESI), positive ion mode spray voltage：3.5kV；Negative ion mode is sprayed
Voltage：2.5kV；Capillary temperature under both of which：250 DEG C, heter temperature：425 DEG C, sheath gas air-flow：50AU, aids in gas gas
Stream：13AU, blowback air air-flow：0AU；Lens voltage：60V.Using sweeping full pattern, sweep limits：70 arrive 1050m/z；Resolution ratio：
70000.
The present invention is described in detail as follows：
The present inventor gathers standard compliant urine specimen with S.O.P. (SOP), the complete crowd of systematic collection
Back ground Information and clinical data, and employ and be analyzed based on the metabolism group method of UPLC-Q exactive MS.
The experimental technique that specifically studies mainly includes following components：
First, research object is selected and packet foundation
First stage：Screening stage
Idiopathic male infertility 607 people and normal healthy controls 430 people that clarify a diagnosis is included at random, totally 1037 people.
A group：Healthy control group (430 people)：
1. the age is between 19 to 39 years old；
2. constitutional index is between 17 to 31；
3. healthy offspring is had after the male sex of fecundity health, and 5-8 month；
4. nothing whole body major disease.
B group：Idiopathic male infertility disease group (607 people)：
1. the age is mated with control group；
2. constitutional index is mated with control group
3. attempting pregnancy does not have success in 12 months, and spouse does not have the male sex of infertile disease；.
4. Wu the clear and definite male sterility cause of disease；
5. Smoking And Drinking history is mated with control group；
6. nationality is mated with control group；
7. nothing whole body major disease.
Second stage：Qualify Phase
15 people of idiopathic male infertility and 15 people of normal healthy controls for clarifying a diagnosis is included, totally 30 people.
A group：Healthy control group (15 people)：
1. the age is between 24 to 36 years old；
2. constitutional index is between 19 to 24；
3. healthy offspring is had after the male sex of fecundity health, and 5-8 month；
4. nothing whole body major disease.
B group：Idiopathic male infertility disease group (15 people)：
1. the age is mated with control group；
2. constitutional index is mated with control group；
3. attempting pregnancy does not have success in 12 months, and spouse does not have the male sex of infertile disease；
4. Wu the clear and definite male sterility cause of disease；
5. Smoking And Drinking history is mated with control group；
6. nationality is mated with control group；
7. nothing whole body major disease.
2nd, UPLC-Q exactive MS metabonomic analysis and idiopathic male infertility diagnosis are sieved with estrogen metabolism thing
Choosing and checking
1. Sample pretreatment
1. take 300 μ L urines, 10 μ L internal standards A are added, 10 μ L internal standards B are added, 10 μ L internal standards C are added, add 900 μ L of methyl alcohol
(reagent A), vortex 30s.
2. in centrifuge, 4 DEG C of 16000g are centrifuged 15min, supernatant is transferred to 1.5mL import EP pipe, and supernatant is existed
It is concentrated to dryness in centrifugal concentrating drying instrument under room temperature condition.
3. redissolved with 10 μ L ultra-pure water (reagent D), to be analyzed.
2. instrument detection
1. analytical instrument：UPLC Ultimate 3000system (Dionex) high performance liquid chromatograph；Q-Exactive
High-resolution mass spectrometer.
2. liquid-phase condition：
2.1. liquid-phase chromatographic column is Hypersil GOLD C18 chromatographic column (100mm × 2.1mm, 1.9 μm of particle diameter, Thermo
Scientific, Germany), column temperature is 40 DEG C.
2.2 mobile phases for adopting are the water (reagent B) of (A) containing 0.1% formic acid and the acetonitrile (reagent of (B) containing 0.1% formic acid
C), flow velocity is 400 μ L/min.
2.3 instrument gradients are：Instrument gradient is：0-3min 1%B, 3-10min 1% arrive 99%B, 10-13min 99%
B, 13-13.1min 99% arrive 1%B, 13.1-17min 1%B.
2.4 input mode：5 μ l of volume.
3. Mass Spectrometry Conditions
3.1 heating electron spray ionisation mode (HESI) are analyzed.
3.2 using heating electron spray ionisation mode (HESI), positive ion mode spray voltage：3.5kV；Negative ion mode sprays
Mist voltage：2.5kV；Capillary temperature under both of which：250 DEG C, heter temperature：425 DEG C, sheath gas air-flow：50AU, aids in gas
Air-flow：13AU, blowback air air-flow：0AU；Lens voltage：60V.Using sweeping full pattern, sweep limits：70 arrive 1050m/z；Differentiate
Rate：70000.
3. material is qualitative
Estrogen metabolism thing is qualitative to compare Chromatographic information (during reservation using with standard items methoxyestradiol and androstenedione
Between) and Information in Mass Spectra (accurate molecular weight), and the Chromatographic information of Isotopic Internal Standard standard items series in sample is compared in real time with school
Positive retention time.
4. data analysis：
Biomarker screening confirms key metabolites using Multivariate Logistic Regression.
5. the difference of estrogen metabolism thing and diagnostic significance in healthy control group, idiopathic male infertility group urine specimen.
The information of corrected age, constitutional index, smoking and history of drinking history, logistic regression find urine
The content increase of methoxyestradiol and androstenedione significantly improves the generation of idiopathic male infertility.Should using random crowd
With above-mentioned estrogen metabolism thing combined diagnosis idiopathic male infertility, sensitivity is 93.33%, and specificity is 93.33%, ROC
TG-AUC is 0.9867, with higher additive diagnostic value.
3rd, diagnostic reagent box preparation method
According to above-mentioned series of experiments result, the present inventor is also prepared for a kind of diagnosis or monitors idiopathic male infertility
Kit, the diagnostic kit is comprising stable existence and detectable methoxyestradiol and androstene in measure experimenter's urine
The internal standard standard items series of the standard items of diketone and assistant analysis.Diagnostic kit can also include a set of urine estrogen generation
Thank to thing to extract and used in chromatograph reagent and equipment.
Beneficial effects of the present invention：
The present inventor is by being compared in normal control and idiopathic male infertility urine using UPLC-Q exactive MS
Metabolism small molecule, it was found that in urine exist can be used to assess whether with idiopathic male infertility, with auxiliary diagnosis valency
The urine estrogen metabolism thing mark combination of value, and the UPLC-Q exactive of the estrogen metabolism thing marker detection
The application of MS, develop the idiopathic male infertility of clinical practice can be easy to diagnose, monitoring reagent box.
Mark of the present invention using urine metabolism small molecule as idiopathic male infertility evaluation is advantageous in that：
(1) urine metabolism small molecule is a kind of new biomarkers, and which is associated with disease outcome by force, not only stably, no
Wound, it is easy to detection, and quantitative accurate, will greatly improves the Sensitivity and Specificity of idiopathic male infertility diagnosis, such little point
Preventing and treating for idiopathic male infertility is started brand-new situation by the successful exploitation of sub- biomarker, is other diseases biological marker
The development of thing is offered reference.
(2) the urine metabolism small molecule mark that the present invention is provided can be used as the diagnosis marker of idiopathic male infertility,
Can in Check-up crowd clear and definite idiopathic male infertility disease condition, so as to for the further testing in depth testing of clinician provide according to
According to being quick and precisely to grasp the morbid state of patient and coincident with severity degree of condition, take the control prece of more personalized to carry in time
For supporting, delay and prevent progression of disease.
(3) present invention is verified using the urine specimen of idiopathic male infertility and the random crowd of normal healthy controls, it was demonstrated that
In urine, methoxyestradiol and Androstenedione levels are with higher sensitivity and special in diagnosis idiopathic male infertility
Degree, can use as mark.
(4) using tight, multistage checking and appraisement system, the initial stage screens multiple urine generations by preliminary experiment to the present invention
Thank to small molecule, application UPLC-Q exactive MS carries out independent crowd's checking, it is ensured that the urine metabolism biomarker and
The reliability of diagnostic method.
(5) UPLC-Q exactive MS technology sample process is simple, and Instrumental Analysis is accurate rapidly, with higher clinic
Diagnosis practical value.
Description of the drawings
Fig. 1 screening stage, the information of corrected age, constitutional index, smoking and history of drinking history, Multivariate Logistic Regression
Analysis finds that the content increase of urine methoxyestradiol and androstenedione significantly improves the generation of idiopathic male infertility.a
The single factor test Logistic regression result of Confounding Factor is not adjusted.bMany after adjustment age, constitutional index, smoking and history of drinking history
First Logistic regression result.
The horizontal fluctuation of Fig. 2 estrogen metabolism analyte detection (mean ± standard deviation).
Fig. 3 Qualify Phase, the Normal group made using urine methoxyestradiol content information and the idiopathic male sex
ROC curve between sterile group.
Fig. 4 Qualify Phase, the Normal group made using urine androstenedione content information and idiopathic male infertility
ROC curve between group.
Fig. 5 Qualify Phase, the Normal group made using urine methoxyestradiol and androstenedione content information and
ROC curve between idiopathic male infertility group.
Specific embodiment
The invention will be further elaborated by the following examples.
1 research object of embodiment is selected and packet foundation
This part research object examines idiopathic male infertility case and health from the head of affiliated hospital of Nanjing Medical University
Fertility control.Research contents and Informed Consent Form all obtain the approval of Ethics Committee of Nanjing Medical University, meet relevant laws and regulations
Requirement.Case and to impinge upon understand content after endorsed Informed Consent Form.All research objects have all carried out complete physique
Check, and complete a including personal basic data, habits and customs, occupation and environmental exposure, genetic risk factors, sexual function
Questionnaire with reproductive function, history of disease and physical exertion.First stage incorporates satisfactory 607 idiopathic male sex
Sterile case and 430 normal healthy controls；The satisfactory 15 idiopathic male infertility cases of second stage and 15 health right
According to the screening experiment object as idiopathic male infertility urine metabolism small molecule biomarker.Specific sample group standard
As follows：
First stage screening stage
Idiopathic male infertility 607 people and normal healthy controls 430 people that clarify a diagnosis is included at random, totally 1037 people.
A group：Healthy control group (430 people)：
1. the age is between 19 to 39 years old；
2. constitutional index is between 17 to 31；
3. healthy offspring is had after the male sex of fecundity health, and 5-8 month；
4. nothing whole body major disease.
B group：Idiopathic male infertility disease group (607 people)：
1. the age is mated with control group；
2. constitutional index is mated with control group
3. attempting pregnancy does not have success in 12 months, and spouse does not have the male sex of infertile disease；.
4. Wu the clear and definite male sterility cause of disease；
5. Smoking And Drinking history is mated with control group；
6. nationality is mated with control group；
7. nothing whole body major disease.
Second stage Qualify Phase
15 people of idiopathic male infertility and 15 people of normal healthy controls for clarifying a diagnosis is included, totally 30 people.
A group：Healthy control group (15 people)：
1. the age is between 24 to 36 years old；
2. constitutional index is between 19 to 24；
3. healthy offspring is had after the male sex of fecundity health, and 5-8 month；
4. nothing whole body major disease.
B group：Idiopathic male infertility disease group (15 people)：
1. the age is mated with control group；
2. constitutional index is mated with control group；
3. attempting pregnancy does not have success in 12 months, and spouse does not have the male sex of infertile disease；
4. Wu the clear and definite male sterility cause of disease；
5. Smoking And Drinking history is mated with control group；
6. nationality is mated with control group；
7. nothing whole body major disease.
Embodiment 2：UPLC-MS metabolism group idiopathic male infertility biomarker is screened
1. Sample pretreatment
1. take 300 μ L urines, 10 μ L internal standards A are added, 10 μ L internal standards B are added, 10 μ L internal standards C are added, add 900 μ L of methyl alcohol
(reagent A), vortex 30s.
2. in centrifuge, 4 DEG C of 16000g are centrifuged 15min, supernatant is transferred to 1.5mL import EP pipe, and supernatant is existed
It is concentrated to dryness in centrifugal concentrating drying instrument under room temperature condition.
3. redissolved with 10 μ L ultra-pure water (reagent D), to be analyzed.
2. instrument detection
1. analytical instrument：UPLC Ultimate 3000system (Dionex) high performance liquid chromatograph；Q-Exactive
High-resolution mass spectrometer.
2. liquid-phase condition：
2.1. liquid-phase chromatographic column is Hypersil GOLD C18 chromatographic column (100mm × 2.1mm, 1.9 μm of particle diameter, Thermo
Scientific, Germany), column temperature is 40 DEG C.
2.2 mobile phases for adopting are the water (reagent B) of (A) containing 0.1% formic acid and the acetonitrile (reagent of (B) containing 0.1% formic acid
C), flow velocity is 400 μ L/min.
2.3 instrument gradients are：Instrument gradient is：0-3min 1%B, 3-10min 1% arrive 99%B, 10-13min 99%
B, 13-13.1min 99% arrive 1%B, 13.1-17min 1%B.
2.4 input mode：5 μ l of volume.
3. Mass Spectrometry Conditions
3.1 heating electron spray ionisation mode (HESI) are analyzed.
3.2 using heating electron spray ionisation mode (HESI), positive ion mode spray voltage：3.5kV；Negative ion mode sprays
Mist voltage：2.5kV；Capillary temperature under both of which：250 DEG C, heter temperature：425 DEG C, sheath gas air-flow：50AU, aids in gas
Air-flow：13AU, blowback air air-flow：0AU；Lens voltage：60V.Using sweeping full pattern, sweep limits：70 arrive 1050m/z；Differentiate
Rate：70000.
3. material is qualitative
Estrogen metabolism thing is qualitative to compare Chromatographic information (during reservation using with standard items methoxyestradiol and androstenedione
Between) and Information in Mass Spectra (accurate molecular weight), and the Chromatographic information of Isotopic Internal Standard standard items series in sample is compared in real time with school
Positive retention time.
4. data analysis：
Biomarker screening confirms key metabolites using Multivariate Logistic Regression.
5. the difference of estrogen metabolism thing and diagnostic significance in healthy control group, idiopathic male infertility group urine specimen.
The information of corrected age, constitutional index, smoking and history of drinking history, logistic regression find urine
The content increase of methoxyestradiol and androstenedione significantly improves the generation (Fig. 1) of idiopathic male infertility.
The stability analysis of 3 urine estrogen metabolism thing of embodiment
The stability of urine methoxyestradiol and Androstenedione levels is evaluated using the method for embodiment 2 (
It it is 2 weeks every the time).As a result show, in urine, methoxyestradiol and androstenedione determine horizontal stable (Fig. 2), possess conduct
The characteristic of diagnosis/monitoring mark.
4 estrogen metabolism thing of embodiment combines the diagnosis to idiopathic male infertility
According to above-mentioned UPLC-Q exactive MS metabolism group method, the present inventor by 15 case of random crowd and
The urine sample detection methoxyestradiol of 15 controls and androstenedione, draw ROC curve with this and assess the sensitivity of diagnosis
And specificity, and then assess this evaluation capacity of 2 estrogen metabolism thing levels to idiopathic male infertility in detection urine.
The sensitivity of methoxyestradiol is 86.67%, and specificity is 80.00%, and under ROC curve, area is 0.9022
(Fig. 3)；
Androstenedione sensitivity is 86.67%, and specificity is 86.67%, and under ROC curve, area is 0.93333 (Fig. 4)；
The sensitivity of combination methoxyestradiol and androstenedione is 93.33%, and specificity is 93.33%, under ROC curve
Area is 0.9867 (Fig. 5).
So combination methoxyestradiol and androstenedione have the ability for preferably diagnosing idiopathic male infertility.
Embodiment 5 is used for the making of idiopathic male infertility urine estrogen metabolism analyte detection and diagnostic kit
Determine in normal control and idiopathic male infertility urine have by the method for UPLC-Q exactive MS first
There is more abundant metabolism small molecule.Then, sieved by the metabonomic technology based on UPLC-Q exactive MS wherein
The choosing estrogen metabolism thing related to idiopathic male infertility, used as the diagnosis index for being whether idiopathic male infertility.Finally
The quantity of the corresponding urine estrogen metabolism thing for filtering out is controlled at 2, this is the optimum that makes on the basis of preliminary experiment
That changed simplifies.Using this 2 estrogen metabolism things, preferable sensitivity and specificity can be both ensured, and can be cost-effective, subtract
The burden of light patient, moreover it is possible to reduce detection time, have the advantages that quick, accurate, economical, is easy to clinical expansion to use, certainly
Using wherein 1 mark can also, more preferable using 2 mark effects.This kit includes a collection of urine estrogen metabolism
The qualitative and quantitative mark for adopting methoxyestradiol and androstenedione of analyte detection reagent and consumptive material, wherein estrogen metabolism thing
Quasi- product, assistant analysis adopt internal standard A：Creatinine, valine, nicotinic acid, thymidine, glutaric acid, the acid of L- phenylpropylamine, N- acetyl pair
Amino phenols, the deuterium mark Isotopic Internal Standard of eight kinds of materials of hippuric acid.Internal standard B：The deuterium mark Isotopic Internal Standard of pentadecanoic acid.Internal standard C：Two
The deuterium mark Isotopic Internal Standard of tetradecanoic acid.Other also have the supporting reverse chromatograms post (Hypersil for UPLC chromatographic isolation
GOLD C18 chromatographic column, 100mm × 2.1mm, 1.9 μm of particle diameter), (water of the formic acid containing 0.1% and contain for the reagent of mobile phase
The acetonitrile of 0.1% formic acid), for extracting the reagent (100% ultra-pure water) of estrogen metabolism thing.The value of this kit is
Only need to 300 μ l urines, you can the content of detection urine estrogen metabolism thing mark, then the idiopathic male sex is diagnosed by content
Sterile, and be easy to carry out dynamic monitoring and observation and treatment effect.
Concrete kit forms are as follows：
Methoxyestradiol standard items
Androstenedione standard items
Internal standard A：Creatinine, valine, nicotinic acid, thymidine, glutaric acid, the acid of L- phenylpropylamine, N- acetaminophen, horse
The deuterium mark Isotopic Internal Standard of eight kinds of materials of uric acid
Internal standard B：The deuterium mark Isotopic Internal Standard of pentadecanoic acid
Internal standard C：The deuterium mark Isotopic Internal Standard of lignoceric acid
Further, can also contain：
Chromatographic column (Thermo 100mm × 2.1mm, 1.9 μm of particle diameter, Hypersil GOLD C18 chromatographic column)
Reagent A (containing 100% methyl alcohol)
Reagent B (water of the formic acid containing 0.1%)
Reagent C (acetonitrile of the formic acid containing 0.1%)
Reagent D (100% ultra-pure water).
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Claims (8)
1. the urine estrogen metabolism thing mark related to idiopathic male infertility, it is characterised in that the mark is that urine is female
Hormone metabolism thing methoxyestradiol and/or androstenedione.
2. the urine estrogen metabolism thing mark described in claim 1 is preparing idiopathic male infertility diagnosis or monitoring reagent
Application in box.
3. a kind of diagnosis or monitoring idiopathic male infertility kit, it is characterised in that the kit contain detection urine female swash
Plain metabolin methoxyestradiol and/or the reagent of androstenedione.
4. kit according to claim 3, it is characterised in that the kit contains using UPLC-Q exactive MS
Method detection urine estrogen metabolism thing methoxyestradiol and/or the reagent of androstenedione.
5. kit according to claim 4, it is characterised in that the kit contains following reagent：
Methoxyestradiol standard items；
Androstenedione standard items；
Internal standard A：Creatinine, valine, nicotinic acid, thymidine, glutaric acid, the acid of L- phenylpropylamine, N- acetaminophen, hippuric acid
The Isotopic Internal Standard of one or more material；
Internal standard B：The Isotopic Internal Standard of pentadecanoic acid；
Internal standard C：The Isotopic Internal Standard of lignoceric acid；
6. kit according to claim 5, it is characterised in that the kit also contains：
Hypersil GOLD C18 chromatographic column；
Reagent A：100% methyl alcohol；
Reagent B：The water of the formic acid containing 0.1%；
Reagent C：The acetonitrile of the formic acid containing 0.1%；
Reagent D：100% ultra-pure water.
7. a kind of detection related to idiopathic male infertility as claimed in claim 1 urine estrogen metabolism thing mark
Method, it is characterised in that the method adopts UPLC-Q exactive MS method, detection urine estrogen metabolism thing 2- methoxy is female
Glycol and/or the content of androstenedione.
8. method according to claim 7, it is characterised in that in the method：
First, liquid-phase condition：
Liquid-phase chromatographic column is Hypersil GOLD C18 chromatographic column, and column temperature is 40 DEG C.
Mobile phase A is the water containing 0.1% formic acid, and Mobile phase B is the acetonitrile containing 0.1% formic acid, and flow velocity is 400 μ L/min；
Instrument gradient is：0-3min 1%B, 3-10min 1% arrive 99%B, 10-13min 99%B, 13-13.1min 99%
To 1%B, 13.1-17min 1%B；
Input mode：5 μ l of volume；
2nd, Mass Spectrometry Conditions
Using heating electron spray ionisation mode (HESI), positive ion mode spray voltage：3.5kV；Negative ion mode spray voltage：
2.5kV；Capillary temperature under both of which：250 DEG C, heter temperature：425 DEG C, sheath gas air-flow：50AU, aids in gas air-flow：
13AU, blowback air air-flow：0AU；Lens voltage：60V.Using sweeping full pattern, sweep limits：70 arrive 1050m/z；Resolution ratio：
70000.
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