CN106290653B - With the relevant urine fatty acid metabolism object marker of idiopathic male infertility and its detection method and application - Google Patents
With the relevant urine fatty acid metabolism object marker of idiopathic male infertility and its detection method and application Download PDFInfo
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Abstract
The invention belongs to analytical chemistry and clinical medicine domain, disclose and the relevant urine fatty acid metabolism object marker of idiopathic male infertility and its detection method and application.The marker is that the marker is urine fatty acid metabolism object tetracosandioic acid and/or hexadecandioic acid (hexadecane diacid), it is detected using UPLC Q exactive MS methods, the marker can be used for auxiliary diagnosis and the monitoring of idiopathic male infertility, have higher sensitivity and specificity, have clinical generalization value.
Description
Invention field
The invention belongs to analytical chemistry and clinical medicine domain, it is related to and the relevant urine aliphatic acid of idiopathic male infertility
Metabolite markers and its detection method based on UPLC-Q exactive MS and application.
Background technology
The whole world there are about 15% couple at child-bearing age by infertility perplexed.China is due to large population base, in newly-married couple
For infertile patient numbers far more than million, the infertility caused by male factor is higher than 50%.Still have at present in male sterility
About 40%~75% can not find reason, referred to as idiopathic male infertility disease.Idiopathic male infertility causes extremely serious disease
Disease burden.
However, idiopathic male infertility diagnosis is extremely difficult.The standard of WHO male sterilities be man and wife live together after marriage 1 year with
On, contraceptives are not taken, and the infertile person in the wife's side is caused due to the bridegroom's or husband's side.However, the diagnostic criteria lived together 1 year, delays significantly
The time of early treatment and intervention is carried out to male sterility；Since many men and wives of practical reasons do not ensure that stringent live together
1 year so that last male sterility judgement becomes extremely difficult；And in order to exclude wife's side reason, the wife's side also needs to carry out detailed
Thin inspection brings heavy economy and medical burden.Traditional seminal parameters inspection only focus on sperm quantity, degrees of motion,
The conventional parameters such as semen volume, pH and liquefying time cannot comprehensively react whole situations of sperm.Therefore, male sterility is frequent
Sperm parameters Non Apparent Abnormality is shown as, but also traditional seminal parameters inspection can not efficient diagnosis male sterility.
In addition to this because being influenced by factors such as abstinence times, Sperm parameters conventional analysis result is also embodied by larger wave
Dynamic property.Thus, clinical diagnosis generally requires to refer to multiple semen routine analysis, and burden is brought to doctor and patient.Thus, face
There is an urgent need for the new diagnostic methods for idiopathic male infertility on bed.
Metabolism group (Metabolomics/Metabonomics) is grow up phase late 1990s one new
Set up schools section, it be by investigate biosystem hereditary change it is stimulated or disturbance after, the variation of metabolite or its with
The variation of time, to study a science of biosystem.So-called metabolism group (Metabolome) is the downstream product of genome
It is also final product, is the small molecule that some participate in organism metabolism, maintain organism normal function and growth and development
The set of object is closed, mainly relative molecular mass is less than 1000 endogenous small molecule, these endogenous metabolism small molecules are related to
Glycometabolism, energetic supersession, lipid metaboli, amino acid metabolism, nucleic acid metabolism, coenzyme metabolism etc..
Organism under normal condition is a complete system, and the metabolin in biological fluid, cell and tissue is in
One stable equilibrium state.Pathological change will have occurred due to heredity or the day after tomorrow in body, this balance is just broken, generation
It thanks product and metabolic process and also produces corresponding variation.Small molecule metabolism is understood in lysis by metabonomic analysis
Variation, people can be helped to find related biomarker (biomarker), can be with the diagnosis of aided disease, can also
It helps the metabolic pathway that people are related to by small-molecule substance itself to understand the pathogenesis of disease and provides spy for medicament research and development
Anisotropic target.In recent years, metabolism group achieves in the research of mankind's various diseases in the early diagnosis of disease many
The achievement in research being of great significance, such as angiocardiopathy, diabetes and cancer, correlative theses are published in academic journal
《Nature》、《Nature medicine》、《Journal of hepatology》With《Cancer research》On, show
Metabolism small molecule huge potentiality and value in diagnosing human disease.It is worth noting that, urine is clinically easy obtains
The biological sample obtained, has the advantages that noninvasive, bulky.It is particularly suitable for the diagnosis and screening of disease.However, using metabolism group
Application of the urine metabolism small molecule in the diagnostic monitoring of idiopathic male infertility is analyzed not paid close attention to accordingly also.
The common technology of metabolism group research at present includes liquid chromatograph mass spectrography (LC-MS), chromatography of gases-mass spectrum connection
With (GC-MS) and nuclear magnetic resonance technique (NMR).Nuclear magnetic resonance technique feature is to component to be measured without destruction, Sample pretreatment letter
It is single, but sensitivity is relatively low；Gas chromatography-mass spectrography has preferable sensitivity and reproducibility, but generally to use derivatization
Method carries out pre-treatment to sample so that experimental procedure becomes complicated.And LC-MS has sample process simple, high sensitivity is faced
The highly practical feature of bed.UPLC-Q exactive MS are the combinations of high resolution mass spectrum of new generation and ultra high efficiency liquid phase, are had
Compared to the stronger sensitivity of traditional LC-MS, specificity and stability.So carrying out urine using UPLC-Q exactive MS
Metabonomic analysis, if can find, stable being used as with the relevant special urine metabolism small molecule of idiopathic male infertility morbidity is given birth to
Object marker, and the UPLC-Q exactive MS detection methods of corresponding metabolism small molecule mark are researched and developed, not only in the field
In first place in the world, the economic benefit to attract people's attention can be also created, will be to improving China's male reproductive health level also
Primary strong promotion.
Invention content
The object of the present invention is to provide with the relevant urine fatty acid metabolism object marker of idiopathic male infertility.
Another object of the present invention is to provide the detection method of above-mentioned urine fatty acid metabolism object marker.
A further object of the present invention is to provide the kit for detecting above-mentioned urine fatty acid metabolism object marker.
The purpose of the present invention is what is realized by following technical measures：
With the relevant urine fatty acid metabolism object marker of idiopathic male infertility, which is urine fatty acid metabolism
Object tetracosandioic acid and/or hexadecandioic acid (hexadecane diacid).
The urine fatty acid metabolism object marker is in preparing idiopathic male infertility diagnosis or monitoring reagent box
Using.
A kind of kit diagnosed or monitor idiopathic male infertility, the kit contain detection urine fatty acid metabolism object
The reagent of tetracosandioic acid and/or hexadecandioic acid (hexadecane diacid).
The kit, which contains detects urine fatty acid metabolism using UPLC-Q exactive MS methods
The reagent of object tetracosandioic acid and/or hexadecandioic acid (hexadecane diacid).
The kit, the kit contain following reagent：
Tetracosandioic acid standard items
Hexadecandioic acid (hexadecane diacid) standard items
Internal standard A：Creatinine, valine, niacin, thymidine, glutaric acid, L- phenylpropylamines acid, N- acetaminophens, horse
The Isotopic Internal Standard (deuterium mark, aqueous solution) of one or more substances in uric acid；
Internal standard B：The Isotopic Internal Standard (deuterium mark, methanol solution) of pentadecanoic acid；
Internal standard C：The Isotopic Internal Standard (deuterium mark, methanol solution) of lignoceric acid.
Further, which also contains：
Hypersil GOLD C18 chromatographic columns；
Reagent A：Containing 100% methanol (protein precipitation use)；
Reagent B：Water (mobile phase use) containing 0.1% formic acid；
Reagent C：Acetonitrile (mobile phase use) containing 0.1% formic acid；
Reagent D：100% ultra-pure water (is redissolved and is used).
A method of detection is above-mentioned with the relevant urine fatty acid metabolism object marker of idiopathic male infertility, the party
Method uses UPLC-Q exactive MS methods, detects urine fatty acid metabolism object tetracosandioic acid and/or hexadecandioic acid (hexadecane diacid)
Content.
In this method：
One, liquid-phase condition：
Liquid-phase chromatographic column is Hypersil GOLD C18 chromatographic columns, and column temperature is 40 DEG C；
Mobile phase A is the water containing 0.1% formic acid, and Mobile phase B is the acetonitrile containing 0.1% formic acid, and flow velocity is 400 μ L/min；
Instrument gradient is：0-3min 1%B, 3-10min 1% arrives 99%B, 10-13min 99%B, 13-13.1min
99% arrives 1%B, 13.1-17min 1%B；(B refers to Mobile phase B, the amount of the amount and corresponding Mobile phase B of mobile phase A in each gradient
Totally 100%, similarly hereinafter)
Input mode：5 μ l of volume；
Two, Mass Spectrometry Conditions
It is analyzed using heating electron spray ionisation mode (HESI), positive ion mode spray voltage：3.5kV；Anion
Pattern spray voltage：2.5kV；Capillary temperature under both of which：250 DEG C, heter temperature：425 DEG C, sheath gas air-flow：50AU,
Assist gas air-flow：13AU, blowback air air-flow：0AU；Lens voltage：60V；Using sweeping pattern, scanning range entirely：70 arrive 1050m/
z；Resolution ratio：70000.
The present invention is described in detail as follows：
The present inventor acquires standard compliant urine specimen with S.O.P. (SOP), and system collects complete crowd
Basic information and clinical data, and use the metabolism group method based on UPLC-Q exactive MS and analyzed.
The experimental method specifically studied includes mainly following components：
One, research object selection and grouping foundation
First stage screening stage
It is included in 430 people of 607 people of idiopathic male infertility and normal healthy controls to clarify a diagnosis at random, totally 1037 people.
A groups：Healthy control group (430 people)：
1. the age is between 19 to 39 years old；
2. constitutional index is between 17 to 31；
3. the male of fecundity health, and have healthy offspring after 5-8 months；
4. without whole body major disease.
B groups：Idiopathic male infertility disease group (607 people)：
1. the age matches with control group；
2. constitutional index is matched with control group
3. pregnancy is attempted not succeed within 12 months, and spouse does not have the male of infertile disease；.
4. without the clear male sterility cause of disease；
5. Smoking And Drinking history is matched with control group；
6. nationality matches with control group；
7. without whole body major disease.
Second stage Qualify Phase
It is included in 15 people of 15 people of idiopathic male infertility and normal healthy controls to clarify a diagnosis, totally 30 people.
A groups：Healthy control group (15 people)：
1. the age is between 24 to 36 years old；
2. constitutional index is between 19 to 24；
3. the male of fecundity health, and have healthy offspring after 5-8 months；
4. without whole body major disease.
B groups：Idiopathic male infertility disease group (15 people)：
1. the age matches with control group；
2. constitutional index is matched with control group；
3. pregnancy is attempted not succeed within 12 months, and spouse does not have the male of infertile disease；
4. without the clear male sterility cause of disease；
5. Smoking And Drinking history is matched with control group；
6. nationality matches with control group；
7. without whole body major disease.
Two, UPLC-Q exactive MS metabonomic analysis and idiopathic male infertility diagnosis are sieved with fatty acid metabolism object
Choosing and verification
1. Sample pretreatment
1. taking 300 μ L urines, 10 μ L internal standard A being added, 10 μ L internal standard B are added, 10 μ L internal standard C are added, 900 μ L of methanol are added
(reagent A), vortex 30s.
2. 4 DEG C of 16000g centrifuge 15min in centrifuge, supernatant is transferred to 1.5mL imports EP pipes, and supernatant is existed
It is concentrated to dryness in centrifugal concentrating drying instrument under room temperature.
3. it is redissolved with 10 μ L ultra-pure waters (reagent D), it is to be analyzed.
2. instrument detects
1. analytical instrument：UPLC Ultimate 3000system (Dionex) high performance liquid chromatograph；Q-Exactive
High-resolution mass spectrometer.
2. liquid-phase condition：
2.1. liquid-phase chromatographic column is Hypersil GOLD C18 chromatographic columns (100mm × 2.1mm, 1.9 μm of grain size, Thermo
Scientific, Germany), column temperature is 40 DEG C.
2.2 mobile phases used contain the acetonitrile (reagent of 0.1% formic acid for the water (reagent B) of (A) containing 0.1% formic acid and (B)
C), flow velocity is 400 μ L/min.
2.3 instrument gradients are：Instrument gradient is：0-3min 1%B, 3-10min 1% arrives 99%B, 10-13min 99%
B, 13-13.1min 99% arrives 1%B, 13.1-17min 1%B.
2.4 input mode：5 μ l of volume.
3. Mass Spectrometry Conditions
3.1 heating electron spray ionisation modes (HESI) are analyzed.
3.2 using heating electron spray ionisation mode (HESI), positive ion mode spray voltage：3.5kV；Negative ion mode sprays
Mist voltage：2.5kV；Capillary temperature under both of which：250 DEG C, heter temperature：425 DEG C, sheath gas air-flow：50AU assists gas
Air-flow：13AU, blowback air air-flow：0AU；Lens voltage：60V.Using sweeping pattern, scanning range entirely：70 arrive 1050m/z；It differentiates
Rate：70000.
3. substance is qualitative
Fatty acid metabolism object is qualitative to compare Chromatographic information (when reservation using with standard items tetracosandioic acid and hexadecandioic acid (hexadecane diacid)
Between) and Information in Mass Spectra (accurate molecular weight), and the Chromatographic information of Isotopic Internal Standard standard items series in sample is compared in real time with school
Positive retention time.
4. data analysis：
Biomarker screening confirms key metabolites using Multivariate Logistic Regression.
5. the difference and diagnostic significance of fatty acid metabolism object in healthy control group, idiopathic male infertility group urine specimen.
The corrected age, constitutional index, smoking and history of drinking history information, logistic regression find urine
The content increase of tetracosandioic acid and hexadecandioic acid (hexadecane diacid) significantly improves the generation of idiopathic male infertility.It is answered using random crowd
With above-mentioned fatty acid metabolism object combined diagnosis idiopathic male infertility, sensitivity 93.33%, specificity 93.33%, ROC
Area under the curve is 0.9822, has higher additive diagnostic value.
Three, diagnostic reagent box preparation method
According to above-mentioned a series of experiments as a result, the present inventor be also prepared for it is a kind of can be used for idiopathic male infertility dynamic supervise
The diagnostic kit of survey, the diagnostic kit include to measure to be stabilized and detectable tetracosandioic acid in subject's urine
With the standard items of hexadecandioic acid (hexadecane diacid) and the internal standard standard items series of assistant analysis.Diagnostic kit can also include a set of urine
The extraction of fatty acid metabolism object and used in chromatograph reagent and equipment.
Beneficial effects of the present invention：
The present inventor compares by using UPLC-Q exactive MS in normal control and idiopathic male infertility urine
Metabolism small molecule, it was found that in urine exist can be used for assessing whether suffer from idiopathic male infertility, have auxiliary diagnosis valence
The urine fatty acid metabolism object marker of value combines and the UPLC-Q exactive of the fatty acid metabolism object marker detection
The idiopathic male infertility diagnosis that can be convenient for clinical application, monitoring reagent box are developed in the application of MS.
The present invention is advantageous in that using the marker that urine metabolism small molecule is evaluated as idiopathic male infertility：
(1) urine metabolism small molecule is a kind of new biomarkers, is associated with disease outcome by force, is not only stablized, nothing
It creates, be easy to detect, and is quantitative accurate, will greatly improve the sensibility and specificity of idiopathic male infertility diagnosis, such small point
The successful exploitation of sub- biomarker will start completely new situation for the prevention of idiopathic male infertility, be other diseases biological marker
The development of object is offered reference.
(2) urine metabolism small molecule marker provided by the invention can be used as the diagnosis marker of idiopathic male infertility,
Can in Check-up crowd clear idiopathic male infertility disease condition, to for the further testing in depth testing of clinician provide according to
According to quick and precisely to grasp the morbid state of patient and coincident with severity degree of condition, taking the control prece of more personalized to carry in time
For supporting, delay and prevent progression of disease.
(3) present invention is verified using the urine specimen of idiopathic male infertility and the random crowd of normal healthy controls, it was demonstrated that
Tetracosandioic acid and hexadecandioic acid (hexadecane diacid) level have higher sensitivity and special in diagnose idiopathic male infertility in urine
Degree can be used as marker use.
(4) present invention uses tight, multistage verification and appraisement system, initial stage to screen a variety of urine generations by preliminary experiment
Thank to small molecule, independent crowd's verification carried out using UPLC-Q exactive MS, ensure that the urine metabolism biomarker and
The reliability of diagnostic method.
(5) UPLC-Q exactive MS technology sample process is simple, and Instrumental Analysis is accurate rapidly, has higher clinic
Diagnose practical value.
Description of the drawings
Fig. 1 screening stages, the corrected age, constitutional index, smoking and history of drinking history information, Multivariate Logistic Regression
Analysis finds that the content increase of urine tetracosandioic acid and hexadecandioic acid (hexadecane diacid) significantly improves the generation of idiopathic male infertility.a
The single factor test Logistic regression results of Confounding Factor are not adjusted.bIt adjusts more after age, constitutional index, smoking and history of drinking history
First Logistic regression results.
The horizontal fluctuation of Fig. 2 fatty acid metabolism analyte detections (mean ± standard deviation).
Fig. 3 Qualify Phases, the Normal group made of urine tetracosandioic acid content information and idiopathic male are not
Educate the ROC curve between group.
Fig. 4 Qualify Phases, the Normal group made of urine hexadecandioic acid (hexadecane diacid) content information and idiopathic male are not
Educate the ROC curve between group.
Fig. 5 Qualify Phases, the Normal group made of urine tetracosandioic acid and hexadecandioic acid (hexadecane diacid) content information and
ROC curve between idiopathic male infertility group.
Specific implementation mode
The invention will be further elaborated by the following examples.
1 research object of embodiment selects and grouping foundation
Head of this part research object from affiliated hospital of Nanjing Medical University examines idiopathic male infertility case and health
Fertility control.Research contents and informed consent form obtain the approval of Ethics Committee of Nanjing Medical University, meet relevant laws and regulations
Requirement.Case and to impinge upon understand content after endorsed informed consent form.All research objects have carried out complete physique
It checks, and it includes personal basic data, living habit, occupation and environmental exposure, genetic risk factors, sexual function to complete a
With reproductive function, history of disease and the questionnaire of physical exertion.First stage incorporates satisfactory 607 idiopathic males
Sterile case and 430 normal healthy controls；The satisfactory 15 idiopathic male infertility cases of second stage and 15 health are right
According to the screening experiment object as idiopathic male infertility urine metabolism small molecule biomarker.Specific sample group standard
It is as follows：
First stage screening stage
It is included in 430 people of 607 people of idiopathic male infertility and normal healthy controls to clarify a diagnosis at random, totally 1037 people.
A groups：Healthy control group (430 people)：
1. the age is between 19 to 39 years old；
2. constitutional index is between 17 to 31；
3. the male of fecundity health, and have healthy offspring after 5-8 months；
4. without whole body major disease.
B groups：Idiopathic male infertility disease group (607 people)：
1. the age matches with control group；
2. constitutional index is matched with control group
3. pregnancy is attempted not succeed within 12 months, and spouse does not have the male of infertile disease；.
4. without the clear male sterility cause of disease；
5. Smoking And Drinking history is matched with control group；
6. nationality matches with control group；
7. without whole body major disease.
Second stage Qualify Phase
It is included in 15 people of 15 people of idiopathic male infertility and normal healthy controls to clarify a diagnosis, totally 30 people.
A groups：Healthy control group (15 people)：
1. the age is between 24 to 36 years old；
2. constitutional index is between 19 to 24；
3. the male of fecundity health, and have healthy offspring after 5-8 months；
4. without whole body major disease.
B groups：Idiopathic male infertility disease group (15 people)：
1. the age matches with control group；
2. constitutional index is matched with control group；
3. pregnancy is attempted not succeed within 12 months, and spouse does not have the male of infertile disease；
4. without the clear male sterility cause of disease；
5. Smoking And Drinking history is matched with control group；
6. nationality matches with control group；
7. without whole body major disease.
Embodiment 2UPLC-MS metabolism group idiopathic male infertility biomarkers screen
1. Sample pretreatment
1. taking 300 μ L urines, 10 μ L internal standard A being added, 10 μ L internal standard B are added, 10 μ L internal standard C are added, 900 μ L of methanol are added
(reagent A), vortex 30s.
2. 4 DEG C of 16000g centrifuge 15min in centrifuge, supernatant is transferred to 1.5mL imports EP pipes, and supernatant is existed
It is concentrated to dryness in centrifugal concentrating drying instrument under room temperature.
3. it is redissolved with 10 μ L ultra-pure waters (reagent D), it is to be analyzed.
2. instrument detects
1. analytical instrument：UPLC Ultimate 3000system (Dionex) high performance liquid chromatograph；Q-Exactive
High-resolution mass spectrometer.
2. liquid-phase condition：
2.1. liquid-phase chromatographic column is Hypersil GOLD C18 chromatographic columns (100mm × 2.1mm, 1.9 μm of grain size, Thermo
Scientific, Germany), column temperature is 40 DEG C.
2.2 mobile phases used contain the acetonitrile (reagent of 0.1% formic acid for the water (reagent B) of (A) containing 0.1% formic acid and (B)
C), flow velocity is 400 μ L/min.
2.3 instrument gradients are：Instrument gradient is：0-3min 1%B, 3-10min 1% arrives 99%B, 10-13min 99%
B, 13-13.1min 99% arrives 1%B, 13.1-17min 1%B.
2.4 input mode：5 μ l of volume.
3. Mass Spectrometry Conditions
3.1 heating electron spray ionisation modes (HESI) are analyzed.
3.2 using heating electron spray ionisation mode (HESI), positive ion mode spray voltage：3.5kV；Negative ion mode sprays
Mist voltage：2.5kV；Capillary temperature under both of which：250 DEG C, heter temperature：425 DEG C, sheath gas air-flow：50AU assists gas
Air-flow：13AU, blowback air air-flow：0AU；Lens voltage：60V.Using sweeping pattern, scanning range entirely：70 arrive 1050m/z；It differentiates
Rate：70000.
3. substance is qualitative
Fatty acid metabolism object is qualitative to compare Chromatographic information (when reservation using with standard items tetracosandioic acid and hexadecandioic acid (hexadecane diacid)
Between) and Information in Mass Spectra (accurate molecular weight), and the Chromatographic information of Isotopic Internal Standard standard items series in sample is compared in real time with school
Positive retention time.
4. data analysis：
Biomarker screening confirms key metabolites using Multivariate Logistic Regression.
5. the difference and diagnostic significance of fatty acid metabolism object in healthy control group, idiopathic male infertility group urine specimen.
The corrected age, constitutional index, smoking and history of drinking history information, logistic regression find urine
The content increase of tetracosandioic acid and hexadecandioic acid (hexadecane diacid) significantly improves the generation (Fig. 1) of idiopathic male infertility.
The stability analysis of 3 urine fatty acid metabolism object of embodiment
The stability of urine tetracosandioic acid and hexadecandioic acid (hexadecane diacid) level is evaluated using the method for embodiment 2 (
It it is 2 weeks every the time).The results show that tetracosandioic acid and hexadecandioic acid (hexadecane diacid) measure horizontal stable (Fig. 2) in urine, has conduct
The characteristic of diagnosis/monitoring marker.
4 fatty acid metabolism object of embodiment combines the diagnosis to idiopathic male infertility
According to above-mentioned UPLC-Q exactive MS metabolism group methods, the present inventor by 15 case of random crowd and
The urine sample detection tetracosandioic acid and hexadecandioic acid (hexadecane diacid) of 15 controls, with the sensitivity that this draws ROC curve and assesses diagnosis
And specificity, and then evaluation capacity of this 2 fatty acid metabolism object levels to idiopathic male infertility in assessment detection urine.
The sensitivity of tetracosandioic acid is 80.00%, specificity 80.00%, and area is 0.8489 (figure under ROC curve
3)；
Hexadecandioic acid (hexadecane diacid) sensitivity is 80.00%, specificity 80.00%, and area is 0.9289 (Fig. 4) under ROC curve；
The sensitivity for combining tetracosandioic acid and hexadecandioic acid (hexadecane diacid) is 93.33%, specificity 93.33%, under ROC curve
Area is 0.9822 (Fig. 5).
So combining tetracosandioic acid and hexadecandioic acid (hexadecane diacid) has the ability for preferably diagnosing idiopathic male infertility.
Embodiment 5 is used for the making of idiopathic male infertility urine fatty acid metabolism analyte detection and diagnostic kit
Determine in normal control and idiopathic male infertility urine have by the method for UPLC-Q exactive MS first
There is the metabolism small molecule compared with high abundance.Then, it is sieved wherein by the metabonomic technology based on UPLC-Q exactive MS
Choosing with the relevant fatty acid metabolism object of idiopathic male infertility, as whether be idiopathic male infertility diagnosis index.Finally
The quantity of the correspondence urine fatty acid metabolism object filtered out is controlled at 2, this be made on the basis of preliminary experiment it is optimal
That changes simplifies.Using this 2 fatty acid metabolism objects, it can not only ensure preferable sensitivity and specificity, but also cost can be saved, subtract
The burden of light patient, moreover it is possible to reduce detection time, have the advantages that quick, accurate, economy, be convenient for clinical promotion and application, certainly
Using wherein 1 marker can also, it is more preferable using 2 marker effects.This kit includes a collection of urine fatty acid metabolism
Analyte detection reagent and consumptive material, the wherein mark that is qualitative and quantitatively using tetracosandioic acid and hexadecandioic acid (hexadecane diacid) of fatty acid metabolism object
Quasi- product, assistant analysis use internal standard A：Creatinine, valine, niacin, thymidine, glutaric acid, L- phenylpropylamines acid, N- acetyl pair
The deuterium mark Isotopic Internal Standard of eight kinds of amino phenols, hippuric acid substances.Internal standard B：The deuterium mark Isotopic Internal Standard of pentadecanoic acid.Internal standard C：Two
The deuterium mark Isotopic Internal Standard of tetradecanoic acid.Other mating reverse chromatograms column (Hypersil for being also useful for UPLC chromatographic isolations
GOLD C18 chromatographic columns, 100mm × 2.1mm, 1.9 μm of grain size), (water containing 0.1% formic acid and contain for the reagent of mobile phase
The acetonitrile of 0.1% formic acid), the reagent (100% ultra-pure water) for extracting fatty acid metabolism object.The value of this kit is
Only need 300 μ l urines, you can the content of detection urine fatty acid metabolism object marker, then idiopathic male is diagnosed by content
Infertility, and be easy to carry out dynamic monitoring and observe therapeutic effect.
Specific kit forms are as follows：
Tetracosandioic acid standard items
Hexadecandioic acid (hexadecane diacid) standard items
Internal standard A：Creatinine, valine, niacin, thymidine, glutaric acid, L- phenylpropylamines acid, N- acetaminophens, horse
The deuterium mark Isotopic Internal Standard of eight kinds of substances of uric acid
Internal standard B：The deuterium mark Isotopic Internal Standard of pentadecanoic acid
Internal standard C：The deuterium mark Isotopic Internal Standard of lignoceric acid
Further, can also contain：
Chromatographic column (Thermo 100mm × 2.1mm, 1.9 μm of grain size, Hypersil GOLD C18 chromatographic columns)
Reagent A (contains 100% methanol)
Reagent B (water containing 0.1% formic acid)
Reagent C (acetonitrile containing 0.1% formic acid)
Reagent D (100% ultra-pure water).
Leading reference
Asiago,V.M.,L.Z.Alvarado,N.Shanaiah,G.A.Gowda,K.Owusu-Sarfo,
R.A.Ballas,and D.Raftery.2010.Early detection of recurrent breast cancer
using metabolite profiling.Cancer Res 70:8309-8318.
Brindle,J.T.,H.Antti,E.Holmes,G.Tranter,J.K.Nicholson,H.W.Bethell,
S.Clarke,P.M.Schofield,E.McKilligin,D.E.Mosedale,and D.J.Grainger.2002.Rapid
and noninvasive diagnosis of the presence and severity of coronary heart
disease using 1H-NMR-based metabonomics.Nat Med 8:1439-1444.
Dunn,W.B.,D.I.Broadhurst,H.J.Atherton,R.Goodacre,and
J.L.Griffin.2011.Systems level studies of mammalian metabolomes:the roles of
mass spectrometry and nuclear magnetic resonance spectroscopy.Chemical
Society reviews 40:387-426.
Glinski,M.,and W.Weckwerth.2006.The role of mass spectrometry in
plant systems biology.Mass spectrometry reviews 25:173-214.
Godin,J.P.,L.B.Fay,and G.Hopfgartner.2007.Liquid chromatography
combined with mass spectrometry for 13C isotopic analysis in life science
research.Mass spectrometry reviews 26:751-774.
Locasale,J.W.,A.R.Grassian,T.Melman,C.A.Lyssiotis,K.R.Mattaini,
A.J.Bass,G.Heffron,C.M.Metallo,T.Muranen,H.Sharfi,A.T.Sasaki,D.Anastasiou,
E.Mullarky,N.I.Vokes,M.Sasaki,R.Beroukhim,G.Stephanopoulos,A.H.Ligon,
M.Meyerson,A.L.Richardson,L.Chin,G.Wagner,J.M.Asara,J.S.Brugge,L.C.Cantley,
and M.G.Vander Heiden.2011.Phosphoglycerate dehydrogenase diverts glycolytic
flux and contributes to oncogenesis.Nat Genet 43:869-874.
Munger,J.,B.D.Bennett,A.Parikh,X.J.Feng,J.McArdle,H.A.Rabitz,T.Shenk,
and J.D.Rabinowitz.2008.Systems-level metabolic flux profiling identifies
fatty acid synthesis as a target for antiviral therapy.Nat Biotechnol 26:
1179-1186.
Nicholson,J.K.,J.Connelly,J.C.Lindon,and E.Holmes.2002.Metabonomics:a
platform for studying drug toxicity and gene function.Nat Rev Drug Discov 1:
153-161.
Soga,T.,M.Sugimoto,M.Honma,M.Mori,K.Igarashi,K.Kashikura,S.Ikeda,
A.Hirayama,T.Yamamoto,H.Yoshida,M.Otsuka,S.Tsuji,Y.Yatomi,T.Sakuragawa,
H.Watanabe,K.Nihei,T.Saito,S.Kawata,H.Suzuki,M.Tomita,and
M.Suematsu.2011.Serum metabolomics reveals gamma-glutamyl dipeptides as
biomarkers for discrimination among different forms of liver disease.Journal
of hepatology55:896-905.
Sreekumar,A.,L.M.Poisson,T.M.Rajendiran,A.P.Khan,Q.Cao,J.Yu,B.Laxman,
R.Mehra,R.J.Lonigro,Y.Li,M.K.Nyati,A.Ahsan,S.Kalyana-Sundaram,B.Han,X.Cao,
J.Byun,G.S.Omenn,D.Ghosh,S.Pennathur,D.C.Alexander,A.Berger,J.R.Shuster,
J.T.Wei,S.Varambally,C.Beecher,and A.M.Chinnaiyan.2009.Metabolomic profiles
delineate potential role for sarcosine in prostate cancer progression.Nature
457:910-914.
Suhre,K.,S.Y.Shin,A.K.Petersen,R.P.Mohney,D.Meredith,B.Wagele,
E.Altmaier,P.Deloukas,J.Erdmann,E.Grundberg,C.J.Hammond,M.H.de Angelis,
G.Kastenmuller,A.Kottgen,F.Kronenberg,M.Mangino,C.Meisinger,T.Meitinger,
H.W.Mewes,M.V.Milburn,C.Prehn,J.Raffler,J.S.Ried,W.Romisch-Margl,N.J.Samani,
K.S.Small,H.E.Wichmann,G.Zhai,T.Illig,T.D.Spector,J.Adamski,N.Soranzo,and
C.Gieger.2011.Human metabolic individuality in biomedical and pharmaceutical
research.Nature 477:54-60.
Wang,J.,P.Alexander,L.Wu,R.Hammer,O.Cleaver,and
S.L.McKnight.2009.Dependence of mouse embryonic stem cells on threonine
catabolism.Science325:435-439.
Wang,T.J.,M.G.Larson,R.S.Vasan,S.Cheng,E.P.Rhee,E.McCabe,G.D.Lewis,
C.S.Fox,P.F.Jacques,C.Fernandez,C.J.O'Donnell,S.A.Carr,V.K.Mootha,J.C.Florez,
A.Souza,O.Melander,C.B.Clish,and R.E.Gerszten.2011a.Metabolite profiles and
the risk of developing diabetes.Nat Med 17:448-453.
Wang,Z.,E.Klipfell,B.J.Bennett,R.Koeth,B.S.Levison,B.Dugar,
A.E.Feldstein,E.B.Britt,X.Fu,Y.M.Chung,Y.Wu,P.Schauer,J.D.Smith,H.Allayee,
W.H.Tang,J.A.DiDonato,A.J.Lusis,and S.L.Hazen.2011b.Gut flora metabolism of
phosphatidylcholine promotes cardiovascular disease.Nature 472:57-63.
Zeng J,Huang X,Zhou L,Tan Y,Hu C,Wang X,Niu J,Wang H,Lin X,Yin
P.Metabolomics Identifies Biomarker Pattern for Early Diagnosis of
Hepatocellular Carcinoma:from Diethylnitrosamine Treated Rats to
Patients.2015.Sci Rep 5:16101.
Zhang,Y.,Y.Dai,J.Wen,W.Zhang,A.Grenz,H.Sun,L.Tao,G.Lu,D.C.Alexander,
M.V.Milburn,L.Carter-Dawson,D.E.Lewis,H.K.Eltzschig,R.E.Kellems,
M.R.Blackburn,H.S.Juneja,and Y.Xia.2011.Detrimental effects of adenosine
signaling in sickle cell disease.Nat Med 17:79-86。
Claims (1)
1. with the relevant urine fatty acid metabolism object marker of idiopathic male infertility prepare idiopathic male infertility diagnosis or
Application in monitoring reagent box, the marker are urine fatty acid metabolism object tetracosandioic acid and hexadecandioic acid (hexadecane diacid).
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