CN106432528B - A kind of technique of the separation and Extraction Method of Polysaccharide in Radix Pinellia from the tuber of pinellia - Google Patents
A kind of technique of the separation and Extraction Method of Polysaccharide in Radix Pinellia from the tuber of pinellia Download PDFInfo
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- CN106432528B CN106432528B CN201610995100.1A CN201610995100A CN106432528B CN 106432528 B CN106432528 B CN 106432528B CN 201610995100 A CN201610995100 A CN 201610995100A CN 106432528 B CN106432528 B CN 106432528B
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The invention provides a kind of technique of the separation and Extraction Method of Polysaccharide in Radix Pinellia from the tuber of pinellia, the technique comprises the following steps：(1), the tuber of pinellia is dried or dried, is then crushed, sieving is made powder；(2), by powder and normal-temperature water according to 1:10~50 mass ratio mixing, pH is to faintly acid for regulation, stirs, then stands, takes upper liquid and filter, obtain filtrate；(3), by filtrate sufficient standing until wherein sediment is not further added by, isolate the sediment in filtrate, flushes sediment is cleaned, dry after i.e. obtained Method of Polysaccharide in Radix Pinellia.Present invention process is simple, it is only necessary to which carrying out several normal process steps can complete to extract.Need not be heated in the present invention, ultrafiltration, the step such as alcohol extracting, reduce the cost of extraction, serve the effect of energy-conserving and environment-protective.It is breakthrough in the present invention to employ low temperature water extraction process, therefore the Method of Polysaccharide in Radix Pinellia yield extracted is high, purity is good.
Description
Technical field
The invention provides a kind of separating and extracting process of polysaccharide, more particularly to a kind of separation and Extraction polysaccharide from the tuber of pinellia
Method.
Background technology
The tuber of pinellia (scientific name：Pinellia ternata), also known as ground text, keep field etc., belong to Arales.It is distributed widely in China
The area such as the Yangtze river basin and northeast, North China.It is medicinal plant, has eliminating dampness and eliminating phlegm, stopping nausea and vomiting by lowering the adverse flow of QI, and the raw furuncle that disappears is made
With, animal doctor controlling synanche Huang.It is also distributed in Tibet, is grown in height above sea level 3000m or so area.
Method of Polysaccharide in Radix Pinellia is the function ingredients in the tuber of pinellia, at present in the extraction process for Method of Polysaccharide in Radix Pinellia, mainly water extraction
Alcohol deposition method, the method in ultrafiltration and the patent document of Patent No. 2011100493541.1.Above method has one altogether
Same part, all it is that heating decocts when being exactly water extraction, then with ethanol precipitation, it is also necessary in prolonged vacuum concentration and bag filter
Dialyse, totally ten one procedure.
Heating decocts when water extraction is used in process above, it may appear that following two problems, when because temperature is too high, the time
It is long, polysaccharide meeting water swelling, gelatinization, come for subsequent extracted process bands difficult；Second, hot water can dissolve account for whole polysaccharide 10~
20% amylose, and be difficult to dissolve the amylopectin for accounting for 80~90%, so easily being lost with hot water boiling and extraction polysaccharide
Substantial portion of polysaccharide.In addition, in the patent document of the patent No. 2011100493541.1, add 95% ethanol and adjust to containing alcohol
Amount filters after standing 24h up to 60%, takes supernatant → vacuum concentration → dialysis → merging concentrate → drying purifying → tuber of pinellia more
Sugar.The method of this extraction polysaccharide, yield will not be highly desirable, because polysaccharide does not dissolve in ethanol, in the supernatant of ethanol
The content very little of polysaccharide, it is difficult to obtain polysaccharide to be concentrated.
In summary, extracting method technological process used in the art is more complicated, and required time is long,
Extraction cost is higher, and polysaccharide yield is undesirable.
The content of the invention
The present invention solves deficiency of the prior art, and a kind of breakthrough directly use that propose is extracted under cold water normal temperature
The technique of Method of Polysaccharide in Radix Pinellia, the technological process is simple, easily operated, and not only production cost is low, and yield is high, good product quality.
Technical scheme is used by realizing above-mentioned purpose of the present invention：
A kind of technique of the separation and Extraction Method of Polysaccharide in Radix Pinellia from the tuber of pinellia, the technique comprise the following steps：(1), the tuber of pinellia is dried
Or drying, then crush, sieving is made powder；(2), by powder and normal-temperature water according to 1:10~50 mass ratio mixing, regulation
PH is stirred, then stood, take upper liquid and filter, obtain filtrate to faintly acid；(3) it is, that filtrate sufficient standing is straight
Be not further added by wherein sediment, isolate the sediment in filtrate, flushes sediment is cleaned, dry after i.e. be made the tuber of pinellia it is more
Sugar.
Preferably, 60 mesh sieves are crossed after raw material is crushed in step (1) is either more than 60 mesh sieves.
Preferably, the temperature of normal-temperature water described in step (2) is no more than 30 DEG C.
Preferably, mixing time is 3~5 minutes in step (2), time of repose is 5~10 minutes.
Preferably, filtered in step (2) using the byssus of individual layer.
Preferably, time of repose is 1~4 hour in step (3).
Remaining sediment merges with remaining filter residue after filtering after being stood in step (2) and taking upper liquid away,
Repeat the extraction process in step (2) and step (3) as raw material, repeat extraction 2~3 times, and the tuber of pinellia that will be extracted
Polysaccharide merges.
In extraction process provided by the present invention, because inventor has recognized extraction of the high temperature for Method of Polysaccharide in Radix Pinellia not
Profit influences, the creative idea for proposing normal-temperature water (cold water) extraction, is no more than 30 DEG C of water using liquid temperature in this application
Extracted, because polysaccharide does not dissolve in cold water, and when liquid temperature is up to or over 50 DEG C, the time one is grown, and polysaccharide will be inhaled
Water expands, gelatinization, so as to influence precipitation separation.In addition, first by raw mill into powder in this technique, and the particle diameter of powder
The smaller the better, this is due to that raw material crushes thinner, and polysaccharide therein is easier to be taken out by water extraction.At the same time, this is special
Solid-liquid ratio employed in profit is different from solid-liquid ratio of the prior art, due to inventors realized that in feed liquid mixing liquid,
The concentration of material is more low better, and concentration is lower, and polysaccharide therein more easily precipitates, thus in the art by powder with
Normal-temperature water is according to 1:10~50 mass ratio is mixed.PH is adjusted to faintly acid in extraction in this technique, and purpose is to protect
The stability of tuber of pinellia solution colloid is held, in favor of the precipitation of polysaccharide, improves the purity of polysaccharide.Last standing 1~4 is small in this technique
When, the time is long, it is therefore an objective to ensures that polysaccharide has enough time precipitation complete, improves the yield of polysaccharide as far as possible.
In summary, technical scheme provided by the present invention has advantages below compared with prior art：1st, work of the present invention
Skill is simple, it is only necessary to which carrying out several normal process steps can complete.2nd, need not be heated in the present invention, ultrafiltration, the step such as alcohol extracting
Suddenly, the cost of extraction is reduced, serves the effect of energy-conserving and environment-protective.3rd, the water at low temperature that employs breakthrough in the present invention extracts work
Skill, therefore the Method of Polysaccharide in Radix Pinellia yield extracted is high, purity is good.
Embodiment
Detailed specific description is done to the present invention with reference to specific embodiment, but protection scope of the present invention not office
It is limited to following examples.
Embodiment
Concrete technology is as follows in the present embodiment：By the raw material tuber of pinellia, crushed after drying, cross 80 mesh sieves, claim 10 grams of dry powder, added
In the cold water that 20 DEG C of 150ml water temperatures, regulation pH value is 4.2, is stirred 3 minutes, stands 10 minutes, takes out upper liquid, filtering, will filter
Liquid stands 3 hours, isolates bottom sediment, as Method of Polysaccharide in Radix Pinellia crude product；Then by the filter residue filtered to isolate with standing 10
Minute retain sediment merge, add water 150ml stir 3 minutes, stand 10 minutes, start repeat extraction polysaccharide it is secondary, merge
The Method of Polysaccharide in Radix Pinellia crude product of separation and Extraction three times, is washed secondary with cold water, goes the removal of impurity, is dried, is produced Method of Polysaccharide in Radix Pinellia crude product 1.25
Gram.The yield of final product is 12.5%.
Claims (6)
1. a kind of technique of the separation and Extraction Method of Polysaccharide in Radix Pinellia from the tuber of pinellia, it is characterised in that the technique comprises the following steps：(1), will
The tuber of pinellia is dried or dried, and is then crushed, sieving is made powder；(2), by powder and normal-temperature water according to 1:10~50 mass ratio mixes
Close, pH is to faintly acid for regulation, stirs, then stands, takes upper liquid and filter, obtain filtrate；(3), filtrate is filled
Point stand until wherein sediment be not further added by, isolate the sediment in filtrate, flushes sediment is cleaned, dry after i.e. system
Obtain Method of Polysaccharide in Radix Pinellia.
2. the technique of the separation and Extraction Method of Polysaccharide in Radix Pinellia according to claim 1 from the tuber of pinellia, it is characterised in that：In step (1)
60 mesh sieves are crossed after raw material is crushed and are either more than 60 mesh sieves.
3. the technique of the separation and Extraction Method of Polysaccharide in Radix Pinellia according to claim 1 from the tuber of pinellia, it is characterised in that：In step (2)
The temperature of the normal-temperature water is no more than 30 DEG C.
4. the technique of the separation and Extraction Method of Polysaccharide in Radix Pinellia according to claim 1 from the tuber of pinellia, it is characterised in that：In step (2)
Mixing time is 3~5 minutes, and time of repose is 5~10 minutes.
5. the technique of the separation and Extraction Method of Polysaccharide in Radix Pinellia according to claim 1 from the tuber of pinellia, it is characterised in that：In step (3)
Time of repose is 1~4 hour.
6. the technique of the separation and Extraction Method of Polysaccharide in Radix Pinellia according to claim 1 from the tuber of pinellia, it is characterised in that：By step (2)
It is middle stand and take after upper liquid remaining sediment away and filter after remaining filter residue merge, repeat to walk as raw material
Suddenly the extraction process in (2) and step (3), extraction 2~3 times is repeated, and the Method of Polysaccharide in Radix Pinellia extracted is merged.
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| CN1036067C (en) * | 1994-08-24 | 1997-10-08 | 沈阳医药工业研究所 | Method for extracting protein and polysaccharide from spiral algae and its use |
| CN101264311B (en) * | 2008-04-21 | 2010-08-25 | 青岛大学 | Natural pharmaceutical composition, its preparation and its attenuation synergistic use in cancer radiotherapy and chemotherapy |
| CN102133386A (en) * | 2011-03-02 | 2011-07-27 | 青岛大学 | Tuber pinellia and dried ginger composition, preparation method thereof and application of tuber pinellia and dried ginger composition in preparation of attenuated synergistic medicaments in cancer radiotherapy and chemotherapy |
| CN105213523B (en) * | 2015-09-10 | 2019-03-15 | 杨丽敏 | A kind of purposes of method and the extract that extracting effective ingredient from the coptis |
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