CN106361785A - Sandalwood oil extract and application thereof - Google Patents
Sandalwood oil extract and application thereof Download PDFInfo
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- CN106361785A CN106361785A CN201610778995.3A CN201610778995A CN106361785A CN 106361785 A CN106361785 A CN 106361785A CN 201610778995 A CN201610778995 A CN 201610778995A CN 106361785 A CN106361785 A CN 106361785A
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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Abstract
The invention discloses a sandalwood oil extract and application thereof. According to gas chromatography of the sandalwood oil extract, characteristic peaks exist at the positions where the retention times are 19.434, 24.179, 25.197, 27.762, 29.531 and 29.902, wherein the unit of the retention times is minute, and the difference of the retention times is +/-5%. The sandalwood oil extract has the advantage that the effects can be quickly achieved when the sandalwood oil extract is used for treating or relieving diseases such as angina pectoris.
Description
Technical field
The present invention relates to a kind of Lignum Santali Albi oil extract and application thereof.
Background technology
The chest stuffiness relieving aerosol is the important drugs of allevating angina pectoris, its Main Ingredients and Appearance be Oleum Asari, Oleum Santali albi, Rhizoma Alpiniae Officinarum oil,
Fructus Piperis Longi oil and Borneolum Syntheticum.The main component of the chest stuffiness relieving aerosol is volatile oil, can quickly be absorbed by the body into blood, coronary artery dilating blood
Pipe, significantly improves myocardial ischemia, rapid recovery is uncomfortable in chest, chest pain, cardiopalmus, shortness of breath, the angina pectoris symptom such as weak.Wherein, Oleum Santali albi
It is wherein very important composition.
With regard to Oleum Santali albi, current research is concentrated mainly in Oleum Santali albi application enhancements.For example, cn1732908 a is open
A kind of spray containing Borneolum Syntheticum, in its medicinal liquid containing 0.01～20% Borneolum Syntheticum, 5～40% ethanol and neutral fat
Oil, can also contain the pharmacological component such as Oleum Asari, Oleum Santali albi, Rhizoma Alpiniae Officinarum oil, Fructus Piperis Longi oil.The stimulation to mucosa for this spray
Property is little.And for example, cn103796650a discloses the compositionss of Lignum Santali Albi heartwood oil, include the Lignum Santali Albi heartwood oil of the upper effective dose for the treatment of with
Lignum Santali Albi fruit oil.Said composition can be used for treating dermatosiss.For another example, cn103638546a disclose one kind calm the nerves with fresh air
Agent, including Oleum Santali albi, Oleum Rosae Rugosae, Cananga odorata oil, Oleum lavandula angustifolia, ethanol, water.The calmness that has of this air freshener is allayed excitement and is dropped hot-tempered
The function of refreshment.
It is known that the quality of Chinese medicinal herbses is directly connected to the quality of medicine.However, high-quality germ plasm resource is relatively deficient
Weary, quality of medicinal material is very different.Therefore, how to determine that the high Lignum Santali Albi oil extract of good effect, oil content just seems particularly significant.
But, current Lignum Santali Albi crude drug source mixes, and this leads to the effective ingredient of Lignum Santali Albi oil extract to there is many differences, thus leading to it
Drug effect is significantly different.This for a kind of medicine needing quick acting it is critical that.
Therefore, in the urgent need to a kind of Lignum Santali Albi extract, it has good medicine for diseases such as treatment or allevating angina pectoris
Effect.
Content of the invention
It is an object of the present invention to provide a kind of Lignum Santali Albi extract, it is for the diseases such as treatment or allevating angina pectoris tool
There is quick acting.Further object is that providing the detection method of above-mentioned Lignum Santali Albi oil extract, Ke Yirong
Easily differentiate rapid-action Lignum Santali Albi oil extract.It is yet a further object of the present invention to provide comprising the medicine group of this Lignum Santali Albi extract
Compound.It is also an object of the present invention to provide the purposes of this Lignum Santali Albi extract.
Present inventor has made intensive studies, and finds that following technical scheme can realize above-mentioned purpose.
The present invention provides a kind of Lignum Santali Albi oil extract, and the gas chromatogram of this Lignum Santali Albi oil extract includes following characteristic peak:
| Retention time | Peak area |
| 19.434 | 1.8～6.0%, |
| 24.179 | 3.0～5.0%, |
| 25.197 | 1.0～5.0%, |
| 27.762 | 20.0～35.0%, |
| 29.531 | 1.0～2.0%, and |
| 29.902 | 1.8～6.0%； |
Wherein, the unit of described retention time is minute, and its deviation is ± 5%；Described peak area is with this Lignum Santali Albi oil extract
Calculated on the basis of the overall peak area of the gas chromatogram of thing.
Lignum Santali Albi oil extract according to the present invention is it is preferable that described retention time and peak area are as follows:
| Retention time | Peak area |
| 19.434 | 2.0～4.5%, |
| 24.179 | 3.2～3.9%, |
| 25.197 | 1.3～4.5%, |
| 27.762 | 25.0～30.5%, |
| 29.531 | 1.3～1.7%, and |
| 29.902 | 2.0～5.2%； |
Lignum Santali Albi oil extract according to the present invention is it is preferable that the gas chromatogram of this Lignum Santali Albi oil extract also includes following spy
Levy peak:
| Retention time | Peak area |
| 19.655 | 1.8～15.0%, |
| 24.710 | 2.2～10.0%, and |
| 27.298 | 10.0～35.0%； |
Wherein, the unit of described retention time is minute, and its deviation is ± 5%；Described peak area is with this Lignum Santali Albi oil extract
Calculated on the basis of the overall peak area of the gas chromatogram of thing.
Lignum Santali Albi oil extract according to the present invention is it is preferable that the gas chromatogram of this Lignum Santali Albi oil extract also includes following spy
Levy peak:
Wherein, the unit of described retention time is minute, and its deviation is ± 5%；Described peak area is with this Lignum Santali Albi oil extract
Calculated on the basis of the overall peak area of the gas chromatogram of thing.
Lignum Santali Albi oil extract according to the present invention is it is preferable that the characteristic peak of the gas chromatogram of this Lignum Santali Albi oil extract is selected from
One of following two stack features peaks:
(1) first stack features peak:
| Retention time | Peak area |
| 19.434 | 2.07514%, |
| 19.655 | 2.10358%, |
| 24.179 | 3.20674%, |
| 24.710 | 9.49627%, |
| 25.197 | 1.34512%, |
| 27.298 | 16.05219%, |
| 27.762 | 30.13516%, |
| 29.531 | 1.43918%, and |
| 29.902 | 2.23520%； |
(2) second stack features peaks:
Wherein, the unit of described retention time is minute, and its deviation is ± 5%；Described peak area is with this Lignum Santali Albi oil extract
Calculated on the basis of the overall peak area of the gas chromatogram of thing, its deviation is ± 3%.
Lignum Santali Albi oil extract according to the present invention is it is preferable that described gas chromatogram obtains by the following method:
(1) Oleum Santali albi is adopted 8～10 times of diluted ethyl acetate；
(2) using the quartzy capillary that length is 30m, internal diameter is 0.25mm and fixing phase coating thickness of liquid film is 0.25 μm
Pipe, sampling condition is as follows:
Injector temperature is 250 DEG C, 250 DEG C of fid detection temperature, and carrier gas is helium, and flow velocity is 1.0ml/min, split ratio
For 40:1, sample size is 0.2 μ l；
Heating schedule is as follows:
Column temperature is 90 DEG C, is warming up to 160 DEG C with the heating rate of 5 DEG C/min, then is heated up with the heating rate of 1 DEG C/min
166 DEG C, then it is incubated 5min, then is heated up 167 DEG C with the heating rate of 0.5 DEG C/min, be incubated 5min, then with 0.5 DEG C/min's
Heating rate heats up 168 DEG C, is incubated 2min, is finally warming up to 230 DEG C with the heating rate of 10 DEG C/min.
The present invention also provides the detection method of above-mentioned Lignum Santali Albi oil extract, and described method comprises the steps:
(1) Oleum Santali albi is adopted 8～10 times of diluted ethyl acetate；
(2) using the quartz capillary that length is 30m, internal diameter is 0.25mm and fixing phase coating thickness of liquid film is 0.25 μm
Post, sampling condition is as follows:
Injector temperature is 250 DEG C, 250 DEG C of fid detector temperature, and carrier gas is helium, and flow velocity is 1.0ml/min, shunting
For 40:1, sample size is 0.2 μ l to ratio；
Heating schedule is as follows:
Column temperature is 90 DEG C, is warming up to 160 DEG C with the heating rate of 5 DEG C/min, then is heated up with the heating rate of 1 DEG C/min
166 DEG C, then it is incubated 5min, then is heated up 167 DEG C with the heating rate of 0.5 DEG C/min, be incubated 5min, then with 0.5 DEG C/min's
Heating rate heats up 168 DEG C, is incubated 2min, is finally warming up to 230 DEG C with the heating rate of 10 DEG C/min.
Further, the present invention provides a kind of pharmaceutical composition for treatment or allevating angina pectoris, this pharmaceutical composition
Comprise above-mentioned Lignum Santali Albi oil extract.
Pharmaceutical composition according to the present invention is it is preferable that this pharmaceutical composition also includes Oleum Asari, Rhizoma Alpiniae Officinarum oil, Fructus Piperis Longi
Oil, Borneolum Syntheticum and pharmaceutically acceptable adjuvant.
Further, the present invention also provides above-mentioned Lignum Santali Albi oil extract in the pharmaceutical preparation of preparation treatment or allevating angina pectoris
In purposes.
The Lignum Santali Albi oil extract of the present invention has specific characteristic peak in gas chromatogram, so that comprising this Oleum Santali albi
The chest stuffiness relieving aerosol of extract has the characteristics that quick acting for treatment or allevating angina pectoris.The present invention adopts gas chromatography
Separate Lignum Santali Albi oil extract, each component of Lignum Santali Albi oil extract, high resolution can be detected well, easily differentiate quick acting
Lignum Santali Albi oil extract.
Brief description
Fig. 1 is the gas chromatogram of Oleum Santali albi c.
Specific embodiment
With reference to specific embodiment, the present invention is further illustrated, but protection scope of the present invention is not limited to
This.
In the present invention, the unit of the retention time of the characteristic peak of each component is minute, and its deviation is ± 5%, preferably
For ± 3%, more preferably ± 1%.Described peak area is on the basis of the overall peak area of the gas chromatogram of this Lignum Santali Albi oil extract
Calculated, its deviation is ± 3%, preferably ± 1%.So-called " overall peak area ", refers to the gas phase color of Lignum Santali Albi oil extract
The area sum of all characteristic peaks under spectral curve, it does not include the characteristic peak area of reference substance.
<Lignum Santali Albi oil extract>
The Lignum Santali Albi oil extract of the present invention can be obtained using conventional method.Preferably, the Lignum Santali Albi oil extract of the present invention
Thing obtains by the following method: weighs 100.0g Lignum Santali Albi medical material, wood shavings, soaks 12h, extracts 5h through vapor distillation, take upper strata
Volatile oil is dehydrated with anhydrous sodium sulfate, filters, as Lignum Santali Albi oil extract, lucifuge, sealing preserve, standby.
The Lignum Santali Albi oil extract of the present invention is obtained using the Lignum Santali Albi medicinal material extract of the different places of production or different batches.But
It is that the gas chromatogram of this Lignum Santali Albi oil extract must is fulfilled for following condition, that is, it includes following characteristic peak:
In the present invention, the gas chromatogram of this Lignum Santali Albi oil extract preferably also includes the following features peak:
According to a specific embodiment of the present invention, the characteristic peak of the gas chromatogram of this Lignum Santali Albi oil extract is first group
Characteristic peak:
| Retention time | Peak area |
| 19.434 | 2.07514%, |
| 19.655 | 2.10358%, |
| 24.179 | 3.20674%, |
| 24.710 | 9.49627%, |
| 25.197 | 1.34512%, |
| 27.298 | 16.05219%, |
| 27.762 | 30.13516%, |
| 29.531 | 1.43918%, and |
| 29.902 | 2.23520%. |
According to the another embodiment of the present invention, the characteristic peak of the gas chromatogram of this Lignum Santali Albi oil extract is second
Stack features peak:
In the present invention, above-mentioned gas chromatogram can obtain by the following method:
(1) Oleum Santali albi is adopted 8～10 times of diluted ethyl acetate；For example by 0.5ml Oleum Santali albi with diluted ethyl acetate extremely
5ml；
(2) with length as 30m, internal diameter is as 0.25mm with quartz capillary column that fixing phase coating thickness of liquid film is 0.25 μm
Sample introduction, wherein, sampling condition is as follows:
Injector temperature is 250 DEG C, and fid (flame ion) detector is 250 DEG C, and carrier gas is helium, and flow velocity is
1.0ml/min, split ratio is 40:1, and sample size is 0.2 μ l；
Heating schedule is as follows:
Column temperature is 90 DEG C, is warming up to 160 DEG C with the heating rate of 5 DEG C/min, then is heated up with the heating rate of 1 DEG C/min
166 DEG C, then it is incubated 5min, then is heated up 167 DEG C with the heating rate of 0.5 DEG C/min, be incubated 5min, then with 0.5 DEG C/min's
Heating rate heats up 168 DEG C, is incubated 2min, is finally warming up to 230 DEG C with the heating rate of 10 DEG C/min.
The quartz capillary column of the present invention can adopt the hp-5 quartz capillary column (30m × 0.25mm of Agilent company
×0.25μm).
Those skilled in the art all know, above-mentioned parameter only one reference value is it is allowed to have rational deviation and change.
<detection method>
The present invention also provides the detection method of above-mentioned Lignum Santali Albi oil extract.This detection method may include steps of:
(1) Oleum Santali albi is adopted 8～10 times of diluted ethyl acetate；For example by 0.5ml Oleum Santali albi with diluted ethyl acetate extremely
5ml；
(2) using the quartz capillary that length is 30m, internal diameter is 0.25mm and fixing phase coating thickness of liquid film is 0.25 μm
Post, wherein, sampling condition is as follows:
Injector temperature is 250 DEG C, 250 DEG C of fid (flame ion) detector temperature, and carrier gas is helium, and flow velocity is
1.0ml/min, split ratio is 40:1, and sample size is 0.2 μ l；
Heating schedule is as follows:
Column temperature is 90 DEG C, is warming up to 160 DEG C with the heating rate of 5 DEG C/min, then is heated up with the heating rate of 1 DEG C/min
166 DEG C, then it is incubated 5min, then is heated up 167 DEG C with the heating rate of 0.5 DEG C/min, be incubated 5min, then with 0.5 DEG C/min's
Heating rate heats up 168 DEG C, is incubated 2min, is finally warming up to 230 DEG C with the heating rate of 10 DEG C/min.
In the detection method of the present invention, this quartz capillary column can adopt the hp-5 quartz capillary of Agilent company
Post (30m × 0.25mm × 0.25 μm).
In the detection method of the present invention, the specific descriptions of above-mentioned Lignum Santali Albi oil extract are referring to<Lignum Santali Albi oil extract>portion
Point, repeat no more here.
Those skilled in the art all know, above-mentioned parameter only one reference value is it is allowed to have rational deviation and change.
<pharmaceutical composition and purposes>
The pharmaceutical composition of the present invention comprises above-mentioned Lignum Santali Albi oil extract.This pharmaceutical composition for treatment or can be alleviated
Angina pectoriss have the characteristics that rapid-action.The specific descriptions of above-mentioned Lignum Santali Albi oil extract referring to<Lignum Santali Albi oil extract>part, here
Repeat no more.
In the present invention, this pharmaceutical composition can also include Oleum Asari, Rhizoma Alpiniae Officinarum oil, Fructus Piperis Longi oil, Borneolum Syntheticum and pharmacy
Upper acceptable adjuvant.With Oleum Santali albi for 70 parts by volume as standard, Oleum Asari is 20～30 parts by volume；Rhizoma Alpiniae Officinarum oil is 30～35
Parts by volume, Fructus Piperis Longi oil is 10～20 parts by volume；Borneolum Syntheticum is 20～25 weight portions.According to a specific embodiment of the present invention,
This pharmaceutical composition includes 23ml Oleum Asari, 70ml Oleum Santali albi, 32ml Rhizoma Alpiniae Officinarum oil, 15ml Fructus Piperis Longi oil and 22.5g Borneolum Syntheticum.Pharmacy
Upper acceptable adjuvant can be solvent, propellant etc..Solvent can be dehydrated alcohol；Propellant can be 1,1,1,2- tetrafluoro
Ethane.
The present invention provides purposes in the pharmaceutical preparation of preparation treatment or allevating angina pectoris for the above-mentioned Lignum Santali Albi oil extract.Should
Oleum Asari, Rhizoma Alpiniae Officinarum oil, Fructus Piperis Longi oil, Borneolum Syntheticum and pharmaceutically acceptable adjuvant can also be contained in pharmaceutical preparation.Oleum Asari,
Rhizoma Alpiniae Officinarum oil, Fructus Piperis Longi oil, the consumption of Borneolum Syntheticum, the specific descriptions of the species of pharmaceutically acceptable adjuvant are as it was previously stated, here not
Repeat again.
Embodiment
Weigh 100.0g Lignum Santali Albi medical material, wood shavings, soak 12h, extract 5h through vapor distillation, take upper strata volatile oil with anhydrous
Sodium sulfate is dehydrated, filter, both Lignum Santali Albi oil extract, lucifuge, sealing preserve, standby.
By above-mentioned volatile oil component through gas chromatographic detection, select retention time to be 19.434 respectively, 19.655,
24.179th, 24.710,25.197,27.298,27.762,29.531 and 29.902 as 1～No. 9 peak.The gas phase color of Oleum Santali albi c
Spectrogram is referring to Fig. 1.According to the gas chromatogram of Oleum Santali albi a～e, obtain the parameter of their each characteristic peaks, be listed in table 1.
The gas chromatogram characteristic peak area of the Lignum Santali Albi oil extract of table 1 separate sources
| Lignum Santali Albi oil extract species | Oleum Santali albi a | Oleum Santali albi b | Oleum Santali albi c | Oleum Santali albi d | Oleum Santali albi e |
| No. 1 peak | 1.75270% | 2.07514% | 4.45137% | 0.20378% | — |
| No. 2 peaks | 3.52195% | 2.10358% | 10.6831% | 0.19826% | — |
| No. 3 peaks | 2.96404% | 3.20674% | 3.84277% | — | 10.77894% |
| No. 4 peaks | 3.83264% | 9.49627% | 2.46444% | 1.43362% | 0.46811% |
| No. 5 peaks | 20.77043% | 1.34512% | 4.17025% | 3.18372% | — |
| No. 6 peaks | 21.90653% | 16.05219% | 27.67051% | — | — |
| No. 7 peaks | 16.66199% | 30.13516% | 25.43301% | — | — |
| No. 8 peaks | 4.68833% | 1.43918% | 1.60963% | 1.61457% | — |
| No. 9 peaks | 8.11181% | 2.23520% | 5.17385% | — | — |
As shown in Table 1, Oleum Santali albi a, b is identical with the position of the characteristic peak of c, illustrates that main component is coincide；But, three it
Between peak area distinct, illustrate that each component content is different.The gas chromatogram characteristic peak of Oleum Santali albi d and e and Oleum Santali albi
A, b and c differ greatly, and the main component without Oleum Santali albi is described.
Experimental example
1. laboratory animal and reagent
1.1 animal packet
Select healthy new zealand rabbit 50, male and female half and half (are bought on behalf, raised by Inst. of Traditional Chinese Medicine, Zhejiang Prov animal experimental center
Support), it is grouped as follows: model group, positive group, negative group, Oleum Santali albi a group, Oleum Santali albi b group, Oleum Santali albi c group, Oleum Santali albi d group, wingceltis
Oleum sesami e group, nitrolingual spray group, are equally divided into 10 groups by each the weight of animals difference, every group 5.Each group administering mode is such as
Under:
(1) model group: Sublingual instills distilled water.
(2) positive group: Sublingual instills nitroglycerin medicinal liquid.
(3) negative group: Sublingual instills the medicinal liquid lacking Oleum Santali albi.
(4) Oleum Santali albi a group: Sublingual instills the medicinal liquid containing Oleum Santali albi a.
(5) Oleum Santali albi b group: Sublingual instills the medicinal liquid containing Oleum Santali albi b.
(6) Oleum Santali albi c group: Sublingual instills the medicinal liquid of c containing Oleum Santali albi.
(7) Oleum Santali albi d group: Sublingual instills the medicinal liquid containing Oleum Santali albi d.
(8) Oleum Santali albi e group: Sublingual instills the medicinal liquid containing Oleum Santali albi e.
(9) nitrolingual spray group: sublingual spraying nitrolingual spray.
1.2 experiment reagent
Nitrolingual spray is Jingwei Pharmaceutical Co., Ltd., Shandong's product, lot number: 1111031.Remaining reagent is city
Sell product.Nitroglycerine tablets are Beijing Yi Min medicinal liquid company limited product, lot number: 20120208.
2. Preparatory work of experiment
2.1 drug solution preparing
Medicinal liquid forms: 140ml containing volatile oil in 625ml medicinal liquid, wherein Oleum Asari: Oleum Santali albi: Rhizoma Alpiniae Officinarum oil: Fructus Piperis Longi oil=
23:70:32:15 (volume ratio), Borneolum Syntheticum 22.5g, remaining as dehydrated alcohol, medicinal liquid density is 0.83g/ml.
Oleum Santali albi a～e group medicinal liquid is prepared respectively according to the above ratio, every group of medicinal liquid 10ml.It is not added with Oleum Santali albi in negative group, with
Dehydrated alcohol replaces.
2.2 dosages calculate
Press " pharmacological experimental methodology " third edition dosage conversions form, the dose ratio checking in people with rabbit (1.5kg) is 14.2.
Tested by clinical dosage 5 times amount (i.e. 19.53 μ l/kg), administration is instilled with microsyringe Sublingual.For nitroglycerin gas
Mist agent, often spray is equivalent to nitroglycerin 0.5mg, carries out by 1 times of discharge rate.For nitroglycerin medicinal liquid, nitroglycerine tablets are clinical to be used
Measure as 0.5mg, new zealand rabbit is pressed 5 times of clinical dosage (i.e. 0.117mg/kg) and used, compound concentration is the nitroglycerin of 1mg/ml
Suspension (now with the current, cool dark place preserves), dosage v=117 μ l/kg.
3. experimental technique
3.1 modelings and administration
Rabbit auricular vein injects 3% pentobarbital sodium (1ml/kg) so as to light anesthesia, Baoding of lying on the back.Record is normal
After electrocardiogram 5min, auricular vein injection of pituitrin 2u/kg (requires at the uniform velocity to inject in 10s), observes v lead electrocardiogram
Situation of change.T ripple is substantially raised, and s-t section is raised above 0.1mv, and between q-t, the phase is obviously prolonged, arrhythmia, then Acute myocardial
Ischemia modeling success.
After each test rabbit anesthesia, record 5min normal ECG.After auricular vein injection of pituitrin (2u/kg), by
Sublingual administration each group medicinal liquid, re-records electrocardiogram in 30min.To before modeling, pituitrin inject latter 15 seconds, administration after 15
Second, latter 1 minute of administration, latter 2 minutes of administration and in good time labelling recording during latter 10 minutes of administration.
3.2 Electrocardiography
Observe electrocardiogram each measuring point t wave height (mv), s-t section height (mv), record modeling before, pituitrin injection
When 15 seconds afterwards, latter 15 seconds of administration, latter 1 minute of administration, latter 2 minutes of administration and latter 10 minutes of administration, t ripple, the height of s-t section, and count
Calculate pituitrin injection after lifting height and administration after down ratio.Data above is by medlab Biological Signal Collecting System
Automatically analyze, the v that the data of all observation index derives from Cor Leporis electrographic recording file leads.
3.3 serum lactate dehydrogenase (SLD)s (ldh), creatine kinase (ck) Activity determination
24h is observed, auricular vein takes blood, after 1 hour, 3000rpm is centrifuged 10min to standing, separates serum after postoperative administration
The level of censorship ldh, ck afterwards.
4. experimental result
4.1 ECG results
Data acquisition, collection are carried out to each group animal electrocardiogram sample point, with spss18.0 to each in addition to model group
Before and after group experiment, the t of labelling point is involved s-t section fall and is compared with model group, by negative group, separate sources Lignum Santali Albi group
Deng experiment before and after labelling point t ripple fall with the positive group be compared, statistical analysis, t involves s-t section fall d
All calculated as the following formula:
In formula, v1Inject latter 15 seconds t for pituitrin and involve s-t section current potential；v2Involve s-t section for day part t after administration
Current potential.Acquired results are shown in Table 2 and 3.
Table 2 administration day part t ripple declines percentage ratio t check table
*: compared with model group, p < 0.05；*: compared with model group, p < 0.01；△: compared with positive group, p < 0.05；
△ △: compared with positive group, p < 0.01.
Table 3 administration day part s-t section declines percentage ratio t check table
*: compared with model group, p < 0.05；*: compared with model group, p < 0.01；△: compared with positive group, p < 0.05；
△ △: compared with positive group, p < 0.01.
From table 2, positive group, Oleum Santali albi a group, Oleum Santali albi b group, Oleum Santali albi c group, nitrolingual spray group is in administration
Afterwards 15 seconds and 1 minute when, t ripple fall is all statistically significant compared with model group.Oleum Santali albi b group, Oleum Santali albi c group, nitre
Acid glycerol aerosol group upon administration 15 seconds and 1 minute when, compare no significant difference with positive group, Oleum Santali albi a group upon administration 1
During minute, compare no significant difference with positive group.
As shown in Table 3, positive group, Oleum Santali albi b group, Oleum Santali albi c group, nitrolingual spray group 15 seconds and 1 upon administration
Minute, Oleum Santali albi a group is administered latter 15 seconds, and s-t section fall is all statistically significant compared with model group.Oleum Santali albi a group, wingceltis
Oleum sesami b group, Oleum Santali albi c group, nitrolingual spray group are not statistically significant compared with positive group.In administration 2 minutes and 10 points
Zhong Shi, positive group compares all no significant differences with model group.This illustrates when 2 minutes, pituitrin event resolves.
It is the Electrocardiogram Feature of Acute myocardial ischemia or heart infarction that t ripple and s-t section are raised, during by being administered latter 15 seconds and 1 minute
Statistic analysis result understand, Oleum Santali albi b group, Oleum Santali albi c group and nitrolingual spray group can decline t ripple at short notice
And s-t section highly and remains stable, and more positive group of Oleum Santali albi a group onset is slow, and can slowly decline t involves s-t section height,
Fall and positive group no significant difference when 1 minute.This explanation sweet aerosol of nitre and Oleum Santali albi b group, Oleum Santali albi c group are joined
The medicinal liquid obtaining has effective effect faster to alleviating acute angina pectoris outbreak.The medicinal liquid that Oleum Santali albi a group product are joined was at 1 minute
Inside can reach the effect of allevating angina pectoris.
4.2 biochemical indicator results
In ear edge vein exploitating blood before each group zoopery, separate serum；And after the test 24 hours again auricular vein adopt
Blood, separation serum.After each group blood sampling, ldh, ck changing value is shown in Table 4.
Table 4 serum ldh, ck detect table
Find, animal serum has larger individual variation by the physicochemical data analysis before being administered, therefore do not carry out statistics
Analysis.From table 4, compared with model group, ldh and ck has all declined each administration group, but fall is not high.This explanation is each
Administration group, after medicine effect, produces certain impact to Biochemical Indices In Serum, inhibits the rising of ldh and ck to a certain extent.
To sum up, from electrocardiogram t ripple fall and s-t section fall and comprehensive Analysis of Biochemical, Oleum Santali albi
B group, Oleum Santali albi c group drug effect the most obvious.
In the case of the flesh and blood without departing substantially from the present invention, it may occur to persons skilled in the art that any deformation, change
Enter, replace and each fall within the scope of the present invention.
Claims (10)
1. a kind of Lignum Santali Albi oil extract is it is characterised in that the gas chromatogram of this Lignum Santali Albi oil extract includes following characteristic peak:
Wherein, the unit of described retention time is minute, and its deviation is ± 5%；Described peak area is with this Lignum Santali Albi oil extract
Calculated on the basis of the overall peak area of gas chromatogram.
2. Lignum Santali Albi oil extract according to claim 1 is it is characterised in that described retention time and peak area are as follows:
3. Lignum Santali Albi oil extract according to claim 1 is it is characterised in that the gas chromatogram of this Lignum Santali Albi oil extract is also wrapped
Include following characteristic peak:
Wherein, the unit of described retention time is minute, and its deviation is ± 5%；Described peak area is with this Lignum Santali Albi oil extract
Calculated on the basis of the overall peak area of gas chromatogram.
4. Lignum Santali Albi oil extract according to claim 1 is it is characterised in that the gas chromatogram of this Lignum Santali Albi oil extract is also wrapped
Include following characteristic peak:
Wherein, the unit of described retention time is minute, and its deviation is ± 5%；Described peak area is with this Lignum Santali Albi oil extract
Calculated on the basis of the overall peak area of gas chromatogram.
5. Lignum Santali Albi oil extract according to claim 1 is it is characterised in that the spy of the gas chromatogram of this Lignum Santali Albi oil extract
Levy peak selected from one of following two stack features peaks:
(1) first stack features peak:
(2) second stack features peaks:
Wherein, the unit of described retention time is minute, and its deviation is ± 5%；Described peak area is with this Lignum Santali Albi oil extract
Calculated on the basis of the overall peak area of gas chromatogram, its deviation is ± 3%.
6. the Lignum Santali Albi oil extract according to any one of Claims 1 to 5 is it is characterised in that described gas chromatogram is passed through such as
Lower method obtains:
(1) Oleum Santali albi is adopted 8～10 times of diluted ethyl acetate；
(2) using the quartz capillary column that length is 30m, internal diameter is 0.25mm and fixing phase coating thickness of liquid film is 0.25 μm,
Sampling condition is as follows:
Injector temperature is 250 DEG C, 250 DEG C of fid detector temperature, and carrier gas is helium, and flow velocity is 1.0ml/min, and split ratio is
40:1, sample size is 0.2 μ l；
Heating schedule is as follows:
Column temperature is 90 DEG C, is warming up to 160 DEG C with the heating rate of 5 DEG C/min, then is heated up 166 DEG C with the heating rate of 1 DEG C/min,
Then it is incubated 5min, then is heated up 167 DEG C with the heating rate of 0.5 DEG C/min, be incubated 5min, then the intensification speed with 0.5 DEG C/min
Rate is warming up to 168 DEG C, is incubated 2min, is finally warming up to 230 DEG C with the heating rate of 10 DEG C/min.
7. the detection method of the Lignum Santali Albi oil extract according to any one of Claims 1 to 5 is it is characterised in that described side
Method comprises the steps:
(1) Oleum Santali albi is adopted 8～10 times of diluted ethyl acetate；
(2) using the quartz capillary column that length is 30m, internal diameter is 0.25mm and fixing phase coating thickness of liquid film is 0.25 μm,
Sampling condition is as follows:
Injector temperature is 250 DEG C, 250 DEG C of fid detector temperature, and carrier gas is helium, and flow velocity is 1.0ml/min, and split ratio is
40:1, sample size is 0.2 μ l；
Heating schedule is as follows:
Column temperature is 90 DEG C, is warming up to 160 DEG C with the heating rate of 5 DEG C/min, then is heated up 166 DEG C with the heating rate of 1 DEG C/min,
Then it is incubated 5min, then is heated up 167 DEG C with the heating rate of 0.5 DEG C/min, be incubated 5min, then the intensification speed with 0.5 DEG C/min
Rate heats up 168 DEG C, is incubated 2min, is finally warming up to 230 DEG C with the heating rate of 10 DEG C/min.
8. a kind of for treatment or allevating angina pectoris pharmaceutical composition it is characterised in that this pharmaceutical composition comprise right will
Seek the Lignum Santali Albi oil extract described in 1～6 any one.
9. pharmaceutical composition according to claim 8 is it is characterised in that this pharmaceutical composition also includes Oleum Asari, Gao Liang
Oil of ginger, Fructus Piperis Longi oil, Borneolum Syntheticum and pharmaceutically acceptable adjuvant.
10. the Lignum Santali Albi oil extract according to any one of claim 1～6 is in the medicine system of preparation treatment or allevating angina pectoris
Purposes in agent.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111073754A (en) * | 2019-09-12 | 2020-04-28 | 天津芸熙生物技术有限公司 | Method for extracting sandalwood seed oil from macadamia ternifolia fruits |
| CN112697939A (en) * | 2020-12-23 | 2021-04-23 | 广西壮族自治区食品药品检验所 | Method for determining multiple components in wide chest aerosol based on GC-MS/MS |
| CN115420832A (en) * | 2022-09-20 | 2022-12-02 | 贵州医科大学 | GC-MS fingerprint spectrum and multi-index content determination method of litsea pungens oil |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1732908A (en) * | 2004-08-09 | 2006-02-15 | 晏四平 | Borneol contained spray |
| CN105733814A (en) * | 2016-04-07 | 2016-07-06 | 广西贵港市宏利生物能源科技有限公司 | Distilling production method for sandalwood essential oil and equipment |
-
2016
- 2016-08-30 CN CN201610778995.3A patent/CN106361785A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1732908A (en) * | 2004-08-09 | 2006-02-15 | 晏四平 | Borneol contained spray |
| CN105733814A (en) * | 2016-04-07 | 2016-07-06 | 广西贵港市宏利生物能源科技有限公司 | Distilling production method for sandalwood essential oil and equipment |
Non-Patent Citations (1)
| Title |
|---|
| 国家药典委员会: "《关于2015年版《中国药典》（一部）品种的公示》", 4 August 2014 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111073754A (en) * | 2019-09-12 | 2020-04-28 | 天津芸熙生物技术有限公司 | Method for extracting sandalwood seed oil from macadamia ternifolia fruits |
| CN111073754B (en) * | 2019-09-12 | 2022-12-27 | 天津芸熙生物技术有限公司 | Method for extracting sandalwood seed oil from macadamia ternifolia fruits |
| CN112697939A (en) * | 2020-12-23 | 2021-04-23 | 广西壮族自治区食品药品检验所 | Method for determining multiple components in wide chest aerosol based on GC-MS/MS |
| CN112697939B (en) * | 2020-12-23 | 2022-04-26 | 广西壮族自治区食品药品检验所 | Method for determining multiple components in wide chest aerosol based on GC-MS/MS |
| CN115420832A (en) * | 2022-09-20 | 2022-12-02 | 贵州医科大学 | GC-MS fingerprint spectrum and multi-index content determination method of litsea pungens oil |
| CN115420832B (en) * | 2022-09-20 | 2024-03-19 | 贵州医科大学 | GC-MS fingerprint spectrum and multi-index content determination method for litsea coreana oil |
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