CN106376606A - Sunflower floral disc bacterial depressant, preparation method and application of sunflower floral disc bacterial depressant for preventing and treating potato early blight - Google Patents
Sunflower floral disc bacterial depressant, preparation method and application of sunflower floral disc bacterial depressant for preventing and treating potato early blight Download PDFInfo
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- CN106376606A CN106376606A CN201610779488.1A CN201610779488A CN106376606A CN 106376606 A CN106376606 A CN 106376606A CN 201610779488 A CN201610779488 A CN 201610779488A CN 106376606 A CN106376606 A CN 106376606A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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Abstract
The invention provides a sunflower floral disc bacterial depressant. According to the invention, the Sunflower floral disc is taken as a raw material, is extracted by a solvent to obtain a sunflower floral disc extract product, and the solvent is water, ethyl acetate or petroleum ether. A preparation method comprises the following steps: 1) crushing sunflower floral disc to obtain the sunflower floral disc powder; 2) mixing the sunflower floral disc powder and the solvent, extracting a mixture to obtain leaching liquor, wherein the solvent is water, ethyl acetate or petroleum ether; 3) centrifuging the leaching liquor obtained in the step 2) to obtain a supernatant as the sunflower floral disc extract product; and 4) mixing the sunflower floral disc extract in the step 3) and an accessory accepted in field of pesticides to obtain the sunflower floral disc bacterial depressant. According to an application of the sunflower floral disc bacterial depressant for preventing and treating potato early blight, the bacteriostasis rate of the sunflower floral disc bacterial depressant is 24.16%-45.90%.
Description
Technical field
The present invention relates to technical field of pesticide and in particular to a kind of floral disc of sunflower extract and preparation method thereof and preventing and treating
The application of target.
Background technology
Target (Alternaria solani Sorauer) is mutually every interlinkage spore (Alternaria
Alternata a kind of worldwide disease) causing, is distributed more universal in China Rhizoma Solani tuber osi producing region.Early blight mostly occurs in horse
Bell potato Later growth, causes blade to dry up in advance, yield reduction, individually the serious full Tian Wushou in plot.In recent years, early blight is being permitted
Many Rhizoma Solani tuber osis producing region is frequently anti-raw and on the rise, and the general time can cause the Rhizoma Solani tuber osi underproduction 5%～10%, serious time
Output reduction is up to more than 50%.Also become the one of Potato Industry by the storage period horse good several piece stem rot mutually causing every interlinkage spore is rotten
Individual serious problems, in some areas, storage tuber loss reaches 30%.Target is in a bad way, and production loss is bigger,
When disease is seriously 50%, production loss 20%, when disease severity is 100%, production loss is 40%.
In actual production, the Potato Cultivars of anti-early blight are less, rely primarily on Agro-chemicals control, conventional sterilization
Agent mainly has Mancozeb, Bravo, Fluoxastrobin etc., but these Agro-chemicals control effects are undesirable, and use cost is higher,
And abroad report mutually develops immunity to drugs every interlinkage spore Fluoxastrobin.In order to improve prevention effect and reduces cost, prior art
In often carry out mixture using the antibacterial in producing, to delay the drug-fast generation of pathogenic bacteria, such as Tai Lianmei et al. studies mixture
(mixture antibacterial is to the preventive effect of target and to product for the preventive effect to target for the antibacterial and the impact to yield
The impact of amount, Tai Lianmei, Jiangsu's agriculture science, the 9th phase of volume 42,2014：110-112), mixed using Fluoxastrobin and Mancozeb
With reaching mutual supplement with each other's advantages, but mixture antibacterial still can promote pathogenic bacteria to develop immunity to drugs.
Content of the invention
In view of this, it is an object of the invention to provide a kind of plant-based bacteriostat-floral disc of sunflower extract, make described
Floral disc of sunflower extract has inhibitory action to the mutual of the target causing every interlinkage spore, thus reaching early to Rhizoma Solani tuber osi
The purpose of the preventive effect of epidemic disease.
In order to realize foregoing invention purpose, the present invention provides technical scheme below：
The invention provides floral disc of sunflower antibacterial, solvent extraction is adopted to obtain including with floral disc of sunflower for raw material
Floral disc of sunflower extract, described solvent is water, ethyl acetate or petroleum ether.
Preferably, the quality of described floral disc of sunflower and the volume ratio of solvent are 1kg：(5～15) L.
Preferably, the method for described extraction comprises the following steps：
1) floral disc of sunflower is pulverized, the floral disc of sunflower powder obtaining；
2) described floral disc of sunflower powder is mixed with solvent, extraction, obtain lixiviating solution；
3) by described step 2) centrifugation of the lixiviating solution that obtains, the supernatant obtaining is floral disc of sunflower antibacterial.
Preferably, the time of described extraction is 3～5d.
Preferably, described step 3) in centrifugation after also include：The supernatant obtaining is concentrated and is dried.
The present invention also provides a kind of preparation method of described floral disc of sunflower antibacterial, comprises the following steps：
1) floral disc of sunflower is pulverized, the floral disc of sunflower powder obtaining；
2) described floral disc of sunflower powder is mixed with solvent, extraction, obtain lixiviating solution, described solvent is water, acetic acid second
Ester or petroleum ether；
3) by described step 2) centrifugation of the lixiviating solution that obtains, the supernatant obtaining is floral disc of sunflower extract；
4) by described step 3) adjuvant that received with pesticide field of the floral disc of sunflower extracting solution that obtains mixed, obtains to day
Receptaculum Helianthi antibacterial.
Preferably, described step 2) in floral disc of sunflower powder before mixing with solvent cross 60～100 mesh sieves.
Preferably, described step 3) in centrifugation rotating speed be 2000～4000r/min rotating speed；Described step 3) in centrifugation
Time be 10～20min.
Present invention also offers application in inhibition of potato early epidemic pathogenic bacteria for the described floral disc of sunflower antibacterial.
Preferably, in described floral disc of sunflower antibacterial, the concentration of floral disc of sunflower extract is 40～640mg/ml.
The invention provides floral disc of sunflower antibacterial, solvent extraction is adopted to obtain including with floral disc of sunflower for raw material
Floral disc of sunflower extract, described solvent is water, ethyl acetate or petroleum ether.Solvent extraction Flos Helianthi using opposed polarity
Different types of active substance in disk, the active matter of the floral disc of sunflower that gained water, ethyl acetate or Petroleum ether extraction obtain
Matter all has bacteria resistance function.
Meanwhile, the invention provides floral disc of sunflower antibacterial, the raw material sources of extraction extensively, are readily available, by inciting somebody to action
Floral disc of sunflower extracts antibacterial substance, and floral disc of sunflower is turned waste into wealth, and decreases floral disc of sunflower because burning to environment simultaneously
The pollution causing, also reduces the processing cost of floral disc of sunflower.
The present invention also provides a kind of preparation method of described floral disc of sunflower antibacterial, after floral disc of sunflower is pulverized
The powder arriving is mixed with solvent, extraction, centrifugation, and the supernatant obtaining is floral disc of sunflower antibacterial.Described preparation method has
Method is simple, easy and simple to handle, not high to the technical requirements of operator, the reproducible feature of preparation method.
The present invention also provides application in inhibition of potato early epidemic pathogenic bacteria for the described floral disc of sunflower antibacterial.Described to
Day Receptaculum Helianthi antibacterial can effectively suppress to cause the mutual growth every interlinkage spore of target, thus reaching to Rhizoma Solani tuber osi
The inhibitory action of early blight, the bacteriostasis rate of described floral disc of sunflower antibacterial is 24.16%～45.90%.
Further, floral disc of sunflower antibacterial of the present invention when mass concentration is 160～640mg/mL to drawing
The mutual antibacterial circle diameter every interlinkage spore playing target is 10.68～11.5mm, and floral disc of sunflower water extract is to Ma Ling
Potato early blight pathogen mutually every interlinkage spore highest suppression ratio be 40.23%；The highest suppression ratio of ethyl acetate extract is
38.98%；The highest suppression ratio of ligroin extraction is 45.90%.
Specific embodiment
The invention provides floral disc of sunflower antibacterial, with floral disc of sunflower for raw material using solvent extraction acquisition to day
Receptaculum Helianthi extract, described solvent is water, ethyl acetate or petroleum ether.
The invention provides floral disc of sunflower antibacterial, using the difference in the solvent extraction floral disc of sunflower of opposed polarity
The active substance of species, the active substance of the floral disc of sunflower that gained water, ethyl acetate or Petroleum ether extraction obtain all has antibacterial
Function.
In the present invention, the quality of described floral disc of sunflower and the volume ratio of solvent are preferably 1kg：(5～15) L, more excellent
Elect 1kg as：10L.
In the present invention, the method for described extraction preferably includes following steps：
1) floral disc of sunflower is pulverized, obtain floral disc of sunflower powder；
2) described floral disc of sunflower powder is mixed with solvent, extraction, obtain lixiviating solution；
3) by described step 2) centrifugation of the lixiviating solution that obtains, the supernatant obtaining is floral disc of sunflower extract.
In the present invention, described floral disc of sunflower selects no insect pest, the floral disc of sunflower going mouldy.
In the present invention, described floral disc of sunflower was preferably dried before pulverizing.The temperature of described drying is preferably
35～60 DEG C, more preferably 37～50 DEG C；The time dried is preferably 1～5h, more preferably 2～4h.
In the present invention, the method for described pulverizing is not particularly limited, using pulverizing well-known to those skilled in the art
Method.In the present invention, the described method realization pulverized by grinding.
In the present invention, described pulverize after floral disc of sunflower granularity be preferably 60～100 mesh, more preferably 65～80
Mesh.
Obtain sunflower disk meal last, described floral disc of sunflower powder is mixed by the present invention with solvent, extraction, soaked
Extract.The present invention is not particularly limited to the method for described mixing, using mixed method well-known to those skilled in the art is
Can.In embodiments of the present invention, described mixing makes raw material and solvent mix homogeneously using the method for stirring.
In the present invention, the time of described extraction is 3～5d.Described extraction is preferably carried out under normal temperature condition.
After obtaining lixiviating solution, the present invention is centrifuged to described lixiviating solution, obtains supernatant.In the present invention, described from
The speed of the heart is preferably 2000～4000r/min, more preferably 3500r/min.The time of described centrifugation is 10～20min, more
It is preferably 15min.
After obtaining supernatant, described supernatant is preferably concentrated and is dried by the present invention, obtains floral disc of sunflower and extracts
Thing pressed powder.
In the present invention, described concentration is preferably rotary distillation concentration.The temperature that described rotary distillation concentrates is preferably
30～45 DEG C, more preferably 35～40 DEG C.The time of described concentration is preferably 1～5h, more preferably 2～4h.
In the present invention, described drying is preferably and is vacuum dried.Described vacuum drying condenser temperature is preferably -25
～-55 DEG C, more preferably -30～-45 DEG C.Described vacuum drying vacuum preferably 15～110KPa, more preferably 30
～100KPa.
In the present invention, floral disc of sunflower antibacterial preferably also includes the adjuvant of pesticide field acceptance, makes pesticide field
Dosage form.
The present invention also provides a kind of preparation method of described floral disc of sunflower antibacterial, comprises the following steps：
1) floral disc of sunflower is pulverized, the floral disc of sunflower powder obtaining；
2) described floral disc of sunflower powder is mixed with solvent, extraction, obtain lixiviating solution, described solvent is water, acetic acid second
Ester or petroleum ether；
3) by described step 2) centrifugation of the lixiviating solution that obtains, the supernatant obtaining is floral disc of sunflower extracting solution；
4) by described step 3) adjuvant that received with pesticide field of the floral disc of sunflower extracting solution that obtains mixed, obtains to day
Receptaculum Helianthi antibacterial.
In the present invention, described floral disc of sunflower powder 60～100 mesh sieves excessively before mixing with solvent, more preferably 65～
80 mesh.
In the present invention, the rotating speed of described centrifugation is preferably 2000～4000r/min, more preferably 3500r/min；Described
The time of centrifugation is preferably 10～20min, more preferably 15min.
In the present invention, the suppression of pesticide field made by the adjuvant described floral disc of sunflower extracting solution and pesticide field being accepted
Microbial inoculum dosage form.
In the present invention, the species of the adjuvant that described pesticide field accepts preferably preferably includes wetting agent, dispersant, collapses
Solution agent, stabilizer, pH value regulator, defoamer, one of synergist and filler and several.
In the present invention, the species of described dosage form is preferably water dispersible granules or wettable powder.
Present invention also offers application in inhibition of potato early epidemic pathogenic bacteria for the described floral disc of sunflower antibacterial.
In the present invention, in described floral disc of sunflower antibacterial, the mass concentration of floral disc of sunflower extract is preferably 40
～640mg/ml.
In the present invention, the mass concentration of floral disc of sunflower water extract is preferably 160～640mg/mL in suppression Ma Ling
Application in potato early epidemic pathogenic bacteria, more preferably 320～480mg/mL.
In the present invention, the mass concentration of floral disc of sunflower ethyl acetate extract is preferably 40～360mg/mL in suppression
Application in target bacterium, more preferably 80～240mg/mL.
In the present invention, the mass concentration of floral disc of sunflower ligroin extraction is preferably 40～360mg/mL in suppression horse
Application in bell potato early epidemic pathogenic bacteria, 80～240mg/mL.
The floral disc of sunflower antibacterial that the present invention provided with reference to embodiment, preparation method and its in preventing and treating Rhizoma Solani tuber osi
Application in early blight is described in detail, but they can not be interpreted as limiting the scope of the present invention.
Embodiment 1
The floral disc of sunflower select no insect pest, going mouldy is raw material, makes powdery with pulverizer, crosses 60 mesh sieves, and collection obtains
Floral disc of sunflower powder.Weigh powder 1kg, mix with the water of 10L, add in bucket and extract 5 days, lixiviating solution is with 3000r/min
Rotating speed is centrifuged 15min, collects supernatant, concentrated by rotary evaporation 2h under conditions of 45 DEG C, concentrate, through carrying out vacuum lyophilization, obtains
Extract powder, in 4 DEG C of refrigerators, preservation is standby.
Embodiment 2
The floral disc of sunflower select no insect pest, going mouldy is raw material, makes powdery with pulverizer, crosses 80 mesh sieves, and collection obtains
Floral disc of sunflower powder.Weigh powder 1kg, mix with the water of 15L, add in bucket and extract 3 days, lixiviating solution is with 3500r/min
Rotating speed is centrifuged 12min, collects supernatant, concentrated by rotary evaporation 3h under conditions of 35 DEG C, concentrate, through vacuum lyophilization, obtains extraction
Thing powder, in 4 DEG C of refrigerators, preservation is standby.
Embodiment 3
The floral disc of sunflower select no insect pest, going mouldy is raw material, makes powdery with pulverizer, crosses 100 mesh sieves, and collection obtains
Floral disc of sunflower powder.Weigh powder 1kg, mix with the ethyl acetate of 5L, add bucket in extract 4 days, lixiviating solution with
4000r/min rotating speed is centrifuged 10min, collects supernatant, concentrated by rotary evaporation 4h under conditions of 30 DEG C, concentrate is done through vacuum freezing
Dry, obtain extract powder, in 4 DEG C of refrigerators, preservation is standby.
Embodiment 4
The floral disc of sunflower select no insect pest, going mouldy is raw material, makes powdery with pulverizer, crosses 80 mesh sieves, and collection obtains
Floral disc of sunflower powder.Weigh powder 1kg, mix with the ethyl acetate of 20L, add bucket in extract 5 days, lixiviating solution with
3500r/min rotating speed is centrifuged 15min, collects supernatant, concentrated by rotary evaporation 5h under conditions of 37 DEG C, concentrate is done through vacuum freezing
Dry, obtain extract powder, in 4 DEG C of refrigerators, preservation is standby.
Embodiment 5
The floral disc of sunflower select no insect pest, going mouldy is raw material, makes powdery with pulverizer, crosses 90 mesh sieves, and collection obtains
Floral disc of sunflower powder.Weigh powder 1kg, mix with the petroleum ether of 10L, add in bucket and extract 3 days, lixiviating solution is with 3000r/
Min rotating speed is centrifuged 20min, collects supernatant, concentrated by rotary evaporation 1h under conditions of 40 DEG C, concentrate, through vacuum lyophilization, obtains
Extract powder, in 4 DEG C of refrigerators, preservation is standby.
Embodiment 6
The floral disc of sunflower select no insect pest, going mouldy is raw material, makes powdery with pulverizer, crosses 70 mesh sieves, and collection obtains
Floral disc of sunflower powder.Weigh powder 1kg, mix with the petroleum ether of 20L, add in bucket and extract 5 days, lixiviating solution is with 4000r/
Min rotating speed is centrifuged 10min, collects supernatant, concentrated by rotary evaporation 4h under conditions of 35 DEG C, concentrate, through vacuum lyophilization, obtains
Extract powder, in 4 DEG C of refrigerators, preservation is standby.
Embodiment 7 and 8
The floral disc of sunflower water extract powder that embodiment 1 and 2 is obtained be configured to respectively mass concentration be 160mg/mL,
320mg/mL, 480mg/mL and 640mg/mL.
Floral disc of sunflower water extract bacteriostatic test prepares 4 culture dishs of PDA containing 20mL, after PDA solidification, dense by 9
The extracting solution of degree is respectively dropped into 1mL and and is coated with uniformly as test group to different culture dishs, and the culture dish not dealt with is sky
White comparison.Each culture dish is inverted equidistant 3 cultures diameter 6mm of 1 week that accesses mutually every Alternariaspp cake.After inoculation, own
24h cultivated in 28 DEG C of constant incubators by culture dish.Culture terminate after, with slide gauge with crossing method survey calculation each
The meansigma methodss of 3 colony diameters in culture dish, and bacteriostasis rate is calculated according to colony diameter.
The floral disc of sunflower water extract that embodiment 1 and embodiment 2 obtain is to being mutually shown in Table 1 every the inhibitory action of interlinkage spore.
Table 1 floral disc of sunflower water extract variable concentrations are to mutually every the size of the inhibition zone of the generation of interlinkage spore
Embodiment 9 and 10
It is 40mg/ that the floral disc of sunflower ethyl acetate extract powder that embodiment 3 and 4 is obtained is configured to mass concentration respectively
ML, 80mg/mL, 240mg/mL and 360mg/mL.
Floral disc of sunflower ethyl acetate extract prepares 4 culture dishs of PDA containing 20mL, after PDA solidification, by 4 concentration
Extracting solution be respectively dropped into 1mL and and be coated with uniformly as test group to different culture dishs, the culture dish not dealt with is blank
Comparison, identical with water position bacteriostatic test with the method such as embodiment 7 and the inoculation of 8 identical methods, culture, observation, measurement.
The floral disc of sunflower ethyl acetate extract that embodiment 3 and embodiment 4 obtain is to mutually every the inhibitory action of interlinkage spore
It is shown in Table 2.
The size to the inhibition zone mutually producing every interlinkage spore for the table 2 floral disc of sunflower ethyl acetate extract variable concentrations
Embodiment 11 and 12
It is 40mg/ that the floral disc of sunflower petroleum ether extract powder that embodiment 5 and 6 is obtained is configured to mass concentration respectively
ML, 80mg/mL, 240mg/mL and 360mg/mL.
Floral disc of sunflower ligroin extraction prepares 4 culture dishs of PDA containing 20mL, after PDA solidification, by 4 concentration
Extracting solution is respectively dropped into 1mL and and is coated with uniformly as test group to different culture dishs, and the culture dish not dealt with is blank right
According to identical with water position bacteriostatic test with the method such as embodiment 7 and the inoculation of 8 identical methods, culture, observation, measurement.
The floral disc of sunflower ligroin extraction that embodiment 5 and embodiment 6 obtain is to being mutually shown in every the inhibitory action of interlinkage spore
Table 3.
The size to the inhibition zone mutually producing every interlinkage spore for the table 3 floral disc of sunflower ligroin extraction variable concentrations
As seen from the above embodiment, the invention provides floral disc of sunflower antibacterial, including with floral disc of sunflower as raw material
The floral disc of sunflower extract being obtained using solvent extraction, described solvent is water, ethyl acetate or petroleum ether.Using opposed polarity
Solvent extraction floral disc of sunflower in opposed polarity active substance, gained water, ethyl acetate or Petroleum ether extraction obtain
The active substance of floral disc of sunflower all has bacteria resistance function.
The above is only the preferred embodiment of the present invention it is noted that ordinary skill people for the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. floral disc of sunflower antibacterial, adopts the floral disc of sunflower that solvent extraction obtains to extract including with floral disc of sunflower for raw material
Thing, described solvent is water, ethyl acetate or petroleum ether.
2. floral disc of sunflower antibacterial according to claim 1 it is characterised in that the quality of described floral disc of sunflower with molten
The volume ratio of agent is 1kg：(5～15) L.
3. floral disc of sunflower antibacterial according to claim 1 and 2 it is characterised in that the method for described extraction include with
Lower step：
1) floral disc of sunflower is pulverized, the floral disc of sunflower powder obtaining；
2) described floral disc of sunflower powder is mixed with solvent, extraction, obtain lixiviating solution；
3) by described step 2) centrifugation of the lixiviating solution that obtains, the supernatant obtaining is floral disc of sunflower extract.
4. floral disc of sunflower antibacterial according to claim 3 is it is characterised in that the time of described extraction is 3～5d.
5. floral disc of sunflower antibacterial according to claim 3 is it is characterised in that described step 3) in centrifugation after also wrap
Include：The supernatant obtaining is concentrated and is dried.
6. the floral disc of sunflower antibacterial described in Claims 1 to 5 any one preparation method it is characterised in that include with
Lower step：
1) floral disc of sunflower is pulverized, the floral disc of sunflower powder obtaining；
2) described floral disc of sunflower powder is mixed with solvent, extraction, obtain lixiviating solution, described solvent be water, ethyl acetate or
Petroleum ether；
3) by described step 2) centrifugation of the lixiviating solution that obtains, the supernatant obtaining is floral disc of sunflower extracting solution；
4) by described step 3) adjuvant that received with pesticide field of the floral disc of sunflower extracting solution that obtains mixed, and obtains Flos Helianthi
Disk antibacterial.
7. preparation method according to claim 6 is it is characterised in that described step 2) in floral disc of sunflower powder with
60～100 mesh sieves are crossed before solvent mixing.
8. preparation method according to claim 6 is it is characterised in that described step 3) in centrifugation rotating speed be 2000～
4000r/min rotating speed；Described step 3) in centrifugation time be 10～20min.
9. application in inhibition of potato early epidemic pathogenic bacteria for the floral disc of sunflower antibacterial described in Claims 1 to 5 any one.
10. the application according to claim 9 is it is characterised in that Flos Helianthi in described floral disc of sunflower antibacterial
The concentration of disk extract is 40～640mg/ml.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610779488.1A CN106376606B (en) | 2016-08-31 | 2016-08-31 | Floral disc of sunflower bacteriostatic agent, preparation method and its application in prevention and treatment target |
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| CN201610779488.1A CN106376606B (en) | 2016-08-31 | 2016-08-31 | Floral disc of sunflower bacteriostatic agent, preparation method and its application in prevention and treatment target |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117919291A (en) * | 2024-01-26 | 2024-04-26 | 吉林农业大学 | Application of sunflower disc extract in preparation of antibacterial products |
Citations (2)
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|---|---|---|---|---|
| CN1307809A (en) * | 2001-02-28 | 2001-08-15 | 高福兴 | Preparation of green pesticide |
| CN102924622A (en) * | 2012-11-19 | 2013-02-13 | 温雪 | Helianthus annuus polysaccharide and preparation method thereof |
-
2016
- 2016-08-31 CN CN201610779488.1A patent/CN106376606B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1307809A (en) * | 2001-02-28 | 2001-08-15 | 高福兴 | Preparation of green pesticide |
| CN102924622A (en) * | 2012-11-19 | 2013-02-13 | 温雪 | Helianthus annuus polysaccharide and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 杨树平等: ""向日葵盘总黄酮工艺优化及含量测定"", 《食品科学》 * |
| 索金玲: ""向日葵花盘中有效化学成分分析及抗氧化性测定"", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117919291A (en) * | 2024-01-26 | 2024-04-26 | 吉林农业大学 | Application of sunflower disc extract in preparation of antibacterial products |
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