CN106483111B - Application of the pyrene benzothiazole schiff bases on detection silver ion - Google Patents
Application of the pyrene benzothiazole schiff bases on detection silver ion Download PDFInfo
- Publication number
- CN106483111B CN106483111B CN201610850483.3A CN201610850483A CN106483111B CN 106483111 B CN106483111 B CN 106483111B CN 201610850483 A CN201610850483 A CN 201610850483A CN 106483111 B CN106483111 B CN 106483111B
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- CN
- China
- Prior art keywords
- pyrene
- benzothiazole
- solution
- schiff bases
- silver ion
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- QYIVUQDVYDFKMO-YSMPRRRNSA-N CN(c1nc(cccc2)c2[s]1)/N=C\C1c(ccc2ccc3)c4c2c3C=CC4=CC1 Chemical compound CN(c1nc(cccc2)c2[s]1)/N=C\C1c(ccc2ccc3)c4c2c3C=CC4=CC1 QYIVUQDVYDFKMO-YSMPRRRNSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
Abstract
The invention belongs to metal ion detection technical fields, and in particular to application of the pyrene benzothiazole schiff bases on detection silver ion;The present invention detects silver ion using pyrene benzothiazole schiff bases, is applied to detection probe, in aqueous solution specific detection silver ion, and not only anti-interference ability is good, high sensitivity, but also it is low to detect limit.It is not only convenient and efficient relative to traditional method, it is generally also provided with higher selectivity and sensitivity, therefore is highly suitable for the real-time and in situ detection of heavy metal ion.
Description
Technical field
The invention belongs to metal ion detection technical fields, and in particular to pyrene benzothiazole schiff bases is on detection silver ion
Application.
Background technique
Silver ion is often used to sterilize, its compound has in electronics, photograph, imaging and drug industry widely answers
With being in the past considered as nontoxic metallic element.However, report silver ion and nano silver in recent years are to benign bacterium, amphibious
Animal and fish have potential toxicity.In addition, silver ion can make the inactivation of bacteria containing sulfydryl, with the various metabolin knots such as amine, imidazoles
It closes so as to cause various diseases.Silver ion belongs to the metal ion of silencing type, can quenching fluorescence.Therefore, enhanced Ag is designed+
Fluorescence probe has great importance.
The detection method of silver ion generally comprises atomic absorption spectrum and plasma emission spectroscopy (ICP), electrochemistry side
The means such as method, colorimetric method, biology and nano-sensor and fluorescent ion probe.But there is also not for these existing detection techniques
It is enough that convenient and efficient, selectivity and sensitivity be not high, is not easily adapted for the deficiencies of real-time and in situ detection of heavy metal ion.
Summary of the invention
To solve the above-mentioned problems, the purpose of the present invention is to provide a kind of pyrene benzothiazole schiff bases and its in detection silver
Application on ion.
The second object of the present invention is to provide specific method of the pyrene benzothiazole schiff bases on detection silver ion.
The present invention is achieved through the following technical solutions:
Application of the pyrene benzothiazole schiff bases on detection silver ion, the structural formula of the pyrene benzothiazole schiff bases are
Steps are as follows for the specific method of Silver detection for the pyrene benzothiazole schiff bases:
(1) standard curve is made:Silver ion solution, pyrene benzothiazole Schiff aqueous slkali I are first prepared respectively, silver ion is molten
Liquid is added in pyrene benzothiazole Schiff aqueous slkali I, and configuration concentration of silver ions swashs at the serial solution of change of gradient corresponding
It sends out under wavelength, measures the fluorescence intensity of serial pyrene benzothiazole Schiff aqueous slkali, draw fluorescence pattern, record respective fluorescence hair
Penetrate peak;Wherein, in the serial pyrene benzothiazole Schiff aqueous slkali, a copy of it is that the blank that concentration of silver ions is 0 is molten
Liquid, remaining is non-blank-white solution;The fluorescence intensity for recording non-blank-white solution becomes relative to the fluorescence of the fluorescence intensity of blank solution
Change amount is fitted the standard curve for determining change in fluorescence amount and concentration of silver ions.
(2) detection record:Solution to be measured containing silver ion is added to the configured pyrene benzo in step (1)
In thiazole Schiff aqueous slkali I, mixed solution II is obtained, records mixed solution II relative to pyrene benzothiazole Schiff aqueous slkali I
Change in fluorescence amount;The additional amount of solution to be measured is the note so that the fluorescence intensity of pyrene benzothiazole Schiff aqueous slkali reaches maximum value
Record change in fluorescence amount.
(3) it calculates:The concentration of silver ion in solution to be measured is calculated using standard curve.
Preferably, corresponding excitation wavelength is 320~360nm in step (1);More preferable 350nm.
Preferably, the concentration of pyrene benzothiazole Schiff aqueous slkali is 0.5 × 10 in step (1)-5~3 × 10-5mol/L。
Application of the pyrene benzothiazole schiff bases on detection silver ion, is used for silver for the pyrene benzothiazole schiff bases
Ion fluorescence probe.
The present invention using pyrene benzothiazole schiff bases as fluorescence probe, can in aqueous solution specific detection silver from
Son, anti-interference ability is good, high sensitivity, and detection limit is low.It is not only convenient and efficient relative to traditional method, be generally also provided with compared with
High selectivity and sensitivity, therefore it is easily used for the real-time and in situ detection of heavy metal ion.
Detailed description of the invention
Fig. 1 is 1.0 × 10-5In the pyrene benzothiazole Schiff aqueous slkali of mol/L be added different material amount concentration silver from
Sub- fluorogram (excitation wavelength 350nm);
Fig. 2 is 1.0 × 10-5A variety of different common metal ions are added in the pyrene benzothiazole Schiff aqueous slkali of mol/L
(concentration of every metal ion species is all 1.0 × 10 to change in fluorescence figure-5Mol/L, excitation wavelength 350nm);
Fig. 3 is 1.0 × 10-5The pyrene benzothiazole Schiff aqueous slkali of mol/L is added silver ion while being added a variety of different
Change in fluorescence figure (the concentration of silver ions 1.0 × 10 of common metal ion aqueous solution-5Mol/L, other metal ions 5.0 × 10- 5mol/L);
Fig. 4 is standard curve.
Specific embodiment
The present invention is described in further detail With reference to embodiment, to help those skilled in the art's reason
The solution present invention.
One, pyrene benzothiazole schiff bases identifies silver ion specific selection:
Compound concentration is 1.0 × 10-5The concentrated solution of silver ion is gradually added dropwise in the pyrene benzothiazole Schiff aqueous slkali of mol/L
(concentration 5mmol/L) so that in solution the concentration of silver ion be respectively 0.0,0.5,1,1.5,2,3,4,5,6,7,8,9,10,
12 μm of ol/L measure their fluorescence spectrum using 350nm as excitation wavelength, and fluorescence spectra is as shown in Figure 1.It can be with from Fig. 1
See, with the continuous increase of concentration of silver ions, the fluorescent emission peak intensity at 382nm is gradually increased.Wherein pyrene benzo thiophene
The concentration of azoles Schiff aqueous slkali can be 0.5 × 10-5~3 × 10-5Mol/L, for ease of calculation preferably 1.0 × 10-5mol/L;
Same excitation wavelength is that any one of 320~360nm can be carried out, and the preferred 350nm of the present embodiment, other are no longer superfluous
It states.
Other metal ions, including alkali metal, alkaline-earth metal, transition metal and heavy metal are tested by above-mentioned same step
Ion.The result shows that pyrene benzothiazole schiff bases does not have recognition reaction, the fluorescent emission intensity base of solution to other metal ions
Do not change in sheet.Take various concentration of metal ions be 10 μm of ol/L when fluorescent emission intensity make Fig. 2.
As seen from Figure 2, other metal ions to the fluorescent emission property of pyrene benzothiazole Schiff aqueous slkali substantially
Do not change, only silver ion significantly increases the fluorescence of pyrene benzothiazole Schiff aqueous slkali, shows to silver ion very
High Selective recognition effect.
Two, the detection of concentration of silver ions:
(1) standard curve is made:The silver ion solution of normal concentration (5mmol/L) is added to pyrene benzothiazole schiff bases
(concentration is 1.0 × 10 to solution I-5Mol/L in), configuration concentration of silver ions at change of gradient pyrene benzothiazole Schiff aqueous slkali,
(the change in fluorescence amount is referred to containing silver the change in fluorescence amount of record concentration of silver ions and pyrene benzothiazole Schiff aqueous slkali
The pyrene benzothiazole Schiff aqueous slkali of ion becomes relative to the fluorescence intensity of the pyrene benzothiazole Schiff aqueous slkali of not silver ion
Change value), standard curve of the production concentration of silver ions to change in fluorescence amount;
Wherein,
(2) solution to be measured containing silver ion is added in pyrene benzothiazole Schiff aqueous slkali I again, obtains solution II,
Change in fluorescence amount of the recording solution II relative to solution I;
(3) concentration of silver ion in solution to be measured is calculated using standard curve.
Above-described embodiment, only presently preferred embodiments of the present invention, is not intended to limit the invention practical range, therefore all with this
The equivalent change or modification that feature described in invention claim and principle are done should all be included in scope of the invention as claimed
Within.
Claims (5)
1. application of the pyrene benzothiazole schiff bases on detection silver ion, it is characterised in that:The pyrene benzothiazole schiff bases
Structural formula is
Steps are as follows for the specific method of Silver detection for the pyrene benzothiazole schiff bases,
(1) standard curve is made:First respectively prepare silver ion solution, pyrene benzothiazole schiff bases solution I, then by it is described silver from
Sub- solution is added in the pyrene benzothiazole schiff bases solution I, under corresponding excitation wavelength, records silver in mixed solution
The change in fluorescence amount of ion concentration and solution, standard curve of the production concentration of silver ions to change in fluorescence amount;Wherein, the silver of configuration
Ion concentration is at change of gradient;
(2) detection record:Solution to be measured containing silver ion is added to the configured pyrene benzothiazole in step (1)
In schiff bases solution I, mixed solution I I is obtained, records fluorescence of the mixed solution I I relative to pyrene benzothiazole schiff bases solution I
Variable quantity;
(3) it calculates:The concentration of silver ion in solution to be measured is calculated using standard curve.
2. application of the pyrene benzothiazole schiff bases as described in claim 1 on detection silver ion, it is characterised in that:Step (1)
In corresponding excitation wavelength be 320~360nm.
3. application of the pyrene benzothiazole schiff bases as described in claim 1 on detection silver ion, it is characterised in that:Step (1)
The concentration of middle pyrene benzothiazole schiff bases solution I is 0.5 × 10-5~3 × 10-5mol/L。
4. application of the pyrene benzothiazole schiff bases as described in claim 1 on detection silver ion, it is characterised in that:Step (2)
Solution to be measured containing silver ion specifically, is added to the pyrene benzothiazole schiff bases solution I of known concentration by middle detection record
In, the additional amount of solution to be measured is so that the fluorescence intensity of pyrene benzothiazole Schiff aqueous slkali reaches maximum value, and record fluorescence becomes
Change amount.
5. application of the pyrene benzothiazole schiff bases as described in claim 1 on detection silver ion, it is characterised in that:By the pyrene
Benzothiazole schiff bases is used for silver ion fluorescence probe.
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5439828A (en) * | 1987-07-31 | 1995-08-08 | Alliedsignal Inc. | Fluorgenic and chomogenic three-dimensional ionophores as selective reagents for detecting ions in biological fluids |
CN104402827A (en) * | 2014-10-23 | 2015-03-11 | 华南师范大学 | Preparation method and application of 2-(1-pyrenyl)-benzimidazole |
-
2016
- 2016-09-26 CN CN201610850483.3A patent/CN106483111B/en not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5439828A (en) * | 1987-07-31 | 1995-08-08 | Alliedsignal Inc. | Fluorgenic and chomogenic three-dimensional ionophores as selective reagents for detecting ions in biological fluids |
CN104402827A (en) * | 2014-10-23 | 2015-03-11 | 华南师范大学 | Preparation method and application of 2-(1-pyrenyl)-benzimidazole |
Non-Patent Citations (2)
Title |
---|
一个基于苯并噻唑的铜离子荧光探针的合成、晶体结构和光谱性质;范方禄 等;《无机化学学报》;20150331;第31卷(第3期);548-554 * |
一种应用于活细胞中检测Hg(II)的苯并噻唑类荧光探针;焦元红 等;《无机化学学报》;20150228;第31卷(第2期);361-368 * |
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