CN106360717A - Production method of W/O blueberry anthocyanin micro-emulsion - Google Patents
Production method of W/O blueberry anthocyanin micro-emulsion Download PDFInfo
- Publication number
- CN106360717A CN106360717A CN201610724745.1A CN201610724745A CN106360717A CN 106360717 A CN106360717 A CN 106360717A CN 201610724745 A CN201610724745 A CN 201610724745A CN 106360717 A CN106360717 A CN 106360717A
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- China
- Prior art keywords
- water
- microemulsion
- oil type
- anthocyanidin
- manufacture method
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000004530 micro-emulsion Substances 0.000 title claims abstract description 74
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 33
- 240000000851 Vaccinium corymbosum Species 0.000 title claims abstract description 7
- 235000003095 Vaccinium corymbosum Nutrition 0.000 title claims abstract description 7
- 235000017537 Vaccinium myrtillus Nutrition 0.000 title claims abstract description 7
- 235000021014 blueberries Nutrition 0.000 title claims abstract description 7
- 235000010208 anthocyanin Nutrition 0.000 title abstract description 7
- 229930002877 anthocyanin Natural products 0.000 title abstract description 7
- 239000004410 anthocyanin Substances 0.000 title abstract description 7
- 150000004636 anthocyanins Chemical class 0.000 title abstract description 7
- 238000000034 method Methods 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000004094 surface-active agent Substances 0.000 claims abstract description 17
- HIQIXEFWDLTDED-UHFFFAOYSA-N 4-hydroxy-1-piperidin-4-ylpyrrolidin-2-one Chemical compound O=C1CC(O)CN1C1CCNCC1 HIQIXEFWDLTDED-UHFFFAOYSA-N 0.000 claims abstract description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000006228 supernatant Substances 0.000 claims abstract description 13
- 239000008367 deionised water Substances 0.000 claims abstract description 10
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 10
- 235000013305 food Nutrition 0.000 claims abstract description 8
- 150000002632 lipids Chemical class 0.000 claims abstract description 7
- 229930014669 anthocyanidin Natural products 0.000 claims description 52
- 235000008758 anthocyanidins Nutrition 0.000 claims description 52
- 241000727741 Vaccinium oxycoccos Species 0.000 claims description 27
- 235000002118 Vaccinium oxycoccus Nutrition 0.000 claims description 27
- 239000000049 pigment Substances 0.000 claims description 27
- 238000013329 compounding Methods 0.000 claims description 12
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 12
- 239000004064 cosurfactant Substances 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 4
- 239000002775 capsule Substances 0.000 claims description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 2
- 229920000053 polysorbate 80 Polymers 0.000 claims description 2
- 150000001452 anthocyanidin derivatives Chemical class 0.000 claims 11
- NWGKJDSIEKMTRX-MDZDMXLPSA-N Sorbitan oleate Chemical compound CCCCCCCC\C=C\CCCCCCCC(=O)OCC(O)C1OCC(O)C1O NWGKJDSIEKMTRX-MDZDMXLPSA-N 0.000 claims 1
- 239000002245 particle Substances 0.000 abstract description 7
- 230000015556 catabolic process Effects 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000006731 degradation reaction Methods 0.000 abstract 1
- 239000002994 raw material Substances 0.000 abstract 1
- 150000001453 anthocyanidins Chemical class 0.000 description 41
- 239000003921 oil Substances 0.000 description 41
- 238000002360 preparation method Methods 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 5
- 210000000481 breast Anatomy 0.000 description 3
- 239000012456 homogeneous solution Substances 0.000 description 3
- 239000003094 microcapsule Substances 0.000 description 3
- 230000003213 activating effect Effects 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- -1 flavone compound Chemical class 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 235000009392 Vitis Nutrition 0.000 description 1
- 241000219095 Vitis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 235000002864 food coloring agent Nutrition 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000003475 lamination Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000593 microemulsion method Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a production method of a W/O blueberry anthocyanin micro-emulsion. The method includes the steps of: 1) adding the following raw materials at 25 DEG C according to ratio: 8-12 g of a compound surfactant being 7.5 in HLB value, 4-6 g of a co-surfactant anhydrous ethanol, 1.15-4.59 g of deionized water, and 12-18 g of isopropyl myristate, and uniformly mixing the components in a vortex mixer until a homogeneous and transparent solution is formed; and 2) maintaining the temperature at 25 DEG C, adding prepared anthocyanin to a W/O blank micro-emulsion in different ratios, performing centrifugation to obtain a supernatant, which is the W/O blueberry anthocyanin micro-emulsion. The method is simple in operations. The micro-emulsion is 10-100 nm in particle size and is easy to absorb due to the small particle size, and has good stability, wherein active components are in the inner layer of the micro-emulsion, so that influence on degradation of the anthocyanin due to light irradiation and pH value. The micro-emulsion has good fat solubility, which is improved by 30%, so that the applications of anthocyanin in lipid foods are increased.
Description
Technical field
The invention belongs to biologic product technology field, more particularly, to a kind of making side of water-in-oil type vaccinium oxycoccus pigment microemulsion
Method.
Background technology
Anthocyanidin is flavone compound, also known as anthocyanin.It is widely present in the plants such as Fructus Vitis viniferae, Rhizoma Steudnerae Henryanae, Fructus Mori, blue berry
In.Anthocyanidin is more sensitive to environment, can assume different colours under different ph, is a kind of natural food color;Additionally,
Research shows, anthocyanidin has multiple physiological active functionses such as antioxidation, defying age, prevention cardiovascular and cerebrovascular disease.Based on cyanine
The physiological function of element, it has huge potential using value in health product and medicine.But, due to anthocyanidin stability relatively
Difference, in oil substances, dissolubility is relatively low, which greatly limits anthocyanidin in food, the especially application in lipid food.As
What improves the stability of anthocyanidin, and the fat-soluble and bioavailability increasing anthocyanidin becomes research Jiao of domestic and international expert
One of point.
At present, study both at home and abroad and mainly pass through embedding techniqueses for improving the stability of anthocyanidin.Anthocyanidin microcapsule is
Using certain wall material, anthocyanidin embedding is got up, and then increase the stability of anthocyanidin.Anthocyanidin microcapsule has been widely used
In industrialized production, but because anthocyanidin microcapsule granule is larger, the absorption rate of human body is relatively low.Water oil-packaging type micro-emulsion is
The microemulsion that surfactant, cosurfactant, oil phase and aqueous phase are formed according to certain ratio, increases to a certain extent
Add the stability of anthocyanidin, particle diameter is less, be easy to absorb.But, dissolubility in lipid food for the oil-in-water type anthocyanidin
Relatively low, which greatly limits use in lipid food for the anthocyanidin.
Content of the invention
It is an object of the invention to provide a kind of manufacture method of water-in-oil type vaccinium oxycoccus pigment microemulsion is it is intended to solve existing
Anthocyanidin biological preparation there is a problem of that dissolubility is low and stability is low.
The present invention is achieved in that a kind of manufacture method of water-in-oil type vaccinium oxycoccus pigment microemulsion, described water-in-oil type
The manufacture method of vaccinium oxycoccus pigment microemulsion comprises the following steps:
Step one, by the surfactant for 7.5 for the compounding hlb value, (this surfactant is pressed by Tween 80 and sorbester p17
Compound according to mass ratio 3.2:7.5 and form) 8g~12g and cosurfactant dehydrated alcohol 4g~6g, deionized water 1.15g~
4.59g, isopropyl myristate 12g~18g proportionally add at 25 DEG C, and mediation blender mixes, until being formed homogeneous
Bright solution;
Step 2, keeping temperature is 25 DEG C, the anthocyanidin 1g making, and is added in water-in-oil type blank microemulsion, centrifuging and taking
Supernatant, as water-in-oil type anthocyanidin microemulsion.
Further, the manufacture method of described water-in-oil type vaccinium oxycoccus pigment microemulsion comprises the following steps:
Step one, by the compounding hlb value surfactant 8g for 7.5 and cosurfactant dehydrated alcohol 4g, go from
Sub- water 1.15g, isopropyl myristate 18g proportionally add at 25 DEG C, and mediation blender mixes, until forming transparent and homogeneous
Solution;
Step 2, keeping temperature is 25 DEG C, the anthocyanidin making is added to water-in-oil type blank according to different proportion micro-
In breast, centrifuging and taking supernatant, as water-in-oil type anthocyanidin microemulsion.
Further, the manufacture method of described water-in-oil type vaccinium oxycoccus pigment microemulsion comprises the following steps:
Step one, by the compounding hlb value surfactant 10g for 7.5 and cosurfactant dehydrated alcohol 5g, go from
Sub- water 2.73g, isopropyl myristate 15g proportionally add at 25 DEG C, and mediation blender mixes, until forming transparent and homogeneous
Solution;
Step 2, keeping temperature is 25 DEG C, the anthocyanidin making is added to water-in-oil type blank according to different proportion micro-
In breast, centrifuging and taking supernatant, as water-in-oil type anthocyanidin microemulsion.
Further, the manufacture method of described water-in-oil type vaccinium oxycoccus pigment microemulsion comprises the following steps:
Step one, by the compounding hlb value surfactant 12g for 7.5 and cosurfactant dehydrated alcohol 6g, go from
Sub- water 4.59g, isopropyl myristate 12g proportionally add at 25 DEG C, and mediation blender mixes, until forming transparent and homogeneous
Solution;
Step 2, keeping temperature is 25 DEG C, the anthocyanidin making is added to water-in-oil type blank according to different proportion micro-
In breast, centrifuging and taking supernatant, as water-in-oil type anthocyanidin microemulsion.
Another object of the present invention is to providing a kind of manufacture method system by described water-in-oil type vaccinium oxycoccus pigment microemulsion
The lipid method for making food of the water-in-oil type vaccinium oxycoccus pigment microemulsion made.
Another object of the present invention is to providing a kind of manufacture method system by described water-in-oil type vaccinium oxycoccus pigment microemulsion
The cake manufacture method of the water-in-oil type vaccinium oxycoccus pigment microemulsion made.
Another object of the present invention is to providing a kind of manufacture method system by described water-in-oil type vaccinium oxycoccus pigment microemulsion
The capsule made.
The manufacture method of the water-in-oil type vaccinium oxycoccus pigment microemulsion that the present invention provides, blank microemulsion transparent and homogeneous, measure
Particle diameter is all between 10-100nm;Add microemulsion still clear after anthocyanidin;Preparation method is simple, and microemulsion is by surface
Activating agent, cosurfactant, oil phase and aqueous phase spontaneously form according to certain ratio mix homogeneously with transparent molten
Liquid.Its preparation need not be done work in the external world, simple to operate;Particle diameter is little and homogeneous, the particle diameter of microemulsion typically between 10-100nm, particle diameter
Less, it is easy to absorb;Good stability, microemulsion is centrifuged 30min no lamination in 4000r/min;Water-in-oil type anthocyanidin its
Active component, in the internal layer of microemulsion, greatly reduces illumination, the impact that ph degrades to anthocyanidin;Fat-soluble preferable, water-in-oil type
The fat-soluble of microemulsion increases by 30%, which increases use in lipid food for the anthocyanidin.
Brief description
Fig. 1 is the manufacture method flow chart of water-in-oil type vaccinium oxycoccus pigment microemulsion provided in an embodiment of the present invention.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not used to
Limit the present invention.
Below in conjunction with the accompanying drawings the application principle of the present invention is explained in detail.
As shown in figure 1, the manufacture method of the water-in-oil type vaccinium oxycoccus pigment microemulsion of the embodiment of the present invention comprises the following steps:
The preparation of s101: water in oil micro emulsion.By surfactant 8g~12g for 7.5 for the compounding hlb value with help surface
Activating agent dehydrated alcohol 4g~6g, deionized water 1.15g~4.59g, isopropyl myristate 12g~18g 25 DEG C according to than
Example is added, and mediation blender mixes, until forming the solution of transparent and homogeneous;
S102: add the production of the microemulsion of anthocyanidin.Keeping temperature is 25 DEG C, by the anthocyanidin making according to not on year-on-year basis
Example is added in water-in-oil type blank microemulsion.Centrifuging and taking supernatant, as water-in-oil type anthocyanidin microemulsion.
With reference to specific embodiment, the application principle of the present invention is further described.
Embodiment 1:
Step one, the preparation of water in oil micro emulsion.The surfactant 8g for 7.5 for the compounding hlb value is lived with helping surface
Property agent dehydrated alcohol 4g, deionized water 1.15g, isopropyl myristate 18g proportionally add at 25 DEG C, mediation blender mixes
Even, until forming the solution of transparent and homogeneous;
Step 2, adds the production of the microemulsion of anthocyanidin.Keeping temperature is 25 DEG C, by the anthocyanidin making according to difference
Ratio is added in water-in-oil type blank microemulsion.Centrifuging and taking supernatant, as water-in-oil type anthocyanidin microemulsion.
Embodiment 2:
Step one, the preparation of water in oil micro emulsion.The surfactant 10g for 7.5 for the compounding hlb value is lived with helping surface
Property agent dehydrated alcohol 5g, deionized water 2.73g, isopropyl myristate 15g proportionally add at 25 DEG C, mediation blender mixes
Even, until forming the solution of transparent and homogeneous;
Step 2, adds the production of the microemulsion of anthocyanidin.Keeping temperature is 25 DEG C, by the anthocyanidin making according to difference
Ratio is added in water-in-oil type blank microemulsion.Centrifuging and taking supernatant, as water-in-oil type anthocyanidin microemulsion.
Embodiment 3:
Step one, the preparation of water in oil micro emulsion.The surfactant 12g for 7.5 for the compounding hlb value is lived with helping surface
Property agent dehydrated alcohol 6g, deionized water 4.59g, isopropyl myristate 12g proportionally add at 25 DEG C, mediation blender mixes
Even, until forming the solution of transparent and homogeneous;
Step 2, adds the production of the microemulsion of anthocyanidin.Keeping temperature is 25 DEG C, by the anthocyanidin making according to difference
Ratio is added in water-in-oil type blank microemulsion.Centrifuging and taking supernatant, as water-in-oil type anthocyanidin microemulsion.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Any modification, equivalent and improvement made within god and principle etc., should be included within the scope of the present invention.
Claims (7)
1. a kind of manufacture method of water-in-oil type vaccinium oxycoccus pigment microemulsion is it is characterised in that described water-in-oil type vaccinium oxycoccus pigment
The manufacture method of microemulsion comprises the following steps:
Step one, by the compounding hlb value surfactant 8g~12g for 7.5 and cosurfactant dehydrated alcohol 4g~6g,
Deionized water 1.15g~4.59g, isopropyl myristate 12g~18g proportionally add at 25 DEG C, and mediation blender mixes,
Until forming the solution of transparent and homogeneous;Surfactant is compounded according to mass ratio 3.2:7.5 by Tween 80 and sorbester p17 and forms;
Step 2, keeping temperature is 25 DEG C, and the anthocyanidin making is added in water-in-oil type blank microemulsion according to different proportion,
Centrifuging and taking supernatant, as water-in-oil type anthocyanidin microemulsion.
2. the manufacture method of water-in-oil type vaccinium oxycoccus pigment microemulsion as claimed in claim 1 is it is characterised in that described Water-In-Oil
The manufacture method of type vaccinium oxycoccus pigment microemulsion comprises the following steps:
Step one, by the surfactant 8g for 7.5 for the compounding hlb value and cosurfactant dehydrated alcohol 4g, deionized water
1.15g, isopropyl myristate 18g proportionally add at 25 DEG C, and mediation blender mixes, until forming the molten of transparent and homogeneous
Liquid;
Step 2, keeping temperature is 25 DEG C, and the anthocyanidin making is added in water-in-oil type blank microemulsion according to different proportion,
Centrifuging and taking supernatant, as water-in-oil type anthocyanidin microemulsion.
3. the manufacture method of water-in-oil type vaccinium oxycoccus pigment microemulsion as claimed in claim 1 is it is characterised in that described Water-In-Oil
The manufacture method of type vaccinium oxycoccus pigment microemulsion comprises the following steps:
Step one, by the surfactant 10g for 7.5 for the compounding hlb value and cosurfactant dehydrated alcohol 5g, deionized water
2.73g, isopropyl myristate 15g proportionally add at 25 DEG C, and mediation blender mixes, until forming the molten of transparent and homogeneous
Liquid;
Step 2, keeping temperature is 25 DEG C, and the anthocyanidin making is added in water-in-oil type blank microemulsion according to different proportion,
Centrifuging and taking supernatant, as water-in-oil type anthocyanidin microemulsion.
4. the manufacture method of water-in-oil type vaccinium oxycoccus pigment microemulsion as claimed in claim 1 is it is characterised in that described Water-In-Oil
The manufacture method of type vaccinium oxycoccus pigment microemulsion comprises the following steps:
Step one, by the surfactant 12g for 7.5 for the compounding hlb value and cosurfactant dehydrated alcohol 6g, deionized water
4.59g, isopropyl myristate 12g proportionally add at 25 DEG C, and mediation blender mixes, until forming the molten of transparent and homogeneous
Liquid;
Step 2, keeping temperature is 25 DEG C, and the anthocyanidin making is added in water-in-oil type blank microemulsion according to different proportion,
Centrifuging and taking supernatant, as water-in-oil type anthocyanidin microemulsion.
5. the water-in-oil type blue berry that a kind of manufacture method of water-in-oil type vaccinium oxycoccus pigment microemulsion described in claim 1-4 makes
The lipid method for making food of anthocyanidin microemulsion.
6. the water-in-oil type blue berry that a kind of manufacture method of water-in-oil type vaccinium oxycoccus pigment microemulsion described in claim 1-4 makes
The cake manufacture method of anthocyanidin microemulsion.
7. the water-in-oil type blue berry that a kind of manufacture method of water-in-oil type vaccinium oxycoccus pigment microemulsion described in claim 1-4 makes
The capsule of anthocyanidin microemulsion.
Priority Applications (1)
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CN201610724745.1A CN106360717A (en) | 2016-08-25 | 2016-08-25 | Production method of W/O blueberry anthocyanin micro-emulsion |
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CN201610724745.1A CN106360717A (en) | 2016-08-25 | 2016-08-25 | Production method of W/O blueberry anthocyanin micro-emulsion |
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Family
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CN201610724745.1A Pending CN106360717A (en) | 2016-08-25 | 2016-08-25 | Production method of W/O blueberry anthocyanin micro-emulsion |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111743863A (en) * | 2020-08-03 | 2020-10-09 | 海南大学 | Preparation method of water-in-oil type blueberry anthocyanin microemulsion |
CN114651979A (en) * | 2022-03-29 | 2022-06-24 | 南昌大学 | Transparent anthocyanin self-associating emulsion based on internal water phase microenvironment regulation and control and preparation method thereof |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103599067A (en) * | 2013-10-29 | 2014-02-26 | 盐城工学院 | W/O microemulsion, and preparation method and applications thereof |
CN105394521A (en) * | 2015-11-04 | 2016-03-16 | 天津科技大学 | Water-in-oil type purple sweet potato pigment micro-emulsion and preparation method thereof |
-
2016
- 2016-08-25 CN CN201610724745.1A patent/CN106360717A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103599067A (en) * | 2013-10-29 | 2014-02-26 | 盐城工学院 | W/O microemulsion, and preparation method and applications thereof |
CN105394521A (en) * | 2015-11-04 | 2016-03-16 | 天津科技大学 | Water-in-oil type purple sweet potato pigment micro-emulsion and preparation method thereof |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111743863A (en) * | 2020-08-03 | 2020-10-09 | 海南大学 | Preparation method of water-in-oil type blueberry anthocyanin microemulsion |
CN114651979A (en) * | 2022-03-29 | 2022-06-24 | 南昌大学 | Transparent anthocyanin self-associating emulsion based on internal water phase microenvironment regulation and control and preparation method thereof |
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