CN106442531A - Cattleya hybrida flower texture distinguishing method - Google Patents
Cattleya hybrida flower texture distinguishing method Download PDFInfo
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- CN106442531A CN106442531A CN201610964025.2A CN201610964025A CN106442531A CN 106442531 A CN106442531 A CN 106442531A CN 201610964025 A CN201610964025 A CN 201610964025A CN 106442531 A CN106442531 A CN 106442531A
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- petal
- cattleya
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- bowring cattleya
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/84—Systems specially adapted for particular applications
- G01N2021/8466—Investigation of vegetal material, e.g. leaves, plants, fruits
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Abstract
The invention belongs to the technical field of plant quality identification, and particularly discloses a Cattleya hybrida flower texture distinguishing method. The method comprises the following steps: (1) selecting a Cattleya hybrida petal to be detected, and preparing a petal cross section tissue section; and (2) observing the shape and arrangement characteristics of epidermal cells of the Cattleya hybrida flower to be detected, if the epidermal cells are flat and no gap is formed therebetween, judging that the Cattleya hybrida petal to be detected has a smooth texture; if the epidermal cells are conical or circular arch shaped and gaps are formed therebetween, judging that the Cattleya hybrida petal to be detected has a soft paper texture; and if the epidermal cells are spiny or triangular and gaps are formed therebetween, judging that the Cattleya hybrida petal to be detected has a velvet or microvillus texture. The invention makes the first discovery that the part of the Cattleya hybrida flower with different textures has the epidermal cells having different shape and arrangement characteristics, thereby creating the Cattleya hybrida flower texture distinguishing method; and the method can provide a certain basis for fancy point research and stress tolerance mechanism research of Cattleya hybrida.
Description
Technical field
A kind of the present invention relates to plant quality identification technology field, in particular it relates to side distinguishing Bowring cattleya flower quality
Method.
Background technology
Bowring cattleya（Cattleya）Belong to orchid family epiphytic orchid class, originate in american torrid zone and subtropical zone, because of the gorgeous spot of its pattern
Bright-coloured, spends very large how fragrant, especially cross compatibility is extremely strong, and in existing hot bandwidth, maximum, the most gorgeous kind of flower, spends more again
Middle or small flower pattern kind；The existing soft kind as paper, has the smooth kind as wax of flower again；And have throughout the year not
Persistently bloom with kind, and good reputation is " king of hot bandwidth ", Bowring cattleya platymiscium has good breeding advantage and has out very much
Send out the commercial value of potentiality.
Flower as the most important reproduction of Bowring cattleya and views and admires organ, and the not only existence procreation to Bowring cattleya plays key
Effect, also can improve ornamental value and the economic worth of Bowring cattleya.The soft kind as paper of the existing flower of Bowring cattleya, has again at present
The smooth kind as wax of flower, veteran technical specialist just can be able to judge from sense organ by experience for many years
The quality of Bowring cattleya flower, but, those skilled in the art are difficult to accomplish this point, therefore, lack a kind of general in prior art
Adaptive preferably distinguish Bowring cattleya flower quality method.
Content of the invention
The invention aims to overcoming the deficiencies in the prior art, provide a kind of distinguish Bowring cattleya flower quality side
Method.
To achieve these goals, the present invention is achieved by the following technical programs：
A kind of method distinguishing Bowring cattleya flower quality, comprises the steps：
(1) choose the petal of Bowring cattleya to be measured, prepare the cross section histotomy of petal；
(2) epidermal cell shape and the arrayed feature of Bowring cattleya flower to be measured are observed, if epidermal cell is in smooth shape, and cell
Between gapless, the material of the petal of Bowring cattleya to be measured is smooth material；If epidermal cell is in circular cone or dome-shaped and cell
Between have a gap, the material of the petal of Bowring cattleya to be measured is soft papery；If epidermal cell present spininess or triangle and
There is gap, the material of the petal of Bowring cattleya to be measured is velvet or microvillus texture between cell.
In order to more easily carry out histotomy fiber observation it is preferable that choosing Bowring cattleya full-bloom stage to be measured in step (1)
Petal, prepare the cross section histotomy of petal.
In order to more criterion prepare the cross section histotomy of petal it is preferable that with reference to Rasika G. etc.（2003）'s
Method is preparing the cross section histotomy of petal.
In order to more criterion prepare the cross section histotomy of petal, it is highly preferred that preparing petal using following method
Cross section histotomy：Cut 0.1～0.2 millimeter of petal and do cross section, be immersed in 0.25% with the petal that will cut
In the solution of PEG-4000.
Compared with prior art, the present invention has the advantages that：
Present invention firstly discovers that the position of differing texture difference has the epidermis of different shape and arrayed feature on Bowring cattleya flower
Cell, therefore, has founded a kind of method distinguishing Bowring cattleya flower quality, and the method can be the fancy points research of Bowring cattleya
And resistance study mechanism provides certain foundation.
Brief description
Fig. 1 represents germplasm blossom character Characteristic Contrast for Bowring cattleya 2.
Fig. 2 represents germplasm flower unlike material position epidermal cell Characteristic Contrast for Bowring cattleya 2.
Specific embodiment
With reference to Figure of description and specific embodiment, the present invention is made and further elaborating, described embodiment
It is served only for explaining the present invention, be not intended to limit the scope of the present invention.Test method used in following embodiments is such as no special
Different explanation, is conventional method；The material that used, reagent etc., if no special instructions, for the reagent commercially obtaining
And material.
Embodiment 1
1. in the full-bloom stage flower of Guangdong Province famous-brand and high-quality Ornamental Plants Germplasm Resources garden capture card spy No. 1 and special No. 2 of card.Two Bowring cattleyas
The mode of appearance feature interpretation of sample is as follows：Special No. 1 of card is the Bowring cattleya kind being caused from TaiwanPotinara.Haw
Female parent is done in Yuan Gold ' Y.K. #2 ' Yongkang 2（♀）,Laeliocattleya.Trick or Treat does male parent（♂）
Hybridization F1Generation, medium-sized flower, pure orange, no piebald, petal epidermis is smooth glossy, sustainable 2～3 months of florescence；Special No. 2 of card
It is the commercial variety 689 bought from market#Do female parent（♀）, 167#Do male parent（♂）Hybridization F1Generation, great Hua, aubergine, petal
Papery is soft, and lip prune simultaneously carries, and epidermis has thick velvet material just as frivolous velvet material, especially lip
Sense, flower has fragrant giving off a strong fragrance, sustainable 2 months of florescence.
2. the observational technique of petal anatomical structure：With reference to Rasika G. etc.（2003）Method preparing petal cross section
Histotomy, laboratory sample choose full-bloom stage petal, petal is cut using sharp blade（About 0.1～0.2 millimeter）, with
The petal that will cut is immersed in 0.25% polyglycol solution（Molecular weight 4000）, using Zeiss Axio Scope A1
Just putting microscope to carry out observing and Taking Pictures recording.
3. result and analysis：
Special No. 1 mode of appearance with special No. 2 two samples of card of card contrasts as shown in Figure 1.Observe by the naked eye two Bowring cattleya kinds
The mode of appearance of matter flower, it is possible to find under same visual environment, the petal that card is special No. 1 has a certain degree of smooth reflective sense,
And block the petal no-reflection phenomenon of special No. 2；In sense of touch, the petal that card is special No. 1 is more smooth stiffening, and the petal blocking special No. 2 is light
Thin soft such as paper, wherein lip has thick velvet sense of touch.The specific descriptions of two sample morphology proterties and mensure are shown in Table 1.
Table 1 is the relevant information of 2 Bowring cattleya germplasm
Two Bowring cattleya flower epidermis characteristic contrasts under light microscope：Find that two Bowring cattleyas hybridize product by micro- sem observation
The flower epidermal cell planted has different architectural features, and result of study is as shown in Figure 2.Special No. 1 of the card of the smooth material of petal, table
Chrotoplast is in smooth shape, and is in intercellular gap very little；The petal sections of soft papery, epidermal cell is in cone and cell
Gap is larger；And blocking the special No. 2 lip parts having swan velour textures, its epidermal cell assumes spininess and space between cells is very big.
Result of study shows that flower epidermal cell shape can affect flower from space between cells size and form different outward appearance materials.
The present invention by the position that above-mentioned research finds differing texture difference on Bowring cattleya flower have different shape and
The epidermal cell of arrayed feature, therefore, the epidermal cell shape of flower and arrayed feature can be used as differentiation Bowring cattleya flower matter
The standard on ground.
Embodiment 2
A kind of method distinguishing Bowring cattleya flower quality, comprises the steps：
(1) choose the petal of Bowring cattleya full-bloom stage to be measured, with reference to Rasika G. etc.（2003）Method preparing petal cross section
Histotomy, concrete grammar is to cut petal using sharp blade and do cross section（About 0.1～0.2 millimeter）, with will cut
The petal taking is immersed in 0.25% PEG（Polyethylene glycol）In solution（Molecular weight 4000）, using Zeiss Axio Scope A1
Just putting microscope to carry out observing and Taking Pictures recording.
(2) the epidermal cell shape of optical microphotograph Microscopic observation Bowring cattleya to be measured flower and arrayed feature, if epidermis is thin
Born of the same parents are in smooth shape, and gapless between cell, and the material of the petal of Bowring cattleya to be measured is smooth material；If epidermal cell is in circle
There is gap, the material of the petal of Bowring cattleya to be measured is soft papery between cone or dome-shaped and cell；If epidermal cell presents
There is gap, the material of the petal of Bowring cattleya to be measured is velvet or microvillus texture between spininess or triangle and cell.
Claims (3)
1. a kind of method distinguishing Bowring cattleya flower quality is it is characterised in that comprise the steps：
(1) choose the petal of Bowring cattleya to be measured, prepare the cross section histotomy of petal；
(2) epidermal cell shape and the arrayed feature of Bowring cattleya flower to be measured are observed, if epidermal cell is in smooth shape, and cell
Between gapless, the material of the petal of Bowring cattleya to be measured is smooth material；If epidermal cell is in circular cone or dome-shaped and cell
Between have a gap, the material of the petal of Bowring cattleya to be measured is soft papery；If epidermal cell present spininess or triangle and
There is gap, the material of the petal of Bowring cattleya to be measured is velvet or microvillus texture between cell.
2. method according to claim 1 it is characterised in that step (1) in choose Bowring cattleya full-bloom stage to be measured petal,
Prepare the cross section histotomy of petal.
3. method according to claim 1 and 2 is it is characterised in that prepare the cross section tissue of petal using following method
Section：Cut 0.1～0.2 millimeter of petal and do cross section, be immersed in the solution of 0.25% PEG-4000 with the petal that will cut
In.
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| CN201610964025.2A CN106442531B (en) | 2016-10-28 | 2016-10-28 | A method of distinguishing Bowring cattleya flower quality |
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| CN201610964025.2A CN106442531B (en) | 2016-10-28 | 2016-10-28 | A method of distinguishing Bowring cattleya flower quality |
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| CN106442531B CN106442531B (en) | 2019-02-26 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103487439A (en) * | 2013-09-23 | 2014-01-01 | 北京农业信息技术研究中心 | Plant leaf apparent grain and texture information acquisition device and acquisition method thereof |
| CN103512784A (en) * | 2013-09-18 | 2014-01-15 | 北京林业大学 | Preparation method of plant tissue section, plant tissue section and application thereof |
| CN105699142A (en) * | 2016-02-18 | 2016-06-22 | 石河子大学 | Paraffin section making method for close-texture hard plant materials |
-
2016
- 2016-10-28 CN CN201610964025.2A patent/CN106442531B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103512784A (en) * | 2013-09-18 | 2014-01-15 | 北京林业大学 | Preparation method of plant tissue section, plant tissue section and application thereof |
| CN103487439A (en) * | 2013-09-23 | 2014-01-01 | 北京农业信息技术研究中心 | Plant leaf apparent grain and texture information acquisition device and acquisition method thereof |
| CN105699142A (en) * | 2016-02-18 | 2016-06-22 | 石河子大学 | Paraffin section making method for close-texture hard plant materials |
Non-Patent Citations (3)
| Title |
|---|
| RASIKE G. MUDALIGE等: "Pigment Distribution and Epidermal Cell Shape in Dendrobium Species and Hybrids", 《HORTSCIENCE》 * |
| YANG ZHANG等: "Relationship between the velvet-like texture of flower petals and light reflection from epidermal cell surfaces", 《J PLANT RES》 * |
| 李佐 等: "蝴蝶兰花瓣的扫描电镜观察", 《中国观赏园艺研究进展》 * |
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