CN106474224A - A kind of protective agents of Rana grylia red leg disease and preparation method thereof - Google Patents

Abstract

The invention discloses a kind of protective agents of Rana grylia red leg disease, which is made up of the bulk drug of following masses part proportioning：170 210 parts of rheum officinale, 50 80 parts of the shell of seaear, 190 220 parts of toothed burclover, 180 215 parts of the Radix Astragali, 180 210 parts of myrrh, 80 110 parts of wormwood, 130 165 parts of salvia chinensis, 185 215 parts of cape jasmine, 105 135 parts of wilsonii, 105 140 parts of polygala root, 135 160 parts of the root of herbaceous peony, 120 150 parts of Chinese Fevervine Herb.In the medicine there is no incompatibility in each material combination, can substantially reduce the incidence of Rana grylia red leg disease, have good preventive and therapeutic action to Rana grylia disease, and have no toxic side effect, and using safety, remain nothing hazardous drugs.

Description

A kind of protective agents of Rana grylia red leg disease and preparation method thereof

Technical field

The invention belongs to frog cultural technique field, and in particular to a kind of protective agents of Rana grylia red leg disease and its system Preparation Method.

Background technology

Rana grylia also known as the river frog, the water frog.The U.S. is originated in, the later stage eighties 20th century introduces China, its fine and tender taste, Delicious flavour, is first-class Rana esculenta, has promoted cultivation throughout the country.Rana grylia has that individual big, growth is fast, taste Delicious, protein content is high, is a kind of high-quality and efficient economic cultivation batrachia.As industry competition is fierce, some old raisers Cultural technique old stuff, causes to produce effect lowly, and some can't regain one's original capital, and some cultivation are not stinted using some forbidden drugs, cause frog Internal harmful substance accumulation, so as to cause mortality, also makes cultivation water suffer severe contamination, also ecological around collateral damage Environment.

In breeding process, large-scale cultivation is susceptible to disease to Rana grylia, and is very easy to spread, mainly There is following disease：Tadpole period Common Diseases：Disease, septicemia, enteritis, water mold, ascites, trichodinasis, bubble illness；Become the frog common Disease：Enteritis, red leg disease, infectious liver disease, white-eyes-sickness, bark rot etc., by putting into practice, exploring, Experimental comparison, take to prevent Based on.

Wherein Rana grylia red leg disease, invades wound by bacterium and causes, and sick frog body blacks, or bows and throw oneself on the ground, or water of hiding In, motionless do not ingest, when serious four limbs rot, inflammation, under interior stock and abdomen, there is substantially red point or erythema in skin.The disease often with rotten The diseases such as skin, enterogastritis are concurrent, and infectiousness is exceedingly fast.Once it was found that, the disease frog will be isolated in time.Typically blue or green using Western medicine in prior art Mycin or streptomysin preventing and treating Rana grylia red leg disease, easily cause pharmacological dependence, or impact frog meat, it is therefore necessary to open The protective agents for sending out Rana grylia red leg disease a kind of.

Content of the invention

It is an object of the invention to overcoming the deficiencies in the prior art, a kind of protective agents of Rana grylia red leg disease are provided, In medicine there is no incompatibility in each material combination, can substantially reduce the incidence of Rana grylia red leg disease, red to Rana grylia Leg disease has good preventive and therapeutic action, and has no toxic side effect, and using safety, remains nothing hazardous drugs.

The technical solution adopted for the present invention to solve the technical problems is：

The technical scheme is that：A kind of protective agents of Rana grylia red leg disease, its be by the original of following masses part proportioning Material medicine is made：Rheum officinale 170-210 part, shell of seaear 50-80 part, toothed burclover 190-220 part, Radix Astragali 180-215 part, myrrh 180-210 Part, wormwood 80-110 part, salvia chinensis 130-165 part, cape jasmine 185-215 part, wilsonii 105-135 part, polygala root 105-140 part, white Chinese herbaceous peony 135-160 part, Chinese Fevervine Herb 120-150 part.

Its further preferred mass part proportioning is：Rheum officinale 170-205 part, shell of seaear 50-75 part, toothed burclover 190-215 part, the Radix Astragali 190-215 part, myrrh 185-210 part, wormwood 80-105 part, salvia chinensis 130-160 part, cape jasmine 185-210 part, wilsonii 105- 130 parts, polygala root 110-140 part, root of herbaceous peony 140-160 part, Chinese Fevervine Herb 125-150 part.

Its further preferred mass part proportioning is：Rheum officinale 180-205 part, shell of seaear 50-70 part, toothed burclover 190-210 part, the Radix Astragali 190-210 part, myrrh 190-210 part, wormwood 90-105 part, salvia chinensis 140-160 part, cape jasmine 190-210 part, wilsonii 110- 130 parts, polygala root 120-140 part, root of herbaceous peony 145-160 part, Chinese Fevervine Herb 130-150 part.

Its further preferred mass part proportioning is：Rheum officinale 190-205 part, shell of seaear 55-70 part, toothed burclover 190-205 part, Radix Astragali 195-210 part, myrrh 190-205 part, wormwood 95-105 part, salvia chinensis 140-155 part, cape jasmine 190-205 part, wilsonii 110-125 part, polygala root 120-135 part, root of herbaceous peony 145-155 part, Chinese Fevervine Herb 135-150 part.

Its further preferred mass part proportioning is：Rheum officinale 190-200 part, shell of seaear 60-70 part, toothed burclover 190-200 part, Radix Astragali 200-210 part, myrrh 190-200 part, wormwood 100-105 part, salvia chinensis 150-155 part, cape jasmine 200-205 part, wilsonii 110-120 part, polygala root 130-135 part, root of herbaceous peony 145-150 part, Chinese Fevervine Herb 140-150 part.

Its further preferred mass part proportioning is：200 parts of rheum officinale, 60 parts of the shell of seaear, 200 parts of toothed burclover, 200 parts of the Radix Astragali, 200 parts of myrrh, 100 parts of wormwood, 150 parts of salvia chinensis, 200 parts of cape jasmine, 120 parts of wilsonii, 130 parts of polygala root, 150 parts of the root of herbaceous peony, chicken droppings 140 parts of rattan.

The primary raw material medicine that the present invention is used is described as follows：

Rheum officinale:Rheum officinale is being collectively referred to as the perennial plant of multiple polygonaceae Rheums, and the title of Chinese medicine.Rheum officinale of the present invention Refer to todaiwo,rhizoma.Excavation before the withered or secondary spring germination of autumn Mo cauline leaf.Radicula is removed, crust, valve cutting or section is scraped off, rope is worn into String dries or convection drying, and Chinese herb rhubarb has attacks the effects such as stagnant, clearing away damp-heat, purging intense heat, cool blood, dissolving stasis, removing toxic substances.

The shell of seaear：Shell for Bao Ke animal Haliotis diversicolor, haliotis discus hannai Ino, ear Bao, sheep Bao etc..Also known as natural pearls are female（《Thunder God big gun Process opinion》）, abalone first（《Bencao jizhu》）, nine hole spiral shells（《Japan hanako materia medica》）, climbing groundsel（《Detailed outline》）, very sea certainly, Hai Jue Bright, CONCHA HALIOTIDI, abalone shells, the nine hole shells of seaear（《Pharmacognosy》）, abalone skin（《Shandong Chinese medicine handbook》）, golden clam skin（《In Shandong Herbal medicine》）.There is calming liver and clearing heat, effect of screen is gone in improving eyesight.This product is conventional Chinese medicine, and " Mingyi Bielu " is classified as top grade.Modern commercially available product is divided Determine for light bottom sea, hair bottom sea is certainly determined three kinds with sea.Certainly former animal is haliotis diversicolor Reeve in light bottom sea, and the extra large original animal of determining in hair bottom is that disk is big Bao, sea former animal certainly are sheep Bao.Other title natural pearls are female, abalone first, nine hole spiral shells, climbing groundsel, abalone skin, golden clam skin.

Toothed burclover:It is the address of the people to bur clover on the ground such as Shanghai, granary, Changshu.Bur clover, original name south lucerne vegetables MedicagohispidaGaertn has Ye Sanchi, such as reversed cardioid, and sawtooth is arranged at tip slightly circle or recessed top, and the surface of leaf is in dense Green, stalk are extremely short, and main root is long, multi-branched.Stem is generally vertical, near nothing hair, high 30-100 centimetre.Compound leaf has 3 leaflets, leaflet Obovate or lanceolar, long 1-2 centimetre, wide about 0.5 centimetre, nose-circle, middle rib slightly protrude, and first half leaf has sawtooth, base portion Narrow wedge shape；Stipule narrow lanceolar, full edge.Raceme armpit is given birth to, flower 8-25.Pod spirality, stingless, top has point to expose nozzle；? The production of two province of Jiangsu and Zhejiang Provinces is special many.Toothed burclover is legume, often eat toothed burclover element can balanced human pH-value.

The Radix Astragali：Also known as continuous stilbene.Perennial herb is high 50-100 centimetre.Main root is plump, wooden, normal branch, canescence.Stem Uprightly, top multi-branched, has thin rib, by white pubescence.Perennial herb is high 50-100 centimetre.Originate in Inner Mongol, Shanxi, sweet The ground such as respectful, Heilungkiang.The medicinal history for having more than 2000 year so far of the Radix Astragali, which has enhancing body's immunity, liver protection, profit Urine, anti-aging, resisting stress, step-down and wide antibacterial action.But table is real evil to be contained, and retention of dampness due to stagnation of QI, dyspepsia are stagnated, ulcer from the beginning of Or the real example such as heat toxin Shang Sheng after bursting, and hyperactivity of yang due to yin deficiency person, clothes must be prohibited.

Myrrh：Chinese medicine name, is olive subject plant Chinese violet tree Commiphora myrrha Engl. or Ha Didingshu The dry resin of Commiphora molmol Engl..It is divided into natural myrrh and gum opoponax.It is distributed in Somalia, Ethiopia Russia Than sub- and Arabia Peninsula south and other places.The effect of with scattered stasis pain, detumescence and promoting granulation.It is usually used in chest impediment and cardialgia, gastral cavity pain, Dysmenorrhoea Amenorrhea, postpartum stasis, lump in the abdomen are suffered from abdominal pain, arthralgia due to wind-dampness, traumatic injury, the treatment of the illness such as carbuncle swells sore.

Wormwood：Perennial herb slightly becomes suffruticose, and plant has enhanced aroma.The life of stem list or minority, brown or sallow Brown, base portion slightly lignifying, top Bi matter, and the branch for having minority short, leaf ground paper matter, above by canescence pubescence, base portion Generally nothing pseudostipule or minimum pseudostipule；Upper leaf and bract leaf pinniform are partly split, capitulum ellipse, corolla tubulose or height Pin cup-shaped, outside have gland point, and flower pesticide is narrow linear, the closely isometric or slightly longer than corolla of style and corolla.Achene long avette or Long Circle. The flowering fruit bearing stage 9-10 month.All herbal medicine, have Wen Jing, dry, dissipates cold, hemostasis, anti-inflammatory, relieving asthma, cough-relieving, the effect such as antiabortive, antiallergy. Tarragon is dried and smashs to pieces " moxa ", and moxa stick processed can make the raw material of " ink paste " again for moxa-moxibustion.

Salvia chinensis：Also known as：Dali madder, Yunnan madder, red root, dice piece etc., genus rubia mesh, Rubiaceae draft；Indication： Clearing heat and detoxicating, promoting blood circulation qi-regulating pain relieving.Coerce under distending pain, humid tropics for acute, chronic hepatitis, gastral cavity, mastitis, malignant boil swell.

Cape jasmine：Another name Yellow Fructus Gardeniae, mountain Cape jasmine, white toad, gardenia, are the fruits of madder wort cape jasmine.Genus rubia section, cape jasmine Category, evergreen shrubs；Root, leaf, fruit can all be used as medicine, and have the effect of purging intense heat relieving restlessness, reducing fever and causing diuresis, removing pattogenic heat from the blood and toxic material from the body；Effect：Clearly Lung cough-relieving, cooling blood and hemostasis, purging intense heat, cough with lung heat is cured mainly, is coughed up phlegm, pyogenic infections, control pyreticosis restlessness of asrhenia type and insomnia, jaundice, gonorrhoea, quench one's thirst, mesh Red, pharyngalgia, spit blood, bleeding from five sense organs or subcutaneous tissue, bloody flux, hematuria, pathopyretic ulcer, sprain and swell and ache.《Dictionary of medicinal plant》Described in：Cape jasmine, different name：Wood Red (《This warp》), fresh (《Upper woods is assigned》), ancient wine vessel (《Chinese book》Meng Kang note), more peach (《Do not record》), stand (《Bencao jizhu》), Fructus gardeniae (《Haigoushen》), branch (《Tang materia medica》), little danger son (《Book on Chinese herbal medicine is original》), Huang sprout (《Fujian east book on Chinese herbal medicine》), yellow Cape jasmine Son (《Jiangsu medicinal material will》), yellow Cape jasmine, gardenia jasminoides ellis, mountain Cape jasmine (《Zhejiang medicinal plant will》)；For Rubiaceae cape jasmine platymiscium cape jasmine Fruit.

Wilsonii；Another name：Bank bent stick, tiger are hasty and careless, 100 pins.Shrub, high 1-6 rice；Branch is many.Leaf has a leaflet 5, and dilute 3； Petiole Chang Shusheng spinelet, vanelets papery, ellipticity obovate or Long Circle, tip are tapering, base portion wealthy wedge shape, above coarse, Bottle green, has coarse wool on arteries and veins, below light green, have pubescence on arteries and veins, there is sharp keen heavy sawtooth at edge；Petiolule has brown short soft Hair.The single basidixed of umbel, spends majority；Nothing hair, bennet slightly has hair nothing hair or base portion to total bennet；Spend purple yellow；Calyx is nothing hair； Petal is avette；Ovary Room 5, the whole symphysis of style become column.The spherical or ovoid of fruit.The month at florescence 6-7, the fruiting period 8-10 month.

Polygala root：Also known as around, etc..Produce northeast, North China, northwest and Central China and Sichuan；Perennial herb, main root are thick Strong, bast meat.With tranquilize the mind and promote the intelligence, eliminating the phlegm, detumescence function, the insomnia and dreamful sleep caused for breakdown of the normal physiological coordination between the heart and the kidney, forgetful frightened Throb with fear, wandering, expectoration is not well, sore swollen toxin, mastosis.

The root of herbaceous peony：Chinese herbaceous peony is spent in vain also referred to as, be Ranunculaceae Chinese herbaceous peony platymiscium.In the existing long cultivation history of China, in being renown far and wide Outward, which claims to spend Chinese herbaceous peony in vain.Its root can be used as medicine.Smooth liver and relieve pain, nourishing blood for regulating menstruation, astringing YIN to stop sweating.For dizziness of having a headache, hypochondriac pain, suffer from abdominal pain, Four limbs contraction pain, blood deficiency chlorosis, irregular menstruation, spontaneous perspiration, night sweat.

Chinese Fevervine Herb：Chinese Fevervine Herb refers generally to fevervine, is the herb of madder wort fevervine, and summer harvests herb, dries. Its sweet, slight bitter, mild-natured.The effect of with expelling wind and removing dampness, analgesic antidotal, promoting digestion and removing indigestion, activating blood circulation and reducing swelling.For rheumatism muscles and bones Bitterly, traumatic injury, traumatic pain, liver and gall and stomach intestinal colic, indigestion, infantile malnutrition, bronchitis；It is used for dermatitis outward, Eczema and sore swollen toxin.

In the medicine of the present invention there is no incompatibility in each material combination, can substantially reduce the generation of Rana grylia red leg disease Rate, has good preventive and therapeutic action to Rana grylia disease, and has no toxic side effect, using safety, residual nothing hazardous drugs Stay.

It is a further object of the present invention to provide a kind of preparation method of said medicine, its process is simple, its bulk drug is common, Cheaply.

The preparation method of medicine of the present invention is as follows：

The first step:The raw material weight of batch production is calculated by formula rate；

Second step:The raw material for calculating batch production is positioned in stirring container, be soaked in water 3-5 hour, warm fire heating is burnt To seething with excitement, 15-20 minute is endured, after being then incubated 3 hours, then warm fire heating is burnt to seething with excitement, and endures 25-30 minute, wherein the use of water Measure 30 times for Chinese medicine gross weight；

3rd step:Carry out centrifugal filtration, filtered fluid through 2-3 hour precipitation after, second centrifugal filtration；Filtered fluid is this The medicine of bright preventing and treating Rana grylia disease.

The medicine using method of the present invention：Become the frog phase, when feeding every time, every 10 kg diet, weigh 1000 grams of U.S. green grass or young crops The protective agents filtered fluid of frog red leg disease, adds 50 grams of brown sugar in Chinese medicine composition filtered fluid, is subsequently adding in feed, mixes All, feeding immediately, respectively feeding once sooner or later daily, free choice feeding, 10 days is a course for the treatment of, this medicine can be fed every 10 days One course for the treatment of, froglet phase medicine halve.

In order to the present invention is illustrated more clearly that, following examples is enumerated, but its scope to invention is without any restrictions.

Specific embodiment

Embodiment 1

Weigh rheum officinale 200g, shell of seaear 60g, toothed burclover 200g, Radix Astragali 200g, myrrh 200g, wormwood 100g, salvia chinensis 150g, cape jasmine 200g, wilsonii 120g, polygala root 130g, root of herbaceous peony 150g, Chinese Fevervine Herb 140g, these material compositions are the matter after drying Amount.

The first step:The raw material weight of batch production is calculated by formula rate；

Second step:The raw material for calculating batch production is positioned in stirring container, is soaked in water 4 hours, warm fire heating is burnt extremely Boiling, endures 20 minutes, and after being then incubated 3 hours, then warm fire heating is burnt to seething with excitement, and endures 30 minutes, and the wherein consumption of water is Chinese medicine 30 times of gross weight；

3rd step:Carry out centrifugal filtration, filtered fluid through 2 hours precipitation after, second centrifugal filtration；Filtered fluid is the present invention The medicine of preventing and treating Rana grylia disease.

Embodiment 2-12, comparative example 1-2

Embodiment 2-12 in addition to raw material mass mixture ratio is different from embodiment 1, its raw material type and step of preparation process and use Method is identical with embodiment 1, and comparative example 1 is to eliminate myrrh on the basis of embodiment 1, comparative example 2 be in embodiment 1 On the basis of eliminate salvia chinensis, raw material that embodiment 1-12, comparative example 1-2 are used and quality as shown in Table 1 below, below Table 1 shows the raw material and quality used by embodiment 1-12, comparative example 1-2.

Table 1

Clinical testing one：

(1) animal used as test

300 Rana grylia with red leg disease are selected to become the frog, substantially red point or erythema occurs in skin under abdomen, anorexia, no Hope activity.

(2) experimental technique

Random packet, is divided into 15 groups, and per organizing 20, wherein 14 groups is experimental group, respectively feeding embodiment 1-12, comparative example The feed that medicine described in 1-2 is made into, daily 2 times, free choice feeding, continuous 10 days；Another group is control group, using prior art Streptomysin cure, feeds according still further to normal feed mode.Treatment is observed and is made a record for 10 days afterwards.2 are specifically shown in Table.

(3) treatment standard and observational technique

Cure：Red leg disease is fully recovered；

Effectively：Red leg disease is effectively alleviated, and has very big alleviation.

Invalid：Red leg disease is not effectively alleviated.

Table 2

	Cure number	Significant figure	Invalid number
				Embodiment 1	19	0	1
Embodiment 2	20	0	0
				Embodiment 3	18	1	1
Embodiment 4	19	1	0
				Embodiment 5	19	1	0
Embodiment 6	20	0	0
				Embodiment 7	19	1	0
Embodiment 8	18	1	1
				Embodiment 9	19	1	0
Embodiment 10	19	1	0
				Embodiment 11	18	1	1
Embodiment 12	19	1	0
				Comparative example 1	14	4	2
Comparative example 2	13	5	2
				Control group	9	3	8

It follows that in the case of equal consumption, it is right that the therapeutic effect that is mixed into using medicine of the present invention in feed is substantially better than Ratio and control group.And after 1 month, experimental group is 0 recurrence, and contrast groups have 3 recurrences.

Clinical testing two：

In order to feeding effect of the present invention to Rana grylia is proved, feeding experiment is repeatedly tried, choose large-scale Rana grylia and support The Rana grylia for being in into the frog phase of field 3000 is grown, body weight size, health status are basically identical, and 15 groups are randomly divided into, per group 200 Only.Wherein 14 groups is experimental group, the feed that feeding embodiment 1-12, medicine described in comparative example 1-2 are made into respectively, twice daily, Free choice feeding, continuous 10 days；One course for the treatment of of this medicine was fed every 10 days.Another group is control group, and feeding is not added with the identical of medicine Feed, the nursing number of times of 15 groups of frogs, feeding volume, nursing time all same.The various performance indications of 15 groups of frogs are contrasted, averagely Value such as table 3.

After 1 month, 15 groups of frog situation such as tables 3：

Table 3

	Ill evil number	Moon weightening (g)/only
			Embodiment 1	0	120
Embodiment 2	7	109
			Embodiment 3	5	112
Embodiment 4	0	110
			Embodiment 5	0	119
Embodiment 6	0	120
			Embodiment 7	2	114
Embodiment 8	0	116
			Embodiment 9	4	113
Embodiment 10	0	115
			Embodiment 11	1	117
Embodiment 12	0	118
			Comparative example 1	20	97
Comparative example 2	30	90
			Control group	50	79

Can be evident that from table, after the medicine that the continuous feeding present invention is provided, daily gain is more right for experimental group frog Increase substantially according to group, and experimental group, compared with control group honey stomach, energetic, state is good, occurs without disease substantially.

So the present invention is not limited to the protective agents as Rana grylia red leg disease, as long as composition of raw materials and the present invention Identical or equivalent medicine just falls within the scope and spirit of the invention.By the preventing and treating with the Rana grylia red leg disease of the present invention The raw material of medicine same recipe is made any Chinese medicine preparation and is also fallen within the scope and spirit of the invention, as long as that is, raw material is joined Side is just fallen within the scope and spirit of the invention with identical medicine of the present invention.

Claims (7)

1. a kind of protective agents of Rana grylia red leg disease, it is characterised in that be made up of the bulk drug of following masses part proportioning：Greatly Yellow 170-210 part, shell of seaear 50-80 part, toothed burclover 190-220 part, Radix Astragali 180-215 part, myrrh 180-210 part, wormwood 80- 110 parts, salvia chinensis 130-165 part, cape jasmine 185-215 part, wilsonii 105-135 part, polygala root 105-140 part, root of herbaceous peony 135-160 Part, Chinese Fevervine Herb 120-150 part.

2. protective agents of Rana grylia red leg disease according to claim 1, it is characterised in that rheum officinale 170-205 part, stone Cassia 50-75 part, toothed burclover 190-215 part, Radix Astragali 190-215 part, myrrh 185-210 part, wormwood 80-105 part, salvia chinensis 130- 160 parts, cape jasmine 185-210 part, wilsonii 105-130 part, polygala root 110-140 part, root of herbaceous peony 140-160 part, Chinese Fevervine Herb 125-150 Part.

3. protective agents of Rana grylia red leg disease according to claim 1, it is characterised in that rheum officinale 180-205 part, stone Cassia 50-70 part, toothed burclover 190-210 part, Radix Astragali 190-210 part, myrrh 190-210 part, wormwood 90-105 part, salvia chinensis 140- 160 parts, cape jasmine 190-210 part, wilsonii 110-130 part, polygala root 120-140 part, root of herbaceous peony 145-160 part, Chinese Fevervine Herb 130-150 Part.

4. protective agents of Rana grylia red leg disease according to claim 1, it is characterised in that rheum officinale 190-205 part, stone Cassia 55-70 part, toothed burclover 190-205 part, Radix Astragali 195-210 part, myrrh 190-205 part, wormwood 95-105 part, salvia chinensis 140- 155 parts, cape jasmine 190-205 part, wilsonii 110-125 part, polygala root 120-135 part, root of herbaceous peony 145-155 part, Chinese Fevervine Herb 135-150 Part.

5. protective agents of Rana grylia red leg disease according to claim 1, it is characterised in that rheum officinale 190-200 part, stone Cassia 60-70 part, toothed burclover 190-200 part, Radix Astragali 200-210 part, myrrh 190-200 part, wormwood 100-105 part, salvia chinensis 150- 155 parts, cape jasmine 200-205 part, wilsonii 110-120 part, polygala root 130-135 part, root of herbaceous peony 145-150 part, Chinese Fevervine Herb 140-150 Part.

6. protective agents of Rana grylia red leg disease according to claim 1, it is characterised in that 200 parts of rheum officinale, the shell of seaear 60 parts, 200 parts of toothed burclover, 200 parts of the Radix Astragali, 200 parts of myrrh, 100 parts of wormwood, 150 parts of salvia chinensis, 200 parts of cape jasmine, 120 parts of wilsonii, 130 parts of polygala root, 150 parts of the root of herbaceous peony, 140 parts of Chinese Fevervine Herb.

7. the preparation method of protective agents of Rana grylia red leg disease according to claim 1, it is characterised in that including as follows Step:

The first step:The raw material weight of batch production is calculated by formula rate；

Second step:The raw material for calculating batch production is positioned in stirring container, be soaked in water 3-5 hour, warm fire heating is burnt To seething with excitement, 15-20 minute is endured, after being then incubated 3 hours, then warm fire heating is burnt to seething with excitement, and endures 25-30 minute, wherein the use of water Measure 30 times for Chinese medicine gross weight；

3rd step:Carry out centrifugal filtration, filtered fluid through 2-3 hour precipitation after, second centrifugal filtration；Filtered fluid is the U.S. The protective agents of frog disease.