CN106479876B - The installation method and application method of a kind of connector, transfer device and the transfer device - Google Patents
The installation method and application method of a kind of connector, transfer device and the transfer device Download PDFInfo
- Publication number
- CN106479876B CN106479876B CN201611129904.XA CN201611129904A CN106479876B CN 106479876 B CN106479876 B CN 106479876B CN 201611129904 A CN201611129904 A CN 201611129904A CN 106479876 B CN106479876 B CN 106479876B
- Authority
- CN
- China
- Prior art keywords
- hole
- connector
- diameter
- transfer device
- connector sleeve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0608—Germ cells
- C12N5/0609—Oocytes, oogonia
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Mechanical Engineering (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to the installation method of a kind of connector, transfer device and the transfer device and application methods.The connector includes connector sleeve, connector and filter core；The connector sleeve be equipped with along axially through through-hole；The through-hole includes the first through hole being sequentially communicated from top to bottom, the second through-hole and third through-hole；The connector be equipped with along axially through connecting hole, the connector is set in the third through-hole of the connector sleeve；The filter core is set in the second through-hole of the connector sleeve.The transfer device includes connector, micro-pipe and suction and blowing device.The present invention provides that a kind of structure is simple, easy to use, sealing effect is good and secure attachment device, it can cooperate the micro-pipe of sizes to be operated, and assembling and it is easy to operate, greatly reduce the sample contamination risk and set-up time that existing tool and tool are likely to occur in, simplifies the operating process in the laboratory IVF.
Description
Technical field
The present invention relates to physics disposition and transfer techniques field is carried out to sample, more particularly, to biology, medicine are real
Test the electrical connector that the samples such as ventricular cell carry out physics disposition and transfer, and in particular to a kind of connector, transfer device and should
The installation method and application method of transfer device.
Background technique
Currently, IVF (assisted reproductive technology) use for laboratory in the processing step to egg mother cell and gamete usually have with
Under it is several:
The acquisition of egg mother cell: several ovum-crown- mound compound after oocyte retrieval, in ovarian follicle night containing patient
Product, and subsidiary some tissues, blood clot etc. need to identify and clean under the microscope as early as possible egg mother cell and are transferred into ovum training
In nutrient solution or fertilization culture solution.The ovum tool that picks for needing to use diameter 120-180um diameter during this comes to ovum mother carefully
Born of the same parents clean, and the operating procedure of corresponding culture solution is transferred to after cleaning.The laboratory IVF is come usually using pasteur pipet at present
It completes.Since pasteur pipet minimum diameter is 1 millimeter, so needing carrying out preoperative, the laboratory operation people of egg mother cell acquisition
Member need pasteur pipet alcolhol burner is heated in advance and is drawn for the glass capillary of diameter 120-180um diameter it is spare.
Egg mother cell removes granular cell: conventional IVF fertilization observes fertilization result and carries out ooecium matter intracytoplasmic sperm injection
(ICSI) it needs to remove the ovarian cumulus and corona radiata granular cell that oocyte peripheral is wrapped in when, makes egg mother cell sufficiently " exposed "
Fertilization result could be observed out and carries out microinjection.The glass capillary of diameter 120-180um diameter is needed during this
Or the stripping ovum needle blow of finished product beats egg mother cell/fertilized eggs until the granular cell for being wrapped in egg mother cell or fertilized eggs periphery is de-
It falls.
The transfer of egg mother cell or fertilized eggs: in entire IVF incubation, be often related to egg mother cell/by
The transfer of smart ovum is transferred to another culture vessel from a culture vessel.Because embryo is during fertilization and follow-up cultivation,
The composition demand of culture solution is different, so needing for the embryonic cell of different phase to be put into corresponding culture solution, is reached
To the requirement of embryonic development.Both it had needed to carry out embryo into multiple transfer.Such as it needs to be fertilized after observing successful fertilization ovum
Ovum, which is transferred to Embryo Culture ware, to be continued to cultivate；The egg mother cell for stripping granular cell is transferred to micromanipulation ware and carries out ooecium matter
Intracytoplasmic sperm injection (ICSI) etc. is required with the glass capillary of diameter 120-180um during these transfers come to ovum
Mother cell or fertilized eggs are moved.)
Currently, the processing and transfer of egg mother cell and fertilized eggs are usually required to use a kind IVF or 2 kinds of tools have come
At usually by firing the pasteur pipet drawn and attenuated and finished product stripping ovum needle.The former for laboratory operation personnel draw in advance it is spare,
The method there may be the problem of be: 1, operator needs long time to complete the work of preparation, including draws and connect
It connects and the rubber head of suction is provided.2, the process for drawing alternate processes and installing rubber head has contaminated risk, increases and intersects dirt
The probability of dye.And shift using finished product stripping ovum needle that there are the following problems: 1, finished product shell ovum needle need to be loaded in it is special
The operation for carrying out next step on ovum needle handle is shelled, needs to expend certain loading time, ease of use is bad.2, finished product shells ovum needle
Diameter it is relatively fixed, such as patient's ovum or embryo is excessive too small can not all operate well.In the prior art, processing is thin
The diameter of born of the same parents or embryo need 80-180um, regardless of requiring reprocess to incite somebody to action using Pasteur's moral suction pipe or glass capillary
Lumen, which is drawn into suitable inner diameter and reconnects corresponding rubber head, to be operated, and connection procedure is relatively time-consuming, complicated (need to by hand by bar
Family name's moral suction pipe or glass capillary connection rubber head or other fastening means).
Summary of the invention
For overcome the deficiencies in the prior art, the first purpose of this invention is to provide that a kind of structure is simple, user
Just, sealing effect is good and secure attachment device, it can cooperate the micro-pipe of sizes to be operated, and assembles and operation side
Just, greatly reduce the sample contamination risk and set-up time that existing tool and tool are likely to occur in, simplify the laboratory IVF
Operating process.
Second object of the present invention is to provide a kind of transfer device using above-mentioned connector.
Third object of the present invention is to provide a kind of installation method of above-mentioned transfer device.
Fourth object of the present invention is to provide a kind of application method of above-mentioned transfer device.
To solve the above problems, technical solution used by the first purpose of this invention is as follows:
A kind of connector, which is characterized in that including connector sleeve, connector and filter core；The connector sleeve is equipped with and passes through along axial direction
Logical through-hole；The through-hole includes the first through hole being sequentially communicated from top to bottom, the second through-hole and third through-hole；The connector
Equipped with along axially through connecting hole, the connector is set in the third through-hole of the connector sleeve；The filter core is set to
In second through-hole of the connector sleeve.
Preferably, the first through hole is the first through hole with internal conical surface, the diameter of the first through hole is from upper
It is gradually reduced down；Second through-hole is the second through-hole with internal conical surface, and the diameter of second through-hole is from top to bottom
It is gradually reduced；The diameter at the maximum gauge end of second through-hole is less than or equal to the straight of the minimum diameter end of the first through hole
Diameter.
Preferably, the outer surface of the filter core is outer conical surface, the outer conical surface of the filter core and second through-hole
Internal conical surface between form cone match.
Preferably, the third through-hole is the third through-hole with internal conical surface, the diameter of the third through-hole is from upper
It is gradually reduced down；The diameter at the maximum gauge end of the third through-hole is less than the straight of the minimum diameter end of second through-hole
Diameter.
Preferably, the outer surface of the connector is outer conical surface, the diameter of the connector gradually subtracts from top to bottom
It is small；The diameter at the maximum gauge end of the connector is less than the diameter at the maximum gauge end of the first through hole, the connector
Minimum diameter end diameter be greater than the first through hole minimum diameter end diameter.
Preferably, the connector is silica gel part, one end of the silica gel part is equipped with funnel-form lead-ingroove, facilitates capillary
Glass tube is inserted into connecting hole.
Preferably, the filter core is polyethylene filter core or PP cotton filter element.
To solve the above problems, technical solution used by second object of the present invention is as follows:
A kind of transfer device, which is characterized in that including connector described in first purpose of the invention, micro-pipe and pressure-vaccum dress
It sets；One end of the micro-pipe is plugged in the connecting hole of the connector of the connector, and the other end passes through the connector sleeve
Third through-hole is protruding；The suction and blowing device has the connector that can be plugged in the first through hole of the connector sleeve.
Preferably, the micro-pipe is glass capillary or pasteur pipet.
Preferably, the suction and blowing device is syringe, rubber head or air bag.
To solve the above problems, technical solution used by third object of the present invention is as follows:
A kind of installation method of transfer device, which comprises the following steps:
1) glass capillary is drawn according to different inner diameters, by the biggish one end insertion connector of the caliber of glass capillary
In connecting hole；
2) connector for connecting glass capillary is inserted by the top end opening of the first through hole of connector sleeve, so that connection
Part is inserted into third through-hole, and makes third through-hole of the lesser one end of the caliber of glass capillary across the connector sleeve
It is protruding；
3) filter core is inserted by the top end opening of the first through hole of connector sleeve so that filter core be connected to connector sleeve it is second logical
At hole, connection component is obtained；
4) it will complete to sterilize spare in the connection component merging vertical packaging box of step 3)；It is packaged in vertical packaging box
At least one connection component；
5) in use, need to only tear the packaging of vertical packaging box, first that the connector of syringe is caught in connector sleeve is led to
Kong Zhong can be used.
Preferably, in step 1), at least 1cm in the insertion connecting hole of glass capillary must not exceed the top of connector
Portion open end.
To solve the above problems, technical solution used by fourth object of the present invention is as follows:
The application method of transfer device, which is characterized in that including in application method one, application method two, application method three
Any one:
Application method one: egg mother cell transfer includes the following steps:
A, the liquor folliculi comprising egg mother cell is poured into culture dish, looks for egg mother cell under the microscope, measure egg mother cell
Diameter；
B, the transfer device for selecting the diameter of internal diameter and egg mother cell to match, is caught in connector sleeve for the connector of syringe
First through hole in, by push and pull transfer device syringe egg mother cell is blown and beaten and is cleaned；
C, in the egg mother cell sucking glass capillary finished cleaning, it is transferred quickly to the culture vessel that side prepares
In；
Application method two: granular cell removing includes the following steps:
D, egg mother cell to be processed is placed in suitable vessels and is placed under microscope, measure egg mother cell diameter；
E, the transfer device for selecting the diameter of internal diameter and egg mother cell to match, is caught in connector sleeve for the connector of syringe
First through hole in, by push and pull transfer device syringe egg mother cell is blown and beaten, by capillary glass bore squeeze
The granular cell of oocyte peripheral is pressed, until granular cell removing finishes；
F, the egg mother cell being disposed is sucked in glass capillary, is transferred quickly to the next step prepared operation training
It supports in vessel；
Application method three: cell transfer or embryo transfer include the following steps:
G, cell to be transferred or embryo are placed and measures diameter under microscope；
H, the transfer device that selection internal diameter and cell to be transferred or the diameter of embryo match, by the connector of syringe
It is caught in the first through hole of connector sleeve, cell or embryo is sucked in glass capillary, are transferred quickly to what another was prepared
In culture vessel.
Compared with prior art, the beneficial effects of the present invention are:
1, the present invention includes connector sleeve, connector and filter core；The connector sleeve be equipped with along axially through through-hole；It is described logical
Hole includes the first through hole being sequentially communicated from top to bottom, the second through-hole and third through-hole；The connector be equipped with along axially through
Connecting hole, the connector is set in the third through-hole of the connector sleeve；The filter core is set to the of the connector sleeve
In two through-holes.In actual use, the first through hole of the connector sleeve with suction and blowing device for connecting；The connecting hole is used for
It is connect with micro-pipe.Suction and blowing device and micro-pipe are attached by the connector, and when use only needs hand syringes by syringe nozzle card
The present apparatus for entering to have connected different-diameter micro-pipe can use at once.Present invention mainly solves atomic small sample is carried out
Atomic processing and transfer operation demand connects " blowing, inhale " power of syringe or the offer of rubber head to even by connector top
It connects the cell that device other end sample to be processed carries out repeatedly to squeeze, to reach the granular cell function around removing egg mother cell
Can, and egg mother cell or embryo are sucked in glass capillary, the forwarding function of another vessel is transferred to from a vessel.
Therefore, that the present invention provides a kind of structures is simple, easy to use, sealing effect is good and secure attachment device, it can cooperate more
The micro-pipe of kind of size is operated, and is assembled and easy to operate, greatly reduces existing tool and tool is likely to occur in
Sample contamination risk and set-up time simplify the operating process in the laboratory IVF.
2, connector of the invention is the integrally formed silica gel part being wrapped in outside glass capillary wide at the top and narrow at the bottom, inner hole
Diameter is slightly less than capillary glass pipe outside diameter, and reasonable taper and hardness Design make it tightly wrap glass capillary, is capillary
Connection between glass tube and silica gel part keeps sealing.It because the tube wall of glass capillary is very thin, is easily broken, so silica gel part
Equipped with funnel-form lead-ingroove, glass capillary is facilitated to be inserted into.The outer diameter of silica gel part and the internal diameter of connector sleeve are consistent, by reasonable
The setting of silica gel hardness, so that silica gel part is close to connector sleeve inner wall when being inserted into connector sleeve, realization connect holding sealing with connector sleeve, together
When be pressed further by silica gel part internal diameter so that the connection of glass capillary and silica gel part seals, by the inside and outside extruding of silica gel part,
Realize the sealing of whole device connection.
3, connector sleeve of the invention is wide at the top and narrow at the bottom, and open end internal diameter is matched integrally formed with conventional syringe connector
Connector realizes sealing by the extruding to silica gel part when being connected to the silica gel part insertion connector sleeve of glass capillary.Material can
The plastics for selecting hardness suitable.Connector sleeve need to meet the silica gel part of insertion, the filter core of insertion, the connector of the syringe of insertion this
The length and taper of three.
4, filter core of the invention is the filter core for meeting the thickness of connector sleeve internal diameter, and filter core is that polyethylene filter core or PP cotton filter
Core eliminates sample cross infection that may be present.
Detailed description of the invention
Fig. 1 is the separate structure schematic diagram of connector described in the embodiment of the present invention 1.
Fig. 2 is the cut-away view of connector described in the embodiment of the present invention 1.
Fig. 3 is the separate structure schematic diagram of transfer device described in the embodiment of the present invention 1.
Fig. 4 is the cut-away view of transfer device described in the embodiment of the present invention 1.
Wherein, 10, connector；11, connector sleeve；111, first through hole；112, the second through-hole；113, third through-hole；12, even
Fitting；121, connecting hole；122, funnel-form lead-ingroove；13, filter core；20, micro-pipe；30, suction and blowing device.
Specific embodiment
Embodiment 1:
Referring to Fig.1-2, a kind of connector 10 described in the present embodiment, including connector sleeve 11, connector 12 and filter core 13；Institute
State connector sleeve 11 be equipped with along axially through through-hole；The through-hole includes the first through hole 111, second being sequentially communicated from top to bottom
Through-hole 112 and third through-hole 113；The connector 12 be equipped with along axially through connecting hole 121, the connector 12 is set to
In the third through-hole 113 of the connector sleeve 11；The filter core 13 is set in the second through-hole 112 of the connector sleeve 11.
The first through hole is the first through hole with internal conical surface, and the diameter of the first through hole gradually subtracts from top to bottom
It is small；Second through-hole is the second through-hole with internal conical surface, and the diameter of second through-hole is gradually reduced from top to bottom；Institute
State the maximum gauge end of the second through-hole diameter be less than or equal to the first through hole minimum diameter end diameter.
The outer surface of the filter core 13 is outer conical surface, the female cone of the outer conical surface of the filter core and second through-hole
Cone match is formed between face.
The third through-hole is the third through-hole with internal conical surface, and the diameter of the third through-hole gradually subtracts from top to bottom
It is small；The diameter at the maximum gauge end of the third through-hole is less than the diameter at the minimum diameter end of second through-hole.
The outer surface of the connector 12 is outer conical surface, and the diameter of the connector is gradually reduced from top to bottom；It is described
The diameter at the maximum gauge end of connector is less than the diameter at the maximum gauge end of the first through hole, and the minimum of the connector is straight
The diameter at diameter end is greater than the diameter at the minimum diameter end of the first through hole.
The connector 12 is silica gel part, and one end of the silica gel part is equipped with funnel-form lead-ingroove 122, facilitates capillary glass
Pipe is inserted into connecting hole 121.Preferably, the length of the silica gel part is about 15mm, the diameter of connecting hole is slightly less than capillary glass
Pipe outside diameter makes it tightly wrap glass capillary, and the connection between glass capillary and silica gel part keeps sealing.
The filter core 13 is PP cotton filter element.
The length of the connector sleeve is 30.7mm, and the length of the first through hole is 8.7mm, the length of second through-hole
For 3.3mm, the length of the third through-hole is 18.7mm, and the length of the connector is 15mm, and the length of the filter core is
3.3mm.The diameter at the maximum gauge end of second through-hole is 3.6mm, and the diameter at minimum diameter end is 3.2mm.
The taper design of the first through hole and the female Luer (luer connector) of syringe are consistent, it is ensured that with
The connection of syringe seals and close.
The taper design of second through-hole will not be moved during pressure-vaccum to position filter core preferably.
The taper design of the third through-hole is and to ensure that silica gel part can be with to guarantee that silica gel part can smoothly enter into
External connection mechanism matches.Meanwhile silica gel part is squeezed, so that the cooperation between glass capillary and silica gel part accomplishes that gap is mended
It repays, realizes sealing cooperation between the two.
Referring to Fig. 3-4, a kind of transfer device, including the connector 10, micro-pipe 20 and suction and blowing device 30；The micro-pipe
20 one end is plugged in the connecting hole 121 of the connector 12 of the connector 10, and the other end passes through the connector sleeve 11
Third through-hole 113 is protruding；The suction and blowing device 30 have can be plugged in the first through hole 111 of the connector sleeve 11
Connector.The micro-pipe is glass capillary.The suction and blowing device is syringe.The connector and syringe or rubber head connect
It connects, the micro-pipe of other end connection different-diameter forms a closed pipeline and provided by air in the syringe that squeezes or relax
" blowing force or suction ".
A kind of installation method of transfer device, comprising the following steps:
1) glass capillary is drawn according to different inner diameters, by the biggish one end insertion connector of the caliber of glass capillary
In connecting hole；At least 1cm in the insertion connecting hole of glass capillary must not exceed the open top end of connector；
2) connector for connecting glass capillary is inserted by the top end opening of the first through hole of connector sleeve, so that connection
Part is inserted into third through-hole, and makes third through-hole of the lesser one end of the caliber of glass capillary across the connector sleeve
It is protruding；
3) filter core is inserted by the top end opening of the first through hole of connector sleeve so that filter core be connected to connector sleeve it is second logical
At hole, connection component is obtained；
4) it will complete to sterilize spare in the connection component merging vertical packaging box of step 3)；It is packaged in vertical packaging box
At least one connection component；
5) in use, need to only tear the packaging of vertical packaging box, first that the connector of syringe is caught in connector sleeve is led to
Kong Zhong can be used.
The application method of transfer device, including any one in application method one, application method two, application method three:
Application method one: egg mother cell transfer includes the following steps:
A, the liquor folliculi comprising egg mother cell is poured into culture dish, looks for egg mother cell under the microscope, measure egg mother cell
Diameter；
B, the transfer device for selecting the diameter of internal diameter and egg mother cell to match, is caught in connector sleeve for the connector of syringe
First through hole in, by push and pull transfer device syringe egg mother cell is blown and beaten and is cleaned；
C, in the egg mother cell sucking glass capillary finished cleaning, it is transferred quickly to the culture vessel that side prepares
In；
Application method two: granular cell removing includes the following steps:
D, egg mother cell to be processed is placed in suitable vessels and is placed under microscope, measure egg mother cell diameter；
E, the transfer device for selecting the diameter of internal diameter and egg mother cell to match, is caught in connector sleeve for the connector of syringe
First through hole in, by push and pull transfer device syringe egg mother cell is blown and beaten, by capillary glass bore squeeze
The granular cell of oocyte peripheral is pressed, until granular cell removing finishes；
F, the egg mother cell being disposed is sucked in glass capillary, is transferred quickly to the next step prepared operation training
It supports in vessel；
Application method three: cell transfer or embryo transfer include the following steps:
G, cell to be transferred or embryo are placed and measures diameter under microscope；
H, the transfer device that selection internal diameter and cell to be transferred or the diameter of embryo match, by the connector of syringe
It is caught in the first through hole of connector sleeve, cell or embryo is sucked in glass capillary, are transferred quickly to what another was prepared
In culture vessel.
Embodiment 2:
The characteristics of the present embodiment is: the micro-pipe is pasteur pipet.The suction and blowing device is rubber head.Other and implementation
Example 1 is identical.
Embodiment 3:
The characteristics of the present embodiment is: the micro-pipe is pasteur pipet.The suction and blowing device is air bag.Other and embodiment
1 is identical.
Embodiment 4:
The characteristics of the present embodiment is: the filter core is polyethylene filter core.It is other same as Example 1.
The above embodiment is only the preferred embodiment of the present invention, and the scope of protection of the present invention is not limited thereto,
The variation and replacement for any unsubstantiality that those skilled in the art is done on the basis of the present invention belong to institute of the present invention
Claimed range.
Claims (6)
1. a kind of biological sample transfer transfer device, which is characterized in that including connector, micro-pipe and suction and blowing device；
The connector includes connector sleeve, connector and filter core；The connector sleeve be equipped with along axially through through-hole；The through-hole packet
Include the first through hole being sequentially communicated from top to bottom, the second through-hole and third through-hole；The connector be equipped with along axially through company
Hole is connect, the connector is set in the third through-hole of the connector sleeve, and the third through-hole is the third with internal conical surface
The diameter of through-hole, the third through-hole is gradually reduced from top to bottom, and the diameter at the maximum gauge end of the third through-hole is less than institute
State the diameter at the minimum diameter end of the second through-hole；The filter core is set in the second through-hole of the connector sleeve;The connector
One end for silica gel part, the silica gel part is equipped with funnel-form lead-ingroove；The filter core is polyethylene filter core or PP cotton filter element；
The connector sleeve is rigid plastics connector sleeve；
One end of the micro-pipe is plugged in the connecting hole of the connector of the connector, and the other end passes through the connector sleeve
Third through-hole is protruding；The micro-pipe is glass capillary or pasteur pipet；
The suction and blowing device has the connector that can be plugged in the first through hole of the connector sleeve；The suction and blowing device is note
Emitter, rubber head or air bag.
2. transfer device according to claim 1, which is characterized in that the first through hole is first with internal conical surface
The diameter of through-hole, the first through hole is gradually reduced from top to bottom；Second through-hole is the second through-hole with internal conical surface,
The diameter of second through-hole is gradually reduced from top to bottom；The diameter at the maximum gauge end of second through-hole is less than or equal to institute
State the diameter at the minimum diameter end of first through hole.
3. transfer device according to claim 2, which is characterized in that the outer surface of the filter core is outer conical surface, described
Cone match is formed between the outer conical surface of filter core and the internal conical surface of second through-hole.
4. transfer device according to claim 1, which is characterized in that the outer surface of the connector is outer conical surface, institute
The diameter for stating connector is gradually reduced from top to bottom；The diameter at the maximum gauge end of the connector is less than the first through hole
The diameter at maximum gauge end, the diameter at the minimum diameter end of the connector are greater than the straight of the minimum diameter end of the first through hole
Diameter.
5. a kind of installation method of transfer device according to claim 1, which comprises the following steps:
1) glass capillary is drawn according to different inner diameters, by the connection of the biggish one end insertion connector of the caliber of glass capillary
Kong Zhong；
2) connector for connecting glass capillary is inserted by the top end opening of the first through hole of connector sleeve, so that connector is inserted
Enter into third through-hole, and the third through-hole for making the lesser one end of the caliber of glass capillary pass through the connector sleeve is outside
It stretches out；
3) filter core is inserted by the top end opening of the first through hole of connector sleeve, so that filter core is connected to the second through-hole of connector sleeve
Place, obtains connection component；
4) it by the connection component merging vertical packaging box for completing step 3), sterilizes spare；It is packaged at least in vertical packaging box
One connection component；
5) in use, need to only tear the packaging of vertical packaging box, the connector of syringe is caught in the first through hole of connector sleeve,
It can be used.
6. the application method of transfer device according to claim 5, which is characterized in that including application method one, user
Any one in method two, application method three:
Application method one: egg mother cell transfer includes the following steps:
A, the liquor folliculi comprising egg mother cell is poured into culture dish, looks for egg mother cell under the microscope, measurement egg mother cell is straight
Diameter；
B, the connector of syringe is caught in the of connector sleeve by the transfer device for selecting the diameter of internal diameter and egg mother cell to match
In one through-hole, the syringe by pushing and pulling transfer device is blown and beaten and is cleaned to egg mother cell；
C, it in the egg mother cell sucking glass capillary finished cleaning, is transferred quickly in the culture vessel that side prepares；
Application method two: granular cell removing includes the following steps:
D, egg mother cell to be processed is placed in suitable vessels and is placed under microscope, measure egg mother cell diameter；
E, the connector of syringe is caught in the of connector sleeve by the transfer device for selecting the diameter of internal diameter and egg mother cell to match
In one through-hole, the syringe by pushing and pulling transfer device blows and beats egg mother cell, squeezes ovum by capillary glass bore
The granular cell of mother cell periphery, until granular cell removing finishes；
F, the egg mother cell being disposed is sucked in glass capillary, is transferred quickly to the next step prepared operation incubator
In ware；
Application method three: cell transfer or embryo transfer include the following steps:
G, cell to be transferred or embryo are placed and measures diameter under microscope；
H, the transfer device that selection internal diameter and cell to be transferred or the diameter of embryo match, the connector of syringe is caught in
In the first through hole of connector sleeve, cell or embryo are sucked in glass capillary, another culture prepared is transferred quickly to
In vessel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611129904.XA CN106479876B (en) | 2016-12-09 | 2016-12-09 | The installation method and application method of a kind of connector, transfer device and the transfer device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611129904.XA CN106479876B (en) | 2016-12-09 | 2016-12-09 | The installation method and application method of a kind of connector, transfer device and the transfer device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106479876A CN106479876A (en) | 2017-03-08 |
| CN106479876B true CN106479876B (en) | 2019-08-30 |
Family
ID=58274717
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611129904.XA Active CN106479876B (en) | 2016-12-09 | 2016-12-09 | The installation method and application method of a kind of connector, transfer device and the transfer device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106479876B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107299050A (en) * | 2017-08-28 | 2017-10-27 | 厦门昶科生物工程有限公司 | It is a kind of to move the mini gun for taking plaque |
| CN108559700B (en) * | 2018-01-30 | 2021-10-08 | 京东方科技集团股份有限公司 | Cell sheet transfer device and cell sheet processing system |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201061640Y (en) * | 2007-08-08 | 2008-05-21 | 孙东 | Non-needle enclosed transfusion joint |
| CN203169276U (en) * | 2013-01-30 | 2013-09-04 | 湖北华美阳光生物科技有限公司 | Novel egg stripping manipulator used in test-tube baby technology |
| CN203778090U (en) * | 2014-02-16 | 2014-08-20 | 彭建军 | Sucker |
| CN203915106U (en) * | 2012-12-19 | 2014-11-05 | 北京奥凯伟迪生物科技有限公司 | One is utilized synestotic dentistry implant |
| CN204246224U (en) * | 2014-11-24 | 2015-04-08 | 遵义医学院 | A kind of novel stripping egg apparatus |
| CN206266616U (en) * | 2016-12-09 | 2017-06-20 | 广州品知医疗器械有限公司 | A kind of connector, transfer device |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010141320A1 (en) * | 2009-06-01 | 2010-12-09 | Mariposa Biotechnology, Inc. | Device for removing cumulus from oocytes |
-
2016
- 2016-12-09 CN CN201611129904.XA patent/CN106479876B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201061640Y (en) * | 2007-08-08 | 2008-05-21 | 孙东 | Non-needle enclosed transfusion joint |
| CN203915106U (en) * | 2012-12-19 | 2014-11-05 | 北京奥凯伟迪生物科技有限公司 | One is utilized synestotic dentistry implant |
| CN203169276U (en) * | 2013-01-30 | 2013-09-04 | 湖北华美阳光生物科技有限公司 | Novel egg stripping manipulator used in test-tube baby technology |
| CN203778090U (en) * | 2014-02-16 | 2014-08-20 | 彭建军 | Sucker |
| CN204246224U (en) * | 2014-11-24 | 2015-04-08 | 遵义医学院 | A kind of novel stripping egg apparatus |
| CN206266616U (en) * | 2016-12-09 | 2017-06-20 | 广州品知医疗器械有限公司 | A kind of connector, transfer device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106479876A (en) | 2017-03-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6611386B2 (en) | In vitro fertilization egg collection chamber | |
| CN103857346B (en) | For gathering the device of biological sample and corresponding method | |
| US20060040340A1 (en) | Microorganism specimen storage, hydrating, transfer and applicator device | |
| CN106479876B (en) | The installation method and application method of a kind of connector, transfer device and the transfer device | |
| CN105997156A (en) | Sampling swab for full-automatic pretreatment | |
| CN206266616U (en) | A kind of connector, transfer device | |
| KR20170069314A (en) | A container assembly for picking free medical treatment included pipet | |
| CN208532785U (en) | A kind of fast and convenient unicellular picking device | |
| CN108441413B (en) | Microcarrier suspension culture online sampling device and operation method | |
| CN219439371U (en) | Device for removing granulosa cells around ovum or transferring embryo | |
| EP3777731B1 (en) | Oocyte-holding pipette for sperm injection methods without cytoplasmic aspiration | |
| CN206945315U (en) | One kind stool takes inspection device | |
| CN110088264B (en) | Adherent cell culture matrix sampling device | |
| TWI656214B (en) | Method for cleaning the surface of eggs or fertilized eggs | |
| US6838278B2 (en) | Device for cell transfer | |
| CN104120079B (en) | Disposable sample reception and inoculator | |
| CN210237649U (en) | Stem cell culture robot | |
| CN202146319U (en) | Disposable secretion sampler | |
| CN209518052U (en) | A kind of tiny seed disinfection case efficiently, easy | |
| CN208933362U (en) | A kind of cell transfer operation pipe | |
| CN205528761U (en) | Move egg apparatus | |
| CN209260102U (en) | A kind of nucleic acid extraction kit | |
| JP2010172231A (en) | Apparatus for collecting cell or colony and method for collecting cell or colony | |
| CN220201924U (en) | Device for continuous sampling in bioreactor culture process | |
| CN209778837U (en) | ovum needle is picked up to pig's ovum |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP02 | Change in the address of a patent holder | ||
| CP02 | Change in the address of a patent holder |
Address after: 510000 east of the fifth floor, 600-2 Guangshan Second Road, Tianhe District, Guangzhou City, Guangdong Province Patentee after: GUANGZHOU PINZHI MEDICAL EQUIPMENT Co.,Ltd. Address before: 510000 Guangdong city of Guangzhou province Panyu District Xiaoguwei Street Outer Ring Road No. 280 building 105-1 Room Department of Guangdong Pharmaceutical University Patentee before: GUANGZHOU PINZHI MEDICAL EQUIPMENT Co.,Ltd. |