CN106442531B - A method of distinguishing Bowring cattleya flower quality - Google Patents
A method of distinguishing Bowring cattleya flower quality Download PDFInfo
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- CN106442531B CN106442531B CN201610964025.2A CN201610964025A CN106442531B CN 106442531 B CN106442531 B CN 106442531B CN 201610964025 A CN201610964025 A CN 201610964025A CN 106442531 B CN106442531 B CN 106442531B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8466—Investigation of vegetal material, e.g. leaves, plants, fruits
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Abstract
The invention belongs to plant quality identification technology fields, specifically disclose a kind of method for distinguishing Bowring cattleya flower quality, include the following steps: that (1) chooses Bowring cattleya petal to be measured, prepare the histotomy in petal cross section；(2) the epidermal cell shape and arrayed feature of Bowring cattleya flower to be measured are observed, if epidermal cell is in smooth shape, and gapless between cell, the material of the petal of Bowring cattleya to be measured are smooth material；If epidermal cell is in there is gap between circular cone or dome-shaped and cell, the material of the petal of Bowring cattleya to be measured is soft papery；If epidermal cell, which is presented, gap between spininess or triangle and cell, the material of the petal of Bowring cattleya to be measured is velvet or microvillus texture.Present invention firstly discovers that the position of differing texture difference has the epidermal cell of different shape and arrayed feature on Bowring cattleya flower, therefore, a kind of method for distinguishing Bowring cattleya flower quality is founded, this method can provide a certain basis for the fancy points research of Bowring cattleya and resistance mechanism study.
Description
Technical field
The present invention relates to plant quality identification technology fields, and in particular, to a kind of side for distinguishing Bowring cattleya flower quality
Method.
Background technique
Bowring cattleya (Cattleya) belong to orchid family epiphytic orchid class, american torrid zone and subtropical zone are originated in, because of the gorgeous spot of its pattern
Bright-coloured spends very large mostly fragrance, and especially cross compatibility is extremely strong, maximum, the most gorgeous kind of flower in existing hot bandwidth, and spends more
Middle or small flower pattern kind；The kind of existing soft such as paper, and have the smooth kind such as wax of flower；And have throughout the year not
It persistently blooms with kind, and good reputation is " king of hot bandwidth ", Bowring cattleya platymiscium has good breeding advantage and has out very much
Send out the commercial value of potentiality.
Flower not only plays key to the existence procreation of Bowring cattleya as the most important reproduction of Bowring cattleya and ornamental organ
Effect, also can be improved the ornamental value and economic value of Bowring cattleya.The kind of the existing flower softness such as paper of Bowring cattleya at present, and have
The smooth kind such as wax of flower, veteran technical specialist can rely on the experience of many years, can judge from sense organ
The quality of Bowring cattleya flower, still, those skilled in the art are difficult to accomplish this point, therefore, lack in the prior art a kind of general
The method that adaptive preferably distinguishes Bowring cattleya flower quality.
Summary of the invention
The purpose of the invention is to overcome the deficiencies of the prior art and provide a kind of side for distinguishing Bowring cattleya flower quality
Method.
To achieve the goals above, the present invention is achieved by the following technical programs:
A method of Bowring cattleya flower quality is distinguished, is included the following steps:
(1) petal for choosing Bowring cattleya to be measured, prepares the histotomy in petal cross section；
(2) the epidermal cell shape and arrayed feature of Bowring cattleya flower to be measured are observed, if epidermal cell is in smooth shape, and
Gapless between cell, the material of the petal of Bowring cattleya to be measured are smooth material；If epidermal cell in circular cone or dome-shaped and
There is gap between cell, the material of the petal of Bowring cattleya to be measured is soft papery；If spininess or triangle is presented in epidermal cell
There is gap between shape and cell, the material of the petal of Bowring cattleya to be measured is velvet or microvillus texture.
In order to more easily carry out histotomy fiber observation, it is preferable that choose Bowring cattleya full-bloom stage to be measured in step (1)
Petal, prepare the histotomy in petal cross section.
The histotomy in petal cross section is prepared for more criterion, it is preferable that with reference to (2003) such as Rasika G.
Method prepares the histotomy in petal cross section.
The histotomy in petal cross section is prepared for more criterion, it is highly preferred that preparing petal using following method
The histotomy in cross section: it cuts 0.1~0.2 millimeter of petal and does cross section, be immersed in 0.25% with the petal that will be cut
In the solution of PEG-4000.
Compared with prior art, the invention has the following beneficial effects:
Present invention firstly discovers that the position of differing texture difference has different shape and arrayed feature on Bowring cattleya flower
Therefore epidermal cell has founded a kind of method for distinguishing Bowring cattleya flower quality, this method can be the fancy points of Bowring cattleya
Research and resistance mechanism study provide a certain basis.
Detailed description of the invention
Fig. 1 is Bowring cattleya 2 and represents germplasm blossom character Characteristic Contrast.
Fig. 2 is Bowring cattleya 2 and represents germplasm flower unlike material position epidermal cell Characteristic Contrast.
Specific embodiment
The present invention is made with specific embodiment with reference to the accompanying drawings of the specification and further being elaborated, the embodiment
It is served only for explaining the present invention, be not intended to limit the scope of the present invention.Test method as used in the following examples is such as without spy
Different explanation, is conventional method；Used material, reagent etc., unless otherwise specified, for the reagent commercially obtained
And material.
Embodiment 1
1. in the famous-brand and high-quality Ornamental Plants Germplasm Resources garden capture card in Guangdong Province special No. 1 and the special No. 2 full-bloom stage flowers of card.Two cards
The mode of appearance feature of special orchid sample is described as follows: card special No. 1 is Bowring cattleya kind caused by selecting TaiwanPotinara. Haw
Maternal (♀) is done in the Yongkang Yuan Gold ' Y.K. #2 ' 2,Laeliocattleya.Trick or Treat does male parent (♂)
Hybridization F1In generation, medium-sized flower, pure orange, no piebald, petal epidermis is smooth glossy, and the florescence sustainable 2~3 months；Card is No. 2 special
It is the commercial variety 689 bought from market#It does maternal (♀), 167#It is the hybridization F of male parent (♂)1Generation, great Hua, aubergine, petal
Papery is soft, and lip prune simultaneously has, and epidermis has thick velvet material like frivolous velvet material, especially lip
Sense, spending has fragrant giving off a strong fragrance, and the florescence sustainable 2 months.
2. the observation method of petal anatomical structure: preparing petal cross section with reference to the method for (2003) such as Rasika G.
Histotomy, laboratory sample choose full-bloom stage petal, petal (about 0.1~0.2 millimeter) is cut using sharp blade, with
The petal that will be cut is immersed in 0.25% polyglycol solution (molecular weight 4000), using Zeiss Axio Scope A1
Microscope is just being set to be observed and photographed to record.
3. result and analysis:
The mode of appearance comparison of card spy No. 1 and special No. 2 two samples of card is as shown in Figure 1.Observe by the naked eye two Ka Te
The mode of appearance of orchid species matter flower, it is possible to find under same visual environment, special No. 1 petal of card has a degree of smooth reflective
Sense, and block special No. 2 petal no-reflection phenomenons；In sense of touch, special No. 1 petal of card is more smooth stiffening, and blocks special No. 2 petals
Frivolous soft such as paper, wherein lip has thick velvet sense of touch.The specific descriptions of two sample morphology characters and measurement are shown in Table 1 institute
Show.
Table 1 is the relevant information of 2 Bowring cattleya germplasm
Two Bowring cattleya flower epidermis characteristic comparisons under optical microscopy: find that two Bowring cattleyas are miscellaneous by micro- sem observation
Handing over the flower epidermal cell of kind has different structure features, and result of study is as shown in Figure 2.The card spy 1 of the smooth material of petal
Number, epidermal cell is in smooth shape, and is in intercellular gap very little；The petal sections of soft papery, epidermal cell is in cone
And space between cells is larger；And blocking the lip part of special No. 2 tools swan velour texture, spininess and space between cells is presented in epidermal cell
It is very big.Result of study shows that flower epidermal cell shape will affect flower from space between cells size and form different appearance materials.
The present invention by the position of differing texture difference on above-mentioned research discovery Bowring cattleya flower have different shape and
The epidermal cell of arrayed feature, therefore, the epidermal cell shape and arrayed feature of flower, which can be used as, distinguishes Bowring cattleya flower matter
The standard on ground.
Embodiment 2
A method of Bowring cattleya flower quality is distinguished, is included the following steps:
(1) petal for choosing Bowring cattleya full-bloom stage to be measured prepares petal cross with reference to the method for Rasika G. etc. (2003)
The histotomy of section, specific method are to cut petal using sharp blade and do cross section (about 0.1~0.2 millimeter), immediately
The petal cut is immersed in 0.25% PEG(polyethylene glycol) in solution (molecular weight 4000), using Zeiss Axio Scope
A1 is just setting microscope and is being observed and photographed to record.
(2) the epidermal cell shape and arrayed feature of optical microphotograph microscopic observation Bowring cattleya flower to be measured, if epidermis is thin
Born of the same parents are in smooth shape, and gapless between cell, the material of the petal of Bowring cattleya to be measured are smooth material；If epidermal cell is in circle
There is gap between cone or dome-shaped and cell, the material of the petal of Bowring cattleya to be measured is soft papery；If epidermal cell is presented
There is gap between spininess or triangle and cell, the material of the petal of Bowring cattleya to be measured is velvet or microvillus texture.
Claims (1)
1. a kind of method for distinguishing Bowring cattleya flower quality, which comprises the steps of:
(1) petal for choosing Bowring cattleya full-bloom stage to be measured, cuts 0.1~0.2 millimeter of petal and does cross section, with the flower that will be cut
Valve is immersed in the solution of 0.25% PEG-4000, and the molecular weight of PEG-4000 is 4000, and the tissue for preparing petal cross section is cut
Piece is just being set micro- sem observation using Zeiss Axio Scope A1 and is being photographed to record；
(2) the epidermal cell shape and arrayed feature of Bowring cattleya flower to be measured are observed, if epidermal cell is in smooth shape, and cell
Between gapless, the material of the petal of Bowring cattleya to be measured is smooth material；If epidermal cell is in circular cone or dome-shaped and cell
Between have a gap, the material of the petal of Bowring cattleya to be measured is soft papery；If epidermal cell present spininess or triangle and
There is gap between cell, the material of the petal of Bowring cattleya to be measured is velvet or microvillus texture.
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| CN106442531B true CN106442531B (en) | 2019-02-26 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103487439A (en) * | 2013-09-23 | 2014-01-01 | 北京农业信息技术研究中心 | Plant leaf apparent grain and texture information acquisition device and acquisition method thereof |
| CN103512784A (en) * | 2013-09-18 | 2014-01-15 | 北京林业大学 | Preparation method of plant tissue section, plant tissue section and application thereof |
| CN105699142A (en) * | 2016-02-18 | 2016-06-22 | 石河子大学 | Paraffin section making method for close-texture hard plant materials |
-
2016
- 2016-10-28 CN CN201610964025.2A patent/CN106442531B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103512784A (en) * | 2013-09-18 | 2014-01-15 | 北京林业大学 | Preparation method of plant tissue section, plant tissue section and application thereof |
| CN103487439A (en) * | 2013-09-23 | 2014-01-01 | 北京农业信息技术研究中心 | Plant leaf apparent grain and texture information acquisition device and acquisition method thereof |
| CN105699142A (en) * | 2016-02-18 | 2016-06-22 | 石河子大学 | Paraffin section making method for close-texture hard plant materials |
Non-Patent Citations (3)
| Title |
|---|
| Pigment Distribution and Epidermal Cell Shape in Dendrobium Species and Hybrids;Rasike G. Mudalige等;《HORTSCIENCE》;20031231;第573页"Preparation of tissue section and analysis"部分 * |
| Relationship between the velvet-like texture of flower petals and light reflection from epidermal cell surfaces;Yang Zhang等;《J Plant Res》;20150426;第624-631页 * |
| 蝴蝶兰花瓣的扫描电镜观察;李佐 等;《中国观赏园艺研究进展》;20141231;第1-2节 * |
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