CN106434379A - Burdock peel solid-state fermentation medium and method for fermenting flammulina velutipes - Google Patents
Burdock peel solid-state fermentation medium and method for fermenting flammulina velutipes Download PDFInfo
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- CN106434379A CN106434379A CN201610890933.1A CN201610890933A CN106434379A CN 106434379 A CN106434379 A CN 106434379A CN 201610890933 A CN201610890933 A CN 201610890933A CN 106434379 A CN106434379 A CN 106434379A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention discloses a burdock peel solid-state fermentation medium and a method for fermenting flammulina velutipes. The medium is prepared from burdock peel and water at a weight ratio of 5 to (1-2). The method comprises the following steps: inoculating a slant medium with a flammulina velutipes strain and conducting activating for 1-2 times; cleaning and peeling off burdock, cutting burdock peel to the size of (1-2)cm*(0.5-1)cm, naturally drying the burdock peel, mixing the dried burdock peel with water at a weight ratio of 5 to (1-2) and sterilizing an obtained mixture; cutting a strain block from the inactivated flammulina velutipes strain and inoculating the prepared solid-state fermentation medium with the strain block, and conducting fermentation cultivation so as to obtain the finished product (the burdock peel solid-state fermentation medium). According to the burdock peel solid-state fermentation medium and the method for fermenting flammulina velutipes provided by the invention, the burdock peel, which undergoes solid fermentation by virtue of the edible fungus, namely the flammulina velutipes, is decomposed and utilized and is converted into such bioactive substances as polysaccharide and the like, so that the problem that the burdock peel is low in utilization rate is solved, and the utilization use of burdock wastes is achieved and subsequently solid waste pollution is relieved.
Description
Technical field
The invention belongs to microbial solid fermentation technical field, specifically, it is related to a kind of Fructus Arctii skin solid fermentation culture
Base and the method for fermentation Flammulina velutiper (Fr.) Sing.
Background technology
Fructus Arctii is with the edible vegetable of fleshy tap root, Radix Arctii contain inulin, polysaccharide, saponin, flavonoid glycoside, dietary fiber,
The nutritional labeling that Polyphenols (as caffeic acid, chlorogenic acid, isochlorogenic acid etc.) and aldehyde material etc. enrich.Radix Arctii is new
The aspects such as type, functional type deep processed product, pharmacy, health product define more achievement in research.
While studying Radix Arctii energetically, but by as offal treatment, utilization rate is very low for substantial amounts of Fructus Arctii skin every year,
Cause greatly to waste, directly influence the economic benefit of peasant and processing enterprise.Research shows, total lignans in Fructus Arctii skin,
Content of cellulose is higher, contains a small amount of chlorogenic acid, polysaccharide simultaneously.If turning waste into wealth, except increasing farmers' income, also can subtract
The environmental pollution of few garbage.
Fructus Arctii garbage Fructus Arctii skin yield is big at present, not yet forms effective utilization ways, and main cause is to make
Contain substantial amounts of cellulose for Fructus Arctii skin, using difficulty.If biotechnology can be utilized, efficiently sharp using modern biotechnology
Use Fructus Arctii skin, solve the low difficult problem of Fructus Arctii resource utilization, to optimizing the Fructus Arctii utilization of resources and promote the sustainable of Fructus Arctii industry
Development has important practical significance.Still lack the research report of comprehensive utilization Fructus Arctii skin at present.
Content of the invention
In view of this, the present invention is directed to the low problem of above-mentioned Fructus Arctii skin utilization rate, there is provided a kind of Fructus Arctii skin solid fermentation
Culture medium and the method for fermentation Flammulina velutiper (Fr.) Sing, thus by Fructus Arctii garbage effectively utilizes.
In order to solve above-mentioned technical problem, the invention discloses a kind of Fructus Arctii skin solid fermentation culture medium, by Fructus Arctii skin and
Water by weight 5:1-2 makes.
The invention also discloses a kind of method of Fructus Arctii skin solid fermentation Flammulina velutiper (Fr.) Sing, comprise the following steps：
Actication of culture：Take Strains of Flammulina velutipes to be inoculated in activate 1-2 time on slant medium, obtain the golden mushroom activating
Kind；
Prepare solid fermentation culture medium：Fructus Arctii is cleaned, peeling, Fructus Arctii skin is cut into 1-2cm × 0.5-1cm size, natural
It is dried, dry Fructus Arctii skin and water are compared 5 according to weight:1-2 mixes, sterilizing；
Solid fermentation is cultivated：By the Needle mushroom strain having activated, clip one truffle is inoculated in the solid fermentation training preparing
In foster base, after fermentation culture, obtain product.
Further, described slant medium is potato dextrose agar slant culture medium.
Further, the temperature of described activation is specially 24-28 DEG C.
Further, described sterilizing is specially：Pressure 103.4KPa, 121 DEG C of high pressure steam sterilization 20min.
Further, specially 24-28 DEG C of the temperature of described fermentation culture, time are 10-15 days.
Compared with prior art, the present invention can obtain including following technique effect：
(1) method of the Fructus Arctii skin solid fermentation Flammulina velutiper (Fr.) Sing that the present invention provides, Fructus Arctii skin and water are compared 5 according to weight:1-2
Be formulated as solid fermentation culture medium, Needle mushroom strain carried out with fermentation culture, Fructus Arctii skin after edible fungi Flammulina velutiper (Fr.) Sing solid fermentation,
Be decomposed utilization, is converted into the bioactive substances such as polysaccharide, solves the problems, such as that Fructus Arctii skin utilization rate is low, thus Fructus Arctii is discarded
Thing effectively utilizes, and then decrease noxious waste pollution.
(2) method of the Fructus Arctii skin solid fermentation Flammulina velutiper (Fr.) Sing that the present invention provides, Fructus Arctii garbage is converted into available
High-value product, is conducive to the comprehensive utilization of Fructus Arctii.
(3) the Fructus Arctii skin solid fermentation culture medium that the present invention provides, composition is natural, without chemical additive, preparation method
Simplicity, with its Needle mushroom strain of fermenting, obtains the product time short.
Certainly, the arbitrary product implementing the present invention it is not absolutely required to reach all the above technique effect simultaneously.
Brief description
Accompanying drawing described herein is used for providing a further understanding of the present invention, constitutes the part of the present invention, this
Bright schematic description and description is used for explaining the present invention, does not constitute inappropriate limitation of the present invention.In the accompanying drawings：
Fig. 1 is that the embodiment of the present invention 1 solid fermentation cultivates 10 days wild Oryza species photos；
Fig. 2 is that the embodiment of the present invention 7 solid fermentation cultivates 15 days wild Oryza species photos；
Fig. 3 is the Flammukinan content that the embodiment of the present invention different material water weight ratio bottom fermentation obtains.
Specific embodiment
To describe embodiments of the present invention below in conjunction with embodiment in detail, thereby to the present invention how application technology handss
Section is solving technical problem and to reach realizing process and fully understanding and implement according to this of technology effect.
The present invention provides a kind of Fructus Arctii skin solid fermentation culture medium, by Fructus Arctii skin and water by weight 5:1-2 makes.
The present invention also provides a kind of method that Fructus Arctii skin ferments Flammulina velutiper (Fr.) Sing, comprises the following steps：
Step 1, actication of culture：Take Strains of Flammulina velutipes to be inoculated in activate 1-2 time on slant medium, in 24-28 DEG C of condition
Lower activation culture 1-2 time, obtains the Needle mushroom strain activating；
Step 2, prepares solid fermentation culture medium：Fructus Arctii is cleaned, peeling, it is big that Fructus Arctii skin is cut into 1-2cm × 0.5-1cm
Little, spontaneously dry, dry Fructus Arctii skin and water are compared 5 according to weight:1-2 mixes, and pH is natural, and pressure 103.4KPa, 121 DEG C of high pressure steam
Vapour sterilizing 20min；
The weight ratio of Fructus Arctii skin and water need to choose suitable proportion, and 5:1-2 works as Fructus Arctii skin and the weight ratio of water is less than
5:When 1, the Strains of Flammulina velutipes speed of growth is slow, and incubation time need to be more than 20 days, and mycelia just can cover with culture medium, is unfavorable for saving
Incubation time；When the weight of Fructus Arctii skin and water is than more than 5:When 2, because moisture is higher, Strains of Flammulina velutipes almost cannot be given birth to
Long.
Step 3, solid fermentation is cultivated：By the Needle mushroom strain having activated, clip one truffle is inoculated in the solid preparing
In fermentation medium, under the conditions of 24-28 DEG C, fermentation culture 10-15 days obtains product.
Preferably, described slant medium is potato dextrose agar slant culture medium.Specifically, potato glucose
Agar slant culture-medium, according to GB4789.15 2010《Microbiological test of food hygiene mycete and yeast counts》Prepare, use
Recovery and preservation in strain.
Needle mushroom strain of the present invention is edible fungi commonly used in the art, such as can be available from China General Microbiological
The CGMCC No.5.612 of culture presevation administrative center (CGMCC).
In the embodiment of the present invention, the mensure of polysaccharide adopts phend-sulphuric acid to measure, and is the normal of those skilled in the art's employing
Rule experimental technique, in following examples, crude polysaccharides content is the amount of contained polysaccharide in every gram of dried culture.
Embodiment 1
Step 1, actication of culture：Needle mushroom strain is inoculated on slant medium, such as potato dextrose agar slant
Culture medium, cultivates 7 days at 25 DEG C, covers with inclined-plane to mycelia；
Step 2, prepares solid fermentation culture medium：Fructus Arctii is cleaned, peeling, and Fructus Arctii skin is cut into 1.5cm × 0.5cm size, Gu
Body fermentation medium consists of dry Fructus Arctii skin and water, expects water weight ratio 5:1, pH is natural, pressure 103.4KPa, and 121 DEG C of high pressure steam
Vapour sterilizing 20min；
Step 3, solid fermentation is cultivated：The slant strains having activated are taken to be inoculated into equipped with the culture of 100g (dry weight) solid fermentation
In the 500mL culture bottle of base, 24 DEG C of fermentation culture 10 days, mycelia has covered with culture medium, referring specifically to Fig. 1.
The extraction of crude polysaccharides：Using decoction and alcohol sedimentation technique, it is method commonly used in the art.After solid fermentation terminates, take culture
It is placed in 40 DEG C of constant incubators to dry to constant weight, after pulverizing, weighs 1.0g, add 20 times of volume distilled water, 80 DEG C of extraction 4h.Cross
After filter, filtrate is concentrated into the 1/5 of former filtrate volume in 50 DEG C.After adding 5 times of volume dehydrated alcohol to mix, 4 DEG C of precipitate with ethanol are overnight.
4000r/min is centrifuged 5min, abandons supernatant, precipitation 75% ethanol purge 2-3 time, and 50 DEG C of drying precipitate, and obtain crude polysaccharides product.
The mensure of crude polysaccharides content：Using phend-sulphuric acid, it is method commonly used in the art.Crude polysaccharides 10mL distilled water
Dissolving 4h, draws 1mL crude polysaccharides solution, sequentially adds 6% phenol solution 1mL, concentrated sulphuric acid 5mL, mixes, room temperature stands 5min,
Boiling water bath heating 15min, mensuration absorbance value at 490nm, calculate crude polysaccharides content again.Recording crude polysaccharides content is
17.53mg/g.
Embodiment 2
Difference from Example 1 is, Fructus Arctii skin is cut into 1cm × 0.5cm size, records crude polysaccharides and contain after solid fermentation
Measure as 15.25mg/g.
Embodiment 3
Difference from Example 1 is, Fructus Arctii skin is cut into 2cm × 1cm size, records crude polysaccharides content after solid fermentation
For 13.68mg/g.
Embodiment 4
Difference from Example 1 is, solid fermentation culture base-material water weight ratio 5:Cultivate 10 days for 1.2,26 DEG C, solid
Recording crude polysaccharides content after fermentation is 14.55mg/g.
Embodiment 5
Difference from Example 1 is, solid fermentation culture base-material water weight ratio 5:Cultivate 11 days for 1.5,28 DEG C, solid
Recording crude polysaccharides content after fermentation is 15.21mg/g.
Embodiment 6
Difference from Example 1 is, solid fermentation culture base-material water weight ratio 5:Cultivate 12 days for 1.8,24 DEG C, solid
Recording crude polysaccharides content after fermentation is 10.20mg/g.
Embodiment 7
Difference from Example 1 is, solid fermentation culture base-material water weight ratio 5:Cultivate 15 days for 2,26 DEG C, mycelia length
Full culture medium (referring to Fig. 2), recording crude polysaccharides content after solid fermentation is 11.35mg/g.
Embodiment 8
Difference from Example 1 is, solid fermentation culture base-material water weight ratio 5:Cultivate 13 days for 2,28 DEG C, solid is sent out
Recording crude polysaccharides content after ferment is 10.27mg/g.
In the Flammulina velutiper (Fr.) Sing that different material water weight ratio bottom fermentations obtain, polyoses content is shown in Fig. 3, using method of the present invention fermentation
Flammulina velutiper (Fr.) Sing in crude polysaccharides content in more than 10.27mg/g, and work as solid fermentation culture base-material water weight ratio 5:When 1, Flammulina velutiper (Fr.) Sing
Middle crude polysaccharides content highest, is 17.53mg/g.
Fructus Arctii skin solid fermentation culture medium and the method for fermentation Flammulina velutiper (Fr.) Sing that the present invention provides, by Fructus Arctii skin and water according to weight
Amount compares 5:1-2 is formulated as solid fermentation culture medium, and Needle mushroom strain is carried out with fermentation culture, and Fructus Arctii skin is solid through edible fungi Flammulina velutiper (Fr.) Sing
After body fermentation, be decomposed utilization, is converted into the bioactive substances such as polysaccharide, solves the problems, such as that Fructus Arctii skin utilization rate is low, by cattle
Burdock garbage is converted into available high-value product, is conducive to the comprehensive utilization of Fructus Arctii, and then it is dirty to decrease solid waste
Dye.
Described above illustrate and describes some preferred embodiments of invention, but as previously mentioned it should be understood that inventing not
It is confined to form disclosed herein, be not to be taken as the exclusion to other embodiment, and can be used for various other combinations, modification
And environment, and can be carried out by the technology or knowledge of above-mentioned teaching or association area in invention contemplated scope described herein
Change.And the change that those skilled in the art are carried out and change without departing from the spirit and scope of invention, then all should weighed appended by invention
In the protection domain that profit requires.
Claims (6)
1. a kind of Fructus Arctii skin solid fermentation culture medium is it is characterised in that by Fructus Arctii skin and water by weight 5:1-2 makes.
2. a kind of method of Fructus Arctii skin solid fermentation Flammulina velutiper (Fr.) Sing is it is characterised in that comprise the following steps：
Actication of culture：Take Strains of Flammulina velutipes to be inoculated in activate 1-2 time on slant medium, obtain the Needle mushroom strain activating；
Prepare solid fermentation culture medium：Fructus Arctii is cleaned, peeling, Fructus Arctii skin is cut into 1-2cm × 0.5-1cm size, spontaneously dries,
Dry Fructus Arctii skin and water are compared 5 according to weight:1-2 mixes, sterilizing；
Solid fermentation is cultivated：By the Needle mushroom strain having activated, clip one truffle is inoculated in the solid fermentation culture medium preparing
In, obtain product after fermentation culture.
3. the method for Fructus Arctii skin solid fermentation Flammulina velutiper (Fr.) Sing as claimed in claim 2 is it is characterised in that described slant medium is
Potato dextrose agar slant culture medium.
4. the method for Fructus Arctii skin solid fermentation Flammulina velutiper (Fr.) Sing as claimed in claim 2 is it is characterised in that the temperature of described activation has
Body is 24-28 DEG C.
5. the method for Fructus Arctii skin solid fermentation Flammulina velutiper (Fr.) Sing as claimed in claim 2 is it is characterised in that described sterilizing is specially：
Pressure 103.4KPa, 121 DEG C of high pressure steam sterilization 20min.
6. the method for Fructus Arctii skin solid fermentation Flammulina velutiper (Fr.) Sing as claimed in claim 2 is it is characterised in that the temperature of described fermentation culture
Specially 24-28 DEG C of degree, time are 10-15 days.
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| CN201610890933.1A CN106434379A (en) | 2016-10-12 | 2016-10-12 | Burdock peel solid-state fermentation medium and method for fermenting flammulina velutipes |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107012101A (en) * | 2017-04-18 | 2017-08-04 | 徐州工程学院 | A kind of method for removing the peel burdock liquid fermentation medium and the new mushroom of fermented tea |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831472A (en) * | 2010-06-09 | 2010-09-15 | 福建省农业科学院农业工程技术研究所 | Method for preparing edible fungi soluble polysaccharide through solid fermentation by taking bean dregs as raw materials |
| CN102498935A (en) * | 2011-10-14 | 2012-06-20 | 福建农林大学 | Method for preparing flammulina velutipes nutrient solution by utilizing remains of food processing |
-
2016
- 2016-10-12 CN CN201610890933.1A patent/CN106434379A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831472A (en) * | 2010-06-09 | 2010-09-15 | 福建省农业科学院农业工程技术研究所 | Method for preparing edible fungi soluble polysaccharide through solid fermentation by taking bean dregs as raw materials |
| CN102498935A (en) * | 2011-10-14 | 2012-06-20 | 福建农林大学 | Method for preparing flammulina velutipes nutrient solution by utilizing remains of food processing |
Non-Patent Citations (1)
| Title |
|---|
| 苗敬芝等: "牛蒡中可溶性膳食纤维的提取", 《徐州工程学院学报（自然科学版）》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107012101A (en) * | 2017-04-18 | 2017-08-04 | 徐州工程学院 | A kind of method for removing the peel burdock liquid fermentation medium and the new mushroom of fermented tea |
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