CN106434520A - Preparation method of bacillus spores - Google Patents
Preparation method of bacillus spores Download PDFInfo
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- CN106434520A CN106434520A CN201610856595.XA CN201610856595A CN106434520A CN 106434520 A CN106434520 A CN 106434520A CN 201610856595 A CN201610856595 A CN 201610856595A CN 106434520 A CN106434520 A CN 106434520A
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Abstract
The invention relates to a preparation method of bacillus spores. The preparation method comprises the following steps: inoculating 1 part of bacillus stearothermophilus into 10 parts of a nutrient-rich culture medium, and carrying out culturing for 12h at the temperature of 50-55 DEG C and the speed of 150-250 rpm/min; adding with 90 parts of the nutrient-rich culture medium, and carrying out culturing for 12h, thus obtaining nutritive cell culture fluid; adding 1 part of an inorganic ion culture medium to 1 part of the nutritive cell culture fluid, and carrying out culturing for 12h at the temperature of 55-60 DEG C and the speed of 100-150 rpm/min; and adding 1 part of the inorganic ion culture medium every 12h till the ratio of the total parts of the added inorganic ion culture medium to the nutrient-rich culture medium is 7:1 to 10:1, thus obtaining the culture fluid containing the bacillus spores. According to the scheme, only two culture media are needed, the operation is simple, and the repeatability is good; the culture time is shortened, and the consumed time is only 60-72 h; the yield of the spores achieves 10<9>/ml, and the spore rate is 90% or above.
Description
Technical field
The present invention relates to a kind of microbial spores preparation field is and in particular to a kind of preparation method of bacillus spore.
Background technology
Bacstearothermophilus are gram positive bacteria, aerobic or amphimicrobian, and bacstearothermophilus can produce spore,
Optimum growth temp is 55-60 DEG C, and maximum growth temperature is 65-75 DEG C, belongs to Thermophilic Bacteria.Chinese industrial Microbiological Culture Collection
Administrative center (CICC) collection numbering is CICC：10267；Or ATCC：7953.
Bacstearothermophilus produced spore no pathogenicity, apyrogeneity, nontoxic.This spore has really to certain sterilizing factor
Fixed resistance, therefore can be used to monitor sterilization effect as indicator microoraganism, including pressuresteam sterilization, hydrogen peroxide
(H2O2) sterilization method such as Low-temperature Plasma Sterilization, low-temperature formaldehyde vapor sterilizing.As sterile biological indicator bacteria, according to China
Pharmacopeia code requirement, Number of spores generally 5 × 105To 5 × 106Individual, statoblast content need to account for more than 90%.
At present, it is already possible to realize the spore of bacstearothermophilus is carried out with the preparation of low dose in prior art,
But in scale of mass production, but there are many technology barriers, be primarily present that spore forming rate is not high, production cost is excessively high asks
Topic, time-consuming simultaneously, and technique is loaded down with trivial details.
Content of the invention
In place of the deficiencies in the prior art, it is an object of the invention to proposing a kind of preparation side of bacillus spore
Method, it passes through liquid cultivating method easy and simple to handle, the principle being generated using spore, turning by two kinds of different liquids culture medium
Change, to ensure yield and the yield of spore.
For achieving the above object, technical scheme is summarized as follows：
A kind of preparation method of bacillus spore, it comprises the following steps：
Step 1) trophocyte propagation：
The bacstearothermophilus of 1 weight portion are inoculated in the eutrophy culture medium of 10 weight portions, in 50-55 DEG C of environment
In, after rotating speed is for culture 12h under the conditions of 150-250rpm/min；It is subsequently added the eutrophy culture medium of 90 weight portions, continue training
Foster 12h, obtains trophocyte culture fluid；
Step 2) spore conversion：
Add the inorganic ionss culture medium of 1 weight portion in the described trophocyte culture fluid of 1 weight portion, at 55-60 DEG C
In environment, rotating speed is culture 12h under the conditions of 100-150rpm/min；The inorganic ionss culture of 1 weight portion is subsequently added every 12h
Base, until the gross mass part of inorganic ionss culture medium adding and the mass parts ratio of eutrophy culture medium reach 7: 1-10: 1；
Obtain the culture fluid containing bacillus spore.
Preferably, the preparation method of described bacillus spore, wherein, described eutrophy culture medium includes：5-20
The soy peptone of weight portion, the beef extract of 5 weight portions, 3-5 weight portion yeast extract, the sodium chloride of 5 weight portions and
The distilled water of 1000 weight portions.
Preferably, the preparation method of described bacillus spore, wherein, the pH of described eutrophy culture medium is 7.2-
7.5.
Preferably, the preparation method of described bacillus spore, wherein, described inorganic ionss culture medium includes：1 weight
The MnSO of NaCl, 0.5-1 weight portion of amount part4·H2The MgSO of O, 0.1-0.5 weight portion4·2H2O, 0.01-1 weight portion
CaCl2·2H2The KH of O, 0.01-0.1 weight portion2PO4Distilled water with 1000 weight portions.Bacillus spore is that thalline is being disliked
The hypopuss producing under bad environment, and the effect of inorganic ionss culture medium is exactly so that nutrient substance is reduced, environment becomes severe.
Preferably, the preparation method of described bacillus spore, wherein, the pH of described inorganic ionss culture medium is
7.2-7.5.
Preferably, the preparation method of described bacillus spore, wherein, described inorganic ionss culture medium also includes
The ZnCl of 0.1-0.2 weight portion2.
Preferably, the preparation method of described bacillus spore, wherein, described inorganic ionss culture medium also includes
The FeCl of 0.1-0.2 weight portion3.
The invention has the beneficial effects as follows：This case by the improvement of existing bacillus spore preparation technology so that spore
Preparation method only needs two kinds of culture medium, and operating procedure is simple, reproducible；Incubation time is further shortened, consumption
When only need 60-72h；Spore production has reached 109Every milliliter, spore rate is more than 90%.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to description
Word can be implemented according to this.
Embodiment 1
A kind of preparation method of bacillus spore, it comprises the following steps：
Step 1) trophocyte propagation：
The bacstearothermophilus of 1g are inoculated in the eutrophy culture medium of 10g, in 50 DEG C of environment, rotating speed is
After culture 12h under the conditions of 150rpm/min；It is subsequently added the eutrophy culture medium of 90g, continue culture 12h, obtain trophocyte
Culture fluid；
Step 2) spore conversion：
Add the inorganic ionss culture medium of 1g in the trophocyte culture fluid of 1g, in 55 DEG C of environment, rotating speed is
12h is cultivated under the conditions of 100rpm/min；The inorganic ionss culture medium of 1g is subsequently added every 12h, until the inorganic ionss adding
The mass parts ratio of gross mass part of culture medium and eutrophy culture medium reaches 7: 1；Obtain the culture fluid containing bacillus spore.
Wherein, eutrophy culture medium preferably includes：The soy peptone of 5-20 weight portion, the beef extract of 5 weight portions, 3-
The distilled water of 5 weight portion yeast extracts, the sodium chloride of 5 weight portions and 1000 weight portions.The pH of eutrophy culture medium controls
7.2-7.5.
Wherein, inorganic ionss culture medium preferably includes：The MnSO of NaCl, 0.5-1 weight portion of 1 weight portion4·H2O、0.1-
The MgSO of 0.5 weight portion4·2H2The CaCl of O, 0.01-1 weight portion2·2H2The KH of O, 0.01-0.1 weight portion2PO4With 1000 weights
The distilled water of amount part.The pH of inorganic ionss culture medium controls in 7.2-7.5.
Embodiment 2
A kind of preparation method of bacillus spore, it comprises the following steps：
Step 1) trophocyte propagation：
The bacstearothermophilus of 1g are inoculated in the eutrophy culture medium of 10g, in 55 DEG C of environment, rotating speed is
After culture 12h under the conditions of 250rpm/min；It is subsequently added the eutrophy culture medium of 90g, continue culture 12h, obtain trophocyte
Culture fluid；
Step 2) spore conversion：
Add the inorganic ionss culture medium of 1g in the trophocyte culture fluid of 1g, in 60 DEG C of environment, rotating speed is
12h is cultivated under the conditions of 150rpm/min；The inorganic ionss culture medium of 1g is subsequently added every 12h, until the inorganic ionss adding
The mass parts ratio of gross mass part of culture medium and eutrophy culture medium reaches 10: 1；Obtain the culture containing bacillus spore
Liquid.
Wherein, eutrophy culture medium preferably includes：The soy peptone of 5-20 weight portion, the beef extract of 5 weight portions, 3-
The distilled water of 5 weight portion yeast extracts, the sodium chloride of 5 weight portions and 1000 weight portions.The pH of eutrophy culture medium controls
7.2-7.5.
Wherein, inorganic ionss culture medium preferably includes：The MnSO of NaCl, 0.5-1 weight portion of 1 weight portion4·H2O、0.1-
The MgSO of 0.5 weight portion4·2H2The CaCl of O, 0.01-1 weight portion2·2H2The KH of O, 0.01-0.1 weight portion2PO4With 1000 weights
The distilled water of amount part.The pH of inorganic ionss culture medium controls in 7.2-7.5.
Embodiment 3
A kind of preparation method of bacillus spore, it comprises the following steps：
Step 1) trophocyte propagation：
The bacstearothermophilus of 1g are inoculated in the eutrophy culture medium of 10g, in 52 DEG C of environment, rotating speed is
After culture 12h under the conditions of 200rpm/min；It is subsequently added the eutrophy culture medium of 90g, continue culture 12h, obtain trophocyte
Culture fluid；
Step 2) spore conversion：
Add the inorganic ionss culture medium of 1g in the trophocyte culture fluid of 1g, in 57 DEG C of environment, rotating speed is
12h is cultivated under the conditions of 125rpm/min；The inorganic ionss culture medium of 1g is subsequently added every 12h, until the inorganic ionss adding
The mass parts ratio of gross mass part of culture medium and eutrophy culture medium reaches 8.5: 1；Obtain the culture containing bacillus spore
Liquid.
Wherein, eutrophy culture medium preferably includes：The soy peptone of 5-20 weight portion, the beef extract of 5 weight portions, 3-
The distilled water of 5 weight portion yeast extracts, the sodium chloride of 5 weight portions and 1000 weight portions.The pH of eutrophy culture medium controls
7.2-7.5.
Wherein, inorganic ionss culture medium preferably includes：The MnSO of NaCl, 0.5-1 weight portion of 1 weight portion4·H2O、0.1-
The MgSO of 0.5 weight portion4·2H2The CaCl of O, 0.01-1 weight portion2·2H2The KH of O, 0.01-0.1 weight portion2PO4With 1000 weights
The distilled water of amount part.The pH of inorganic ionss culture medium controls in 7.2-7.5.
Embodiment 4
A kind of preparation method of bacillus spore, it comprises the following steps：
Step 1) trophocyte propagation：
The bacstearothermophilus of 1kg are inoculated in the eutrophy culture medium of 10kg, in 50 DEG C of environment, rotating speed is
After culture 12h under the conditions of 150rpm/min；It is subsequently added the eutrophy culture medium of 90kg, continue culture 12h, obtain trophocyte
Culture fluid；
Step 2) spore conversion：
Add the inorganic ionss culture medium of 1kg in the trophocyte culture fluid of 1kg, in 55 DEG C of environment, rotating speed is
12h is cultivated under the conditions of 100rpm/min；The inorganic ionss culture medium of 1kg is subsequently added every 12h, until the inorganic ionss adding
The mass parts ratio of gross mass part of culture medium and eutrophy culture medium reaches 7: 1；Obtain the culture fluid containing bacillus spore.
Wherein, eutrophy culture medium preferably includes：The soy peptone of 5-20 weight portion, the beef extract of 5 weight portions, 3-
The distilled water of 5 weight portion yeast extracts, the sodium chloride of 5 weight portions and 1000 weight portions.The pH of eutrophy culture medium controls
7.2-7.5.
Wherein, inorganic ionss culture medium preferably includes：The MnSO of NaCl, 0.5-1 weight portion of 1 weight portion4·H2O、0.1-
The MgSO of 0.5 weight portion4·2H2The CaCl of O, 0.01-1 weight portion2·2H2The KH of O, 0.01-0.1 weight portion2PO4With 1000 weights
The distilled water of amount part.The pH of inorganic ionss culture medium controls in 7.2-7.5.
Embodiment 5
The ZnCl of 0.1-0.2 weight portion is also included in inorganic ionss culture medium2, remaining is same as Example 1.
Embodiment 6
The FeCl of 0.1-0.2 weight portion is also included in inorganic ionss culture medium3, remaining is same as Example 5.
Comparative example 1
Rate-determining steps 2) in add gross mass part of inorganic ionss culture medium and eutrophy culture medium mass parts ratio
For 6: 1；Remaining is same as Example 1.
Comparative example 2
Rate-determining steps 2) in add gross mass part of inorganic ionss culture medium and eutrophy culture medium mass parts ratio
For 11: 1；Remaining is same as Example 1.
Table 1 lists the spore production being obtained using the method for embodiment and comparative example and spore rate：
Table 1
Table 1 as shown by data, embodiment 4 is that the injected volume of embodiment 1 is exaggerated 1000 times, but acquired results do not occur
Decay, illustrates this process stabilizing, possesses volume production basis.Embodiment 5 and embodiment 6 illustrate that the addition of zinc ion is conducive to spore
Rate lifting to a certain extent.
Table 2 is the investigation to this process repeatability, lists embodiment 1, embodiment 5 and embodiment 6 and is repeated 10 times respectively
The floating situation of obtained spore rate：
Table 2
| Embodiment 1 | Embodiment 5 | Embodiment 6 | |
| 1st time | 91% | 96% | 98% |
| 2nd time | 92% | 97% | 98% |
| 3rd time | 95% | 98% | 97% |
| The 4th | 93% | 97% | 98% |
| The 5th | 91% | 98% | 99% |
| 6th time | 96% | 96% | 98% |
| 7th time | 94% | 98% | 98% |
| 8th time | 93% | 96% | 99% |
| 9th time | 94% | 96% | 98% |
| 10th time | 91% | 98% | 97% |
| Average spore rate | 93% | 97% | 98% |
Table 2 shows that zinc ion and iron ion can reduce the fluctuating margin of spore rate, improves the repeatability of this technique, is beneficial to
Ensure product quality in large-scale production.And zinc ion and iron ion be applied in combination compared to independent that to add zinc ion permissible
Obtain higher data reappearance.Furthermore, it is necessary to illustrate, the impact to spore production of zinc ion and iron ion is little；And
The repeatability of the spore production in embodiment 1-4 is inherently very stable, need not be improved further again.
Although embodiment of the present invention is disclosed as above, it is not restricted to listed in description and embodiment
With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily
Realize other modification, therefore under the general concept being limited without departing substantially from claim and equivalency range, the present invention does not limit
In specific details.
Claims (7)
1. a kind of preparation method of bacillus spore is it is characterised in that comprise the following steps：
Step 1) trophocyte propagation：
The bacstearothermophilus of 1 weight portion are inoculated in the eutrophy culture medium of 10 weight portions, in 50-55 DEG C of environment,
After rotating speed is for culture 12h under the conditions of 150-250rpm/min；It is subsequently added the eutrophy culture medium of 90 weight portions, continue culture
12h, obtains trophocyte culture fluid；
Step 2) spore conversion：
Add the inorganic ionss culture medium of 1 weight portion in the described trophocyte culture fluid of 1 weight portion, in 55-60 DEG C of environment
In, rotating speed is culture 12h under the conditions of 100-150rpm/min；The inorganic ionss culture medium of 1 weight portion is subsequently added every 12h,
Until the gross mass part of inorganic ionss culture medium adding and the mass parts ratio of eutrophy culture medium reach 7: 1-10: 1；Obtain
Culture fluid containing bacillus spore.
2. the preparation method of bacillus spore according to claim 1 is it is characterised in that described eutrophy culture medium bag
Include：The soy peptone of 5-20 weight portion, the beef extract of 5 weight portions, 3-5 weight portion yeast extract, the chlorination of 5 weight portions
Sodium and the distilled water of 1000 weight portions.
3. the preparation method of bacillus spore according to claim 1 is it is characterised in that described eutrophy culture medium
PH is 7.2-7.5.
4. the preparation method of bacillus spore according to claim 1 is it is characterised in that described inorganic ionss culture medium
Including：The MnSO of NaCl, 0.5-1 weight portion of 1 weight portion4·H2The MgSO of O, 0.1-0.5 weight portion4·2H2O, 0.01-1 weight
The CaCl of amount part2·2H2The KH of O, 0.01-0.1 weight portion2PO4Distilled water with 1000 weight portions.
5. the preparation method of bacillus spore according to claim 1 is it is characterised in that described inorganic ionss culture medium
PH be 7.2-7.5.
6. the preparation method of bacillus spore according to claim 4 is it is characterised in that described inorganic ionss culture medium
Also include the ZnCl of 0.1-0.2 weight portion2.
7. the preparation method of bacillus spore according to claim 6 is it is characterised in that described inorganic ionss culture medium
Also include the FeCl of 0.1-0.2 weight portion3.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107475172A (en) * | 2017-08-30 | 2017-12-15 | 武汉工控工业技术研究院有限公司 | A kind of method for obtaining Geobacillus stearothermophilus gemma |
| CN108865937A (en) * | 2018-07-11 | 2018-11-23 | 青岛高迈生物科技有限公司 | It is a kind of to utilize the raw sporiferous method of bacillus stearothermophilus mutant bacteria |
| CN110438060A (en) * | 2019-07-22 | 2019-11-12 | 南京巨鲨显示科技有限公司 | A kind of preparation of Geobacillus stearothermophilus gemma, enrichment method |
| CN114410540A (en) * | 2022-02-07 | 2022-04-29 | 山东新华医疗器械股份有限公司 | Bacillus stearothermophilus culture solution capable of improving spore rate and culture method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0998938A2 (en) * | 1998-09-28 | 2000-05-10 | Herzog Mesmer, Artur, Dr. Dr. | Antimicrobials, methods of their manufacture and use thereof |
| US20050186661A1 (en) * | 2004-02-20 | 2005-08-25 | Rahman Raja Noor Zaliha A. | Production of protease from Bacillus stearothermophilus F1 |
| CN104593315A (en) * | 2015-01-06 | 2015-05-06 | 中国科学院苏州生物医学工程技术研究所 | Method for preparing spores of bacillus stearothermophilus |
| CN105018409A (en) * | 2014-04-22 | 2015-11-04 | 中国科学院沈阳应用生态研究所 | Methyl nutritional type bacillus spore-production fermentation medium and culture method thereof |
| CN105368746A (en) * | 2015-12-01 | 2016-03-02 | 山东新华医疗器械股份有限公司 | Preparation method of Bacillus stearothermophilus spores |
-
2016
- 2016-09-28 CN CN201610856595.XA patent/CN106434520B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0998938A2 (en) * | 1998-09-28 | 2000-05-10 | Herzog Mesmer, Artur, Dr. Dr. | Antimicrobials, methods of their manufacture and use thereof |
| US20050186661A1 (en) * | 2004-02-20 | 2005-08-25 | Rahman Raja Noor Zaliha A. | Production of protease from Bacillus stearothermophilus F1 |
| CN105018409A (en) * | 2014-04-22 | 2015-11-04 | 中国科学院沈阳应用生态研究所 | Methyl nutritional type bacillus spore-production fermentation medium and culture method thereof |
| CN104593315A (en) * | 2015-01-06 | 2015-05-06 | 中国科学院苏州生物医学工程技术研究所 | Method for preparing spores of bacillus stearothermophilus |
| CN105368746A (en) * | 2015-12-01 | 2016-03-02 | 山东新华医疗器械股份有限公司 | Preparation method of Bacillus stearothermophilus spores |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107475172A (en) * | 2017-08-30 | 2017-12-15 | 武汉工控工业技术研究院有限公司 | A kind of method for obtaining Geobacillus stearothermophilus gemma |
| CN108865937A (en) * | 2018-07-11 | 2018-11-23 | 青岛高迈生物科技有限公司 | It is a kind of to utilize the raw sporiferous method of bacillus stearothermophilus mutant bacteria |
| CN108865937B (en) * | 2018-07-11 | 2021-05-25 | 青岛创通生物科技有限公司 | Method for producing spores by using Bacillus stearothermophilus mutant |
| CN110438060A (en) * | 2019-07-22 | 2019-11-12 | 南京巨鲨显示科技有限公司 | A kind of preparation of Geobacillus stearothermophilus gemma, enrichment method |
| CN114410540A (en) * | 2022-02-07 | 2022-04-29 | 山东新华医疗器械股份有限公司 | Bacillus stearothermophilus culture solution capable of improving spore rate and culture method |
| CN114410540B (en) * | 2022-02-07 | 2024-01-19 | 山东新华医疗器械股份有限公司 | Bacillus stearothermophilus culture solution for improving spore rate and culture method |
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