CN106399557B - The molecular specificity labeled primers and discrimination method of straw berry tomato - Google Patents
The molecular specificity labeled primers and discrimination method of straw berry tomato Download PDFInfo
- Publication number
- CN106399557B CN106399557B CN201611006388.1A CN201611006388A CN106399557B CN 106399557 B CN106399557 B CN 106399557B CN 201611006388 A CN201611006388 A CN 201611006388A CN 106399557 B CN106399557 B CN 106399557B
- Authority
- CN
- China
- Prior art keywords
- primer
- straw berry
- berry tomato
- st3msjr
- st3msjf
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000009230 Physalis pubescens Nutrition 0.000 title claims abstract description 42
- 235000004876 Physalis pruinosa Nutrition 0.000 title claims abstract description 40
- 244000172412 Physalis pruinosa Species 0.000 title claims abstract description 35
- 238000012850 discrimination method Methods 0.000 title description 3
- 238000000034 method Methods 0.000 claims abstract description 21
- 235000002731 Mimulus guttatus Nutrition 0.000 claims abstract description 15
- 238000011144 upstream manufacturing Methods 0.000 claims abstract description 4
- 238000001962 electrophoresis Methods 0.000 claims description 13
- 238000012408 PCR amplification Methods 0.000 claims description 9
- 238000001514 detection method Methods 0.000 claims description 9
- 238000004925 denaturation Methods 0.000 claims description 6
- 230000036425 denaturation Effects 0.000 claims description 6
- 230000004087 circulation Effects 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 238000000137 annealing Methods 0.000 claims description 3
- 239000012154 double-distilled water Substances 0.000 claims description 3
- 244000016966 Mimulus guttatus Species 0.000 claims description 2
- 244000171805 Mimulus langsdorfii Species 0.000 abstract description 12
- 240000006285 Physalis pubescens Species 0.000 abstract description 9
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 239000006101 laboratory sample Substances 0.000 abstract description 2
- 230000035945 sensitivity Effects 0.000 abstract description 2
- 108020004414 DNA Proteins 0.000 description 18
- 244000177154 Solanum pseudocapsicum Species 0.000 description 11
- 235000000340 Solanum pseudocapsicum Nutrition 0.000 description 11
- 235000001978 Withania somnifera Nutrition 0.000 description 11
- 239000000523 sample Substances 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 235000013399 edible fruits Nutrition 0.000 description 6
- 239000000499 gel Substances 0.000 description 6
- 239000002253 acid Substances 0.000 description 5
- 238000013461 design Methods 0.000 description 5
- 238000000605 extraction Methods 0.000 description 5
- 239000012634 fragment Substances 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- 238000012163 sequencing technique Methods 0.000 description 4
- 239000002002 slurry Substances 0.000 description 4
- GVGLGOZIDCSQPN-PVHGPHFFSA-N Heroin Chemical compound O([C@H]1[C@H](C=C[C@H]23)OC(C)=O)C4=C5[C@@]12CCN(C)[C@@H]3CC5=CC=C4OC(C)=O GVGLGOZIDCSQPN-PVHGPHFFSA-N 0.000 description 3
- 240000005569 Merope angulata Species 0.000 description 3
- 241000244026 Physalis alkekengi var. franchetii Species 0.000 description 3
- 244000093225 Physalis minima Species 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 238000007400 DNA extraction Methods 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 241001106044 Physalis Species 0.000 description 2
- 241000208292 Solanaceae Species 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000003147 molecular marker Substances 0.000 description 2
- 239000004570 mortar (masonry) Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241001164374 Calyx Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 241000207860 Mimulus <angiosperm> Species 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 208000034510 Parotid gland inflammation Diseases 0.000 description 1
- 206010034038 Parotitis Diseases 0.000 description 1
- 240000004001 Physalis peruviana Species 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 206010000269 abscess Diseases 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000027939 micturition Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The present invention relates to identify straw berry tomato molecular specificity labeled primers and it is a kind of using the specific primer to straw berry tomato carry out quickly reflect method for distinguishing.The primer sequence are as follows: upstream primer ST3MSJF:5 '-ATTGATAGGGTAAACCATG-3；Downstream primer ST3MSJR:5 '-CTGTAATAAAACAAAAGCG-3 '.Straw berry tomato can quickly be identified by conventional PCR method using specific primer ST3MSJF/ST3MSJR.Laboratory sample dosage of the present invention is few；As a result accurate, high sensitivity, ST3MSJF/ST3MSJR are straw berry tomato specificity amplimer, are negative reaction if other monkey flower samples；Method is easy, is detected using Standard PCR technology, time-consuming short, can be completed within 1st.
Description
Technical field
The invention belongs to identify the technology neck of straw berry tomato Physalis pubescens L. using molecular biology method
Domain, be related to it is a kind of identify straw berry tomato specificity labeled primers and it is a kind of using the specific primer to straw berry tomato carry out fastly
Speed mirror method for distinguishing.
Background technique
Straw berry tomato (Physalis pubescens L.), alias Huang mushroom creeping plant, Mushroom ma are Solanaceae (Solanaceae) acid
Slurry belongs to (Physalis) annual herb plant.It originates in America, in China Jilin, Heilungkiang, Liaoning and the Eastern Inner Mongolia southeast
Fringe region have cultivation or ease be it is wild, be born in by meadow or field wing more.Straw berry tomato is a kind of very important medical, edible
Have both plant.As folk tradition Chinese medicine simply, straw berry tomato is included earliest in Shennong's Herbal, is listed as middle product, band
Calyx fruit or herb are known as Chinese lantern, and nature and flavor acid is flat, enters lung channel, have effects that clearing heat and detoxicating, diuresis hemostasis, can cure the parotid gland
Inflammation, abscess of throat, difficult urination and cough with lung heat, etc..Other than medicinal efficacy, straw berry tomato is also act as high-grade novel nourishing
Health care " herbal fruit ", fruit faint scent is palatable, sweet and delicious, vitamin C rich in, carrotene, calcium, iron in fruit
It is full of nutrition Deng the amino acid that 20 several mineral materials and 18 kinds of needed by human body are wanted, it is favored by people.In addition, physalis pubescens fruit
Also processable that fruit juice, candied fruit, can etc., unique flavor is made, adult children are fond of, are suitable for people of all ages.Monkey flower (hair
Wintercherry Physalis pubescens, small wintercherry P.minima, Ku Zhi P.angulata and wintercherry Physalis alkekengi
Var.franchetii morphological feature) is extremely similar, is difficult using conventional sorting methods by straw berry tomato and other Physalis
Similar plant distinguishes.Therefore, by other monkey flowers be mistakenly considered straw berry tomato carry out using the case where happen occasionally, this is hair
The safe handling and protection of wintercherry resource bring very big difficulty.
DNA molecular marker especially specific molecular marker technology makes up and overcomes some defects of conventional sorting methods
And problem, it can be analyzed by gene order comparison in difference and provide direct evidence for plant identification, genealogical classification.This patent is first
SCoT molecular marking technique is first used, monkey flower SCoT finger-print is obtained using round pcr.By largely screening examination
It tests, obtains straw berry tomato specific band, by the methods of gel extraction, TA clone, sequencing, exploitation devises fast for straw berry tomato
Speed identify specificity labeled primers, for straw berry tomato germ plasm resource it is accurate identify and protect provide effective molecular engineering according to
According to.So far, there has been no molecular specificity markers to be applied to the research report that straw berry tomato identifies.
Summary of the invention
First purpose of the invention is in view of the deficiencies of the prior art, to provide the molecular specificity labeled primers of straw berry tomato
(ST3MSJF/ST3MSJR), the specificity labeled primers sequence is as follows:
Upstream primer ST3MSJF:5 '-ATTGATAGGGTAAACCATG-3 ', as shown in SEQ ID NO.1；
Downstream primer ST3MSJR:5 '-CTGTAATAAAACAAAAGCG-3 ', as shown in SEQ ID NO.2.
Primer combination is, by a large amount of screening experiments, to be marked using SCoT using Standard PCR technology and obtain straw berry tomato spy
Specific DNA-fragments obtain the DNA sequence dna by gel extraction, TA clone, sequencing, design straw berry tomato specificity on this basis
Labeled primer carries out PCR amplification with the primer pair monkey flower, only reacts with straw berry tomato sample DNA, obtain 1479bp
The specific fragment of size, without with other monkey flower example reactions.It is anti-by Standard PCR with the specific primer
It can should quickly identify hirsutic acid slurry samples.
A second object of the present invention is to provide the methods that above-mentioned molecular specificity labeled primers identify straw berry tomato.This method
Are as follows: specificity amplification primer is used as using above-mentioned primer combination (ST3MSJF/ST3MSJR), with monkey flower genome to be measured
DNA is template, carries out PCR amplification, carries out electrophoresis detection to amplified production, if it is 1479bp size that molecular weight, which occurs, in electrophoresis result
Specific fragment, then monkey flower sample to be measured is straw berry tomato, on the contrary then be not.
Specific the method for the invention is as follows:
(1) extraction of genomic DNA: the fresh blade 100mg of clip monkey flower sample to be measured is put into mortar, immediately plus
Enter liquid nitrogen grinding to powder, then (is purchased from the raw work biology in Shanghai using UNIQ-10 pillar plant genome DNA extraction agent box
Engineering Co., Ltd) genomic DNA is extracted, gained DNA carries out electrophoresis detection with 0.8% agarose gel, and with ultraviolet spectrometry light
Degree meter detectable concentration, is diluted to 50ng/ μ l.
(2) genomic DNA extracted using step (1) is template, with the molecular specificity labeled primers (ST3MSJF/
ST3MSJR it) is used as amplimer, carries out PCR amplification.
PCR amplification system (20 μ l of total volume): 2 μ l 10 × Buffer, 2 μ l MgCl2(25mM), 0.8 μ l dNTPs
(10mM), 1 μ l primer ST3MSJF (10 μM), 1 μ l primer ST3MSJR (10 μM), 1 μ l template DNA (50ng/ μ l), 0.5 μ l
Taq enzyme (2U/ μ l), 11.7 μ l ddH2O。
PCR response procedures: 94 DEG C of initial denaturation 5min；(94 DEG C of denaturation 50s, 56 DEG C of annealing 50s, 72 DEG C extend 35 circulations
1.5min)；72 DEG C of extension 10min.
(3) electrophoresis detection is carried out to step (2) PCR product using 1.5 ﹪ Ago-Gels, taken pictures through gel imaging system
Detection, if the DNA band that molecular weight is 1479bp size occurs in electrophoresis result, sample to be tested is straw berry tomato, on the contrary then be not.
Laboratory sample dosage of the present invention is few；As a result accurate, high sensitivity, ST3MSJF/ST3MSJR are straw berry tomato specificity
Amplimer is negative reaction if other monkey flower samples；Method is easy, is detected using Standard PCR technology,
It is time-consuming short, it can be completed within 1st.
Detailed description of the invention
Fig. 1 be using the specificity labeled primers ST3MSJF/ST3MSJR that designs of the present invention to monkey flower sample into
Electrophoretogram after row PCR amplification, wherein M is DNA molecular amount standard marker DL2000；Channel 1~4: small wintercherry
P.minima；5~13: Ku Zhi P.angulata；14~17: wintercherry P.alkekengi var.franchetii；18~20: hair
Wintercherry P.pubescens.Electrophoretogram shows that only straw berry tomato sample amplification goes out the specific DNA item that molecular weight is 1479bp size
Band.
Specific embodiment
The present invention more can fast and accurately identify hirsutic acid slurry samples from molecular level, by following embodiment to this
Invention is described further, but protection scope of the present invention is not limited to that:
Embodiment 1: straw berry tomato molecular specificity labeled primers exploitation design
1. the extraction of genomic DNA
The fresh blade 100mg of clip monkey flower sample is put into mortar, and wherein sample includes that small wintercherry (picks up from 1: Hebei
Tangshan, 2: Heze City, Shandong Province, 3: the peace of Anhui six, 4: Lishui of Zhejiang), Ku Zhi (5-6: Zhejiang Hangzhou, 7: Huanggang, 8: Yunnan Red
River, 9: Nanjing, 10: Taizhou of Zhejiang, 11: Wenzhou District of Zhejiang Province, 12: Jinhua, Zhejiang, 13: Shandong Dezhou), wintercherry (14: Liaoning Shen
Sun, 15-16: Dandong, 17: Jinan, Shandong Province) and straw berry tomato (18: Liaoning Shenyang, 19: Heilungkiang Zhaodong, 20: Jilin is long
Spring).Liquid nitrogen grinding is added immediately to powder, then (is purchased from using UNIQ-10 pillar plant genome DNA extraction agent box
Hai Shenggong bioengineering Co., Ltd) genomic DNA is extracted, gained DNA carries out electrophoresis detection with 0.8% agarose gel, is used in combination
UV spectrophotometer measuring concentration is diluted to 50ng/ μ l.For subsequent PCR amplification.
2. designing molecular specificity labeled primers
Screening experiment is expanded by a large amount of SCoT-PCR, is cloned by gel extraction, TA and sequencing obtains straw berry tomato spy
Specific nucleotide sequence, design obtains ST3MSJF/ST3MSJR primer sequence (ST3MSJF:5 '-on this basis
ATTGATAGGGTAAACCATG-3'；ST3MSJR:5 '-CTGTAATAAAACAAAAGCG-3 ').Primer is by the raw work biology in Shanghai
Engineering Co., Ltd's synthesis.
Embodiment 2: molecular specificity labeled primers PCR amplification and electrophoresis detection
With design synthesis primer ST3MSJF/ST3MSJR to different monkey flower samples (being specifically shown in Detailed description of the invention) into
Row PCR detection.
PCR reaction system (20 μ l of total volume): 2 μ l 10 × Buffer, 2 μ l MgCl2(25mM), 0.8 μ l dNTPs
(10mM), 1 μ l primer ST3MSJF (10 μM), 1 μ l primer ST3MSJR (10 μM), 1 μ l template DNA (50ng/ μ l), 0.5 μ l
Taq enzyme (2U/ μ l), 11.7 μ l ddH2O。
PCR response procedures: 94 DEG C of initial denaturation 5min；(94 DEG C of denaturation 50s, 56 DEG C of annealing 50s, 72 DEG C extend 35 circulations
1.5min)；72 DEG C of extension 10min.
Using 1.5 ﹪ Ago-Gels to PCR product carry out electrophoresis detection, electrophoretogram as shown in Figure 1, from Fig. 1 (in figure,
Channel M is DNA molecular amount standard marker DL2000；Channel 1~4: small wintercherry P.minima；5~13: Ku Zhi
P.angulata；14~17: wintercherry P.alkekengi var.franchetii；18~20: straw berry tomato P.pubescens) may be used
To find out, only straw berry tomato (channel 18~20) can amplify specific fragment, and the specific fragment of straw berry tomato is sent to sequencing,
Its sequence length is 1479bp.And other monkey flower samples do not amplify any band, this shows of the invention special
Property primer there is high specificity, therefore can be used for the quick identification of hirsutic acid slurry samples.
SEQUENCE LISTING
<110>Hangzhou Pedagogic University
<120>molecular specificity labeled primers and discrimination method of straw berry tomato
<130> 1
<160> 2
<170> PatentIn version 3.3
<210> 1
<211> 19
<212> DNA
<213>artificial synthesized
<400> 1
attgataggg taaaccatg 19
<210> 2
<211> 19
<212> DNA
<213>artificial synthesized
<400> 2
ctgtaataaa acaaaagcg 19
Claims (4)
1. the molecular specificity labeled primers of straw berry tomato, it is characterised in that the specific primer sequence is as follows:
Upstream primer ST3MSJF:5 '-ATTGATAGGGTAAACCATG-3 '；
Downstream primer ST3MSJR:5 '-CTGTAATAAAACAAAAGCG-3 '.
2. a kind of carry out fastly straw berry tomato using molecular specificity labeled primers ST3MSJF/ST3MSJR described in claim 1
Speed mirror method for distinguishing, it is characterised in that the method are as follows: using the molecular specificity labeled primers as amplimer, with to be measured
Monkey flower genomic DNA is template, carries out PCR amplification, electrophoresis detection, if the special of 1479bp size occurs in electrophoresis result
Property segment, then sample to be tested be straw berry tomato；
The molecular specificity labeled primers sequence are as follows:
Upstream primer ST3MSJF:5 '-ATTGATAGGGTAAACCATG-3 '；
Downstream primer ST3MSJR:5 '-CTGTAATAAAACAAAAGCG-3 '.
3. method according to claim 2, it is characterised in that PCR amplification system is by 2 μ l10 × Buffer, 2 μ l concentration
The MgCl of 25mM2, 0.8 μ l concentration is the dNTPs of 10mM, and the primer ST3MSJF that 1 μ l concentration is 10 μM, 1 μ l concentration is 10 μM
Primer ST3MSJR, 1 μ l concentration are the template DNA of 50ng/ μ l, and 0.5 μ l concentration is the Taq enzyme of 2U/ μ l, 11.7 μ l ddH2O structure
At.
4. method according to claim 2, it is characterised in that PCR response procedures: 94 DEG C of initial denaturation 5min；35 circulations,
In it is each circulation be 94 DEG C of denaturation 50s, 56 DEG C of annealing 50s, 72 DEG C of extension 1.5min；72 DEG C of extension 10min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611006388.1A CN106399557B (en) | 2016-11-16 | 2016-11-16 | The molecular specificity labeled primers and discrimination method of straw berry tomato |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611006388.1A CN106399557B (en) | 2016-11-16 | 2016-11-16 | The molecular specificity labeled primers and discrimination method of straw berry tomato |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106399557A CN106399557A (en) | 2017-02-15 |
| CN106399557B true CN106399557B (en) | 2019-04-30 |
Family
ID=59229944
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611006388.1A Active CN106399557B (en) | 2016-11-16 | 2016-11-16 | The molecular specificity labeled primers and discrimination method of straw berry tomato |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106399557B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109456967B (en) * | 2018-12-20 | 2021-08-03 | 杭州师范大学 | Specific nucleotide, labeled primer and identification method of physalis macrocarpa |
| CN113755635B (en) * | 2021-10-14 | 2023-09-29 | 杭州师范大学 | Specific nucleotide sequence of myxoplasma, labeled primer and identification method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105505928A (en) * | 2016-02-04 | 2016-04-20 | 杭州科兴生物化工有限公司 | Nucleotide sequence, specific primer and method for identifying physalis angulata |
-
2016
- 2016-11-16 CN CN201611006388.1A patent/CN106399557B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105505928A (en) * | 2016-02-04 | 2016-04-20 | 杭州科兴生物化工有限公司 | Nucleotide sequence, specific primer and method for identifying physalis angulata |
Non-Patent Citations (2)
| Title |
|---|
| Iden tification of Single-Copy Orthologous Genes between Physalis and Solanum lycopersi cum and Analys is of Genetic Diversity in Physalis Using Molecular Markers;Jingli Wei et al.;《PLOS ONE》;20121116;第7卷(第11期);全文 |
| 基于ITS2序列的茄科酸浆属植物的DNA分子鉴定;吴亚男 等;《中国实验方剂学杂志》;20160430;第22卷(第8期);全文 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106399557A (en) | 2017-02-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106521003B (en) | A kind of method identifying dendrobium candidum and its PCR primer used | |
| WO2019237906A1 (en) | Fluorescence pcr detection kit for identifying dendrobium officinale kimura et migo and use | |
| CN107557369A (en) | Thin shell mountain pecan Peach cultivars Nacono characteristic sequence, labeled primer and authentication method | |
| CN105505928B (en) | A kind of nucleotide sequence, specific primer and method differentiating Ku Zhi | |
| CN104450938A (en) | Ginseng identification method and special kit | |
| CN107557434A (en) | Thin shell mountain pecan Peach cultivars Van Deman characteristic sequence, labeled primer and authentication method | |
| CN106399557B (en) | The molecular specificity labeled primers and discrimination method of straw berry tomato | |
| CN105177148B (en) | The double PCR primer of two kinds of grape ulcer bacterium of detection and its application simultaneously | |
| CN108330163A (en) | Characteristic sequence, primer and the identification method of thin shell mountain pecan Peach cultivars Nacono and Sumner | |
| Li et al. | Three new Russula species in sect. Ingratae (Russulales, Basidiomycota) from southern China | |
| CN104073550A (en) | SCAR molecular mark for performing sex identification of siraidia grosvenorii | |
| CN110564885A (en) | Specific molecular markers for identifying Ganoderma sinense and Ganoderma lucidum strains, and identification method and application thereof | |
| CN112359135B (en) | Indel labeled primer of fritillaria taipaiensis and application thereof | |
| CN108796119A (en) | Specific molecular marker PCMI-M001 for Cathay poplar male seedling Rapid identification | |
| CN104232785B (en) | Oriental fruit moth fluorescent light PCR (Polymerase Chain Reaction) detection method and application | |
| CN105274245B (en) | A kind of method and its special primer pair for identifying David's-harp | |
| CN106636369A (en) | Molecular specific labeling primer of physalis minima and detection method | |
| CN107586866A (en) | Thin shell mountain pecan Peach cultivars Moore characteristic sequence, labeled primer and authentication method | |
| CN107630102A (en) | Pacify root of herbaceous peony PCR identification kits and authentication method | |
| CN103993070A (en) | SSR primer and method for purity identification of Linglong zucchini hybrid seed | |
| CN106701968A (en) | Primer for simultaneously detecting Botryosphaeria dothidea and Cryptosporella viticola and application of primer | |
| CN106480207B (en) | A kind of nucleotide sequence, specificity labeled primers and method identifying wintercherry | |
| KR101948389B1 (en) | Discriminating method of Codonopsis lanceolata and Adenophora triphylla using SSR marker | |
| CN107574255B (en) | Method for detecting fritillaria cirrhosa and fritillaria cirrhosa components by real-time fluorescence PCR | |
| CN108754015A (en) | Specific molecular marker PCMI-F001 for Cathay poplar female seedling Rapid identification |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |