CN106386779A - Oriental mosquito fixed-point partition release method carrying Wolbachia - Google Patents
Oriental mosquito fixed-point partition release method carrying Wolbachia Download PDFInfo
- Publication number
- CN106386779A CN106386779A CN201610768629.XA CN201610768629A CN106386779A CN 106386779 A CN106386779 A CN 106386779A CN 201610768629 A CN201610768629 A CN 201610768629A CN 106386779 A CN106386779 A CN 106386779A
- Authority
- CN
- China
- Prior art keywords
- mosquito
- wolbachia
- release
- releasing
- culex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 241000255925 Diptera Species 0.000 title claims abstract description 68
- 241000604961 Wolbachia Species 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 12
- 238000005192 partition Methods 0.000 title abstract 2
- 241000256054 Culex <genus> Species 0.000 claims abstract description 30
- 241000256173 Aedes albopictus Species 0.000 claims abstract description 20
- 238000009395 breeding Methods 0.000 claims abstract description 8
- 230000001488 breeding effect Effects 0.000 claims abstract description 8
- 238000001514 detection method Methods 0.000 claims abstract description 7
- 238000004140 cleaning Methods 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 235000013601 eggs Nutrition 0.000 claims description 5
- 241000256113 Culicidae Species 0.000 claims 1
- 238000003825 pressing Methods 0.000 description 7
- 208000001490 Dengue Diseases 0.000 description 2
- 206010012310 Dengue fever Diseases 0.000 description 2
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 208000025729 dengue disease Diseases 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 241000256118 Aedes aegypti Species 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 241000521257 Hydrops Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 206010065954 Stubbornness Diseases 0.000 description 1
- 230000027326 copulation Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M99/00—Subject matter not provided for in other groups of this subclass
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Insects & Arthropods (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for releasing aedes albopictus fixed-point partitions carrying wolbachia. In the release subarea, according to different terrains and surrounding environment conditions, selecting breeding places and habitats of mosquitoes as release points, wherein the habitats are places with moist environment, light shading and luxuriant vegetation; cleaning containers filled with water and possibly spawned by mosquitoes in the release subareas; the releasing point traps aedes albopictus with a BG-Trap adult mosquito trapping device, male mosquitoes trapped by the BG-Trap adult mosquito trapping device are counted, PCR detection is carried out on the male mosquitoes to judge whether the male mosquitoes carry culex wolbachia or not, the number of positive male mosquitoes carrying the culex wolbachia and the number of negative male mosquitoes not carrying the culex wolbachia are counted respectively to obtain a releasing ratio, and then the positive male mosquitoes carrying the culex wolbachia are released at the releasing point according to the releasing ratio more than or equal to 5.
Description
Technical field
The invention belongs to mosquito control field is and in particular to a kind of aedes albopictus carrying Wolbachia pinpoints subregion
Method for releasing.
Background technology：
In fruit bat, yellow-fever mosquito and culex body extract Wolbachia and be successfully conducted into the white mosquito of Dengue vector she
In mosquito body, establish the stable mosquito strain carrying new Wolbachia.Carry male mosquito and the non-portable of Wolbachia
The ovum that Wolbachia female mosquito copulation is produced can not be developed.Carry the male mosquito of Wolbachia by a large amount of releases, permissible
Make mosquito populations quantity be reduced to be not enough to cause dengue prevalence.
Then the release area in view of the aedes albopictus carrying Wolbachia is very big, and the landform on release ground is again very multiple
Miscellaneous.How to discharge and burst size could can be made few, the method for releasing that again can reach good effect is badly in need of considering.
The method that abroad useful car discharges when walking at present to cover entire surface, very flat in the hope of reaching each area distribution
All, but this method for releasing has individual shortcoming it is simply that the possible breeding place of mosquito and possible habitat may relative quantity compare
Few, rather than the evil Radix Rehmanniae and non-habitat may be relatively many, thus can affect pressing result.
Content of the invention：
It is an object of the invention to provide one kind can adjust in time according to field mosquito quantity, sensitivity is higher and maximum limit
Degree reduces mosquito loss, and carries Wo Erbakeshi what the more place of mosquito distribution also can reach good pressing result
The aedes albopictus fixed point subregion method for releasing of body.
The present invention carry Wolbachia aedes albopictus fixed point subregion method for releasing it is characterised in that include with
Lower step：
A, prospecting landform：In release subregion, according to the ambient conditions of different terrain and surrounding, select multiplying of mosquito
Ground and
Nearby as point of release, described habitat is humid environment to habitat, shading and the luxuriant place of vegetation；
B, cleaning breeding place：It is filled with water and container that mosquito may lay eggs in cleaning release subregion；
C, release：Trap aedes albopictus, statistics BG-Trap adult mosquito trapping in point of release with BG-Trap adult mosquito trap
The male mosquito that device is traped, then enters performing PCR detection, judges whether it carries culex Wolbachia, unite respectively to male mosquito
Meter carries the positive hero mosquito number of culex Wolbachia and does not carry the negative hero mosquito number of culex Wolbachia, is released
Put ratio, the moon of the positive hero mosquito number of releasing ratio=carry culex Wolbachia/do not carry culex Wolbachia
Property male mosquito number, then according to releasing ratio is more than or equal to 5 positives carrying culex Wolbachia in point of release fixed point release
Male mosquito.
Trap aedes albopictus, statistics BG-Trap adult mosquito trap trapping in point of release with BG-Trap adult mosquito trap
The male mosquito arriving, and carry out fluorescence quantitative PCR detection, the Aedes albopictus in field do not carry the Wolbachia of culex, release
Aedes albopictus carry culex Wolbachia, according to this feature, design the specific primer of culex Wolbachia,
wPipF:5’-GTTTGTGCAGCTAATAG-3’；wPipR:5’-GTCTGCAAGGCCTATTTCTACTG-3’；Fixed by fluorescence
Amount PCR can distinguish the Aedes albopictus in the field that BG-Trap traps and the Aedes albopictus quantity of release, calculates release ratio
Example.
Preferably, it is to be equal to 5 according to releasing ratio to carry the positive male of culex Wolbachia in point of release fixed point release
Mosquito.
The aedes albopictus fixed point subregion method for releasing carrying Wolbachia of the present invention, it is mainly according to landform ground
Looks and the environment of surrounding, near breeding place and possible habitat (such as vegetation is luxuriant, and flow of the people is big, the water source of abundance)
Discharge, so give priority in the case that each area distribution that ensures to try one's best is average, and can be according to field again
Mosquito quantity and place adjust in time, and sensitivity is higher and reduces mosquito loss to greatest extent, and is distributed more in mosquito
Place also can reach good pressing result.
Specific embodiment：
Following examples are that the present invention is further illustrated, rather than limitation of the present invention.
Embodiment 1：
The egg apparatus that lures in the present embodiment is purchased from East South Industry Co., Ltd. ,Guangdong Province, and its name of product is Mosq-ovitrap.
BG-Trap adult mosquito trap in the present embodiment is purchased from Shanghai Qi Dong Industrial Co., Ltd., and its name of product is
QD- II type adult mosquito trapper.
A, we by have between each for Sha Zai island landform natural obstruct region division be 22 release subregions, basic guarantee
Each discharges the relatively independent of subregion.In each release subregion, according to the ambient conditions of different terrain and surrounding, select mosquito
, nearby as suitable point of release, described habitat is humid environment for the breeding place of worm and habitat, and shading and vegetation are luxuriant
The place contained.
It is filled with water and container that mosquito may lay eggs in b, cleaning release subregion, that is, need to dispose permissible in all regions
Hydrops in water container, some cannot be removed or the breeding place of stubbornness needs medication to be processed.
Each zone leveling of c, Sha Zai island has the point of release of 8 about, in point of release BG-Trap adult mosquito trap
Trapping aedes albopictus, the male mosquito that statistics BG-Trap adult mosquito trap is traped, then enters performing PCR detection, judges it to male mosquito
Whether carry culex Wolbachia, statistics carries the positive hero mosquito number of culex Wolbachia and do not carry culex respectively
The negative hero mosquito number of Wolbachia, is released ratio, releasing ratio=carry the positive male of culex Wolbachia
The negative hero mosquito number of mosquito number/do not carry culex Wolbachia, then according to releasing ratio is equal to 5 pinpoints release in point of release
Carry the positive hero mosquito of culex Wolbachia.
Described enters performing PCR detection to male mosquito, and the fluorescent detector primers of its culex Wolbachia are：
wPipF:5’-GTTTGTGCAGCTAATAG-3’；
wPipR:5’-GTCTGCAAGGCCTATTTCTACTG-3’.
With luring egg apparatus and BG-Trap adult mosquito trap that Aedes albopictus are carried out with monitor in real time after release, concrete outcome is joined
It is shown in Table 1：
Comparison little Hu island is by the way of discharge the aedes albopictus hero mosquito carrying culex Wolbachia when walking.
Table in June, 1 2016 Sha Zai Dao He little Hu island pressing result
As it can be seen from table 1 the pressing result of fixed point release is better than the pattern put when walking.With check plot little Hu island phase
Substantially all reach 85% about than, the ovum pressing result of Sha Zaidao and adult mosquito pressing result.And the current positive in Sha Zai island is multiplied
The Radix Rehmanniae has greatly reduced.
Claims (2)
1. a kind of aedes albopictus fixed point subregion method for releasing carrying Wolbachia is it is characterised in that comprise the following steps：
A, prospecting landform：In release subregion, according to the ambient conditions of different terrain and surrounding, select mosquito breeding place and
Nearby as point of release, described habitat is humid environment to habitat, shading and the luxuriant place of vegetation；
B, cleaning breeding place：It is filled with water and container that mosquito may lay eggs in cleaning release subregion；
C, release：Trap aedes albopictus in point of release with BG-Trap adult mosquito trap, count BG-Trap adult mosquito trap
The male mosquito traped, then enters performing PCR detection, judges whether it carries culex Wolbachia, statistics is taken respectively to male mosquito
Positive hero mosquito number with culex Wolbachia and the negative hero mosquito number not carrying culex Wolbachia, are released comparing
Example, the positive hero mosquito number of releasing ratio=carry culex Wolbachia/do not carry the negative male of culex Wolbachia
Mosquito number, then according to releasing ratio is more than or equal to the 5 positive hero mosquitos carrying culex Wolbachia in point of release fixed point release.
2. the aedes albopictus fixed point subregion method for releasing carrying Wolbachia according to claim 1, its feature exists
In,
Described enters performing PCR detection to male mosquito, and the fluorescent detector primers of its culex Wolbachia are：
wPipF:5’-GTTTGTGCAGCTAATAG-3’；
wPipR:5’-GTCTGCAAGGCCTATTTCTACTG-3’.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610768629.XA CN106386779A (en) | 2016-08-30 | 2016-08-30 | Oriental mosquito fixed-point partition release method carrying Wolbachia |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610768629.XA CN106386779A (en) | 2016-08-30 | 2016-08-30 | Oriental mosquito fixed-point partition release method carrying Wolbachia |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106386779A true CN106386779A (en) | 2017-02-15 |
Family
ID=58003120
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610768629.XA Withdrawn CN106386779A (en) | 2016-08-30 | 2016-08-30 | Oriental mosquito fixed-point partition release method carrying Wolbachia |
Country Status (1)
| Country | Link |
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| CN (1) | CN106386779A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019028584A1 (en) * | 2017-08-07 | 2019-02-14 | 广州威佰昆生物科技有限公司 | Aedes albopictus and production method therefor |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006008652A1 (en) * | 2004-07-15 | 2006-01-26 | Institute Of Molecular Biology And Biotechnology -Forth | Insect population control |
| US7868222B1 (en) * | 2006-03-10 | 2011-01-11 | University Of Kentucky Research Foundation | Transfected mosquito vectors |
| CN104878112A (en) * | 2015-06-11 | 2015-09-02 | 奚志勇 | Fluorescence detection primer of culex wolbachia, detection method thereof and detection kit |
-
2016
- 2016-08-30 CN CN201610768629.XA patent/CN106386779A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006008652A1 (en) * | 2004-07-15 | 2006-01-26 | Institute Of Molecular Biology And Biotechnology -Forth | Insect population control |
| US7868222B1 (en) * | 2006-03-10 | 2011-01-11 | University Of Kentucky Research Foundation | Transfected mosquito vectors |
| CN104878112A (en) * | 2015-06-11 | 2015-09-02 | 奚志勇 | Fluorescence detection primer of culex wolbachia, detection method thereof and detection kit |
Non-Patent Citations (4)
| Title |
|---|
| A.A.HOFFMANN，ETC.: "Successful establishment of Wolbachia in Aedes populations to suppress dengue transmission", 《NATURE》 * |
| ERIC P.CARAGATA，ETC.: "Exploiting Intimate Relationships:Controlling Mosquito-Transmitted Disease with Wolbachia", 《CELL PRESS》 * |
| JASON L.RASGON,ETC.: "Impact of population age structure on Wolbachia transgene driver efficacy ecologically complex factors and release of genetically modified mosquitos", 《INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY》 * |
| 苏善强等: "海南岛大劣按蚊扩散的实验观察", 《寄生虫学与寄生虫病杂志》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019028584A1 (en) * | 2017-08-07 | 2019-02-14 | 广州威佰昆生物科技有限公司 | Aedes albopictus and production method therefor |
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| PB01 | Publication | ||
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Application publication date: 20170215 |