CN106399389A - Method for preparing ethanol from loofah stems - Google Patents
Method for preparing ethanol from loofah stems Download PDFInfo
- Publication number
- CN106399389A CN106399389A CN201610544823.XA CN201610544823A CN106399389A CN 106399389 A CN106399389 A CN 106399389A CN 201610544823 A CN201610544823 A CN 201610544823A CN 106399389 A CN106399389 A CN 106399389A
- Authority
- CN
- China
- Prior art keywords
- ramulus luffae
- solution
- ethanol
- granule
- pretreatment
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a method for preparing ethanol from loofah stems, and belongs to the technical field of biomass energy. The ethanol is prepared by conducting such steps as drying, crushing, pre-processing, hydrolyzing and fermenting the loofah stems and the like. With the application of the method provided by the invention, the uncertainty of a microbial enzyme fermentation sugar making process can be avoided, so that technical risks are reduced; the method provided by the invention, as a green and environment-friendly process, is high in sugar conversion rate and low in the amount of generated residues; and the method provided by the invention is relatively low in hydrolysis temperature, low in energy consumption and is conducive to the popularization and application of related technologies.
Description
Technical field
The invention belongs to biomass energy technology field is and in particular to a kind of method preparing ethanol using Ramulus Luffae.
Background technology
With the growth of population, grain, energy and environment are the main challenges of facing mankind.Renewable and clean energy resource is produced with cellulosic material and is increasingly subject to extensive concern, and be considered as industry the most promising with agricultural waste material for raw material production alcohol fuel.Fructus Luffae is very wide in China's cultivated area, also produces substantial amounts of Ramulus Luffae every year while harvesting fruit.In Ramulus Luffae, protein content is low but carbohydrate content is high, and individual plants carbohydrate content is up to the 82.6% of dry, thus is the quality raw materials producing biomass energy.Both at home and abroad the research to tendril transforming fuel alcohol starts to walk, but compared with making raw material with corn straw, current alcohol yied also ratio is relatively low, bottleneck be tendril be degraded into fermentable sugars technology also undesirable.Therefore, the efficient degradation technique of invention tendril is respectively provided with high economic worth and social benefit to the comprehensive exploitation of tendril and the recovery energy of lignocellulose.
Producing bio-ethanol using lignocellulosic material has important meaning to alleviating energy crisis and ecological deterioration（Biomass Chemical Engineering, 2012,46 (3), 39-44）.But during enzyme hydrolysiss, due to portion of cellulose enzyme with hemicellulase is irreversible adsorbs the loss that can lead to enzymatic activity on substrate particularly lignin surface in the way of ineffective adsorption, the complexity of the crystalline texture of cellulose itself and hemicellulose structure is also the key factor leading to hydrolysis efficiency low in addition.
Content of the invention
The technical problem to be solved in the present invention is to provide a kind of method preparing ethanol using Ramulus Luffae, to solve Ramulus Luffae and be degraded into fermentable sugars not exclusively to lead to the low problem of alcohol getting rate.The present invention can avoid the uncertainty of microbial enzyme fermentation sugar refining technology, reduces technical risk；Present invention sugar high conversion rate, the level of residue of generation is few, is environmental type technique；The hydrolysis temperature of the present invention is relatively low, and energy resource consumption is few, is conducive to the promotion and application of correlation technique.
In order to solve above technical problem, the present invention employs the following technical solutions：
A kind of method preparing ethanol using Ramulus Luffae, comprises the following steps：
S1：Ramulus Luffae 2-3h is rinsed using saline solution, is placed on temperature to dry to water content≤12% at being 93-104 DEG C Ramulus Luffae after rinsing, prepared dry silk melon vine；
S2：The dry silk melon vine that step S1 is obtained is pulverized, and sifts out, prepared Ramulus Luffae granule；
S3：The Ramulus Luffae granule that step S2 is obtained is put in microwave reactor, add solution A mix homogeneously, it is heated to 60-65 DEG C, it is pretreatment 0.8-1.2h under 200-300r/min in speed of agitator, filter after being then cooled to room temperature, the Ramulus Luffae granule of prepared pretreatment, the natural polymers such as the cellulose of Ramulus Luffae, lignin can mutually be dissociated by pretreatment, is conducive to the hydrolysis of next step；Described solution A is butanone, ethanol, the mixed liquor of ammonium carboxylate salt, the mass concentration of described butanone is 8%-10%, the mass concentration of described ethanol is 60%-80%, and the mass concentration of described ammonium carboxylate salt is 6%-8%, and described butanone solution, ethanol solution, the volume ratio of ammonium carboxylate salts are 10-20:12-25:1-2, described Ramulus Luffae granule is 1 with the solid-liquid ratio of solution A:10-13g/mL；Ammonium carboxylate salt has potentiation with butanone, the mixed separation making lignin of ethanol；
S4：The Ramulus Luffae granule of the pretreatment that step S3 is obtained is put in ultrasound reactor, adds solution B mix homogeneously, and regulation pH is 1.3-1.7, it is heated to 71-76 DEG C, process 1.2-1.8h under speed of agitator is for 300-400r/min, filter after being then cooled to room temperature, prepared sugar liquid；Described solution B is the mixed liquor of carbon-based sodium sulfonate, sulphuric acid, and the mass concentration of described carbon-based sodium sulfonate is 5%-7%, and the mass concentration of described sulphuric acid is 4%-6%, and described carbon-based sodium sulfonate solution, the volume ratio of sulfuric acid solution are 6-10:5-8, the Ramulus Luffae granule of described pretreatment and the solid-liquid ratio of solution B are 1:4-8g/mL；
S5：The sugar liquid that step S4 is obtained accesses yeast, adds carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate, and control temperature is 26-28 DEG C of bottom fermentation, ethanol is obtained through dehydration；Described yeast quality is the 9%-11% of sugar liquid quality, and described carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality are respectively 0.18%-0.22%, 0.02%-0.04%, 0.16%-0.21%, 0.08%-0.12%, 0.14%-0.16% of sugar liquid quality.
Further, the concentration of saline solution described in step S1 is 10%-15%.
Further, the mesh number of sieve described in step S2 is 120-200.
Further, the temperature of the pretreatment in microwave reactor of Ramulus Luffae granule described in step S3 is 65 DEG C, and rotating speed is 300r/min, and the time is 0.8h.
Further, Ramulus Luffae granule described in step S3 and the solid-liquid ratio of solution A are 1:12g/mL.
Further, the pH that the Ramulus Luffae granule of pretreatment described in step S4 is processed in ultrasound reactor is 1.7, and temperature is 76 DEG C, and rotating speed is 400r/min, and the time is 1.2h.
Further, the Ramulus Luffae granule of pretreatment described in step S4 and the solid-liquid ratio of solution B are 1:6g/mL.
Further, yeast quality described in step S5 is the 10% of sugar liquid quality, and described carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality are respectively 0.2%, 0.03%, 0.2%, 0.1%, the 0.15% of sugar liquid quality.
The invention has the advantages that：
（1）The present invention prepares ethanol low cost, and raw material sources are wide；
（2）Preparation process is simple of the present invention, less investment, it is suitable for large-scale promotion and application；
（3）The uncertainty of microbial enzyme fermentation sugar refining technology can be avoided, reduce technical risk；
（4）Sugared high conversion rate, the level of residue of generation is few, is environmental type technique；
（5）Hydrolysis temperature is relatively low, and energy resource consumption is few, is conducive to the promotion and application of correlation technique；
（6）The sugared yield that the method for this is obtained is 92.15%-93.65%, and alcohol getting rate is 85.63%-86.81%, and purity of alcohol is for 99.52%-99.66% it is seen that can high-yield ethanol using the method for the present invention.
Specific embodiment
For ease of more fully understanding the present invention, it is illustrated by following examples, these embodiments belong to protection scope of the present invention, but do not limit the scope of the invention.
In an embodiment, the described method preparing ethanol using Ramulus Luffae, comprises the following steps：
S1：Using the saline solution rinsing Ramulus Luffae 2-3h for 10%-15% for the concentration, after rinsing, Ramulus Luffae is placed on temperature to dry to water content≤12% at being 93-104 DEG C, prepared dry silk melon vine；
S2：The dry silk melon vine that step S1 is obtained is pulverized, and crosses 120-200 mesh sieve, prepared Ramulus Luffae granule；
S3：The Ramulus Luffae granule that step S2 is obtained is put in microwave reactor, adds solution A mix homogeneously, is heated to 60-65 DEG C, is pretreatment 0.8-1.2h under 200-300r/min in speed of agitator, filters, the Ramulus Luffae granule of prepared pretreatment after being then cooled to room temperature；Described solution A is butanone, ethanol, the mixed liquor of ammonium carboxylate salt, the mass concentration of described butanone is 8%-10%, the mass concentration of described ethanol is 60%-80%, and the mass concentration of described ammonium carboxylate salt is 6%-8%, and described butanone solution, ethanol solution, the volume ratio of ammonium carboxylate salts are 10-20:12-25:1-2, described Ramulus Luffae granule is 1 with the solid-liquid ratio of solution A:10-13g/mL；
S4：The Ramulus Luffae granule of the pretreatment that step S3 is obtained is put in ultrasound reactor, adds solution B mix homogeneously, and regulation pH is 1.3-1.7, it is heated to 71-76 DEG C, process 1.2-1.8h under speed of agitator is for 300-400r/min, filter after being then cooled to room temperature, prepared sugar liquid；Described solution B is the mixed liquor of carbon-based sodium sulfonate, sulphuric acid, and the mass concentration of described carbon-based sodium sulfonate is 5%-7%, and the mass concentration of described sulphuric acid is 4%-6%, and described carbon-based sodium sulfonate solution, the volume ratio of sulfuric acid solution are 6-10:5-8, the Ramulus Luffae granule of described pretreatment and the solid-liquid ratio of solution B are 1:4-8g/mL；
S5：The sugar liquid that step S4 is obtained accesses yeast, adds carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate, and control temperature is 26-28 DEG C of bottom fermentation, ethanol is obtained through dehydration；Described yeast quality is the 9%-11% of sugar liquid quality, and described carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality are respectively 0.18%-0.22%, 0.02%-0.04%, 0.16%-0.21%, 0.08%-0.12%, 0.14%-0.16% of sugar liquid quality.
Below by more specific embodiment, the present invention will be described.
Embodiment 1
A kind of method preparing ethanol using Ramulus Luffae, comprises the following steps：
S1：Ramulus Luffae is placed on temperature to dry to water content≤12% at being 93-104 DEG C after rinsing, prepared dry silk melon vine by the saline solution rinsing Ramulus Luffae 2.5h being 12% using concentration；
S2：The dry silk melon vine that step S1 is obtained is pulverized, and crosses 150 mesh sieve, prepared Ramulus Luffae granule；
S3：The Ramulus Luffae granule that step S2 is obtained is put in microwave reactor, adds solution A mix homogeneously, is heated to 63 DEG C, is pretreatment 1h under 200-300r/min in speed of agitator, filters, the Ramulus Luffae granule of prepared pretreatment after being then cooled to room temperature；Described solution A is butanone, ethanol, the mixed liquor of ammonium carboxylate salt, the mass concentration of described butanone is 9%, the mass concentration of described ethanol is 70%, and the mass concentration of described ammonium carboxylate salt is 7%, and described butanone solution, ethanol solution, the volume ratio of ammonium carboxylate salts are 15:18:1, described Ramulus Luffae granule is 1 with the solid-liquid ratio of solution A:12g/mL；
S4：The Ramulus Luffae granule of the pretreatment that step S3 is obtained is put in ultrasound reactor, adds solution B mix homogeneously, and adjusting pH is 1.5, is heated to 74 DEG C, processes 1.6h under speed of agitator is for 400r/min, filters, prepared sugar liquid after being then cooled to room temperature；Described solution B is the mixed liquor of carbon-based sodium sulfonate, sulphuric acid, and the mass concentration of described carbon-based sodium sulfonate is 6%, and the mass concentration of described sulphuric acid is 5%, and described carbon-based sodium sulfonate solution, the volume ratio of sulfuric acid solution are 8:6, the Ramulus Luffae granule of described pretreatment and the solid-liquid ratio of solution B are 1:6g/mL；
S5：The sugar liquid that step S4 is obtained accesses yeast, adds carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate, and control temperature is 27 DEG C of bottom fermentations, ethanol is obtained through dehydration；Described yeast quality is the 10% of sugar liquid quality, and described carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality are respectively 0.2%, 0.03%, 0.2%, 0.1%, the 0.15% of sugar liquid quality.
Embodiment 2
A kind of method preparing ethanol using Ramulus Luffae, comprises the following steps：
S1：Ramulus Luffae is placed on temperature to dry to water content≤12% at being 93-104 DEG C after rinsing, prepared dry silk melon vine by the saline solution rinsing Ramulus Luffae 3h being 10% using concentration；
S2：The dry silk melon vine that step S1 is obtained is pulverized, and crosses 120 mesh sieve, prepared Ramulus Luffae granule；
S3：The Ramulus Luffae granule that step S2 is obtained is put in microwave reactor, adds solution A mix homogeneously, is heated to 60 DEG C, is pretreatment 1.2h under 200r/min in speed of agitator, filters, the Ramulus Luffae granule of prepared pretreatment after being then cooled to room temperature；Described solution A is butanone, ethanol, the mixed liquor of ammonium carboxylate salt, the mass concentration of described butanone is 8%, the mass concentration of described ethanol is 60%, and the mass concentration of described ammonium carboxylate salt is 6%, and described butanone solution, ethanol solution, the volume ratio of ammonium carboxylate salts are 10:12:1, described Ramulus Luffae granule is 1 with the solid-liquid ratio of solution A:10g/mL；
S4：The Ramulus Luffae granule of the pretreatment that step S3 is obtained is put in ultrasound reactor, adds solution B mix homogeneously, and adjusting pH is 1.3, is heated to 71 DEG C, processes 1.8h under speed of agitator is for 300r/min, filters, prepared sugar liquid after being then cooled to room temperature；Described solution B is the mixed liquor of carbon-based sodium sulfonate, sulphuric acid, and the mass concentration of described carbon-based sodium sulfonate is 5%, and the mass concentration of described sulphuric acid is 4%, and described carbon-based sodium sulfonate solution, the volume ratio of sulfuric acid solution are 6:5, the Ramulus Luffae granule of described pretreatment and the solid-liquid ratio of solution B are 1:4g/mL；
S5：The sugar liquid that step S4 is obtained accesses yeast, adds carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate, and control temperature is 26 DEG C of bottom fermentations, ethanol is obtained through dehydration；Described yeast quality is the 9% of sugar liquid quality, and described carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality are respectively 0.18%, 0.02%, 0.16%, 0.08%, the 0.14% of sugar liquid quality.
Embodiment 3
A kind of method preparing ethanol using Ramulus Luffae, comprises the following steps：
S1：Ramulus Luffae is placed on temperature to dry to water content≤12% at being 93-104 DEG C after rinsing, prepared dry silk melon vine by the saline solution rinsing Ramulus Luffae 2h being 15% using concentration；
S2：The dry silk melon vine that step S1 is obtained is pulverized, and crosses 200 mesh sieve, prepared Ramulus Luffae granule；
S3：The Ramulus Luffae granule that step S2 is obtained is put in microwave reactor, adds solution A mix homogeneously, is heated to 65 DEG C, is pretreatment 0.8h under 300r/min in speed of agitator, filters, the Ramulus Luffae granule of prepared pretreatment after being then cooled to room temperature；Described solution A is butanone, ethanol, the mixed liquor of ammonium carboxylate salt, the mass concentration of described butanone is 10%, the mass concentration of described ethanol is 80%, and the mass concentration of described ammonium carboxylate salt is 8%, and described butanone solution, ethanol solution, the volume ratio of ammonium carboxylate salts are 20:25:2, described Ramulus Luffae granule is 1 with the solid-liquid ratio of solution A:13g/mL；
S4：The Ramulus Luffae granule of the pretreatment that step S3 is obtained is put in ultrasound reactor, adds solution B mix homogeneously, and adjusting pH is 1.7, is heated to 76 DEG C, processes 1.2h under speed of agitator is for 400r/min, filters, prepared sugar liquid after being then cooled to room temperature；Described solution B is the mixed liquor of carbon-based sodium sulfonate, sulphuric acid, and the mass concentration of described carbon-based sodium sulfonate is 7%, and the mass concentration of described sulphuric acid is 6%, and described carbon-based sodium sulfonate solution, the volume ratio of sulfuric acid solution are 10:8, the Ramulus Luffae granule of described pretreatment and the solid-liquid ratio of solution B are 1:8g/mL；
S5：The sugar liquid that step S4 is obtained accesses yeast, adds carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate, and control temperature is 28 DEG C of bottom fermentations, ethanol is obtained through dehydration；Described yeast quality is the 11% of sugar liquid quality, and described carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality are respectively 0.22%, 0.04%, 0.21%, 0.12%, the 0.16% of sugar liquid quality.
The sugar liquid taking out appropriate embodiment 1-3 is boiled, is inactivated, and appropriateness dilution, measures fermentation sugared content with DNS colorimetric fixed sugar method, and calculates sugared yield；The method of detection embodiment 1-3 prepares alcohol getting rate and purity simultaneously, and result is as shown in the table：
| Embodiment | Sugared yield, % | Alcohol getting rate, % | Purity of alcohol, % |
| 1 | 93.49 | 86.31 | 99.59 |
| 2 | 92.15 | 85.62 | 99.52 |
| 3 | 93.65 | 86.81 | 99.66 |
As seen from the above table, by embodiment of the present invention 1-3 method be obtained sugared yield be 92.15%-93.65%, alcohol getting rate be 85.63%-86.81%, purity of alcohol be 99.52%-99.66%, illustrate sugared high conversion rate, can high-yield ethanol using the method for the present invention.
Above content it cannot be assumed that the present invention be embodied as be confined to these explanations; for general technical staff of the technical field of the invention; without departing from the inventive concept of the premise; some simple deduction or replace can also be made, all should be considered as belonging to the scope of patent protection that the present invention is determined by the claims submitted to.
Claims (8)
1. a kind of prepare the method for ethanol it is characterised in that comprising the following steps using Ramulus Luffae：
S1：Ramulus Luffae 2-3h is rinsed using saline solution, is placed on temperature to dry to water content≤12% at being 93-104 DEG C Ramulus Luffae after rinsing, prepared dry silk melon vine；
S2：The dry silk melon vine that step S1 is obtained is pulverized, and sifts out, prepared Ramulus Luffae granule；
S3：The Ramulus Luffae granule that step S2 is obtained is put in microwave reactor, adds solution A mix homogeneously, is heated to 60-65 DEG C, is pretreatment 0.8-1.2h under 200-300r/min in speed of agitator, filters, the Ramulus Luffae granule of prepared pretreatment after being then cooled to room temperature；Described solution A is butanone, ethanol, the mixed liquor of ammonium carboxylate salt, the mass concentration of described butanone is 8%-10%, the mass concentration of described ethanol is 60%-80%, and the mass concentration of described ammonium carboxylate salt is 6%-8%, and described butanone solution, ethanol solution, the volume ratio of ammonium carboxylate salts are 10-20:12-25:1-2, described Ramulus Luffae granule is 1 with the solid-liquid ratio of solution A:10-13g/mL；
S4：The Ramulus Luffae granule of the pretreatment that step S3 is obtained is put in ultrasound reactor, adds solution B mix homogeneously, and regulation pH is 1.3-1.7, it is heated to 71-76 DEG C, process 1.2-1.8h under speed of agitator is for 300-400r/min, filter after being then cooled to room temperature, prepared sugar liquid；Described solution B is the mixed liquor of carbon-based sodium sulfonate, sulphuric acid, and the mass concentration of described carbon-based sodium sulfonate is 5%-7%, and the mass concentration of described sulphuric acid is 4%-6%, and described carbon-based sodium sulfonate solution, the volume ratio of sulfuric acid solution are 6-10:5-8, the Ramulus Luffae granule of described pretreatment and the solid-liquid ratio of solution B are 1:4-8g/mL；
S5：The sugar liquid that step S4 is obtained accesses yeast, adds carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate, and control temperature is 26-28 DEG C of bottom fermentation, ethanol is obtained through dehydration；Described yeast quality is the 9%-11% of sugar liquid quality, and described carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality are respectively 0.18%-0.22%, 0.02%-0.04%, 0.16%-0.21%, 0.08%-0.12%, 0.14%-0.16% of sugar liquid quality.
2. according to claim 1 using Ramulus Luffae prepare ethanol method it is characterised in that saline solution described in step S1 concentration be 10%-15%.
3. according to claim 1 using Ramulus Luffae prepare ethanol method it is characterised in that sieve described in step S2 mesh number be 120-200.
4. according to claim 1 using Ramulus Luffae prepare ethanol method it is characterised in that the pretreatment in microwave reactor of Ramulus Luffae granule described in step S3 temperature be 65 DEG C, rotating speed be 300r/min, the time be 0.8h.
5. according to claim 1 prepare the method for ethanol it is characterised in that the solid-liquid ratio of Ramulus Luffae granule described in step S3 and solution A is 1 using Ramulus Luffae:12g/mL.
6. according to claim 1 prepare the method for ethanol it is characterised in that the pH that the Ramulus Luffae granule of pretreatment described in step S4 is processed in ultrasound reactor is 1.7 using Ramulus Luffae, temperature is 76 DEG C, and rotating speed is 400r/min, and the time is 1.2h.
7. according to claim 1 prepare the method for ethanol it is characterised in that the solid-liquid ratio of Ramulus Luffae granule and solution B of pretreatment described in step S4 is 1 using Ramulus Luffae:6g/mL.
8. the method preparing ethanol using Ramulus Luffae according to claim 1, it is characterized in that, yeast quality described in step S5 is the 10% of sugar liquid quality, and described carbamide, Semen phaseoli radiati powder, sodium dihydrogen phosphate, magnesium nitrate, calcium sulfate quality are respectively 0.2%, 0.03%, 0.2%, 0.1%, the 0.15% of sugar liquid quality.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610544823.XA CN106399389A (en) | 2016-07-12 | 2016-07-12 | Method for preparing ethanol from loofah stems |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610544823.XA CN106399389A (en) | 2016-07-12 | 2016-07-12 | Method for preparing ethanol from loofah stems |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106399389A true CN106399389A (en) | 2017-02-15 |
Family
ID=58006875
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610544823.XA Pending CN106399389A (en) | 2016-07-12 | 2016-07-12 | Method for preparing ethanol from loofah stems |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106399389A (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102352381A (en) * | 2011-10-08 | 2012-02-15 | 山东龙力生物科技股份有限公司 | Method using xylose production waste liquid to produce acetone and butanol |
-
2016
- 2016-07-12 CN CN201610544823.XA patent/CN106399389A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102352381A (en) * | 2011-10-08 | 2012-02-15 | 山东龙力生物科技股份有限公司 | Method using xylose production waste liquid to produce acetone and butanol |
Non-Patent Citations (1)
| Title |
|---|
| 刘荣厚等: "《生物质生物转换技术》", 31 December 2015, 上海交通大学出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104774876B (en) | A kind of method of lignocellulose biomass comprehensive utilization | |
| CN102277391B (en) | Comprehensive utilization method of straw raw materials | |
| CN101514349B (en) | Method for preparing fuel ethanol from bamboo fibers | |
| CN101696427B (en) | Method for producing fuel ethanol and 2,3-butanediol by using fibrous matter | |
| CN104805137A (en) | Method for producing gluconic acid through bioconversion of lignocellulose | |
| CN104862344A (en) | Method for producing cellulosic ethanol by fermenting agricultural and forest biomass waste thick mash | |
| CN104846034A (en) | Method for improving yield of bioethanol prepared by recycling and reusing cellulose | |
| CN103509828B (en) | Method for preparing ethanol with manioc wastes as raw materials through synergic saccharification fermentation | |
| CN106636224A (en) | Process for producing ethanol through fermentation of maize straws | |
| CN103805673B (en) | A kind of method utilizing transgenic yeast mixed fermentation to produce straw ethanol | |
| CN107502636B (en) | Method for pretreating hybrid pennisetum alopecuroides at low temperature by using ammonia water | |
| CN102517355A (en) | Method for jointly degrading lignocellulose | |
| CN105950670A (en) | Method for preparing ethyl alcohol from schisandra chinensis stems | |
| CN106191134A (en) | A kind of method utilizing Hami melon vine to prepare ethanol | |
| CN106119299A (en) | A kind of method utilizing vine to prepare ethanol | |
| CN106399389A (en) | Method for preparing ethanol from loofah stems | |
| CN106399390A (en) | Method for preparing ethanol from luffa cylindrica stems | |
| CN106591381B (en) | Method for preparing bioethanol by continuously pretreating kelp residues | |
| CN105936918A (en) | Method of preparing ethanol from muskmelon vine | |
| CN105950669A (en) | Method for preparing ethyl alcohol from Chinese gooseberry stems | |
| CN105936920A (en) | Method using broad bean straws to prepare butanol | |
| CN105925624A (en) | Method for preparing butyl alcohol by using green soy bean straws | |
| CN104278065A (en) | Method for improving yield of enzymatic hydrolysis of plant fiber materials | |
| CN105936921A (en) | Method of preparing butanol from corn straws | |
| CN106399395A (en) | Method for preparing butanol from rape stalks |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170215 |