CN106367362B - 一种去除六价铬污染的链霉菌及其应用 - Google Patents

一种去除六价铬污染的链霉菌及其应用 Download PDF

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CN106367362B
CN106367362B CN201610077641.6A CN201610077641A CN106367362B CN 106367362 B CN106367362 B CN 106367362B CN 201610077641 A CN201610077641 A CN 201610077641A CN 106367362 B CN106367362 B CN 106367362B
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hexavalent chromium
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孙莹
赵英
王新民
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Abstract

本发明公开了一种高效去除六价铬污染的链霉菌Streptomyces puniceus strain.LYzhlm，该菌种于2015年12月2日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏编号为CGMCC No.11773，命名为Streptomyces puniceus strain.LYzhlm。本发明的有益效果：具有较强的去除六价铬性能，在有氧、中温的条件下，能对浓度为100mg/L～1000mg/L的六价铬离子还原到0，还原率为100％。

Description

一种去除六价铬污染的链霉菌及其应用
技术领域
本发明涉及微生物技术领域，尤其涉及一种去除六价铬污染的链霉菌。
背景技术
随着社会和经济的快速发展，铬在工业中得到了大量应用，但是与此同时也给环境带来了大量污染。在工业中，铬被广泛应用于电镀、木材防腐、皮革制造、钢铁等行业，最终以废水、废渣等形式排放到环境中去。国家环保部的资料显示，目前，我国受铬等重金属污染的耕地面积近2000万公顷，约占耕地总面积的1/5。全国每年因重金属污染而减产粮食一千多万吨。铬虽然是人和动物所必需的微量元素，但是过量的铬有致癌作用，尤其是六价铬是一种危害极大的致癌物质。铬污染不仅已经成为危害环境，影响人类健康的元凶之一，同时也严重损伤农作物机体。
目前，化学还原法是常用的铬污染修复方法，但存在处理效果较差、成本高、能耗大，以及会产生二次污染等问题。传统的铬污染治理方法有碱沉淀、膜分离、离子交换和吸附等，前者在治理过程中会产生大量的污泥，后两者则由于治理成本高而受限，由于各方法都存在缺陷，至今还未找到有效的综合利用方式。
自从上世纪七十年代被发现微生物具有六价铬还原能力以来，Romanenko等在1977年首次报道脱色杆菌能够在厌氧的条件下还原六价铬。研究人员发现了越来越多的能够对六价铬进行还原的微生物，并对其展开了大量研究，为后期在实践中的广泛应用奠定了坚实的基础。由于生物处理技术具有二次污染小、价格低廉、经济有效等优点，已成为一种修复六价铬环境污染的新兴友好代替技术。从六价铬污染水体或土壤中分离微生物，并有针对地将其应用于污染水体及土壤的修复治理过程中，是一种较为理想的修复方法。
因此研究开发生物修复六价铬环境污染的菌株具有广阔的应用空间。
发明内容
本发明针对现有技术存在的不足，提供一种去除六价铬污染的链霉菌。
本发明技术方案如下：
一种去除六价铬污染的链霉菌Streptomyces puniceus strain.LYzhlm，该菌种于2015年12月2日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏编号为CGMCC No.11773，命名为Streptomyces puniceus strain.LYzhlm。地址：北京市朝阳区北辰西路中科院微生物研究所。
其16S rDNA序列是序列表SEQ NO.1的序列。
该去除六价铬污染的链霉菌应用在去除六价铬污染方面。
LYzhlm菌株来自于工业污泥，经过室内驯化、分离和纯化，筛选出来。LYzhlm菌株在LB培养基上培养，细胞形态为丝状；培养4天，菌落颜色为灰白色，菌落形态为圆形、不光滑。基内菌丝不断裂，气生菌丝通常发育良好，形成长的孢子丝。基内菌丝多分枝，产生紫红色水溶性色素。显微观察孢子丝线形。孢子椭圆形，表面光滑。
铬在土壤中最常见的价态为三价铬和六价铬，在自然环境中，六价铬容易被微生物还原为三价铬，而三价铬则难以被氧化为六价铬。土壤中六价铬通常以铬酸根(CrO4 2-)和重铬酸根(Cr2O4 2-)的形式存在，活性较高，对生物体毒害作用较强。相对于六价铬来讲，三价铬在土壤中主要以Cr(H2O)6 3+、Cr(H2O)2+、CrO2+、形式存在，容易被土壤吸附或生成沉淀，对生物体毒害作用较低，对环境影响相对较小。经筛选驯化所得的紫红链霉菌(Streptomyces puniceus strain LYzhlm)改变重金属的化学形态，使重金属固定，生物可利用性降低，减少重金属的危害。本技术与物理、化学修复技术相比，使用LYzhlm修复技术由于其安全性、非破坏性和经济性的优势，为治理六价铬高污染提供一种菌株。
利用细菌16S rDNA通用引物，用PCR的方法扩增的LYzhlm菌株。16S rDNA部分片段，PCR产物测序获得该片段序列，测序所LYzhlm菌株的16S rDNA(长度1.4kbp)结果如序列表SEQ NO.1。
将序列表SEQ NO.1，应用BLAST工具进行基因同源性和相似性分析，经Blast检索，可知LYzhlm菌株的16S rDNA序列与紫金链霉菌的同源性最高，达到99％(见表1)。
表1 BLAST检索结果表
根据BLAST分析结果，菌株所属生物分类学信息如下：
domain：Bacteria；
phylum：Actinobacteria；
class：Streptomycetales；
order：Streptomycetaceae；
family：Streptomyces；
Genus：Streptomyces puniceus。
因此，将该新菌株鉴定为紫红链霉菌(Streptomyces puniceus strain)，并命名为紫红链霉菌(Streptomyces puniceus strain LYzhlm)。
本发明的有益效果：
本发明的紫红链霉菌(Streptomyces puniceus strain LYzhlm)菌株具有较强的去除六价铬性能，在有氧、中温的条件下，能对浓度为100mg/L～1000mg/L的六价铬离子还原到0，还原率为100％。
附图说明
图1为LYzhlm处理六价铬废水六价铬离子去除曲线。
具体实施方式
实施例1.检测依据，污水中六价铬测定方法：
GB-T 7467-87水质六价铬的测定二苯碳酰二肼分光光度法
实施例2用本菌株处理六价铬重金属的试验如下：
1.试验方法
①菌株培养基制备：酵母粉0.05％,蛋白胨0.1％，氯化钠0.05％，葡萄糖0.05％，调节PH值为7.2,121℃灭菌30分钟。固体培养基：在上述培养基中加2％的琼脂。
②菌株发酵液制备：将菌株3环接种到100ml培养基中，摇床培养72小时，培养温度30℃，摇床转速100(转/分)。
③将重镉酸钾加入上述方法生产的发酵液中，使六价铬浓度分别达到100mg/L、250mg/L、500mg/L、750mg/L、1000mg/L，控制废水温度为30℃，搅拌转速为100r/min，的条件下处理间隔24h取样，测定六价铬的浓度变化。培养基中Cr(Ⅵ)浓度低于检出限后停止取样。
2.试验结果
试验结果见表1和图1。
表1 LYzhlm处理六价铬废水实验结果表
试验表明，六价铬污染水平为100～1000mg/L时，LYzhlm发酵液均能使六价铬离子含量降低到0，去除率达100％。
上列详细说明是针对本发明可行实施例的具体说明，该实施例并非用以限制本发明的专利范围，凡未脱离本发明所为的等效实施或变更，均应包含于本案的专利范围中。
<110>路域生态工程有限公司
<120>一种高效去除六价铬污染的链霉菌及其应用
<160>1
<210> 1
<211> 1431
<212> DNA
<213> 人工序列
<400> 1
CTTACCTAACCGCGCTTACCATGCAGTCGACGATGAACCGCTTCGGTGGTGGATTAGTGGCGAACGGGTGAGTAACACGTGGGCAATCTGCCCTTCACTCTGGGACAAGCCCTGGAAACGGGGTCTAATACCGGATAATACTCCTGCCTGCATGGGTGGGGGTTGAAAGCTCCGGCGGTGAAGGATGAGCCCGCGGCCTATCAGCTTGTTGGTGGGGTAATGGCCTACCAAGGCGACGACGGGTAGCCGGCCTGAGAGGGCGACCGGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGATGCAGCGACGCCGCGTGAGGGATGACGGCCTTCGGGTTGTAAACCTCTTTCAGCAGGGAAGAAGCGCAAGTGACGGTACCTGCAGAAGAAGCGCCGGCTAACTACGTGCCAGCAGCCGCGGTAATACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTAGGCGGCTTGTCACGTCGGATGTGAAAGCCCGGGGCTTAACCCCGGGTCTGCATTCGATACGGGCTAGCTAGAGTGTGGTAGGGGAGATCGGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGGTGGCGAAGGCGGATCTCTGGGCCATTACTGACGCTGAGGAGCGAAAGCGTGGGGAGCGAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGTTGGGAACTAGGTGTTGGCGACATTCCACGTCGTCGGTGCCGCAGCTAACGCATTAAGTTCCCCGCCTGGGGAGTACGGCCGCAAGGCTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCAGCGGAGCATGTGGCTTAATTCGACGCAACGCGAAGAACCTTACCAAGGCTTGACATATACCGGAAAGCATCAGAGATGGTGCCCCCCTTGTGGTCGGTATACAGGTGGTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGTTCTGTGTTGCCAGCATGCCTTTCGGGGTGATGGGGACTCACAGGAGACTGCCGGGGTCAACTCGGAGGAAGGTGGGGACGACGTCAAGTCATCATGCCCCTTATGTCTTGGGCTGCACACGTGCTACAATGGCCGGTACAATGAGCTGCGATGCCGTGAGGCGGAGCGAATCTCAAAAAGCCGGTCTCAGTTCGGATTGGGGTCTGCAACTCGACCCCATGAAGTCGGAGTTGCTAGTAATCGCAGATCAGCATTGCTGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACGTCACGAAAGTCGGTAACACCCGAAGCCGGTGGCCCAACCCCTGTGGGAGGAGCTTCGAAGTGACTTCTTGGGGTC

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1.一种去除六价铬污染的链霉菌(Streptomyces puniceus strain.)LYzhlm，其特征在于：该菌种于2015年12月2日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏编号为CGMCC No.11773，命名为Streptomyces puniceus strain.Lyzhlm；其16S rDNA序列是序列表SEQ NO.1的序列。
CN201610077641.6A 2016-02-03 2016-02-03 一种去除六价铬污染的链霉菌及其应用 Expired - Fee Related CN106367362B (zh)

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CN103205384A (zh) * 2013-04-17 2013-07-17 广州利万世环保科技有限公司 一种土壤重金属六价铬生物降解剂及其制造方法

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