CN106399548A - Tridacna mitochondrion 16S gene amplification primer and application thereof - Google Patents
Tridacna mitochondrion 16S gene amplification primer and application thereof Download PDFInfo
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- CN106399548A CN106399548A CN201610966874.1A CN201610966874A CN106399548A CN 106399548 A CN106399548 A CN 106399548A CN 201610966874 A CN201610966874 A CN 201610966874A CN 106399548 A CN106399548 A CN 106399548A
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- tridacna
- primer
- concha tridacna
- tridacna squamosa
- squamosa
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- 241000512272 Tridacna Species 0.000 title claims abstract description 15
- 230000004544 DNA amplification Effects 0.000 title claims abstract description 14
- 210000003470 mitochondria Anatomy 0.000 title claims abstract description 13
- 241001600158 Tridacna squamosa Species 0.000 claims abstract description 79
- 241000237502 Ostreidae Species 0.000 claims description 9
- 235000020636 oyster Nutrition 0.000 claims description 9
- 238000000246 agarose gel electrophoresis Methods 0.000 abstract description 5
- 241001600174 Hippopus hippopus Species 0.000 abstract description 2
- 241001600155 Tridacna crocea Species 0.000 abstract description 2
- 241001600159 Tridacna maxima Species 0.000 abstract description 2
- 238000003752 polymerase chain reaction Methods 0.000 abstract 2
- 239000000284 extract Substances 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 241000512273 Tridacnidae Species 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000001502 gel electrophoresis Methods 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 235000015170 shellfish Nutrition 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 235000005956 Cosmos caudatus Nutrition 0.000 description 2
- 244000293323 Cosmos caudatus Species 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 239000010437 gem Substances 0.000 description 2
- 230000000877 morphologic effect Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000012772 sequence design Methods 0.000 description 2
- 241000237519 Bivalvia Species 0.000 description 1
- 235000014653 Carica parviflora Nutrition 0.000 description 1
- 206010008190 Cerebrovascular accident Diseases 0.000 description 1
- 241000243321 Cnidaria Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000237852 Mollusca Species 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 241001600160 Tridacna derasa Species 0.000 description 1
- 241001600163 Tridacna gigas Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 239000011013 aquamarine Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 238000012942 design verification Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000024241 parasitism Effects 0.000 description 1
- 238000013081 phylogenetic analysis Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
Abstract
The invention discloses a tridacna mitochondrion 16S gene amplification primer and application thereof. A universal forward primer F is designed on the basis of a tridacna 16S sequence in a Genbank database, specific reverse primers are designed aiming at different tridacna including Tri_squ_YZ_R (tridacna squamosa), Tri_max_YZ_R (tridacna maxima), Tri_gig_YZ_R (tridacna cookiana), Tri_cro-YZ_R (tridacna crocea) and Hip_hip_YZ_R (hippopus hippopus), PRC (polymerase chain reaction) is performed under a 50uL system, and finally types of tridacna can be identified through a strip of agarose gel electrophoresis. Tridacna species can be identified quickly and accurately by adopting the tridacna mitochondrion 16S gene amplification primer.
Description
Technical field
The invention belongs to gene engineering technology field, it is related to a kind of gene amplification primer, more particularly, to one kind is based on Concha Tridacna squamosa
Mitochondrion 16S gene amplification primer and apply identification Concha Tridacna squamosa species.
Background technology
The closed shell flesh of Concha Tridacna squamosa and sufficient block are very loose, are delicious Cooking Materials of fine quality；In Chinese medicine, the shell of Concha Tridacna squamosa shellfish
It is considered, with Margarita, there is same curative effect；The ornamental value of Concha Tridacna squamosa essentially consists in two aspects, and one is with shell as raw material
Various shell carvings, pelletron etc., one is live body Concha Tridacna squamosa；Concha Tridacna squamosa is similar to other shellfishes, in the parasitism or the thorn that are subject to foreign body once in a while
Also Margarita can be generated when sharp；The gem that can flee from evil as getting rid of evils in the popular gem kind apoplexy due to endogenous wind of current Buddhism circle should first elect " Buddhism
Sapporo "；Concha Tridacna squamosa plays very important role in ocean, be evaluate the ecological whether health of coral reef important indicator it
One.In recent years because various places are excessively captured, cause Concha Tridacna squamosa resource to fall sharply, should take measures from now on to protect limited wild resource, and carry out
The fundamental biological knowledge of Concha Tridacna squamosa and artificial culture technology research, develop Concha Tridacna squamosa aquaculture, and to meet the consumption demand of people, Concha Tridacna squamosa will
It is a kind of marine commercial molluscs having very much breeding prospect.
Concha Tridacna squamosa is under the jurisdiction of Bivalvia, different tooth subclass, curtain clam mesh, is maximum bivalve shellfish in ocean.Find so far
And name have 2 to belong to totally 10 kinds, be mainly distributed on Pacific and Indian oceans, China has six kinds, be distributed in Taiwan and the South Sea, I
State lays special stress on protecting, in aqua-marine life register, Ku Shi Concha Tridacna squamosa is classified as one-level state guarantee marine organisms.
Using morphological characteristic, Concha Tridacna squamosa is carried out with species identification and phylogenetic analysis becomes abnormal difficult, especially for one
Nearly edge species a bit, simple interest morphological characteristic is difficult to make a distinction.
Content of the invention
It is an object of the invention to provide a kind of be based on Concha Tridacna squamosa mitochondrion 16S gene amplification primer, by PCR amplifying specific
16S fragment is differentiating Concha Tridacna squamosa species.Relative analyses US National Biotechnology Information center (NCBI, http://
Www.ncbi.nlm.nih.gov/) existing Concha Tridacna squamosa 16S sequence design specific primer in GenBank data, by repeating
Test is it is determined that the reverse primer of Concha Tridacna squamosa species can be differentiated, and finds out optimum reaction condition.
To achieve these goals, the technical scheme is that：There is provided and be based on Concha Tridacna squamosa mitochondrion 16S gene amplification primer
Thing：
F:GGCCTTTAATTGGGGTCTGGTATG
Tri_squ_YZ_R (squama Concha Tridacna squamosa primer):TCTAAGTATAATGGATCTTTGCAT
Tri_max_YZ_R (Tridacna (chamestrachea) maxima (R ding). primer):CATTTACTAAATATTCAAATCCCTC
Tri_gig_YZ_R (Ku Shi Concha Tridacna squamosa primer):TCTCTTTCTAATGGATCTTTGCTT
Tri_cro_YZ_R (sarranine Concha Tridacna squamosa primer):TCTTAGATAAAATGGATCTATGCGC
Hip_hip_YZ_R (Che oyster primer):CTTCTAACGGTTTTACAACATTAGA.
Using the Concha Tridacna squamosa mitochondrion 16S gene amplification primer of the present invention, can be rapid, accurately identify Concha Tridacna squamosa species.
Brief description
Fig. 1 is Che oyster pictorial diagram；
Fig. 2 is the agarose gel electrophoresis figure of Fig. 1 Concha Tridacna squamosa；
Fig. 3 is squama Concha Tridacna squamosa pictorial diagram；
Fig. 4 is the agarose gel electrophoresis figure of Fig. 3 Concha Tridacna squamosa；
Fig. 5 is sarranine Concha Tridacna squamosa pictorial diagram；
Fig. 6 is the agarose gel electrophoresis figure of Fig. 5 Concha Tridacna squamosa.
Specific embodiment
The screening technique of the Concha Tridacna squamosa mitochondrion 16S gene amplification primer of the present invention adopts four steps：A, in US National
Biotechnology Information center (NCBI, http://www.ncbi.nlm.nih.gov/) upper download China existing Tridacnidae sequence
Row, as shown in the sequence in sequence table；And according to Tridacnidae primers；B, filtered out by PCR and can preferably expand 16S
The forward primer of gene；C, from Concha Tridacna squamosa sequence obtained above, by compare find out specific sequence fragment design primer；d、
Specific primer using design enters performing PCR, the discriminating to Concha Tridacna squamosa species.
With reference to embodiment, the invention will be further described：
1. sample collecting：In 2015～2016, from the 50 of the coastal collection altogether in Chinese north and south Concha Tridacna squamosa 6 species of 2 genus
Individuality.
2. in US National Biotechnology Information center (NCBI, http://www.ncbi.nlm.nih.gov/) upper download
Existing 5 kinds of Concha Tridacna squamosa 16S sequences:Sarranine Concha Tridacna squamosa, big Concha Tridacna squamosa, squama Concha Tridacna squamosa, Tridacna (chamestrachea) maxima (R ding)., Che oyster.
3.DNA extracts：Extract the DNA of Concha Tridacna squamosa with Tiangeng marine animal tissue DNA extracts kit.
4. find the amplification preferable forward primer of 16S：By NCBI, find the 16S base of five kinds of Concha Tridacna squamosas in China's distribution
Because of sequence, devise the primer (explanation of Tridacnidae：Because no squama Concha Tridacna squamosa and other Concha Tridacna squamosa have obvious difference, need not pass through
Molecular tools are distinguished).The Concha Tridacna squamosa of selected part collection enters performing PCR amplification, PCR heat individual by positive anti-primer combination of two
Cycle annealing temperature range is 55 DEG C, picks out preferable F by 1% agarose gel electrophoresiies ratio.
5. binding specificity reverse primer differentiates Concha Tridacna squamosa species：
By the primer of aligned sequences design verification difference Concha Tridacna squamosa, as follows for specific sequence design reverse primer：
Tri_squ_YZ_R (squama Concha Tridacna squamosa primer):TCTAAGTATAATGGATCTTTGCAT
Tri_max_YZ_R (Tridacna (chamestrachea) maxima (R ding). primer):CATTTACTAAATATTCAAATCCCTC
Tri_gig_YZ_R (Ku Shi Concha Tridacna squamosa primer):TCTCTTTCTAATGGATCTTTGCTT
Tri_cro_YZ_R (sarranine Concha Tridacna squamosa primer):TCTTAGATAAAATGGATCTATGCGC
Hip_hip_YZ_R (Che oyster primer):CTTCTAACGGTTTTACAACATTAGA,
By reverse primer R and F combine, PCR thermal cycle annealing region be 60 DEG C, amplified production with 1% agar
Sugared electrophoresis is detected.
6. verify primer amplification condition：Above-mentioned F with checking R amplimer 50 μ L PCR reaction systems be：
Reaction condition:(three-step approach)
98 DEG C of 10sec, 60 DEG C of 5sec, 72 DEG C of 30sec, circulation 35 circle.
7. verify primer amplification result：Concha Tridacna squamosa is taken to be verified, as Figure of description 2,4,6 agarose gel electrophoresis figure institute
Show, first left hole is 2000Marker, corresponding reverse primer is respectively in the second hole to the 6th hole, Tri_gig_YZ_R
(Ku Shi Concha Tridacna squamosa primer), Hip_hip_YZ_R (Che oyster primer), Tri_max_YZ_R (Tridacna (chamestrachea) maxima (R ding). primer), Tri_squ_YZ_R
(squama Concha Tridacna squamosa primer), Tri_cro_YZ_R (sarranine Concha Tridacna squamosa primer).
Concha Tridacna squamosa shown in Fig. 1 by extract DNA after, enter performing PCR, result as shown in Figure of description 2, from the agar of Fig. 2
Sugared gel electrophoresis figure can be seen that there is obvious bright band in the 3rd hole, therefore may certify that this Concha Tridacna squamosa is Che oyster.
Concha Tridacna squamosa shown in Fig. 3 by extract DNA after, enter performing PCR, result as shown in Figure of description 4, from the agar of Fig. 4
Gel electrophoresis figure can be seen that there is obvious bright band in the 5th hole, therefore may certify that this Concha Tridacna squamosa is squama Concha Tridacna squamosa.
Concha Tridacna squamosa shown in Fig. 5 by extract DNA after, enter performing PCR, result as shown in Figure of description 6, from the agar of Fig. 6
Gel electrophoresis figure can be seen that there is obvious bright band in the 6th hole, therefore may certify that this Concha Tridacna squamosa is sarranine Concha Tridacna squamosa.
Therefore, using the Concha Tridacna squamosa mitochondrion 16S gene amplification primer of the present invention, can be rapid, accurately identify Concha Tridacna squamosa
Species.
Above disclosed be only presently preferred embodiments of the present invention, certainly the right of the present invention can not be limited with this
Scope, the equivalent variations therefore made according to the claims in the present invention, still fall within the scope that the present invention is covered.
Sequence table
<110>University Of Hainan
<120>Based on Concha Tridacna squamosa mitochondrion 16S gene amplification primer and its application
<130> 2016
<160> 12
<210> 1
<211>345
<212>DNA
<213>Tridacna_squamosa （Squama Concha Tridacna squamosa）
<400> 1
CCTTAAAGTA GCGTGATAAG TCGGCCTTTA ATTGGGGTCT GGTATGAAAG GGTTGACGTC 60
GGAAAAACTG TCTCGGAAGA ACTAAGTGAA ATTTACTTTT AAGTGAAAAG GCTTAAATTA 120
AAGTAAAAGA CGAGAAGACC CTGTCGAGCT TGAGGGATTT GAATGTTTAT TAAACGTGAT 180
TAGGAGTTTG GCTGGGGCAG CAATGGAGAT ACCCTCCGTT TTTAATGCAA AGATCCATTA 240
TACTTAGAAT AATGAAAAAA GAAAAAAGCT ACCGCAGGGA TAACAGCACA AGACCGCCTC 300
AGAGGTCGTA TCGAAGGCGG TAAGTGTGAC CTCGATGTTG GATTA 345
<210> 2
<211>491
<212>DNA
<213> Tridacna derasa （No squama Concha Tridacna squamosa）
<400> 2
AAAACATCTC TTCTAGAACT ATATTAGAAG TAGGCCCTGC CCGGTGCCCT GCGGGGTTAA 60
CGGCGGTGTT AAGCCTTAAA GTAGCGTGAT AAGTTGGCCT TTAATTGGGG TCTGGTATGA 120
AGGGGTTGAC GTCGGAGAAA CTGTCTCGGA AAGATAAAAT GAAATTTACG TCCTAGTGAA 180
AAGGCTGGGA TGGCTGTAAA AGACGAGAAG ACCCTGTCGA GCTTGAGGGA TATGAATGTT 240
TTAACAACAT GATTAGGAGT TTGGCTGGGG CAGCAATGGA GGTAGCCTCC ATTTTTAAAG 300
CAAAGATCCA TTAGAAAGAG ATAATGATAA AAGGAAAAAG TTACCGCAGG GATAACAGCA 360
CAAGACCGCC TCAGAGGTCG TATCGAAGGC GGTGAGTGTG ACCTCGATGT TGGATTAGGG 420
TGAACCCTTT CGTGCAGGAG CGAAAGTTGG TGGGACTGTT CTTCCTTTAA TACCCTACAT 480
GATCTGAGTT C 491
<210> 3
<211>503
<212>DNA
<213> Tridacna maxima （Tridacna (chamestrachea) maxima (R ding).）
<400> 3
CGCCTGTTTA TCAAAAACAT CTCTTCTAGC ATGATATTAG AAGTAGGCCC TGCCCGGTGC 60
CTTGCGAGGT AAACGGCGGT GTTAAACCTT AAAGTAGCGT GATAAGTCGG CCTTTAATTG 120
GGGTCTGGTA TGAAGGGGTT GACGTCGGAA AAACTGTCTC AGAAAAATTA AATGAAATTT 180
ACTTTTAAGT GAAAAGGCTT AAATGAGAGT AAAAGACGAG AAGGCCCTGT CGAGCTTGAG 240
GGATTTGAAT ATTTAGTAAA TGTGATTAGG AGTTTGGCTG GGGCAGCAAT GGAGATAGCC 300
TCCGTTTTTA AAGCAAAGAT CCATTATGTT CAAGATAATG AAAAAGGAAA AAGCTACCGC 360
AGGGATAACA GCACAAGACC GCCTCAGAGG TCGTATCGAA GGCGGTAAGT GTGACCTCGA 420
TGTTGGATTA GGGTAAACCT CTTTGTGCAG GAGCAAAGTG GGTAGGACTG TTCTTCCTTT 480
AATCCCCTAC ATGATCTGAG TTC 503
<210> 4
<211>500
<212>DNA
<213> Tridacna gigas （Ku Shi Concha Tridacna squamosa）
<400> 4
TCGCCTGTTT TTCAAAAACA TCTCTTCTAG AACTATATTA GAAGTAGGCC CTGCCCGGTG 60
CCCTGCGGGG TTAACGGCGG TGTTAAGCCT TAAAGTAGCG TGATAAGTTG GCCTTTAATT 120
GGGGTCTGGT ATGAAGGGGT TGACGTCGGA GAAACTGTCT CGGAAAGATA AAATGAAATT 180
TACGTCCTAG TGAAAAGGCT GGGATGGCTG TAAAAGACGA GAAGACCCTG TCGAGCTTGA 240
GGGATATGAA TGTTTTAACA ACATGATTAG GAGTTTGGCT GGGGCAGCAA TGGAGGTAGC 300
CTCCATTTTT AAAGCAAAGA TCCATTAGAA AGAGATAATG ATAAAAGGAA AAAGTTACCG 360
CAGGGATAAC AGCACAAGAC CGCCTCAGAG GTCGTATCGA AGGCGGTGAG TGTGACCTCG 420
ATGTTGGATT AGGGTGAACC CTTTCGTGCA GGAGCGAAAG TTGGTGGGAC TGTTCTTCCT 480
TTAATACCCT ACGTGATCTG 500
<210> 5
<211>504
<212>DNA
<213> Tridacna crocea （Sarranine Concha Tridacna squamosa）
<400> 5
TCGCCTGTTT ATCAAAAACA TCTCTTCTAG TATGATATTA GAAGTAGGCC CTGCCCGGTG 60
CCTTGCGAGG TAAACGGCGG TGTTAAACCT TAAAGTAGCG TGATAAGTCG GCCTTTAATT 120
GGGGTCTGGT ATGAAAGGGT TGACGTCGGA AAAACTGTCT CGGAAGAATT AAGTGAAATT 180
TACTTTTAAG TGAAAAGGCT TAAATTAAAG TAAAAGACGA GAAGACCCTG TCGAGCTTGA 240
GGGATTTGAA TGTTTATCAA ACGTGATTAG GAGTTTGGCT GGGGCAGCAA TGGAGGTAGC 300
CTCCGTTTTT AGCGCATAGA TCCATTTTAT CTAAGATAAT GAAAAAAGAA AAAAGCTACC 360
GCAGGGATAA CAGCACAAGA CCGCCTCAGA GGTCGTATCG AAGGCGGTAA GTGTGACCTC 420
GATGTTGGAT TAGGGTAAAC CTCTTTGTGC AGGAGCAAAG CGGGTAGGAC TGTTCTTCCT 480
TTAATCCCCT ACATGATCTG AGTT 504
<210> 6
<211>450
<212>DNA
<213> Hippopus hippopus （Che oyster）
<400> 6
GAAGTATACT GGAAGTAGGC CCTCGCCCGG TGCCCTGCGG GGTTAACGGC GGTGTTAAAC 60
CTTAAAGTAG CGTGATAGAT TGGCCTTTAA TTGGGGTCTG GTATGAATGG GTTGACGTCG 120
GAAATGCTGT CTCAAAAAAA TATAATGAAA TTTACTTTTC AGTGAAAAGG CTGAAATTTG 180
AGTAAAAGAC GAGAAGACCC TATCGAGCTT GAGGGATTCT AATGTTGTAA AACCGTTAGA 240
AGGAGTTTGG CTGGGGCAGC AACGGAGGTA ACCTCCGTTT TTAAGTCGAA GATCCATTAG 300
ATTGGTTAAT GATAAGAGGA AAAAGTTACC GTAGGGATAA CAGCACCAGA CCGCCTCAGA 360
GGCCGTATCG AAGGCGGAAG GTGTGACCTC GATGTTGGAT CACGGTGAAC CGCATGGTGC 420
AGGAGCTATG CAGGTGGGAG TGTTCTTCCT 450
<210> 7
<211>24
<212>DNA
<213>Forward primer
<400> 7
F: GGCCTTTAATTGGGGTCTGGTATG
<210> 8
<211>24
<212>DNA
<213>Squama Concha Tridacna squamosa primer
<400> 8
TCTAAGTATAATGGATCTTTGCAT
<210> 9
<211>25
<212>DNA
<213>Tridacna (chamestrachea) maxima (R ding). primer
<400> 9
CATTTACTAAATATTCAAATCCCTC
<210> 10
<211>24
<212>DNA
<213>Ku Shi Concha Tridacna squamosa primer
<400> 10
TCTCTTTCTAATGGATCTTTGCTT
<210> 11
<211>25
<212>DNA
<213>Sarranine Concha Tridacna squamosa primer
<400> 11
TCTTAGATAAAATGGATCTATGCGC
<210> 12
<211>25
<212>DNA
<213>Che oyster primer
<400> 12
CTTCTAACGGTTTTACAACATTAGA
Claims (2)
1. be based on Concha Tridacna squamosa mitochondrion 16S gene amplification primer it is characterised in that：
F:GGCCTTTAATTGGGGTCTGGTATG
Tri_squ_YZ_R squama Concha Tridacna squamosa primer:TCTAAGTATAATGGATCTTTGCAT
Tri_max_YZ_R Tridacna (chamestrachea) maxima (R ding). primer:CATTTACTAAATATTCAAATCCCTC
Tri_gig_YZ_R Ku Shi Concha Tridacna squamosa primer:TCTCTTTCTAATGGATCTTTGCTT
Tri_cro_YZ_R sarranine Concha Tridacna squamosa primer:TCTTAGATAAAATGGATCTATGCGC
Hip_hip_YZ_R Che oyster primer:CTTCTAACGGTTTTACAACATTAGA.
2. the application based on Concha Tridacna squamosa mitochondrion 16S gene amplification primer in identification Concha Tridacna squamosa species described in claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610966874.1A CN106399548A (en) | 2016-10-28 | 2016-10-28 | Tridacna mitochondrion 16S gene amplification primer and application thereof |
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|---|---|---|---|
| CN201610966874.1A CN106399548A (en) | 2016-10-28 | 2016-10-28 | Tridacna mitochondrion 16S gene amplification primer and application thereof |
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| Publication Number | Publication Date |
|---|---|
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| CN (1) | CN106399548A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108950017A (en) * | 2018-08-08 | 2018-12-07 | 中国科学院南海海洋研究所 | A kind of southern china sea area Tridacnidae species mitochondria 16S rRNA gene universal amplification primer and its screening technique |
| CN110669769A (en) * | 2019-10-18 | 2020-01-10 | 中国科学院南海海洋研究所 | Tridacna immunity-related SAA gene and application thereof in preparation of tridacna pathological detection reagent |
| CN110889844A (en) * | 2019-11-29 | 2020-03-17 | 哈尔滨工程大学 | Coral distribution and health condition assessment method based on deep clustering analysis |
| CN111763748A (en) * | 2020-07-14 | 2020-10-13 | 中国科学院南海海洋研究所 | Universal single primer, kit and identification method for identifying tridacna, tridacna without scales and trioyster |
| CN111763747A (en) * | 2020-07-14 | 2020-10-13 | 中国科学院南海海洋研究所 | Universal single primer, kit and identification method suitable for identifying common giant clam species in south sea area |
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2016
- 2016-10-28 CN CN201610966874.1A patent/CN106399548A/en active Pending
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108950017A (en) * | 2018-08-08 | 2018-12-07 | 中国科学院南海海洋研究所 | A kind of southern china sea area Tridacnidae species mitochondria 16S rRNA gene universal amplification primer and its screening technique |
| CN108950017B (en) * | 2018-08-08 | 2020-05-12 | 中国科学院南海海洋研究所 | Universal amplification primer for mitochondria 16S rRNA gene of tridacnidae species in southern China and screening method thereof |
| CN110669769A (en) * | 2019-10-18 | 2020-01-10 | 中国科学院南海海洋研究所 | Tridacna immunity-related SAA gene and application thereof in preparation of tridacna pathological detection reagent |
| CN110669769B (en) * | 2019-10-18 | 2021-06-01 | 中国科学院南海海洋研究所 | Tridacna immunity-related SAA gene and application thereof in preparation of tridacna pathological detection reagent |
| CN110889844A (en) * | 2019-11-29 | 2020-03-17 | 哈尔滨工程大学 | Coral distribution and health condition assessment method based on deep clustering analysis |
| CN110889844B (en) * | 2019-11-29 | 2023-03-21 | 哈尔滨工程大学 | Coral distribution and health condition assessment method based on deep clustering analysis |
| CN111763748A (en) * | 2020-07-14 | 2020-10-13 | 中国科学院南海海洋研究所 | Universal single primer, kit and identification method for identifying tridacna, tridacna without scales and trioyster |
| CN111763747A (en) * | 2020-07-14 | 2020-10-13 | 中国科学院南海海洋研究所 | Universal single primer, kit and identification method suitable for identifying common giant clam species in south sea area |
| CN111763747B (en) * | 2020-07-14 | 2022-04-29 | 中国科学院南海海洋研究所 | Universal single primer, kit and identification method suitable for identifying common giant clam species in south sea area |
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