CN106483005B - A kind of mounting method quantifying sample identification for phytoplankton, protozoan - Google Patents
A kind of mounting method quantifying sample identification for phytoplankton, protozoan Download PDFInfo
- Publication number
- CN106483005B CN106483005B CN201610947661.4A CN201610947661A CN106483005B CN 106483005 B CN106483005 B CN 106483005B CN 201610947661 A CN201610947661 A CN 201610947661A CN 106483005 B CN106483005 B CN 106483005B
- Authority
- CN
- China
- Prior art keywords
- sample
- glycerol
- coverslip
- paper
- phytoplankton
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of mounting methods that phytoplankton, protozoan quantify sample identification, and step is: A, taking paper, disseminate glycerol, be placed on glass slide, repeat 2-3 times, glycerol is uniformly covered with coverslip, the scraps of paper remaining glycerol dip dyeing before using；B, quantitative phytoplankton, Protozoan specimen dilute after being concentrated in water body, take dilution in the counting frame of 0.1ml, shake glass slide, sample is allowed to be laid in counting frame；C, it takes coverslip to carry out cover plate to sample along 45 degree of directions, the air in counting frame is gradually discharged；D, it is sealed with the scraps of paper that the first step has disseminated glycerol along the surrounding of coverslip；E, at room temperature, after the completion of to sample count, sample there are no bubble appearance in counting frame, which improves mounting effect.Method is easy to operate, low in cost, reusable, will not pollute microscopical object lens, while the significant effect of mounting, improve work efficiency, improve the accuracy of sample count.
Description
Technical field
The invention belongs to water ecology research fields, are more particularly to one kind for phytoplankton, protozoan and quantify sample
Fixed mounting method is judged, phytoplankton, protozoan quantify sample suitable for the water bodys such as reservoir, lake, river, pond
Pre-treatment when identification can save the time of repetition film-making, the accuracy to develop skill.
Background technique
Phytoplankton is the photoautotroph that life of swimming is sought in water body, is the producer of water body.Their individual
Service life is usually very short, from several hours to tens days, can breed many generations within a growth period.Since individual is smaller, float
Trip plant is very rapid to the response of environmental change, is one of the important indicator of water eutrophication monitoring.Protozoan conduct
The primary consumer of water body is that one kind comes in every shape, the wide unicellular organism of distributed pole, they aquatile substance circulation and
Particularly important role is played in energy flow, has the function of forming a connecting link.Therefore, phytoplankton and protozoan are wide
It is applied in water environment monitoring and water eutrophication evaluation generally, the instruction that numerous species are also considered as water pollution is raw
Object and utilized extensively.Estimate that the biomass in its water body is common by phytoplankton, protozoic quantitative analysis
Monitoring means.
When carrying out quantitative analysis to phytoplankton and protozoan, due in water body they biomass it is larger, identification
Usually require the long period.Most workers can be diluted sample in counting process, however sample must be when effective
Interior completion counts, and measurement error caused by making to occur in counting frame bubble otherwise will be evaporated because of moisture, it is accurate to influence to count
Property.Based on such a case, mounting measure is taken to coverslip, delaying sample the time of bubble occur is urgently to be solved ask
Topic, in particular in beginner.
Summary of the invention
In order to solve the above-mentioned problems of the prior art, the purpose of the invention is to provide a kind of for plant of swimming
Object, protozoan quantify the mounting method of sample identification, and the mounting method is easy to operate, and glycerol consumption is few, low in cost, can
Recycling, will not pollute microscopical object lens, while the significant effect of mounting, improve work efficiency, mentioning to a certain degree
The accuracy of high sample count.
In order to achieve the above purpose, the invention adopts the following technical scheme:
A kind of phytoplankton, protozoan quantify the mounting method of sample identification, the steps include:
1, take the A4 paper of 80g, the paper have the characteristics that hardness it is big, it is thin, be easy to absorb glycerol.It is cut with scissors
For the paper slip of long 26mm wide 2mm, glycerol (chemical name: glycerine, the purchase of Wuhan Li Borui Biotechnology Co., Ltd) is disseminated,
It is placed on glass slide, repeats 2-3 times, so under gravity, glycerol gradually can uniformly be covered with coverslip, after
The scraps of paper can the remaining glycerol dip dyeing before.Immerged time is unsuitable too long (generally 3-4min), can extend paper in this way
The service life of piece；The amount of dip dyeing excessively (usually 1-3ml) otherwise can should not pollute coverslip, influence the sight under microscope
Examine effect.
2, by (lake, pond etc.) concentration in water body, (method for concentration: sample is stood supernatant after 24-48h with enrichment facility
Liquid pumps) afterwards quantitative phytoplankton, Protozoan specimen add sterilizing distilled water to dilute 10-100 times, take 0.1ml dilution in
In the counting frame of 0.1ml (ZH7116 type plankton counting chamber), glass slide is shaked gently, sample is allowed to be laid in counting frame.
The dilution is exactly that the concentrate solution of applicant's detection adds sterilizing distilled water to reduce the solution obtained after concentration as its name suggests.
Note: being mixture, in daily actual experiment operation, (goes out in each grid in counting frame if concentrating sample concentration is not high
Existing different phytoplankton number is 2-3) it does not need then to dilute, if concentration is too high (being more than above-mentioned quantity), need therefore
The lower dilution of concentration is obtained using the diluted mode of distilled water of sterilizing is added, to preferably test.In summary: all
It is by increasing solvent come the obtained solution of method for reducing concentration is all dilution.
3, coverslip is taken to carry out cover plate (Fig. 3) to sample along 45 degree of directions, the sky in counting frame can be gradually discharged in this method
Gas plays optimal mounting effect.
4, with the first step disseminated the scraps of paper of a small amount of glycerol (0.5-1.0ml) carry out along the surrounding of coverslip it is close
Envelope.The character wherein sealed is as shown in Figure 1, accomplish perfect docking with the position of the tweezers adjustment scraps of paper, to guarantee optimal sealing
Effect.
5, under the conditions of room temperature (20-25 DEG C, same as below), to (3-5h) after the completion of sample count, sample in counting frame
Product have had not yet to see bubble appearance, have sealed.Show that the mounting method is remarkably improved mounting effect, avoids repeating film-making, improve
Working efficiency.
Compared with prior art, the present invention having the following advantages that and effect:
1) operability of this method is simple, and material requested is simple (A4 paper, glycerol), and can reuse, at low cost
It is honest and clean.
2) this method avoids the waste of glycerol and the pollution to object lens, extends with the paper for immersing glycerol come mounting
Microscopical service life.
3) this method mounting effect is obvious, avoids the evaporation of moisture in sample, saves the time for repeating film-making, improves work
Make efficiency, also improves the accuracy of counting.
In order to verify the effect of such method, applicant by the sample of not mounting and in this manner the sample of mounting into
It has gone and has compared, compared the time for occurring bubble in counting frame.Experiment is carried out in a preferable laboratory of sealing effect, is used
Airconditioning control temperature, first set temperature are tested after temperature is stablized.Experimental result is as follows:
Experimental result is shown, within the temperature range of air-conditioning can be arranged, time of such mounting method than commonsense method
Extend 5 hours or more, extremely significant improves mounting effect.
Detailed description of the invention
Fig. 1 is a kind of phytoplankton, protozoan Quantitative measurement mounting schematic device.
Fig. 2 is a kind of scraps of paper schematic diagram of mounting.
The scraps of paper are placed in glycerol and are disseminated, are then placed on glass slide again.
Fig. 3 is the demonstration graph that a kind of 0.1ml counting frame carries out mounting.
Wherein: 1- plankton counting chamber, 2- coverslip, 3- paper slip, 4- glass slide.
Specific embodiment
Embodiment 1:
The present invention will now be described in detail with reference to the accompanying drawings.
It is complete the present invention provides a kind of novel mounting method for quantifying sample identification for phytoplankton, protozoan
Whole structure is as shown in Figure 1,0.1ml ZH7116 type plankton counting chamber 1 in figure, coverslip 2, the paper slip 3 of long 26mm wide 2mm,
Glass slide 4.The length and width of paper slip could be matched, play optimal close with coverslip 2 in this way depending on the size of counting frame
Seal effect.
A kind of phytoplankton, protozoan quantify the mounting method of sample identification, the steps include:
1, the paper slip for being long 26mm wide 2mm by the A4 sheet trimming of 80g with scissors is disseminated glycerol (1.5ml), is placed in load
It on slide 4, is repeated 3 times, so under gravity, glycerol gradually can uniformly be covered with coverslip 2, and later paper slip 3 is equal
It can the remaining glycerol dip dyeing before.The amounts of glycerol of dip dyeing should not be excessive, can excessively pollute coverslip 4, influence under microscope
Observing effect.Immerged time is 3min, unsuitable too long, avoids the service life of loss paper slip 3；
2, Pelteobagrus fulvidraco master is supported to the phytoplankton (based on Chlorophyta, Bacillariophyta and Euglenophyta), primary being concentrated in pond
The distilled water that quantitative sample sterilizing is concentrated in animal (based on infusorian) dilutes 20 times, takes 0.1ml dilution in 0.1ml's
In counting frame, glass slide 4 is shaked gently, sample is allowed to be laid in plankton counting chamber 1.
3, coverslip 2 is taken to carry out cover plate (Fig. 3) to sample along 45 degree of directions, planktonic organism counting can be gradually discharged in this method
Air in frame 1 plays optimal mounting effect.
4, the paper slip 3 for a small amount of glycerol of dip dyeing having been had been prepared for the first step is sealed along the surrounding of coverslip 2.Its
The character of middle sealing is as shown in Figure 1, accomplish perfect docking with the position of the tweezers adjustment scraps of paper, to guarantee optimal sealing effect.
5, at room temperature (23 DEG C), to (4h) after the completion of sample count, sample has had not yet to see bubble and has gone out in counting frame
It is existing, it has sealed, has shown that the mounting method is remarkably improved mounting effect, avoided repeating film-making, improve work efficiency.
Claims (1)
1. a kind of phytoplankton, protozoan quantify the mounting method of sample identification, the steps include:
A, A4 paper is taken, the paper slip of long 26mm wide 2mm is cut to scissors, glycerol is disseminated, is placed on glass slide, is repeated
2-3 times, glycerol is uniformly covered with coverslip, the scraps of paper remaining glycerol dip dyeing, immerged time 3-4min before using；
B, quantitative phytoplankton, Protozoan specimen add sterilizing distilled water to dilute 10-100 times after being concentrated in water body, take
0.1ml dilution shakes glass slide, sample is allowed to be laid in counting frame in the counting frame of 0.1ml, the dilution it is dense
Contracting solution adds sterilizing distilled water to reduce the solution obtained after concentration；
C, it takes coverslip to carry out cover plate to sample along 45 degree of directions, the air in counting frame is gradually discharged；
D, it is sealed with the scraps of paper that the first step has disseminated glycerol 0.5-1.0ml along the surrounding of coverslip；
E, at room temperature, after the completion of to sample count, sample there are no bubble appearance in counting frame, seal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610947661.4A CN106483005B (en) | 2016-10-24 | 2016-10-24 | A kind of mounting method quantifying sample identification for phytoplankton, protozoan |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610947661.4A CN106483005B (en) | 2016-10-24 | 2016-10-24 | A kind of mounting method quantifying sample identification for phytoplankton, protozoan |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106483005A CN106483005A (en) | 2017-03-08 |
| CN106483005B true CN106483005B (en) | 2019-04-16 |
Family
ID=58273080
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610947661.4A Expired - Fee Related CN106483005B (en) | 2016-10-24 | 2016-10-24 | A kind of mounting method quantifying sample identification for phytoplankton, protozoan |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106483005B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109001017A (en) * | 2018-10-24 | 2018-12-14 | 清华大学 | The planktonic organism settler for avoiding bubble from interfering |
-
2016
- 2016-10-24 CN CN201610947661.4A patent/CN106483005B/en not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| 浮游动物定性材料的处理方法;沈嘉瑞;《动物学杂志》;19590331;第85左栏最后1段-右栏第1段 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106483005A (en) | 2017-03-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104007093B (en) | A kind of fast quantification calculates the method for the intrinsic drought-resistant ability of plant | |
| CN102618431B (en) | A kind of mass spectrometric closed photo bioreactor device of Kernel-based methods and frustule process of growth monitoring method | |
| CN101738365A (en) | Accelerated test method for simulating atmospheric corrosion of metal materials | |
| CN103913555A (en) | Method for carrying out toxicity analysis on tail water of sewage treatment plant by using caenorhabditis elegans | |
| Millan-Almaraz et al. | Advantages and disadvantages on photosynthesis measurement techniques: A review | |
| CN106483005B (en) | A kind of mounting method quantifying sample identification for phytoplankton, protozoan | |
| CN110863030A (en) | Method for detecting developmental toxicity by using caenorhabditis elegans | |
| Murulidhar et al. | Ecology, distribution and diversity of phytoplankton of Teetha Wetland in Tumakuru district, Karnataka, India | |
| CN101131385B (en) | Rotifer Brachionus plicatilis microkernel preparation technology | |
| Findlay et al. | Protocols for measuring biodiversity: phytoplankton in freshwater | |
| CN103852368A (en) | Mitochondria DNA observation through MTG-DAPI double-staining of semi-thin sections of cucumber pollen | |
| CN103472194B (en) | Method for measuring and calculating threshold concentration of ecological toxic effect of environmental pollutants | |
| CN105510543A (en) | Method for detecting genotoxic agent in water by utilization of Pseudorasbora parva | |
| Aghashariatmadari et al. | Satellite-based monitoring of the algal communities of aras dam reservoir: meteorological dependence analysis and the footprint of COVID-19 pandemic lockdown on the eutrophication status | |
| CN113884621B (en) | Method for quantitatively absorbing and utilizing bicarbonate by plants | |
| CN103308361A (en) | Chromosome slide preparing method | |
| Welschmeyer et al. | A portable, sensitive plankton viability assay for IMO shipboard ballast water compliance testing | |
| CN106018688A (en) | Method for estimating toxicity contribution rate of metal nanoparticle ions and nano effect | |
| Yin et al. | The ovarian cycle of the fish Leptobotia elongata Bleeker, endemic to China | |
| CN106047849A (en) | Immobilized microbial system as well as preparation method thereof and water toxicity detection method | |
| Jawwad et al. | Plankton Diversity in the Coastal Waters at Klumpang Beach, East Nusa Tenggara, Indonesia | |
| Bekku et al. | Midday depression in root respiration of Quercus crispula and Chamaecyparis obtusa: its implication for estimating carbon cycling in forest ecosystems | |
| Hohenegger et al. | Growth of the foraminifer Nummulites venosus in nature and culture | |
| Sajeeb | Abundance and Biodiversity of Phytoplankton in the Halda River during Monsoon and Post Monsoon period | |
| Camargo et al. | The role of limnological variables and habitat complexity in impacted tropical streams as regulatory factors on the flagellate protozoa community |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190416 Termination date: 20201024 |