CN106349283B - A kind of preparation method of high-purity phospholipid acyl serine - Google Patents
A kind of preparation method of high-purity phospholipid acyl serine Download PDFInfo
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- CN106349283B CN106349283B CN201610709931.8A CN201610709931A CN106349283B CN 106349283 B CN106349283 B CN 106349283B CN 201610709931 A CN201610709931 A CN 201610709931A CN 106349283 B CN106349283 B CN 106349283B
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- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 title claims abstract description 15
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 125000002252 acyl group Chemical group 0.000 title claims abstract description 12
- 150000003904 phospholipids Chemical class 0.000 title claims abstract description 12
- TZCPCKNHXULUIY-RGULYWFUSA-N 1,2-distearoyl-sn-glycero-3-phosphoserine Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(=O)OC[C@H](N)C(O)=O)OC(=O)CCCCCCCCCCCCCCCCC TZCPCKNHXULUIY-RGULYWFUSA-N 0.000 claims abstract description 37
- ZWZWYGMENQVNFU-UHFFFAOYSA-N Glycerophosphorylserin Natural products OC(=O)C(N)COP(O)(=O)OCC(O)CO ZWZWYGMENQVNFU-UHFFFAOYSA-N 0.000 claims abstract description 37
- 229920000344 molecularly imprinted polymer Polymers 0.000 claims abstract description 14
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 35
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 30
- 238000006243 chemical reaction Methods 0.000 claims description 20
- 229920000642 polymer Polymers 0.000 claims description 17
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
- ZCHPKWUIAASXPV-UHFFFAOYSA-N acetic acid;methanol Chemical compound OC.CC(O)=O ZCHPKWUIAASXPV-UHFFFAOYSA-N 0.000 claims description 10
- 239000000047 product Substances 0.000 claims description 9
- 239000003480 eluent Substances 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 238000000605 extraction Methods 0.000 claims description 6
- 239000012043 crude product Substances 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- STVZJERGLQHEKB-UHFFFAOYSA-N ethylene glycol dimethacrylate Substances CC(=C)C(=O)OCCOC(=O)C(C)=C STVZJERGLQHEKB-UHFFFAOYSA-N 0.000 claims description 5
- 238000004128 high performance liquid chromatography Methods 0.000 claims description 5
- MSDRNVBLURSBHC-UHFFFAOYSA-N n-ethylethanamine;methanol Chemical compound OC.CCNCC MSDRNVBLURSBHC-UHFFFAOYSA-N 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- OZAIFHULBGXAKX-UHFFFAOYSA-N 2-(2-cyanopropan-2-yldiazenyl)-2-methylpropanenitrile Chemical compound N#CC(C)(C)N=NC(C)(C)C#N OZAIFHULBGXAKX-UHFFFAOYSA-N 0.000 claims description 3
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims description 3
- 238000006116 polymerization reaction Methods 0.000 claims description 3
- DBCAQXHNJOFNGC-UHFFFAOYSA-N 4-bromo-1,1,1-trifluorobutane Chemical compound FC(F)(F)CCCBr DBCAQXHNJOFNGC-UHFFFAOYSA-N 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims description 2
- 238000010828 elution Methods 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 5
- 238000002474 experimental method Methods 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 210000004556 brain Anatomy 0.000 description 6
- 230000006870 function Effects 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 239000000178 monomer Substances 0.000 description 6
- 239000000020 Nitrocellulose Substances 0.000 description 5
- 230000009471 action Effects 0.000 description 5
- 239000004005 microsphere Substances 0.000 description 5
- 229920001220 nitrocellulos Polymers 0.000 description 5
- 239000012071 phase Substances 0.000 description 5
- 239000000499 gel Substances 0.000 description 4
- 206010013786 Dry skin Diseases 0.000 description 3
- 150000003926 acrylamides Chemical class 0.000 description 3
- 239000003431 cross linking reagent Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 125000000524 functional group Chemical group 0.000 description 2
- 210000005036 nerve Anatomy 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- YUFFSWGQGVEMMI-JLNKQSITSA-N (7Z,10Z,13Z,16Z,19Z)-docosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCCCC(O)=O YUFFSWGQGVEMMI-JLNKQSITSA-N 0.000 description 1
- YYPNJNDODFVZLE-UHFFFAOYSA-N 3-methylbut-2-enoic acid Chemical compound CC(C)=CC(O)=O YYPNJNDODFVZLE-UHFFFAOYSA-N 0.000 description 1
- 235000021294 Docosapentaenoic acid Nutrition 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000008499 blood brain barrier function Effects 0.000 description 1
- 230000036770 blood supply Effects 0.000 description 1
- 210000001218 blood-brain barrier Anatomy 0.000 description 1
- 210000004958 brain cell Anatomy 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000002975 chemoattractant Substances 0.000 description 1
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 description 1
- 229960001231 choline Drugs 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000010812 external standard method Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000006386 memory function Effects 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 150000008106 phosphatidylserines Chemical class 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 210000004509 vascular smooth muscle cell Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/08—Esters of oxyacids of phosphorus
- C07F9/09—Esters of phosphoric acids
- C07F9/10—Phosphatides, e.g. lecithin
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F222/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a carboxyl radical and containing at least one other carboxyl radical in the molecule; Salts, anhydrides, esters, amides, imides, or nitriles thereof
- C08F222/10—Esters
- C08F222/1006—Esters of polyhydric alcohols or polyhydric phenols
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J9/00—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof
- C08J9/26—Working-up of macromolecular substances to porous or cellular articles or materials; After-treatment thereof by elimination of a solid phase from a macromolecular composition or article, e.g. leaching out
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F222/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a carboxyl radical and containing at least one other carboxyl radical in the molecule; Salts, anhydrides, esters, amides, imides, or nitriles thereof
- C08F222/10—Esters
- C08F222/1006—Esters of polyhydric alcohols or polyhydric phenols
- C08F222/102—Esters of polyhydric alcohols or polyhydric phenols of dialcohols, e.g. ethylene glycol di(meth)acrylate or 1,4-butanediol dimethacrylate
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2201/00—Foams characterised by the foaming process
- C08J2201/04—Foams characterised by the foaming process characterised by the elimination of a liquid or solid component, e.g. precipitation, leaching out, evaporation
- C08J2201/042—Elimination of an organic solid phase
- C08J2201/0424—Elimination of an organic solid phase containing halogen, nitrogen, sulphur or phosphorus atoms
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2335/00—Characterised by the use of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by a carboxyl radical, and containing at least one other carboxyl radical in the molecule, or of salts, anhydrides, esters, amides, imides or nitriles thereof; Derivatives of such polymers
- C08J2335/02—Characterised by the use of homopolymers or copolymers of esters
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention provides a kind of preparation method of high-purity phospholipid acyl serine, the step of including preparing phosphatidylserine molecularly imprinted polymer and purifying phosphatidylserine, phosphatidylserine is purified using engram technology, obtain the phosphatidylserine that purity is more than 70%, preparation method is simple, experiment condition is gentle, and production cost is low, improves the economic value of product.
Description
Technical field
The present invention relates to a kind of preparation method of high-purity phospholipid acyl serine, more particularly to one kind to apply molecular engram skill
The method that art purifies phosphatidylserine.
Background technology
Engram technology is the process being transferred to various large biological molecules from gel in a kind of fixed matrix.Southern exists
The concept that first proposed molecular engram in 1975.He is become the DNA fragmentation of agarose gel electrophoresis separation in gel
Property becomes single-stranded, and then nitrocellulose (NC) film is placed on gel, puts water suction paper handkerchief above, utilizes capillary
Pipe action principle makes the DNA fragmentation in gel be transferred on NC films, makes solid phase chemoattractant molecule.It is loaded with DNA single chain molecules
NC films can is hybridized in hybridization solution with another kind with markd DNA or RNA molecule (i.e. probe), has complementary series
RNA or DNA be attached to and be present on the DNA molecular of NC films, shown through autoradiograph or other detection technique cans
The zone of hybrid molecule.Because this technology is similar to the ink marks absorbed with blotting paper on paper, therefore it is referred to as " blotting ",
It is translated into " engram technology ".
Multiple action point can be formed when template molecule (microsphere) contacts with polymer monomer, by polymerization process this
Kind effect will be memorized, and after template molecule removes, is formed in polymer and template molecule steric configuration phase
The hole with multiple action point matched somebody with somebody, such hole will have selection evident characteristics to template molecule and the like.
Phosphatidylserine, also known as composite nerve acid, English name Phosphatidylserine, abbreviation PS, by natural big
Beans oil expression residue extraction, it is the active material of cell membrane, is particularly present in brain cell.Its function mainly improves nerve
Cell function, the conduction of nerve impulse is adjusted, promote brain memory function, because it has very strong lipophilicity, energy after absorption
Enough run through blood-brain barrier and enter brain, play vascular smooth muscle cells of releiving, increase the effect of brain blood supply.Be described as after
Choline and one big emerging " intelligent nutrition element " after " docosapentaenoic acid " DHA.Expert thinks that this natural materials can help carefully
Cell wall keeps pliability, and can strengthen the efficiency of the neurotransmitter of transmission brain signal, helps brain high-efficiency operation, excites
The state of activation of brain.
Presently commercially available phosphatidylserine, pureness specifications are based on 20%, 40% and 50%, because its preparation process is complicated,
Experiment condition is harsh, and there has been no purity be 70% and the product batch of above specification is sold.
The content of the invention
The technical problems to be solved by the invention are to provide the high-purity phosphorus that a kind of operating process is simple, experiment condition is gentle
The preparation method of acyl serine, this method can produce high-purity phospholipid acyl serine in batches.Generally purity is more than or equal to
70% phosphatidylserine is referred to as high-purity phospholipid acyl serine.
The present invention technical solution be：
A kind of preparation method of high-purity phospholipid acyl serine is provided, comprised the following steps：
Step 1：The preparation of phosphatidylserine molecularly imprinted polymer；
1.1：A phosphatidylserine is added in reaction vessel, n-hexane is added to the reaction vessel, makes phosphatidyl
Serine dissolves；
1.2：The acrylamide of 1.5-2 parts is added into the solution of step 1.1,15- is sequentially added after stirring 0.5-1 hours
20 parts of ethylene glycol dimethacrylate and the azodiisobutyronitrile of 0.1-0.2 parts, are then filled with nitrogen into reaction vessel,
Heated sealed stirs 20-30 hours, obtains bulk polymer to 50-70 DEG C；
1.3：The bulk polymer that step 1.2 is obtained crosses 60-100 mesh sieves after crushing, and the powder of excessively complete sieve is loaded into color
Compose in post, be successively 15-20 with volume ratio:1 methanol-acetic acid solution, methanol, volume ratio 20-30:1 methanol-diethylamine is molten
Liquid and methanol rinse above-mentioned chromatographic column, collect the solid in chromatographic column, molecularly imprinted polymer is obtained after low temperature drying；
Step 2：Prepare high-purity phospholipid acyl serine；
2.1：The molecularly imprinted polymer obtained in step 1.3 is fitted into chromatographic column；
2.2：Low-purity phosphatidylserine is dissolved in n-hexane, obtains phosphatidylserine crude product solution；
2.3：The phosphatidylserine crude product solution that step 2.2 is obtained is added in the chromatographic column of step 2.1, with using volume
Than for 15-20:1 methanol-acetic acid solution elution, collects eluent；
2.4：The eluent obtained with n-hexane extraction step 2.3, collect and concentrate n-hexane phase, that is, obtain high-purity phosphorus
Acyl serine product.
Detection purity method be：Detected by HPLC with EISD, detailed process uses external standard
Method.
The beneficial effects of the invention are as follows：
Phosphatidylserine product of the purity more than 70%, preparation method letter can be obtained by the preparation method of the present invention
Single, experiment condition is gentle, and production cost is low, improves the economic value of product.
Embodiment
Embodiment one：
First, the phosphatidylserine for weighing 20g homemade 90% is added in reaction vessel, is added into the reaction vessel
100ml n-hexanes so that phosphatidylserine dissolves；Then, 30g acrylamides are added to the reaction vessel, stirs half an hour
After sequentially add 300g ethylene glycol dimethacrylates and 2g azodiisobutyronitriles, be filled with into reaction vessel close after nitrogen
Envelope, it is heated to 50 DEG C of constant temperature and stirs 20 hours, obtain bulk polymer.
60 mesh sieves are crossed after the bulk polymer is crushed, the powder sifted out is fitted into chromatographic column, is with volume ratio successively
15:1 methanol-acetic acid solution 200ml, methanol 300ml, volume ratio 20:1 methanol-diethylamine solution 200ml and methanol
300ml rinses chromatographic column, and template molecule, the function monomer of residual, crosslinking agent are rinsed well, and molecule print had both been obtained after 50 DEG C of dryings
Mark polymer.
The molecularly imprinted polymer that 100g is prepared is attached in chromatographic column；Then by 10g50% phosphatidylserine
It is dissolved in 100ml n-hexanes, the solution is adsorbed by the chromatographic column equipped with molecularly imprinted polymer, is with 300ml volume ratios
15:1 methanol-acetic acid elutes, and collects eluent, merges n-hexane phase, concentration, obtains height and contain with 150ml n-hexane extractions twice
Measure phosphatidylserine product.Detected through HPLC, content can reach 76.35%.
Embodiment two：
First, the phosphatidylserine for weighing 20g homemade 90% is added in reaction vessel, is added into the reaction vessel
150ml n-hexane dissolution phosphatidylserines；Then 35g acrylamides are added to the reaction vessel, after stirring 45 minutes successively
350g ethylene glycol dimethacrylates and 2.5g azodiisobutyronitriles are added, nitrogen is then filled with into reaction vessel, is sealed
Reaction vessel, is heated to 60 DEG C of insulated and stirreds 25 hours, obtains bulk polymer.
60 mesh sieves are crossed after the bulk polymer is crushed, the powder sifted out is fitted into chromatographic column, is with volume ratio successively
18:1 methanol-acetic acid solution 200ml, methanol 300ml, volume ratio 25:1 methanol-diethylamine solution 200ml and methanol
300ml rinses chromatographic column, and template molecule, the function monomer of residual, crosslinking agent are rinsed well, and molecule print had both been obtained after 45 DEG C of dryings
Mark polymer.
The molecularly imprinted polymer 100g prepared is attached in chromatographic column, then by 13g50% phosphatidylserine
It is dissolved in 100ml n-hexanes, the solution is adsorbed by the chromatographic column equipped with molecularly imprinted polymer, is with 300ml volume ratios
20:1 methanol-acetic acid elutes, and collects eluent, merges n-hexane phase, concentration, obtains height and contain with 150ml n-hexane extractions twice
Measure phosphatidylserine product.Detected through HPLC, content can reach 72.43%.
Embodiment three：
The phosphatidylserine for weighing 20g homemade 90% first is added in reaction vessel, adds 200ml to the reaction vessel
N-hexane dissolution phosphatidylserine, 40g acrylamides then are added to the reaction vessel, stir half an hour, then to the reaction
Container sequentially adds 400g ethylene glycol dimethacrylates and 3g azodiisobutyronitriles, then to after the reaction vessel inflated with nitrogen
Sealing, is heated to 70 DEG C of insulated and stirreds 30 hours, obtains bulk polymer.
60 mesh sieves are crossed after the bulk polymer is crushed, the powder sifted out is fitted into chromatographic column, is with volume ratio successively
20:1 methanol-acetic acid solution 200ml, methanol 300ml, volume ratio 30:1 methanol-diethylamine solution 200ml and methanol
300ml rinses chromatographic column, and template molecule, the function monomer of residual, crosslinking agent are rinsed well, and molecule print had both been obtained after 40 DEG C of dryings
Mark polymer.
The molecularly imprinted polymer 100g prepared is attached in chromatographic column, it is then that 6g50% phosphatidylserine is molten
In 100ml n-hexanes, the solution is adsorbed solution by chromatographic column, is 18 with 300ml volume ratios:1 methanol-acetic acid elutes, and collects
Eluent, merge n-hexane phase, concentration, obtain high content phosphatidylserine product with 150ml n-hexane extractions twice.Through
HPLC is detected, and content can reach 81.79%.
The present invention principle be：When microsphere (90% phosphatidylserine) and polymer monomer (acrylamide, second two
Alcohol dimethylacrylate, azodiisobutyronitrile) contact when can form multiple action point, will by this effect of polymerization process
Memorized, after microsphere removes, be formed matching with microsphere steric configuration in molecularly imprinted polymer
The hole with multiple action point, while contain precise alignment in hole with microsphere functional group it is complementary by function
The functional group that monomer provides, crude product phosphatidylserine, into hole, obtain height and contained by being contacted with molecularly imprinted polymer
Measure phosphatidylserine product.This molecularly imprinted polymer has selectivity just as lock to this key.This just assigns this
Special " memory " function of polymer, i.e., the similar natural identifying system of biology, such hole will be to template molecule and its class
There are selection evident characteristics like thing.
Claims (2)
- A kind of 1. preparation method of high-purity phospholipid acyl serine, it is characterised in that：Comprise the following steps：Step 1：The preparation of phosphatidylserine molecularly imprinted polymer；1.1：1 part of phosphatidylserine is added in reaction vessel, n-hexane is added to the reaction vessel, makes phosphatidylserine Dissolving；1.2：The acrylamide of 1.5-2 parts is added into the solution of step 1.1,15-20 parts are sequentially added after stirring 0.5-1 hours Ethylene glycol dimethacrylate and 0.1-0.2 parts azodiisobutyronitrile, nitrogen is then filled with into reaction vessel, seal 50-70 DEG C is heated to, 20-30 hours is stirred, obtains bulk polymer；1.3：The bulk polymer that step 1.2 is obtained crosses 60-100 mesh sieves after crushing, and the powder of excessively complete sieve is loaded into chromatographic column In, it is successively 15-20 with volume ratio:1 methanol-acetic acid solution, methanol, volume ratio 20-30:1 methanol-diethylamine solution and Methanol rinses above-mentioned chromatographic column, collects the solid in chromatographic column, and the polymerization of phosphatidylserine molecular engram is obtained after low temperature drying Thing；Step 2：Prepare high-purity phospholipid acyl serine；2.1：The molecularly imprinted polymer obtained in step 1.3 is fitted into chromatographic column；2.2：Low-purity phosphatidylserine is dissolved in n-hexane, obtains phosphatidylserine crude product solution；2.3：The phosphatidylserine crude product solution that step 2.2 is obtained is added in the chromatographic column of step 2.1, is with volume ratio 15-20:1 methanol-acetic acid solution elution, collects eluent；2.4：The eluent obtained with n-hexane extraction step 2.3, collect and concentrate n-hexane phase, that is, obtain high-purity phospholipid acyl Serine product.
- 2. the preparation method of high-purity phospholipid acyl serine according to claim 1, it is characterised in that：Also include step Three：The purity of high-purity phospholipid acyl serine is detected with EISD by HPLC.
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