CN106474537A - A kind of method preparing the liquid bandage for War injury - Google Patents
A kind of method preparing the liquid bandage for War injury Download PDFInfo
- Publication number
- CN106474537A CN106474537A CN201610906715.2A CN201610906715A CN106474537A CN 106474537 A CN106474537 A CN 106474537A CN 201610906715 A CN201610906715 A CN 201610906715A CN 106474537 A CN106474537 A CN 106474537A
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- CN
- China
- Prior art keywords
- liquid bandage
- war injury
- method preparing
- war
- gross weight
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0009—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form containing macromolecular materials
- A61L26/0023—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L26/00—Chemical aspects of, or use of materials for, wound dressings or bandages in liquid, gel or powder form
- A61L26/0061—Use of materials characterised by their function or physical properties
- A61L26/008—Hydrogels or hydrocolloids
Abstract
The invention discloses a kind of method preparing the liquid bandage for War injury is it is characterised in that comprise the following steps：Step one：Sulfydryl matter acid derivative, viscosifier, wetting agent, preservative are dissolved in deionized water, form component A；Step 2：Acrylate derivative, preservative are dissolved in deionized water, add borate buffer solution, form component B；Step 3：Component A is ejected into wound surface, then again component B is ejected into wound surface, quickly crosslinked within 10 second time, form the liquid bandage for War injury.The liquid bandage for War injury of present invention preparation has excellent biocompatibility, no skin irritation and cytotoxicity it is adaptable to the War injury such as the less gunshot wound of wound, burn and incised wound.The present invention is easy to make simple, quick, has high practical value.
Description
Technical field
The present invention relates to prepared by a kind of method preparing the liquid bandage for War injury, especially one kind be applied to wound
The method of the liquid bandage of the War injury such as less gunshot wound, burn and incised wound.
Background technology
Interim in modern war, various types of burns increase, and how to be occurred in the form of combined injury, all have more wound
Face.For scars of war, widely used conventional bandages (gauze) are mainly using cotton yarn as base material, and its major function is wrapping
Wound or hemostasis.This conventional bandages is bound up a wound loaded down with trivial details, and will consume about up to ten thousand tons of cotton yarns every year.As conventional bandages
Deformation-common adhesive bandage is by gauze, rubber adhesive and to be attached to the active drug composition two on rubber adhesive
Part forms.Gauze plays support in effect, and has the function of absorbing wound transudate；Rubber adhesive adheres to skin table
Fixation is played in face；Active drug composition plays antibacterial, antiinflammatory, the function of hemostasis.But limited in one's ability due to absorbing sepage, and should
Class tourniquet bandage (gauze) is also subject to certain impact and restriction due to its mechanical performance that limits of its material, also can shadow during use
Sound is attractive in appearance.The greatest problem that the use of conventional bandages is restricted is in wound healing process, and binder is easy to regenerating tissues and occurs
Adhesion, when recovering from injury removing it is easy to cause secondary damage.
It is desirable to hydrogel is liquid when using in a lot of biological medicine applications, but fast after reaching appointed part
Speed forms gel, loses flowability." liquid bandage " overcomes many drawbacks of traditional wound surface protective material, imparts wound shield
Manage new function：As binder, adhesive bandage etc. to irregular wound face or some human body protective capacities of being inconvenient to use poor,
Water-proof breathable properties are bad, helpless to the bacterium infection of wound, use inaesthetic shortcoming.However, " liquid bandage "
For wound care, there is obviously advantage：Ratio, if the shape fully according to wound forms one layer of hydrogel, sticks
Hydrogel thin film can stop blooding, using being easily removed after terminating etc..Up to the present, research worker has investigated various ways in fact
The quick-gelatinizing of existing hydrogel.For example, the unsaturated derivant of water-soluble polyethylene glycol and derivant pass through photo-initiated crosslinking
Prepare gel；The solution of the triblock copolymer of the Polyethylene Glycol of specific composition and polypropylene glycol has the solidifying of temperature change initiation
Gel behavior；Cyanoacrylate polymerizable is cross-linked to form gel, gluing for organizing；Glutaraldehyde cross-linking material of gelatin etc..
In general, there are various defects in these above-mentioned hydrogels, such as poor biocompatibility, poor biodegradation
Performance etc..Quick-gelatinizing typically requires the cross-linking agent of high activity, and these compounds generally have larger toxic and side effects.
Sulfydryl is the naturally occurring functional group with good biocompatibility in vivo.It has good reaction
Activity, at identical conditions its reactivity generally than amino high several orders of magnitude.The crosslinking of sulfydryl may be selected activity relatively
Low bio-compatible cross-linking agent, prepared hydrogel has more preferable biocompatibility.Wallace etc. multi-arm (four arms or
12 arms) Polyethylene Glycol mercapto derivatives are dissolved in the sodium phosphate/sodium carbonate buffer (pH=9.6) of 0.3mol/L, multi-arm (four
Arm or 12 arms) Polyethylene Glycol butanediol succimide activated derivatives are dissolved in the sodium phosphate buffer of 0.0005mol/L
(pH=6.0), it is prepared for hydrogel by mixing above two solution, in wound care art, there is obvious advantage
(Wallace etc., US6,624,245).
From the point of view of the research, patent or the product that have just disclosed at present, will have antibacterial bacteriostatic, promote the functions such as wound healing
Macromole combined by various modes and make the research of hydrogel moist dressing and compare and receive publicity, and before market
Scape is huge.But although this hydrogel functionally meets, on wound surface formed gel have on earth how soon how good, still not
The requirement of emergency care of trauma can be reached.
Content of the invention
The technical problem to be solved is to overcome prior art defect, provides that a kind of prepare can Quick cross-linking
Method for the liquid bandage of War injury.
In order to solve above-mentioned technical problem, the method that the preparation that the present invention provides is used for the liquid bandage of War injury, including
Following steps：
Step one：Sulfydryl matter acid derivative, viscosifier, wetting agent, preservative are dissolved in deionized water, form component A；
Step 2：Acrylate derivative, preservative are dissolved in deionized water, add borate buffer solution, formation group
Divide B；
Step 3：Component A is ejected into wound surface, then again component B is ejected into wound surface, formed for War injury
Liquid bandage.
As improvement, in the liquid bandage of described War injury, described sulfydryl matter acid derivative accounts for gross weight ratio and is：0.5-
5%, described acrylate derivative accounts for gross weight than for 0.5-5%, and described viscosifier account for gross weight than for 3-20%, described guarantor
Humectant accounts for gross weight than for 5-35%, and described preservative accounts for gross weight than for 0.05-1%, and described deionized water accounts for gross weight ratio
For 20-60%.
As improvement, described sulfydryl matter acid derivative is one kind of H-1, H-2 or two kinds.
As improvement, described sulfydryl matter acid derivative at least contains three sulfydryls.
As improvement, described acrylate derivative is tetramethyl acrylate derivative.
As improvement, described acrylate derivative be one kind of PEG-2, PEG-4, H-3 or two kinds and more than.
As improvement, described viscosifier are water soluble starch, Polyvinylpyrrolidone, polyvinyl alcohol, gelatin, carboxymethyl are fine
One or more of dimension element, polyvidone, collagen protein, vegetable polysaccharidess.
As improvement, described wetting agent is one of glycerol, propylene glycol, Polyethylene Glycol, glycerol, Sorbitol or many
Kind.
As improvement, described preservative is parabenses, benzoic acid and its salt, sorbic acid and its salt, Ben Zha
Bromo-amine, one or more of chlorhexidine acetate.
As improvement, the pH value of described borate buffer solution is 9.5-11.0.
The beneficial effects of the present invention is：The biocompatibility of primary raw material matter containing sulfydryl acid derivative used by the present invention
By-product that is good, not producing side reaction and do not produce toxic side effect, each component stability being used is good, consumption is few, one-tenth
This is cheap, easy to use be easily achieved quick-gelatinizing, be easily achieved quick-gelatinizing spray preparation, be suitable for multiple not
The advantages of with medical usage；Blending ingredients A and bis- kinds of solution of component B, can occur gelation within 20 seconds.The present invention is especially suitable
Field emergency field with War injury.
Brief description
Fig. 1 is sulfydryl matter acid derivative of the present invention and hyaluronic acid1H NMR spectra.
Specific embodiment
Below in conjunction with accompanying drawing, the present invention is elaborated.
Embodiment 1
The preparation of the acid derivative H-1 of matter containing sulfydryl：
10 grams of hyaluronic acids are dissolved in 1L distilled water.It is subsequently added 12 grams of dithio dipropyl two hydrazides, be sufficiently stirred for dissolving.
With 0.1mol/L dilute hydrochloric acid, above solution ph is adjusted to 4.75, add 19.2 grams of 1- ethyl -3- (3- dimethylamine propyl)
Carbodiimide hydrochloride, continuing stirring makes it fully dissolve.Reaction 12 hours, and keep reacting solution pH value 4.75.
With the sodium hydroxide solution of 1.0mol/L, the pH value of solution in 1 is adjusted to 7.0, is subsequently adding 50 grams of dithiothreitol, DTTs,
And adjust pH value of solution for 8.5.After normal-temperature reaction 24 hours, the hydrochloric acid conditioning solution pH using 1mol/L is 3.5.Then by sample
Load in bag filter and dialyse (molecular cut off 3500), dialysed 4-5 days using pure water, change a dialysis solution for every eight hours.Finally
Collect the solution in Dialysis tubing, lyophilization obtains white fluffy solid.
Embodiment 2
The preparation of the PEG-2 of polyethyleneglycol derivative containing dimethacrylate：
4g Polyethylene Glycol is added in the dichloromethane of 50mL, adds 1g triethylamine, mixed liquor is cooled to 0 DEG C.Then
The dichloromethane solution of 1g methacrylic chloride is added dropwise in reaction system, reacts continuously stirred 12 hours.By three times two
Chloromethanes dissolving and ether deposition and purification finally give end-product.
Embodiment 3
The preparation of the derivant PEG-4 of polyalkylene glycol acrylate containing tetramethyl：
4g Polyethylene Glycol is added in the dichloromethane of 50mL, adds 1g triethylamine, mixed liquor is cooled to 0 DEG C.Then
The dichloromethane solution of 1g methacrylic chloride is added dropwise in reaction system, reacts continuously stirred 12 hours.Then by sample
Load in bag filter and dialyse (molecular cut off 3500), dialysed 4 days using pure water, change a dialysis solution for every eight hours.Finally receive
Solution in collection Dialysis tubing, lyophilization obtains white fluffy solid.
Embodiment 4
The preparation of acrylate derivative H-3：
The 1,4- dioxane of 50mL water for injection and 75mL is added in the hyaluronic acid aqueous solution of the 1wt% of 100mL；Thereto plus
Enter 3mL glycidyl thiophene acrylate, then stir 24 hours in 50 DEG C of thermostatic container；Add sodium chloride to gained
To solution in, be followed by stirring for, and pour the mixture into and in 800mL ethanol, obtain continuous shape precipitation.Fully rinse institute with ethanol
The H-3 precipitating and obtaining after being dried 1.1g obtaining.
Hyaluronic acid preferred molecular weight 20000 to 3000000, more preferably molecular weight 100000 to 2000000, and more preferably
Molecular weight 200000 to 1200000.
Embodiment 5
Configuration component solution A：By 0.5mL2% (mass ratio) sulfydryl matter acid derivative, 0.5mL15% (mass ratio) water solublity forms sediment
Powder solution, 1mL20 polyvinyl alcohol, 0.5mL propylene glycol is added to standby in 20mL syringe；
Configuration component B solution：Borate buffer solution configuration 2% (mass ratio) acrylate derivative being 9.5 with pH, standby；
Component solution A is sprayed on glass plate, then component B is sprayed on component solution A, it is possible to find after 10s, form one layer of tool
There are certain viscosity and the hydrogel layer of elasticity.
Embodiment 6
The impact to crosslinking time for the pH：
Embodiment 7
The impact to crosslinking time for the concentration (mass ratio) of component A and component B：
1With distilling water dissolution, concentration is 2% (mass ratio)
2With boric acid-sodium hydrate buffer solution (pH=11.0) dissolving.Concentration is A+B after 2% (mass ratio) mixing, and concentration is respectively
1% (mass ratio).
Embodiment 8
The present invention is used for the test of the liquid bandage water retention property of War injury.Test shows, sample 4, No. 5 and No. 9 performances
Go out excellent water retention property.Preferably propylene glycol is as water-retaining agent.
Embodiment 9
The present invention is used for the optimization Test of each component of the liquid bandage of War injury.The experimental condition of preferred sample 8.
The above is only the preferred embodiment of the present invention it is noted that ordinary skill people for the art
For member, some improvement can also be made under the premise without departing from the principles of the invention, these improvement also should be regarded as the present invention's
Protection domain.
Claims (10)
1. a kind of method preparing the liquid bandage for War injury is it is characterised in that comprise the following steps：
Step one：Sulfydryl matter acid derivative, viscosifier, wetting agent, preservative are dissolved in deionized water, form component A；
Step 2：Acrylate derivative, preservative are dissolved in deionized water, add borate buffer solution, formation group
Divide B；
Step 3：Component A is ejected into wound surface, then again component B is ejected into wound surface, formed for War injury
Liquid bandage.
2. the preparation according to claim 1 be used for War injury liquid bandage method it is characterised in that：Described for
In the liquid bandage of War injury, sulfydryl matter acid derivative accounts for gross weight ratio and is：0.5-5%, described acrylate derivative accounts for gross weight
Than being 0.5-5%, described viscosifier account for gross weight ratio for 3-20% to amount, and described wetting agent accounts for gross weight ratio for 5-35%, described anti-corrosion
Agent accounts for gross weight than for 0.5-1%, and described deionized water accounts for gross weight than for 20-60%.
3. the method preparing the liquid bandage for War injury according to claim 1 and 2 it is characterised in that：Described mercapto
Substrate acid derivative is one kind of H-1, H-2 or two kinds.
4. the method preparing the liquid bandage for War injury according to claim 1 and 2 it is characterised in that：Described mercapto
Substrate acid derivative at least contains three sulfydryls.
5. the method preparing the liquid bandage for War injury according to claim 1 and 2 it is characterised in that：Described third
Olefin(e) acid ester derivant is tetramethyl acrylate derivative.
6. the method preparing the liquid bandage for War injury according to claim 1 and 2 it is characterised in that：Described third
Olefin(e) acid ester derivant be one kind of PEG-2, PEG-4, H-3 or two kinds and more than.
7. the method preparing the liquid bandage for War injury according to claim 1 and 2 it is characterised in that：Described increasing
Stick is water soluble starch, Polyvinylpyrrolidone, polyvinyl alcohol, gelatin, carboxymethyl cellulose, polyvidone, collagen protein, plant
One or more of thing polysaccharide.
8. the method preparing the liquid bandage for War injury according to claim 1 and 2 it is characterised in that：Described guarantor
Humectant is one or more of glycerol, propylene glycol, Polyethylene Glycol, glycerol, Sorbitol.
9. the method preparing the liquid bandage for War injury according to claim 1 and 2 it is characterised in that：Described anti-
Rotten agent be parabenses, benzoic acid and its salt, sorbic acid and its salt, this bundle bromo-amine, one kind of chlorhexidine acetate or
Multiple.
10. the preparation according to claim 1 be used for War injury liquid bandage method it is characterised in that：Borate buffer
The pH value of solution is 9.5-11.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610906715.2A CN106474537A (en) | 2016-10-18 | 2016-10-18 | A kind of method preparing the liquid bandage for War injury |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610906715.2A CN106474537A (en) | 2016-10-18 | 2016-10-18 | A kind of method preparing the liquid bandage for War injury |
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| Publication Number | Publication Date |
|---|---|
| CN106474537A true CN106474537A (en) | 2017-03-08 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610906715.2A Pending CN106474537A (en) | 2016-10-18 | 2016-10-18 | A kind of method preparing the liquid bandage for War injury |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110755676A (en) * | 2019-10-21 | 2020-02-07 | 浙江大学 | Composite dressing for promoting wound healing and regeneration and loading traditional Chinese medicine exosomes and preparation method thereof |
| US11446256B1 (en) | 2021-08-20 | 2022-09-20 | Advantice Health, Llc | Aqueous wound healing formulation |
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| CN103732264A (en) * | 2011-04-20 | 2014-04-16 | 卡比兰生物外科公司 | In-situ gel forming compositions |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110755676A (en) * | 2019-10-21 | 2020-02-07 | 浙江大学 | Composite dressing for promoting wound healing and regeneration and loading traditional Chinese medicine exosomes and preparation method thereof |
| CN110755676B (en) * | 2019-10-21 | 2021-06-15 | 浙江大学 | Composite dressing for promoting wound healing and regeneration and loading traditional Chinese medicine exosomes and preparation method thereof |
| US11446256B1 (en) | 2021-08-20 | 2022-09-20 | Advantice Health, Llc | Aqueous wound healing formulation |
| US11844869B2 (en) | 2021-08-20 | 2023-12-19 | Advantice Health, Llc | Aqueous wound healing formulation |
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