CN106359930A - Feed for producing high EPA and DPA content functional beef - Google Patents
Feed for producing high EPA and DPA content functional beef Download PDFInfo
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- CN106359930A CN106359930A CN201610745356.7A CN201610745356A CN106359930A CN 106359930 A CN106359930 A CN 106359930A CN 201610745356 A CN201610745356 A CN 201610745356A CN 106359930 A CN106359930 A CN 106359930A
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Abstract
The present invention relates to the technical field of feeds for domestic poultries and particularly discloses a feed for producing high EPA and DPA content functional beef. The beef feed consists of a common feed for cattle and fermented nannochloropsis for producing high DHA content functional beef. The fermented nannochloropsis is obtained by a fermentation of using ground nannochloropsis to inoculate bacillus cereus seed liquid. According to the principles and technologies of cattle physiology and nutrition, the fermented nannochloropsis is used as an additive to improve the fatty acid composition of the cattle daily diets and improve the enrichment degree of the EPA and DPA in the beef, thus the high DHA content functional beef is produced.
Description
Technical field
The present invention relates to poultry feed technical field is and in particular to a kind of feature high for producing epa, dpa content
Beef feedstuff.
Background technology
Epa belongs to ω -3 series polyunsaturated fatty acid, is that human body itself can not synthesize but indispensable important battalion
Support element, therefore referred to as essential fatty acid.Although linolenic acid can be converted into epa in human body, this reaction is in human body
Speed is very slow and inversion quantity seldom, human body far can not be met to the needs of epa it is therefore necessary to directly supplement from food.
Dpa is the long-chain unsaturated fatty acid just having in mankind's colostrum, and it is equally the master of human brain tissue, neurocyte
Want composition, dpa content in human milk and seal oil is high, be to lack in fish oil and various food.
Beef is one of important meat sources in people's meals, has protein content height, and fat content is low, taste
Deliciousness a little, is liked by broad masses of the people deeply, but, in the conventional beef producing, epa, dpa content is relatively low.
Content of the invention
It is an object of the invention to provide a kind of feature beef feedstuff high for producing epa, dpa content.
The technical solution used in the present invention is, a kind of feature beef feedstuff high for producing epa, dpa content,
Described beef feedstuff intends, by cattle normal diet and fermentation are micro-, the work(high for producing epa, dpa content that ball algae forms
Can property beef feedstuff；
Cattle normal diet and fermentation micro- plan ball algae are higher for producing epa, dpa content than composition by the weight of 100:2-2.5
Feature beef feedstuff；
The feature beef high for producing epa, dpa content is obtained after cattle normal diet and the direct mixing of fermentation micro- plan ball algae
Feedstuff；
Described fermentation is micro- to intend ball algae when making,
(1) algae cell density is taken to be 5x107The micro- ball algae solution of intending of cells/ml prepares micro- plan ball algae solution fermentation medium, micro- to every l
Intend adding nan03 0.5g, mgso4 7h2o 4g, fes04 6g, sucrose 15g in ball algae solution, natural ph value, at 150 DEG C
Sterilizing 10min, is cooled to room temperature；
(2) with 2% inoculation ratio addition Deshi Lactobacilluss bacterium solution (inoculum concentration is as 2%v/v) in the solution of step (2), mix homogeneously,
Then in 30 DEG C, under the conditions of 220rpm, cultivate 24h.
Deshi Lactobacilluss bacterium solution Zhong Deshi living preparation of lactobacillus number is 1 × 108More than cfu/ml.
The present invention, according to the philosophy and technique of cattle physiology, threpsology, by the use of micro- plan ball algae of fermenting as additive, improves
The fatty acid composition of cattle daily ration, improves epa, dpa enrichment degree in beef, thus producing the high function of dha content
Type beef.
Specific embodiment
The present invention is described in further detail following by using embodiment and comparative examples.However, the present invention
Do not limited by following examples；
Embodiment 1, with the present invention be used for produce the high feature of the high feature beef Feed Manufacturing dha content of dha content
Beef.
Experimental animal
Test ox is selected from through the disease-free Luxi Yellow cattle 30 of quarantining, and test ox focused near 12 monthly ages, test be divided into experimental group,
2 three groups of matched group 1, matched group, every group 10,3 groups except feeding feed stuff difference in addition to, other conditions (feeding environment, feeding method)
All same.
The daily feedstuff of experimental group by wheat bran 10%, oilseed meals 15%, conch meal 2%, bone meal 3%, Sal 1%, balance of Semen Maydiss,
The weight that experimental group presses 100:2 in daily feedstuff feeds intake as Weight of Yellow Cattle daily than adding fermentation micro- plan ball algae, daily feedstuff
2%, in addition, cattle can free choice feeding roughage, roughage mainly have corn straw, melon seedling, peanut vine, fresh grass,
Radix Glycyrrhizae.
The daily feedstuff of matched group 1 is by wheat bran 10%, oilseed meals 15%, conch meal 2%, bone meal 3%, Sal 1%, balance of jade
Rice, the weight that matched group 1 presses 100:2 in daily feedstuff feeds intake as Weight of Yellow Cattle daily than adding micro- plan ball algae, daily feedstuff
2%, in addition, cattle can free choice feeding roughage, roughage mainly have corn straw, melon seedling, peanut vine, fresh grass,
Radix Glycyrrhizae.
The daily feedstuff of matched group 2 is by wheat bran 10%, oilseed meals 15%, conch meal 2%, bone meal 3%, Sal 1%, balance of
Semen Maydiss, in addition, cattle can free choice feeding roughage, roughage mainly have corn straw, melon seedling, peanut vine, fresh grass,
Radix Glycyrrhizae.
Wherein, in whole experiment process, the conventional immune programme for children of Luxi Yellow cattle execution, the stress that no disease, environment cause.
Feed to the 30th day, butcher, shoulder elbow meat, chest and abdomen meat, lean pork taken under the spinal column of a hog, the sampling of lower limb buttockss meat, in -20 DEG C of stored refrigerated, analysis is each
The composition of fatty acid in tissue.
Whole experimental period, have no test ox have dysentery, loss of appetite, etc. irregularities.
Wherein, in experimental group beef, in shoulder elbow meat, epa, dpa content is averagely 0.82%, 0.75%；In chest and abdomen meat, epa, dpa contain
Amount is averagely 0.94%, 0.99%；In lean pork taken under the spinal column of a hog, epa, dpa content is averagely 0.53%, 0.55%；Epa, dpa content in lower limb buttockss meat
Averagely 1.88%, 1.64%.
Wherein, in matched group 1 beef, in shoulder elbow meat, epa, dpa content is averagely 0.31%, 0.33%；In chest and abdomen meat, epa, dpa contain
Amount is averagely 0.25%, 0.36%；In lean pork taken under the spinal column of a hog, epa, dpa content is averagely 0.12%, 0.25%；Epa, dpa content in lower limb buttockss meat
Averagely 0.43%, 0.59%.
Wherein, in matched group 2 beef, in shoulder elbow meat, epa, dpa content is averagely 0.20%, 0%；Epa, dpa content in chest and abdomen meat
Averagely 0.22%, 0.41%, in lean pork taken under the spinal column of a hog, epa, dpa content is averagely 0%, 0%；In lower limb buttockss meat, epa, dpa content averagely exists
0.35%、0.40%.
Embodiment 2, with the present invention be used for produce the high work(of the high feature beef Feed Manufacturing dha content of dha content
Can property beef.
Experimental animal
Test ox is selected from through the disease-free Luxi Yellow cattle 30 of quarantining, and test ox focused near 12 monthly ages, test be divided into experimental group,
2 three groups of matched group 1, matched group, every group 10,3 groups except feeding feed stuff difference in addition to, other conditions (feeding environment, feeding method)
All same.
The daily feedstuff of experimental group by wheat bran 10%, oilseed meals 15%, conch meal 2%, bone meal 3%, Sal 1%, balance of Semen Maydiss,
The weight that experimental group presses 100:2.5 in daily feedstuff feeds intake as cattle body daily than adding fermentation micro- plan ball algae, daily feedstuff
The 2% of weight, in addition, cattle can free choice feeding roughage, roughage mainly have corn straw, melon seedling, peanut vine, fresh
Grass, Radix Glycyrrhizae.
The daily feedstuff of matched group 1 is by wheat bran 10%, oilseed meals 15%, conch meal 2%, bone meal 3%, Sal 1%, balance of jade
Rice, the weight that matched group 1 presses 100:2.5 in daily feedstuff feeds intake as cattle body daily than adding micro- plan ball algae, daily feedstuff
The 2% of weight, in addition, cattle can free choice feeding roughage, roughage mainly have corn straw, melon seedling, peanut vine, fresh
Grass, Radix Glycyrrhizae.
The daily feedstuff of matched group 2 is by wheat bran 10%, oilseed meals 15%, conch meal 2%, bone meal 3%, Sal 1%, balance of jade
Rice, in addition, cattle can free choice feeding roughage, roughage mainly have corn straw, melon seedling, peanut vine, fresh grass, dry
Grass.
Wherein, in whole experiment process, the conventional immune programme for children of Luxi Yellow cattle execution, the stress that no disease, environment cause.
Feed to the 30th day, butcher, shoulder elbow meat, chest and abdomen meat, lean pork taken under the spinal column of a hog, the sampling of lower limb buttockss meat, in -20 DEG C of stored refrigerated, analysis is each
The composition of fatty acid in tissue.
Whole experimental period, have no test ox have dysentery, loss of appetite, etc. irregularities.
Wherein, in experimental group beef, in shoulder elbow meat, epa, dpa content is averagely 0.86%, 0.79%；In chest and abdomen meat, epa, dpa contain
Amount is averagely 0.95%, 1.02%；In lean pork taken under the spinal column of a hog, epa, dpa content is averagely 0.61%, 0.59%；Epa, dpa content in lower limb buttockss meat
Averagely 1.93%, 1.70%.
Wherein, in matched group 1 beef, in shoulder elbow meat, epa, dpa content is averagely 0.32%, 0.335%；Epa, dpa in chest and abdomen meat
Content is averagely 0.27%, 0.36%；In lean pork taken under the spinal column of a hog, epa, dpa content is averagely 0.13%, 0.25%；In lower limb buttockss meat, epa, dpa contain
Amount is averagely 0.44%, 0.596%.
Wherein, in matched group 2 beef, in shoulder elbow meat, epa, dpa content is averagely 0.20%, 0%；Epa, dpa content in chest and abdomen meat
Averagely 0.22%, 0.41%, in lean pork taken under the spinal column of a hog, epa, dpa content is averagely 0%, 0%；In lower limb buttockss meat, epa, dpa content averagely exists
0.35%、0.40%.
Claims (7)
1. a kind of for producing the high feature beef feedstuff of epa, dpa content it is characterised in that
Described beef feedstuff intends, by cattle normal diet and fermentation are micro-, the work(high for producing epa, dpa content that ball algae forms
Can property beef feedstuff；
Described fermentation micro- plan ball algae is obtained to inoculation Deshi Lactobacilluss fermentation in micro- plan ball algae solution.
2. a kind of feature beef feedstuff high for producing epa, dpa content according to claim 1, its feature exists
In,
Described fermentation is micro- to intend ball algae in concrete making,
(1) take micro- plan ball algae solution to prepare micro- plan ball algae solution fermentation medium, intend adding nan03 0.5g in ball algae solution to every l is micro-,
Mgso4 7h2o 4g, fes04 6g, sucrose 15g, natural ph value, sterilize at 150 DEG C 10min, is cooled to room temperature；
(2) add Deshi Lactobacilluss bacterium solution in the solution of step (1), mix homogeneously, then in 30 DEG C, under the conditions of 220rpm
Culture 24h.
3. a kind of feature beef feedstuff high for producing epa, dpa content according to claim 1 and 2, its feature
It is,
In micro- plan ball algae solution, algae cell density is 5x107cells/ml.
4. a kind of feature beef feedstuff high for producing epa, dpa content according to claim 1 and 2, its feature
It is,
Deshi Lactobacilluss bacterium solution inoculum concentration is 2%v/v.
5. a kind of feature beef feedstuff high for producing epa, dpa content according to claim 4, its feature exists
In,
Deshi Lactobacilluss bacterium solution Zhong Deshi living preparation of lactobacillus number is 1 × 108More than cfu/ml.
6. a kind of feature beef feedstuff high for producing epa, dpa content according to claim 1, its feature exists
In,
Cattle normal diet and fermentation micro- plan ball algae are higher for producing epa, dpa content than composition by the weight of 100:2-2.5
Feature beef feedstuff.
7. a kind of feature beef feedstuff high for producing epa, dpa content according to claim 1 or 5, its feature
It is,
The feature beef high for producing epa, dpa content is obtained after cattle normal diet and the direct mixing of fermentation micro- plan ball algae
Feedstuff.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610745356.7A CN106359930A (en) | 2016-08-29 | 2016-08-29 | Feed for producing high EPA and DPA content functional beef |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610745356.7A CN106359930A (en) | 2016-08-29 | 2016-08-29 | Feed for producing high EPA and DPA content functional beef |
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| CN106359930A true CN106359930A (en) | 2017-02-01 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201610745356.7A Pending CN106359930A (en) | 2016-08-29 | 2016-08-29 | Feed for producing high EPA and DPA content functional beef |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104470369A (en) * | 2012-07-17 | 2015-03-25 | 康奈尔大学 | Algal-based animal feed composition, animal feed supplement, and uses thereof |
| CN105112463A (en) * | 2000-01-28 | 2015-12-02 | Dsmip资产公司 | Enhanced production of lipids containing polyenoic fatty acids by very high density cultures of eukaryotic microbes in fermentors |
-
2016
- 2016-08-29 CN CN201610745356.7A patent/CN106359930A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105112463A (en) * | 2000-01-28 | 2015-12-02 | Dsmip资产公司 | Enhanced production of lipids containing polyenoic fatty acids by very high density cultures of eukaryotic microbes in fermentors |
| CN104470369A (en) * | 2012-07-17 | 2015-03-25 | 康奈尔大学 | Algal-based animal feed composition, animal feed supplement, and uses thereof |
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Application publication date: 20170201 |