CN106420852B - Atomized broadleaf holly leaf and application - Google Patents
Atomized broadleaf holly leaf and application Download PDFInfo
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Abstract
The invention provides atomized broadleaf holly leaf which is prepared from holly plants serving as raw materials by adopting three formulations of a spray, an aerosol and a powder spray, has the effects of improving mouthfeel, relieving cough and ventilating and health care, and can be used for health care of oral cavity and lung and adjuvant treatment of lung diseases. The invention discloses a preparation method and component composition of atomized broadleaf holly leaf. The invention can be widely used for the industrial production of atomized broadleaf holly leaf.
Description
Technical Field
The present invention relates to a functional health-care product and its preparation method
Background
The broadleaf holly leaf has a long application in the south of China and has a thousand-year history. It is described that various plants can be used in the manufacture of broadleaf holly leaf, including: trifoliaceae (Oleaceae) plants, Aquifoliaceae (Aquifoliaceae) plants, Guttiferae (Guttiferae) plants, Boraginaceae (Boraginaceae) plants, Verbenaceae (Verbenacae) plants, and the like. According to the records, the main plants comprise glossy privet fruits, holly fruits, Chinese holly fruits, broad-leaved Chinese mahonia and the like. The efficacy of broadleaf holly leaf is relatively complex, and mainly comprises: diminishing inflammation, detoxicating, sterilizing, relieving itching, clearing throat, relieving cough, etc. The pharmacological actions of the broadleaf holly leaf include: inhibiting thrombosis and platelet aggregation; regulating blood pressure; regulating blood lipid; weight reducing, blood sugar regulating, antioxidant, antiinflammatory, analgesic, immunity regulating, antibacterial, cough relieving, and phlegm eliminating effects. Clinically, the broadleaf holly leaf is taken orally to regulate blood pressure, regulate blood fat, prevent and treat phlebitis, care oral cavity, treat skin diseases and the like. At present, the taking mode of the broadleaf holly leaf mainly comprises the following steps: conventional water-soaked tea, tea bag, instant granule, buccal tablet, beer, capsule or tea beverage. The broadleaf holly leaf beverage is mainly a water extract of tea, is subjected to simple extraction and functional component enrichment (saponin is more than 20 percent, polyphenol is more than 16 percent, and flavone is more than 13 percent), and is applied to the beverage in an amount of 0.25 g/kg. The raw materials of the broadleaf holly leaf are complex in source and have unclear relation with the efficacy, so that the application of the broadleaf holly leaf is limited. The broadleaf holly leaf has extremely bitter and astringent taste and poor mouthfeel, and the popularization and application of the broadleaf holly leaf are influenced. How to improve the taste of the broadleaf holly leaf and to determine the efficacy of the broadleaf holly leaf is an important subject of the research on the broadleaf holly leaf. The invention describes a preparation method and application of atomized broadleaf holly leaf. The atomized broadleaf holly leaf tea has the advantages of faint scent, no astringent taste and convenient use, has definite efficacy on relieving cough, is very suitable for auxiliary treatment of oral cavity and lung diseases of families, carry-ons and hospitals, and can be industrially produced in batches.
Disclosure of Invention
1. The invention aims to provide atomized broadleaf holly leaf and a preparation method thereof.
2. It is a further object of the present invention to provide the use of the above atomized kudingcha.
Technical scheme
The purpose of the invention is realized by the following technical scheme, which is characterized in that:
selecting the raw materials of the ilex:the plant parts of Aquifoliaceae including leaves, roots, stems, bark, and fruits are selected. The ilex plants mainly comprise: ilex latifolia, ilex microphylla, ilex latifolia, ilex pubescens, ilex rotunda, ilex pubescens, ilex verticillata, ilex pubescens, ilex latifolia, and other ilex plants.
Atomized broadleaf holly leaf extraction method: the method is characterized by comprising the following steps:
weighing dried raw material plants: the leaf of ilex latifolia Thunb, ilex pubescens, ilex ferruginea L, etc. is preferably selected. In order to increase the dissolution rate, the dried leaves of the plant or the finished product of the broadleaf holly leaf can be firstly crushed and then soaked. Alcohol soaking: the alcohol soaking should be carried out for 4-72 hours or more. Ultrasonic treatment or circular distillation can be used as auxiliary agent to improve the dissolution efficiency. Other food grade or medicinal organic solvents can also be used for dissolving. The recommended concentration of alcohol is 40-100%.
Resin decoloring: in order to improve the taste, appearance and efficacy, further decolorization treatment is carried out, and macroporous resin is recommended. Preparing an aqueous solution: evaporating the liquid to dryness to obtain fixed powder, dissolving with water, filtering, adjusting concentration to 5-50 g/L, bottling, and packaging.
Component determination: the components of the broadleaf holly leaf are characterized by comprising, by weight, triterpenes and triterpenoid saponins (0.5-80%, preferably 0.5-70%), polyphenols (0.01-30%), flavonoids (0.01-30%) and soluble polysaccharides (0.01-15%, preferably 0.01-10%).
Atomized broadleaf holly leaf production method: the method is characterized by comprising the following manufacturing steps of at least three atomized dosage forms:
1. mixing the extractive folium Ilicis solution with adjuvants, filtering, and making into spray.
2. Adding propellant into the extractive folium Ilicis solution, and making into aerosol.
3. Making the extracted folium Ilicis into powder, adding adjuvant, and making into powder spray.
Administration mode of atomized broadleaf holly leaf: it is characterized in that the medicine is taken through mouth and nose by adopting a spraying mode.
Application of atomized broadleaf holly leaf：
The function of the broadleaf holly leaf is very complex, and according to the current clinical report, the broadleaf holly leaf is orally taken to regulate blood pressure, regulate blood fat, prevent and treat phlebitis, oral care, skin disease treatment and the like. Because the raw materials for making the broadleaf holly leaf are often different, the relationship between the raw materials and the efficacy is not clear. The atomized broadleaf holly leaf provided by the invention has the relationship between raw materials and effects. The efficacy of the broadleaf holly leaf described by the invention is characterized in that the broadleaf holly leaf prepared from ilex plants has obvious cough relieving efficacy, and the effect is more obvious by an administration mode of atomization and inhalation.
Advantageous effects
The invention only selects the ilex plants as raw materials and prepares the ilex tea which is taken in an atomized form, and the atomized ilex tea has very definite cough relieving and ventilation effects. The invention enhances the efficacy and improves the taste of the broadleaf holly leaf by taking an organic solvent as a basic extraction method and assisting in decoloring. The invention has the beneficial effects that the defects of poor taste and weak cough relieving effect of the broadleaf holly leaf extracted by common water are overcome, and a novel administration mode of the broadleaf holly leaf is invented, so that the broadleaf holly leaf tea has wide value in popularization and application.
Drawings
FIG. 1. efficiency of the preparation process of raw material of Folum Ilicis
FIG. 2 chemical composition (w/w) of raw material of Folum Ilicis
FIG. 3 evaluation results of the taste of Folum Ilicis
FIG. 4 influence of atomized ilex latifolia on cough frequency and cough latency in mice
FIG. 5 shows the cough relieving effect of Folum Ilicis from different plant sources
FIG. 6 comparison of cough relieving effect of three kinds of atomized Kuding tea
Detailed Description
EXAMPLE 1 preparation of atomized Kuding tea feedstock
At present, the preparation of the broadleaf holly leaf is mainly a water soaking method, and the broadleaf holly leaf is directly soaked in water for drinking, is soaked in water and then is dried by distillation (drying) to prepare powder for taking after being mixed with water, and the tea leaves are crushed and then taken after being mixed with water. In order to improve the taste and the efficacy, the invention adopts organic solvents with different concentrations for extraction, and because the most common edible organic solvent at present is alcohol, the alcohol extraction is taken as an example. On the basis, the appearance is improved and the efficacy is further improved through decoloring treatment.
Materials and methods
Raw materials of broadleaf holly leaf: ilex kudingcha ilex (Anhui Huilong) and ilex latifolia kudingcha (Anhui).
Chemical reagents: ethanol (chemical purity), n-butanol (chemical purity), macroporous resin
Preparing an alcohol extracting solution: weighing 1000 g of broadleaf holly leaf raw material, and crushing by a crusher. According to the following steps: 1-10, soaking for 72 hours in 70% ethanol, evaporating to dryness, and mixing with the mixture according to the weight ratio of 1: adding distilled water at a ratio of 50-200(w/v) to dissolve completely, and filtering.
Preparation of decolorized extract: mixing the alcohol extract in a ratio of 1: loading the mixture into macroporous resin at a ratio of 20-100(v/v), eluting with 20-60% alcohol for decolorizing, eluting with 60-100% alcohol, and evaporating the eluate to dryness in rotary evaporator to obtain white or yellowish solid tea.
Results and conclusions
By adopting the method, 200-300 g of ethanol extract can be obtained per kilogram of tea. On the basis, 20-30 g of decolored extract can be obtained by decoloration treatment. The extraction efficiency of ilex latifolia is basically equivalent to that of ilex latifolia, the total yield of the two ilex latifolia preparations is about 1-4%, and detailed data are shown in figure 1.
EXAMPLE 2 analysis of chemical composition of raw material of Folum Ilicis
Materials and methods
Raw materials of broadleaf holly leaf: comprises two raw materials of undeloured and decolored, which are provided by Nanjing Musai biotechnology company, and the plant raw material is ilex latifolia. The production process is the same as that of example 1. the concentrations of the two raw materials of broadleaf holly leaf are both 100 mg/ml.
The analysis method comprises the following steps: all refer to classical determination methods described in pharmacopoeia. And (3) quantitative determination of triterpenoid saponin: taking the broadleaf holly leaf saponin F as a standard substance, diluting a sample to be detected (3mg/mL) by 100 times, and then determining the content. And (3) total flavone quantification: and (3) diluting the sample to be detected by 50 times by using rutin as a standard substance, and then determining the content. And (3) quantifying polyphenol: and (4) measuring by a Folin-phenol method. And (3) soluble polysaccharide quantification: indirect method.
Results and conclusions
The undecolored folium Ilicis solution mainly contains triterpene, triterpenoid saponin, flavone, polyphenol and soluble polysaccharide, and the content is 10-20% by conventional quantitative method. The decolorized broadleaf holly leaf mainly contains triterpenoid saponins, the total content is 50-90%, and the contents of polyphenols, flavones, soluble polysaccharides and other substances are less than 2%. The detailed results are shown in FIG. 2.
EXAMPLE 3 mouthfeel improvement of atomized Kuding tea
The Folum Ilicis prepared by conventional soaking method contains abundant tannin, foreign protein, pigment, etc., has bitter taste and short aroma duration, and can precipitate and change color when the aqueous solution is left for a long time. To overcome the problem, the method of ethanol soaking is adopted for extraction, and the taste is measured. Dissolving the ethanol extract with distilled water, filtering, making into 5mg/mL ethanol-extracted Folum Ilicis (tea I), adding tween-80 and sodium chloride adjuvants, and making into spray. Preparing decolorized Folum Ilicis (tea II) with the same concentration (5mg/mL) by the same method, and making into spray. As a control, we prepared water-extracted broadleaf holly leaf. 100 g of ilex latifolia thumb leaf is taken, soaked in 1000 ml of hot water with the temperature of 90-100 ℃ for 30-60 minutes, and the tea water is evaporated to dryness and weighed. Preparing water-extracted Folum Ilicis (tea III) 5mg/mL with water, and making into spray. The method for testing the mouthfeel by adopting a double-blind method mainly comprises the following steps:
1. 20 tested volunteers aged 20-55 years, 10 male and 10 female were selected, all were healthy, had no metabolic disease and oral disease, and except 1 smoker, the rest were non-smokers.
2. The test method comprises the following steps: the test subjects rinsed their mouth with fresh water (farmer spring) once, received a mouth spray (approximately 100 microliters per solution) once within 1 minute, and filled out the questionnaire. The mouth is rinsed once more before receiving the second spray, and so on.
Taste test questionnaires/answers (examples)
Results
The 20 answer sheet statistics are shown in figure 3 below. The astringency score was highest for tea II and highest for tea III; tea II has similar aroma degree to tea I, but is significantly higher than tea III; the bitterness of tea II is slightly reduced; tea II has a better aftertaste than tea I and tea III.
Conclusion
The broadleaf holly leaf prepared by the alcohol extraction method can improve the fragrance degree and reduce the astringency, and after the decoloration treatment, the astringency is further reduced, the bitterness is also slightly reduced, but the aftertaste is obviously improved. The comprehensive taste of the broadleaf holly leaves processed by the alcohol extraction is obviously better than that of the broadleaf holly leaves processed by the water extraction.
EXAMPLE 4 evaluation of antitussive efficacy of atomized Kuding tea
Through continuous groping, the atomized broadleaf holly leaf prepared by the invention is found to have a good effect on cough caused by tracheitis of a human, and the tea is presumed to have a cough relieving effect. To study the efficacy of the system, we evaluated it in a mouse model.
Materials and methods
Reagent: concentrated ammonia (analytically pure) (25-28%) (Chengdu Kelong) was made into 13% solution before each experiment.
The method comprises the following steps: 50 ml of freshly prepared 13% ammonia were added to an ultrasonic nebulizer, mice (C57BL/6J) (purchased from the model animal research institute, university of Nanjing) were placed in a closed squirrel cage, the nebulization outlet was connected to the cage and aerated continuously for 60 seconds, then stopped and left closed. While ventilating, a stopwatch was used to record the number of coughs and cough latencies over 3 minutes. The drug treatment group is that 5mg/mL of atomized broadleaf holly leaf solution and 26% concentrated ammonia water are mixed in equal amount, and the cough frequency and the cough latency are calculated by the same method in an ultrasonic atomizer. The number of coughs per minute is less than 10 or more than 80, we consider abnormal variation and do not enter statistical data; mice with cough latencies less than 10 seconds or greater than 50 seconds were not included in the statistics.
Results
By taking water as a control, after the mice are stimulated by ammonia water, the cough frequency is 20-30 times within three minutes, the average is 25 +/-11 times, the cough latency is about 30 seconds, and the cough latency is equivalent to that reported in the past, so that the established cough determination model is feasible. The treatment with tea I, tea II and tea III respectively has significantly less cough frequency than control group (p <0.05 or p <0.01) in three minutes, and significantly prolonged cough latency (p < 0.05). Tea II can reduce the cough frequency of 3 minutes by more than 50% (11 + -4 vs 25 + -11) and has the effect obviously better than that of tea I or tea III, and the cough latency time is prolonged by about 17 seconds (see figure 4 for details).
EXAMPLE 5 comparison of the efficacy of different botanical sources of Folum Ilicis
The plant components of the broadleaf holly leaf are complex, and the efficacy is also various. To find the relationship between Kuding tea plants and cough relieving efficacy, we compared the cough relieving effects of different plants.
Method of producing a composite material
The extraction method of the plant is the same as that of tea I, namely soaking with alcohol, evaporating to dryness, and dissolving with water again. The cough-relieving effect of each extract was measured by ammonia water stimulation.
Results
By comparing the cough times in 3 minutes, we find that the ilex plants can obviously reduce the cough times, the inhibition efficiency is about 40-50%, and other plants have no obvious cough-relieving effect.
EXAMPLE 6 preparation and efficacy comparison of different formulations of atomized Folum Ilicis
The current common methods of aerosolization are primarily accomplished by three dosage forms, namely sprays, aerosols, and dusts. In order to compare the advantages and disadvantages of the three formulations, atomized broadleaf holly leaves (collectively called atomized broadleaf holly leaves) of the three formulations are prepared, and the advantages and disadvantages are evaluated through efficacy and effect. Since the undecolored broadleaf holly leaf raw material is in the form of an extract, it is difficult to simultaneously prepare three forms of atomized formulations, so that the decolorized broadleaf holly leaf (tea II) is used as the tea raw material to respectively prepare the three atomized formulations.
Preparation of broadleaf holly leaf spray
Taking decolorized Folum Ilicis (tea II) as tea raw material, preparing Folum Ilicis solution with different concentrations (0.5-50mg/mL) with distilled water, 2.8% Tween-80 (w/w), and sodium chloride (0.75mg/mL) as adjuvants, filtering with 0.22 μm microfilter, and bottling.
Preparation of aerosol broadleaf holly leaf tea
Preparing medicinal liquid according to aerosol formulation (different amount of Folum Ilicis, distilled water 5 g, and anhydrous alcohol 150 g), and spraying the medicinal liquid 0.05-5mg (equivalent to liquid concentration of 0.5-50mg/mL) once. Filling into container at room temperature, installing and fastening valve, and pressing into HFA-134a by press
Preparation of powder spray agent broadleaf holly leaf
Firstly, the broadleaf holly leaf is crushed, xylitol is added into the broadleaf holly leaf with the granularity less than 10 mu m as an additive, and then the broadleaf holly leaf is added into a reservoir type drug delivery device.
Experimental treatment method
50 ml of freshly prepared 13% ammonia were added to an ultrasonic nebulizer, mice (C57BL/6J) (purchased from the model animal research institute, university of Nanjing) were placed in a closed squirrel cage, the nebulization outlet was connected to the cage and aerated continuously for 60 seconds, then stopped and left closed. At the start of the nebulisation, the cage was sprayed with a continuous spray of tea solution for 60 seconds, with 2 second intervals, about 20 times in total. After the injection, the squirrel cage was closed, and the cough count was counted by another experimenter at the beginning of the injection using a stopwatch. The number of coughs in 3 minutes was counted with a stopwatch immediately after the start of atomization. After the injection, the squirrel cage was closed, and the cough count was counted by another experimenter at the beginning of the injection using a stopwatch. The method for determining efficacy of Folum Ilicis as propellant is the same as that of Folum Ilicis with sprayer.
Results
The cough model is manufactured by adopting an ultrasonic ammonia water atomization method, atomized water (the content of the broadleaf holly leaf is 0mg/ml) is used for simulation treatment, and the cough frequency of every 3 minutes is 25 +/-11. When the broadleaf holly leaf spray is used for treating the cough, the cough frequency is obviously reduced to 18 +/-8 (p is less than 0.05), when the dose reaches 5mg/mL, the cough frequency is reduced to 11 +/-5 (p is less than 0.05), and when the dose is continuously increased to 50mg/mL, the inhibition effect cannot be further improved (12 +/-7, p is less than 0.05). When the dose of the broadleaf holly leaf aerosol is 5mg/mL, a certain cough relieving effect (13 +/-7) begins to be displayed, and the cough relieving effect is slightly weaker than that of the broadleaf holly leaf aerosol, but no obvious difference exists. When the powder inhalation broadleaf holly leaf is 5mg/mL, the cough relieving efficiency reaches 50%, and the effect can be further improved by increasing the dosage (figure 6).
According to the results, the broadleaf holly leaf spray, the broadleaf holly leaf aerosol and the broadleaf holly leaf powder spray have obvious cough relieving effects. Although the effect of the aerosol broadleaf holly leaf and the powder aerosol broadleaf holly leaf is slightly lower than that of the aerosol, the reason that the atomization effect of the atomized broadleaf holly leaf is poor and the mouse inhalation amount is small can be probably also the reason of experimental error.
Claims (17)
1. An atomized broadleaf holly leaf, wherein the broadleaf holly leaf is administered by atomization, and the atomized broadleaf holly leaf comprises:
triterpene saponins: 50-90 percent of,
And (3) flavone: 0.01 to 2 percent of,
Polyphenols: 0.01 to 2 percent of,
Soluble polysaccharide: 0.01-2% by weight.
2. An atomised tea of claim 1 which is a spray, aerosol or powder.
3. The aerosolized Kuding tea of claim 1, wherein the aerosolized Kuding tea is prepared from a plant of the family Aquifoliaceae.
4. An atomised tea leaf of claim 3, wherein the atomised raw material is obtained from a plant of the family Aquifoliaceae by extraction with an organic solvent and decolourisation.
5. An atomised broadleaf holly leaf according to claim 4, wherein said organic solvent comprises: alcohol, n-butanol, methanol, diethyl ether, dichloromethane, propylene glycol, isopropanol, chloroform and acetone, wherein the concentration of the organic solvent is 0-100%.
6. An atomised broadleaf holly leaf according to claim 5, wherein said organic solvent is 40-100% alcohol.
7. Atomized broadleaf holly leaf according to claim 4, characterized in that the decolorization treatment uses macroporous resins for decolorization.
8. Atomized broadleaf holly leaf according to claim 7, wherein the resin is a non-or weakly polar resin.
9. The aerosolized broadleaf holly leaf of claim 3, wherein said Aquifoliaceae plant is selected from the group consisting of: ilex latifolia, ilex microphylla, ilex latifolia, ilex pubescens, ilex rotunda, ilex thunbergii, ilex siccus, ilex latifolia, ilex pubescens, ilex verticillata, ilex pubescens, and ilex macrocarpa.
10. A process for preparing an atomized ilex latifolia thumb as claimed in claim 1, characterized in that ilex latifolia thumb raw material is obtained by soaking, extracting and decolorizing ilex latifolia thumb raw material of Aquifoliaceae, and then made into aerosol, spray or powder.
11. The method of claim 10, wherein the extraction is with an organic solvent.
12. The method of claim 11, wherein the organic solvent comprises: alcohol, n-butanol, methanol, diethyl ether, dichloromethane, propylene glycol, isopropanol, chloroform and acetone, wherein the concentration of the organic solvent is 0-100%.
13. The method of claim 12, wherein the organic solvent is 40-100% alcohol.
14. The method of claim 10, wherein the decolorizing treatment employs macroporous resins for decolorizing.
15. The method of claim 14, wherein the resin is a non-or weakly polar resin.
16. The method of claim 10, wherein said Aquifoliaceae plant is selected from the group consisting of: ilex latifolia, ilex microphylla, ilex latifolia, ilex pubescens, ilex rotunda, ilex thunbergii, ilex siccus, ilex latifolia, ilex pubescens, ilex verticillata, ilex pubescens, and ilex macrocarpa.
17. Use of atomised broadleaf holly leaf as claimed in any one of claims 1 to 9 in the manufacture of a medicament for cough suppressing and ventilation.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101791324A (en) * | 2010-04-07 | 2010-08-04 | 苏州瑞得符生物科技有限公司 | Preparation process of Ilex latifolia Thunb extractive |
| CN102329365A (en) * | 2011-07-06 | 2012-01-25 | 南京泽朗农业发展有限公司 | Method for separating and preparing ilexin II |
| CN102600214A (en) * | 2011-01-24 | 2012-07-25 | 北京大学 | Method for extracting leaf of Chinese ilex of broadleaf holly leaf, total saponin and application thereof |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101791324A (en) * | 2010-04-07 | 2010-08-04 | 苏州瑞得符生物科技有限公司 | Preparation process of Ilex latifolia Thunb extractive |
| CN102600214A (en) * | 2011-01-24 | 2012-07-25 | 北京大学 | Method for extracting leaf of Chinese ilex of broadleaf holly leaf, total saponin and application thereof |
| CN102329365A (en) * | 2011-07-06 | 2012-01-25 | 南京泽朗农业发展有限公司 | Method for separating and preparing ilexin II |
Non-Patent Citations (2)
| Title |
|---|
| 毛冬青咽喉炎喷雾剂研究;陈根凯,陈辉芳,杨凤琼,许友毅;《现代妇女(医学前沿)》;20140331(第3期);第101页右栏第2段，第101页左栏第3段 * |
| 苦丁茶化学成分研究进展;沈强,司辉清,于洋;《茶业通报》;20100331;第32卷(第1期);摘要和第1.8节 * |
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