CN106386501A - Chirita pinnatifida tissue culture method - Google Patents
Chirita pinnatifida tissue culture method Download PDFInfo
- Publication number
- CN106386501A CN106386501A CN201610975345.8A CN201610975345A CN106386501A CN 106386501 A CN106386501 A CN 106386501A CN 201610975345 A CN201610975345 A CN 201610975345A CN 106386501 A CN106386501 A CN 106386501A
- Authority
- CN
- China
- Prior art keywords
- culture
- blade
- tissue culture
- root
- seedling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to the technical field of bioengineering and particularly relates to a Chirita pinnatifida tissue culture method. The Chirita pinnatifida tissue culture method includes the steps of explant sterilization, adventitious bud induction, subculture, rooting culture and acclimatization and transplant. The Chirita pinnatifida tissue culture method has the advantages of good offspring growth vigor, a large number of adventitious buds, high reproduction speed, large reproduction coefficient, uniformity in propagated offspring, high rooting rate and high transplanting survival rate.
Description
Technical field
The invention belongs to technical field of bioengineering is and in particular to a kind of method for tissue culture of plumage split lip post lettuce tongue.
Background technology
Plumage split lip post lettuce tongue (Chirita pinnatifida (Hand.-Mazz.) B.L.Burtt) is Gesneriaceae lip post
The herbaceos perennial that lettuce tongue belongs to.Be distributed in Guangxi, North Guangdong, Dong people, Southern Hunan, Jiangxi, In Western Fujian Province,
Southern Zhejiang and western part.It is born on stone in the paddy woods of height above sea level 600-1500 rice or small stream side.Herb can hyoscine, for traumatic injury
Deng disease.Additionally, plumage split lip post lettuce tongue also has high ornamental value, have a extensive future.
Plumage split lip post lettuce tongue cultivation difficulty is larger, harsh to growth environmental requirement.Need high air humidity, high soil wet
Degree, but same time root system gasifies to the soil water, oxygen content has strict demand；Avoid direct sunlight, happiness weak acid is to neutral cultivation base
Matter；Fear heat；The happiness summer higher day and night temperature difference, summer day temperature maximum temperature is preferably no more than 30 DEG C.
At present, the research about plumage split lip post lettuce tongue is few, only has in resource investigation and is related on a small quantity.Plumage split lip post lettuce tongue
Seminal propagation or blade cuttage can be adopted, and tissue culture technique has, and reproduction speed is fast, breeding coefficient big, raises up seed whole
Homogeneous causes, and can keep the merit of original kind, the advantages of restriction by season, have been widely used various ornamental plants
In, have not yet to see and be applied on plumage split lip post lettuce tongue.
Content of the invention
For the weak point of the existing raising technology of plumage split lip post lettuce tongue, the present invention provides a kind of group of plumage split lip post lettuce tongue
Knit cultural method, such that it is able to reach the quick purpose obtaining plumage split lip post lettuce tongue high quality seedling.
In order to realize foregoing invention purpose, the present invention employs the following technical solutions：
A kind of method for tissue culture of plumage split lip post lettuce tongue, comprises the steps：
(1) sterilization of explant：Spring takes the young leaflet tablet of plumage split lip post lettuce tongue, first uses 0.1-0.5% detergent
Rinse 30min under flowing water after solution soaking 25-30min, and gently scrub blade with banister brush, then proceed to superclean bench
Carry out disinfecting action, first dip 75% ethanol with cotton balls and wipe blade surface, aseptic water washing 2 times, by blade in 75% ethanol
Middle immersion 10s, aseptic water washing 3 times, then process 6min, aseptic water washing 3 times with 0.1% mercuric chloride solution, finally use again
0.1% mercuric chloride solution processes 6min, aseptic water washing 3 times, and in mercuric chloride sterilization process, gently concussion makes mercuric chloride fully connect
Tactile blade；Blade is cut into 2cm × 2cm size, is seeded in MS culture medium, obtains aseptic blade；
(2) induction of adventitious bud：The aseptic blade of acquisition is cut into 1cm × 1cm size, and leaves in blade surrounding and cut
Mouthful, it is seeded in adventitious bud induction culture base, through the culture of 20-25d, the wound of blade surrounding induces adventitious bud；
(3) successive transfer culture：The adventitious bud of acquisition is cut, is seeded in culture 25-30d in subculture medium, obtains
Culturing young plants；
(4) root culture：Choose the culturing young plants that height is 1.5-3.0cm, be seeded in culture 18- in root media
20d, obtains tissue culture and takes root Seedling；
(5) acclimatization and transplantses：Bottle cap is thrown off in natural bar after the Seedling removal culturing room of taking root height being 2.5-4.0cm
Part lower refining seedling 4 is cleaned culture medium and is transplanted to equipped with 50 hole disks of substrate；After irrigating root water, it has been placed in screening by transplanting seedling
Under the cool canopy of screened postive, light transmittance is 40-50%, and controls air humidity using intermittent spraying system, and every 2h sprays 1 time, spray
Mist time each 40s, it is ensured that air humidity more than 85%, pours permeable each 1 time, 7-10d after transplanting, you can survive sooner or later.
Preferably, the adventitious bud induction culture base in step (2) is MS+6-BA1.0-2.0mg/L+NAA0.01-
0.1mg/L.
Preferably, the subculture medium in step (3) is MS+6-BA1.0-2.0mg/L+NAA0.05mg/L+ activated carbon
0.1g/L.
Preferably, the root media in step (4) is MS+IBA0.1-0.5mg/L+ activated carbon 0.1g/L.
Preferably, the substrate in step (5) is to be 2 by peat and perlite according to volume ratio:1 composition.
Compared with prior art, the present invention has the advantages that：
The method for tissue culture of the plumage split lip post lettuce tongue of the present invention has offspring and grows fine, and adventitious bud is many, reproduction speed
Hurry up, breeding coefficient big, raise up seed neat and consistent, and rooting rate is high, the high advantage of transplanting survival rate.
Specific embodiment
Following examples are used for the present invention is described, but are not limited to the scope of the present invention.Without departing substantially from present invention spirit
In the case of essence, the modification that the inventive method, step or condition are made or replacement, belong to the scope of the present invention.
Embodiment 1：
A kind of method for tissue culture of plumage split lip post lettuce tongue, comprises the steps：
(1) sterilization of explant：Spring takes the young leaflet tablet of plumage split lip post lettuce tongue, first uses 0.1% washing powder solution
Rinse 30min well under flowing water after soaking 25min, and gently scrub blade with banister brush, then proceed to superclean bench and enter
Row disinfecting action, first dips 75% ethanol with cotton balls and wipes blade surface, aseptic water washing 2 times, by blade in 75% ethanol
Soak 10s, aseptic water washing 3 times, then process 6min with 0.1% mercuric chloride solution, aseptic water washing 3 times, finally again with 0.1%
Mercuric chloride solution processes 6min, aseptic water washing 3 times, and in mercuric chloride sterilization process, gently concussion makes mercuric chloride be fully contacted leaf
Piece；Blade is cut into 2cm × 2cm size, is seeded in MS culture medium, obtains aseptic blade；
(2) induction of adventitious bud：The aseptic blade of acquisition is cut into 1cm × 1cm size, and leaves in blade surrounding and cut
Mouthful, it is seeded in adventitious bud induction culture base, through the culture of 21d, the wound of blade surrounding induces adventitious bud；Described not
Normal bud inducing culture is MS+6-BA 1.0mg/L+NAA 0.01mg/L；
(3) successive transfer culture：The adventitious bud of acquisition is cut, is seeded in culture 25d in subculture medium, obtains tissue culture
Seedling；Described subculture medium is MS+6-BA 1.0mg/L+NAA 0.05mg/L+ activated carbon 0.1g/L；
(4) root culture：Choose the culturing young plants that height is 1.5cm, be seeded in culture 20d in root media；Described
Root media be MS+IBA 0.1mg/L+ activated carbon 0.1g/L；
(5) acclimatization and transplantses：Take off lid seedling exercising 4d under field conditions (factors) by after the Seedling removal culturing room of taking root of a height of 2.5cm；Wash
Net culture medium is transplanted to equipped with 50 hole disks of substrate；After irrigating root water, transplanting seedling is placed in the cool canopy of sunshade net
Under, light transmittance is 40%, and controls air humidity using intermittent spraying system, and every 2h sprays 1 time, each 40s of spray time,
Guarantee air humidity more than 85%, sooner or later pour permeable each 1 time, 7d after transplanting, you can survive；Described substrate is by peat and treasure
Zhu Yan is 2 according to volume ratio:1 composition.
Embodiment 2：
A kind of method for tissue culture of plumage split lip post lettuce tongue, comprises the steps：
(1) sterilization of explant：Spring takes the young leaflet tablet of plumage split lip post lettuce tongue, first uses 0.5% washing powder solution
Rinse 30min under flowing water after soaking 30min, and gently scrub blade with banister brush, then proceed to superclean bench and gone out
Bacterium operates, and first dips 75% ethanol with cotton balls and wipes blade surface, aseptic water washing 2 times, blade is soaked in 75% ethanol
10s, aseptic water washing 3 times, then process 6min, aseptic water washing 3 times with 0.1% mercuric chloride solution, finally use 0.1% chlorination again
Mercury solution processes 6min, aseptic water washing 3 times, and in mercuric chloride sterilization process, gently concussion makes mercuric chloride be fully contacted blade；Leaf
Piece is cut into 2cm × 2cm size, accesses MS culture medium, obtains aseptic blade；
(2) induction of adventitious bud：The aseptic blade of acquisition is cut into 1cm × 1cm size, and leaves in blade surrounding and cut
Mouthful, it is seeded in adventitious bud induction culture base, through the culture of 20d, the wound of blade surrounding induces adventitious bud；Described not
Normal bud inducing culture is MS+6-BA 2.0mg/L+NAA 0.01mg/L；
(3) successive transfer culture：The adventitious bud of acquisition is cut, is seeded in culture 30d in subculture medium, obtains tissue culture
Seedling；Described subculture medium is MS+6-BA2.0mg/L+NAA 0.05mg/L+ activated carbon 0.1g/L；
(4) root culture：Choose the culturing young plants that height embarrasses 3.0cm, be seeded in culture 18d in root media；Institute
The root media stated is MS+IBA 0.5mg/L+ activated carbon 0.1g/L；
(5) acclimatization and transplantses：Take off lid seedling exercising 5d under field conditions (factors) by after the Seedling removal culturing room of taking root of a height of 4.0cm；Wash
Net culture medium is transplanted to equipped with 50 hole disks of substrate；After irrigating root water, transplanting seedling is placed in the cool canopy of sunshade net
Under, light transmittance is 50%, and controls air humidity using intermittent spraying system, and every 2h sprays 1 time, each 40s of spray time,
Guarantee air humidity more than 85%, sooner or later pour permeable each 1 time, 10d after transplanting, you can survive；Described substrate be by peat and
Perlite is 2 according to volume ratio:1 composition.
Embodiment 3：
A kind of method for tissue culture of plumage split lip post lettuce tongue, comprises the steps：
(1) sterilization of explant：Spring takes the young leaflet tablet of plumage split lip post lettuce tongue, first uses 0.2% washing powder solution
Rinse 30min well under flowing water after soaking 28min, and gently scrub blade with banister brush, then proceed to superclean bench and enter
Row disinfecting action, first dips 75% ethanol with cotton balls and wipes blade surface, aseptic water washing 2 times, by blade in 75% ethanol
Soak 10s, aseptic water washing 3 times, then process 6min with 0.1% mercuric chloride solution, aseptic water washing 3 times, finally again with 0.1%
Mercuric chloride solution processes 6min, aseptic water washing 3 times, and in mercuric chloride sterilization process, gently concussion makes mercuric chloride be fully contacted leaf
Piece；Blade is cut into 2cm × 2cm size, is seeded in MS culture medium, obtains aseptic blade；
(2) induction of adventitious bud：The aseptic blade of acquisition is cut into 1cm × 1cm size, and leaves in blade surrounding and cut
Mouthful, it is seeded in adventitious bud induction culture base, through the culture of 25d, the wound of blade surrounding induces adventitious bud；Described not
Normal bud inducing culture is MS+6-BA 1.0mg/L+NAA 0.1mg/L；
(3) successive transfer culture：The adventitious bud of acquisition is cut, is seeded in culture 26d in subculture medium, obtains tissue culture
Seedling；Described subculture medium is MS+6-BA 1.5mg/L+NAA 0.05mg/L+ activated carbon 0.1g/L；
(4) root culture：Choose the culturing young plants that height is 2.0cm, be seeded in culture 19d in root media；Described
Root media be MS+IBA 0.3mg/L+ activated carbon 0.1g/L；
(5) acclimatization and transplantses：Take off lid seedling exercising 4d under field conditions (factors) by after the Seedling removal culturing room of taking root of a height of 3.0cm；Wash
Net culture medium is transplanted to equipped with 50 hole disks of substrate；After irrigating root water, transplanting seedling is placed in the cool canopy of sunshade net
Under, light transmittance is 45%, and controls air humidity using intermittent spraying system, and every 2h sprays 1 time, each 40s of spray time,
Guarantee air humidity more than 85%, sooner or later pour permeable each 1 time, 8d after transplanting, you can survive；Described substrate is by peat and treasure
Zhu Yan is 2 according to volume ratio:1 composition.
Induce aerosor in the method for tissue culture of the plumage split lip post lettuce tongue that above-described embodiment 1-3 is obtained, adventitious bud
The transplanting survival rate of subculture increment, adventitious bud rooting situation and tissue cultured seedling carries out investigation statisticses, the results are shown in Table 1.
The method for tissue culture of the plumage split lip post lettuce tongue of table 1 present invention rises in value to the subculture of Induce aerosor, adventitious bud, no
Normal bud takes root the mensure of situation
As shown in Table 1, the adventitious bud that the method for tissue culture of the plumage split lip post lettuce tongue of the present invention obtains grows fine, indefinite
Bud is many；Its inductivity and rooting rate are 100%, and the transplanting survival rate of tissue cultured seedling is 100%.It can be seen that, the method for the present invention has
There are obvious technical advantage, worth further genralrlization and application.
The description of the aforementioned specific illustrative embodiment to the present invention illustrate that and illustration purpose.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to above-mentioned teaching, can much be changed
And change.The purpose of selecting and describing the exemplary embodiment is that explaining that the certain principles of the present invention and its reality should
With so that those skilled in the art be capable of and utilize the present invention various different exemplary and
Various different selections and change.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (5)
1. a kind of method for tissue culture of plumage split lip post lettuce tongue is it is characterised in that comprise the steps：
(1) sterilization of explant：Spring takes the young leaflet tablet of plumage split lip post lettuce tongue, first uses 0.1-0.5% washing powder solution
Rinse 30min under flowing water after soaking 25-30min, and gently scrub blade with banister brush, then proceed to superclean bench and carry out
Disinfecting action, first dips 75% ethanol with cotton balls and wipes blade surface, aseptic water washing 2 times, then by blade in 75% ethanol
Middle immersion 10s, aseptic water washing 3 times, then process 6min, aseptic water washing 3 times with 0.1% mercuric chloride solution, finally use again
0.1% mercuric chloride solution processes 6min, aseptic water washing 3 times, and in mercuric chloride sterilization process, gently concussion makes mercuric chloride fully connect
Tactile blade；Blade is cut into 2cm × 2cm size, is seeded in MS culture medium, obtains aseptic blade；
(2) induction of adventitious bud：The aseptic blade of acquisition is cut into 1cm × 1cm size, and leaves otch in blade surrounding, connect
Plant in adventitious bud induction culture base, through the culture of 20-25d, induce adventitious bud from the wound of blade surrounding；
(3) successive transfer culture：The adventitious bud of acquisition is cut, is seeded in culture 25-30d in subculture medium, obtains tissue culture
Seedling；
(4) root culture：Choose the culturing young plants that height is 1.5-3.0cm, be seeded in culture 18-20d in root media, obtain
Take root Seedling to tissue culture；
(5) acclimatization and transplantses：Bottle cap is thrown off under field conditions (factors) after the Seedling removal culturing room of taking root height being 2.5-4.0cm
Seedling exercising 4-5d；Clean culture medium is transplanted to equipped with 50 hole disks of substrate；After irrigating root water, it has been placed in screening by transplanting seedling
Under the cool canopy of screened postive, light transmittance is 40-50%, and controls air humidity using intermittent spraying system, and every 2h sprays 1 time, spray
Mist time each 40s, it is ensured that air humidity more than 85%, pours permeable each 1 time, 7-10d after transplanting, you can survive sooner or later.
2. the method for tissue culture of plumage split lip post lettuce tongue according to claim 1 is it is characterised in that in step (2) not
Normal bud inducing culture is MS+6-BA1.0-2.0mg/L+NAA0.01-0.1mg/L.
3. the method for tissue culture of plumage split lip post lettuce tongue according to claim 1 is it is characterised in that continuing in step (3)
Culture base is MS+6-BA1.0-2.0mg/L+NAA0.05mg/L+ activated carbon 0.1g/L.
4. the method for tissue culture of plumage split lip post lettuce tongue according to claim 1 is it is characterised in that life in step (4)
Root culture medium is MS+IBA0.1-0.5mg/L+ activated carbon 0.1g/L.
5. the method for tissue culture of plumage split lip post lettuce tongue according to claim 1 is it is characterised in that base in step (5)
Matter is to be 2 by peat and perlite according to volume ratio:1 composition.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610975345.8A CN106386501A (en) | 2016-11-07 | 2016-11-07 | Chirita pinnatifida tissue culture method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610975345.8A CN106386501A (en) | 2016-11-07 | 2016-11-07 | Chirita pinnatifida tissue culture method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106386501A true CN106386501A (en) | 2017-02-15 |
Family
ID=58015558
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610975345.8A Pending CN106386501A (en) | 2016-11-07 | 2016-11-07 | Chirita pinnatifida tissue culture method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106386501A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107047302A (en) * | 2017-03-24 | 2017-08-18 | 广西壮族自治区农业科学院花卉研究所 | A kind of method for tissue culture of Guigang primulina tabacum |
| CN112106657A (en) * | 2020-09-25 | 2020-12-22 | 西南林业大学 | Tissue culture method of chirita mossambica |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103461129A (en) * | 2013-09-17 | 2013-12-25 | 中国科学院华南植物园 | Tissue culture and intermediate propagation method of chirita anachoreta |
| CN103651134A (en) * | 2013-12-05 | 2014-03-26 | 天津滨海国际花卉科技园区股份有限公司 | Tissue culture method for chirita wentsaii |
| CN104823855A (en) * | 2015-05-18 | 2015-08-12 | 贵州省林业科学研究院 | Chirita brachytricha tissue culture and rapid propagation method |
-
2016
- 2016-11-07 CN CN201610975345.8A patent/CN106386501A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103461129A (en) * | 2013-09-17 | 2013-12-25 | 中国科学院华南植物园 | Tissue culture and intermediate propagation method of chirita anachoreta |
| CN103651134A (en) * | 2013-12-05 | 2014-03-26 | 天津滨海国际花卉科技园区股份有限公司 | Tissue culture method for chirita wentsaii |
| CN104823855A (en) * | 2015-05-18 | 2015-08-12 | 贵州省林业科学研究院 | Chirita brachytricha tissue culture and rapid propagation method |
Non-Patent Citations (5)
| Title |
|---|
| 侯娜等: "荔波唇柱苣苔离体叶片不定芽的诱导及植株再生", 《林业科技开发》 * |
| 刘弘: "《植物组织培养技术》", 29 February 2012, 机械工业出版社 * |
| 姚绍嫦等: "药用唇柱苣苔叶片分化及植株再生研究", 《种子》 * |
| 潘梅等: "烟叶唇柱苣苔叶片分化与植株再生研究", 《北方园艺》 * |
| 石晓东等: "《植物组织培养》", 31 August 2013, 中国农业科学技术出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107047302A (en) * | 2017-03-24 | 2017-08-18 | 广西壮族自治区农业科学院花卉研究所 | A kind of method for tissue culture of Guigang primulina tabacum |
| CN107047302B (en) * | 2017-03-24 | 2019-04-05 | 广西壮族自治区农业科学院花卉研究所 | A kind of method for tissue culture of Guigang primulina tabacum |
| CN112106657A (en) * | 2020-09-25 | 2020-12-22 | 西南林业大学 | Tissue culture method of chirita mossambica |
| CN112106657B (en) * | 2020-09-25 | 2021-04-20 | 西南林业大学 | Tissue culture method of chirita mossambica |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101647393A (en) | Fast tissue culture reproducing method of actinidia eriantha | |
| CN104920228B (en) | A kind of Baoxing Bulbus Lilii tissue-culturing rapid propagation and in-vitro conservation method | |
| CN101366357A (en) | Method for tissue culture and quick propagate technique of reddish blue spider lily | |
| CN104012417B (en) | High-efficiency and rapid micropropagation method for toxicodendron vernicifluum | |
| CN112690213A (en) | Tissue culture and rapid propagation method for high-quality seedlings of hippeastrum rutilum | |
| CN106106163A (en) | A kind of iris cultivates propagation method | |
| CN103688855B (en) | A kind of leaflet red bean isolated seed embryo and plant regeneration method | |
| CN102217540A (en) | Quick propagation method for lycoris chinensis | |
| CN103430842B (en) | A kind of Quick propagation method of hybrid orchid tissue culture | |
| CN104604677B (en) | A kind of tissue culture propagation method reducing russian dandelion tissue culture of Russia melting brown rate | |
| CN102217551B (en) | Tissue culture quick reproduction method for dendrobium chrysotoxum lindl bud tips | |
| CN102657094A (en) | Ex-vitro soilless cutting rooting method for tissue-cultured and proliferated seedlings of gerbera jamesonii bolus | |
| CN103141381A (en) | Tissue culture method for oriental lily | |
| CN106538392A (en) | A kind of white oriental cherry tissue culture and rapid proliferation method | |
| CN106386501A (en) | Chirita pinnatifida tissue culture method | |
| CN106718888B (en) | A kind of in-vitro culture method of brown line primulina tabacum | |
| CN113115708A (en) | Method for regenerating peony in-vitro plant and application thereof | |
| CN109496858B (en) | Tissue culture and rapid propagation method of amomum tsao-ko | |
| CN108077066A (en) | A kind of caladium bicolor somatic embryogenesis pathway tissue culture and rapid propagation method | |
| CN108142281A (en) | A kind of Cortex Eucommiae method for tissue culture | |
| CN107006367B (en) | 'sunshine' cherry tissue culture rapid propagation method | |
| Sherif et al. | Regeneration of plantlets from nodal and shoot tip explants of Anoectochilus elatus Lindley, an endangered terrestrial orchid | |
| CN104206071A (en) | Rapid propagation method of alder | |
| CN115250915A (en) | Efficient propagation method for stem section induced protocorm and plant regeneration of dendrobium tibetanum | |
| CN108094200A (en) | A kind of be heat-treated combines the breeding method that stem apex stripping acquisition peace ancestral spends detoxic seedling |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170215 |