CN106390197A - Stress active tissue engineering scaffold material and preparation method thereof - Google Patents
Stress active tissue engineering scaffold material and preparation method thereof Download PDFInfo
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- CN106390197A CN106390197A CN201610945937.5A CN201610945937A CN106390197A CN 106390197 A CN106390197 A CN 106390197A CN 201610945937 A CN201610945937 A CN 201610945937A CN 106390197 A CN106390197 A CN 106390197A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/56—Porous materials, e.g. foams or sponges
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Abstract
The invention relates to a stress active tissue engineering scaffold material prepared from polyvinylidene fluoride nanofiber, silk fibroin, polyvinyl alcohol, and a preparation method thereof, and belongs to the field of a biomedical material. In the stress active tissue engineering scaffold material, the mass ratio of the silk fibroin to the polyvinyl alcohol is 1/9 to 1/1; the mass ratio of the polyvinylidene fluoride nanofiber to the polyvinyl alcohol is 1/99 to 5/95. The preparation method is characterized in that polyvinylidene fluoride nanofiber suspension, silk fibroin solution or silk fibroin powder dispersion liquid and polyvinyl alcohol solution are mixed to obtain uniform and stable mixed suspension; casting mold forming, cryocoagulation, demoulding and freeze drying are performed to obtain cavernous bodies; then, gaseous esterification treatment is performed to prepare the stress active tissue engineering scaffold material. The stress active tissue engineering scaffold material has uniform and stable micro-structures and good biocompatibility; the substrate conditions of the biological tissue growth are provided; the piezoelectric stress response characteristics of the material suffering from external force are realized.
Description
Technical field
The present invention relates to one kind stress active mass's engineering scaffold material and preparation method thereof, belong to bio-medical material neck
Domain.
Background technology
Organizational project is the emerging interdisciplinary field growing up in the last thirty years, and wherein most basic thinking is in body
Outer separation, cultured cells are inoculated on the support with certain space structure, by mutually sticking, growing increasing between cell
Grow, extracellular matrix secretion, thus formed that there is tissue and the organ of certain 26S Proteasome Structure and Function, the support of this certain space structure
It is referred to as tissue engineering bracket, such as skin, bone, cartilage, tendon, ligament etc..Need to meet four bars as tissue engineering bracket
Part, the biocompatible materials of suitable cell tactophily, the pore structure providing cell growth and cambium are adapted
Degradation characteristic, the structure meeting tissue engineering bracket and mechanical property.From timbering material tissue repair or reconstruction process mesh
Predecessor is confined to occupancy and substitutes repair or based on occupancy rebuilds, be tissue growth provide physical space and matrix environment,
Respective reaction can not be made with physiological stress, electromagnetic action or biochemical stimulation as biological tissue, not possess biological anti-
Ying Xing, and biological respinse to the neoblastic speed of growth, the foundation of biologically active and with the healing of matrix etc. have important
Meaning.Therefore, exploitation has bionical physiological stress response, the biological reactivity material of galvanomagnetic-effect becomes current tissue and repaiies
The Main Trends of The Development of multiple material.
In human body, many tissues are natural piezoelectrics, such as：Skin, bone, cartilage, tendon, ligament etc., piezo-electric effect comes from
Collagen component in tissue, by completing the mutual conversion of mechanical energy and electric energy, the normal physiological activity that can maintain tissue is with newly
Old metabolism, is conducive to the moulding, reconstruction of tissue and function to maintain, tissue can promotion organization because of the polarization charge that piezo-electric effect produces
Growth and healing.This piezo-electric effect for biology angle be exactly biological stress activity.Piezoelectricity due to tissue
I.e. stress key effect in tissue reconstruction and repair process for the activity characteristic, will be tissue engineering bracket material design
Provide a kind of new thinking with building.
Kynoar is that have one of macromolecular material of best piezoelectric property, be provided simultaneously with good pliability, can
Processability and biocompatibility, the Kynoar with piezoelectric property is compound in existing tissue engineering material becomes tax
Give the effective ways of tissue engineering bracket material piezoelectric property.But Kynoar is according to the process of its processing conditions and process
Difference can obtain α, beta, gamma, and five kinds of crystalline phases of δ, ε can also be in the process kind phase of different processing conditions and process between five kinds of crystalline phases
Mutually convert, different crystalline phases has different characteristics, and only β crystalline phase has piezoelectric property, and Kynoar is only in high pressure
Just can obtain under conditions of electric field polarization or mechanical commutation draft.Find a kind of Kynoar that can obtain β crystalline phase, can make again
The Kynoar of β crystalline phase is dispersed in tissue engineering bracket material and obtains one kind have stress active mass's engineering rack
Material and preparation method thereof, becomes the starting point of the present invention.
Content of the invention
For above-mentioned problem, the invention aims to solving lacking of existing tissue engineering bracket material presence
Fall into, provide a kind of have stress activity tissue engineering bracket material and preparation method thereof.Realization the technical scheme is that：
One kind stress active mass's engineering scaffold material, described stress active mass's engineering scaffold material be by polyvinylidene fluoride
The cavernous body of alkene nanofiber, fibroin albumen and polyvinyl alcohol composition, stress the living of the cavernous structure obtaining through esterification treatment
Property tissue engineering bracket material, wherein fibroin albumen and polyvinyl alcohol mass ratio is 1/9～1/1, polyvinylidene fluoride nanometer fiber
Mass ratio with polyvinyl alcohol is 1/99～5/95.
Described polyvinylidene fluoride nanometer fiber is diameter 50nm-1000nm, and length is 5000nm-100000nm.
Described fibroin albumen is less than the powder shaped fibroin albumen of 500nm for pulverizing the diameter obtaining after natural silk degumming, or
After natural silk degumming, through dissolving, dialysis, the fibroin albumen that freeze-drying obtains.
A kind of stress active mass's engineering scaffold material preparation method, including the system of polyvinylidene fluoride nanometer tunica fibrosa
The preparation of standby, silk fibroin protein solution or fibroin albumen powder dispersion and poly-vinyl alcohol solution is it is characterised in that described preparation side
Method is carried out according to the following steps：
A. the polyvinylidene fluoride nanometer tunica fibrosa taking a diameter of 50nm-1000nm cuts, pulverize ultrasonic disperse in water after
The diameter 50nm-1000nm obtaining, length is the mass concentration of 5000nm-100000nm is 5wt%～20wt% polyvinylidene fluoride
Alkene nanofiber dispersion liquid.
B. the polyvinylidene fluoride nanometer fiber dispersion having configured, silk fibroin protein solution or fibroin albumen powder dispersion are taken
It is mixed to get the mass ratio of uniform and stable mixing suspension, wherein Kynoar and polyvinyl alcohol with poly-vinyl alcohol solution phase
For 1/99～5/95, fibroin albumen is 5/95～50/50 with the mass ratio of polyvinyl alcohol, Kynoar, fibroin albumen with poly-
The total soluble matters mass concentration of vinyl alcohol is 5wt%～20wt%.
C. by the uniform and stable mixing suspension of step b preparation in casting die, cryocoagulation, the demoulding, freezing is dry
Dry, obtain the cavernous body being made up of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol.
D. the cavernous body vapour phase esterification of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol composition is processed
The cavernous body being made up of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol that step c is obtained is in 80
DEG C, saturation maleic acid anhydride vapours, percent by volume 1%-5% are esterification and crosslinking 2-4 hour in the sealing atmosphere of HCl steam
Afterwards, take out cavernous body and be heat-treated 15-30min in 120 DEG C of baking ovens, obtain by polyvinylidene fluoride nanometer fiber, fibroin albumen and
Polyvinyl alcohol composition cavernous structure stress active mass's engineering scaffold material.
Due to using above technical scheme, the present invention stress active mass's engineering scaffold material can be by people after implantation human body
The compression that body motion produces to tissue engineering bracket material, the physical stimulation such as drawing-off are converted to polarization charge, pole by piezo-electric effect
Change electric charge by promotion organization growth and the healing and matrix between, realize using physical stimulation come the reconstruction of promotion organization,
Accelerate rehabilitation course.The even porous cavernous body of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol has good
Biocompatibility and uniform pore structure are that histiocytic tactophily provides good matrix condition.The present invention stress
Active mass's engineering material is polyvinylidene fluoride nanometer fiber dispersion with water as solvent, silk fibroin protein solution or fibroin albumen
Powder dispersion and poly-vinyl alcohol solution mixing, eliminating in existing portion of tissue engineering material preparation process needs organic solvent
Dissolving, because organic solvent cannot completely remove, the organic solvent of remaining is to have stronger toxicity to cell culture, breeding, makes
Become the later death of cell culture, breeding serious.The preparation not needing the tissue engineering material of organic solvent further increases
The biocompatibility of tissue engineering material.The present invention stress active mass's engineering material with polyvinylidene fluoride nanometer fiber, fibroin
Albumen and polyvinyl alcohol are material, and wherein polyvinylidene fluoride nanometer fiber has good piezo-electric effect and biocompatibility, gathers
Vinyl alcohol is a kind of synthetic material of good biocompatibility, and natural material fibroin albumen improves further and ensure that group weaver
The biocompatibility of engineering support material, particularly fibroin powder remain the cytocerastic matrix condition of natural biologic material, and three
Compound and technique the control of kind material becomes has tissue engineering bracket material that stress be active.
The preparation method of the tissue engineering bracket of the present invention, due to being with polyvinylidene fluoride nanometer fiber dispersion, fibroin
Protein solution or fibroin albumen powder dispersion are mixed with poly-vinyl alcohol solution, through casting die, cryocoagulation, the demoulding, freezing
The even porous cavernous body shape material being dried to obtain is in that context it may be convenient to adjust organizational project material by changing concentration in mixture
The size of material mesopore.The mandruka body of gained is processed through the vapour phase esterification of maleic anhydride again, and making stress activearm
Knit the fibroin albumen in engineering material or silk fibroin powder bluk recombination polyvinyl alcohol does not dissolve in water, with vapour phase esterification process time
Prolongation not only can make fibroin albumen or silk fibroin powder bluk recombination polyvinyl alcohol not dissolve in the pore structure that water keeps stable, can
The degradation speed of regulation and control fibroin albumen or silk fibroin powder bluk recombination polyvinyl alcohol further.The present invention's stress activearm weaver
Engineering support material preparation method is simple and convenient to operate, with low cost, has good controllability to preparation process, should activate
Property tissue engineering bracket material go for processing the tissue engineering bracket of Various Tissues and various shapes.
Brief description
Fig. 1 is tissue engineering bracket material surface topography electron microscope.
Specific embodiment
With reference to the accompanying drawings and examples the present invention is described in further detail.
See accompanying drawing.
One kind stress active mass's engineering scaffold material, described tissue engineering bracket material is fine by polyvinylidene fluoride nanometer
Dimension, fibroin albumen and polyvinyl alcohol composition cavernous body, the cavernous structure obtaining through esterification treatment stress activearm weaver
Engineering support material, wherein fibroin albumen and polyvinyl alcohol mass ratio are 1/9～1/1, polyvinylidene fluoride nanometer fiber and polyethylene
The mass ratio of alcohol is 1/99～5/95.
Described polyvinylidene fluoride nanometer fiber is diameter 50nm-1000nm, and length is 5000nm-100000nm.
Described fibroin albumen is less than the powder shaped fibroin albumen of 500nm for pulverizing the diameter obtaining after natural silk degumming, or
After natural silk degumming, through dissolving, dialysis, the fibroin albumen that freeze-drying obtains.
A kind of stress active mass's engineering scaffold material preparation method, including the system of polyvinylidene fluoride nanometer tunica fibrosa
The preparation of standby, silk fibroin protein solution or fibroin albumen powder dispersion and poly-vinyl alcohol solution is it is characterised in that described preparation side
Method is carried out according to the following steps：
A. the polyvinylidene fluoride nanometer tunica fibrosa taking a diameter of 50nm-1000nm cuts, pulverize ultrasonic disperse in water after
The diameter 50nm-1000nm obtaining, length is the mass concentration of 5000nm-100000nm is 5wt%～20wt% polyvinylidene fluoride
Alkene nanofiber dispersion liquid.
B. the polyvinylidene fluoride nanometer fiber dispersion having configured, silk fibroin protein solution or fibroin albumen powder dispersion are taken
It is mixed to get the mass ratio of uniform and stable mixing suspension, wherein Kynoar and polyvinyl alcohol with poly-vinyl alcohol solution phase
For 1/99～5/95, fibroin albumen is 5/95～50/50 with the mass ratio of polyvinyl alcohol, Kynoar, fibroin albumen with poly-
The total soluble matters mass concentration of vinyl alcohol is 5wt%～20wt%.
C. by the uniform and stable mixing suspension of step b preparation in casting die, cryocoagulation, the demoulding, freezing is dry
Dry, obtain the cavernous body being made up of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol.
D. the cavernous body vapour phase esterification of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol composition is processed
The cavernous body being made up of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol that step c is obtained is in 80
DEG C, saturation maleic acid anhydride vapours, percent by volume 1%-5% are esterification and crosslinking 2-4 hour in the sealing atmosphere of HCl steam
Afterwards, take out cavernous body and be heat-treated 15-30min in 120 DEG C of baking ovens, obtain by polyvinylidene fluoride nanometer fiber, fibroin albumen and
Polyvinyl alcohol composition cavernous structure stress active mass's engineering scaffold material.
Silk fibroin protein solution or fibroin albumen powder dispersion in preparation process b as above, after being natural silk degumming, warp
Cross dissolving, dialysis, the fibroin albumen that freeze-drying obtains is dispensed in water, and magnetic stirring obtains stable fibroin until being completely dissolved
Pulverize the powder shaped fibroin albumen that the diameter obtaining is less than 500nm after protein solution, or natural silk degumming to be dispersed in water, ultrasonic point
Dissipate the dispersion liquid obtaining fibroin powder, the mass concentration of silk fibroin protein solution is 5wt%～20wt%；The fibroin albumen of dispersion liquid
The mass concentration of powder is 5wt%～20wt%；Prepare during polyvinyl alcohol (PVA) solution with water as solvent, and magnetic at 80 DEG C
Stirring.
Fibroin albumen powder for mulberry silk or can squeeze after natural silk degumming, is prepared by the method for mechanical crushing, silk
The micro-structural of fibroin powder is not changed with matrix feature；Fibroin albumen for mulberry silk or can squeeze after natural silk degumming, passes through
Dissolving, dialysis, freeze-drying obtains.
With reference to embodiment, the invention will be further elaborated：
Embodiment one
A. the polyvinylidene fluoride nanometer tunica fibrosa taking a diameter of 50nm cuts, pulverize ultrasonic disperse in water after obtain straight
Footpath 50nm, length is the mass concentration of 5000nm-100000nm is 5wt% polyvinylidene fluoride nanometer fiber dispersion；
B. the polyvinylidene fluoride nanometer fiber dispersion for 5wt% by mass concentration, mass concentration is the fibroin egg of 5wt%
White water solution and the polyvinyl alcohol water solution phase that mass concentration is 5wt% are mixed to get uniform and stable mixing suspension, wherein
Kynoar is 1/99 with the mass ratio of polyvinyl alcohol, and fibroin albumen is 5/95 with the mass ratio of polyvinyl alcohol, polyvinylidene fluoride
The total soluble matters mass concentration of alkene, fibroin albumen and polyvinyl alcohol is 5wt%.
C. by the uniform and stable mixing suspension of step b preparation in casting die, cryocoagulation, the demoulding, freezing is dry
Dry, obtain the cavernous body being made up of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol.
D. the cavernous body vapour phase esterification of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol composition is processed
The cavernous body being made up of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol that step c is obtained is in 80
DEG C, saturation maleic acid anhydride vapours, percent by volume 1%, take after 2 hours for esterification and crosslinking in the sealing atmosphere of HCl steam
Go out cavernous body and be heat-treated 15min in 120 DEG C of baking ovens, obtain by polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol
Composition cavernous structure stress active mass's engineering scaffold material.
Embodiment two
A. the polyvinylidene fluoride nanometer tunica fibrosa taking a diameter of 500nm cuts, pulverize ultrasonic disperse in water after obtain
Diameter 500nm, length is the mass concentration of 50000nm is 10wt% polyvinylidene fluoride nanometer fiber dispersion.
B. the polyvinylidene fluoride nanometer fiber dispersion for 10wt% by mass concentration, mass concentration is the fibroin of 10wt%
Protein solution and the polyvinyl alcohol water solution phase that mass concentration is 10wt% are mixed to get uniform and stable mixing suspension, its
Middle Kynoar is 3/97 with the mass ratio of polyvinyl alcohol, and fibroin albumen is 20/80 with the mass ratio of polyvinyl alcohol, gathers inclined fluorine
The total soluble matters mass concentration of ethene, fibroin albumen and polyvinyl alcohol is 10wt%.
C. by the uniform and stable mixing suspension of step b preparation in casting die, cryocoagulation, the demoulding, freezing is dry
Dry, obtain the cavernous body being made up of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol.
D. the cavernous body vapour phase esterification of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol composition is processed
The cavernous body being made up of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol that step c is obtained is in 80
DEG C, saturation maleic acid anhydride vapours, percent by volume 3%, take after 3 hours for esterification and crosslinking in the sealing atmosphere of HCl steam
Go out cavernous body and be heat-treated 20min in 120 DEG C of baking ovens, obtain by polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol
Composition cavernous structure stress active mass's engineering scaffold material.
Embodiment three
A. the polyvinylidene fluoride nanometer tunica fibrosa taking a diameter of 1000nm cuts, pulverize ultrasonic disperse in water after obtain
Diameter 1000nm, length is the mass concentration of 100000nm is 20wt% polyvinylidene fluoride nanometer fiber dispersion.
B. the polyvinylidene fluoride nanometer fiber dispersion for 20wt% by mass concentration, mass concentration is the fibroin of 15wt%
Protein powder dispersion liquid and the polyvinyl alcohol water solution phase that mass concentration is 20wt% are mixed to get uniform and stable mix suspending
Liquid, wherein Kynoar are 5/95 with the mass ratio of polyvinyl alcohol, and fibroin albumen is 50/50 with the mass ratio of polyvinyl alcohol,
The total soluble matters mass concentration of Kynoar, fibroin albumen and polyvinyl alcohol is 20wt%.
C. by the uniform and stable mixing suspension of step b preparation in casting die, cryocoagulation, the demoulding, freezing is dry
Dry, obtain the cavernous body being made up of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol.
D. the cavernous body vapour phase esterification of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol composition is processed
The cavernous body being made up of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol that step c is obtained is in 80
DEG C, saturation maleic acid anhydride vapours, percent by volume 5%, take after 4 hours for esterification and crosslinking in the sealing atmosphere of HCl steam
Go out cavernous body and be heat-treated 30min in 120 DEG C of baking ovens, obtain by polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol
Composition cavernous structure stress active mass's engineering scaffold material.
Example IV
A. the polyvinylidene fluoride nanometer tunica fibrosa taking a diameter of 800nm cuts, pulverize ultrasonic disperse in water after obtain
Diameter 800nm, length is the mass concentration of 80000nm is 15wt% polyvinylidene fluoride nanometer fiber dispersion.
B. the polyvinylidene fluoride nanometer fiber dispersion for 15wt% by mass concentration, mass concentration is the fibroin of 20wt%
Protein powder dispersion liquid and the polyvinyl alcohol water solution phase that mass concentration is 5wt% are mixed to get uniform and stable mix suspending
Liquid, wherein Kynoar are 5/95 with the mass ratio of polyvinyl alcohol, and fibroin albumen is 1/1 with the mass ratio of polyvinyl alcohol, gathers
The total soluble matters mass concentration of vinylidene, fibroin albumen and polyvinyl alcohol is 5wt%.
C. by the uniform and stable mixing suspension of step b preparation in casting die, cryocoagulation, the demoulding, freezing is dry
Dry, obtain the cavernous body being made up of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol.
D. the cavernous body vapour phase esterification of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol composition is processed
The cavernous body being made up of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol that step c is obtained is in 80
DEG C, saturation maleic acid anhydride vapours, percent by volume 4%, take after 3 hours for esterification and crosslinking in the sealing atmosphere of HCl steam
Go out cavernous body and be heat-treated 25min in 120 DEG C of baking ovens, obtain by polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol
Composition cavernous structure stress active mass's engineering scaffold material.
Claims (4)
1. one kind stress active mass's engineering scaffold material it is characterised in that：Described engineering scaffold material is by polyvinylidene fluoride
The cavernous body of alkene nanofiber, fibroin albumen and polyvinyl alcohol composition, stress the living of the cavernous structure obtaining through esterification treatment
Property tissue engineering bracket material, wherein fibroin albumen and polyvinyl alcohol mass ratio is 1/9～1/1, polyvinylidene fluoride nanometer fiber
Mass ratio with polyvinyl alcohol is 1/99～5/95.
2. one kind according to claim 1 stress active mass's engineering scaffold material it is characterised in that：Described poly- inclined fluorine
Ethene nanofiber is diameter 50nm-1000nm, and length is 5000nm-100000nm.
3. according to claim 1 a kind of stress active mass's engineering scaffold material it is characterised in that described fibroin egg
It is less than the powder shaped fibroin albumen of 500nm in vain for pulverizing the diameter obtaining after natural silk degumming, or after natural silk degumming, through dissolving,
Dialysis, the fibroin albumen that freeze-drying obtains.
4. a kind of stress active mass's engineering scaffold material preparation method, including a diameter of 50nm-1000nm Kynoar
The preparation of the preparation of nano fibrous membrane, silk fibroin protein solution or fibroin albumen powder dispersion and poly-vinyl alcohol solution, its feature
It is, described preparation method is carried out according to the following steps：
A. the polyvinylidene fluoride nanometer tunica fibrosa taking a diameter of 50nm-1000nm cuts, pulverize and obtain after ultrasonic disperse in water
Diameter 50nm-1000nm, length be 5000nm-100000nm mass concentration receive for 5wt%～20wt% Kynoar
Rice fiber dispersion；
B. take the polyvinylidene fluoride nanometer fiber dispersion having configured, silk fibroin protein solution or fibroin albumen powder dispersion and gather
Glycohol solution phase is mixed to get uniform and stable mixing suspension, and wherein Kynoar and the mass ratio of polyvinyl alcohol are 1/
99～5/95, fibroin albumen is 5/95～50/50 with the mass ratio of polyvinyl alcohol, Kynoar, fibroin albumen and polyethylene
The total soluble matters mass concentration of alcohol is 5wt%～20wt%；
C. by the uniform and stable mixing suspension of step b preparation in casting die, cryocoagulation, the demoulding, freeze-drying, obtain
To the cavernous body being made up of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol；
D. the cavernous body vapour phase esterification of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol composition is processed
The cavernous body being made up of polyvinylidene fluoride nanometer fiber, fibroin albumen and polyvinyl alcohol that step c is obtained, in 80 DEG C, is satisfied
After esterification and crosslinking 2-4 hour in the sealing atmosphere being HCl steam with maleic acid anhydride vapours, percent by volume 1%-5%, take
Go out cavernous body and be heat-treated 15-30min in 120 DEG C of baking ovens, obtain by polyvinylidene fluoride nanometer fiber, fibroin albumen and polyethylene
Alcohol composition cavernous structure stress active mass's engineering scaffold material.
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| CN107158475A (en) * | 2017-06-02 | 2017-09-15 | 人福医药集团医疗用品有限公司 | One kind stress activated fibre film and preparation method thereof |
| CN110694115A (en) * | 2019-10-22 | 2020-01-17 | 上海交通大学医学院附属第九人民医院 | Method for constructing tendon tissue in vitro, and biological material and application thereof |
| CN113730662A (en) * | 2021-08-02 | 2021-12-03 | 华中科技大学 | Nanofiber 3D porous aerogel and preparation method and application thereof |
| CN113730662B (en) * | 2021-08-02 | 2023-01-03 | 华中科技大学 | Nanofiber 3D porous aerogel and preparation method and application thereof |
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