CN106358744A - Selenium-rich tomato cultivation method - Google Patents

Abstract

The invention discloses a selenium-rich tomato cultivation method and belongs to the technical field of selenium-rich vegetable production. The selenium-rich tomato cultivation method comprises the steps that Human tomato No.2 undergoes secondary soaking and filtration, then are mixed with river sand after being washed and are covered with wet gauze for germination acceleration, sprouted tomato seeds are collected for standby application, brown sugar, sodium selenite and the like are taken for mixing, saccharomycetes is inoculated after sterilization, fermented culture is performed to obtain a fermentation solution, the solution is sprayed in smashed oat, moisture soil and the like are added after mixing to obtain a mixture, the mixture is added to a polyethylene bag, sealing is performed to obtain a culture medium, the culture medium, is flatly laid in a seedbed, standby sprouted tomato seeds are inoculated, a selenium solution prepared by selenium protein, seleninic acid and the like is sprayed from tomato growth to a seedling stage, a flowering stage and a fruiting stage respectively, watering is performed to prevent insects, and selenium-rich tomatoes are picked after maturing. The selenium-rich tomato cultivation method has the advantages that the cultivation cost is low, the yield of the obtained selenium-rich tomatoes is 15-23% higher than that of other cultivation methods, the selenium-rich content is as high as 0.08-0.10 mg/kg, and the selenium-rich tomatoes have good quality.

Description

A kind of breeding method of selenium-enriched tomatoes

Technical field

The present invention relates to a kind of breeding method of selenium-enriched tomatoes, belong to rich selenium vegetables preparing technical field.

Background technology

Selenium is trace element needed by human.Selenium participates in multiple selenoenzymes and Selenonic protein in synthesized human.Wherein paddy Guang Sweet peptide peroxidase, is catalyzed hydroperoxides in vivo or lipid peroxide is changed into water or various alcohols, eliminates Free radical, to biomembranous attack, protects biomembrane from oxidative damage；Selenium participates in constituting the de- iodine enzyme of iodate thyroid amino acid.Selenium Human immunity can be improved, promote the propagation of lymphocyte and the synthesis of antibody and immunoglobulin.Selenium to colon cancer, skin carcinoma, The kinds cancers such as hepatocarcinoma, breast carcinoma have the obvious effect suppressing and protecting, its mesostate methyl in body Enol has stronger active anticancer.

Vegetable is one of requisite food in people's diet, it is possible to provide multivitamin necessary to human body, Mineral and dietary fiber etc..The vitamin c of needed by human 90% and 60% vitamin a are all from vegetable.Therefore, carry out richness The applied research of selenium vegetable simultaneously carries out wideling popularize having important practical significance.The Se content of rich selenium vegetables is significantly higher than routine Vegetable, and found by research, the selenium in rich selenium vegetables is mainly existed with the form of organic selenium, and physiologically active is high, Yi Beiren Body absorbs, and can prevent and treat some human diseasess related to selenium, and biological selenium of taking food is the most safely and effectively selenium supplement side Formula.

Rich selenium vegetables include selenium-enriched tomatoes etc., and selenium-enriched tomatoes are not only nutritious, selling price is high compared with Lycopersicon esculentum, and Substantially, favored by consumers, supply falls short of demand in market for medical care effect.Apply the quality that selenium can improve Fructus Lycopersici esculenti, improve Fructus Lycopersici esculenti fruit The content of vitamin c, free amino acid and reducing sugar in reality, reduces nitrate content.Also can be by removing excessive free radicals, ginseng With energy metabolism, promote the raising of Tomato Root System growth promoter and improving activity of root system, this is conducive to enhancing body metabolism, raising Body vitality injures with resisting adverse circumstance.But selenium-enriched tomatoes also exist and yield poorly at present, poor quality, and selenium-rich content is low, high cost The problems such as.

Content of the invention

The technical problem to be solved:, poor quality low for current selenium-enriched tomatoes Se content, yield poorly, cost High the drawbacks of, there is provided a kind of Hunan Tomato No. 2 seed is filtered through secondary immersion, more scrubbed after mix with river sand, use wet yarn Cloth covers accelerating germination, and the tomato seeds collected after germinateing are standby, takes brown sugar, sodium selenate etc. to mix, and sterilizing is followed by Yeasts etc. and sends out Ferment is cultivated, and obtains fermentation liquid, is sprayed in the Herba bromi japonici of pulverizing, adds moisture soil etc. to mix to obtain mixture, load Polythene Bag after mixing Middle sealing, obtains culture matrix, is laid in seedbed, the tomato seeds after the standby germination of inoculation, in tomato growth to seedling stage, opens The selenium solution that florescence, each sprinkling of fruiting period are obtained by selenium protein, Monohydrated selenium dioxide etc., and note insect protected of watering, pluck rich after maturation Selenium Fructus Lycopersici esculenti.Selenium-enriched tomatoes of the present invention cultivate low cost, and selenium-rich content is suitable for human nutrition collocation, health delicious, plants survival rate Height, can be cultivated with establishing in large scale.

For solving above-mentioned technical problem, the present invention using technical scheme as described below is:

(1) choose 100～200 Hunan Tomato No. 2 seeds, be added in the warm water of 1～2l40～45 DEG C and soak, stir in immersion Filter after 6～8h, obtain the seed after immersion, then add it to soak in 500～700ml mass fraction 8% trisodium phosphate solution Filter after 30～40min, the seed deionized water being filtrated to get is cleaned 3～5 times, the river sand of the seed after cleaning and wash clean 1:2 in mass ratio mixes, and loads in earthen basin after stirring, and wet gauze is covered on mixture surface, puts into 26～30 DEG C At a temperature of accelerating germination, after seed germination, collect germinate after tomato seeds, standby；

(2) 30～50g brown sugar, 0.01～0.02g sodium selenate, 20～30g peptone, 10～15g Semen Maydis powder and 500 are weighed respectively ～800ml deionized water, after stirring mixing, sterilize at a temperature of 110～120 DEG C 5～10min, obtains culture fluid, in culture fluid Inoculation yeast bacterium, nitrogen-fixing bacteria and trichoderma, inoculum concentration is 5～10%, after inoculation at a temperature of 30～35 DEG C fermentation culture 4～ 6h, obtains fermentation liquid；

(3) weigh 50～70g Herba bromi japonici, pulverize after drying, in Herba bromi japonici powder, spray above-mentioned fermentation liquid, fountain height is Herba bromi japonici quality 10～12%, stir mixing 20～30min after sprinkling, sequentially add 200～300g moisture soil, 30～50g pig manure, 10～15g first Chitosan Oligosacchaides, 20～30g perlite, 20～30g bentonite, 10～15g wheat stalk granule, 3～5g cellobiose, 6～ 8gem mycopowder, obtains mixture after stirring mixing, fills this blend in Polythene Bag, seals, seal 15 after extracting air in bag Open after～20 days, obtain culture matrix；

(4) above-mentioned culture matrix is tiled to seedbed, thickness is 20～30cm, by the tomato seeds after standby for step (1) germination It is seeded in seedbed, treat that Seedling length, to 8～10cm, is carried out arranging Seedling by line-spacing 10cm × 10cm；

(5) weigh 30～40g selenium protein, 0.5～1.0g Monohydrated selenium dioxide respectively, add 500～600ml deionized water, stirring mixing Afterwards, add 30～50ml finless eel blood, stir mixing 20～30min, obtain selenium solution；

(6) respectively spray above-mentioned selenium solution once in tomato growth to seedling stage, florescence, fruiting period, seedling stage fountain height is 5～7g/ Strain, florescence fountain height is 8～10g/ strain, fruiting period fountain height is 3～5g/ strain, and the process in whole growth notes watering And insect pest preventing and controlling, the long harvesting to maturation, you can obtain selenium-enriched tomatoes.

Compared with additive method, Advantageous Effects are the present invention:

(1) breeding method of the present invention is simple, cultivates low cost, and gained selenium-enriched tomatoes yield is higher than other breeding methods 15～23%；

(2) selenium-enriched tomatoes selenium-rich content of the present invention is high, reaches 0.08～0.10mg/kg, suitable human nutrition collocation, has preferably Quality.

Specific embodiment

Choose 100～200 Hunan Tomato No. 2 seeds first, be added in the warm water of 1～2l40～45 DEG C and soak, side is soaked Side is filtered after stirring 6～8h, obtains the seed after immersion, then adds it to 500～700ml mass fraction 8% trisodium phosphate solution Filter after middle immersion 30～40min, the seed deionized water cleaning being filtrated to get 3～5 times, the seed after cleaning and wash clean River sand 1:2 in mass ratio mixing, after stirring load earthen basin in, and by wet gauze cover on mixture surface, put into 26 Accelerating germination at a temperature of～30 DEG C, after seed germination, collects the tomato seeds after germinateing, standby；Weigh 30～50g brown sugar respectively, 0.01～0.02g sodium selenate, 20～30g peptone, 10～15g Semen Maydis powder and 500～800ml deionized water, after stirring mixing, Sterilize at a temperature of 110～120 DEG C 5～10min, obtains culture fluid, inoculation yeast bacterium, nitrogen-fixing bacteria and trichoderma in culture fluid, Inoculum concentration is 5～10%, after inoculation at a temperature of 30～35 DEG C fermentation culture 4～6h, obtain fermentation liquid；Weigh 50～70g swallow again Wheat, pulverizes after drying, sprays above-mentioned fermentation liquid in Herba bromi japonici powder, and fountain height is Herba bromi japonici quality 10～12%, stirs mixed after sprinkling Close 20～30min, sequentially add 200～300g moisture soil, 30～50g pig manure, 10～15g chitin oligosaccharide, 20～30g Margarita Rock, 20～30g bentonite, 10～15g wheat stalk granule, 3～5g cellobiose, 6～8gem mycopowder, must mix after stirring mixing Compound, fills this blend in Polythene Bag, seals, sealing was opened after 15～20 days, obtained culture matrix after extracting air in bag； Above-mentioned culture matrix is tiled to seedbed, thickness is 20～30cm, the tomato seeds after standby germination are seeded in seedbed, treat Seedling length, to 8～10cm, is carried out arranging Seedling by line-spacing 10cm × 10cm；Weigh 30～40g selenium protein, 0.5～1.0g Monohydrated selenium dioxide respectively, Add 500～600ml deionized water, after stirring mixing, add 30～50ml finless eel blood, stir mixing 20～30min, obtain selenium Solution；Finally respectively spray above-mentioned selenium solution once in tomato growth to seedling stage, florescence, fruiting period, seedling stage fountain height be 5～ 7g/ strain, florescence fountain height is 8～10g/ strain, fruiting period fountain height is 3～5g/ strain, and the process in whole growth notes pouring Water and insect pest preventing and controlling, the long harvesting to maturation, you can obtain selenium-enriched tomatoes.

Example 1

Choose 100 Hunan Tomato No. 2 seeds first, be added in 1l40 DEG C of warm water and soak, filter after stirring 6h in immersion, obtain Seed after immersion, then add it in 500ml mass fraction 8% trisodium phosphate solution, soak filtration after 30min, filter The seed deionized water arriving is cleaned 3 times, and the seed after cleaning is mixed with the river sand 1:2 in mass ratio of wash clean, stirs Load in earthen basin afterwards, and wet gauze is covered on mixture surface, accelerating germination at a temperature of putting into 26 DEG C, after seed germination, collect Tomato seeds after germination, standby；Weigh 30g brown sugar, 0.01g sodium selenate, 20g peptone, 10g Semen Maydis powder and 500ml respectively Deionized water, after stirring mixing, sterilize at a temperature of 110 DEG C 5min, obtains culture fluid, inoculation yeast bacterium, fixed nitrogen in culture fluid Bacterium and trichoderma, inoculum concentration is 5%, after inoculation at a temperature of 30 DEG C fermentation culture 4h, obtain fermentation liquid；Weigh 50g Herba bromi japonici again, Pulverize after drying, in Herba bromi japonici powder, spray above-mentioned fermentation liquid, fountain height is Herba bromi japonici quality 10%, stirring mixing after sprinkling 20min, sequentially adds 200g moisture soil, 30g pig manure, 10g chitin oligosaccharide, 20g perlite, 20g bentonite, 10g wheat straw Stalk granule, 3g cellobiose, 6gem mycopowder, obtain mixture after stirring mixing, fill this blend in Polythene Bag, extract in bag Seal after air, sealing was opened after 15 days, obtained culture matrix；Above-mentioned culture matrix is tiled to seedbed, thickness is 20cm, will be standby It is seeded in seedbed with the tomato seeds after germinateing, treat that Seedling length, to 8cm, is carried out arranging Seedling by line-spacing 10cm × 10cm；Weigh respectively 30g selenium protein, 0.5g Monohydrated selenium dioxide, add 500ml deionized water, after stirring mixing, add 30ml finless eel blood, stirring mixing 20min, obtains selenium solution；Finally respectively spray above-mentioned selenium solution once in tomato growth to seedling stage, florescence, fruiting period, seedling stage sprays The amount of spilling is 5g/ strain, and florescence fountain height is 8g/ strain, fruiting period fountain height is 3g/ strain, and the process in whole growth notes pouring Water and insect pest preventing and controlling, the long harvesting to maturation, you can obtain selenium-enriched tomatoes.Breeding method of the present invention is simple, cultivates low cost, institute Obtaining selenium-enriched tomatoes yield is higher than other breeding methods 15%；Selenium-enriched tomatoes selenium-rich content of the present invention is high, reaches 0.08mg/kg, has relatively Good quality.

Example 2

Choose 150 Hunan Tomato No. 2 seeds first, be added in 2l43 DEG C of warm water and soak, filter after stirring 7h in immersion, obtain Seed after immersion, then add it in 600ml mass fraction 8% trisodium phosphate solution, soak filtration after 35min, filter The seed deionized water arriving is cleaned 4 times, and the seed after cleaning is mixed with the river sand 1:2 in mass ratio of wash clean, stirs Load in earthen basin afterwards, and wet gauze is covered on mixture surface, accelerating germination at a temperature of putting into 28 DEG C, after seed germination, collect Tomato seeds after germination, standby；Weigh 40g brown sugar, 0.02g sodium selenate, 25g peptone, 13g Semen Maydis powder and 650ml respectively Deionized water, after stirring mixing, sterilize at a temperature of 115 DEG C 8min, obtains culture fluid, inoculation yeast bacterium, fixed nitrogen in culture fluid Bacterium and trichoderma, inoculum concentration is 8%, after inoculation at a temperature of 33 DEG C fermentation culture 5h, obtain fermentation liquid；Weigh 60g Herba bromi japonici again, Pulverize after drying, in Herba bromi japonici powder, spray above-mentioned fermentation liquid, fountain height is Herba bromi japonici quality 11%, stirring mixing after sprinkling 25min, sequentially adds 250g moisture soil, 40g pig manure, 13g chitin oligosaccharide, 25g perlite, 25g bentonite, 13g wheat straw Stalk granule, 4g cellobiose, 7gem mycopowder, obtain mixture after stirring mixing, fill this blend in Polythene Bag, extract in bag Seal after air, sealing was opened after 18 days, obtained culture matrix；Above-mentioned culture matrix is tiled to seedbed, thickness is 25cm, will be standby It is seeded in seedbed with the tomato seeds after germinateing, treat that Seedling length, to 9cm, is carried out arranging Seedling by line-spacing 10cm × 10cm；Weigh respectively 35g selenium protein, 0.8g Monohydrated selenium dioxide, add 550ml deionized water, after stirring mixing, add 40ml finless eel blood, stirring mixing 25min, obtains selenium solution；Finally respectively spray above-mentioned selenium solution once in tomato growth to seedling stage, florescence, fruiting period, seedling stage sprays The amount of spilling is 6g/ strain, and florescence fountain height is 9g/ strain, fruiting period fountain height is 4g/ strain, and the process in whole growth notes pouring Water and insect pest preventing and controlling, the long harvesting to maturation, you can obtain selenium-enriched tomatoes.Breeding method of the present invention is simple, cultivates low cost, institute Obtaining selenium-enriched tomatoes yield is higher than other breeding methods 19%；Selenium-enriched tomatoes selenium-rich content of the present invention is high, reaches 0.09mg/kg, has relatively Good quality.

Example 3

Choose 200 Hunan Tomato No. 2 seeds first, be added in 2l45 DEG C of warm water and soak, filter after stirring 8h in immersion, obtain Seed after immersion, then add it in 700ml mass fraction 8% trisodium phosphate solution, soak filtration after 40min, filter The seed deionized water arriving is cleaned 5 times, and the seed after cleaning is mixed with the river sand 1:2 in mass ratio of wash clean, stirs Load in earthen basin afterwards, and wet gauze is covered on mixture surface, accelerating germination at a temperature of putting into 30 DEG C, after seed germination, collect Tomato seeds after germination, standby；Weigh 50g brown sugar, 0.02g sodium selenate, 30g peptone, 15g Semen Maydis powder and 800ml respectively Deionized water, after stirring mixing, sterilize at a temperature of 120 DEG C 10min, obtains culture fluid, inoculation yeast bacterium, fixed nitrogen in culture fluid Bacterium and trichoderma, inoculum concentration is 10%, after inoculation at a temperature of 35 DEG C fermentation culture 6h, obtain fermentation liquid；Weigh 70g Herba bromi japonici again, Pulverize after drying, in Herba bromi japonici powder, spray above-mentioned fermentation liquid, fountain height is Herba bromi japonici quality 12%, stirring mixing after sprinkling 30min, sequentially adds 300g moisture soil, 50g pig manure, 15g chitin oligosaccharide, 30g perlite, 30g bentonite, 15g wheat straw Stalk granule, 5g cellobiose, 8gem mycopowder, obtain mixture after stirring mixing, fill this blend in Polythene Bag, extract in bag Seal after air, sealing was opened after 20 days, obtained culture matrix；Above-mentioned culture matrix is tiled to seedbed, thickness is 30cm, will be standby It is seeded in seedbed with the tomato seeds after germinateing, treat that Seedling length, to 10cm, is carried out arranging Seedling by line-spacing 10cm × 10cm；Weigh respectively 40g selenium protein, 1.0g Monohydrated selenium dioxide, add 600ml deionized water, after stirring mixing, add 50ml finless eel blood, stirring mixing 30min, obtains selenium solution；Finally respectively spray above-mentioned selenium solution once in tomato growth to seedling stage, florescence, fruiting period, seedling stage sprays The amount of spilling is 7g/ strain, and florescence fountain height is 10g/ strain, fruiting period fountain height is 5g/ strain, and the process in whole growth notes pouring Water and insect pest preventing and controlling, the long harvesting to maturation, you can obtain selenium-enriched tomatoes.Breeding method of the present invention is simple, cultivates low cost, institute Obtaining selenium-enriched tomatoes yield is higher than other breeding methods 23%；Selenium-enriched tomatoes selenium-rich content of the present invention is high, reaches 0.10mg/kg, has relatively Good quality.

Claims (1)

1. a kind of breeding method of selenium-enriched tomatoes is it is characterised in that concrete breeding method is:

(1) choose 100～200 Hunan Tomato No. 2 seeds, be added in the warm water of 1～2l40～45 DEG C and soak, stir in immersion Filter after 6～8h, obtain the seed after immersion, then add it to soak in 500～700ml mass fraction 8% trisodium phosphate solution Filter after 30～40min, the seed deionized water being filtrated to get is cleaned 3～5 times, the river sand of the seed after cleaning and wash clean 1:2 in mass ratio mixes, and loads in earthen basin after stirring, and wet gauze is covered on mixture surface, puts into 26～30 DEG C At a temperature of accelerating germination, after seed germination, collect germinate after tomato seeds, standby；

(2) 30～50g brown sugar, 0.01～0.02g sodium selenate, 20～30g peptone, 10～15g Semen Maydis powder and 500 are weighed respectively ～800ml deionized water, after stirring mixing, sterilize at a temperature of 110～120 DEG C 5～10min, obtains culture fluid, in culture fluid Inoculation yeast bacterium, nitrogen-fixing bacteria and trichoderma, inoculum concentration is 5～10%, after inoculation at a temperature of 30～35 DEG C fermentation culture 4～ 6h, obtains fermentation liquid；

(3) weigh 50～70g Herba bromi japonici, pulverize after drying, in Herba bromi japonici powder, spray above-mentioned fermentation liquid, fountain height is Herba bromi japonici quality 10～12%, stir mixing 20～30min after sprinkling, sequentially add 200～300g moisture soil, 30～50g pig manure, 10～15g first Chitosan Oligosacchaides, 20～30g perlite, 20～30g bentonite, 10～15g wheat stalk granule, 3～5g cellobiose, 6～ 8gem mycopowder, obtains mixture after stirring mixing, fills this blend in Polythene Bag, seals, seal 15 after extracting air in bag Open after～20 days, obtain culture matrix；

(4) above-mentioned culture matrix is tiled to seedbed, thickness is 20～30cm, by the tomato seeds after standby for step (1) germination It is seeded in seedbed, treat that Seedling length, to 8～10cm, is carried out arranging Seedling by line-spacing 10cm × 10cm；

(5) weigh 30～40g selenium protein, 0.5～1.0g Monohydrated selenium dioxide respectively, add 500～600ml deionized water, stirring mixing Afterwards, add 30～50ml finless eel blood, stir mixing 20～30min, obtain selenium solution；

(6) respectively spray above-mentioned selenium solution once in tomato growth to seedling stage, florescence, fruiting period, seedling stage fountain height is 5～7g/ Strain, florescence fountain height is 8～10g/ strain, fruiting period fountain height is 3～5g/ strain, and the process in whole growth notes watering And insect pest preventing and controlling, the long harvesting to maturation, you can obtain selenium-enriched tomatoes.