CN106434617A - Thiobacillus microcapsule inoculant used for water purification and preparing method thereof - Google Patents
Thiobacillus microcapsule inoculant used for water purification and preparing method thereof Download PDFInfo
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- CN106434617A CN106434617A CN201610764500.1A CN201610764500A CN106434617A CN 106434617 A CN106434617 A CN 106434617A CN 201610764500 A CN201610764500 A CN 201610764500A CN 106434617 A CN106434617 A CN 106434617A
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- sulfide
- microcapsule
- oxidizing enzyme
- fermentation liquid
- water purification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
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- Genetics & Genomics (AREA)
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- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
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- Dispersion Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Manufacturing Of Micro-Capsules (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention discloses a thioacillus microcapsule inoculant used for water purification. The inoculant comprises a microcapsule and a fermentation liquor of thioacillus. The microcapsule is prepared from, by weight, 0.15%-0.17% of gelatin, 0.09%-0.12% cyclodextrin, 0.03-0.05% sodium alginate and 0.05%-0.75% of polyethylene glycol 4000. According to the thioacillus microcapsule inoculant used for water purification, a high polymer material is adopted to form a microcapsule protective film on the surface of the thioacillus, the thioacillus can be separated from the external environment, storage time of the thioacillus can be reduced, and bacterial activity can be improved, meanwhile, the thioacillus microcapsule inoculant can be dispersed in water, can be quickly disintegrated and evenly release the thioacillus, and has high purification efficiency.
Description
Technical field
The invention belongs to ecological, environmental protective technical field and in particular to a kind of sulfide-oxidizing enzyme microcapsule microbial agent for water purification and
Its preparation method, for purifying to water body.
Background technology
Sulfide-oxidizing enzyme is the prokaryote Pseudomonas that low price sulfur can be oxidized to high price sulfur by the relatively conventional class of microbial world,
They can be divided into two big class：Colorless sulfur bacteria and coloured sulfur bacteria.Colorless sulfur bacteria includes Thiobacillus
(Thiobacillus) they are that in soil and water, most important chemosynthetic bacteria being capable of oxidation of sulfureted hydrogen, pyrite, element
Sulfur etc. forms sulphuric acid, obtains energy from oxidizing process.Common are thiobacillus thiooxidans (T.thiooxidans) and oxidation is sub-
Ferrum Thiobacillus (T.ferrooxidans), grate sulfur thiobacillus (T.thioparus) etc..Coloured sulfur bacteria refers mainly to containing photosynthetic color
The sulfur bacteria of the utilization luminous energy nutrition of element, they obtain energy from light, rely on and contain special photosynthetic pigments in vivo, carry out light
Cooperation assimilation CO2.Coloured Thiobacillus includes photosynthetic autotrophs bacterium, and (this kind of photosynthetic bacteria, can be with unit when carrying out photosynthesis
Plain sulfur and sulfide are as assimilation CO2Electron donor) and photoheterotrophy type.Sulfide-oxidizing enzyme has weight in industry and field of Environment Protection
The economy wanted and social meaning, at the process of high concentrated organic wastewater, flue gas desulfurization, the removal of Heavy Metals in Sludge etc. aspect
All it is used widely.
Traditional sulfide-oxidizing enzyme immobilization technology mainly adds excipient in concentrating bacterium solution, makes bacterium after mix homogeneously
Agent granule, this method presence fixation after cure bacterial activity is low, is difficult to disperse, the not good grade of clean-up effect lacks after putting in water
Fall into.
Content of the invention
The purpose of the present invention is to overcome the fixing after cure bacterial activity of existing sulfide-oxidizing enzyme immobilization technology low, puts in water
After be difficult to disperse, the not good problem of clean-up effect.
For this reason, the invention provides a kind of sulfide-oxidizing enzyme microcapsule microbial agent for water purification, thin including microcapsule and sulfuration
The fermentation liquid of bacterium, described microcapsule includes the following component based on fermentation liquid percentage by weight：1.5 ‰～1.7 ‰ gelatin,
0.9 ‰～1.2 ‰ cyclodextrin, 0.3 ‰～0.5 ‰ sodium alginate, 5 ‰～7.5 ‰ Macrogol 4000.
Further, above-mentioned microcapsule includes the following component based on fermentation liquid percentage by weight：1.5 ‰～1.6 ‰
Gelatin, 0.9 ‰～1.0 ‰ cyclodextrin, 0.3 ‰～0.4 ‰ sodium alginate, 7 ‰～7.5 ‰ Macrogol 4000.
Further, above-mentioned microcapsule includes the following component based on fermentation liquid percentage by weight：1.5 ‰ gelatin,
1.0 ‰ cyclodextrin, 0.5 ‰ sodium alginate, 7 ‰ Macrogol 4000.
In addition, present invention also offers a kind of preparation method of the sulfide-oxidizing enzyme microcapsule microbial agent for water purification, including such as
Lower step：
1) sulfide-oxidizing enzyme is added to be fermented in fermentation liquid.
2) step 1) filter out solidss in fermentation liquid after the completion of fermentation, cryoconcentration is carried out to fermentation liquid.
3) in fermentation liquid after concentration, gelatin, cyclodextrin, sodium alginate, Macrogol 4000 are added, stirring and dissolving is equal
Through emulsifying after even, it is spray-dried and makes sulfide-oxidizing enzyme microcapsule microbial agent.
Further, above-mentioned steps 1) in the mass ratio of sulfide-oxidizing enzyme and fermentation liquid be 0.3 ‰～0.4 ‰.
Further, above-mentioned steps 2) in cryoconcentration temperature be 40～50 DEG C.
Further, above-mentioned steps 2) in cryoconcentration adopt vacuum distillation, the concentration after concentration be 10～20%.
Further, above-mentioned steps 3) in emulsion process carry out in high speed shear dispersion emulsifying machine.
The microcapsule of the present invention is formed by the way of being spray-dried, and gelatin, as emulsifying agent and film former, promotes stable
The formation of emulsion, and participate in encapsulation, cyclodextrin, as emulsifying agent, participates in encapsulation, sodium alginate is as steady
Determine agent, Macrogol 4000 is as sulfide-oxidizing enzyme microcapsule wall material.Sodium alginate and gelatin synergism, through shearing dispersion breast
After change, Macrogol 4000 and cyclodextrin is made to be dispersed in around sulfide-oxidizing enzyme, gelatin may additionally facilitate film forming, spray-dried
Form sulfide-oxidizing enzyme microcapsule afterwards.
Compared with prior art, beneficial effects of the present invention：
(1) this sulfide-oxidizing enzyme microcapsule microbial agent for water purification that the present invention provides adopts macromolecular material thin in sulfuration
Phage surface forms a microcapsule protecting film, can keep apart sulfide-oxidizing enzyme body and external environment, extends sulfide-oxidizing enzyme
Storage time, and improve its bacterial activity.
(2) this sulfide-oxidizing enzyme microcapsule microbial agent for water purification that the present invention provides easily disperses in water, and meeting water can be fast
Fast disintegrate simultaneously uniformly discharges sulfide-oxidizing enzyme, has higher purification efficiency.
(3) preparation method of this sulfide-oxidizing enzyme microcapsule microbial agent for water purification that the present invention provides adopts microcapsule skill
Art, operating process is simple and environmentally-friendly.
Specific embodiment
Below the embodiment it is clear that described is clearly and completely described to the technical scheme in the embodiment of the present invention
It is only a part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, the common skill in this area
All other embodiment that art personnel are obtained under the premise of not making creative work, broadly falls into the model of present invention protection
Enclose.
Embodiment 1：
Present embodiments provide a kind of preparation method of the sulfide-oxidizing enzyme microcapsule microbial agent for water purification, walk including following
Suddenly：
1) 0.3g sulfide-oxidizing enzyme is added to be fermented in 1000g fermentation liquid.
2) step 1) fermentation after the completion of, filter out the solidss in fermentation liquid, and at a temperature of 40 DEG C using decompression steam
The mode evaporating carries out cryoconcentration to fermentation liquid, and the concentration after concentration is 10%.
3), in fermentation liquid after concentration, add the weight than the gelatin for 1.5 ‰ and fermentation liquid with the weight of fermentation liquid
Than the cyclodextrin for 0.9 ‰, with fermentation liquid weight than the sodium alginate for 0.3 ‰, with the weight of fermentation liquid than the poly- second for 5 ‰
Glycol 4000, stirring and dissolving is uniformly divided using high speed shear afterwards and is spread mulser and carry out emulsifying, is finally spray-dried that to make sulfuration thin
Strain microcapsule microbial agent.
Embodiment 2：
Present embodiments provide a kind of preparation method of the sulfide-oxidizing enzyme microcapsule microbial agent for water purification, walk including following
Suddenly：
1) 0.4g sulfide-oxidizing enzyme is added to be fermented in 1000g fermentation liquid.
2) step 1) fermentation after the completion of, filter out the solidss in fermentation liquid, and at a temperature of 55 DEG C using decompression steam
The mode evaporating carries out cryoconcentration to fermentation liquid, and the concentration after concentration is 20%.
3), in fermentation liquid after concentration, add the weight than the gelatin for 1.7 ‰ and fermentation liquid with the weight of fermentation liquid
Than the cyclodextrin for 1.2 ‰, with fermentation liquid weight than the sodium alginate for 0.5 ‰, with the weight of fermentation liquid than poly- for 7.5 ‰
Ethylene glycol 4000, stirring and dissolving is uniformly divided using high speed shear afterwards and is spread mulser and carry out emulsifying, is finally spray-dried and makes sulfuration
Antibacterial microcapsule microbial agent.
Embodiment 3：
Present embodiments provide a kind of preparation method of the sulfide-oxidizing enzyme microcapsule microbial agent for water purification, walk including following
Suddenly：
1) 0.35g sulfide-oxidizing enzyme is added to be fermented in 1000g fermentation liquid.
2) step 1) fermentation after the completion of, filter out the solidss in fermentation liquid, and at a temperature of 45 DEG C using decompression steam
The mode evaporating carries out cryoconcentration to fermentation liquid, and the concentration after concentration is 15%.
3), in fermentation liquid after concentration, add the weight than the gelatin for 1.5 ‰ and fermentation liquid with the weight of fermentation liquid
Than the cyclodextrin for 1 ‰, with fermentation liquid weight than the sodium alginate for 0.5 ‰, with the weight of fermentation liquid than the poly- second two for 7 ‰
Alcohol 4000, stirring and dissolving is uniformly divided using high speed shear afterwards and is spread mulser and carry out emulsifying, is finally spray-dried and makes sulfide-oxidizing enzyme
Microcapsule microbial agent.
Embodiment 4：
Present embodiments provide a kind of preparation method of the sulfide-oxidizing enzyme microcapsule microbial agent for water purification, walk including following
Suddenly：
1) 0.35g sulfide-oxidizing enzyme is added to be fermented in 1000g fermentation liquid.
2) step 1) fermentation after the completion of, filter out the solidss in fermentation liquid, and at a temperature of 45 DEG C using decompression steam
The mode evaporating carries out cryoconcentration to fermentation liquid, and the concentration after concentration is 15%.
3), in fermentation liquid after concentration, add the weight than the gelatin for 1.6 ‰ and fermentation liquid with the weight of fermentation liquid
Than the cyclodextrin for 1 ‰, with fermentation liquid weight than the sodium alginate for 0.4 ‰, with the weight of fermentation liquid than the poly- second for 7.5 ‰
Glycol 4000, stirring and dissolving is uniformly divided using high speed shear afterwards and is spread mulser and carry out emulsifying, is finally spray-dried that to make sulfuration thin
Strain microcapsule microbial agent.
Present invention also offers a kind of sulfide-oxidizing enzyme microcapsule microbial agent for water purification, made using above-mentioned preparation method,
Its storage time is long, easily disperses in water, meet water can disintegrate uniformly discharge sulfide-oxidizing enzyme rapidly, there is higher purification and imitate
Rate.
The only illustration to the present invention exemplified as above, does not constitute the restriction to protection scope of the present invention, all
It is that same or analogous design belongs within protection scope of the present invention with the present invention.
Claims (8)
1. a kind of sulfide-oxidizing enzyme microcapsule microbial agent for water purification it is characterised in that：Fermentation including microcapsule and sulfide-oxidizing enzyme
Liquid, described microcapsule includes the following component based on fermentation liquid percentage by weight：1.5 ‰～1.7 ‰ gelatin, 0.9 ‰～
1.2 ‰ cyclodextrin, 0.3 ‰～0.5 ‰ sodium alginate, 5 ‰～7.5 ‰ Macrogol 4000.
2. be used for as claimed in claim 1 the sulfide-oxidizing enzyme microcapsule microbial agent of water purification it is characterised in that：Described microcapsule includes
Following component based on fermentation liquid percentage by weight：1.5 ‰～1.6 ‰ gelatin, 0.9 ‰～1.0 ‰ cyclodextrin, 0.3 ‰
～0.4 ‰ sodium alginate, 7 ‰～7.5 ‰ Macrogol 4000.
3. be used for as claimed in claim 1 the sulfide-oxidizing enzyme microcapsule microbial agent of water purification it is characterised in that：Described microcapsule includes
Following component based on fermentation liquid percentage by weight：1.5 ‰ gelatin, 1.0 ‰ cyclodextrin, 0.5 ‰ sodium alginate, 7 ‰
Macrogol 4000.
4. the preparation method of the sulfide-oxidizing enzyme microcapsule microbial agent for water purification as described in a kind of any one as claims 1 to 3, its
It is characterised by：Comprise the steps：
1) sulfide-oxidizing enzyme is added to be fermented in fermentation liquid；
2) step 1) filter out solidss in fermentation liquid after the completion of fermentation, cryoconcentration is carried out to fermentation liquid；
3), in fermentation liquid after concentration, gelatin, cyclodextrin, sodium alginate, Macrogol 4000 are added, after stirring and dissolving is uniform
Through emulsifying, it is spray-dried and makes sulfide-oxidizing enzyme microcapsule microbial agent.
5. be used for as claimed in claim 4 the preparation method of the sulfide-oxidizing enzyme microcapsule microbial agent of water purification it is characterised in that：Described
Step 1) in the mass ratio of sulfide-oxidizing enzyme and fermentation liquid be 0.3 ‰～0.4 ‰.
6. be used for as claimed in claim 4 the preparation method of the sulfide-oxidizing enzyme microcapsule microbial agent of water purification it is characterised in that：Described
Step 2) in cryoconcentration temperature be 40～50 DEG C.
7. be used for as claimed in claim 4 the preparation method of the sulfide-oxidizing enzyme microcapsule microbial agent of water purification it is characterised in that：Described
Step 2) in cryoconcentration adopt vacuum distillation, the concentration after concentration be 10～20%.
8. be used for as claimed in claim 4 the preparation method of the sulfide-oxidizing enzyme microcapsule microbial agent of water purification it is characterised in that：Described
Step 3) in emulsion process carry out in high speed shear dispersion emulsifying machine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610764500.1A CN106434617A (en) | 2016-08-30 | 2016-08-30 | Thiobacillus microcapsule inoculant used for water purification and preparing method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610764500.1A CN106434617A (en) | 2016-08-30 | 2016-08-30 | Thiobacillus microcapsule inoculant used for water purification and preparing method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116211823A (en) * | 2022-12-16 | 2023-06-06 | 安徽济生元药业有限公司 | Preparation method of antioxidant lycopene capsule |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104986867A (en) * | 2015-06-23 | 2015-10-21 | 四川省畜牧科学研究院 | Complex microbial inoculant for improving aquaculture pond bottom mud environment and preparation method and application thereof |
-
2016
- 2016-08-30 CN CN201610764500.1A patent/CN106434617A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104986867A (en) * | 2015-06-23 | 2015-10-21 | 四川省畜牧科学研究院 | Complex microbial inoculant for improving aquaculture pond bottom mud environment and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 冬生等主编: "《食品高新技术》", 30 November 2007, 中国计量出版社 * |
| 谢岩黎主编: "《现代食品工程技术》", 31 December 2011, 郑州大学出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116211823A (en) * | 2022-12-16 | 2023-06-06 | 安徽济生元药业有限公司 | Preparation method of antioxidant lycopene capsule |
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Application publication date: 20170222 |