CN106418554B - Method for preparing soluble dietary fiber and mannan oligosaccharide by hydrolyzing guar gum with complex enzyme - Google Patents
Method for preparing soluble dietary fiber and mannan oligosaccharide by hydrolyzing guar gum with complex enzyme Download PDFInfo
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- 230000003301 hydrolyzing effect Effects 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 26
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- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
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- 239000003513 alkali Substances 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical group OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/12—Disaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a method for preparing soluble dietary fiber and mannan oligosaccharide by hydrolyzing guar gum with complex enzyme, which comprises the following steps: mixing guar gum solution, mannase and galactosidase, hydrolyzing, filtering, decolorizing, ion exchanging and concentrating; precipitating with anhydrous ethanol and drying. The invention provides a source for soluble dietary fiber; by utilizing the preparation method provided by the invention, the intermediate product, namely the soluble dietary fiber syrup containing galactomannan-oligosaccharides can be prepared, and the weight average molecular weight of the syrup is about 13700 Da; the method provided by the invention has high hydrolysis rate and galactomannan conversion rate, the product is easy to separate, the weight average molecular weight of the prepared soluble dietary fiber is about 14600Da, and the polymerization degree of mannan oligosaccharide is between 2 and 5.
Description
Technical Field
The invention belongs to the field of food processing, and particularly relates to a method for preparing soluble dietary fiber and mannan oligosaccharide by hydrolyzing guar gum with complex enzyme.
Background
Galactomannans typically comprise a mannan backbone formed by β -1, 4-glycosidic linkages and galactose residue side chains formed by α -1, 6-glycosidic linkages, and are found in a wide variety of plant cell walls and endosperm (Thombare et al international Journal of biological Macromolecules,2016,88: 361-372). Guar gum is a natural vegetable gum derived from guar (Cyamopsis tetragonolobus), has various excellent characteristics of high viscosity, good water solubility, acid and alkali resistance, high temperature resistance and the like, is widely applied to various industries, and mainly comprises galactomannan (Thombare et al. International Journal of Biological Macromolecules,2016,88: 361-. The proportion of mannose and galactose residues in galactomannans from different sources is usually different, the galactomannan from guar gum has a high content of galactose side chains, which is significantly higher than that of galactomannans from locust bean gum, and these side chains tend to be arranged centrally and orderly (Malgas et al enzyme and Microbial Technology,2015,70: 1-8; mccleary. carbohydrate research,1985,139: 237-.
Due to this complex multi-branched structure, the complete degradation of galactomannans in guar gum requires the co-action of several glycoside hydrolases, such as β -mannanase (EC 3.2.1.78), β -mannosidase (EC 3.2.1.25) and α -galactosidase (EC 3.2.1.22) etc. (Moreira et al applied Microbiology and dbio technology,2008,79: 165-. Of the most important, is beta-mannanase, which is capable of specifically and randomly hydrolyzing beta-1, 4-glycosidic bonds in the galactomannan backbone to produce low molecular weight oligosaccharides; in addition, α -galactosidase can cleave galactose side chains linked by α -1, 6-glycosidic linkages, and also plays an important role in the degradation of guar gum. In the CAZy database, the beta-mannanases belong to glycoside hydrolase families 5, 26, 113 and 134, respectively, and the alpha-galactosidases belong to glycoside hydrolase families 4, 27, 31, 36, 57, 97 and 110, respectively. Among them, α -galactosidase from family 27 can hydrolyze galactose side chains directly from galactomannan, thereby more exposing the mannanase site on galactomannan to facilitate further degradation of galactomannan by β -mannanase (Malgas et al enzyme and microbiological technology,2015,70: 1-8). Therefore, the method for synergistically hydrolyzing the guar gum by using the beta-mannase and the alpha-galactosidase is more suitable for hydrolyzing the guar gum to prepare the mannan oligosaccharide and the soluble dietary fiber.
Dietary fiber is a polysaccharide that is neither digested nor absorbed by the gastrointestinal tract, nor produces energy. The soluble dietary fiber refers to low molecular weight polysaccharide with polymerization degree of more than 10, which is soluble and not digested and absorbed by human body. The substances can stimulate intestinal peristalsis, facilitate feces discharge, and prevent constipation, rectal cancer, hemorrhoid and lower limb varicosis; can be used for preventing cardiovascular diseases such as atherosclerosis and coronary heart disease; preventing the formation of gallstones; the product has satiety effect, is beneficial to obesity patients, and can be used as weight reducing food; improving sugar tolerance, regulating blood sugar level of diabetes patients, and can be used as food for diabetes patients; improving intestinal flora, and preventing intestinal cancer and appendicitis.
Generally, alcohol precipitation is an effective means for separating and purifying polysaccharides (Jian et al. food Hydrocolloids,2014,40: 115-. The soluble dietary fiber can be precipitated by high-concentration ethanol, and the mannooligosaccharide can be dissolved in the high-concentration ethanol, so that the dietary fiber and the oligosaccharide can be separated by the method.
The hydrolysis of guar gum is reported less, wherein Chinese patent application with application number of 201510174562.2 discloses a method for preparing galacto-oligosaccharide by degrading guar gum through three enzymes of beta-mannase, xylanase and endoglucanase; the Chinese patent application with the application number of 201510175963.X discloses a method for preparing galacto-oligosaccharide by degrading guar gum through three enzymes of beta-mannase, xylanase and cellulase in a composite manner; the Chinese patent application with the application number of 201510175978.6 discloses a method for preparing galacto-oligosaccharides by compositely degrading guar gum by utilizing beta-mannase, endoglucanase and cellobiase, the invention patents all disclose the preparation of galacto-oligosaccharides, and do not relate to the preparation of soluble dietary fibers, and at present, reports on the preparation of soluble dietary fibers by utilizing the guar gum are not found.
Disclosure of Invention
The invention provides a method for preparing soluble dietary fiber and mannooligosaccharide by hydrolyzing guar gum with complex enzyme, which has the advantages of high hydrolysis rate and galactomannan conversion rate, easy product separation, about 13700Da weight average molecular weight of final syrup, about 14600Da weight average molecular weight of soluble dietary fiber and 2-5 polymerization degree of mannooligosaccharide.
The purpose of the invention is realized by the following technical scheme:
a method for preparing soluble dietary fiber and mannan oligosaccharide by hydrolyzing guar gum with complex enzyme comprises the following steps:
s1: mixing guar gum solution, mannase and galactosidase, hydrolyzing, inactivating in boiling water bath, and cooling to room temperature to obtain enzymatic hydrolysate;
s2: filtering, decoloring, ion exchanging and concentrating the enzymolysis liquid obtained in the step S1 to obtain syrup;
s3: adding absolute ethyl alcohol into the concentrated solution in the step S2, standing, centrifuging, and respectively collecting precipitate and supernate;
s4: washing the precipitate obtained in the step S3 with 80% (V/V) ethanol, and drying to obtain soluble dietary fiber;
s5: and (5) concentrating and drying the supernatant obtained in the step (S3) to obtain the mannose oligosaccharide powder.
Preferably, the ratio of guar gum to mannanase is: and adding 100-2000U of mannase into each gram of guar gum.
Preferably, the ratio of guar gum to galactosidase is: and adding 0-400U of galactosidase into each gram of guar gum.
Preferably, the concentration of the guar gum solution is 1-10%.
Preferably, the solvent of the guar gum is distilled water or a citric acid buffer.
Preferably, in the step S1, the temperature of the hydrolysis is 30 to 70 ℃.
Preferably, in the step S1, the hydrolysis time is 1-24 h.
Preferably, in the step S2, the weight average molecular weight of the syrup is 13700 Da.
Preferably, in the step S4, the weight average molecular weight of the soluble dietary fiber is 14600 Da.
Preferably, in the step S5, the polymerization degree of the mannooligosaccharides is 2 to 5.
Compared with the prior art, the invention has at least the following advantages:
1. the soluble dietary fiber is prepared by hydrolyzing guar gum with the complex enzyme, so that a source is provided for the soluble dietary fiber;
2. the method provided by the invention can simultaneously prepare the soluble dietary fiber and the mannan oligosaccharide, which can achieve two purposes;
3. by utilizing the preparation method provided by the invention, the intermediate product, namely the soluble dietary fiber syrup containing galactomannan-oligosaccharides can be prepared, and the weight average molecular weight of the syrup is about 13700 Da;
4. the method for preparing the soluble dietary fiber and the mannan by hydrolyzing the guar gum with the complex enzyme has the advantages of high hydrolysis rate and galactomannan conversion rate, easy product separation, weight average molecular weight of the prepared soluble dietary fiber of about 14600Da, and polymerization degree of mannan-oligosaccharide of between 2 and 5.
Drawings
FIG. 1 shows the viscosity of crude sugar solution and the yield of reducing sugar obtained by complex enzyme hydrolysis of guar gum at different hydrolysis times;
FIG. 2 is a process flow diagram for the production of mannooligosaccharide powder and soluble dietary fiber;
FIG. 3 is a gel exclusion chromatography analysis of the hydrolysate.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments.
The experimental procedures used in the following examples are all conventional procedures unless otherwise specified.
Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
The mannanase used in the examples below was derived from Mucor miehei (Katrolia et al. journal of Agricultural and Food Chemistry,2013,61: 394-.
A method for preparing soluble dietary fiber and mannan oligosaccharide by hydrolyzing guar gum with complex enzyme comprises the following steps:
s1: mixing guar gum solution, mannase and galactosidase, hydrolyzing, inactivating in boiling water bath after enzymolysis, and cooling to room temperature to obtain enzymatic hydrolysate;
s2: filtering, decoloring, ion exchanging and concentrating the enzymolysis liquid obtained in the step S1 to obtain syrup;
s3: adding absolute ethyl alcohol into the concentrated solution in the step S2, standing, centrifuging, and respectively collecting precipitate and supernate;
s4: washing the precipitate obtained in the step S3 with 80% (V/V) ethanol, and drying to obtain soluble dietary fiber;
s5: and (5) concentrating and drying the supernatant obtained in the step (S3) to obtain the mannose oligosaccharide powder.
As a preferred embodiment of this embodiment, the mixing ratio of guar gum and mannanase is: and adding 100-2000U of mannase into each gram of guar gum.
As a preferred embodiment of this embodiment, the mixing ratio of guar gum and galactosidase is: and adding 0-400U of galactosidase into each gram of guar gum.
As a preferred embodiment of this example, the concentration of the guar gum solution is 1% to 10%.
As a preferred embodiment of this example, the solvent of guar gum is distilled water or citric acid buffer.
In a preferred embodiment of this embodiment, in step S1, the temperature of hydrolysis is 30 to 70 ℃.
In a preferred embodiment of this embodiment, in step S1, the hydrolysis time is 1 to 24 hours.
In a preferred embodiment of this embodiment, in step S2, the weight average molecular weight of the syrup is 13700 Da.
In a preferred embodiment of this embodiment, in step S4, the weight average molecular weight of the soluble dietary fiber is 14600 Da.
In a preferred embodiment of this embodiment, in step S5, the polymerization degree of the mannooligosaccharides is 2 to 5.
Example 2 different amounts of mannanase complex hydrolyzed guar gum
Weighing 5g of guar gum, completely dissolving in 100mL of distilled water (the distilled water can be replaced by phosphate buffer or citrate buffer with pH of 7.0, etc.), respectively adding mannase according to the proportion of 100, 200, 400, 800, 1000 and 2000U/g of guar gum, hydrolyzing at 50 ℃ for 8h, and inactivating in boiling water bath for 10min after enzymolysis to obtain an enzymolysis solution. Centrifuging the obtained enzymolysis liquid at 10000rpm for 10min, collecting supernatant, namely crude sugar liquid, measuring the viscosity of the crude sugar liquid at 25 ℃ by using a DV-1 rotational viscometer, measuring the content of reducing sugar in the crude sugar liquid by using a 3, 5-dinitrosalicylic acid method, and calculating the yield of the reducing sugar. The experimental results are shown in Table 1.
TABLE 1 viscosity of crude sugar solution and reducing sugar yield obtained by hydrolyzing guar gum with different amounts of added mannanase
| Amount of enzyme added (U/g) | 100 | 200 | 400 | 800 | 1000 | 2000 |
| Viscosity of crude sugar solution (mPa. s) | 83.1 | 69.2 | 55.8 | 46.4 | 37.1 | 35.9 |
| Reducing sugar yield (%) | 7.3 | 11.5 | 15.6 | 18.1 | 19.4 | 20.0 |
As can be seen from Table 1, the guar gum is hydrolyzed by different addition amounts of the mannase, the viscosity of the crude sugar solution obtained by composite hydrolysis of the guar gum is gradually reduced along with the increase of the addition amount of the mannase, and the yield of reducing sugar is higher and higher. When the addition amount of the mannase reaches 1000U/g, the viscosity of the crude sugar solution is not obviously reduced, and the yield of reducing sugar is not obviously increased, so that the addition amount of the mannase is 1000U/g of hydrolyzed guar gum.
Example 3 hydrolysis of guar Gum with alpha-galactosidase and mannanase combinations at different addition levels
Weighing 5g of guar gum, dissolving in 100mL of distilled water (the distilled water can be replaced by phosphate buffer or citric acid buffer with pH of 7.0, etc.), adding mannase according to the proportion of 1000U/g of guar gum, respectively adding galactosidase according to the proportion of 0, 100, 200 and 400U/g of guar gum, hydrolyzing at 50 ℃ for 8h, and inactivating in boiling water bath for 10min after enzymolysis to obtain an enzymolysis solution. Centrifuging the obtained enzymolysis liquid at 10000rpm for 10min, collecting supernatant, namely crude sugar liquid, measuring the viscosity of the crude sugar liquid at 25 ℃ by using a DV-1 rotational viscometer, measuring the content of reducing sugar in the crude sugar liquid by using a 3, 5-dinitrosalicylic acid method, and calculating the yield of the reducing sugar.
The viscosity and reducing sugar yield of the obtained crude sugar solution are shown in table 2 after guar gum is hydrolyzed by compounding alpha-galactosidase with different addition amounts and mannase of 1000U/g. TABLE 2 viscosity of crude sugar solution and reducing sugar yield obtained by hydrolyzing guar gum with different galactosidase addition amounts and mannase
| Amount of enzyme added (U/g) | 0 | 100 | 200 | 400 |
| Viscosity of crude sugar solution (mPa. s) | 35.2 | 32.4 | 30.1 | 29.8 |
| Reducing sugar yield (%) | 19.9 | 29.9 | 34.5 | 35.9 |
As can be seen from Table 2, the guar gum hydrolyzed by combining alpha-galactosidase with 1000U/g mannase in different addition amounts has the advantages that the viscosity of the obtained crude sugar solution is reduced but is not obvious along with the continuous increase of the addition amount of the alpha-galactosidase, but the reducing sugar yield is increased gradually. When the addition amount of the galactosidase is between 0 and 2000U, the yield of reducing sugar is increased from 19.9 percent to 35.9 percent, and the viscosity of the crude sugar solution is reduced from 35.2 mPas to 29.8 mPas. The optimum amount of alpha-galactosidase to be added is 200U/g, considering cost.
Example 4 Experimental results of Complex enzyme hydrolysis of guar Gum at different hydrolysis time
Weighing 5g of guar gum, dissolving the guar gum in 100mL of distilled water, adding 1000U/g of mannase and 200U/g of galactosidase according to the proportion of the guar gum, respectively placing the mixture in a 50 ℃ environment for hydrolysis for 1, 2, 4, 8, 12 and 24 hours, inactivating the mixture in a boiling water bath for 10min after enzymolysis to obtain an enzymolysis solution, centrifuging the obtained enzymolysis solution at 10000rpm for 10min, and collecting a supernatant to obtain a crude sugar solution. And measuring the viscosity of the crude sugar solution at 25 ℃ by using a DV-1 rotational viscometer, determining the content of reducing sugar in the crude sugar solution by using a 3, 5-dinitrosalicylic acid method, and calculating the yield of the reducing sugar.
The viscosity of the crude sugar solution obtained by complex enzyme hydrolysis of guar gum at different hydrolysis time and the yield of reducing sugar are shown in figure 1. In the figure, black squares indicate the yield of reducing sugars, and open squares indicate the viscosity of the crude sugar solution. The results show that the viscosity of the crude sugar solution rapidly decreases and the reducing sugar yield gradually increases as the enzymolysis time is gradually prolonged. After 5g of guar gum is subjected to compound enzymolysis for 1-24h, the yield of the reducing sugar in the crude sugar solution reaches 36.1%, and the viscosity of the crude sugar solution is reduced to 31.4.
EXAMPLE 5 production of soluble dietary fiber syrup containing mannooligosaccharides
As shown in figure 2, 18L of distilled water is injected into a 20L enzymolysis tank, 900g of guar gum is added, mannase is added according to the proportion of 1000U/g to guar gum, galactosidase is added according to the proportion of 200U/g to guar gum, the enzymolysis tank is heated to 50 ℃ for hydrolysis for 8h, and the enzymolysis tank is cooled to room temperature after enzyme deactivation at 100 ℃. Discharging the enzymolysis solution, performing plate-frame filtration (other filtration methods can be selected), adding diatomaceous earth for assisting filtration, wherein the addition amount of diatomaceous earth is 3kg/m2(ii) a Collecting filtrate, decolorizing with active carbon with the addition of 0.5% (m/v), and keeping the temperature at 80 deg.C for 30 min; filtering to remove active carbon after decolorization, and performing ion exchange treatment according to the order of cation, anion and cation resin, wherein the cation resin is 001 × 7, and the anion resin is D301; concentrating by membrane or rotary evaporation after ion exchange treatment. Concentrating to obtain soluble dietary fiber syrup containing mannan oligosaccharide.
The soluble dietary fiber syrup containing the mannan oligosaccharide is produced by hydrolyzing guar gum with complex enzyme, and the yield of the soluble dietary fiber syrup is respectively 87.9%, 83.5%, 81.7%, 77.9% and 76.2% after the steps of enzymolysis, filtration, decoloration, ion exchange and concentration are calculated.
Weighing 10mg of soluble dietary fiber syrup containing mannan oligosaccharide, and performing vacuum freeze drying to obtain a powdery product.
A sample of 6mg of the lyophilized powder was dissolved in 3mL of distilled water, filtered through a 0.22 μm filter, and analyzed by gel exclusion chromatography. The chromatographic column is PL aquagel-OH MIXED (7.5mm I.D. times.30 cm), the column temperature is 40 deg.C, and the mobile phase is 0.1MNaNO3. Dextran with different molecular weights is used as a standard substance.
The results of gel exclusion chromatography of the hydrolysate are shown in FIG. 3. As can be seen from FIG. 3, the weight average molecular weight of the hydrolyzate was 13700Da and the dispersion index was 10.33.
EXAMPLE 6 production of mannooligosaccharide powder and soluble dietary fiber
As shown in fig. 2, a soluble dietary fiber syrup containing mannooligosaccharides was prepared according to the method in example 5.
Adding anhydrous ethanol into soluble dietary fiber syrup containing mannan oligosaccharide until ethanol concentration reaches 80% (V/V), standing for 30min, centrifuging at 10000rpm for 5min, and collecting precipitate and supernatant respectively. Washing the precipitate with 80% (V/V) ethanol for 3 times, vacuum freeze drying to obtain soluble dietary fiber, rotary evaporating the supernatant, concentrating, and freeze drying to obtain mannooligosaccharide powder. The yield of the dietary fiber and the mannan oligosaccharide powder is 55.4 percent and 26.9 percent respectively, the weight average molecular weight of the dietary fiber is 14600Da, and the polymerization degree of the mannan oligosaccharide is between 2 and 5.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are also included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (5)
1. A method for preparing soluble dietary fiber and mannan oligosaccharide by hydrolyzing guar gum with complex enzyme is characterized by comprising the following steps:
s1: mixing guar gum solution, mannase and galactosidase, hydrolyzing, inactivating in boiling water bath, and cooling to room temperature to obtain enzymatic hydrolysate; the ratio of guar gum to mannanase is: adding 1000U of mannase into each gram of guar gum, wherein the proportion of the guar gum to the galactosidase is as follows: adding 200U of galactosidase into each gram of guar gum;
s2: filtering, decoloring, ion exchanging and concentrating the enzymolysis liquid obtained in the step S1 to obtain syrup;
s3: adding absolute ethyl alcohol into the syrup in the step S2, standing, centrifuging, and respectively collecting precipitate and supernate;
s4: washing the precipitate obtained in the step S3 with 80% (V/V) ethanol, and drying to obtain soluble dietary fiber;
s5: concentrating and drying the supernatant in the step S3 to obtain mannooligosaccharide powder, wherein the weight average molecular weight of the syrup is 13700Da, in the step S4, the weight average molecular weight of the soluble dietary fiber is 14600Da, and in the step S5, the polymerization degree of the mannooligosaccharide is 2-5.
2. The method for preparing soluble dietary fiber and mannooligosaccharide by hydrolyzing guar gum with complex enzyme according to claim 1, wherein the concentration of the guar gum solution is 1-10%.
3. The method for preparing soluble dietary fiber and mannooligosaccharide by hydrolyzing guar gum with complex enzyme according to claim 1, wherein the solvent of guar gum is distilled water or citric acid buffer solution.
4. The method for preparing soluble dietary fiber and mannooligosaccharide by hydrolyzing guar gum with complex enzyme according to claim 1, wherein the hydrolysis temperature is 30-70 ℃ in the step S1.
5. The method for preparing soluble dietary fiber and mannooligosaccharide by hydrolyzing guar gum with complex enzyme according to claim 1, wherein the hydrolysis time is 1-24h in the step S1.
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