CN106434524B - A kind of Isolated Microspore in Chinese Cabbage Fiber differentiation based formulas - Google Patents
A kind of Isolated Microspore in Chinese Cabbage Fiber differentiation based formulas Download PDFInfo
- Publication number
- CN106434524B CN106434524B CN201610873780.XA CN201610873780A CN106434524B CN 106434524 B CN106434524 B CN 106434524B CN 201610873780 A CN201610873780 A CN 201610873780A CN 106434524 B CN106434524 B CN 106434524B
- Authority
- CN
- China
- Prior art keywords
- chinese cabbage
- microspore
- isolated microspore
- isolated
- vitamin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 title claims abstract description 66
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 title claims abstract description 66
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 title claims abstract description 66
- 230000004069 differentiation Effects 0.000 title description 10
- 239000000835 fiber Substances 0.000 title description 9
- 239000001963 growth medium Substances 0.000 claims abstract description 20
- 239000002609 medium Substances 0.000 claims abstract description 16
- 241000196324 Embryophyta Species 0.000 claims abstract description 15
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims abstract description 14
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims abstract description 12
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims abstract description 12
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims abstract description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 10
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 8
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 8
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 claims abstract description 8
- RWSXRVCMGQZWBV-PHDIDXHHSA-N L-Glutathione Natural products OC(=O)[C@H](N)CCC(=O)N[C@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-PHDIDXHHSA-N 0.000 claims abstract description 7
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 claims abstract description 7
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims abstract description 7
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims abstract description 7
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 claims abstract description 6
- 239000004471 Glycine Substances 0.000 claims abstract description 6
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229930182816 L-glutamine Natural products 0.000 claims abstract description 6
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 claims abstract description 6
- 235000019152 folic acid Nutrition 0.000 claims abstract description 6
- 239000011724 folic acid Substances 0.000 claims abstract description 6
- 229960000304 folic acid Drugs 0.000 claims abstract description 6
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229960001669 kinetin Drugs 0.000 claims abstract description 6
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 6
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 6
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 6
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 claims abstract description 5
- 239000007836 KH2PO4 Substances 0.000 claims abstract description 5
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims abstract description 5
- ZRKWMRDKSOPRRS-UHFFFAOYSA-N N-Methyl-N-nitrosourea Chemical compound O=NN(C)C(N)=O ZRKWMRDKSOPRRS-UHFFFAOYSA-N 0.000 claims abstract description 5
- 229910004619 Na2MoO4 Inorganic materials 0.000 claims abstract description 5
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims abstract description 5
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims abstract description 5
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 5
- 229930006000 Sucrose Natural products 0.000 claims abstract description 5
- 229930003451 Vitamin B1 Natural products 0.000 claims abstract description 5
- 235000020958 biotin Nutrition 0.000 claims abstract description 5
- 239000011616 biotin Substances 0.000 claims abstract description 5
- 229960002685 biotin Drugs 0.000 claims abstract description 5
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims abstract description 5
- 229910000366 copper(II) sulfate Inorganic materials 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims abstract description 5
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims abstract description 5
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims abstract description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 5
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims abstract description 5
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims abstract description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 5
- 235000001968 nicotinic acid Nutrition 0.000 claims abstract description 5
- 239000011664 nicotinic acid Substances 0.000 claims abstract description 5
- 229960003512 nicotinic acid Drugs 0.000 claims abstract description 5
- 239000011684 sodium molybdate Substances 0.000 claims abstract description 5
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000005720 sucrose Substances 0.000 claims abstract description 5
- 229960003495 thiamine Drugs 0.000 claims abstract description 5
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims abstract description 5
- 235000010374 vitamin B1 Nutrition 0.000 claims abstract description 5
- 239000011691 vitamin B1 Substances 0.000 claims abstract description 5
- 229910000368 zinc sulfate Inorganic materials 0.000 claims abstract description 5
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims abstract description 5
- 239000011686 zinc sulphate Substances 0.000 claims abstract description 5
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims abstract description 4
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims abstract description 4
- 229960000367 inositol Drugs 0.000 claims abstract description 4
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 claims abstract description 4
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims abstract description 4
- 235000019158 vitamin B6 Nutrition 0.000 claims abstract description 4
- 239000011726 vitamin B6 Substances 0.000 claims abstract description 4
- 229940011671 vitamin b6 Drugs 0.000 claims abstract description 4
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims description 3
- 102000002322 Egg Proteins Human genes 0.000 claims description 3
- 108010000912 Egg Proteins Proteins 0.000 claims description 3
- 235000014103 egg white Nutrition 0.000 claims description 3
- 210000000969 egg white Anatomy 0.000 claims description 3
- 206010020649 Hyperkeratosis Diseases 0.000 abstract description 18
- 210000001161 mammalian embryo Anatomy 0.000 abstract description 10
- 108010009736 Protein Hydrolysates Proteins 0.000 abstract description 3
- 239000003531 protein hydrolysate Substances 0.000 abstract description 3
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 abstract description 2
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 abstract description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 abstract 2
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 abstract 1
- 229960002743 glutamine Drugs 0.000 abstract 1
- 229960002449 glycine Drugs 0.000 abstract 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 abstract 1
- 229960002429 proline Drugs 0.000 abstract 1
- 229960001153 serine Drugs 0.000 abstract 1
- 230000006698 induction Effects 0.000 description 17
- 238000009395 breeding Methods 0.000 description 10
- 238000000034 method Methods 0.000 description 9
- 230000001488 breeding effect Effects 0.000 description 8
- 235000013311 vegetables Nutrition 0.000 description 7
- 238000011160 research Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 241000209504 Poaceae Species 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000000654 additive Substances 0.000 description 3
- 230000000996 additive effect Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 239000013049 sediment Substances 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241000208292 Solanaceae Species 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical compound [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 description 2
- MYSWGUAQZAJSOK-UHFFFAOYSA-N ciprofloxacin Chemical compound C12=CC(N3CCNCC3)=C(F)C=C2C(=O)C(C(=O)O)=CN1C1CC1 MYSWGUAQZAJSOK-UHFFFAOYSA-N 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229960002523 mercuric chloride Drugs 0.000 description 2
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- PXFBZOLANLWPMH-UHFFFAOYSA-N 16-Epiaffinine Natural products C1C(C2=CC=CC=C2N2)=C2C(=O)CC2C(=CC)CN(C)C1C2CO PXFBZOLANLWPMH-UHFFFAOYSA-N 0.000 description 1
- 241001290610 Abildgaardia Species 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 235000005637 Brassica campestris Nutrition 0.000 description 1
- 241001301148 Brassica rapa subsp. oleifera Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 229940045719 antineoplastic alkylating agent nitrosoureas Drugs 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 235000015177 dried meat Nutrition 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 229940064880 inositol 100 mg Drugs 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000005201 scrubbing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229940033203 vitamin b6 0.5 mg Drugs 0.000 description 1
- 238000004073 vulcanization Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0025—Culture media for plant cell or plant tissue culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/35—Polyols, e.g. glycerin, inositol
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/40—Nucleotides, nucleosides or bases
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides a kind of Isolated Microspore in Chinese Cabbage induced medium, formula contains KNO3、MgSO4∙7H2O、KH2PO4、Ca(NO3)2∙4H2O、Na2‑EDTA、FeSO4∙7H2O、MnSO4∙4H2O、AgNO3、H3BO3、ZnSO4∙7H2O、KI、Na2MoO4∙2H2O、CuSO4∙5H2O、CoCl2∙6H2O, L-Glutamine, vitamin B1, vitamin B6, niacin, folic acid, biotin, inositol, glycine, proline, serine, l-Glutathione, methyl-nitroso-urea, sucrose, plant gel, 6-BA, multiple phthalein nucleic acid, protein hydrolysate, colchicin, active carbon and plant vulcanize kinetin PSK- α.The culture medium can greatly improve the induced efficiency of Isolated Microspore in Chinese Cabbage embryo callus, have important application value.
Description
Technical field
The present invention relates to a kind of Isolated Microspore in Chinese Cabbage Fiber differentiation based formulas, and in particular to one kind can greatly improve
Isolated Microspore in Chinese Cabbage induction is the culture medium prescription of embryo callus efficiency, belongs to vegetable cultivation high-technology field.
Background technique
Chinese cabbage (Brassica campestris ssp. pekinensisLour.) belong to Cruciferae Brassica genus rue
A kind of sedge kind Chinese cabbage subspecies are a kind of important vegetables for originating in China.Chinese cabbage ecotype multiplicity, distribution is wide, yield
Height, storage tolerance, the supply phase is long, and nutrition is comprehensive, eating method multiplicity, and plants simple, saving of labor, at low cost, in China's vegetable basket
It is middle to occupy irreplaceable critical role.The cultivated area and yield of China Chinese cabbage are Chinese cabbage first of various vegetable crops
Production occupies leading position in vegetable production.Due to history, ecology, production and consumption habit difference, northern China
Area is the main producing region of Chinese cabbage.
With the improvement of people's life quality, to Chinese cabbage quality, higher requirements are also raised, and just there is an urgent need to educate for this
Kind worker selects more high-quality, disease-resistant, high yield excellent variety.Chinese cabbage is cross-pollinatd plant, is had significant
Hybrid vigour mainly carries out New Chinese Cabbage Variety cultivation using cross breeding method at present.Conventional hybridization breeding method is bred as
A series of Chinese cabbage cultivars are made that brilliant contribution for vegetables production.However, there are the periods for Chinese cabbage crossing breeding method
The outstanding problems such as length, low efficiency are bred as a stable Elite inbred and generally require 6-8, or even longer time, breeding selfing
System is very bothersome, takes a lot of work.Recently as the increase of cost of labor, the deficiency of conventional breeding is increasingly obvious in actual operation.
Therefore, scholars begin look for the new way and new method of breeding.
Microspore-isolated culture refers to that free, fresh microspore group is directly obtained from bud or flower medicine to carry out
Culture, via the induction of embryoid or callus, regenerates complete haplobiont, using artificial or Natural double,
Technology as normal fertile, homozygous liploid plant.Microspore-isolated culture has pure haploidy, unicellular property and culture
The features such as cell quantity is big, therefore increasingly by the favor of breeder.The technology can be obtained for breeding in 1-2
Elite inbred and the not affine pure lines of selfing, therefore it is substantially shorter the breeding time limit, to improve breeding efficiency.Isolated microspore
Culture technique, more successful research field is grass family and Solanaceae at present, and a variety of grass family and solanaceous crops are proved to be successful
Efficient induction and differentiated system out.The isolated microspore culture technique of brassicaceous vegetable has also carried out a series of researchs, right
The embryogenetic mechanism of Chinese cabbage microspore, influence factor have done a large amount of research and probe, and achieve many important researchs
Progress.
The Isolated Microspore in Chinese Cabbage culture studies in China start from the late 1980s.1988, agriculture section, Henan Province
Institute Horticultural Research Institute Li Genyi etc. at home for the first time induces Isolated Microspore in Chinese Cabbage at embryo and embryo reproductive success.Then, river
The technology is applied to the culture of DH pure lines and parent material by the academy of agricultural sciences Nan Sheng, has successfully been bred as Henan Chinese cabbage No. 7, has been become domestic and international
The new varieties that first Isolated Microspore in Chinese Cabbage cultural method is cultivated.Later, and successively 14 are selected by above the provincial level
Examine (mirror) fixed different type New Chinese Cabbage Variety, wherein Henan Chinese cabbage No. 7 between 1994-1997 popularizing area reach 3.01 ten thousand
hm2.The Vegetable Research center of Beijing in 2002 is special using the Exocarpium Citri Rubrum heart kind acquisition country invention that microspore culture is bred as
Benefit.Although Isolated Microspore in Chinese Cabbage culture breeding technique achieves a series of research progress, Henan Chinese cabbage No. 7, Henan are also cultivated
New No. 3, Yuyuan Garden 5, the excellent variety such as the Exocarpium Citri Rubrum heart, but Isolated Microspore Culture in Chinese cabbage and not perfect, mainly greatly
The problems such as Chinese cabbage Isolated microspore inductivity is not high, and embryoid planting percent is low is never well solved.Microspore lures
Low will lead to of conductance is difficult to obtain enough selection groups, directly limits Isolated Microspore Culture in Chinese cabbage in breeding
Application.
Culture medium is the material base of microspore-isolated culture, is directly related to the growth and differentiation of microspore, culture medium
Stimulation and starting of the component to microspore, are the factors that can Isolated microspore induce successful most critical.Nutrient media components
Appropriate proportioning, can significantly affect microspore induced efficiency, and some organic and inorganic additive can significantly improve microspore induction
Efficiency.Studies have found that, relative to grass family and solanaceous crops, the minimal medium formula of low ion concns is to cruciate flower at present
Section microspore induces advantageously, and amino acid and active carbon can significantly improve the inducibility of Isolated Microspore in Chinese Cabbage.Cause
This, continues optimum organization minimal medium proportion, excavates and test is directed to and improves Isolated Microspore in Chinese Cabbage into embryonal induction energy
The organic-inorganic additive of power is the key that establish the efficient Fiber differentiation system of Isolated Microspore in Chinese Cabbage.
Summary of the invention
The object of the present invention is to provide the inductions that one kind can greatly improve Isolated Microspore in Chinese Cabbage embryo callus
Efficiency, the Isolated Microspore in Chinese Cabbage Fiber differentiation based formulas with important application value.
The purpose of the present invention is what is solved by the following technical programs:
A kind of Isolated Microspore in Chinese Cabbage Fiber differentiation based formulas, it is characterised in that: the formula of the culture medium is as follows:
KNO356-64mg/L, MgSO4∙7H2O 60-65mg/L, KH2PO465-70mg/L, Ca (NO3)2∙4H2O 200-
250mg/L, Na2- EDTA 11-14mg/L, FeSO4∙7H2O 12-15mg/L, MnSO4∙4H2O 23-27mg/L, AgNO3 7-
9mg/L, H3BO38.0-8.5mg/L, ZnSO4∙7H2O 8.5-9.5mg/L, KI 0.4-0.45mg/L, Na2MoO4∙2H2O
0.2-0.3mg/L, CuSO4∙5H2O 0.02-0.03mg/L, CoCl2∙6H2O 0.02-0.03mg/L, L-Glutamine 750-
850mg/L, vitamin B1 0.45-0.55mg/L, vitamin B6 0.45-0.55mg/L, niacin 4.5-5.5mg/L, folic acid
0.04-0.06mg/L, biotin 0.04-0.06mg/L, inositol 90-110mg/L, glycine 3.5-4.5mg/L, proline
17-23mg/L, serine 70-90mg/L, l-Glutathione 27-33mg/L, methyl-nitroso-urea 1.2-1.8g/L, sucrose
120-140g/L, plant gel 3.0-3.5g/L, 6-BA 0.2-0.3mg/L, multiple phthalein nucleic acid 0.15-0.25mg/L, hydrolyze egg
White 0.5-0.7g/L, colchicin 0.06-0.08mg/L, active carbon 0.04-0.06g/L, plant vulcanize kinetin PSK- α
30-40pmol/L, pH 5.6-5.8.
The present invention has the following advantages compared with prior art:
The present invention is directed to the spore of Isolated Microspore in Chinese Cabbage according to grass family and Solanaceae microspore Fiber differentiation based formulas
Daughter developmental characteristic continues to optimize and combine minimal medium proportion, and low ion concns minimal medium assembles, and is conducive to big
The induction of Chinese cabbage Isolated microspore；Using the plant gel of low content, make the culture medium semisolid being configured to, Chinese cabbage is swum
It is more advantageous from the induction of microspore；It excavates and test is directed to raising Isolated Microspore in Chinese Cabbage and adds at the organic of embryonal induction ability
Add object and inorganic additive, protein hydrolysate, L-Glutamine, folic acid, glycine, proline, serine, l-Glutathione, first
The addition of the suitable concentrations of substances such as base nitroso ureas, colchicin, active carbon, plant vulcanization kinetin PSK- α, difference journey
The inductivity for improving Isolated Microspore in Chinese Cabbage of degree；The compounding of 6-BA and multiple phthalein nucleic acid, it is free to significantly improve Chinese cabbage
The inducibility of microspore.
Isolated Microspore in Chinese Cabbage induced medium provided by the present invention is now studies have found that enterprising the one of basis
The achievement of the creative improvement of step, is the optimum combination screened by a large amount of single-factors, multifactor experiment, We conducted a large amount of
Experimental study also show that component proportion of the invention is optimal.It can be greatly improved using culture medium provided by the present invention
The induced efficiency of free Chinese cabbage microspore embryo callus, therefore industry promotional value with higher.
Specific embodiment
Below with reference to embodiment, the present invention is further illustrated.
A kind of Isolated Microspore in Chinese Cabbage Fiber differentiation based formulas, it is characterised in that: the formula of the culture medium is as follows:
KNO356-64mg/L, MgSO4∙7H2O 60-65mg/L, KH2PO465-70mg/L, Ca (NO3)2∙4H2O 200-
250mg/L, Na2- EDTA 11-14mg/L, FeSO4∙7H2O 12-15mg/L, MnSO4∙4H2O 23-27mg/L, AgNO3 7-
9mg/L, H3BO38.0-8.5mg/L, ZnSO4∙7H2O 8.5-9.5mg/L, KI 0.4-0.45mg/L, Na2MoO4∙2H2O
0.2-0.3mg/L, CuSO4∙5H2O 0.02-0.03mg/L, CoCl2∙6H2O 0.02-0.03mg/L, L-Glutamine 750-
850mg/L, vitamin B1 0.45-0.55mg/L, vitamin B6 0.45-0.55mg/L, niacin 4.5-5.5mg/L, folic acid
0.04-0.06mg/L, biotin 0.04-0.06mg/L, inositol 90-110mg/L, glycine 3.5-4.5mg/L, proline
17-23mg/L, serine 70-90mg/L, l-Glutathione 27-33mg/L, methyl-nitroso-urea 1.2-1.8g/L, sucrose
120-140g/L, plant gel 3.0-3.5g/L, 6-BA 0.2-0.3mg/L, multiple phthalein nucleic acid 0.15-0.25mg/L, hydrolyze egg
White 0.5-0.7g/L, colchicin 0.06-0.08mg/L, active carbon 0.04-0.06g/L, plant vulcanize kinetin PSK- α
30-40pmol/L, pH 5.6-5.8.
In the Chinese cabbage flowering stage in 4-5 month, the bud of 2.2mm-3.0mm is taken to take back laboratory, is placed in 4 DEG C of refrigerators
Cold pretreatment 1-2d.Before inoculation, bud is transferred in superclean bench sterile beaker, 70% alcohol progress surface is poured into and disappears
Poison outwells alcohol after impregnating 1min, adds 0.1% mercuric chloride solution soaking disinfection 10min, then three times with aseptic water washing
Sufficiently to remove mercury chloride residual hazard.After draining away the water, bud is rolled with tack glass rod in B5 liquid scrubbing culture medium, is squeezed out small
Spore.Suspension collects filtrate and is centrifuged 3min under 10ml centrifuge tube, 1000rpm, precipitate through 30 μm of sterile micropore filtered through gauze
Object adds 5mL B5 to wash culture medium, shakes up, is centrifuged 2min under 800rpm, is repeated twice, and gained sediment is that Chinese cabbage is free
Microspore.Isolated Microspore in Chinese Cabbage is transferred in Isolated Microspore in Chinese Cabbage induced medium of the invention, 33 DEG C of heat shocks
Processing is transferred to 25 DEG C of dark cultures afterwards for 24 hours until embryo callus is formed.
Embodiment
A kind of Isolated Microspore in Chinese Cabbage induced medium of the present invention is prepared, culture medium prescription is specific as follows:
KNO360mg/L, MgSO4∙7H2O 62.5mg/L, KH2PO467.5mg/L, Ca (NO3)2∙4H2O 225mg/L,
Na2- EDTA 12.5mg/L, FeSO4∙7H2O 13.5mg/L, MnSO4∙4H2O 25mg/L, AgNO38mg/L, H3BO3
8.25mg/L ZnSO4∙7H2O 9.0mg/L, KI 0.425mg/L, Na2MoO4∙2H2O 0.25mg/L, CuSO4∙5H2O
0.025mg/L, CoCl2∙6H2O 0.025mg/L, L-Glutamine 800mg/L, vitamin B1 0.5mg/L, vitamin B6
0.5mg/L, niacin 5mg/L, folic acid 0.05mg/L, biotin 0.05mg/L, inositol 100mg/L, glycine 4mg/L, dried meat
Propylhomoserin 20mg/L, serine 80mg/L, l-Glutathione 30mg/L, methyl-nitroso-urea 1.5g/L, sucrose 130g/L,
Plant gel 3.25g/L, 6-BA 0.25mg/L, multiple phthalein nucleic acid 0.2mg/L, protein hydrolysate 0.6g/L, colchicin
0.07mg/L, active carbon 0.05g/L, plant vulcanize kinetin PSK- α 35pmol/L, pH 5.7.
In April, 2015 in white No. 17 of Chinese cabbage cultivar Shandong and all-victorious flowering stage, takes the Shandong of 2.2mm-3.0mm white 17
Number and all-victorious bud take back laboratory, be placed in Cold pretreatment 2d in 4 DEG C of refrigerators.Before inoculation, bud is transferred to ultra-clean work
In platform sterile beaker, pours into 70% alcohol and carry out surface sterilization, outwell alcohol after impregnating 1min, add 0.1% mercuric chloride solution
Soaking disinfection 10min, then with aseptic water washing three times sufficiently to remove mercury chloride residual hazard.After draining away the water, washed in B5 liquid
It washs in culture medium and rolls bud with tack glass rod, squeeze out microspore.Suspension collects filtrate through 30 μm of sterile micropore filtered through gauze
3min is centrifuged under 10ml centrifuge tube, 1000rpm, sediment adds 5mL B5 to wash culture medium, shakes up, be centrifuged under 800rpm
2min is repeated twice, and gained sediment is Isolated Microspore in Chinese Cabbage.By white No. 17 of Shandong and all-victorious Isolated microspore difference
It is transferred in Isolated Microspore in Chinese Cabbage induced medium of the invention, 33 DEG C of Heat thermostabilities are transferred to 25 DEG C of dark cultures, embryo afterwards for 24 hours
Property callus formed after count white No. 17 of Shandong and all-victorious microspore callus induction rate respectively.Microspore callus lures
Conductance=callus block number/inoculation bud sum × 100%.
The culture medium pair used for Isolated Microspore in Chinese Cabbage induced medium more provided by the present invention and forefathers
The difference of Isolated Microspore in Chinese Cabbage embryonic callus induction efficiency, in addition we have selected 2 kinds of forefathers to adopt used great Bai
Dish Isolated microspore induced medium, test variety also use Shandong white No. 17 and all-victorious, the separation method of Isolated microspore, tissue culture
Operating method, cultural method are all the same, embryo callus counted respectively after being formed white No. 17 of Shandong and all-victorious microspore this 2
Microspore callus induction rate in kind culture medium.
Other 2 kinds of Isolated Microspore in Chinese Cabbage Fiber differentiation based formulas are as follows:
NLH minimal medium+30mg/L glutathione+100mg/L serine+800mg/L glutamine+0.4mg/L 6-
BA, pH are 5.8(documents from Han Yang etc. " Chinese cabbage microspores culture Study on influencing factors ")；
NLN-13 culture medium+0.1g/L active carbon, pH are 5.8(documents from " the small robe training of Chinese cabbage such as Sun Dan
Support regeneration strain and its otherness preliminary analysis ").
Culture medium comparative experiments
It will be using white No. 17 of culture medium provided by the present invention induction Shandong and all-victorious Isolated microspore statistics gained microspore
Callus induction rate and the Shandong of comparative example white No. 17 and all-victorious microspore callus induction rate are compared, and are compared
As a result as shown in table 1 below.
By table 1 it can be found that Isolated Microspore in Chinese Cabbage induced medium provided by the present invention is to Chinese cabbage cultivar Shandong
White No. 17 have reached 30.2/flower bud with all-victorious microspore callus induction rate average value, and two kinds of trainings used by forefathers
Support No. 17 white to Chinese cabbage cultivar Shandong of base and all-victorious microspore callus induction rate average value respectively and be only 9.2/flower bud and
13.0/flower bud, it can be found that Isolated Microspore in Chinese Cabbage induced medium provided by the present invention is to Isolated Microspore in Chinese Cabbage
Inducibility be made that and be obviously improved.
Isolated Microspore in Chinese Cabbage induced medium provided by the present invention is now studies have found that enterprising the one of basis
The achievement of the creative improvement of step, is the optimum combination screened by a large amount of single-factors, multifactor experiment, We conducted a large amount of
Experimental study also show that component proportion of the invention is optimal.It can be greatly improved using culture medium provided by the present invention
The induced efficiency of free Chinese cabbage microspore embryo callus, therefore industry promotional value with higher.
The above examples only illustrate the technical idea of the present invention, and this does not limit the scope of protection of the present invention, all
According to the technical idea provided by the invention, any changes made on the basis of the technical scheme each falls within the scope of the present invention
Within；The technology that the present invention is not directed to can be realized by the prior art.
Claims (1)
1. a kind of Isolated Microspore in Chinese Cabbage induced medium, it is characterised in that: the formula of the culture medium is as follows:
KNO356-64mg/L, MgSO4∙7H2O 60-65mg/L, KH2PO465-70mg/L, Ca (NO3)2∙4H2O 200-
250mg/L, Na2- EDTA 11-14mg/L, FeSO4∙7H2O 12-15mg/L, MnSO4∙4H2O 23-27mg/L, AgNO3 7-
9mg/L, H3BO38.0-8.5mg/L, ZnSO4∙7H2O 8.5-9.5mg/L, KI 0.4-0.45mg/L, Na2MoO4∙2H2O
0.2-0.3mg/L, CuSO4∙5H2O 0.02-0.03mg/L, CoCl2∙6H2O 0.02-0.03mg/L, L-Glutamine 750-
850mg/L, vitamin B1 0.45-0.55mg/L, vitamin B6 0.45-0.55mg/L, niacin 4.5-5.5mg/L, folic acid
0.04-0.06mg/L, biotin 0.04-0.06mg/L, inositol 90-110mg/L, glycine 3.5-4.5mg/L, proline
17-23mg/L, serine 70-90mg/L, l-Glutathione 27-33mg/L, methyl-nitroso-urea 1.2-1.8g/L, sucrose
120-140g/L, plant gel 3.0-3.5g/L, 6-BA 0.2-0.3mg/L, multiple phthalein nucleic acid 0.15-0.25mg/L, hydrolyze egg
White 0.5-0.7g/L, colchicin 0.06-0.08mg/L, active carbon 0.04-0.06g/L, plant vulcanize kinetin PSK- α
30-40pmol/L, pH 5.6-5.8；The kind of the Chinese cabbage is Shandong white No. 17 and complete victory.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610873780.XA CN106434524B (en) | 2016-10-08 | 2016-10-08 | A kind of Isolated Microspore in Chinese Cabbage Fiber differentiation based formulas |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610873780.XA CN106434524B (en) | 2016-10-08 | 2016-10-08 | A kind of Isolated Microspore in Chinese Cabbage Fiber differentiation based formulas |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106434524A CN106434524A (en) | 2017-02-22 |
| CN106434524B true CN106434524B (en) | 2019-06-18 |
Family
ID=58171584
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610873780.XA Expired - Fee Related CN106434524B (en) | 2016-10-08 | 2016-10-08 | A kind of Isolated Microspore in Chinese Cabbage Fiber differentiation based formulas |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106434524B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434524B (en) * | 2016-10-08 | 2019-06-18 | 安徽轩之界农业发展有限公司 | A kind of Isolated Microspore in Chinese Cabbage Fiber differentiation based formulas |
| CN107164303B (en) * | 2017-07-19 | 2020-11-20 | 泗县微腾知识产权运营有限公司 | Pumpkin isolated microspore induction culture medium and induction culture method |
| CN108184677A (en) * | 2018-04-02 | 2018-06-22 | 张瑞明 | A kind of pakchoi Isolated microspore Fiber differentiation based formulas |
| CN108849520A (en) * | 2018-08-07 | 2018-11-23 | 江苏高航农业科技有限公司 | A kind of woaded blue haploid breeding flower training induced medium and its preparation method |
| CN109644873B (en) * | 2019-02-01 | 2021-11-09 | 云南省农业科学院甘蔗研究所 | Sugarcane tropical seed embryogenic cell mass induction method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101433184A (en) * | 2007-11-15 | 2009-05-20 | 刘祥军 | Culture medium prescription of regenerated plant from isolated microspore of brassica |
| CN106434524A (en) * | 2016-10-08 | 2017-02-22 | 宋兆霞 | Chinese cabbage isolated microspore inducing medium formula |
-
2016
- 2016-10-08 CN CN201610873780.XA patent/CN106434524B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101433184A (en) * | 2007-11-15 | 2009-05-20 | 刘祥军 | Culture medium prescription of regenerated plant from isolated microspore of brassica |
| CN106434524A (en) * | 2016-10-08 | 2017-02-22 | 宋兆霞 | Chinese cabbage isolated microspore inducing medium formula |
Non-Patent Citations (1)
| Title |
|---|
| 甘蓝类蔬菜游离小抱子培养研究进展;汤青林等;《西南农业学报》;20001231;第13卷(第3期);98-103 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106434524A (en) | 2017-02-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106434524B (en) | A kind of Isolated Microspore in Chinese Cabbage Fiber differentiation based formulas | |
| CN103583369B (en) | Induction medium for culturing callus of barley microspore | |
| CN105900845B (en) | Somatic embryo rapid propagation seedling raising method for camellia chekiangoleosa | |
| Afza et al. | Effect of spikelet position on rice anther culture efficiency | |
| JP2018535672A (en) | Polyploid rice light and temperature sensitive gene male sterility system and its breeding method | |
| CN106258984B (en) | A kind of Chinese cabbage microspore differential medium formula | |
| CN106258983A (en) | A kind of Plantula Brassicae chinensis Isolated microspore inducing culture based formulas | |
| Zeng et al. | Reduced ascorbate and reduced glutathione improve embryogenesis in broccoli microspore culture | |
| CN110250007A (en) | A kind of culture medium broken up again for peanut microspore callus | |
| CN103416304A (en) | Method for cultivating water-saving and drought-resistant rice anther | |
| CN101773072B (en) | Method for culturing isolated microspore of common head cabbage to obtain regeneration plant | |
| CN110506635B (en) | Marigold pollen induction culture medium and induction culture method | |
| CN104719169A (en) | Formula of induction medium for efficiently inducing anther culture of japonica rice | |
| CN106069768A (en) | A kind of Anthers of Hordeum Vulgare method for inducing and cultivating | |
| CN109042336A (en) | With the method for the sterile callus of rice paddy seed culture rice containing endogenous bacterium | |
| CN103053413A (en) | Chemical corn double haploid young embryo processing method | |
| CN108901827A (en) | Japonica hybrid rice sterile line breeding method when a kind of early blossoming | |
| CN101049083A (en) | Method for breeding distinguished germ plasm of high-grade triticale rich in chrome | |
| CN107347627A (en) | A kind of method of the blue sexual polyploid of efficiently founder | |
| CN105638480B (en) | A kind of capsicum variety, which is cultivated, uses flower pesticide Fiber differentiation based formulas | |
| CN105961200A (en) | Tobacco anther differential medium and preparation method | |
| CN106520659B (en) | A kind of cabbage pollen induction culture medium prescription | |
| CN109197580A (en) | A kind of efficient selection of good quality and high output cotton | |
| CN104705189B (en) | A kind of japonica rice flower pesticide inducing culture based formulas | |
| CN110622850B (en) | Sexual reproduction artificial crossbreeding method for fine purified variety and wild variety of sargassum fusiforme |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20190521 Address after: 236509 East 01 Line 204, Weizhuang Village, Jinzhai Township, Jieshou City, Fuyang City, Anhui Province Applicant after: Anhui Xuanzhijie Agricultural Development Co.,Ltd. Address before: 276309 Fu Wang Shan village, Zhang Zhuang Town, Yinan County, Linyi, Shandong Applicant before: Song Zhaoxia |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190618 Termination date: 20211008 |