CN106349023A - Process for extracting resveratrol from peanut sprouts - Google Patents
Process for extracting resveratrol from peanut sprouts Download PDFInfo
- Publication number
- CN106349023A CN106349023A CN201610733976.9A CN201610733976A CN106349023A CN 106349023 A CN106349023 A CN 106349023A CN 201610733976 A CN201610733976 A CN 201610733976A CN 106349023 A CN106349023 A CN 106349023A
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- China
- Prior art keywords
- resveratrol
- extracting
- extraction
- peanut sprout
- filtrate
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
- C07C37/68—Purification; separation; Use of additives, e.g. for stabilisation
- C07C37/685—Processes comprising at least two steps in series
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C37/00—Preparation of compounds having hydroxy or O-metal groups bound to a carbon atom of a six-membered aromatic ring
- C07C37/68—Purification; separation; Use of additives, e.g. for stabilisation
- C07C37/70—Purification; separation; Use of additives, e.g. for stabilisation by physical treatment
- C07C37/82—Purification; separation; Use of additives, e.g. for stabilisation by physical treatment by solid-liquid treatment; by chemisorption
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
The invention discloses a process for extracting resveratrol from peanut sprouts. The process includes: drying and crushing the peanut sprouts, ultrasonically extracting the resveratrol, and purifying to obtain resveratrol products. The process has the advantages that ethanol extraction is integrated with ultrasonic extraction, the ultrasonic extraction is used according to the feature that the resveratrol in the peanut sprouts is high in solubility in a weak-polarity solvent, the process is high in extraction efficiency, high in product purity and low in extraction cost and energy consumption, the solvent used in the process can be recycled, low in use amount and low in influence on environments, and the bioactivity of the resveratrol can be prevented from being destroyed during the extraction process.
Description
Technical field
The invention belongs to biological technical field and in particular to be a kind of from peanut sprout extract resveratrol technique.
Background technology
Resveratrol is a kind of plant that plant is mouldy or naturally secret goes out in plant body when being endangered by insect
Antitoxin, has control platelet aggregation, reduce serum cholesterol, effectively prevents antioxidation and heart disease, prevents low fat albumen
Matter oxidation and normal cell become cancerous cell, prevent the pathological effects such as malignant tumor hypertrophy, antiinflammatory.Function containing resveratrol
Property food is well received, and the market demand is very big.At present, extract from plant resourceses and isolate and purify resveratrol research more next
More paid close attention to by people.Abroad, mainly from Fructus Vitis viniferae and Semen Vitis viniferae, extract resveratrol, domestic with Polygonum cuspidatum Sieb. et Zucc be mainly
Raw material is extracted, but all suffers from extracting the problems such as plant material scarcity used, high cost.
Bud vegetable has many types, gradually familiar from the peanut-sprout of Semen arachidis hypogaeae germination at present.Semen arachidis hypogaeae germinates becomes colored
Sprout dish when, peanut sprout can produce resveratrol in case be subject to outside stimuluss, can be significantly increased with germination resveratrol.According to platform
The biology disciplinary study group research of gulf university shows, the oxidize-resistant physiology active substance especially Resveratrol content of peanut-sprout is up to
90 times of Semen arachidis hypogaeae, than red wine (every 100g contain 0.1～0.015) how tens times of even hundred times (6.2～9.1
).
Therefore extract the resveratrol in peanut-sprout, it is urgently that the extract containing high concentration resveratrol is used for food
The problem solving, but extracting method so far can lead to extract degeneration or thermal loss, and resveratrol is destroyed
Problem, reduces Resveratrol content in peanut-sprout extract and is unfavorable for using as food material.
Content of the invention
For solving above-mentioned technical problem, the present invention provides one kind can improve resveratrol extraction ratio, and processing step is simple, produces
Quality is good, can avoid extracting of resveratrol in the peanut sprout that extract degeneration or thermal loss, resveratrol are destroyed
Method.
A kind of technique extracting resveratrol from peanut sprout, its processing step is:
(1) material collection: manually destroy blade during selecting naturally to come off and plant in Fructus Vitis viniferae production process；By collect
Peanut sprout is cleaned, and keeps in Dark Place；
(2) pretreatment of raw material: add 2-10 weight portion Liquid nitrogen precooler to freeze 2-20 minute in peanut sprout, subsequently carry out mechanical activation comminution
Obtain leaf powder；
(3) ultrasonic extraction resveratrol coarse powder: the peanut sprout after pulverizing is put in alcohol solvent, using ultrasonic extraction,
Bulking value (g/ml) ratio of wherein peanut sprout and alcohol solvent is 1:2 3；55～65 DEG C of Extracting temperature, extraction time 40～
80min, extracts after terminating, extracting solution is filtered to get filtrate and filtering residue, and filtrate is concentrated and recycling design, using reclaim
Solvent extracts again to filtering residue, and Extracting temperature and time and first time extract identical, extract after terminating, extracting solution is filtered
Obtain filtrate and filtering residue, filtrate is concentrated and recycling design；After the concentrated solution extracting twice is mixed, after being dried, obtain white hellebore
Alcohol coarse powder；
(4) purification: resveratrol coarse powder is carried out static adsorption and eluting through hpd450a macroporous resin；Column chromatography condition is: on
Sample speed is 1.0ml/min, after fully adsorbing, first removes the big impurity of depolarization with 30% ethanol 3bv eluting, then with 95% second
Alcohol 5bv eluting, elution flow rate is 1.0ml/min；Resveratrol yield is more than 4mg/100g, and content is more than 25%.
Ultrasonic extraction described in above-mentioned steps (3) be 300 w power under supersound process 30 min.
Technical scheme adopts ethanol integrated with supersound extraction, according to resveratrol in peanut sprout in alcohol solvent
In the higher characteristic of dissolubility, the judder being produced using ultrasound wave, very high acceleration, strong cavitation effect, stir
The special effects such as mix, improves the impact velocity between material molecule, so that technical scheme is had the following characteristics that 1, extract
Efficiency high, extraction cost are low.Employ ultrasonic wave extraction, promote resveratrol to the dissolution velocity in Extraction solvent,
Thus greatly reducing the consumption of solvent, reducing extraction cost, improving extraction efficiency, being that industrialized production is laid a good foundation.With
Prior art is compared, and the extraction ratio of resveratrol can improve 2-4 times about, the purity of resveratrol product and prior art phase
Ratio improves 5-10 percentage point；2nd, the process conditions that the present invention extracts are gentle, it is to avoid resveratrol in extraction process
The destruction of biological activity, is suitable for the extraction with bioactive substance.3rd, energy consumption of the present invention is low.Because extraction time is short, extraction
The features such as temperature is low, solvent load is few, reduces energy consumption, decreases the discharge of waste.4th, due to used in extraction process
Solvent can recycle, solvent load is little, and the impact to environment is relatively low.
Specific embodiment
Embodiment
(1) material collection: the peanut sprout collected is cleaned, keeps in Dark Place；
(2) pretreatment of raw material: add 2-10 weight portion Liquid nitrogen precooler to freeze 2-20 minute in peanut sprout, subsequently carry out mechanical activation comminution
Obtain leaf powder；
(3) ultrasonic extraction resveratrol coarse powder: the peanut sprout after pulverizing is put in alcohol solvent, using ultrasonic extraction,
Bulking value (g/ml) ratio of wherein peanut sprout and alcohol solvent is 1:2 3；55～65 DEG C of Extracting temperature, extraction time 40～
80min, extracts after terminating, extracting solution is filtered to get filtrate and filtering residue, and filtrate is concentrated and recycling design, using reclaim
Solvent extracts again to filtering residue, and Extracting temperature and time and first time extract identical, extract after terminating, extracting solution is filtered
Obtain filtrate and filtering residue, filtrate is concentrated and recycling design；After the concentrated solution extracting twice is mixed, after being dried, obtain white hellebore
Alcohol coarse powder；
(4) purification: resveratrol coarse powder is carried out static adsorption and eluting through hpd450a macroporous resin；Column chromatography condition is: on
Sample speed is 1.0ml/min, after fully adsorbing, first removes the big impurity of depolarization with 30% ethanol 3bv eluting, then with 95% second
Alcohol 5bv eluting, elution flow rate is 1.0ml/min；Resveratrol yield is more than 4mg/100g, and content is more than 25%.
Ultrasonic extraction described in above-mentioned steps (3) be 300 w power under supersound process 30 min.
Claims (2)
1. a kind of technique of resveratrol of extracting from peanut sprout is it is characterised in that comprise the following steps that:
(1) material collection: the peanut sprout collected is cleaned, keeps in Dark Place；
(2) pretreatment of raw material: add 2-10 weight portion Liquid nitrogen precooler to freeze 2-20 minute in peanut sprout, subsequently carry out mechanical activation comminution
Obtain leaf powder；
(3) ultrasonic extraction resveratrol coarse powder: the peanut sprout after pulverizing is put in alcohol solvent, using ultrasonic extraction,
Bulking value (g/ml) ratio of wherein peanut sprout and alcohol solvent is 1:2 3；55～65 DEG C of Extracting temperature, extraction time 40～
80min, extracts after terminating, extracting solution is filtered to get filtrate and filtering residue, and filtrate is concentrated and recycling design, using reclaim
Solvent extracts again to filtering residue, and Extracting temperature and time and first time extract identical, extract after terminating, extracting solution is filtered
Obtain filtrate and filtering residue, filtrate is concentrated and recycling design；After the concentrated solution extracting twice is mixed, after being dried, obtain white hellebore
Alcohol coarse powder；
(4) purification: resveratrol coarse powder is carried out static adsorption and eluting through hpd450a macroporous resin；Column chromatography condition is: on
Sample speed is 1.0ml/min, after fully adsorbing, first removes the big impurity of depolarization with 30% ethanol 3bv eluting, then with 95% second
Alcohol 5bv eluting, elution flow rate is 1.0ml/min；Resveratrol yield is more than 4mg/100g, and content is more than 25%.
2. extract the technique of resveratrol from peanut sprout it is characterised in that above-mentioned steps (3) according to claim 1
Described in ultrasonic extraction be 300 w power under supersound process 30 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610733976.9A CN106349023A (en) | 2016-08-28 | 2016-08-28 | Process for extracting resveratrol from peanut sprouts |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610733976.9A CN106349023A (en) | 2016-08-28 | 2016-08-28 | Process for extracting resveratrol from peanut sprouts |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106349023A true CN106349023A (en) | 2017-01-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610733976.9A Pending CN106349023A (en) | 2016-08-28 | 2016-08-28 | Process for extracting resveratrol from peanut sprouts |
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| Country | Link |
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| CN (1) | CN106349023A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111296289A (en) * | 2020-03-12 | 2020-06-19 | 蚌埠学院 | Method for improving resveratrol content in peanut sprouts by cell suspension culture technology |
-
2016
- 2016-08-28 CN CN201610733976.9A patent/CN106349023A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111296289A (en) * | 2020-03-12 | 2020-06-19 | 蚌埠学院 | Method for improving resveratrol content in peanut sprouts by cell suspension culture technology |
| CN111296289B (en) * | 2020-03-12 | 2021-08-31 | 蚌埠学院 | Method for improving resveratrol content in peanut sprouts by cell suspension culture technology |
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170125 |
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| WD01 | Invention patent application deemed withdrawn after publication |